Biotronic ® Top3 - sfile.f

Biotronic
®
Top3
Triple action for improved performance!
Per4izer – the breakthrough in
animal growth performance
Naturally ahead
CHALLENGES
IN
ANIMAL
PRODUCTION
• high levels of productivity
• high-quality products
• bacterial challenge
Salmonella, E. coli, Campylobacter, Clostridia, Streptococcus…
Feed
Water
Environment
GI tract
ANTIBIOTICS
VS
NATURAL
PRODUCTS
 reduction of common bacterial burden
 improvement of digestibility
 kill beneficial microflora
 no effect on feed hygiene
 drug-resistant bacteria
Natural products as potential
alternative to antibiotics…
ACIDIFICATION
 Dietary supplementation by acidifiers:

reduces pH and buffer capacity 

reduces pathogens in feed and GIT 

stimulates growth promotion
Feed hygiene and GIT health is improved…
ENHANCEMENT
OF EFFICACY
 The efficacy of organic acids might be
improved…
Phytochemicals
Organic acids
combining them with phytochemicals!
PHYTOCHEMICAL. DEFINITION
A term “phytochemical” refers to compounds
produced by plants which have protective or
preventive properties.
Phytochemicals are found in fruits,
vegetables, beans, grains and other
plants.
There are more than 1,000 phytochemicals:
 Anti-oxidant: allyl sulfides, carotenoids, polyphenols
 Antibacterial: allicin, cinnamaldehyde
 Hormonal action: isoflavones (soy beans)
IMPROVING
Individual
products
EFFICACY OF PRODUCTS
Blends of
organic acids &
phytochemicals
Blend of organic acids,
phytochemical &
permeabilizers
PERMEABILIZERS. DEFINITION
Permeabilizers
 weaken the outer membrane (OM) of
Gram-negative bacteria
 facilitate other antimicrobials to enter
bacterial cells.
BACTERIA
STRUCTURE
Outer membrane of
Gram-negative bacteria
contains lipopolysaccharide
layer which makes bacteria
resistant to:
• hydrophobic antibiotics,
• detergents,
• bile salts,
• digestive enzymes.
BIOTRONIC® PRODUCT
LINE
Biotronic® Top3
Active ingredients:
Biomin® Per4izer
Phytochemical
Organic acids
Sequential
Release Medium
BLEND
OF ORGANIC ACIDS
Sequential
Release Medium
ORGANIC
ACIDS.
DEFINITION. MODE
OF ACTION
H+
H+
dissociated
H+ R-
undissociated
RH
H+
pH reduction
Antimicrobial effect
ANTIMICROBIAL
EFFECT OF ORGANIC
ACIDS
•pH reduction
uses
energy to restore pH
Intestinal
pH 5-6
pH 7
•Disruption of DNA and
protein synthesis
•Disruption of membranes
Bacteria is not able
to replicate
(Booth, 1985 – Stratford & Anslow, 1998 - Russel & Diez-Gonzales, 1998 - Roe et al., 1998 – Krebs et al.,
1983 – Cherrington et al., 1983 – Freese et al., 1973)
ANTIMICROBIAL
EFFECT OF INDIVIDUAL
ORGANIC ACIDS AGAINST
SALMONELLA
ANTIMICROBIAL
EFFECT OF INDIVIDUAL
ORGANIC ACIDS AGAINST
E.
COLI
PHYTOCHEMICAL
Sequential
Release Medium
CINNAMALDEHYDE
Cinnamaldehyde is the organic compound that
gives cinnamon its flavor and odor. It occurs
naturally in the bark of cinnamon trees and other
species of the genus Cinnamomum.
Cinnamaldehyde plays an essential role in the cell
division reducing the proliferation of potentially
harmful bacteria.
PROKARYOTIC
CELL DIVISION
Cinnamaldehyde
Inhibit FtsZ protein and GTPase
activity, and assembly into Z-ring.
Bacteria does not divide!
Domadia et al., 2007
Nature Reviews.
Molecular cell biology
FtsZ = filamentation temperature sensitive protein-Z
GTPase = guanosine 5-triphosphate
PER4IZER
Sequential
Release Medium
INNOVATION BIOMIN® PER4IZER
Biomin® Per4izer is the unique
permeabilizing substance identified to enhance
the antimicrobial effect of the organic acids and
the phytochemical against pathogenic bacteria.
1. Per4izer weakens
gram-negative bacteria
outer membrane
2. Per4izer facilitates entry
of organic acids and
phytochemical
3. Lethal injury of bacteria
IMPROVEMENT
OF ANTIMICROBIAL
ACTIVITY BY ADDING PER4IZER
IN
100
80
60
40
20
0
VITRO TRIAL WITH
BIOTRONIC® TOP3
CARRIER – SEQUENTIAL RELEASE
MEDIUM
Biomin® Per4izer
Phytochemical
Blend of organic
acids
Sequential
Release Medium
CARRIER
 Vermiculite:




phyllosilicate/sheet silicate
clean to handle
odorless
mold resistant.
Vermiculite exfoliated expands
in a free flowing powder which
is able to absorb different
substances and serve as carrier.
TRIAL
RESULTS IN VIVO
BW vs NC +7%, vs PC +11%
ADWG vs NC +8%, vs PC +16%
FCR vs NC -3%, vs PC -1%
BW vs NC +7%,
ADWG vs NC +8%
FCR vs NC -3%
EFFECT
OF
BIOTRONIC® TOP3
ON
GROWTH PERFORMANCE IN WEANED PIGS
Species : weaned piglets (days 28)
Duration: 56 d
NC = no growth promoter
Biotronic® Top3 = 1 kg/t
Negative control Biotronic® Top3
No. animals
Δ, %
n = 30
n = 30
Initial body weight, kg
8.75
8.72
/
Final body weight, kg
35.62a
37.69b
+6
Feed intake, g/animal
982
1028
+5
Daily weight gain, g
481a
517b
+8
FCR
2.04
1.99
-3
ROI = 11:1
Economical benefit 2.5 €/pig
a,b
Means with different superscripts differ significantly; P<0.05
COMMERCIAL
GERMANY
TRIAL,
CRAILSHEIM,
 Trial design
Treatments:
1) Negative control (NC) – no AGP or NGP
2) Treatment – Biotronic® Top3 1 kg/t
90 piglets weaned at 28 days of age/group
Duration of the trial: 56 days
Parameters:
Growth performance (BW, ADWG, FCR)
COMMERCIAL
GERMANY
TRIAL,
CRAILSHEIM,
 Results
Average daily gain
(g)
Body weight (kg),
84 days
4.6%
32.0
31.5
31.0
30.5
30.0
29.5
4.4%
460
454
450
31.5
440
430
30.2
434
420
Control group
Biotronic® Top3
group
Control group
Feed conversion ratio
2.6%
1.6
1.55
1.5
1.55
1.51
1.45
Control group
Biotronic® Top3
group
ROI = 9.8:1
Biotronic®
Top3 group
SCIENTIFIC
TRIAL,
HUNAN UNI, CHINA
 Trial design
Treatments:
1) Negative control (NC) – no AGP or NGP
2) Positive control (PC) – Colistin 100g/t + CTC 100g/t
3) Treatment (BTR Top3)– Biotronic® Top3 1 kg/t
Parameters:
Growth performance (BW, ADWG, FCR)
pH value in feed and GIT
Bacteria in GIT
Histology of jejunum
SCIENTIFIC
TRIAL,
HUNAN UNI, CHINA
 Results on growth performance
NC
PC
BTR
Top3
Δ, %
NC vs BTR
Top3
Δ, %
PC vs BTR
Top3
n = 32
n = 32
n = 32
Initial BW d35, kg
10.0
10.9
10.7
/
/
Final BW d91, kg
42.5a
43.2a
48.1b
+13
+11
FI, g/animal/day
1092a
1112a
1269b
+16
+14
DWG, g
580a
578ab
668b
+15
+16
FCR
1.88
1.92
1.90
+1
-1
1
2
/
No. animals
Mortality, no of
animals
a,b
Means with different superscripts differ significantly; P<0.05
SCIENTIFIC
TRIAL,
HUNAN UNI, CHINA
 Results on pH reduction in feed and GIT
NC
PC
pH in feed
5.78
5.73
5.59 
Buffer capacity in feed,
ml of HCl
21.7
20.1
13.2 
8.5
pH in stomach
4.28a
4.30a
3.67b 
0.6
pH in doudenum
5.39
5.35
5.75
pH in jejunum
6.53
6.58
6.42
pH in ileum
6.97
7.02
6.89
pH in colon
6.28
6.52
6.51
a,b
Means with different superscripts differ significantly; P<0.05
BTR Top3
0.19
SCIENTIFIC
TRIAL,
HUNAN UNI, CHINA
 Results on microbes and morphology of
GIT
NC
PC
BTR Top3
E. coli
6.302a
5.970b
5.873b
Coliforms
8.277a
7.977b
8.151b
Salmonella typhimurium
8.220a
7.941b
8.053ab
Lactobacillus
6.295a
6.172a
6.515b
Bifidobacteria
6.891a
6.878a
7.130b
Villi length, µm
418.33a
448.63ab
456.18b
Crypt depth,µm
192.37
188.77
187.15
Ileum, Log cfu/g
Histology of jejunum
a,b
Means with different superscripts differ significantly; P<0.05
CONTRIBUTION
TO FEED HYGIENE
 Agar diffusion test
The effect of Biotronic® Top3 on the growth inhibition of
mold and yeast was evaluated
RESULTS
Strains
Inhibition after 96h
Aspergillus fumigatus1
xxx
Fusarium proliferatum
x
Candida famata
xx
Candida glabrata
xx
Candida krusei
xx
Fusarium graminearum
xxx
Monascus ruber
xxx
Penicillium roqueforti
1Excellent
inhibition after 24h
x
PELLETING
TEST
Mixer:
 Bühler Paddelmixer: Mixing accuracy d =0.00001
 Mixing time dry (without fat added): 120 sec*
 Mixing time in total: 300 sec
Pelletiser:
 Bühler Serial number 10231288
 Type: DPAA 660.178
 Die plate: diameter of the openings: 3.5 mm, length 55
mm
Sampling:
 Samples taken were cooled on plastic plates before
packaging
 Samples were stored at 4°C until further analysis
PELLETING
TEST
Sampling:
3 samples of the pre-mixtures in order to determine
mixing accuracy
 3 samples from the experimental mixture after the
mixing process - without any additional treatment 
recovery of active ingredients
 After steam injection (and before the pelletising process)
3 samples were taken and cooled to room temperature
before packaging  indication of damaged caused by
the steam injection (hydro thermal stability)
 3 samples of feed pellets were taken and cooled to room
temperature before packaging  indication of damage
caused by steam injection and subsequent pelletising
process (hydro thermal-mechanical stability)
RECOVERY
(90°C)
OF
BIOTRONIC® TOP3
120
100
80
Flavouring agents
60
Organic acids
40
20
0
Control
Steam heated
Pelleted
How does
®
Biotronic Top3 work
with different antibiotics
CHLORTETRACYCLINE
120
Inhibition, %
100
80
60
40
20
0
E. coli O55:K59(B5)
E. coli O128:H2
Chlortetracycline
Salmonella enteridits
Salmonella typhimurium
Chlortetracycline + Biotronic® Top3
CARBADOX
120
Inhibition, %
100
80
60
40
20
0
E. coli O55:K59(B5)
Carbadox
E. coli O128:H2
Salmonella enteridits
Carbadox + Biotronic® Top3
Salmonella typhimurium
COLISTIN
120
Inhibition, %
100
80
60
40
20
0
E. coli O55:K59(B5)
Colistin
E. coli O128:H2
Salmonella enteridits
Salmonella typhimurium
Colistin + Biotronic® Top3
CONCLUSION
 Biotronic® Top3 has no adverse effects
on antibiotics
 Biotronic® Top3 has an additive inhibitory
effect effect with some antibiotics on some
bacterial strains
Usage of Biotronic® Top3 has no influence
on recommended dosage of antibiotics!
CONCLUSIONS
 Dietary supplementation
with Biotronic® Top3

Improved growth performance

Lowered pH and buffer capacity in feed

Had an acidification effect in stomach

Decreased pathogenic bacteria

Spared beneficial bacteria

Stimulated growth of villi

Contributed to feed hygiene
APPLICATION
Dose: 1 kg/t of feed for
swine
Swine: Pre-starter, starter,
fattening
Weaning diarrhea: for optimum
results Biotronic® Top3+ a
conventional acidifier or
probiotics.
.
Thank you for your attention!