Organized by - Comité des Plastiques en Agriculture
Transcription
Organized by - Comité des Plastiques en Agriculture
NEW AGRICULTURAL TECHNOLCffilES • 1 9 nd 2 9 4« INTERNATIONAL CONGRESS ON NEW AGRICULTURAL TECHNOLOGIES Puerto Vallarla, México (Nuevo Vallaría, Nayarit, México) April 20 to 23 ORGANIZED BY: m ( ) QftCcpn nam L. i li exportadora de plásticos agrícolas, s. a. de c. v. Government of the State of Nayarit é,AQU A FINI, oAp S. A. DE C. V. I IQUID CARBONIC FIBRAS PLASTICAS !>f.MÍ XICOS . A díC V. Organized by: Honorary President Rigoberto Ochoa Zaragoza Gobernador Constitucional del Estado de Nayarit, México President Technical Secretary Administrative Secretary Member Member Member Member Lic. J u a n J . García de Alba B. Dr. Gilberto Gómez Priego Miss. Josefina Monraz Lic. Mario Palacios Kaim Ing. Silvestre Pérez González Ing. Jacinto García Martínez Lic. Guillermo F e r n á n d e z Presentation / t is very gratifying to see how agricultural technology is spreading at an especially rapid rate which only three or four years ago would have been unheard of Last year's First International Congress of New Agricultural Technologies left a seed which has borne fruits in the fields of visionary growers who improved their crops; it also offered them the chance to achieve new goals and a more promising future. This is why the government of the State of Nayarit and the organizing companies decided to organize this Second Congress, with the firm intention of contributing in a real and feasible way to technologize the Mexican grower. We feel especially grateful to all our national and international speakers who, with excellent disposition, have shared their work and knowledge with all those aspiring to become better growers. We hope that the conferences as well as the exchange of ideas and expertise among the participants of this Second International Congress of Agricultural Technologies, serve to achieve a better agriculture and a more productive Mexico. Welcome! Lie. Juan J. Garcia de Alba President of the Organizing B. Committee O r g a n i z e d by: «ttcon )MW1 ) - i II Tri-Cal, Inc. Exportadora de Plásticos Agrícolas, S. A. de C. V. M R . MARK MCCASLIN 1029 Railroad St. Corona, CA. 91720, U.S.A. b (909) 737-6960 Fax (909) 734-1154 L i c . J U A N J . GARCÍA DE A L B A B . Av. Circuito Madrigal N s 1736 Zapopan, Jalisco, México « (3) 642 7542 Fax (3) 642 7342 ié AQUAFIM, S. A. DE c . v . e ^ Aquafim, S. A. de C. V. Liquid Carbonic de México, S. A. de C. V. I N G . SILVESTRE P É R E Z GONZÁLEZ Veracruz Ng 317 Poniente Hermosillo, Sonora, México « (62) 15 15 57 Fax (62) 16 28 52 L i c . GUILLERMO FERNÁNDEZ Biol. Maximino Martínez Ng 3804 Colonia San Salvador Xochimanca México, D. F. 02870 » (5) 341 2197 Fax (5) 396 8138 FIBRAS PLASTICAS Fibras Plásticas, S. A. de C. V. L i c . M A R I O PALACIOS KAIM Calle 1 A N2 92 y 29 Colonia San José de la Escalera « (5) 391 9175 Fax (5) 392 0819 Conferences Index World plasticulture Speaker: SR. PHILIPPE PRINTZ President of Comité Internacional des Plastiques en Agriculture Paris, France Design alternatives and selection of localized irrigation systems Speaker: I N G . D A N I E L VELÁZQUEZ DUARTE Aquafim, S. A. de C. V. Hermosillo, Sonora, México Sap analysis and fertigation management in horticulture with plastic mulch Speaker: D R . HÉCTOR BURGUEÑO Bursag, S. A. de C. V. Culiacán, Sinaloa, México Cultural vector control of virus: plant louse and white fly Speaker: M . C . OCTAVIO Pozo CAMPODÓNICO Instituto Nacional de Investigaciones Forestales y Agropecuarias Tampico, Tamaulipas, México Improvement and control of indoor horticulture Speaker: D R . J O S É L Ó P E Z GÁLVEZ Chief of "Las Palmerillas" Experimental Station Almería, España Corn plasticulture Speaker: in France ING. M . L E D U J E A N Centre National Du Machinisme Agricole du Genie Rural Des Eaux et des Fonets Francia Web vegetable as competitiones Speaker: cultivation tools ING. ALEJANDRO GÁLVEZ Invernamex Mexico Automatization and nutrition Speaker: of irrigation equipments ING. A V I IVANIR Chief of Training and Service in Latin America, Motorola of Israel Jerusalem, Israel Diseases and soil Speaker: solutions D R . ALBERT O . PAULUS Plant Pathology University of California Riverside, California, U. S. A. Intensive strawberry Speaker: production D R . EDGAR Q U E R O GUTIÉRREZ Oganización Bimbo-Frexport Zamora, Michoacán, México Solarization Speaker: in México D R . FLORENCIO J I M É N E Z DÍAZ INIFAP Torreón, Coahuila, México Drip irrigation and in citrics and vines Speaker: microspraying B E N A M I BRAVDO PH. D. Chief of the Division for Outside Studies of Horticulture and Fruit Growing, Faculty of Agriculture, Hebrew University of Jerusalem Jerusalem, Israel Intensification and conduction of tomato cultivation Speaker: D R . RODRIGUE N E L S O N VIGOUROUX Agro Industrias Campus Tequisquiapan, Querétaro, México A grower's attitude on of new technologies Speaker: aplication S R . SALVADOR GARCÍA GUTIÉRREZ San Quintín, Baja California Norte, México World plasticulture M R . PHILIPPE PRINTZ Comité Internacional des Plastiques en Agriculture MR. J . he idea of using plastic materials in agriculture first appeared some 50 years ago. Since then, much progress has been made in the utilization, conception and materials used, which in turn has brought about substantial plastic consumption in this field. This progress continues with new applications in many countries. New national committees have been created: Pakistan 1992, Israel 1993, PHILIPPE PRINTZ Algeria 1994... Applications vary from one country to another, depending upon whether climate conditions create a stronger need for saving water, for protection against the environment, or to strive for precocity and protection of crops after harvest. These techniques do not necessarily apply in every country. Some global statistics on world plasticulture Mulching Surface / areas! ha. Tonnage (t) Regions Min. Max. Min. Max. Western Europe 250,000 300,000 60,000 75,000 Eastern Europe 8,000 10,000 2,000 2,500 Africa/Middle East 8,000 10,000 2,000 2,500 America 180,000 200,000 45,000 50,000 Asia/Oceania 3'000,000 3'500,000 300,000 350,000 World total 3'446,000 4020,000 409,000 480,000 15 World plasticulture Regions Greenhouses S urface / areas / ha. Tonnage (t) PEbd PVC 115,000 1,500 30,000 Min. Max. Western Europe Eastern Europe 61,000 20,000 64,000 22,000 Africa/Middle East 15,000 8,000 17,000 10,000 25,000 15,000 182,000 286,000 190,000 200,000 303,000 385,000 America Asia/Oceania World total USA Canada 100,000 3,500 ha. 500 ha. Floating covers Europe 30 000 ha (against only 25,000 in 1987) USA 6,000 acres Microtunnels 54,000 in Western Europe 27,000 of which are in Italy and Spain Storage in silos 145-155 000 tons in Europe These numbers attest to the economical and social importance of plastic use. Let's examine more closely the application of plastic in some countries which will serve as examples. MR. PHILIPPE PRINTZ Plasticulture in México Key figures: ha 1. Mulching 2. Low tunnels 3. Walk-in tunnels 4. Wind-breaks 5. Greenhouses: seedlings 6. Greenhouses: ornamentals 7. Anti-hail nets 8. Micro-spraying 9. Direct covers 10. Drip irrigation orchards 11. Drip irrigation vegetables 12. Soil disinfection Total 3,982 3,790 113 100 91.4 532.72 4,794.24 11,135.00 17.28 18.300 10,108.5 520.0 53,484.14 % 7.44 7.09 0.21 0.91 0.17 1.00 8.96 20.82 0.032 34.2 18.69 0.97 100.0 t 1,392.97 1,664.90 361.6 28.0 298.88 1,658.3 3,472.81 9,464.75 7.08 14,460.05 980.96 178.6 33,968.81 % 4.10 4.90 1.06 0.08 0.88 4.88 10.22 27.66 0.021 42.57 2.89 0.53 100.0 Principal material used: polyethylene. Films: 60,000 t. Strings: 18,000 t. Main tendencies: Tubes and hoses. - Increase in mulched surfaces. In the above applications, polyethylene - Increase in floating covers (at present accounts for 95,000 t; PVC for 45,000 700 ha.) We noticed, as in many other t; polypropylene for 45,000 t; others, countries, the efficiency of these products 10,000 t. in fighting against crop enemies. Undoubtedly, plastic films are the most significant category, but it should be borne — Increase in drip irrigation. — Progress in the use of plastics for soil in mind that part of these films are used in silos, as covers (25,000 t) and as sterilization (520 ha.) stretchable wraps for round bulks of fodder (2,000 t). 10,000 t are used as films for Plasticulture in France microtunnels (lower t h a n 1 m high). Key figures: 4.5 million tons of plastics Between 14,000 and 18,0001 are used as consumed each year, 170,00 tons of which mulch, 5,000 t of which are photodegrapertain to specific agricultural applica- dable for corn growing. 7,5001 are used in production greenhouses and plantule tions divided between: 17 World plasticulture production. Perforated films are used as direct covers. Principal horticulture crops benefiting from plastic use: Greenhouse: tomato, cucumber, roses and flowers, lettuce. Microtunnel: strawberries, melon, carrot, red valerian. In the field, under mulch and floating covers: lettuce, melon, radish. Greenhouse films have a thermal effect giving plants maximum protection against spring frost. These long-life films serve for three or four crop cycles (films are placed at the end of summer to avoid UV degradation, especially during the months with highest insolation). Thickness ranges from 180 to 200 mm. Products are labeled according to regulations, so the consumer may identify them. Microtunnel films have a short life. To avoid heating during spring, they are frequently perforated with knives or heating devises to allow better ventilation. Thus, they cannot be used afterwards. These films are traditionally 80 mm thick. The introduction of LLDPE (Linear Low Density Polyethylene) brought about thinner films with the same mechanical properties. Thickness in these films may vary between 40 to 80 jam, though 60 is the usual. Thicker films afforded the best results as far as precocity (since they have the strongest thermal effect.) PVC films were once used for films, but their utilization is declining for several reasons: they're more expensive than PE based products, less resistant to the wind, and cause disposal problems. There microtunnel films are also regulated. Mulch films are very diverse in nature (color, thickness), but they have two main 18 objectives: black or opaque films are used to fight weeds, and clear films are used to heat the soil. Crops growing outside the soil, which are becoming increasingly important in greenhouses, require films to reflect the light in favor of better photosynthesis. Photodegradable films aren't used save for corn crops. However several hundreds of hectares of industrial tomatoes are covered with these films. Biodegradable films are still unavailable in the market. New techniques under development: - The use of stretchable films to wrap round fodder bulks. This technique is appropriate for animal breeding (goats and sheep) in small farms, and requires little investment. - Solar sterilization (solarization) of soil during summer seasons to avoid applying chemical agents which may leave traces in the vegetables grown later. - Floating non-webbed covers to avoid attacks from certain viral vectors. The idea is to find alternatives to treatments which aren't effective enough in spite of repeated (and costly) applications, and to guarantee the consumer the absence of chemical traces. Plasticulture in Spain Key figures: Total plastic consumption in the country: 2,200,0001. Agricultural plastics represent 7.3% (162,000 t). In order of importance: Films (greenhouses, mulch, tunnels, ponds), 36% Irrigation, 30% Materials used: LDPE, 51%; PVC 23% (irrigation); HDPE for boxes, screens; PP, 14,500 t. Main surfaces: MR. Mulch: + than 100,000 ha. Greenhouses: 28,500 ha. Principal crops: pepper, tomato, cucumber, melon and watermelon. 2,000 ha are solely dedicated to flowers, 300 of which are in the Canary Islands. Microtunnels: 17,000 ha (much of this surface is inside greenhouses to speed melon and especially watermelon crops). It is in Andalucía (Almería region, in southern Spain) where the largest surface cultivated under plastic films is located. Films most frequently used: Greenhouses: 90% PE long-lasting films. (The proportion in Andalucía is 70% "regular" and 20% thermal, and the reverse proportion prevails in the Murcia region.) Long lasting films are 180 to 200 pm thick. EVA films are used in cooler climates and with less sunshine than those in the southern part of the country. HDPE 94 g/m2 shades are particularly spreading in the Canary Islands (1,500 ha). 40-50 pm films are used for soil sterilization. Microtunnels (40-50cm wideandhigh): have covered a stable amount of land for some years now. Films are between 25 and 75 mm thick. Mulch: 55% of mulched surface is dedicated to cotton, where 12 pm films are applied. In the case of melon and tomato, PE films or mixes with LLDPE are used. Thickness ranges between 25 and 40 pm (i.e., 100-120 kg/ha). In asparagus crops (6-7,000 ha) 30-60 pm films measuring 1.5 m in length are used. This film has a side soil-filled pleat that avoids lifting due to wind. The other side is buried at the base of the bed so this film can be lifted and replaced whenever necessary. Pepper crops utilize 50-75 pm films, which are 3 to 4 meters wide. Irrigation: Represents 30% of agricultural plastic consumption (47,0001). There PHILIPPE PRINTZ are more t h a n 3,025 ha irrigated of irrigated crops, if we consider all existing irrigation systems. (650,000 ha are irrigated with sprinklers and 150,000 ha are drip-irrigated.) Pond waterproofing for water storage: During the past few years, this market has grown and now represents 5,000 t. (2,500 of PVC, 1,500 of HDPE, and 1,000 of LDPE. ) It is the answer for regions with scarce rainfall. Plasticulture in the United Kingdom Key figures: Greenhouses: 1,200 t of PE films for 1,000 ha of greenhouses. Microtunnels: 500 t. Thermal insolation in greenhouses: 6001. Floating covers: 2,700 t perforated films, 500 t un-webbed . Silo storage: 5,8001 of covers, 3,0001 of bags, 1,300 t of stretchable film. Wrapping: 8,0001 of PE and 2,0001 of PET (polyethylene terephtalate) Water deposits: 1,000 t. It should be noted that England, together with Belgium was among the first countries to adopt stretchable film to protect fodder balls after harvest. Climate conditions in these countries make it difficult to produce hay, while storage in silos allows production of good quality fodder which keeps up to one year. This technique is under development en many countries. Plasticulture in Germany Plastics used in agriculture: 150,0001, of which 45% is PE, 25% PVC, 13% PP, and 7% PS. Main application: film for silo storage, 50,0001. World plasticulture Greenhouse films: 400 t. Plastic greenhouses represent only +/- 15% of covered land- that is, 600 ha. Mulch: Mainly for asparagus, cucumber and strawberry (100 ha). Floating covers: 6,100 ha. Non-webbed covers are progressing noticeably. They are also used as protection against cold in vegetable crops, herbaceous plants and wood plants. Plant pots and trays: 360 million units for potted plants, 110 million for greenhouse plants, 400 million for plantules, 750 million for shrub plants. Plasticulture in India Another continent, other techniques, another culture; the need to feed close to one million human beings. Plasticulture in India doesn't have the same bases nor objectives as in Western Europe or North America. Promoting plasticulture is a priority and there are development centers all over the country, where experimentation is under way, especially in the realm of drip irrigation, sprinkling, mulching (tomato, potato, tea, strawberry, for yield increases which have reached 20 to 30% and water savings between 50 and 70%), water reserves, undercover crops (only the first stages of experimentation) and everything concerning post-harvest. Two million plastic boxes instead of wooden boxes have reduced wood consumption and contributed to protecting the remaining forests. Main existing applications: Hoses (100,000 tPVC) Outdoor harvest storage (5,0001LDPE) Greenhouse bags (20,000 t PE) Principal Actions under Way: Drip irrigation (3,0001) on an area that grew from 1,000 ha in 1983 to 26,000 ha 20 in 1992. Channel waterproofing (10,000 km already completed). Plasticulture in Morocco This North African country essentially applies plasticulture in greenhouse crops. Close to 4,500 ha are dedicated to garden vegetables, and 400 ha to floral crops, tomato, melon, peppers, string beans, strawberries and banana. Though Morocco has a favorable c l i m a t e , w h e n compared to o t h e r temperate-climate countries in Europe, crops here also tend to move to the south. Cover films, 220 (am thick, last for two years. Greenhouse structures sometimes have double walls to avoid temperature losses during the winter. Plasticulture in Japan Key figures: In 1989, out of44,800 ha of greenhouses, only 2,000 were made of glass. PVC is the most frequently used material to manufacture cover films (historical reason): 89% of greenhouses are covered with PVC, as well as 66% of the 54,000 ha of tunnels. Conversely, 85% of the 133,000 ha of mulched crops, use PE. Once more, garden vegetables occupy the greatest share of land: strawberries 15%, c u c u m b e r s 13%, melons 13%, tomatoes 11%. Regulations: a tendency; quality insurance In order to help growers make the right choice, several countries have established regulations in close collaboration with MR. manufacturers, distributors and users. These regulations help define adequacy for a given application. Products conforming to regulations can be labeled when there exists a qualification certificate (or quality label). Periodical control during the manufacturing process should be carried out by the manufacturer. Additional controls carried out by an independent laboratory will insure that labeled products truly conform to regulations. Though it is true that this research generates costs and that labeled products are a little more expensive than standard products, in many cases, growers can't afford the risk of using u n r e l i a b l e materials. At present, work is being done in Europe to establish a European regulation t h a t will replace existing domestic regulations. Plastics and the environment Sometimes plastics are criticized for environmental reasons. It's interesting to confirm, however, that these materials are important agents protecting the quality of the environment. Witness to this are mulch films which make it possible to avoid chemical herbicides altogether and save water by impeding soil evaporation, which also has a bearing on efficient fertilizer use, since there is no washing. Mulches are commonly utilized together with drip irrigation, the best system to optimize low water resources. Another example are films used for soil heating and sterilization which suppresses or at least decreases pathogen fungus, nematode and weed populations. Other witnesses are non-webbed covers which keep plant lice, and other pests PHILIPPE PRINTZ away from plants, and so avoid virus transmission or the effects of direct parasites which affect yield and quality. These covers have made cultivation possible, under plague conditions so severe that even agricultural spraying wouldn't help. Films used for solarization should also be mentioned, since they avoid chemical disinfectants. Agricultural plastic recycling represents several problems due to its inherent characteristics: wide diversity of materials and applications (and, evidently, geographical dispersal), as well as degree to which it becomes soiled. Nobody questions these factors, but research is under way to propose simple and cost-efficient solutions that will allow an environmentally sensible means of disposal. Until now, in many countries the garbage dump continues to provide the most widely used solution, since it is the cheapest and closest to the crops. This is certainly not the most ecological answer. (Can't something else be done with plastics instead of just burying them in some landfill?) Soon in many countries this will no longer be a feasible practice, since laws have been emitted in several nations (France, Germany), limiting the tons of waste that can go into a landfill. In the short term, the most satisfactory solution, intellectually speaking, seems to be recycling the material. Old films could be used to manufacture second generation products (garbage bags, films for silo storage). In practice, however, this solution must face present economical considerations, especially the low price of raw resin. Nevertheless, recycling is the solution for thick (100 pm) films. It is believed that in Europe today, 8,000 t of PE agricultural 21 World plasticulture films are being recycled. France accounts for an important share of this recycled plastic. Thinner films used for mulch and microtunnels (which become extremely soiled due to their thickness and contact with the ground) are better eliminated by incineration, whether in domestic waste incinerating plants which recover energy, or in plants with a high energy consumption, such as cement manufacturers. Burning in the open field evidently, is not part of the solution. Plasticulture: more perspectives for the future Thanks to national organizations 22 (Comepa in Mexico, CPA in France, GKI in Germany, etc.) and to all the research centers currently working in this field, the dialog between growers and manufacturers continues. Progress has been achieved both in the manufacture of the products, and knowledge of plant physiology. The products of the future will undoubtedly be more sophisticated: films selectively absorbing certain radiations, retaining infrared rays, films serving as a barrier for certain gases conditioning fruits and vegetables; degradable films, etcetera. Information on the latest in products a n d t e c h n i q u e s s h o u l d be quickly circulated. Design alternatives and selection of localized irrigation systems ING. DANIEL VELÂZQUEZ DUARTE A q u a f i m , S. A. d e C. V. INO. Introduction A V a t e r is an increasingly scarce resource, not only in quantity, but also quality. Since growers are the principal water consumers, they're obligated to use it in the most efficient way possible, within the economical considerations every production activity entails. Even in areas considered to have a healthy supply of irrigation water, illmanagement can bring about contamination problems which will have negative repercussions on the crops, mainly those located in lower areas. Localized irrigation is a system allowing application of filtered water and chemicals to the soil through a network of tubes and other specialized e q u i p m e n t called emitters. Water is taken to the source of each plant, eliminating carrying losses Irrigation method Localized irrigation (drip and micro-spray) Main spraying systems Movable irrigation systems Flood or row irrigation DANIEL VELÀZQUEZ DUARTE and minimizing evaporation and deep percolation, without submitting the plants to extreme conditions. The objective of localized irrigation systems, from t h e agronomic and engineering point of view, is to keep humidity between the soil saturation level, a fraction lower than field capacity, around the radicular area of the plant. This is why it's important to apply basic knowledge on water use so systems can be designed to meet t r u e crop requirements. Efficient application Different irrigation systems allow great losses in the amount of water used, since the volume of water applied by each system varies: Efficiency % 90-95 80 70 50 or less 25 Design alternatives and selection of localized irrigation systems Project capacity represents the maximum irrigation the system can supply, which is based upon the critical évapotranspiration forecast of the crop. Maximum water requirements will depend upon the following: 1. Climate conditions. 2. Crop development. 3. Rain distribution. 4. Soil water retention. 5. Type of crop. 6. Application efficiency. 7. Need for washing. System uniformity The uniformity of a localized irrigation system will depend upon: - Pressure differences arising in the hydraulic network due to losses on account of friction and the topography of the plot. - Uniform emitter manufacture. - Number of emitters supplying the plant. - Emitter response to changes in water temperature and pressure. - Variations in the performance of the emitter due to possible plugging and/or aging. - The effect of the wind when sprayers or micro-sprayers are in use. - Efficient manufacture of pressure regulators when they are the case. The degree to which each one of these factors can affect irrigation evenness has been established by Solomon (1985), from greatest to lowest importance as follows: - Obturations - Number of emitters supplying the plant. - Variation coefficient on the manufacture of the emitter. - Emitter discharge exponent. - Emitter sensitivity to temperature. - Pressure variations. - Variation coefficient on the manufacture of the pressure regulators. - Ratio between the loss of load in the main tube and the irrigating tube. - A m o u n t of d i f f e r e n t d i a m e t e r s making up the main line. Design The design of a localized irrigation system consists in detailed specification of every component and required practices to establish irrigation, operation and maintenance calendars. Design implies an integration of irrigation technology to an agricultural production system, taking into account, besides wholly technical aspects, agronomic, environmental, social and economical considerations. The object of designing irrigation is to achieve efficient water and nutrient distribution in the crop. The best measure of efficiency in an irrigation system is a uniform or even application. The following are the steps taken in the design of an irrigation system: - Selecting the type of system. - Estimating maximum water and nutrient demands. - Selecting the emitter (flow, type and spacing). - Selecting irrigation lines (diameter and thickness). - D i s t r i b u t i n g t u b e s and m a k i n g hydraulic calculations. - Selecting type of filter. - Selecting product injection equipment. - Selecting a retro-washing unit (manual or automatic). - S e l e c t i n g pumping equipment (centrifugal, vertical, submergible). PHYSC IAL ! CHARACTERS ITC I OF UME SOL I VOLUME Of WET SOIL FET iTR lRG tATKDN REQUERM I ENT5 BY PHENOLOGC IAL STAGE AN AT MAX M IUM DEMAND HYDRAUUC CALCULATO INS 1 CUMATE [ CALCULADON 27 Design alternatives and selection of localized irrigation systems Technical viability study For each one of the design alternatives, it's necessary to establish whether or not the system poses a practical irrigation solution by identifying its advantages and disadvantages such as: - Water application and humidity distribution in the soil. - Supply and quality of the water. - Cultural operations. - Supply and type of energy. - Labor requirements, including level of training. - Engineering services availability. - System reliability. - Natural resource and environment conservation. Cost analysis The next step is to estimate the following costs for every system: - Initial investment. - Fixed annual cost. - Annual operating expenses. - Total annual cost. This analysis should consider, among its annual operating expenses, the savings a localized irrigation system offers in agronomic and economical advantages. Perhaps the most important of these is that irrigation doesn't interfere at all with activities like cultivating, applying chemical or organic products, harvesting and watering at the same time, etcetera. Besides, the appearance of unwanted plants among the rows is inhibited, thereby reducing cultivation activities. This of course, is of immediate economical consequence, since crop maintenance r e q u i r e s a r e d u c e d i n v e s t m e n t in equipment and labor. 28 Estimate of required yield increase to cover irrigation costs To determine if the irrigation system is productive from the economical point of view, we must first calculate the increase in yield to pay for the irrigation system. This is difficult since it entails estimating the unit market price of the product under cultivation. Distribution of the tubes and hydraulic calculations Once the right kind of emitter has been selected and the load it will be working under is determined, the next step is to decide the distribution of the tubes in the system. Generally, this process begins with defining the placement of the main watering tubes and finally the supply tubes. This depends upon: - The distribution of the crop. - Topography. - Location of the water source. The supply should be calculated based upon certain economical criteria such as optimum diameter. Besides, these supply lines should answer to the following conditions: - Maximum water speed allowed in the tubes. - Moderate pressure in the tubes. - Pressure relief in the tubes. - Obstructions. Filtration systems An adequate filtration choice is based upon the content of organic and inorganic solids obtained through prior water analysis. Filters are always placed finer ones last, so the larger particles are the first to be removed. Every localized irrigation system must ING. DANIEL include a filter unit and a chemical water treatment unit, since any obstruction in t h e s y s t e m can r e s u l t in reduced application uniformity, which in turn decreases efficient water application. Obstruction problems can be classified, according the type, into physical, chemical and biological. The right filter system and accessories consist in an appropriate choice of their different elements based upon the features of the emitter, water quality and the operating conditions. The following are some of the filters available: 1. Sand filters. 2. Centrifugal separation filters. 3. Net filters. 4. Ring filters. Any option can be manually or automatically operated. Design alternatives There are different approaches to design, depending upon the criteria involved. For example, in a auto-balanced emitter its necessary to maintain a 20% pressure differential in order to obtain a 10% differential in expense; the idea is to achieve a 95% uniformity. Based upon this parameter, different distributions can be applied in supply and main tubes, as well as in cleaning lines which end in drain valves (thus eliminating the need for manual, line by line, draining ). These cleaning lines can be placed at the head or the inside of the plot, depending upon: - Cultivation work. - Size of the plot and length of the row. - Plot configuration. - Cost of the equipment. Once these aspects have been defined, we can talk about different designs for a localized irrigation project. VELAZQUEZ DUARTE I. Main line, secondary line with a pressure regulator and non-balancing emitter The basic construction of this system consists in two parallel lines, joined by a swing, harboring the pressure regulator. In the field, its adequate for flat or uniformly slanting plots, even steep ones, since this system allows the plot to be divided into small regulated sections, thus reducing the impact of the slant. II. Main line with a manual valve to regulate incoming pressure and a non-balancing emitter This system eliminates several connections, and the regulators, which makes it a price alternative for level plots. The decision to design tubes where the incoming pressure drop is controlled by manual regulating valves (meeting specific quality r e q u i r e m e n t s for chemical application, resistance to handling and continuous field operation), and loss of friction caused by the diametrization of the tubes, is only recommended for flat, uniform or very moderately slanted fields. The operation of this system requires the person in charge of irrigation to calibrate the valves with a gage by opening and closing them, whatever the case, to set designed work pressure at the entrance of each plot. III. Main line with a hydraulic pressure regulating valve and non-balancing emitter The principal objective of this alternative is to facilitate operation and insure a specific water volume applied to the crop, cutting time and failures associated with manual pressure calibration. Design alternatives and selection of localized irrigation This design basically consists in supply tubes, main tubes and regulating valves, whose function is to keep a steady incoming pressure in the plot according to hydraulic design (notwithstandingpossible pressure changes in the supply tubes), through a regulating mechanism placed on the outside of the valve. This mechanism requires only one calibration to set a definite and correct pressure in the plot. For irrigation changes, the valves only need be completely open or shut. Another advantage is that these valves can be used in the event complete automatization of the irrigation system is desired, since they allow the installation of remote control (solenoid) units for multicable, mono-cable or radio control equipments. IV. Main line with a manual or hydraulic valve and balancing emitter In plots with irregular or rough topographies, the balancing emitter can satisfy the need for efficient and uniform water application. Its hydraulic mechanism has caused an impact on the development of different production areas whose topography didn't permit the use of non-balancing emitters at a reasonable cost. The uniformity of this system doesn't depend upon regulating valves or tubes. All emitters are balancing and capable of providing the same nominal expense in the field, regardless of their location. The function of the valves is to merely section; that is to open or interrupt the flow into the field in operation. Designs with a hydraulic valve (non regulating) make future automatization of the system possible. Besides, with this kind of emitter, 30 systems designs for level slanting plots are feasible, since its possible to have longer watering lines where cultivation practices allow it and obtain up to 95% uniformity, with a simpler design which saves on equipment maintenance and operation. The balancing emitter owes its benefits to its even distribution, superior to any other type of emitter, thanks to its pressure compensation design. In some cases, this is achieved by reducing the output hole, and in others, through pressure differentials between the watering line and the emitter. Both balancing emitters offer distinct advantages. System selection Once the different and economically feasible alternatives have been studied, a final decision on the irrigation system will also depend upon the producer's farming criteria and practices. Once the adequate choice has been made (one which will meet all the requirements of the crop ), its a good idea to make an in-depth analysis of the investment comparing the initial cost of the irrigation equipment, against energy consumption and operating expenses, since these are directly interrelated. The lower the pressure in the equipment, the higher the initial investment and the lower the permanent energy consumption. Keeping this in mind, it's a good idea to design guarding relationships between the diameters of the lines and total dynamic load of the pump to achieve an economical balance for the producer. An equally important aspect which complements the choice of a system is the purchase of accessories such as: Acid and fertilizer injector, chemical mixing t a n k s , e v a p o r i m e t e r and/or atometer, tensiometers, humidity sensors, ING. DANIEL volumetric meter, automatic filter cleaning systems, etcetera. These afford optimum operation of the irrigation system and thereby, the greatest benefits from this technology. An i m p o r t a n t factor in the final selection are system operation and maintenance considerations. These shouldn't require specialized labor, where there's none readily available. Besides all the components making up the irrigation system should be accesible and as near as possible to the actual location of the VELAZQUEZ DUARTE equipment. Selection is a process to determine the irrigation system to be used in an agricultural operation. Though the main criteria is normally based on the cost ofthe different alternatives, other factors can lead to a choice which isn't the least expensive. In general terms, the system should satisfy maximum crop water and nutrient requirements, with a uniformity equal to or over 90%, where available labor can handle and keep the system. Design alternatives and selection of localized irrigation systems Bibliography AQUAFIM. Service Manual, M . Micro-irrigation Manual, 1990. BOSWELL, J . 32 R . and A B R E U , H . localizado, Spain, 1992. LÓPEZ, J . 1994. J. M. Riego Micro-irrigación, 1992. Design RANAHAN, Z . F E D R O . Sap analysis and fertigation management in horticulture with plastic mulch D R . HÉCTOR BURGUEÑO B u r s a g , S. A. d e C. V. DR. O n e of the problems facing developing countries is lack of food. This is due to factors such as demographic growth and poor distribution of natural resources, water, climate and suitable farming land. Mexico is not free of these conditions and needs to increase its production. Slightly over 50% of the Mexican territory has a semi-arid to arid climate, with scarce rains and saline soils. Its necessary to improve irrigation and fertilizing techniques, in order to increase the availability of cultivable land by increasing yields. This can be made possible by adapting new methodologies; it is therefore important for us to develop the practice and utilization of mulch crops, managed through conducted irrigation systems and fertirrigation. The combination of new agricultural technologies also allows more efficient use of raw materials, and at the same time improves harvest quality. Crop m a n a g e m e n t w i t h new a g r i c u l t u r a l technologies h a s been implemented with the help of: - Drip irrigation. - Plastic mulch. - Tensiometers. - Soil solution extractors. - Fertirrigation. - Sap analysis. HÉCTOR BURGUEÑO Mineral analysis of sap from conductive tissue I. Why analyze conductive tissue? Plant analysis con be considered a classical means to control fertilizing and nutrition of the vegetables grown. The most commonly used reference to carry out this control is the leaf. Evidently its generalization under the concept "foliar diagnosis" arises from the fact that it's an easily accesible part of the plant, and its position or level in the plant is easy to determine. However it's not evident that this is the best option. In effect, in the case of vegetables which produce an important biomass in a s h o r t period, foliar composition varies slowly with respect to the rate of growth of the plant. Leaves aren't, therefore, a sensible enough reference organ to evaluate the nutritional status of these plants. Quite the contrary, it's evident that conductive tissue (stems, petioles, axillar sprouts) permanent and directly related to the "supply source" (radicular system) and the "consumers" of the mineral elements (leaves and fruits) constitute a more apt indicator. Inspired by the work carried out by Sap analysis and fertigation management in horticulture with plastic Routchenko, more than ten years ago, Dr. Philippe Morard, director of the Plant Physiology Laboratory of the National Agricultural School of Toulouse (ENSAT), France, designed a quick and simple method to follow plant nutrition through sap analysis of the conductive tissue. - Quick, since from an analytical point a view, tests are made directly and without preparing extracts from the conductive tissue. - Simple, because this method requires axillary sprouts or petioles during the entire cultivation cycle. These analysis concentrate mostly on the main kinds of chemicals present in conductive tissue fluids: N03-; NH4+; H2P04-; K+; Mg++; Ca++ Besides Na, Fe; Zn; Cu; Mn; pH and electric conductivity of the sap. According to Dr. Morard, the global amount of an element present in the plant fluids reflects its adsorption conditions, while ionic fraction of a mineral element constitutes a surplus or reserve from which the plant supplies itself according to its needs. Sap analysis technique is b e t t e r adapted to the follow-up of a rapidly growing annual plant than foliar analysis. II. Why carry out a regular follow-up on plant nutrition? Since this technique is important because of its speed and sensitivity to variations in nutritional flow, it also entails 36 mulch new concepts on the interpretation of these analysis. In effect, it's a well known fact that the flow and concentration of mineral elements found in conductive tissue vary with regards to several parameters, particularly: - Insolation. - Time of day. - Age and developmental stage of the plant. - Temperature and relative humidity of the environment. Based upon the above, and as opposed to foliar diagnosis, this technique makes it impossible to refer to standard optimum values; in other words, to vulgarize "concentration parameters" for the mineral elements of plants. T h i s m e t h o d , which h a s been experimented and corroborated, consists in continuous control of macro and microelement concentrations during the development cycle, and especially during the production stage. For example, in tomatoes of a specific growth, 6 to 10 samples are taken between the blooming of the first cluster to the end of the harvest. These are quickly analyzed (48 hours), and the results are recorded on a graph. That allows us to follow the evolution of each element analyzed in function of the time, and so obtain a profile of the nutritional status of each irrigation plot. Fertirrigation will not be practiced based upon preestablished standards, but according to the tendency in concentration evolution of the two subsequent samplings. DR. HÉCTOR HIGH VALUES • BURGUEÑO • •WITHOUT INTERVENTION U1 LOW VALUES HIGH VALUES •ELEMENT SUPPLY LOW VALUES HIGH VALUES •DECREASE OF SUPPLY LOW VALUES In Europe, high and low value scales were experimentally established. The low value is the threshold of deficiency (determined by a crop outside the ground for every element); the high value is the threshold of concentrations leading to antagonism. NO ' 3 Grow stage Fructification stage K From the work done by doctors Morard and Roucolle, we have o p t i m u m concentration variation parameters for mineral elements regarding physiological status of greenhouse cultivated tomato. These are provided in the following table only as an indicator. (Climate and photoperiod conditions are different to those prevailing in our country.) + NH 4 PO 4 H 2 ++ ++ Ca Mg Max. 3,300 4,400 180 320 150 170 Mean Min. 2,000 800 3,900 3,400 110 50 200 130 80 20 110 60 Max. 2,900 4,400 100 200 90 170 Mean Min. 2,000 1,100 3,800 3,500 80 50 180 130 45 20 120 80 Sap analysis and fertigation management in horticulture On the other hand, during my doctorate work in France (1985-1987), while working with greenhouse tomato plants, we studied the influence of different cultivation systems on the mineral nutrition of the plants, both in uncovered and mulched soils, as well as in off-ground substrata (volcanic rock, peat, expanded clay and hydroponic). We found that in all the treatments used, as long as acceptable mineral nutrition is maintained, no significant differences in mineral content of the sap can be seen due to type of crop or substratum. Fruit quality doesn't depend upon the cultivation systems but on adequate fertirrigation. III. Importance of this method to nutritional control in vegetable crops For more than ten years, French horticultural producers control fertilization of their crops, mainly tomato and cucumber, aided by this method of permanent analysis of sap drawn from conductive tissue. This methodology is being commercially exploited in France by the Europe Sols, S.A. company, which pays royalties to the ENSAT Laboratory. What does this n e w technique provide growers? Better control over mineral nutrition of the plant. Intensively managed crops (plastic mulch, drip irrigation, fertirrigation, greenhouses, etcetera) display a very important capacity for biomass synthesis. In full production, these plants can manufacture the equivalent of their own fresh weight every two days. (The composition of the leaf varies slowly with respect to the rate of growth.) Underthese conditions, permanentcontrol of a balanced mineral nutrition is w<ith plastic mulch indispensable. An appropriate fertirrigation formula can, in effect, become unbalanced within a few days according to plant needs. Such imbalances can result in: - Reduced yield. - Inferior product quality. This is why its advisable to practice sap analysis every 10 to 15 days from initial blooming until full production. IV. Sap analysis in Mexico We've used this technology based upon the following: - Sap from plants of a given species, placed under identical environmental conditions, shows no a p p r e c i a b l e differences in chemical composition. -Changes affecting element availability in the soil generally translate into modifications in t h e chemical composition of the sap. These modifications however, can be partially masked by the intervention of other phenomena; especially variations in growth rate and by interaction produced at different levels. - Among the ecological conditions, intensity and duration of sunlight are factors which can substantially modify, activating or reducing plant metabolism, hydric nutrition conditions contribute to establish the chemical composition of the sap characteristic to the mineral nutrition of the plants. Sudden changes, especially irrigations after a drought, also affect the mineral nutrition of the plants; in most cases, temporarily. - High yields correspond to a certain chemical status in the sap. However, a given favorable composition will not necessarily lead to high yields due to possible depressing external factors. - Sap and foliar analysis provide a converging look at the plants' nutritional status, but ultimately, the wide variation in mineral elements present in the sap is DR. more important than the variation of these elements in the leaf. - When searching for the source of anomalies, sap analysis provides a way to discover them, when they're caused by malnutrition. - These methods are precise enough to allow the nutritional status of a crop to be classified as: insufficient, good or excessive. V. Analytical process and operating standards Sampling should be carried out three hours after dawn. We've done so from nine to eleven at the most. Sampled plants must be representative of the maj ority of the population, especially as far as development and color. The number of organs per sample will depend upon the plant species and the juiciness of its conductive tissue. It shouldn't be less that thirty. The parts of the plant sampled are axillar sprouts or petioles, depending upon the species. The position of the organ to be sampled will be the fifth rank of growth after the growth apex. When sampling we carry along: - an ice filled ice box, - 250 cc glass jars with fight fitting plastic lids, - Anhydrous ethyl ether, and - a permanent ink marker. The samples are placed inside the jar with a little bit of ether (30 to 50 cc) to stop tissue metabolism. Afterwards they are kept cold in the ice box up to two hours maximum after the sampling has ended. Samples are frozen in the lab to completely stop their metabolism. The sap is then extracted by pressure (squeezing), and directly analyzed. N0 3 " and H 2 P0 4 " analysis are done by HÉCTOR BURGVEÑO colorimetry. Other elements are analyzed with the atomic adsorption spectrophotometer. Results are expressed in parts per million (ppm). During t h e 1993-1994 season in Culiacán valley and the Ruiz Cortines area in Sinaloa, we carried out numerous foliar and sap analysis in crops such as tomato, bell pepper, squash, cucumber and melon. We were able to corroborate the better indicative response of the true nutritional status of the crops through the variations in mineral element concentrations in the sap, whereas foliar analysis showed no significant variations. Out of the results from more than 500 sap analysis practiced in different irrigation plots during different stages of development, we present the following variations in mineral element concentrations in the sap of tomato and bell pepper crops. These concentrations are given in three levels classified as: Level 1: Mineral concentration of plants showing optimum growth levels, with a tendency towards higher concentrations indicating excessive fertilizing. Level 2: Mineral element concentration of normally developing plants. We estimate that optimum nutrient concentrations in the sap, which culminate in a crop exploiting a good share of its genetic production capacity, are located between the levels described above. Level 3: Concentrations close to nutrient deficiency, making a timely intervention necessary. It should be noted that the numbers that follow do not represent the maximum or minimum values detected, nor their averages. These are mineral element concentrations deemed sufficient to carry out adequate crop nutrition management through fertirrigation practices. 39 Sap analysis and fertigation management in horticulture with plastic mulch Tomato sap analysis Mineral element concentrations (ppm) N, NO H PO 3 2 4 + ++ ++ + K Ca Mg Na Zn Cu Fe Mn pH C.E. Level 1 1,300 280 4,500 200 270 50 2 3 1.3 3 5.7 15 Level 2 1,000 200 3,600 80 200 30 1.2 1.6 1 2 5.6 14 Level 3 850 170 3,300 50 180 10 0.6 0.8 0.4 0.7 5.4 11 Bell pepper sap analysis Mineral element concentrations (ppm) N, NO H PO 3 2 4 + ++ ++ + K Ca Mg Na Zn Cu Fe Mn pH C.E. Level 1 1,700 280 7,660 80 700 70 3 5 2 6 5.6 21 Level 2 1,200 220 7,000 40 550 30 2.5 3 1.5 5 5.4 20.5 Level 3 900 160 6,500 30 400 18 1 1 0.5 2.5 5.3 19 We can briefly conclude that foliar analysis report data about what already h a p p e n e d to t h e crop, w h e r e a s a n u t r i t i o n a l follow-up based upon variations in m i n e r a l element concentrations, indicate what's happening at that moment. Fertirrigation crop management is based upon the application of fertilizers with respect to crop density; nutrients provided in grams per plant per day. Its very important to know the initial nutritional levels of the soil, as well as the concentrations and types of salts present in the irrigation water, since this will enable us to make a good selection of the chemical content of the fertilizers to be 40 used, and reduce the risk of having plugged up drip holes. On the other hand, in the m a j o r i t y of cases b a s e or bottom fertilization can be avoided. In fertirrigation, chemical balance variations during the different vegetative stages of the crop will be carried out according to the nutritional needs of the plant, supporting the results obtained through sap analysis. It should be kept in mind that in intensive pseudohydroponic crops, the immediate needs of plants are very important. Below are two tables showing examples of seasonal fertirrigation programs for tomato and bell pepper crops. DR. HÉCTOR BURGVEÑO Bibliography (1987). Influence de (liferents systèmes de culture sur l'alimentation hydrique et minerale de la tomate (Lycopersicon esculentum Mill.). These de docteur i n g e n i e u r . ENSAT, Toulouse, France, 286 p. MORARD, Contrôle de la nutrition et de la fertilisation des cultures légumières par l'analyse de sucs extraits des tissus conducteurs. SETC. C. R. Acad. Agric. de France, Octobre 1982. ROUCOLLE, BURGUENO, H . MORARD, P . ETKERHOAS ( 1 9 8 2 ) . P. et ROUCOLLE, A. (1983). Diagnostic de la nutrition et contrôle de la fertilisation de la tomate et du concombre par l'analyse des sucs extraits de "gourmands". PHM NFI 242, pp. 37-41. A. ( 1 9 8 2 ) . Projet de find 'études. ENSAT. 181 p. + Annexes. ( 1 9 7 5 ) . Contrôle de la nutrition minérale des plantes au moyen de l'analyse des extraits frais de tissus conducteurs. I N R A , Bordeaux, 1 4 3 p. ROUTCHENKO, W . 41 LA FERTIGACION Dl NOflSRE: FQRHULA: DENSIDAD: PRECIO: MITR06EN0: FOSFORO: POTASIO: AZUFRE: UU FECHAS FESTÌ6ACIDN ; : DEL AL 2Î-Ï-Î3 2. 3Ö-X-93 3; 14-XI-RÏ 4, 2-ÜI-93 29-N:--3 CM 7 - Ì - 9 4 7; ÍS-I-94 s, 25-1-94 9i lí-II-94 0; 1-111-54 . 29-Ï-53 ¡3-XI-93 ;! i-ÜN-93 .. 22-X1I-93I; 0-1-94 17-1-94 ,. ¡4-1-54 15-11-54 25-11-94 .; 18-111-94;: ttlt I U I 1 2 T lili 4 5 6 7 S 9 0 ¡oox 1001 100X ¡001 ¡OOX 100X ¡001 1002 502 501 UREA SFT ABÖN NH45G4 1 >.63 46 20.5 0 0 0 0 0 0 0Ï OS OX OX OX OX OX OI 50 X 502 OX 01 01 OI 02 OX OX OX OX OX CaNQ3 POLIFSF AC FOSF «IT POT KNÖ3 H3P04 1 1.3? 1.6 1 «1.30 $3.00 »2.15 10.5 10 0 13 0 34 85 0 0 0 0 46 0 1? 0 0 OX OX OX OX OX OI OX OX OX 02 OX OI OI OX OXOX OX OX OX OX 100X 1001 100X 1001 1001 1001 1001 1001 1002 1001 OX 100X ¡OOX 1002 100X 702 701 50X OX OX 1 1 II till III u u tut VALLE DE CULIACAN, SIN. 7 CULT IVO: 0MATE DIVING FECHA DE TRANSPLANTE : 20-25-1-93 USAR-) NIT AH0N N03NH4 1 $.62 33.5 0 0 0 N ESUÎLISRIO : APL. ¡FERTIÓACION: KILOGRAMOS DE FERTILIZAWES/HECTAREA/DIA p K ..DIAS; ; g / o i i 3 3 . 5 - 0 - 0 46-0-0 20.5-0-0 10.5-0-0 10-34-0 0-85-0 : 1.00 1.00 1.00 i. 00 1.00 :. 00 1,00 i . 00 i.00 -. z 0. 1.50 .10; 1.30 .40, :.¡0 .60! .50 .80 .80 1.501 .70 1.60, .70 2,501 .60 2.80 ; .50 2.001 : • .4, 5 t 20; 9¡ ; UI 7, 22; ¡31 ¡81 ¡ ; i , Ï 1.4 1.4 ; I I ! i:6 i.3 Ì 1.1 3.11 4.79 5.83 6.03 5.00 5.97 5,57 5.05 3.01 3.12 3.06 3.50 3.37 3.15 3.26 2.68 2.39 2.15 1.42 ¡.23 2. ¡9 2.27 3-0-46 0-0 .36 1.91 3.17 a. 02 6.50 7.94 7.10 2 2 5 9 6 127 ¡UNIDADES FERTILIZ. Kg/Ha/ ; N P2Û5 K20 i; II I! i! II lì l; ;1 1.04 1.65 2.20 2.43 3.46 2.85 2.90 2.61 2.02 2.09 2.60 2.97 2.56 2.67 2.77 2.25 2.03 1.83 1.21 1.04 1 2 4 5 6 5 4 TOTAL UNIDADES FERTILIZ. 12,343 PLANTAS POR HECT/ 42 MATE if .8 50 0 0 0 96 . I ¡FERTI6ACIQN KILOGRAMS DE FERTILIZflKTES/HECTflREfl/PERÎODO H I ! 33.5-0-0 46-0-0 20.5-0-0 5 5 - 0 - 0 10-34-0 0-86-0 ¡3-0-46 7!! Ì4I! li! ss;: 2811 52; i 5!! 15! 1 8; i 15.55 67.03 46.63 120.55 72.01 65.72 39.02 111.05 39.16 56.12 28.52 40.87 632.85 (392 69.39 to 15.32 48.93 26.94 62.93 29.34 29.46 16.72 47.37 18.52 22.12 »0 »0 »0 d i ' UN IDA0E3 FEST I L I Z . Kq/Ha/PERIÖDO ITI! N R205 K20 TOTAL i ; Kg/fia/F i5i• '9ii '4!i ¡711 «¡1 ¡91 i 5U 8;! Mi! 1;! li ,! ¡: Ü I; I; . 11 ; Ü 0- 5.21 23.11 17.61 48.63 31.17 31.31 20.30 57.52 26.24 37.60 258.70 13.02 .00 41.59 2.31 22.90 7.05 53.49 29.18 24.94 24.94 25.04 46.94 ¡ 4 . 2 1 36.55 40.27 143.80 ¡5.74 73.47 ¡8.80 75.20 270.01 439.45 317.65 «953 5.02 ¡5.32 63.43 54.21 71.46 55.61 156.31 421.3c »906 TOTAL Kg/Ha/P 0-0-60 ; i 1 23.48; 18.271 ¡19.84, 122.44; ¡25.34! 409.37 «287 30.5e ¡20.99 88.89 246.91 155.56 190.¡2 129.63 434.57 208.64 244,44 1,850.62 »2.536 ¡8.23!i 67.01;, 47.56; ! 131.30, 81.04;; 103.31;; 71.06; 241,5911 115.45,; 131.60; I 1.008.15 43 LA FERTIGACIOî» tut tut un Ut) itti Itti tilt V A L l E DE OlifiCM. SIN. CULTIVO: E E L l FE-FE* VERDE. FECHA DE TSANSFLANTE: 27-25-1-93 ; FECHA ¡DEL AL NÍT AHON N03NH4 1 i.62 33.5 0 0 0 NÛHSRE: FORHÚLA: DENSIDAD: PRECIO: NITROSE«): FOSFORO: POTASIO: AZUFRE: 1 2 3 4 5 Uli 6 7 e 9 0 USAR==> litt ¡001 100Ï 10ÖZ 100! 100Z 100Z ¡OOZ ¡OOZ 100Í ¡OOZ OREA SFT AHON AC NIT POLIFSF AC F0SF NH4304 H3PÜ4 HNÛ3 1 1.3 1.39 1.6 1 $3.00 4O 20.5 55 0 10 0 0 0 34 85 0 0 0 0 0 0 0 0 0 0 OZ OZ 0! Oí 0Ï ¡II OÏ os 0Ï Oí oz Oí 0! OZ OZ OZ oz Oí oz oz oz oz oz oz oz oz oz oz oz oz OZ OÍ OZ oz Oí Oí OÍ 0! Oí Oí 100Z 100Z ¡ooz ¡ooz ¡ooz ¡ooz ¡ooz 100Z 100Z 100Z EQUILIBRIO ::EI'AD,: »FL. ;FERTI6ACI0K KIL06RAH05 DE FERTÍLIZAKTE / HECTAREA / ,1 ? K ; DIAS.! q/OL 33.5-0-0 16-0-0 20.5-0-0 55-0-0 10-34-0 0-85-0 i ¡30-K-ÍI 14-JI-93 ! 2.5 0. 2, IÎS-JL-93 21-.Ü-93 , 1.00 2.00 .00! r • ll-ñ-IZ T-«:-93 . I.èO 30! 4 ,£-«11-93 23-ÍÜ-93, 1.00 1.00 .40! ..00 .80 .80, 5 ,:4-¡¡;L-=3 6-1-94 1/-I-94 , 1.00 .80 .90; - ; ,7-1-94 7: -18-1-94 28-Ì-94 • ¡.00 .30 .sv; ,29-1-94 7-11-94 , ¡.00 .90 1.00! i ä - i i - 9 4 . 1.00 .60 .80! : i ,8-11-94 10-111-94, 1.00 .60 .60! 0 i; ¡ 9 - I ; - 9 4 ,, ,.uii 7:; lo,, 16,, ¡4!, Ili! tlü 10!! :li¡ 20.il -! • i. ! .3 .6 ! .4 .6 ¡ .7 ; .6 i .8 ! ! .3 ! 3.30 5.50 ¡0.20 7.39 9.10 9,96 3.25 4.33 7.03 3.28 3.71 4.21 9.55 ¡2.12 8.87 6.25 2.92 5.01 2.36 1.48 ¡31 UNIDADES FEST N P 1.11 1.84 3.73 2.79 3.94 4.48 4.13 4.73 3.35 2.10 TOTAL UNIDAI 32,757 PLANT 44 BELL PEPPER IR FOT KCL AC SULF H2S04 1 i.e ». 60 «.SO 0 0 0 0 60 0 0 96 0Ï 0Í Ol Ol Ol Ol Ol 501 501 1001 1 TOTAL Í IFERTISftCION KILOGRAMOS DE FERTILIZANTES / HECTAREA / PERIODO. ; TOTAL H l - 6 0 1 Kg/Ha/d! 1 33.5-0-0 44-0-0 20.5-0-0 55-0-0 10-34-0 0 - 8 5 - 0 13-0-4« 0 - 0 - 6 0 1 Í Kg/Ha/P 1 1 1 1 3.941 2.231 2.101 6.551 1 9,831 19.661 I 13.101 19.661 22.931 19.661 26.211 16.361 9.831 49.50 38,47 163.14 118.27 127.39 109.59 105.04 121,21 97.56 125.08 1,055.26 »654 48.78 30.32 112.41 52.55 51.90 46.34 32.10 50.07 26.01 29.58 »0 »0 ú / D I A ! K g / H a / d ! UNIDADES FERTILIZ. Kq/Ha/PERIODO (20 ! 1). FEST! N P205 K20 3.871 5.52; 10.82; 6.70! 19.241 12,08! 9.92Í 13.71! 8.04! 4.61! 16.58 12.89 59.72 44.67 55.14 49.23 45.47 47.29 36.85 41.50 41.46 25.78 95.55 44.67 44.11 39.39 27.26 42.56 22.11 25. I i .00 .00 17.91 17.87 44.11 44.31 36.38 47.29 29.48 25.14 LIZ./HA./CICLO- 409.74 408.04 262.49 .001 .001 1.12! 1.12! 3.15! 4.03; 3.31; 4.731 2.65! 1.26; iCTAREA »0 »0 TOTAL ! Kg/Ha/P ! 58.04; 38.66! 173.18; 107,211 143.37! 132.93; 109.131 137.13! 88.441 92.18! 1,080.27 480.05 »1,440 1 38.95 38.84 95.90 96.33 79.08 51.40 32.04 1 1 1 ; 1 39.41; 1 24.571 ; 41.901 1 432.53 105.57 I93C »64 98.28 68.80 314.50 205.66 275.1b 252.25 216.22 262.08 180.18 196.56 2,073.71 »3,086 Cultural vector control of virus: plant louse and white fly M . C . OCTAVIO POZO CAMPODÓNICO Instituto Nacional de Investigaciones Forestales y Agropecuarias M. Introduction I n the past few years, viral disease has become the most serious problem in horticulture production in Mexico, since it reduces yield and quality, principally in solanaceous crops (peppers, tomatoes, etc.) and cucurbitaceae (melon, cucumber, squash, watermelon) with a range of infection from 20 to 100%. Damage depends upon the severity of the virus involved, the phenological stage of the crop, and the speed at which the epidemic spreads. These crops become infected with several virus through various vectors. For example, pepper crops have registered the presence of (TEV) Tobacco Spot Virus, Cucumber Mosaic Virus (CMV), Tobacco Mosaic Virus (TMV), Tobacco Ring Spot Virus (TRSV), Potato Y Virus (VYP), the geminivirus complex which causes a disease known as Pepper Yellow Curl (RACH), and is also linked to Pepper Mild Tigre Virus (PMTV), Serrano Golden Virus and Pepper Huasteco Virus (PHV). Tomato crops have also registered the d i s e a s e s m e n t i o n e d above, and additionally Tomato Bush Shrinking (TBSV), Tomato P e r m a n e n t Disease (EPT), Tomato Spotted Wilting (TSWV), etc. 6 ' 10 ' 11 C. OCTAVIO Pozo CAMPODONICO Vectors registered as transmitters of one or several of these viruses are, in the first place, aphids. Myzus persicae and Aphis gossypii are outstandingly efficient transmitters, followed by harvest flies, trips, Paratrioza cockerelli, a psyllid, and the now famous white fly, most efficiently represented by Bemisia tabaci. Symptoms caused by diseases transmitted by these i n s e c t s a r e v a r i e d a n d have been meticulously described for each crop. However, in spite of the knowledge generated by the identification of vectors, their transmission methods, host range and an understanding of the etiology and epidemiology of the disease, problems have become more severe since the consignment of the first viral disease in 1971. By the mid-eighties these problems had become critical in some regions, while the virus transmitted by the white fly appeared in the early nineties, causing a 40 to 80% damage among the 70 thousand ha of peppers and tomatoes infected. 10 Viral transmission On their own, viruses do not penetrate their hosts. They do so through wounds caused by m e c h a n i c a l d a m a g e to developing root tissue inside the soil, or due to leaves brushing against each other CULTUM^IECTOI^ONTRO^^VINIS^LANHOUS^N^VHIT^ with the wind. This process is also carried out very efficiently by another organism which introduces viral particles from a sick plant to a healthy one. These organisms are called vectors, and are generally insects, nematodes or fungus. Many viruses are also transmitted through seeds, pollen and parts of plants used in vegetative propagation such as cuttings, rhizomes, guides or stolons and bulbs. 7 ' 9 The virus as a parasite is only active during the replication phase inside the living cell. The result of this replication is a great number of viral particles which are stored in inactive state in several parts of the cell. These become activated again when transmitted to other plants, or other parts of the plant. Under these circumstances, it is clear that virus survival depends almost exclusively on the survival and prosperity of its host. It is important to differentiate, ecologically speaking, these two transmission categories: viruses dispersed independently from the plants through a vector and those dispersed through plant parts. The exact underst a n d i n g of these viral transmission mechanisms, and latter development of the disease are important to design future prevention and control measures (9). Virus vectors In Mexico vectors with the greatest economical significance are transmitted by insects. The most notorious of which are aphids and white flies that transmit several kinds of viruses. Aphids. More than 190 species of disease-transmitting aphids have been identified, but the most common are: Aphis, Myzus, Brevicoryne, Rephalosiphum, Macrosiphum and Toxoptera, which altogether t r a n s m i t more t h a n 160 different viruses, most of which cause 50 mosaic and yellow symptoms. 7 Most aphids transmit non-persistent type viruses. In this case, the insect acquires the virus while feeding. The virus isn't swallowed but carried in the oral apparatus (probe) and retained only a few hours. Inoculation also takes place in a matter of hours (maybe a few seconds or minutes). Non-persistent viruses can be transmitted through the sap and have a wide range of hosts. In Mexico nonpersistent viruses transmitted by aphids are the Cucumber Mosaic Virus (CMV), Tobacco Spot Virus (TEV) and Potato Y Virus (PVY) among others, which can be mechanically transmitted. 11 White fly (Bemisia tabaci). At present, this is the most important and widely distributed vector in the country. It generally causes yellowing, foliar curling and some mosaics. Initially, these viruses and vectors probably could only be found in tropical and subtropical areas, from where they have since spread to other ecological environments with a great capacity to adapt and form new biotypes. In general, the white fly transmits v i r u s e s of t h e s e m i - p e r s i s t e n t and persistent category. An acquisition period of 24 to 48 hours is enough to render viruses ineffective. The virus observes a latent period inside the body of the insect which lasts from four to twenty hours. It can be acquired by the nymphs, persist during pupation and be transmitted by the newly emerged adult. 7 The virus is carried the insect's hemolymph, which can be swept away by the wind and thus transmitted over great distances. White flies prefer to feed on young tissue and on the right side of leaves. They transmit the geminivirus complex which causes the disease known as Pepper Yellow Curl (RACH) formed by Tomato Curl (CdTV) and Pepper Mild Tigre Virus M. (PMTV), Serrano Golden Virus (SGV) and Pepper Huasteco Virus (PHV) which are not mechanically transmitted. 11 Dispersal patterns Distribution patterns for the disease in the field provide valuable information to identify the type of vector involved. For example, if after the initial presence of the disease, the infected area remains static and doesn't spread, it means that the virus is probably in the soil, in which case, the vector would be a fungus or nematode. On the other hand if initial presence of the disease is in individual plants evenly distributed within the field, the dispersal C. OCTAVIO Pozo CAMPODONICO agent is likely associated to be associated to the seed.9 The primary stage of an epidemic caused by aphid-transmitted viruses mainly involves solitary plants which invariably can be found on the edges or borders of the plot. The disease progresses inside the field through solitary plants inoculated one by one from the areas first infected. A similar situation can be observed in viruses transmitted by the white fly, except for the initial infection period, which can occur in the presence of groups of two or three plants. Transmission to other plants along the row can be expected according to the period the fly retains the virus. Table 1 Disease dispersal pattern and probable vector* Dispersal pattern Probable vector Restrained, static initial infection, infected plants are grouped. The vector is in the soil: can be fungus of nematodes. Uniform distribution of isolated infected plants. Seed of acarus infection. Isolated plants restricted to the edges of the plot. Aerial vector, possibly aphids. Groups on infected plants and linear dissemination. Aerial vector, possibly white fly. Secondary dissemination. * Taken from Nelson, M. R. 1991. Control of viral disease Contrary to fungus and bacteria, viruses can't be controlled with chemical agents. There are c e r t a i n antiviral compounds under development, but high costs, high phytotoxicity and legal considerations have impeded t h e i r commercial use. Therefore indirect control measures, such as genetic resistance and vector control represent the only practical means to control the virus. In the course of research on viral disease, the greatest success has been achieved in the understanding of the structure and composition of virus. These Cultural vector control of virus: plant louse and white fly studies were very well supported by the most advanced technology such as the electronic microscope, ultracentrifugal units, spectrophotometers and electrop h o r e s i s t e c h n i q u e s , serology and molecular biology methods. This knowledge served to lay down the framework for a control strategy against viral disease, and its main contribution has been diagnosis processes in which its necessary to correctly identify the problem viruses and have a good understanding of their epidemiology and ecology.9 A description of some of the most common control methods follows. A. Genetic resistance Cultivars genetically resistant to viruses and/or vectors represent the most effective strategy to control viral disease, since they keep viruses from penetrating plant tissue or avoid their multiplication and movement inside the plant. However, since crops are infected by a virus complex and possibly by many varieties with the help of one or more vectors, it is extremely difficult to obtain good results with traditional genetic improvement methods. Save for some relatively isolated cases, success with this method has been very poor. It can be said that improved resistance to the vector is what should be sought, since a genera or species like aphids, and particularly Myzus persicae, are responsible for the transmission of several types of geminivirus. There is hope t h a t biotechnology and current development of transgenic plants will in the future serve a practical application. 11 52 B. Cultural control Even though cultural control of viral disease is a very broad concept, as far as we're concerned, it refers to integrated vector management, including a series of practices tending to minimize or avoid vector activity inside the crop. In the development of integrated control of viral disease, the greatest success has been met by broad management or control propositions such as production of virus-free m a t e r i a l s (seeds, stolons, cuttings, tubers, rhizomes, plants, etc.) and the control of alternating hosts of the virus and the vector. Some experimentally proven practices, validated by growers, are described below. 8 ' 9 Plastic mulch Both aphids and white flies are sensitive to reflecting sunlight. This effect can be achieved with reflecting tapes or plastic mulch, which make it difficult for these insects to approach the crop, and in the case of vectors, decreases the incidence of viral transmission. Plastic mulches have been used for other purposes: water use optimization, weed control, temperature conservation, etc. However, according to experimental d a t a and t h e experiences of m a n y producers and technicians, mulches offer the lowest percentages of viral infection as can be seen in Table 2, where plastic mulch shows a clear advantage affording good control u n d e r h i g h infection conditions. 12 M. C. OCTAVIO POZO CAMPODONICO Table 2 Use of plastic mulch to control viral disease in pepper. INIFAP, Percent of diseased plants Weekly sampling 4* 3* 2* 1* 13.10 0.00 0.00 1 0.02 59.58 5.20 12.50 2 17.29 16.16 72.10 23.54 31.25 3 36.00 86.60 31.20 39.50 4 92.90 100.00 89.50 90.20 5 1988* 5* 3.33 9.58 13.10 17.90 24.10 * 1. Black plastic. 2. White plastic. 3. Aluminum plastic. 4. Untreated witness. 5. Intensive chemical control. ** Taken from First International Congress on New Agricultural Technologies. Manzanillo, Colima, Mexico, 1993. On the other hand, while evaluating the efficiency of black plastic mulch over Myzus persicae population dynamics in melon crops in southern Tamaulipas, and the effect of the latter upon yield (Figure 1, Table 3), it can be seen that aphid populations appeared in the mulched plot during the sixth week after transplanting. Virosis infection was noted on the seventh week, and coincided with melon plants spreading over the mulch, thus impeding its reflecting action. In the unmulched witness plot and the plot with chemical control only, plant lice populations were detected during the second week; disease set in during the fourth week and registered 100% infection by the ninth week.2 As far as production, the protection afforded by plastic mulch is evident, since export fruit yield was 300% greater than that of the witness, and represented a benefit-cost ratio of 1:2 according to existing prices and production costs at the time. Table 3 Treatment Mulched Unmulched Melon yielc (boxes/ha) National Export 515 330 174 116 Yellow traps Over the past few years, the use of yellow glue t r a p s has increased in integrated virus management programs. Other 298 154 Cultural vector control of virus: plant louse and white fly FIGURE 1. PERCENT OF V1ROSIS AND VECTOR POPULATIONS IN TREATMENTS WITH AND WITHOUT PLASTIC MULCHES. 54 M. The fact is that various insects, including aphids and white flies, respond positively to the action of the wave length reflecting yellow (500-700 nm). This has been used as a control measure against these insects to decrease the propagation of viral disease in horticulture crops. Data from experiments carried out in southern Tamaulipas (INIFAP) clearly C. OCTAVIO Pozo CAMPODÓNICO demonstrate t h e advantages of this practice (Table 4). After 14 weeks of observation, treatment with yellow traps plus chemical control showed 19% of sick plants; treatment with yellow traps only registered 33.5% and the witness, 87.5%. Likewise the number of adult white flies captured by sampling was significantly lower in the yellow trap plot. Table 4 Effect of yellow traps on adult Bemisia tabaci. Percentages of diseased plants and fruit yield. CESTAM-INIFAP, 1988 Treatment Yellow traps + chemical control Yellow traps Witness % virosis Average no. of white flies Yield (two harvests) kg/ha 19.0 3.2 a 3,062 a 33.5 87.5 3.5 a 6.3 b 2,879 a 535 b On the other hand, while evaluating yellow traps in contrast to chemical control, the former proved to be statistically equal to the best insecticides (Table 5). 55 Cultural vector control of virus: plant louse and white fly Table 5 Effect of yellow traps and different insecticides on adult Bemisia tabaci. CESTAM-INIFAP, 1989 Treatment Permetrine Lamdacialotrine Cipermetrine Yellows traps Fosfamidon Endosulfan Witnesses Average NQ of white flies Yield kg/ha 2.3 ab 2.9 abc 3.5 abc 11,647 a 11,669 a 3.6 abc 3.8 be 3.8 be 4.7 c Polypropylene cloth and plastic net covers (floating covers, tunnels etc.). In some areas of the country, crops are covered with synthetic cloth to avoid contact between plants and vims vectors. This technology is rapidly spreading. In Mexico, the first experimental evidence was gathered by INIFAP-CNPH in melon crops in t h e valley of Apatzingan, Michoacán. At present there is more experience in crop management with plastic covers and several materials for this purpose are available in the market. Studies carried out by the Postgraduate College in the states of Morelos and Sinaloa in squash and tomato crops shed more light on the advantages of this technique. 1 Likewise, in Yucatán where viral damage to tomato crops was over 50% with more than a 70% reduction in yield it was possible to live economically with the problem by developing an integrated strategy for vector control which includes, a m o n g o t h e r practices, p l a n t u l e production in nurseries covered with polypropylene cloth.4 And so, all over the country covers in different modalities 56 10,768 a 7,346 a 9,165 a 8,438 a 3,632 b (floating, micro-tunnels, sheds etc.) are in relative use. This technology, however, has great disadvantages which limit its use in different producing regions. Among these is the short protection period, since most crops must be uncovered when blooming starts due to floral abscission generated by adverse environmental conditions inside the tunnel or floating cover. Another disadvantage is excessive vegetative development, principally in tropical regions where the quality of solar radiation is poor. Inside tunnels or floating covers plants don't receive adequate light so their vegetative development isn't normal: they grow long internodes which make weak stems. On the other hand, high temperatures and relative humidity inside the structure afford an ideal environment for fungus and bacteria infections. Plastic screen barriers. One of new alternatives to correct the disadvantages of floating covers and tunnels described above, so as to better benefit from the comparative advantages they offer, is to use antivirus or antivector screens as M. barriers around the crop. Synthetic barriers measuring 2 m high minimize insect access and serve as windbreakers with the added advantage that normal cultivation labor can be carried out in the plot u n d e r n o r m a l e n v i r o n m e n t a l conditions of light, t e m p e r a t u r e and relative humidity. According to e x p e r i m e n t a l obser- C. OCTAVIO POZO CAMPODONICO vations made in southern Tamaulipas by INIFAPto determine population dynamics of white flies through traps set at different heights, it was found that between 60 and 70% of the population was captured at an altitude of less than one meter; 85 to 92% was caught between 1 and 1.5 m., while only 8 to 14% were found above 2 m (Table 6). Table 6 Population dynamics of white flies at different altitudes. INIFAP, 1994 Height of the traps (cm) % of white fly* Height of the traps (cm) % of white fly** 70 33 30 32 100 29 60 25 150 24 90 14 200 14 120 12 150 9 200 8 * Data furnished by M. C. Joel Avila Valdez from six weeks of sampling carried out in 1987. ** Data gathered by the autor after six weeks of sampling in 1993. With this information, plastic screen barrier efficiency over white fly population dynamics in serrano pepper crops was evaluated. In a highly infected plot, a 300 m2 (30 x 10 m) area was isolated with antivirus plastic screens. Every day the population dynamics of the insect were determined both inside and outside the barrier through visor counts. Three days before starting these counts, the white fly population which remained inside the barriers was controlled with Permetrine and Endosulfan. Results are shown in Table 7, where the advantages of synthetic barriers to control vectors are evident. Cultural vector control of virus: plant louse and white fly Table 7 Population dynamics of white fly inside and outside plastic barriers. INIFAP, 1994 NQ of days White fly population White fly population on the outside* in the inside* Observation Chemical control 1 3 86 2 4 82 2 5 8 0 6 12 0 7 32 1 11 127 2 12 133 6 13 137 10 14 110 9 19 197 46 20 199 61 21 126 4 22 8 1 North North Chemical control North * Ten visor strokes per sampling. We have p r e s e n t e d h e r e some experiences which may contribute to minimize the effects of vector insects. Unfortunately a successful solution to the problem isn't easy and generally requires multiple strategies. These must take into account ecological conditions prevailing in the region, an understanding of the 58 viruses involved in order of importance, a good understanding of the epidemiology and adequate knowledge of the biology and ecology of the vectors. With all this information, experts in integrated pest management (IPM) can design a control program adequate for each region. M. C. OCTAVIO POZO CAMPODONICO Bibliography 1 2 3 AVILA, V . J . y Pozo, C. 0 . 1 9 9 1 . Establecimiento de una parcela de validación para el control de enfermedades virales en melón. Memorias IV Congreso Nacional SOMECH. Saltillo, Coahuila, p. 204. DÍAZ PLAZA, R . y RAMÍREZ CHOZA, J . 8 GREEN, S. K. and KIM, J . S. 1991. Characteristics and control of viruses infecting peppers: a literature review. AVRDC. Tech. Bull. N 2 18, 60 p. GARZÓN, T . J . A . , BUJANOS, M . R . y BYERLY, Presencia de virus en los cultivos de chile Capsicum annuum L.) y tomate (Lycopersicom GARZÓN, T . J . A. 1987. M. R. 1991. Plant virus management. Trabajo presentado en el diplomado "Administración y Eficiencia en la Producción de Hortalizas". ITESM-Campus Querétaro. 9 NELSON, 10 Pozo, C. O. 1991. Estudio y control de las enfermedades virales en el cultivo de chile. Trabajo presentado en el diplomado "Administración y Eficiencia en la Producción de Hortalizas". ITESM-Campus Querétaro. 11 Pozo, C. O. 1993. Control de virosis en el cultivo de chile. Minisimposio II. Manejo integrado de virosis en cultivos hortícolas. V Congreso Nacional de Horticultura. SOMECH-Veracruz. 12 Pozo, C. O. 1993. ler. Congreso Internacional de Nuevas Tecnologías Agrícolas. Manzanillo, Colima, México. M. K. F. 1992. Manejo integrado de la enfermedad "permanente" del tomate (Lycopersicom lycopersicum L.) en el Bajío. Informe de Investigación C A E B . 6 G R E E N , S . K . 1 9 9 1 . Guidelines for diagnostic work in plant virology. AVRDC. Technical Bulletin N2 15, 63 p., second edition. L. 1991. Bioecología y control integrado de la mosquita blanca Bemisia tabaci Genn. (Homoptera: Aleyrodidae). Campo Experimental Zona Henequenera. C I R S E - I N I F A P . Publicación Especial, número 3. 5 7 AVILA, V . J . y WOLFENBARGER, D . A . 1 9 9 4 . Yellow trap and insecticide for control of white fly and virus incidence on pepper. In press. J . Entomological Sciences. 4 esculentum Mili) en México. Informe de Investigación CAEB-INIFAP. Tomos de Virología II. Sociedad Mexicana de Fitopatología, pp. 156-18. ACOSTA, L . P . , RODRÍGUEZ, M . R . y G u z - MÁN, R. P. 1991. Epidemiología del chino del jitomate y su control mediante cubiertas flotantes en Morelos. Memorias XVII Congreso Nacional de Fitopatología. Puebla, Puebla. México, 70. Improvement and control of indoor horticulture D R . J O S É LÓPEZ-GÀLVEZ "Las P a l m e r i l l a s " E x p e r i m e n t a l S t a t i o n DR. Contents 1. 2. 2.1. 2.1.1. 2.1.3. 2.1.4. 2.2. 2.3. 2.3.1. 2.3.2. 3. 3.1. 3.2. 3.3. 3.4. 4. 4.1. 4.2. 4.2.1. 4.2.2. 5. 6. Introduction Greenhouse agriculture in Almería The physical environment Climate Water Soil Transformation background Present situation Technical aspects Economical aspects Technological breakthroughs in Almeria-type greenhouses Plastic materials for closures Microclimate Water management techniques in crops Economical results Micro-climate improvement in low-cost greenhouses Techniques for temperature improvement Techniques for radiation improvement Closure materials Orientation and shape of the cover Conclusion Bibliography JOSÉ LÚPEZ-GÁLVEZ DR. JOSÉ 1. Introduction T h e concept of a g r i c u l t u r e h a s traditionally been understood to pertain to activities strongly dependent upon the natural physical environment. It's not surprising, therefore, that the agricultural prosperity of a place was conceived as something related to favorable soil, climate and water conditions. Any unfavorable circumstance with regards to any of these factors would limit farming potential to a degree that could result in its losing all economical interest. The aforementioned limitations can be helped through various technological innovations. This is the case of crops growing on artificially created media. Thus, the development ofhydroponic crops and crops growing on substratum makes it necessary to re-evaluate the traditional role of the soil. On the other hand, the use of greenhouses also modifies environmental variables totally or partially. It's reasonable to assume that, under such controlled growing conditions, the limiting factor would be water; but in this respect also, notable achievements have been made during the past thirty years with localized irrigation systems. All these important transformations have been made possible, to a great extent, with the LÛPEZ-GALVBZ appearance of plastic materials. It's hard to imagine our modern day world without plastics. The general value of this idea (as can readily be seen in the generalized use of plastics for manufacturing all those things that make our lives easier) can be faithfully applied in the area of agriculture, which has thus benefited from a true revolution. These m a t e r i a l s not only improve w a t e r management (with distribution networks, regulating deposits, irrigation systems and drainage networks) but also allow us to alter climate conditions in the environment with mulches, small tunnels, protection screens and even greenhouse roofs. Mexican agriculture is no exception to the use of plastics to optimize irrigation water consumption and to control or alter climate (Reyes, 1992). The use of passive climate g r e e n h o u s e s allows tomato production during the months of May and June (transplanted in January) in the state of Coahuila (Ibarra and Quesada, 1992). Borrego and Co. (1992) show how the nopalito (Opuntia ficusindica) is produced in greenhouses with satisfactory economical returns in areas near the border with the United States. Experiences with greenhouses in different parts of Mexico promise better productivity, which makes greenhouses all the more interesting Improvement and control of indoor horticulture within the framework brought about by t h e N o r t h A m e r i c a n Free T r a d e Agreement, NAFTA, signed by this country, Canada and the United States. These new circumstances undoubtedly reinforce the competitiveness of Mexican agriculture, through the adequate use of its advanta-geous climate to obtain firstrate crops, and even expand its productive calendar. One of the greatest examples in intensive in-doors production techniques, with respect to European competitive terms, is in Almería. I assume that the experience brought about by its "sandy" cultivation system, proper to the Almería coast, as well as research done on the subject under passive climate greenhouses, makes it possible to draw particularly useful knowledge for the development of agriculture in other areas with moderate temperatures. These local technologies have brought together artificial soil preparation, localized irrigation and the establishment of simple greenhouses, with low investment costs. These create a favorable micro-climate which can increase yield and crop precocity, which make them competitive against more sophisticated and expensive greenhouse techniques developed elsewhere. I wish to reflect on the potential of greenhouses referenced in moderate climate areas. I'll describe Almería 66 techniques used to improve natural environmental conditions. I'll try to give some orientation on the design of the buildings used. In short, the idea is to share an experience which can prove useful under Mexico's agroclimatic conditions, which are better than those of its partners in NAFTA. 2. Greenhouse agriculture in Almería 2.1. The physical environment The province of Almería lies in southeastern Spain. 90% of its greenhouses are concentrated in an area west of the capital city. This area limits to the north with the Gador Sierra and to the south, with the Mediterranean; it's located between 36941' and 36s49' latitude North and 2S33' and 2S54' longitude West. Since its inception around 1965, greenhouse cropping has grown significantly inside this strip of coast. 2.1.1. Climate This information, from the 1982/1983 to 1991/1992 campaigns, was taken from the 155 m high automatic meteorological installation of the "Las Palmerillas" Experimental Station, located on 36a48' l a t i t u d e North, and 2 e 43' longitude West. DR. JOSÉ LÓPEZ-GÀLVEZ Air temperature Minimum absolute temperatures are over (FC. January and February are the coldest months. Maximum absolute temperatures are over 309C during the months between June and November. O N F M A M MONTH MAX. M. 24h M . DAY ABS. MIN. ABS. MAX. MIN. M. NIGHT Relative humidity KM. Minimum humidity values under 20% are common. Practically every month of the year includes days with values under 30%. Each month there are moments close to saturation. O N D —e— J F M A M MONTH •— 1 MAX. M . 24h M . DAY ABS. MIN. ABS. MAX. MIN. J J A S —«— M . NIGHT 67 Improvement and control oj Evaporation Evaporation in a class "A" tank was: Campaign 1982-1983 to 1991-1992 Yearly average mm / day 5,1 Yearly average mm 1 day 17,0 Yearly total mm 1.894,3 Wind W i n d s a r e p r e d o m i n a n t l y westsouthwest and from the East. Breezes are more frequent during summer nights. Maximum gust was 112 km/h, blowing 120s Southwest. MONTH MAX. GUST 24 h. GUST DAY GUST K\Ml/m2 DAY NIGHT GUST Global solar radiation Mean monthly values are lower than 5 kWh/m2. day through the months of November-J anuary. MONTH 68 DR. JOSÉ LÚPEZ-GÁLVEZ Rain Scarce; annual average is lower than 250 mm. Campaigns 1976-77 to 1990-1991 Greatest drought 84-85 Total rainfall (mm) 226,2 Máx. rainfall in a day (mm) 51,7 NBof rainy days (mm) 47,1 109,2 13,8 42,0 2.1.2. Water Poor rainfall and high superficial permeability of the soil don't allow continuous waterways of relevance. Subterranean waters are the most used. There are three water deposits in the area, and their contents, though of different qualities, are considered acceptable for present agricultural activities. 2.1.3. Soil The soil of the area is very heterogeneous. In general, though, its basic (saline and alkaline) soil, shallow and almost sterile. 2.2. Transformation background The physical description of the area gives it a certain desert quality. Historically, farming practices were seriously difficult and rather impossible given these extremely hostile edaphoclimatic characteristics, poor soils and extremely scant and irregular rainfall, in addition to scarce superficial water resources, strong and frequent winds, all of which didn't make adequate development viable. Technological innovations have made it possible to farm in this area, by changing old limitations into new advantages. These i n n o v a t i o n s w e r e geared t o w a r d s alleviating the main limiting factors (soil and water) and to get the most from the beneficial factors. Thus, relatively benign temperatures during the winter months and excellent insolation translate into improved crop precocity and productivity. Drainage systems are unnecessary given soil permeability. Even the winds have become a positive factor, since they eliminate the need for mechanical means of ventilation and the cost associated with the energy they consume, making greenhouse ventilation easy. The clue to technological advancement lies in advantageously relating water control (through irrigation) to soil control (with sandy soil) and environmental control (with plastic "arbor-type" greenhouses). The resulting system can be summed u p as irrigation agriculture developed upon sandy soil, in low-cost greenhouses not equipped with heating. This whole body of applied techniques is deemed "exportable technology". and control o) 2.3. Present situation growing (which is traditional in the area) made with wooden and wire. The framework is fixed on wood poles set upon concrete blocks. Wire is used to anchor the structure, tie the posts and form top and side screens. These support the closure material, which is flexible polyethylene, placed between two wire screens, then joined with short pieces of wire. Since roofs are usually flat or with only a slight inclination, they must be perforated to eliminate rain puddles. In the beginning, the greenhouses described above were lower than 2 m; now they can be higher than 2.5 m. This makes it easier to grow crops of indeterminate height, through training practices. All this results in a functional greenhouse which allows partial control of the microclimate, reduces évapotranspiration (ET) in the crop, curbs wind damage and slightly improves the thermal regime. 2.3.1. Technical aspects The irrigation system used isn't the most outstanding feature of the cropping technology being described. Even though drip irrigation systems are the most common, flood irrigation is still applied in almost a third of the land irrigated. What's truly characteristic are the soil preparation techniques and the type of greenhouse utilized. The sand preparation technique was implemented in the fifties. It includes initial plowing and leveling, as well as the addition of a 20 cm layer of vegetable soil, mixed with 5 kg/m2 of manure. Another 2 cm layer of manure is applied next, which is then covered with a 10 cm layer of beach sand. All together, this makes four clearly distinct strata, both in their physical as well as their chemical structures. Their positive effects last two to five years, after which it becomes advisable to redo the soil by hoisting the sand layer to replace the manure. In the sixties, the sand cropping technique was augmented with greenhouse protection. A characteristic of these greenhouses is their simple, low-cost construction inspired by structures used in vine- 2.3.2. Economical aspects The application of the above mentioned techniques, adapted to the conditions of the area, has enabled the implementation of a very intense agriculture, one of the most profitable in Spain. The following table illustrates the spread of greenhouses throughout the province of Almería. HECTARES 26000 20000 160CO 1 10000 1 1 1 6000 0 i i i i i Mi i i i l l iHiflm 1965 70 68 71 76 79 81 i 83 86 i 90 DR. JOSÉ The economical importance of this agriculture, with regards to its share in the gross domestic product (GDP) and Sectors Agriculture (Greenhouse) Industry Construction Servicios Total GDP LÓPEZ-GÁLVEZ the demographical dynamics of Almeria has been highlighted by Naredo & Co. (1993). 1991 GDP by sectors GDP (%) Almería 18,7 (12,7) Spain 11,1 13,7 24,3 8,8 62,9 56,6 100,0 4,0 - 100,0 Source: BBV. National income and its distribution by provinces, 1991, and own data. The table shows how agriculture is much more important to the Almeria economy than to that of the whole of Spain. This is more on account of the dynamism of t h e region's a g r i c u l t u r e t h a n of stagnation in other sectors. When we add t h e goods a n d services r e l a t e d to greenhouse cropping, we find that the latter represents close to 70% of total agrarian production and accounts for more than 35 % of provincial GDP. When we compare t h e d i f f e r e n t territories, it can be seen that population growth between 1965 and 1991 was uneven. Coastal territories close to modern intensive agriculture and tourism showed substantial population growth. The rest, which includes traditional a g r a r i a n exploitations, showes decreased population. 71 and control of indoor horticulture Demographic dynamics of farming territories in the Almería province Population Territory Variation 1965 1991 124,1 192,2 54,9 Alto Almanzora 58,9 49,2 -16,5 Alto Andarax 24,7 16,1 -34,8 Bajo Almanzora 45,9 47,4 3,3 Campo de Dalias 64,7 117,0 80,8 Campo de Tabernas 21,3 12,2 -42,7 Los Vêlez 19,2 12,4 -35,4 Río Nacimiento 17,3 9,0 -48.0 376,1 455,5 21,1 C. de Níjar y B. Andarax Total province 1991/65 Source: INE. Compiled by Research Studies of the Chamber of Commerce of Almería. 3. Technological breakthroughs in Almeria-type greenhouses 3.1. Plastic materials for closures The system is only viable when there are contrasted quality plastics available atareasonable price. Jorge &Bretones (1990) describe the problems and the evolution of closure materials. Among the first (in the early seventies) major drawbacks of plastics, they point out the following: - Uneven film thickness. - Rapid aging and degradation. - Poor mechanical resistance. - Poor thermal protection under low temperatures. These authors add that the improvement and evolution of plastics was carried out efficiently, thanks to interdisciplinary cooperation between the industry and agrarian experimental centers. This resulted in long-lasting, thermal-insulating films in the marketplace. 3.2. Microclimate Greenhouse production results from the improved environments these structures afford plants, in terms of light, temperature, relative humidity and air composition. The main objective of industrial greenhouses is to bring optimum climate parameters closer to each plant species. Research carried out to date on the microclimate generated by the Almeria greenhouse, enables us to pinpoint the d i f f e r e n c e s b e t w e e n t h i s cropping technique and the one used in said industrial greenhouses. Given its lack of complicated means to modify its microclimate, temperatures and relative humidity in the Almeria-type g r e e n h o u s e a r e below a n d above, respectively, those prevailing outdoors during a good part of the night (LôpezGâlvez & Co., 1991). However, since the heat generated by the sun is better utili- DR. zed during the day, this greenhouse improves the temperature integral and shortens the period between the various phenological stages, thereby increasing crop precocity. Consequently, it should be noted that this crop system does not really fit the usual concepts of "greenhouse cropping" or "forced cropping"; nor do its installations act like true greenhouses, and crops are not "forced" with the introduction of strict environmental controls. In fact, this system focuses more on enhancing favorable natural conditions through simple and low cost materials, than on creating an artificial habitat apart from the natural hostile conditions. The main feature of the Almeria-type greenhouse worth mentioning however, is its low cost and easy construction, which doesn't require any specially trained labor. The fact that with an investment (purchase and preparation of the land, forming the sand bed, placing irrigation system and buildingthe greenhouse) lower than 20$/m2, this system achieves high yields from various crops, over 5$/m2, during an ample calender, only highlights its economical advantages, even though the microclimate it provides is far from the optimum required by the different garden vegetables presently grown under these conditions (Montero & Col. 1984; Lopez-Galvez & Bretones, 1988; LopezGal vez, 1991). This is well illustrated by the fact that during the years 1976-1984, average minimum temperature inside these greenhouses in January was 9.92C, and average maximum temperature in July was over 37aC. These readings are pretty far from what is considered optimum for many for the crops under exploitation. JOSÉ LÚPEZ-GÁLVEZ 3.3. Water management techniques in crops One of the principal objectives of the Almería irrigation system is to increase water consumption efficiency. Here irrigation management should focus on obtainingthe highest possible productions, avoiding hydric s t r e s s which could diminish yield or quality. This is especially important due to the scant capability of the soil to retain water, which explains the need to create an artificial soil (sand bed), build regulating deposits (which have spread throughout the crops) and implement drip irrigation systems. Drip irrigation allows the application of water and fertilizers with just the right frequency and on the base of the plant. Under this premise, the role of regulating water and nutrients reserve traditionally supplied by the soil are no longer essential and serves as mere support to the crop. In such a framework the only information necessary for good water management is knowing t h e c o n s u m p t i o n due to evapotranspiration (ET) and that pertaining to salt washing. This data, together with a knowledge of the yield of water application by evaluating the irrigation system, enables us to make reasonable decisions regarding the amount of water to be applied to the crop. Best water management in the Almería agrarian system has been based on the following: Estimate of the evapotranspiration of the principal crops. It is known that during previous experimentation, the precise dose of irrigation -Castilla & Co. (1986) and López-Gálvez & Co. (1990)- determined ET consumption of the crops using drainage lysimeters to obtain all the components of the hydric balance: Improvement and control of indoor horticulture ETc = R - L + AW With the following variables: ETc = évapotranspiration of the crop. R = w a t e r supplied by rain and irrigation. In our case, rain is nil. L = lixiviated water AW = variation of the water content of the soil. Under the conditions of the AW30 tests. For further experimentation there is a class "A" evaporimeter tank, normalized by the W.S. Weather Bureau. This tank was placed inside a greenhouse growing g r a s s (Lopez-Gâlvez & Co. 1988). Considering that evapotraspiration of the crop is measured by the expression [ETc = k Eo], where Eo is the evaporation in the tank and k is an adimensional coefficient encompassing the tank and crop coefficient -estimated [k = Etc/Eo]-, with data from several years of tank evaporation, it has been possible to write a series of index cards giving required water dosages for the different crops in periods of two weeks. Evaluation of irrigation systems and homologizing in the quality of the drip branches. This evaluation and homologizing is to confirm whether the irrigation systems are operating correctly. That is, whether they're applying and adequate amount of water on the crop occupied surface and if this amount is uniform. Due to potential risk of plugged-up drip holes, drip irrigation systems have to be evaluated frequently. A part of good water management is to check the system periodically. Caja Rural de Almeria ( 1985) promotes work on evaluation of the drip irrigation systems installed in greenhouse crops. Final conclusions and recommendations on work already carried out are the following: 1. The quality of the installations is acceptable. In general, the work done by 74 the companies in charge ofthe installations is positive. However, there are certain deficiencies in technical design which reflect upon the cost of the installations. These deficiencies could be easily corrected by adapting the power of the pump, filter and fertirrigation equipment to the true needs of the installations. 2. Uneven water application, taking into consideration that the systems under evaluation were recently installed. The basic reason for poor uniformity is deficient drip holes, a fundamental factor the installer should be considering. 3. Its alarming that almost 50% of the tubes evaluated were not manufactured with the right raw material. The quality is unacceptable both by the UNE 53367 standard and international tube standards. 4. The need for more precision in the diameters of the branches m u s t be stressed, in order to avoid value disparity and p r e f e r e n t i a l l y a d h e r e to those expressed in present standards. 5. Once installed, the system is operated without any sort of technical instructions as far as water volume or fertilizers to be used. These results showed the need for a guarantee document between farmers and drip irritation installers, in order to overcome the deficiencies stated. Additionally, a series of index cards was prepared providing the steps to be followed while evaluating irrigation systems. This was done by adapting the method proposed by FAO. These cards, together with average water consumption in the crops, contributed to the following: A. Drip irrigation systems. The degree of quality in the tubes has improved. A good part of the material used is made by a quality manufacturer. Also acceptable in most exploitations is the uniformity coefficient in the application of water DR. through the irrigation systems. (Losada & Co., research under way.) B. Water expense. Has been reduced from more than 7,000 m 3 /ha per year, accordingto measurements taken in 1982, to less than 5.500 m 3 /ha per year. Data pertains to areas irrigated with good quality w a t e r (López-Gálvez & Co., investigation under way.) An adequate water management program should consider other factors such as: Salt control. The distribution of salts in the profile of drip irrigated soil basically depends upon physical characteristics, (texture and structure, for example) besides other extrinsic circumstances, like emitter expense, application frequency, washing fraction and radical distribution. Total wash is particularly hard to achieve when using localized irrigation systems. At Almería, the technique consists in applying flood irrigation (30 to 40 mm) at the end of the campaign. No soil salinity problems of importance have surfaced, even when irrigating with water whose electrical conductivity is over 3 dS/m. However, in our opinion, orientation to the growers should be based upon more detailed experimentation on the movement patterns of water and salts in the soil. Fertilizer management. The possibility of applying fertilizers through the drip irrigation system has implicit potential advantages. Among these, a more precise and efficient application, since it is done JOSÉ LÓPEZ-GÁLVEZ by parts and directed to a specific place, which reduces losses through filtration and denitrification. The fertilizer management program should conform to the water management program. It's advisable to make frequent applications, trying to cover the needs of the crop. In this field, there have been many investigations carried out in Almería (Castilla 1986; Martínez, 1987; López-Gálvez, 1991.) 3.4. Economical results This section summarizes representative economical data coming from the i n f o r m a t i o n g a t h e r e d by t h e "Las Palmerillas" Experimental Station through a network of cooperating farms, as well as through its own experimental growing experiments. The data is from the 1990-1991 campaign. All monetary numbers in this document are given in US dollars, at the average exchange rate prevalent during the 1990-1991 campaign (100 pta = $1). Immobilized, fixed. Here we contemplate the purchase of the land, making the sand bed, building the greenhouse, the irrigation network and construction. Total investment was between 14 and 22 $/m2. Productions and income. The following table shows the values surrounding the yields, prices and income for crops and most common alternatives. 75 Improvement and control of indoor Annual alternative 1 horticulture Production and ncome from the crops Production Prices Crops kg/m2 $/kg Dutch cucumber 7-10 0,30-1,40 0,50-1,50 Kidney bean (vine) 3-6 2 Squash Melon 3-4 4-6 0,40-1,60 0,25-2,00 3 Short pepper Watermelon 3-4 5-8 0,60-3,60 0,15-1,20 Long pepper Kidney bean (low growing) Eggplant Tomato 5-7 3-6 5-7 6-8 0,40-2,80 1,00-3,00 0,50-2,60 0,35-1,50 Year average 4 5 6 7 Normal expenses. Are between 2.20 and 3.70 $/m2 distributed as follows: - E x p e n s e s directly related to crops such as water, seed, fertilizers and phytosanitary products represent 30% of total expenses. The resulting number compared to that of other cropping systems is moderately low, due to more efficient water and fertilizer use. For example, with this system (using the recommended dosage of water) its possible to obtain 44 kg of cucumber per m3 of water applied in the fall-winter cycle. In monetary terms, this represents more than $2.5 per m3 of water. - Labor expenses represent 40% of total expenses, and vary quite a bit depending upon the labor demand of the different crops. It should be pointed out here, that in one of the farms under study, labor done by f a m i l y doubles t h e productivity of paid labor. (Lopez-Galvez & Co., research under way). - Other expenses are shared between replacing the plastic on the greenhouse 76 Income $/m2 3,50-5,50 2,50-4,00 6,00-9,50 2,50-4,00 1,00-4,00 3,50-8,00 3,00-5,00 1,00-3,50 4,00-8,50 3,50-6,00 3,50-7,50 3,50-7,00 3,50-7,00 3,93-7,64 (usually every two years), changing the manure layer in the sand bed (every three to five years), and interests on circulating capital. Considering these data, internal yield rate for the 1990-1991 campaign varied between 10.4% and 18.1%. The average for the whole Spanish agrarian sector during the same period was 1.8%. 4. Micro-climate improvement in low-cost greenhouses This section examines several ideas we've been working on at the "Las Palmerillas" Experimental Station to improve microclimate conditions and increase crop productivity. As everybody knows, in order to obtain maximum productive potential, every species and variety should enjoy adequate light, temperature, humidity and carbon dioxide parameters. The object of passive greenhouses is to obtain prime products, DR. induce harvest precocity and broaden production calendars. These installations afford higher temperatures during the cold months, which are not optimum for the crop growth potential, but nevertheless result in satisfactory quality harvests which, in turn, are usually well remunerated. From the climate point of view, the main negative aspects of these greenhouses are: - High temperatures in the summer and low temperatures in the winter. - High relative humidity percentages, a n d c o n s e q u e n t l y , p r o l i f e r a t i o n of cryptogamic disease. - Lower incident radiation on the crops than on the outside. - Lower C0 2 concentration. C e r t a i n techniques facilitate the development of favorable environmental conditions inside passive climate greenhouses. Their application should consider that the different climate parameters depend upon one another. If we modify the ventilation, we'll be changing the temperature, relative humidity and air composition. Therefore, before modifying any parameter we must analyze its effect on the others. To begin with, the future greenhouse requires proper light and hence, an adequate location. Its best to choose plots that are exposed to sunlight all day, avoiding low parts of valleys, ravines and areas close to buildings or vegetation which might project shadows on the land. Also, its a good idea to keep away from valleys where cold air is difficult to evacuate, or places where fog, air pollution or other factors inhibit solar radiation. 4.1. Techniques for temperature improvement Temperature can be increased using JOSÉ LÓPEZ-GALVEZ thermo-isolating closure material. This type of material usually contains products with a low permeability to long infrared radiation (between 7 to 14 pm) which, as we know, is heat. The Spanish standard states t h a t a material is considered thermo-isolating when its transmissibility, between said wave longitudes, does not exceed 20%. There are flexible polyethylenes, EVA copolymers and PVC in the market which meet these characteristics. In Almería greenhouses its common practice to place a second, temporary, ceiling in the inside of the greenhouse to improve temperature conditions during the cold months, and the first phases of development of the crop. In this case, the material to be used should be thermoisolating, very transparent and thin, since it doesn't require great mechanical resistance. The high temperatures reached during the day, especially during the summer months, can be reduced using shades. This technique should be applied very carefully, since it's common to see shades in the inside of the greenhouse, which doesn't avoid heat penetration. That's why the shades should be placed on the outside, over the closure material, to shut out radiation. Shade screens are made of polypropylene fiber, which offers different degrees of sunlight, depending on the thickness of the fibers. This thickness determines the percentage of desired radiation. Another common technique to control high temperatures, consists in whitewashing the roof of the greenhouse or painting with any other (white or colored) material that reflects part of the radiation. Its important to use an easily washable material that can be eliminated once the hot season is over. Experience at the "Las Palmerillas" Experimental Station has shown that a larger and well placed ventilation surface 77 Improvement and control of indoor horticulture (side and top windows), used wisely can improve temperature, relative humidity and carbon dioxide concentration inside greenhouses. 4.2. Techniques for radiation improvement Any installation whose purpose is to regulate climate variables, basically a greenhouse, should try to minimize the negative impact of climate at a reasonable cost. Old constructions (using the right materials, orientation and design) resulted in acceptable environments. Modern constructions base their climates on active systems providing "environmental conditioning" by way of substantial energy consumption. In a passive climate greenhouse, the objective is to collect prime yields with regards to outdoors harvests. To that end, sowing or planting should be carried out during seasons when climate limitations don't allow outdoor crops. Passive climate greenhouses are intended to receive the greatest amount of solar energy during the months in which it is the most limiting factor for adequate development. Radiation conditions in these greenhouses can be improved during the winter months with a good selection of: 4.2.1. Closure materials A common feature of plastic materials 78 used in low-cost greenhouses is their flexibility. The most f r e q u e n t are: polyethylene (PE), polyvinyl chloride (PVC) and ethylene vinyl acetate (EVA). The degree to which these materials transmit solar radiation depends upon their properties and thickness. A good choice should take into account: - The electrical static in some materials causes suspended dust to stick, which reduces transmissibility. This is especially important in dusty areas where rain is scant. Experiments carried out at "Las Palmerillas" Experimental Station have shown that washing greenhouse roofs during the winter months increases their transmissibility between 10 and 13%. - Condensation produced on the inside of the closure m a t e r i a l can reduce transmissibility. Toneatti (1989) reports a gain of up to 15% using anti-fogging materials. To use them, however, the structures of the greenhouses have to be adapted. This type of material may have a promising future once its present limitations are overcome, since it would help reduce cryptogamic disease. The following table shows transmissibility values to photosynthetically active radiation (PAR) for two materials (antifogging EVA a n d PE) placed on a symmetrical slanting roof greenhouse (8fi angle), with an east-west axis. Measurements were taken in the southern part of the greenhouse (López Hernández & Co., 1993.) DR. 89 90 JOSE LOPEZ-GALVEZ | 91 MONTH / YEAR 4.2.2. Orientation and shape of the cover Orientation As long as there exist no other limiting factors such as the slant of the land or the direction of dominating winds, the axis of the greenhouse should be oriented eastwest or north-south, depending upon the type of greenhouse (mono or multi-hooded) and the shape of the roof. Although there is no consensus among researchers on this point, its generally standard that multihooded greenhouses are north-south oriented to avoid shadows between the hoods, except for asymmetrically roofed greenhouses, where the shadow effect can be eliminated. Crop alignment will always be north-south. Shape of the roof: when designing a roof for a greenhouse, its important to consider: - Sun rays fall on the earth at an angle determined by the latitude, time of the year and day. - Maximum radiation is obtained when sun rays hit at a 90a angle. These premises make it possible to determine the angle that better maximizes relative conditions in a greenhouse in any given place and time. So, for the Tropic of Cancer (23.5s) at solar noon, on December 21 or 22 (winter solstice), sun rays fall on the Earth at a 43 s angle. Therefore, if we wish to capture the most energy, we must give our roof a 47 s angle on its southern face, which supposes orienting the greenhouse, east-west, with the following problems to consider: - One is the size of the plot since the span of sun rays, inside the greenhouse, Improvement and control of indoor horticulture limits its width, unless we want areas with different radiation intensities. A study of an Almería greenhouse (36s 49' latitude north), with an east-west orientation, asymmetrical (8 fi slant)22m wide roof, found areas with different radiation intensity along the north-south axis, during the winter months. This clearly affected harvest precocity and, thereby its productivity (López-Gálvez, 1991). This problem can be solved by adopting an asymmetrical roof, with a larger face exposed to the sun. The surface and slant of such a face will determine the dimension of the greenhouse regarding its northsouth axis. This can be increased with a certain roof angle, by increasing the surface of the sun-exposed face, or by building several fixed hoods. In the latter case, shadows between the hoods are to be avoided. This limits the width of each hood, since the sun rays penetrating the hood are to fall on part of the surface occupied by the next hood. Besides care must be taken that part of the sun rays penetrating on one side don't exit through the other. To avoid this, we must give the side of the roof opposite the sun, a slant equal to or smaller than the incidence angle of the sun rays. (In the case of greenhouses located on the tropic of Can-cer, this angle should be smaller than 43s. 80 - Another problem pertaining to greenhouses with a slant over 20 s on their larger faces is higher ceilings and, consequentially, costlier structures, besides more operational difficulties (changing closure material, placing shades, etcetera. In sum, it is the technical-economical aspects which decide the size and slants of the asymmetrical greenhouse roof. Research carried out at the "Las Palmerillas" Experimental Station has found why these roof geometries (asymmetrical), with angles around 10fi on the sun-exposed face and 202 on the opposite face, have improved radiation conditions, t h u s increasing greenhouse productivity with respect to the traditional flat roofed, and symmetrical north-south axis greenhouses. (Lopez-G&lvez & Co., 1993.) 5. Conclusion Its possible to improve productivity in passive climate greenhouses. To that end, its necessary to: choose the right closure materials to improve thermal conditions, augmenting them with double covers; regulate temperature, relative humidity and carbon dioxide concentration by increasing ventilation surface with side and top windows; increase the intensity of radiation by guarding the orientation and shape of the roof. DR. JOSÉ LÚPEZ-GÁLVEZ 6. Bibliography BORREGO, F ; BRETÓN, J . A . ; MURILLO, M A . G., 1 9 9 2 . Forzado en invernaderos de plástico para la producción intensiva de una hortaliza mexicana de alta demanda en Estados Unidos. XII Congreso Internacional de Plásticos en Agricultura, Granada. B - 1 3 9 . M.y FLORES, LÓPEZ-GÁLVEZ, F. J.; PÉREZ, J . ; ; CASTILLA, N . BRETONES, y ELIAS, F . 1988: Comparación de medidas de evaporación en tanque evaporímetrico clase "A" dentro de invernadero. Justificación del entorno de hierba (césped). VII Jornadas Técnicas sobre Riegos. Madrid. B-12. Caja Rural de Almería. 1985. Evaluación de instalaciones de riego localizado financiadas por Caja Rural de Almería. 278 pp. N. 1986. Contribución al estudio de los cultivos enarenados en Almería: necesidades hídricas y extracción de nutrientes de cultivo de tomate de crecimiento indeterminado en abrigo de polietileno. Tesis doctoral. Universidad Politécnica de Madrid. Caja Rural de Almería. 195 pp. CASTILLA, CASTILLA, N . ; MONTERO J . I . ; BRETONES, F . ; JIMÉNEZ, M . ; G U T I É R R E Z , E . ; MARTÍNEZ, A. y FERRERES, E. 1986. Necesidades de riego en los invernaderos de Almería. II Simposio sobre "El agua en Andalucía". Universidad de Granada. Vol. I: 1992. Res- puesta al acolchado en el desarrollo y rendimiento del cultivo de tomate en invernadero, túnel y cielo abierto. XII Congreso Internacional de Plásticos en Agricultura. Granada. E-19. r a (2): 5 9 4 - 5 9 9 . LÓPEZ-GÁLVEZ, J . ; GALLEGO, A . ; BRETONES, F. 1990. Necesidades de riego de berenjena cultivada sobre suelo enarenado bajo abrigo plástico. Mérida, Badajoz. B-3. Productividad de la judía verde en enarenada bajo invernadero en Almería. Tesis doctoral. FIAPA. 225 pp. LÓPEZ-GÁLVEZ, J . , 1 9 9 1 . y BRETONES, F. 1 9 9 2 . Comportamiento climático del invernadero tipo Almería en los meses de invierno y de verano. II Congreso Internacional de Plásticos en Agricultura. Granada. B-154. LÓPEZ-GÁLVEZ, J . ; y BRETONES, F . 1 9 9 0 . Colaboración interdisciplinaria. Clave del éxito para el desarrollo de la plasticultura. XI Congreso Internacional de Plásticos en Agricultura. Nueva Delhi, India. Actas, p. 1 . 1 7 - 1 . 2 8 . JORGE, G . Con- sideraciones de tipo climático del cultivo de pepino holandés en los invernaderos de Almería. III Congreso SECH. Tenerife-España. Actas de Horticultu- LÓPEZ-GÁLVEZ, J . ; LÓPEZ HERNÁNDEZ, J . C . 91-102. IBARRA, L . y QUEZADA, M . R . LÓPEZ-GÁLVEZ, J . y BRETONES, F . 1 9 8 8 . NAREDO, J. SÁNCHEZ CARREÑO, J.; M.y CASTILLA, N . 1993."Análisis técnico-económico de estructuras alternativas al invernadero de cubierta plana parral-Almería. Investigación Agraria: Producción y Protección Vegetal. Vol. 8 (3). Improvement and control of indoor horticulture L Ó P E Z - H E R N Á N D E Z , J . C . ; LÓPEZ-GÁLVEZ, J . y Comparación de dos materiales de cubierta para invernadero: uno de polietileno termoaislante y otro un copolímero EVA con efecto antigoteo. II Congreso Ibérico de Ciencias Hortícolas. Abril, 1993. Vol. II: ARROYO, F . 1 9 9 3 . 1223-1227. A., 1 9 8 7 . Comportamiento del riego bajo enarenado en invernadero. Balance de salinidad y fertilizantes, en especial de cultivos depimientoy judía. Tesis doctoral. Caja Rural de Almería. 230 pp. MARTÍNEZ, MONTERO, J . I . ; CASTILLA, N . ; GUTIÉRREZ, y B R E T O N E S , F . 1 9 8 4 . Climate under plástic in the Almería area. E. 82 Working Party on Greenhouse Construction and Covering Materials. Karlsruhe, RFA. NAREDO, J . M . ; LÓPEZ-GÁLVEZ, J . y MOLINA, J. 1993. "La gestión de agua para regadío: el caso de Almería". El Boletín. Ministerio de Agricultura, Pesca y Alimentación. Noviembre 1993. Número 9, p p . 15-22. 1 9 9 2 . La agroplasticultura en México. XII Congreso Internacional de Plásticos en Agricultura. Granada. REYES MONTIEL, H . , "Anti-fog Films: Facts and Fiction". Plasticulture N2 8 4 . 1 9 8 9 / TONEATTI, P „ 1 9 9 2 . 4: 6-18. Corn plasticulture in France ING. L E D U J E A N Centre National D u Machinisme Agricole d u Genie Rural D e s E a u s et d e s F o r e t s ING. LE DU JEAN Contents Plasticulture Why corn plasticulture? Corn plasticulture in France Climate Soil Varieties Sowing and mulching machines Plastic films Studies of various factors relative to plasticulture in France Solar radiation energy Soil temperatures under plastic mulch Water in the soil The disadvantages of corn plasticulture Advantages of corn plasticulture Economical aspects Conclusion 85 ING. Plasticulture I ^ l a s t i c u l t u r e refers to the group of crops planted under or through a plastic mulch. There is: - A horticultural plasticulture which utilizes tunnel greenhouses and plastic mulch to produce garden vegetables and flowers. - A field plasticulture developed for certain crops such as melon, lettuce, grapes, strawberries, corn, etcetera. Corn plasticulture is practiced with clear plastic film, permeable to ultraviolet and short infrared rays (solar radiation). This clear film, however, partially seals out (approximately 50%) long infrared rays, emitted at night by the soil and the plant. Placing a clear plastic mulch between the soil and the atmosphere produces several effects; t h e m a i n one is a greenhouse effect. During the day (short infrared) sun rays warm the soil. At night, approximately 50% of the long infrared rays emitted by the soil and plant are sealed in by the mulch, thus partially avoiding a loss of heat in the soil and temperature changes harmful to the plant. This greenhouse effect allows heat to accumulate in the soil. Other effects are: LE DU JEAN - Less evaporation. Evaporation is considerably decreased by the mulch. Vapor arising from the soil condenses on the inside of the mulch, returning to the soil. When the plant emerges, it enjoys a longer period of good humidity and heat. - Better utilization of fertilizers, which no longer leach; and better phytosanitary product performance. - Soil protection. This is very important for lime soils, since phenomena such as battance are avoided, and at the same time soil structure is maintained. Erosion phenomena (wind and rain) can also be reduced when the plastic strips are adequately placed on the soil. Photodegradable low density polyethylene (LDPE) or thin (12 to 15 pm) biomechanodegradable polyvinyl chloride (PVC) are the plastics that serve as mulch in corn plasticulture. For your information, it takes two 45 kg rolls of 12 pm LDPE to cover one hectare of corn. Why corn plasticulture? Plasticulture makes it possible to grow corn in temperature limited areas without too much risk to the crop. It's used on lands which don't heat well in the spring, on battant soils, and on clay soils which 87 Corn plasticulture in France can delay the planting date. It's also used in areas which are humid in the fall or where there's a risk of frost before the crop reaches maturity. In these "limit zones" plasticulture enables producers to advance the planting date, harvest drier grains, advance maturity date, introduce slower varieties and obtain higher grain or whole plant yield for storage in silos. This illustrates how corn plasticulture is a special technique applicable to particular areas. In effect, in France, corn plasticulture is only applied in certain northern and western regions of the country, simply because to the south of these regions climate conditions (sunlight, temperature, etcetera) allow normal corn cultivation, without mulch. However, in countries north of France, lack of sunlight and risk of frost impede plasticulture. Soil types vary a lot in the limit zones where plasticulture is practiced. The climates in each of these regions also differ one from another, so it's necessary to consider varieties, planting dates and climate variations (in France 1989 through 1991 were years of drought, which was very harsh on spring crops.) Corn plasticulture is also applied in other regions to help solve very specific problems. For example, in Alsace, plasticulture makes it possible to harvest sweet corn at different intervals, so the plant industrializing the corn can double its work schedule. Corn plasticulture in France N O R D ^ ^ ^ ^ ^ P ^ ^ P A S DE CALAIS NORMANDIE \ PAYS DE LOIRE In France, corn plasticulture first appeared in 1970. In the years that followed, and until 1979, this technology was tested and studied. During this period, photodegradable plastics as well as mulching and planting machines were placed in the market. In 1983, in the colder areas of Britanny, 5,000 ha of corn 88 BRETAGNE ft f were planted under mulch. Corn plasticulture continued spreading until 1987. Then, due to good climate conditions and cases of plastic photodegradability, B r i t a n n y showed a drop in acreage planted under mulch. The subsequent drought years, however, triggered the reinstatement of this technique in the province. ING. NUMERO HECTAREAS 30000-r LE DU JEAN C O R N PLASTICULTURE USE IN BRITANNY FROM 1981 TO 1991 25000 - - 20000-- 15000 - - 10000-- 5000-- 1981 1982 1983 1984 1985 1986 1987 1988 1989 1990 1991 YEAR At the same time, in the rest of France, mulched surfaces continued to grow also. Total in France for 1986 is estimated at 32,000 hectares, and for the 1991, the number is 70,000 hectares. Plasticulture is practiced in Aquitaine, Midi-Pyrénées and Alsace, but it is mainly in the regions from La Loire to Britanny, Normandy and northern Picardy where the greatest extensions are dedicated to corn plasticulture. Surface of corn grown under mulch in Britanny in 1991 Mulch cultivated corn in Britanny in 1991 Armor Coasts 16,000 Finistere 6,000 Morbihan 4,000 lile et Vilaine 3,500 89 Corn plasticulture in France Mulch cultived corn he•ctares (AGPM estimate 1990 1986 1989 1,300 3,000 Northern Picardy 6,400 1,500 5,500 2,000 Total in France 32,000 La Loire Normandy 1991 5,500 8,700 9,500 14,500 4,500 3,000 65,000 to 70,000 45,000 Source: Carpentier, 1992. Corn plasticulture is especially applied in the coasts of Armor, representing 11% of total land dedicated to corn in that department. Total for France in 1992 is estimated at 70,000 hectares, but in 1993 we witnessed a 30% drop in mulched surfaces, so total surface for 1993 should be the same as that achieved in 1990, in other words, 40,000 hectares. This decrease didn't arise from climate conditions nor any other t e c h n i c a l factors, but because of uncertainty brought about by PAC and GATT, talks which prompted growers to postpone investments and reduce costs. RGURE 16. SUM OF SPRING 90 MEAN (1951 TEMPERATURES O V E R - 1975) 4=C Climate Corn plasticulture allows improved conditions when the corn cultivation process begins, so a satisfactory level of yield and quality can be anticipated. Plasticulture is applied in regions where cool spring temperatures limit corn production. The following two maps show limit zones for corn cultivation, where the sum of temperatures is between 1400SC and 1500 2 C/year (western and n o r t h e r n France). MAP 40. "GRAIN" CORN SPRING PLANTING (1951 - DATE 1975) ING. LE DU Tests carried out by AGPM*, ITC**, INFRA*** and the National Meteorological Institute show that the best external measure related to corn evolution the grower himself can obtain, is the sum of temperatures over a 6SC limit, approximately (below this temperature, the plant is in a stage of retarded growth). This criteria makes it possible to obtain the best forecasts of blooming and harvest dates. JEAN Between sowing and female blooming, temperature needs are closely related to the precocity of the hybrid. The later the variety, the greater its need of heat to reach blooming. Differences between hybrids essentially arise from the fact that they don't produce the same amount of leaves: earlier varieties only produce 13, while later ones produce up to 19; hence, differing blooming dates (Table 1). Table 1 Sum of temperatuires (degrees) necessary for hybrids of different precocity to achieve ilooming; number of leaves observed at blooming Sum of Number of Varieties mean temperatures leaves at blooming Bovee LGI 7702 13 to 14 leaves s Ema 770 15 to 16 leaves s Brulouis Inra 180 785 15 to 16 leaves s Liza 825 15 to 16 leaves Oca 850s 16 to 17 leaves s Brio Grow 42 930 18 to 19 leaves s Eva 960 17 to 18 leaves s Autan LG 22 980 18 to 19 leaves s Concorde Pau 560 1,030 19 to 20 leaves s Ferax 1,075 19 to 20 leaves Sources: AGPM-ITCF. Studies on the evolution of water content in the grain, show that for the blooming-maturity period, water content is closely related to the sum of temper a t u r e s received by the plant since * AGPM: General Corn Producers' Association. ** ITCF: Technical Institute of Cereal and Fodder. * * *INRA: National Institute of Agronomic Research. feminine blooming. When these aren't limit temperatures, maximum grain weight is obtained with a 32% water content (Table 2). Corn plasticulture in France Table 2 Sum of temperatures (degrees) necessary to pass from blooming to stages 38, 35 and 32% of grain water content for hybrids of different precocities Sums of mean temperatures at stages Test years Varieties 35% 32% 38% Bovee LGI Ema (1) Brulouis Inra 180 Liza Dea Brio Asgrow 42 Eva Autan LG 22 (1) Concorde Pau 560 Ferax (1) 1979 to 1982 1984 to 1986 1982 to 1985 7352 795 s 7552 7402 820 s 8002 1980 to 1981 to 1977 to 1983 to 1984 to 7602 7902 775fi 8052 825 e 8352 820 s 825 s 850 s 835 s 860 s 880 s 890 s 875 s 1985 1986 1983 1986 1986 1980 to 1984 1984 to 1986 870 s 895 s 930 s 890 s 925 s 935 e 960 e 940e Sources: AGPM-ITCF. (1) Provisional results. With these data it was possible to establish a corn cartography, scaled to France, taking into account climatological risks. The sum of temperatures separates the date of the first frost of autumn (see the following map). The g r e e n h o u s e plasticulture heats the months. This increase sum between 1202 and MAY 3 MAXIMUM TEMPERATURE 18 9 C MINIMUM TEMPERATURE 10 s C M E A N TEMPERATURE 18_t_lQ 1 4 2 C 2 EFFECTIVE TEMPERATURE Ü 14 - 6 E 3 MAXIMUM TEMPERATURE •34 g C MINIMUM TEMPERATURE • 16 2 C C JULY 30 UMIT MEAN TEMPERATURE 30_M6 2 3 2 c EFFECTIVE TEMPERATURE 23 - 6 E 3 3 c 92 865 e 895 s effect due to soil for about two corresponds to a 150s on base 6. ING. LE DU JEAN Calculation of effective temperatures for corn Each day, effective temperature is the average temperature under cover, over the 62C level. Effective temperature = Maximum temperature + minimum temperature _ g„ 2 For a maximum of 18SC and a minimum of 102C: - average temp, is 14s C - available temp is 82C Whenever necessary, two correction factors have been considered: when mean temperature is under 6 e C, effective temperature equals 0. When maximum temperature is over 302C, this value is limited to account for stress. For a given period, the sum of effective temperatures for corn corresponds to the sum of daily average temperatures over 62C. Soil Soils are very different even in the same limit temperature areas. They range from the granite soils of Britanny to the calcareous e a r t h of N o r m a n d y and northern Picardy, with all the variables these two soil types entail. (In the following map, granite soils with an acid pH can be found in the west, while calcareous soils with a neutral or basic pH are located in the central and eastern regions of the northern part of France.) 93 Corn plasticulture in France Varieties Varieties used in northern and western France (in 1993): - Northern Picardy-Normandy: DEA, DK250, Fanion, Nobilis - Ille et Vilaine-Armor coasts: DEA. DK250, Nobilis, LG2250 - La Loire: Barbara, Corsaires, DEA, DK250, DK300, Fanion, LG2310, Nobilis. Some new varieties have given good results, for example: - Armor coasts: Anjou 207, DK 232 - La Loire: Anjou 305, Bilboa, etcetera. The potential of any variety is related to its precocity with later rates. Limits are reached quickly (maturity, increased drying costs.) In agroindustry, grain hardness; in PRECOOIY other words the type (corne and corne dente) are fundamental to the starch and flour industries. Criteria determining varieties destined for animal feed (whole plant for storage in silos or moist grain) are dry weight production per hectare, flavor quality and DMO (digestability of the organic matter of the fodder). Depending upon the variety selected, planting density must also be determined so the corn can fully develop its potential. For example on the Armor coast, the most used varieties are DEA and LG2250, with a precocity rate between 250 and 300. For mulch-grown corn, slower and therefore more productive (90,000, 105 plant^/Ha), hybrids are used (+50 precocity index). DENSITY BY PRECOCITY POPULATION / HA 70,000 80.000 90,000 100,000 110«» 120,000 VERY PRECOCIOUS PRECOCIOUS SEMI-PRECOCIOUS SEMI-PRECOCIOUS SEMI-LATE LATE VERY LATE BEDS, PRODUCT QUAUTV « D ECONOMICAL OPI1MUM SHOULD BE CONSIDERED BEFORE SELECTING THE DENSI7V FOR A DETERMINED PRECOCilY RATE. SOURCE: A3PM - I1CF 94 ING. LE DU : 0.15 m 0,75 m. 0,15 m WIDTH OF THE FILM: 1.35 m. A 0.25 m ^ 0.25 m ^ — • 0.25 m • 0.25 rn * W * ^m m WIDTH OF THE FILM: 0.64 m. SOURCE: JEAN71L Certain equipment can be added on to the machines: - microgranulators (insecticides) - systems to avoid grain waste, etcetera. T h e s e m a c h i n e s are, t h e r e f o r e , very specific, and since their appearance in t h e m a r k e t in 1976, technical innovations have been directed towards reducing their weight. This enabled the use of less powerful, lighter tractors, which in t u r n reduced soil compression. Sowing under mulch is a delicate operation. It requires an experienced driver and reduced speed: 1.5 b/ha. We e s t i m a t e t h a t a sowing and mulching machine can plant between 100 and 150 hectares per year. Quality sowing requires t h a t the plastic film be well buried in the ground so it doesn't fly off. Also, perforations much be sufficiently spaced (15 to 25 cm) to limit the action of the wind. This JEAN Sowing and mulching machines These machines carry out several operations: - prepare beds - place and fix mulch on soil covering edges and row ends with soil. - perforate the mulch with a spine wheel. - place one or several grains in the holes made by the spines (possible depth adjustment). Machines are capable of sowing two or four rows in single step and are adjusted to the widths of the plastic strips being used. (Plastic film strips measuring 1.35 m or 0.64 m.) Sowing is carried out as follows (see sketch). involves multiple sowing, i.e. one or several seeds per hole, in order to obtain desired density. Certain users prefer narrow strips (0.64 cm) to limit the action of the wind. Plastics films Films used in corn plasticulture require good mechanical resistance to avoid any tears during installation. This also implies very good soil preparation before mulching it (eliminating stones and clods). Film should remain in its place six to seven weeks (greenhouse effect) after which it should autodestruct so it doesn't get in the way of subsequent crops. At present, in France we use two kinds of films. - P h o t o d e g r a d a b l e low d e n s i t y polyethylene (LDPE) films. - Biomechanodegradable polyvinyl chloride (PVC) films. 95 Corn plasticulture in France Plastic LDPE-based films Low density polyethylene comes from a petroleum by-product called NAFTA. The resin base of the film is made of this polymer to which an absorbing agent (a coordination compound made of iron, cobalt, nickel or manganese) is added, making it photodegradable. The first films used, made of LDPE resins were 30 pm thick and practically weren't photodegradable. Later, linear LDPE film (with a different linear structure) was introduced, and its higher mechanical resistance allowed thinner films. Since 1981, 15 pm films have been on the market, and in 1984 12 pm films were introduced. In France today there are at least four different processes to manufacture these plastic films. In c e r t a i n processes industrials introduce a compound or mix of polyethylene resins (linear, radical) and photodegradable agents. By extrusion, this compound is transformed into agricultural mulch film. Certain manufacturers don't use a compound, and carry out the extrusion process by mixing polyethylene and an additive in continuum. According to our data, there are seven extruders in France whose LDPE film is produced for agricultural use. LDPE based film rolls, sold for corn plasticulture, are 1.35 m wide, 3,100 m long and are used for mulching two rows of corn. A roll weighs about 45 kg, and represents a film surface of 4,185 m2. Approximately 930 m2 are buried (15 cm on each side) so one hectare of corn requires two rolls of mulch. A description of the photodegradable process follows (Fanton, Gazel and Lemaire, 1984): The coordination compound absorbs photons coming from the ultraviolet 96 radiation of the sun, originating free radicals. These react to the polyethylene chains creating macro-radicals, which oxidate forming groups, which in turn absorb sun photons. This succession of photochemical reactions causes a rupture in the polymer chain. In sum, it is a fairly rapid photofragmentation of polyethylene chains, depending upon the luminous intensity absorbed and the concentration of the absorbent additive in the field. Degradation starts with the appearance of tears on the film: the plastic crystallizes and fractures. We were unable to gather precise information on the minimum size of polyethylene chains after the degradation process. It's likely that these inert chains aren't bio-assimilative due to their length, (more than 35 carbons). Buried film edges can't receive UV radiation and are not photodegradated during the cultivation year, and won't be unless they reach the surface in the course of subsequent years. Muddy film can inhibit the degradation process described above, so it's easy to find remains of these buried edges a long time after their first use. PVC-based plastic film Base polymer for this mulch is polyvinyl chloride, which comes from an oil ethylene and chloride from marine salt. (Chloride makes up 57% of the polymer.) In its un-refined state, this polymer is hard and must be plastified. This is accomplished with vegetable or synthetic plastifiers. PVC mulch films also contain stabilizers (zinc, calcium, and sometimes heavy metals such as nickel, cadmium and lead). In France there's only one producer for this kind of plastic, he's been manufac- ING. LE DU turing film for agricultural mulching since 1985. His plastic films are 15 pm thick and come in narrow 0.64 m strips, between which a row of corn is planted.The distance between two consecutive rows is 0.75 m. A four row planting and mulching machine holds four rolls of film. The PVC plastic film manufacturer states that all the components in his product are registered for contact with food. Theoretically, then, the film doesn't contain heavy metals. The degradation process followed by this film is complex. On one hand it's a biodégradation of the plastifiers, and on the other, its a photo-oxidation: additives disappear, the PVC chain fragments. In this case we don't know either how small the polymer chains can get, but they're probably not bio-assimilated. The degradation process of the film is characterized by progressive peeling even after the harvest. The film carries on worse until it looses elasticity and becomes fragile. Buried parts also degradate, so the soil is completely f r e e d of plastic, which isn't the case with photo-degradable film. Studies of various factors relative to corn plasticulture The following results come from experiments performed at the CEMAGREF of Montoldre, in the Department of L'Allier, JEAN France, where corn cropping in uncovered soil and under plastic mulch are practiced on light, washed, drained soils with a low water reserve. The results concern: - solar radiation energy, - soil temperatures under plastic mulch, - the evolution of soil water content. Solar radiation energy Photodegradation of a polyethylene sheet is linked to the amount of ultraviolet radiation provided by the sunlight absorbed by the film. Measurements were taken with the aid of two pyranometers. One was placed on one of the plastic strips in the corn field 0.3 m from the surface. Solar radiation energy is given in kilojoules per square meter. With this device it was possible to know the amount of solar radiation received by the plastic. This value was then related to the value captured in a nearby meteorological station also equipped with a pyranometer. Results show that during the first forty days of the vegetative cycle of the corn, the plastic film received the same solar energy as the station. After the first 55 days of the cycle, however, energy for the plastic sheet isn't more than 50%. This decrease is related to the vegetative development of corn and the height of the plants over the mulch. (Between June 10 and 30, 1986, mulched corn grew 6.4cm/day.) 97 Corn plasticulture in France PERCENTAGE OF SOLAR ENERGY RECEIVED BY THE MULCH WITH RESPECT TO THE SOLAR RADIATON ENERGY EMITTED EVERY DAY % 110 100- 9080- \ 70- fiO- Î t I 3 m. HEIGHT OF CORN PLANTS 60- t., \ 130 cm.64 DATE 5/5/86 165/86 19/5 20/5 21/5 22/5 23/5 26/5 27/5 10 15 104,7 % 94,8 % 101,5 % 92,8 % 106,1 % 99,7 % 102,4 % 101,0 % 20 25 28/5 29/5 30/5 2/6 3/6 4/6 5/6 6/6 30 5/6 100,6 % 99,7 % 102,2 % 102,8 % 103,6 % 102,3 % 104,8 % 101,2 % 10 15 20 9/6 10/6 11/6 12/6 13/6 16/6 17/6 186 25 30 100,6 % 100,2 % 98,8 % 96,8 % 105,7 % 105,7 % 97,4 % 95,3 % 19/6 20/6 23/6 24/6 25/6 26/6 27/6 30/6 13 to 14 15 to 16 97,3 % 97,3 % 77,2 % 66,8 % 65,7 % 53,1 % 59,7 % 49,7 % Example from 9/6/86 T. U. in hours 4 to 5 5 to 6 E n e r g y r e v e i v e d by t h e s h e e t in kilojoules/m2. E n e r g y received b y t h e meteorological s t a t i o n in kilojoules/m2. Energy %: plastic sheet/meteorological station 6 to 7 7 to 8 8 to 9 9 to 10 < 98 11 to 12 12 to 13 14 to 15 16 to 17 17 to 18 18 to 19 860 300 1,584 2,400 2,829 3,151 2,903 3,192 3,037 120 510 1,270 1,840 2,450 2,840 3,050 2,780 3,060 2,950 2,600 2,110 1,520 86,0 97,9 99,6 Total energy received by the station between 0H00 and 24H00 from 7H00 to 14H00 Total energy received by plastic sheet from 7H00 to 14HOO Energy %: plastic sheet/meteorological station from 7HOO to 14H00 * 10 to 11 103,3 104,4 104,3 102,9 28,410 kilojouleVm2. 18,970 kilojoule^m2. 19,096 kilojoule^m2. 100,6 % ING. LE DU JEAN Soil temperatures u n d e r plastic mulch (greenhouse effect) vered soil. This shows the significance of the greenhouse effect caused by mulch even in deeper layers of soil during M e a s u r e m e n t s were t a k e n with the first 55 days of the crop; and also thermocouples placed identically on the shows a fall in the thermal effect of mulch soil at different levels under the plastic as soon as the plant displays foliage, mulch and on naked soil (traditional crop). limiting the amount of solar radiation Results indicate temperature differences reaching the ground. (In this case, on at the same levels in mulched and unco- 15/06/86.) C SOIL A V E R A G E T E M P E R A T U R E D I F F E R E N C E BETWEEN M U L C H E D A N D U N C O V E R E D B E T W E E N 9 h 0 0 A N D 1 5 h 0 0 D U R I N G P E R I O D S O F 5 D A Y S A T A DIFFERENT LEVELS ( G R E E N H O U S E EFFECT) 10 » 8> LEVEL O c m (SOIL SURFACE) 6 S LEVEL - 1 0 c m »• 3 LEVEL - 2 0 c m 2 »• • LEVEL - 6 0 c m DATE 5/5/86 10 20 25 25 EVOLUTION O F WATER IN THE SOIL % O F WATER IN THE SCHL JUNE 78.000 m m PLUVIOMETRY - JULY 3 0 , 0 m m A G O S T 26,4 m m 24/5/85 | 30 31/6 y6/85 25/630/6 1/7 8/7 ___ —I—i—i—I 16 2023/7 26 n 1 31/7 6 1/8 1— 8 P 30 31/8 2/09 99 Corn plasticulture in France The results shown here are from the 1985 experiment. Water content in the soil was measured through soil samples taken between 0 and 30 cm, 30 and 60 cm, 60 and 90 cm with the help of a drill. Samples were taken from a mulched plot and a traditional (uncovered) witness. Water content was measured by two series of four tensiometers placed close to the sampling area at 30, 60, 90 and 120 cm. Level 0-30 cm. Water reserves in the soil during June were enough to feed corn under plastic mulch. It seems that during the first month, the amount of water retained by the soil was more significant than that in the witness soil. The plastic film formed a barrier limiting evaporation. However radicular exploration of corn under the mulch was more important than for the witness. Indeed, the strong development of mulched corn caused a greater need for water. After June 25, the difference in mulched soil water content as opposed to that in uncovered soil increased. After July 18 the 0-30 cm layer of mulched soil had no water left for the plants. This date is marked by a fall on the tensiometer graph. On the witness plot, tensiometer fall was July 28, that is, ten days after. Level 30-60 cm. Water content in mulched soil was lower than in the witness. The significant growth experienced by mulched corn created a greater water demand. Soil water was absorbed by a more deeply developed and deep radicular system; hence lower water content at that level. By the first days of August, the combined effects of this greater water demand and the drought, resulted in water depletion at that level for mulched soil. This development is attested to by the tensiometer drop at that level. This lack of water for the plants since the first week of 100 August at the 0-60 cm level proves the weak water retention capacity of these lixiviated to "psuedogley" drained soils. The evolution of the relative curve at the 30-60 cm level in the witness corn crop indicated that by the first days of August, water content was the same as in the mulched crop. This only proves the poor water retention of these soils. 60-90 cm level. The effect of the drought can be appreciated by the beginning of August, but the mulched soil was more humid than the witness at this depth. Several reasons can explain this higher water content: the strong development of the corn cast more shade on the ground. The consequentially un-degraded film served, throughout the experiment, as a barrier which limited water evaporation. Two series of three tensiometers were used in the 1986 test. These were placed on mulched corn: one on the irrigated part of the plot, another on the non-irrigated part of the plot (to study the 60, 40, 20 cm levels under the mulch.) It rained between May 12-23, right after planting. 88.6 mm of rainfall completely replenished the water reserves in the soil. A dry period followed in June (12.4 mm pluviometry), and in July (14.4 mm pluviometry.) The curves made by the values set by the tensiometers show the evolution of water in mulched soil: The 0-20 cm layer dried quickly between June 15 and 25 on non-irrigated mulched corn. The 0-40 cm layer dried just as fast between June 20 and July 5. The 0-60 cm layer dried slower between July 10 and August 10. Falls in the tensiometers at the three levels occurred on June 25, July 11 and August 12, respectively. On irrigated mulched soil, water evolution in the soil proved comparable to ING. LE DU % WATER IN THE SOIL JEAN SOIL (0-60 c m ) WATER EVOLUTION UNDER PLASTIC MULCH 0-60 c m 0 TENSIOMETER (CENTIBARS) 13 NOT IRRIGATED IRRIGATED l n *, 20 D = Tensolmeter fall - 6 0 cm 1/6/86 1 4.6 7,8 IRRIGATIONS ACUMULATIVO DE TEMPERATURAS 1/9/86 1/7/86 0.0 4,2 14,4 J_ 1 J 2,5 L 17.0 1 27,2 H—PLUVIOMETRY (mm) 9,0 I ACUMULATIVO DE TEMPERATURAS BASE 6 2 C Y FECHA DE FLORACION (50% PANICULAS FEMENINAS) 1500 J 1000 I LG 2 2 5 0 S E G U N D A SIEMBRA PLASH F I C A D A . IRRIGADA 500 H LG 2 2 5 0 PRIMERA SIEMBRA PLASHRCADA IRRIGADA LG 2 2 3 0 IRRIGADO FECHA MAVO 1986 JUNIO JUÜO 17/7 30/7 AGOSTO SEPTIEMBRE 9/8 101 Corn plasticulture in France that of unmulched and non-irrigated soil, except that it was slower. The curve for the -40 cm level shows the influence of irrigations, but it was inevitable the soil should dry. Tensiometers fell on August 1. On a year with a dry summer this kind, of permeable soil has insufficient water retention to optimize corn plasticulture, therefore, irrigation is a must. These results on water evolution in mulched soil show how important it is to know the water reserve (RU) or the readily usable water reserve (RFU), in the soil when corn plasticulture is contemplated. In France we can observe that north of La Loire a n d n o r t h e r n Picardy, corn plasticulture is only practiced on deep plots which are cold in the spring, and have enough RU or RFU (between 100 and 120 mm of water.) When a crop manufactures a kilogram of dry weight, it means that approximately 600 liters of water are extracted from the soil. The twelve tons of dry weight produced in a hectare of corn, for example, require 7,200 m 3 of water per hectare; that its, approximately 720 mm. These 600 liters per kilo of dry weight must be increased 50% south of the 45th parallel (Bordeaux, France) and 100% south of the 40th parallel (Madrid, Spain.) Soil RU and RFU are measured and calculated (See Soltner document: The Soil) by measuring the apparent soil density, Da; the equivalent humidity, He; and estimating the depth of the soil exploited by the roots, P. The formula is RFU in mm = 3.Da.He.P. The disadvantages of corn plasticulture There aren't very many, and they are mainly related to ecology; particularly 102 accumulated plastic waste in the soil when photodegradable plastic is used. Indeed, it's not unusual to find in a crop almost intact strips of plastic, after a rake has passed by, fracturing already fragile plastic into small pieces. Polyethylene fragments return to the soil during preparation and end up saturating the layer being tilled. This, without considering the buried strips that once held down mulch, which haven't degradated. The aforementioned, however, isn't the greatest disadvantage of corn plasticulture. Indeed, the plastic cover over half of the cultivated surface can remain intact during the vegetative cycle of the corn and become a handicap to the plant. In dry summer years and on light soil, completely dry soil 0-40 cm under the mulch doesn't consititute a good element for optimum corn development, since it limits air-soil exchanges. It is therefore imperative t h a t photodegradable low density polyethylene mulch function as intended, effectively degradating two months after its placement. At present, this LDPE mulch degradating process seems to be well mastered by manufacturers. But the problem arising from buried parts still remains to be solved. Advantages of corn plasticulture The a d v a n t a g e s are m a n y when pedoclimatic conditions are adequate. The plant benefits from the greenhouse effect, that is: - soil temperature increase, - limited soil evaporation. The soil maintains its structure (zero battance on lime soils) Plastic mulch increases heat which accelerates corn development, making for quicker germination (precocity after 5 days, approximately), faster vegetative ING. development (corn vegetative cycle advances approximately 3 weeks), deeper and faster radicular growth, which benefits water utilization. This precocity afforded by mulch makes it possible for feminine panicles to bloom before soil water reserves have been exhausted. In most cases, this precocity generates higher yields whether in grain or whole plant dry weight. In Britanny the most complete study LE DU JEAN done on increased yield and dry weight for silo was carried out by the Reseau National D'Experimentation et de Demonstration (RNED) Cereales. Thirty tests were performed from 1983 to 1985 (12 reference plots, two of which were in the Armor coasts) to measure the effect of mulch on silo corn production, with the same varieties used on naked soils and with later varieties. Compared silo corn production of mulched and naked soil Later varieties Same varieties 55 31 Number of comparisons + 4.4 t MS/ha + 3.4 t MS/ha Increase in mean yield + 4.9 % + 3.8 % Average increase in dry weight content Source: R N E D C e r e a l e s B r e t a g n e , 1986. Increases in yield vary greatly from one test to another. In varieties with different precocities, this can be nil or over 9 t m$/ ha. These different results are brought about by a combination of different f a c t o r s : region a n d selected plots, C O M P A R E D YIELD BETWEEN M U L C H E D / N A K E D ( U S I N G LATER VARIETIES F O R PLASTICULTURE) L-UIYIKAI^IbUINS + 0 + 1 experience with the technique. Other studies, such as the one made by the EDE on the northern coasts in 1981, 1982 and 1983 (Blondel, 1984) highlight the great differences in yield increases obtained thanks to mulch. +2 +3 +4 INCREASE +5 +6 I N YIELD ( I N +7 +8 SOIL +9 +10 MS/HA) 103 Corn plasticulture in France There is also an increase in yield for the grain harvest. These experiments aren't as in-depth as those performed on whole plants for silo. Using the 1986 Montoldre experiment as an example, we had an average increase of 6.6 points of dry weight and 1,880 kg of grain dry weight per hectare of mulched corn with respect to the witness. Expressed in commercial grain amounts, mulch produced an increase of 22 quintal^ ha with respect to the witness in this experiment. We found a more important gain in production, an earlier harvest date, and a greater amount of dry weight for the grain corn. However, generally speaking, it seems that plasticulture is less important for grain corn than for silo corn, and that the ratio of ears on a whole plant doesn't always improve; but this remains to be proven. These gains in yield require an increase in fertilizers. animal raisers contemplate plasticulture as insurance against lack of silo reserves and a guarantee of good quality silo corn. This insurance, however, is difficult to calculate. Nevertheless, plasticulture can't be systematically recommended, given the wide diversity of results. An interesting study carried out in Britanny by RNED Cereals compared, in the course of three years, more than thirty cultivation tests on naked and mulched soil, including two varieties with different precocity and two sowing techniques. DIFFERENCES IN YIELD DUE TO MULCHING Economical aspects In contrast to traditional cropping, this t e c h n i q u e implies a more or less substantial extra cost depending upon the exploitation. Amortization of these costs requires a yield gain between 16 and 23 dry quintals, in the case of grain corn, and between 3.3 to 4.7 tons of dry weight in the case of silo corn. Investment amounts to 2500-2700 francs per hectare including plastic film, machine sowing and additional fertilizers necessary to achieve expected increase in yield and financial costs. Traditional crop costs are deducted from this total. Following this line of thought, the yield increase should free hectares so they can be dedicated to other crops. Margin from the latter crops should be higher than the cost of plasticulture to free said land. Many 104 This figure shows yield differences in one variety due to mulch. It can be observed that yield is improved with mulch, but also that in 62% of the cases, yield is under the 3.5 tons of dry weight which constitute the economical minimum. ING. LE DU It can be seen that in spite of the benefits of this technique, when the grower decides to apply it, he must be aware of the financial risk involved. It should be noted that at present, the grower wishing to apply corn plasticulture can find support with a better pedoclimatic knowledge, particularly with respect to clay, lime, humus and RFU percentages, as well as v a r i e t y selection a n d t e m p e r a t u r e calculations. If experimentation were carried out today, the cloud of doubts would be narrower. Conclusion Corn plasticulture is developing in France. Until now, however, it is mainly concentrated in the northern and western part of the country, and more attractive to silo corn growers than to grain producers. Techniques consist in mulching with wide photodegradable LDPE plastic film or narrow biomechanodegradable PVC s t r i p s . Available sowing-mulching machines are adapted to both techniques and work smoothly. Their operation, however, must be slow and require an JEAN experienced driver. Greater progress is possible. Present research is geared toward reducing the amount of plastic used and also to decrease weeding. At present, plots are weeded prior to planting. A second weeding is carried out during early development of t h e p l a n t s w i t h a p r o d u c t called "Dinoterbe", which can penetrate the plastic sheet. It's very likely, though, that this product will be banned in the near future. Regarding the environmental point of view, photodegradable plastic leaves problematic residue in the soil. A new corn plasticulture method is under trial. It consists in using non-photodegradable polyethylene which could be removed during the seven-leaf stage of the plant with a machine capable of pulling it out of the earth, cleaning it, and rolling it (DBE) at a rate of 6.5 km/h without damaging the plants. Trials are being carried out with 17 pm plastic films, with the hope of eventually using a 12 pm film. Plasticulture, therefore, is not a static technique and allows new developments. 105 Corn plasticulture in France Bibliography Culture du maïs en Champagne crayeuse avec films plastiques photodégradables. Cahier des ingénieurs agronomes, n 2 306, Mai 1976. DUTIL, P . La photodégradabilité contrôlée des films de polyéthylènes agricoles en Israël. Plasticulture, n2 30, Juin 1976. sol. Production laitière moderne, n 2 145, Février 1986. DESPLANTES, LE Du, WIERZBICKI. Plasticulture du maïs. BTMEA, n s 7-8, Juillet-Août 1986. GILEAD, D . Les bases de la production végétales. Tome 1: le sol. SOLTNER, D . Les applications agricoles et horticoles des films de polyéthyléne (PEbd) photodégradables. Plasticulture, n 2 41, Mars 1979. Atlas agroclimatique saisonnier de la France, 1980. HANRAS, J . C H . GAZEL ET LEMAIRE. Fiabilité du film photodégradable agricole. Caoutchouc et plastiques, n2 641, Mai 1984. LE DU, J. Photodégradation des films plastiques en semis. BTMEA, n2 22, Octobre 1987. La plasticulture du maïs en Bretagne. Document du Conseil Général des Côtes d'Armor-Mars 1992. M. Les équipements de plasticulture. Motorisation, Novembre 1993. Nouveautés - Désherbage - Résultat des variétés. Entraid, Janvier 1994. POTTIER, FANTON, Deux facteurs limitants pour le maïs: la température et la structure du PERROT, M . 106 Mais: adapter la variété à la parcelle. Top Culture, n 2 33, Janvier 1994. Carte des sols et des sommes de température. Document Maïs Adour. VERSANTI, L . Web vegetable cultivation as compétitives tools ING. ALEJANDRO GÁLVEZ Invernamex INC. ALEJANDRO . ^ ^ n extremely ample and varied range of possibilities is available through the use of plant tissue cultivation techniques (i.e. micropropagation), not only because of the complexities of the process, but because of their applications. It is here where several aspects come together. First the application should be evaluated so the producer pays its total cost; second, it is to be carried out in a way that is comprehensible to the producer and practical within the production system he may have. Finally, the value of the material plus its implementation in the production system must be affordable, and provide benefits making it more productive. Producers face a series of problems which t h e y m u s t solve d u r i n g t h e production process. I'm referring to biotic, edaphic and climate related problems. Some of these can be dealt with beforehand, and others, as they arise. Nevertheless, the economical success of the crop will depend upon a satisfactory sale of the production, which is why we never know how much we'll be making until the last day of the sale. It is clear that problems during the production process, cause reductions in yield and, very likely, in quality. This makes us liable to countless risks, which are very hard to measure and that we GÁLVEZ learn to prevent with every cycle. Each exploitation must be considered as a profitable, well managed, business. In the farming business, producers have to take into account several sensitive issues, which u s u a l l y pose control problems. These are costs, losses and risk prevention. We'll briefly examine these three concepts to identify their consequences and the essence of quality. The quality of agricultural species, from my point of view, is poorly understood and analyzed in essence, since we seek better quality in order to sell the final product. However, final quality is a response to every moment of the production process, and depends upon the quality of the labor, production techniques and response to unforeseen difficulties. Final quality not only implies a better sale and price, but also t h a t during production there were fewer losses, more efficiency, technical capacity and a better use of resources. This, logically results in lower production costs and higher profits. A lot has been said about the benefits t i s s u e cultivation can give to any production, nevertheless it has been scarcely understood. In almost any publication we can read about t h e advantages of these techniques and how 109 Web vegetable cultivation as competitiones tools they interrelate with costs, losses and risk a p p r a i s a l . The following are some examples: - Genetical homogeneity. - Disease-free plants. - Potentially productive plants. - Better response to collateral technologies. - Selection of outstanding plants. - Quick multiplication. - Simplification of genetical improvement processes. - Higher quality. - Recovery of original characteristics. - Higher production. - Lower production costs. The relationship between these technologies and competitiveness for the producer and the nation is evident. Regarding Mexico, the following aspects are worth mentioning: Ecology. Tissue cultivation companies can produce millions of plants in a relatively small space. Even the smallest lab has to produce between two and three million disease-free plants. Technological development. These lab- si 110 companies absorb information on and specialize in problems proper to each crop. Afterwards, they furnish very precise information on production systems that are both safe and profitable. Plant health. The production systems and goals of these labs adhere to legislation set forth by the Mexican Agricultural and Water Secretariat, with who they share equal goals in favor of the public. Germ Plasm. Little has been achieved in this country along these lines, and there is still a strong dependence on international sources. Plant tissue laboratories should therefore have their own germ p l a s m b a n k , which is e s s e n t i a l to commercialization purposes and for resource optimization. In certain cases they will have to implement genetical improvement in t h e search of new varieties. The consolidation of a strong relationship between production processes and plant tissue cultivation techniques will result in more productive businessmen, more profitable businesses and competitiveness for all Mexicans. Automatization of irrigation and nutrition equipments ING. A V I IVANIR Training and Service in Latin America ING. AVI 1. Physical structure (cables, solenoids, water meters, fertilizer meters, valves, etc. 2. Maintenance (operation and maintenance). 3. Choice of the adequate system. The advantages of irrigation automatization 1. Savings in water consumption. 2. Savings in energy consumption. 3. Savings in transportation. IVANIR 4. Savings in labor. 5. Increases f e r t i g a t i o n efficiency (through better pressure, wind and humidity conditions, among others). 6. Increases fertilization efficiency. 7. Improves retro-washing efficiency. 8. Sampling in true sensor time. 9. Irrigation emergency programs, in the case of frost and heat waves. 10. Better pump operation. 11. Data concentration. 12. Network safeguarding. 113 Diseases and soil solutions D R . ALBERT O . P A U L U S P l a n t P a t h o l o g y , U n i v e r s i t y of C a l i f o r n i a DR. S o i l fumigation is practiced on various vegetables and strawberry crops in the United States of America. During the 194050's growers usually planted two to four successive crops and then moved on to new virgin ground. But the virgin land gradually became unavailable to growers because of housing and commercial developments and the prohibited cost of clearing what virgin land was availabe. New irrigation systems were costly to install on the new lands. This lead to a condition known popularly as "old land disease", which was thought to be due to a complex of fungi, nematodes, weeds and deterioration of soil physico-chemical properties. This lead to decreased tomato, strawberry, etc. yields with successive crops. Full scale fumigation of tomato fields in Florida was too costly yet in-the-row fumigation for short-term nematode control proved inadequate for long season t r e l l i s - g r o w n t o m a t o e s in Florida. Research showed that polyethylene films used as mulch could enhance the performance of fumigants on large acreage (Bewick, 1989). Soil Fumigants Methyl bromide (bromomethane) is one of the most commonly used fumigants for ALBERT O. PA ULUS the control of fungi, nematodes, soil insects, weeds and has some effect on bacteria and virus. It is a colorless, odorless, highly poisonous gas. To prevent hazards, 1-2% chloropicrin (tear gas), a lachrymator, is added as a warning agent. The material is compressed as a liquid and is marketed in small metal cans or in large cylinders. A polyethylene sheet is immediately placed over the treated area during the soil injection process and allowed to remain for at least 48 hours, when soil temperature is about 152 C. If the temperature is below 152 C the exposure time should be extended to e n s u r e a d e q u a t e f u m i g a n t dispersion. Many California growers leave the polyethylene sheet on for a least 7 days and sometimes longer during cool weather of the winter. Chloropicrin (trichloronitromethane) is highly effective for the control of fungi and soil insects; provides good control of bacteria and nematodes but poor control of weeds. Large stocks of chloropicrin were left over from World War 1 and investigations showed the fungicidal, bactericidal and n e m a t i c i d a l p r o p e r t i e s of t h i s chemical. As with methyl bromide soil fumigations, a polyethylene sheet is immediately placed over the treated area during the soil injection process. The polyethylene sheet prevents damage from 117 Diseases and soil solutions drift of fumes to neighboring crops and hinderance to man. Soil fumigation with chloropicrin should not occur when soil temperatures are below 10aC. The treated land should be left undisturbed for a least two weeks during summer and at least three weeks in winter. If after these periods the fumigant still persists in the soil, as indicated by odor or eye irritation on close examination, the land should be disced or plowed to help aeration (Vanachter, 1979). Mixtures of methyl bromide/chloropicrin are commonly used in the United States for the control of soil pests. Chloropicrin and methyl bromide act synergistically. A combination of the two fumigants provides excellent control of many plant soil pests. Metam sodium (sodium-N-methyldithiocarbamate) provides excellent control of nematodes and good control of fungi, weeds and soil insects. It is sold in the United States under such trade names as Vapam or Soil-Prep. Metam-sodium is sold as a liquid which is soluble in water, producing a strong odor. Applied to the soil, it is converted into methylisothiocyanate (MIT). Metam sodium can be injected into the soil, applied by drenching, used in sprinkler systems or placed through drip irrigation lines. The variability of results with this compound has been a world-wide problem. Experi-ments continue in 1994 to develop the best method of applicatin for effective control. Dazomet (3,5-dimethyltetrahydro-1,3,5 (2H)-thiadiazine-2-thione) is sold as a finely granulated product under the trade name of Basamid. The chemical when in contact with moist soil hydrolyses to methylisothiocyanate. Basamid is not registered for use on any food crop in the United States at the present. Telone II (1,3-dichloropropene) is an excellent fumigant for the control of nema118 todes and provides good control of some soil insects. It is not effective for the control of fungi, bacteria or weeds. Telone C-17 contains 1,3-dichloropropene + chloropicrin and is effective for the control of nematodes, some soil pests and certain soil borne diseases. Telone II is not registered for use in California at the present time. Activity of fumigants in soil The important factors influencing the movement of soil fumigants are the chemical and adsorptive characteristics of the toxicant, t e m p e r a t u r e , moisture organic matter, soil texture and soil profile variability. Assuming the same soil profile and soil conditions at equivalent rate, the fastest and far reaching fumigants are, in o r d e r of activity, m e t h y l bromide, chloropicrin, 1,3-dichloropropene, and methylisothiocyanate, respectively. Soil fumigants, with the exception of MIT (methylisothiocyanate), are applied 10 to 90 cm below the soil surface as a liquid or gas. The first process that takes place in w a r m soils is t h e i n i t i a l vaporization of the chemical from liquid phase to the gaseous phase. The individual molecules now have an affinity for, and may be removed from, the pore spaces by the water, mineral, and organic phases of the soil. Since the diffusion of the gas is greater in air above the soil surface than with the soil system, upward mass flow and diffusion is greater than downward movement. Also, diffusion is unaffected by gravity. Therefore, seals of some sort are necessary at the soil surface to retain diffusing molecules long enough to give maximum effectiveness against organisms located near the soil surface (Munnecke, Van Gundy, 1979). The vapor pressure, or the rate at which the chemical leaves the liquid phase, is DR. important in determining the movement of fumigants in soil. If the vapor pressure of the liquid is high, as in the case of MB (methyl bromide), the gaseous concentration of the chemical in the soil pore spaces increases so rapidly that movement of the chemical occurs first by mass flow and then by diffusion. The vaporized molecules of soil fumigants tend to be dissolved in the soil water films. There is a continuous reestablishment of a dynamic equilibrium between air and water. This equilibrium is governed by Henry's Law, which states that the ratio of the concentration of the fumigant in the soil water to the concentration of the fumigant in the soil air is a constant at a given temperature. The vapor pressure and solubility of MB in water is high. If the soil water content for any fumigant is increased, both the rate of movement is slowed and the distance traveled in the soil pores are reduced. The characteristics of MIT are lower adsorption, relatively greater partition into water from air, slower diffusion, and higher decomposition rate at high temperatures, as compared with other soil fumigants. These factors probably are responsible for its relatively poor control of organisms in large soil masses. Solubility is important because most soil microorganisms are bathed in water films protecting them from direct contact with the fumigants in vapor phase. The toxic molecules must be present in the soil solution in which the target pest is bathed. Moisture is important in the adsorption of fumigant on soil particles. Soils at or dryer than the wilting point (-15 bars) have few or no water molecules surrounding the soil particles and adsorption of the fumigant molecules directly to the particle surface is increased. In moist soils, adsorption is reduced. In general, accepta- ALBERT O. PA ULUS ble toxicant movement is best in moist soils with a water potential of -0.6 to -15 bar. Polyethylene t a r p s of 25-100 pm thickness are used for MB and chloropicrin fumigations to slow loss of fumigants to the atmosphere. Compaction of the soil surface or the application of water seals are used with less volatile fumigants such as MIT. Usually, fumigant effectiveness increases with increase in temperature. Fumigants such as MB are not very effective in soils at temperatures below 10aC. A mixture containing 25,000 ul MB per liter of air was circulated over mycelial colonies of Pythium ultimum held for varying times at 5 temperatures between 52 and 30s C, inclusive. It required approximately 3.8 times more MB to kill 90% of the fungus propagules at 52C than at 302C. Soil fumigants are not always enhanced in warmer soil as compared to cold soils. Experiments in Georgia for the control of nematodes with vapam (SMDC), mylone (DMTT),l,3-dichloropropene and MIT when soil fumigated in the winter time concluded the best results were obtained when soil temperatures were below 162C, but above 02C. The reasons for this result were not known. Soil texture is often directly related to the success or failure of a field fumigation. Generally, coarse-textured soils (sandy loams) are easy to fumigate successfully, whereas fine-textured soils such as clay loams and clays, are more difficult to fumigate successfully. The soil texture on many agricultural soils varies with depth. Agricultural soils may have clay lenses at various depths, or they may be compacted below the surface. Either of these factors seriously affects downward diffusion of soil fumigants (Munnecke, Van Gundy, 1979). 119 Diseases and soil solutions Tomato soil fumigation trials Soil fumigation is a common practice in sandy soils repeatedly planted to tomato in Florida. Trials were initiated to compare various soil fumigants for the control of Fusarium wilt (Fusarium oxysporum f. sp. lycopersici) race 3, Fusarium crown rot, (Fusarium oxysporum f. sp. radicislycopersici), stingnematode (Belonolaimus longicaudatus), awl nematode (Dolichororus heterocephalus), stunt nematode (Tylenchorhynchus spp.) and root-knot nematodes (Meloidogyne incognita). Beds were constructed 20 cm high, 0.76 m wide on 1.4 m centers. Three streams of each chemical were injected 15 cm deep on a 20 cm center into 7.7 m single bed plots. Whitq/black laminated plastic fil (1.25 mil) was sealed over the bed as each plot was treated. Soil moisture (0-15 cm deep) at time of treatment in the fall and spring was 10.5% on a dry weight basis. This was roughly field capacity. Results are shown in Table 1. Table 1 Yield response of tomato to soil fumigants applied to Eau Gallie fine sand Yield (tons/1000 row ft.) Fusarium wilt Fusarium crown rot Treatment Rate/Acre Fall 1983 Methyl bromide 99.5% + chloropicrin 0.5% 400 lb. 5.33 a 4.67 a Methyl bromide 67% + chloropicrin 33% 350 lb. 4.79 ab 4.03 a 30 gal. 4.14 b 2.91 c 4.04 a 2.58 b Vorlex No treatment - (Vorlex-Methylisothiocyanate 20% + chlorinated C3 hydrocarbons 80% Significant 5% level Spring 1984 Methyl bromide 99.5% + chloropicrin 0.5% 400 lb. 5.09 a 4.72 a Vorlex 35 gal. 4.38 b 4.51 a Methyl isothiocyanate 40% (Trapex 40) 25 gal. 4.16 b 3.97 a - 2.12 c 2.72 b No treatment Significant 5% level DR. All treatments increased tomato fruit yield and reduced populations of sting, stunt, awl nematodes. Root-knot nematodes were suppressed by all treatments. All treatments reduced Fusarium wilt race 3 and Fusarium crown rot. A greater number of plants showed symptoms of Fusarium wilt at pH 5.5-6.0 than at pH 7.0-7.5; soil pH had no effect on incidence ALBERT O. PA ULUS of Fusarium crown rot (Overman, Jones, 1984). Jones and Overman in 1985 compared various soil fumigants for the control of root-knot nematode and Verticillium wilt (Verticillium dahliae) at soil pH of 5.5 and 7.5. Low and high soil pH plots were established by using sulfur or hydrated lime. Table 2 Effect of soil pH and soil fumigants on the percentage of "Sunny" tomato tomato plants with root knot and verticillium wilt Root-knot Verticillium wilt Treatment Rate/Acre at soil pH at soil pH 5.5 7.5 7.5 5.5 Percent Methyl bromide 67% 350 lb. 0 3 14 6 + chloropicrin 33% Methyl bromide 98% + chloropicrin 2% 300 lb. 1 7 6 Metam-sodium 50 gal. 32 87 7 25 gal. Vorlex 28 17 22 No treatment 46 93 17 (Metam sodium - 32.7% sodium N-methyldiothiocarbamate) 71 58 90 - The high pH encouraged development of v e r t i c i l l i u m w i l t a n d root-knot nematodes. Howewer, the injection of f u m i g a n t s into t h e s e high pH soils controlled Verticillium wilt and root-knot, resulting in maximum fruit production. In the same experiment raising the soil pH to 7.5 with hydrated lime in conjunction with the use of nitrate-nitrogen and mulch also greatly alleviated the severity of fusarium wilt, fusarium crown rot and southern blight, (Sclerotium rolfsii) and increased 0 yields (Jones, Overman, 1985). Corky root (brown root rot) has been recognized as an important disease of tomatoes in glasshouses in Europe for many years. Corky root also occurs on processing and fresh market tomatoes in scattered fields in northern and central California. The primary pathogen is Pyrenochaeta lycopersici which has also been recovered from nightshade (Solanum nigrum). Corky root severity declined as the transplanting date was delayed and 121 Diseases and soil solutions the soil became warmer in a trial by Campbell, et. al., involving plants inoculated in the greenhouse and transplanted to the field and noninoculated plants transplanted into infested soil. Yield loss estimates of up to 70% were made by growers of processsing tomatoes, and losses of the same magnitude occurred in fumigation experiments with fresh market tomatoes. A field trial in 1979 showed chloropicrin (168 kg/ha) and methyl bromide + chloropicrin (262 + 117 kg/ha) effectively reduced disease severity, increased plant size, and increased the yield of large fruits. Neither methyl bromide nor ethylene dibromide + chloropicrin reduced disease severity or increased yields significantly compared with nontreated check. In a separate trial metam sodium 935 1 of 33% metham sodium per hectare, was tested as a drench in the same field. Two treatments, with or without a 1 ml polyethylene tarp over the treated soil, were compared with the nontreated check. The plants in metam sodium treated soil were significantly larger (5.8 and 5.9 kg/plant without or with a tarp, respectively) than those in n o n t r e a t e d soil (3.9 kg/plant). The desease severity rating was reduced signifi-cantly to 0.4 by metam sodium with a tarp. Metam sodium without a tarp had a disease severity rating of 2.9, which was not significantly different from the nontreated check with a disease severity rating of 4.4. Fumigants selected for the 1980 plot were applied in October 1979. The chloropicrin and methyl bromide + chloropicrin fumigants and tarp, if specified, were applied by a commercial fumigation rig that treated and covered a 3.4-m-wide strip. The metam sodium was mixed with irrigation water as it flowed through a ditch onto each plot, where it was ponded by earthen dikes and allowed to percolate into the soil. Results are shown in Table 3 (Campbell, Schweers, Hall, 1982). Table 3 Comparison of four soil fumigants applied for control of Pyrenochaeta lycopersici in Greenfield sandy loam soil, Tulare county, CA in 1980 Treatment Methyl bromide 262 kg + chloropicrim 117 kg/ha Chloropicrim 168 kg/ha Chloropicrim 168 kgTia Metam sodium 935 1/ha Tarp Disease severity Yes 0.2 x Yes 0.5 x No 4.4 z No 1.9 y No treatment No 4.8 z Significant 5% level. Disease severity on five point scale. Ns large Plant + Fruit (kg) fruits/plant 9.8 x 25.1 wx 9.9 x 27.4 w 18.6 y 6.6 y 7.0 y 3.3 z 21.0 xy 7.3 z DR. ALBERT Control of strawberry pathogens In the field major requirements for successful soil fumigation are excellent, deep, fine soil tilth, somewhat less than seed bed moisture throughout the soil and soil temperatures of 139C and above. In addition, dosage is also a function of the degree of fungus infestation or inoculum potential of the soil, a fact commonly overlooked in commercial soil fumigation. Data from the following experiment illustrate the point. A field soil known to be heavily infested with Verticillium was diluted with steam soil in proportions of 1:1, 1:10 and 1:25. This established three different levels of Verticillium infestation in a uniform soil. The soil was placed in cylinders in the greenhouse, chloropicrin injected at rates of 2, 2.5 and 2 ml, respectively, a check series was added. 'Bonny Best' tomato seeds were planted in the treated soil. When the plants were 30 cm high, they were read for symptoms of wilt and cultured for Verticillium. The nonfumigated check soil gave a high infection index in dilutions of 1:1 and 1:10, and trace values in the 1:25 dilution; 1 ml of chloropicrin (160 lb/acre) gave poor control of Verticillium in the 1:1 soil dilution and excellent control in the 1:10 and 1:25 dilutions; 1.5 ml of chloropicrin (240 lb/ acre) gave excellent control of Verticillium except in the soil surface of the 1:1 soil dilution; 2.0ml (320 lb/acre) gave excellent control throughout all soil dilutions except for a trace in the surface soil of the 1:1 dilution. The data showed, and it has also been our experience in the field, that it requires more chloropicrin to achieve control of Verticillium in a heavily infested field t h a n in a lightly infested field. Thus, a required dosage is proportional to quantity of fungus inoculum (inoculum O. PAULUS potential) (Wilhelm, et. al, 1974). Chloropicrin and methyl bromide act synergistically. From field studies and observations we know that a dosage of 200 lb/acre of chloropicrin sealed by water or polyethylene film fails to control Verticillium wilt. The low r a t e may actually cause an increase in severity of the disease. Methyl bromide at a dosage of 200 lb/acre favors the disease. A mixture, however, composed of equal parts of chloropicrin and methyl bromide giving a rate of 200 lb/acre of each gives nearly complete control of the disease in a wide range of soil types and is one of the most effective fungicidal soil fumigants ever developed. This combination also controls other injurious soil fungi, such as Pythium, Phytophthora, Rhizoctonia, and Pyrenochaeta, and to soil-borne insects, nematodes and to both weed seed and dormant weed structure. Weeds of the genera Malva and Alfilaria, and of the common legumes, however, usually survive the fumigation (Wilhelm, et. al, 1974). Preplant soil fumigation allows us to plant on the same land year after year. Proportions of the fumigant components may be tailored to individual field requirements. Generally, the commercial practice on land to be p l a n t e d to strawberries and where Verticillium has been controlled by previous fumigation, is to use a 2:1 methyl bromide-chloropicrin mixture and to reduce the dosage where possible. In light soils the dosage may be reduced more than in heavy soils. Thus to control Verticillium wilt, 325-375 lb/acre of the 1:1 mixture is required; for fumigation of the same land for a subsequent planting of strawberries 275-375 lb/acre of the 2:1 mixture generally gives adequate results (Wilhelm, et. al., 1974). Tests compared chloropicrin alone and the mixture of chloropicrin, 2 parts, and 123 Diseases and soil solutions methyl bromide, 1 part, in a field application. One section of the experim e n t a l a r e a was irrigated prior to application of the fumigants and contained 12-15% of moisture a few inches belowthe soil surface, or a little under 50% of the estimated field capacity of the soil. An adjacent but smaller section was dry and cloddy. Applied to dry, cloddy soil, 480 lb/ acre of chloropicrin failed to control Verticillium wilt. In the moist area of the field the mixture of chloropicrin + methyl bromide provided excellent control of Verticillium at 320 lb/acre and covered with a polythene tarp (Wilhelm, et., al., 1961). Chloropicrin and metam-sodium were compared in England as a preplanting treatment for the control of Verticillium wilt of strawberry. Chloropicrin was injected at a depth of 15.2 cm at 30.4 cm staggered centers by a hand operated injector. Each injection delivered 3.6 ml (34.5 gal/acre) at the high dosage and 1.2 ml (11.5 gal/acre) at the lower. Plots were rolled twice and then sealed with water. Metam sodium was diluted with water and applied at 330 gal/acre and 110 gal/ acre by a watering can with a fine spray. The plots were rotavated after treatment. Results are shown in Table 4 (Talboys, Way, Bennett, 1966). Table 4 The effect of soil fumigation on the yield of Cambridge Vigour strawberry (means/plot) Treatment Total yield /lb) 1963 Total yield (lb) 1963 No treatment 29.1 107.8 Chloropicrin 34.5 gal/acre 34.9 135.6 Chloropicrin 11.5 gal/acre 29.1 135.6 Metham-sodium 330 gal/acre 29.8 126.2 Metham-sodium 110 gal/acre 32.8 119.2 S. E. mean 1.65 5.52 - Johnson et. al., compared full coverage vs. bed fumigation and compared Vorlex with methylbromide/chloropicrin soil fumigation in a field with fine sandy loam soil which had been fumigated and planted to strawberries the previous years. Soil treatments were applied in early August and plants were set in late August. The cultivar was Lassen. Treatments compared a mixture of 2 parts of methyl bro124 mide and 1 part of chloropicrin at 225 lb/ acre, Vorlex (20% methyl isothiocyanate + 80% chlorinated C3 hydrocarbons) at 30 gal/acre with full coverage applications and a bed aplication of methyl bromidechloropicrin at the rate of 225 lb/acre. All treatments were tarped for 24 hours. Results are shown in Table 5. (Johnson, Holland, Paulus, Wilhelm, 1962). DR. ALBERT O. PAULUS Table 5 Effect of fumigants and two methods of application on the yield of Lassen strawberries-Summer planting, Los Angeles county Number 12-pint trays/acre Treatment Gain over check Total yield Full coverage Methyl bromide 67% + chloropicrin 33%, 225 lb/accre 3,679 a 1,682 2,523 b 526 Methyl bromide 67% + chloropicrin 33%, 225 lb/acre 2,946 b 949 Check 1,997 c - Vorlex 30 gal/acre Bed application Significant 1% In England Harris compared chloropicrin and dazomet in soil fumigation experiments for the control of Verticilllium wilt of strawberry. Dazomet was applied during September and the chloropicrin applied in September/October. All plots were covered with a single sheet of polythene-nylon multilayer sheet of low gas permeability. Results are shown in Table 6. Table 6 The effects of pre-planting treatments on yield in the strawberry cv Hapil Dazomet Chloropicrin Control 450 kg/ha 210 l/ha Yield (g plant) 372 1,055 989 1987 541 1,038 999 1988 Chloropicrin was marginally more effective than dazomet for wilt control (Harris, 1989). Ridge fumigation was evaluated on soil to be planted to strawberries and infested with Verticillium dahliae as a cost-saving alternative to overall soil fumigation. A liquid fumigant injector was developed to be able to ridge-up and cover the ridge with polyethylene film in a single operation. Ridge fumigation treatments were applied in autumn, 1981, Diseases and soil solutions at bed formation, using different rates of both liquid and granular fumigants under black polyethylene film (p50m). Dazomet treatments were spread by hand just prior to ridging and incorporated into the ridged soil during ridge formation. The standard treatment for comparison was overall methyl bromide + chloropicrin soil fumi- gation with a mechanized rig which was done about 3 weeks before bed formation and covering with a black film. Strawberry plants cv. "Tioga" were planted through the film 5 weeks later. Fruit yields are shown in Table 7 for the 1981-82 and 1982-83 seasons. Table 7 Effect of mechanized ridge-only soil treatments on 'Tioga" strawberry yield for 1981-1982 and 1982-1983 seasons Total yield (t/ha) Application rate Treatment kg ai/ha 1981-1982 1982-1983 147 Chloropicrin 41.5 21.0 228 Dazomet 48.2 29.1 190 Dazomet 47.3 26.6 Methyl bromide + (overall) chloropicrin 450 47.6 m 28.8 Methyl bromide + (ridge) chloropicrin 138 38.3 16.1 24.1 4.3 7.1 4.7 Untreated - LSD 5% The lowest dazomet rate used was adequate for maximum response, whereas similar rates of methyl bromide/chloropicirin were too low. The benefits of ridge fumigation were not as great in a second season as they were in the first season. The trial area was heavily infested with Verticillium wilt inoculum, confirmed by the high level of disease that developed in u n t r e a t e d plots and the presence of Verticillium dahliae in these plants (Tate, Cheah, 1983). 126 Application of metam sodium Metam sodium is applied via t h e sprinkler irrigation systems to control Verticillium dahliae and other soilborne pathogens. The effectiveness of metam sodium in a trial in Israel for control of Verticillium throughout the top 40 cm soil l a y e r w a s i n f l u e n c e d by mode of application of the chemical (concentrate vs. dilute); type of soil, soil moisture, and the rate of water application. When applied DR. ALBERT to soils with medium or high clay content in the concentrated mode (dissolved in the first 10% of the irrigation water), the chemical killed Verticillium throughout a deeper soil profile than by dilute application (dissolved in the whole irrigation volume) especially in poorly structured soil in which water percolation is slow. In a clay soil (54% clay) with well defined aggregarated structure which enables quick water percolation, metam sodium O. PAULUS applied in a dilute mode killed Verticillium to a deeper layer than in a loessial soil (20% clay) with a single grained structure (slow water percolation). When metam sodium was applied in a concentrated mode, it was found to be important to irrigate a dry soil at a low rate of water application, in order to obtain uniform control throughout the treated soil profile (Ben-Yephet, Frank, 1989). 127 Diseases and soil solutions References BEN-YEPHET, Y . a n d FRANK, A . R . 1989. Factors affecting the efficiency of metham-sodium in controlling Verticillium dahliae. Acta Horticulturae 255:227-232. T. A. 1989. Useofsoilsterilantsin Florida vegetable production. Acta Horticulturae 255:61-72. BEWICK, R. N . , et. al. 1 9 8 2 . Corky root of tomato in California caused by Pyrenochaeta lycopersici and control by soil fumigation. Plant Disease CAMPBELL, 66:657-661. D. C. 1989. Control of verticillium wilt of strawberry in Britain by chem ical soil disinfestation. Jour. Hort. Sei. 64:683-686. HARRIS, et. al. 1 9 6 2 . Soil fumigation found essential for maximum strawberry yields in southern California. Calif. Agric. 1 6 : 4 - 6 . JOHNSON, H . , JONES, J . P. and OVERMAN, A . J . 1985. Management of Fusarium wilt, Fusarium crown rot, Verticillium wilt (race 2), southern blight, and root-knot of tomato on fine sandy soils. Proc. Fla. State. Hort. Soc. 9 8 : 2 2 9 - 2 3 1 . A. J . and J O N E S , J . P. 1984. Soil fumigants for control of nematodes, Fusarium wilt, and Fusarium crown rot of tomato. Proc. Fla. State Hort. Soc. 97:194-197. OVERMAN, TALBOYS, P . W . , W A Y , D . W . a n d BENNETT, M. 1966. Comparison of chloropicrin and metham sodium as pre-planting soil treatments for strawberry wilt (Verticillium dahliae Kleb) control. Plant Pathology 15:49-55. and C H E A H , L . H . 1983. Ridge fumigation for control of Verticillium wilt in strawberries. Proc. 36th N. Z. Weed and Pest Control Conf. 79-82. TATE, K . G . A. 1979. Fumigation against fungi. In Soil Disinfestation, Elsevier Scientific Publishing Company, p. 163183. VANACHTER, et. al. 1 9 7 4 . Preplant soil fumigation with methyl bromidechloropicrin mixtures for control of soilborne diseases of strawberries. A summary of fifteen years of development. Agric. and Environment, Elsevier Sei. Pub. Company, 2 2 7 - 2 3 6 . WILHELM, S . , et. al. 1 9 6 1 . Verticillium wilt of strawberry controlled by fumigation with chloropicrin and chloropicrinmethyl bromide mixtures. Phytopathology 5 1 : 7 4 4 - 7 4 8 . WILHELM, S . , M U N N E C K E , D . E . a n d VAN GUNDY, S . D. 1979. Movement of fumigants in soil, dosage responses, and differential effects. Ann. Rev. Phytopathol. 17:405-429. 128 DR. ALBERT Dr. Albert O. Paulus has been a member of the Plant Pathology Department, University of California, Riverside, California from 1954 to the present time. Obtained PhD in Plant Pathology from the University of Wisconsin, Madison, Wisconsin in 1954. Investigated diseases of vegetable, small fruit, ornamental and field crops and their control. Worked with Professor Wilhelm, Harold Lembright, and Richard Storkan in developing pre-plant soil fumigation for the control of plant pests. Named fellow of the American O. PAULUS Phytopathological Society in 1981. President, Pacific Division, American Phytopathological Society, 1986-1987. Keynote speaker at XIV Strawberry Symposium, Congreso Asociación International, Norcofel, Spain, 1988. P r e s e n t e d l e c t u r e s at Q u e e n s l a n d Agricultural College, Lawes, Australia, 1984. Research and extension leader on cucurbit diseases, USAID project between Egyptian and University of California scientists, 1982-1983. 129 Solarization in México D R . FLORENCIO JIMÉNEZ DÍAZ INIFAP DR. Introduction S o i l solarization is a technique which offers very promising chemical-free alternatives for controlling plant diseases caused by soil-borne microorganisms. Solarization is a term describing a technique which consists in trapping solar energy with clear polyethylene. Captured heat acts as a lethal agent which reduces soil-borne pathogen inoculation. Among the characteristics clear polyethylene has to offer are: its relatively low cost, excellent chemical resistance, hardness and flexibility; it's free of toxic substances and odors, and has low permeability to water condensation. These features are used to trap solar energy more efficiently. The main purpose of the following document is to show results reported all over the world, and those obtained in Mexico proving that this method offers effective soil-borne pathogen control, thus increasing yield and quality of the crops, besides prolonging the use of land available for agricultural production. Traditional ways of using heat to control disease Heat has been used to control plant diseases for years. The main variants FLORENCIO JIMÉNEZ DÍAZ reported are steam and hot water applied directly to the soil or vegetable material. An important bibliographical revision on the methods for soil disinfection with steam was carried out in 1955. The study pointed out the effectiveness of this method in eliminating all soil microorganisms (fungi, bacteria, actinomycetes, nematodes, etcetera) through overexposure to high temperatures. 46 Commercial m e t h o d s for soil sterilization with s t e a m have been described. This method requires 1802F temperatures at a constant rate during thirty minutes. 2 There is another soil treatment using steam and air and requiring lower temperatures which has the advantage of being selective in its extermination of plant pathogens, without destroying pathogen antagonists such as bacteria and actinomycetes. This has avoided recontamination risks when inducing an adequate biological control with the help of soilborne non-pathogenic microorganisms.5'49 There have been reports 3,7 - 33 ' 41,42,48 from several crops of the use of heat applied directly to vegetable tissue to obtain virus-free material. Other studies establish the elimination of bacteria, 17 micro-plasms, 36 nematodes, 16 and some other microorganisms through heat. 133 Solarization in México Use of plastics for plant disease control A reduction in the incidence of cabbage hernia has been associated with an increase in soil temperature due to solar radiation in a field covered with polyethylene. 69 Peanuts sown after a solarization period of six weeks resulted in a significant reduction of plants infected with Sclerotium rolfsii and weeds, together with a 52.8% and 123.5% increase in total yield and fruit quality, respectively. Plastic soil heating reduced Aspergillus spp. colonization in pods.20 The use of clear .03 mm (30 pm) thick polyethylene to cover a previously irrigated plot during the months of July through August has been consigned. In this study Verticillium dahliae was eliminated to a depth of 0-25 cm after two weeks of treatment. 34 Fusarium oxysporum f. sp. lycopersici populations were reduced 94 to 100% at a depth of 5 cm; 68 to 100% at a depth of 15 cm; and 54 to 63%, 25 cm below. Maximum temperatures on treated soils fluctuated between 49 and 52s C at a depth of 5 cm; reaching42 s C at 15 cm. Field experiments with eggplant and tomato reported a reduction in Verticillium, which fluctuated between 25 and 95%. Besides weeds were controlled and plant growth and yield were improved. In cases where there were temperature increases from 36 to 48SC in the top 5 cm of moist soil covered with polyethylene, the temperature ran between 44 and 522C on dry soil to the same depth. The temperature increased from 32 to 38SC, 4 to 20 cm deep on moist soil and from 35 to 392C on dry soil. Sclerotia from artificially inoculated Sclerotium oryzae last between 95-100% of their viability.24 134 The effectiveness of solar soil heating with clear polyethylene for controlling fung; in onion crops was also proved. Solar heating reduced the incidence and severity of pink rot caused by Pyrenochaeta terrestris 73-100% during the seven months following treatment, when the plants were growing. Rhizoctonia solani and Fusarium infections as well as weeds were also considerably reduced. 35 Excellent control of the Ditylenchus dipsaci nematode in garlic crops was achieved by covering the plot with clear polyethylene. Results were similar to those obtained with methyl bromide. 57 The effect of covering soil with clear polyethylene in the viability of plant pathogens was determined in an experiment with: Fusarium oxysporum, Pythium irregulare, Plasmodiophora brassicae, Sclerotium cepivorum, S. rolfsii, Sclerotinia minor, Verticillium dahliae and the Macropostonia xenoplax, Meloidogyne javanica, Pratylenchus penetrans and Tylenchulus semipenetrans nematodes. Preliminary tests showed that the pathogens died at temperatures within the 38 to 552C range. The most sensitive to the treatment were nematodes and V. dahliae, S. cepivorum andS. minor fungi, while F. oxysporum, P. irregulare and P. brassicae were the least sensitive. 51 Studies were carried out to determine the efficiency of soil solarization with clear polyethylene to control Verticillium dahliae and Pratylenchus thornei. The soil was irrigated and covered with 0.04 mm polyethylene for 31 days, during which it was kept moist. Potatoes were then planted on this treated soil. Solarization eliminated V. dahliaemicrosclerotia, reducing disease incidence 96 to 99%, and the P. thorrei population diminished 80-100%, weeds were also controlled, increasing yield 35% in contrast to the witness. 19 DR. The soil was covered with clear polyethylene to determine pathogen control. During the treatment the plot was irrigated with 5 to 10 cm of water. Temperatures were consistently higher in plastic covered soils than in uncovered ones and were lethal to many fungi. Rhizoctonia solani was eliminated from the 15 cm top layer of the soil after two weeks of treatment. Verticillium dahliae, Pythium spp. and Thielaviopsis basicola were essentially eradicated up to a depth of 46 cm covered during four weeks. 52 ' 53 An experiment in which moist soil was covered with clear polyethylene for a four and a half week period during the summer to evaluate the behavior of phytopathogens at high soil temperatures, found that populations of Agrobacterium, Pseudomonas, Gram positive bacteria and fungi were immediately reduced after treatment and remained significantly low after six and twelve months. 58 The effect of solarization on soil bacteria Four to five week solarization treatments applied during the summer reduced Agrobacterium species populations; an effect which lasted up to twelve months. In another experiment it was established that the percentage of Gram positive bacteria colonies exhibiting in vitro antibiosis against Geotrichum candidum increased close to twenty times more in solarized soil as compared to the witness. 58 60 The effect of solarization on phytoparasitical nematodes Total nematode populations of Pratylenchus protensis, Rotylenchus incultus, Criconemella xenoplas and mixed populations of Paratrichodorus lobatus plus P. FLORENCIO JIMÉNEZ DÍAZ minor-were reduced between 37 and 100% of their original levels through soil solarization. 6 When d e t e r m i n i n g t h e effect of solarization on Macroposthonia xenoplax, meloidogyne javanica, Pratylenchus penetrans and Tylenchulus semipenetrans nematodes it was found that these were more susceptible than common soil fungi under the conditions of this experiment. 5 Nematode control with soil solarization was compared with chemical control. Excellent results were obtained with clear plastic placed before planting. Chemical products initially reduced populations, which then increased considerably by the end of the crop.57 Field experiments for 4-6 weeks with solarization and/or nematocides, reported that with solarization and solarization + nematocide, reductions between 42-100% were achieved in t h e Meloidogyne, Heterodera, Pratylenchus, Paratrichodorus, Criconemella, Xiphinema and Paratylenchus populations in contrast to the witness. When only the nematocide was used, reductions were significantly lower.59 Effect of soil solarization on weed control Different solarization periods (from one to four weeks) were tested as was their effect on weed emergence. The841 grasses found in the witness were reduced to 9 in four week treatments. 300 other plants were found in the witness which were controlled 100% a f t e r 4 weeks of solarization. Morning glory was reduced from 76 in the witness to 2 in solarized plots; 100% purslane was found, and coquillo was unaffected by solarization. An investigation to compare clear and black polyethylene control over a series of 135 Solarization in México weeds found clear plastic offered better control and residual effects of up to eleven months. Different treatment periods were also tested and determined that after 6 weeks purslane and morning glory were eliminated 100% in counts carried out after three and twelve months, respectively.30 Solarization treatments lasting 10, 20, 30, 40 and 50 days for weed control concluded that longer solarization periods drastically reduced weed populations except in the case of fox tail. It was also observed that previously irrigated soils were better controlled than dry ones.43 Control of viral diseases with plastics Literature reports certain viruses t r a n s m i t t e d by aphids which attack cucurbitaceae in most producing parts of the world. 21 ' 44 - 47 Such is the case of watermelon mosaic virus 1 and 2, cucumber mosaic virus and squash yellow mosaic virus. Plastics have been used to control these diseases based upon the theory that reflecting covers confuse and disorient insects so they fly above and away from the mulch, instead of landing on the plant to feed.45 The effect of several colors of plastic mulch was determined for the percentage of squash showing symptoms of watermelon mosaic virus. The lowest percentage of sympto-matic fruit was observed in white plastic combined with oil.11 When testing different reflecting materials in order to measure the degree to which they repel vector insects (plant lice), and so reduce virus incidence in melons, the most outstanding treatment proved to be a clear plastic cover over 70% of the soil.43 136 Temperature and moisture changes in plastic covered soils When using 15 cm wide strips of polyethylene to increase soil temperature to a depth of 15 cm, it was observed that heat was retained at night. Temperature increases with black polyethylene were lower, but heat retention at night was higher. 63 Higher temperature increases have been reported for clear plastic than for black polyethylene. Soil moisture was slightly higher in covered soil than in uncovered soil.29 The effect of the width of the polyethylene strip on soil moisture retention was observed. Results show that at least 6 inches have to be covered in order to achieve a significant difference from the uncovered witness. 24 inch strips were superior to 6 inch ones.37 A model to forecast temperatures in polyethylene covered soils was developed. Significant temperature increases were proved in moist polyethylene covered soil. This is due, in the first place, to the fact t h a t evaporation is eliminated, and partially due to the greenhouse effect of the polyethylene cover. In the case of covered dry soil, the greenhouse effect dominates, thereby achieving a lower temperature increase. It has also been reported that heating on the edges of the plastic strip is lower than through the center, so a narrower strip is less efficient (heat-wise) than a wider one.39,40 Maximum moisture deviations occur in uncovered soils as opposed to polyethylene covered soils.32 Temperature increases of up to 10 to 12fiC in the top layers have been obtained in soil covered with clear polyethylene, as well as increased organic and mineral compound concentrations. 12 DR. Effect of temperatures on the development and viability of soil pathogens Phymatotricum omnivorum grows more rapidly at 28fiC. This fungus grows and produces sclerotia at a range of 15 at 352C, but maximum sclerotia yield is achieved at 282 C.38 Verticillium dahliae develops quicker at 25 +- 12C under lab conditions, while temperatures of 30 to 332C inhibit the formation of microsclerotia. 8 Laboratory experiments determined that temperatures between 37-502C were lethal to the mycelium, spores and microsclerotia of this pathogen. An exposition time of 28 days at 372C was considered the lethal dosis for this fungus. 64 Species of the Fusarium fungus have been recognized as warm climate. There are reports that the disease caused by this fungus progresses favorably under these conditions. In a (PDA) cultivation medium, the fungus grows at a rate of 9 at 37fiC. Under natural conditions it grew better at a rate of 24 at 312C. However, in the soil it didn't grow at temperatures over 342C or under 20SC.67,68 For some species of Phytopthora it has been reported that conidia form at a 91% relative humidity and is optimum at 100%, at a temperature range between 3 to 262C, the optimum being 18 to 222C. Optimum temperature for zoospores is 122C, and 252C for the formation of the sporangium germinative tubes. Zoospores germinate faster between 12 to 152C.67 Soil temperature, humidity and ventilation affect the survival and movement of most species of nematodes. It has been determined that, in general, a temperature of 202C and soil moisture content between 25 to 80% are f a v o r a b l e to t h e i r development. 1,31 FLORENCIO JIMÉNEZ DÍAZ Principles in soil treatment by heat Soil treatment by heat is among the oldest disease control methods, and has proven effective to control a great amount of pathogens. Nevertheless it's still underused. A list of principles on which soil treatment by heat is based, follows:4 1. Tolerance to high temperatures varies widely among living organisms. 2. Parasite microorganisms are more likely to die at lower temperatures than saprophytes. 3. Living matter is more prone to destruction by heat at higher moisture content in the soil. 4. The level of parasite propagule dormancy is directly related to their tolerance to heat. 5. The temperature required to kill a microorganism is directly related to the temperature range said organism has adapted to during its development. 6. Damage to microorganisms through h e a t t r e a t m e n t v a r y from delayed germination to partial growth and eventual or immediate death. 7. Parasite microorganisms die at temperatures only slightly damaging to hosts. Effect of soil solarization using clear polyethylene on plant behavior Field experiments have shown that plant growth benefits from polyethylene heating even in the absence of pathogens. Tomato plantules placed on samples of treated soil showed increased growth over plantules growing on non-treated soil.12 In experiments to determine the effect of solarization on soil nitrogen content, maximum tomato yield (29.8% ton/ha) was obtained with 60 kg/ha of N on treated soil, whereas in non-treated soil maximum 137 Solarization in México yield (25.8 ton/ha) required 138 kg^ha of N.32 Corn planted in soil previously treated with clear polyethylene grew faster, produced earlier and with higher yields than plants cultivated on non-treated soil or covered with black polyethylene. 29 Higher yields of garlic have been obtained from soils treated with clear polyethylene for pathogen control, than from those treated with methyl bromide and ethylene dibromide.57 Onion plant development and yield increased between 109 and 125% with clear polyethylene, with respect to the untreated witness. 35 Peach bushes planted on solarized soil showed a 25% increase in height and 42% in fresh weight; increases in height and fresh weight for Castile nut trees were 26 and 58%, respectively, when compared to those grown on untreated soils.58 Highest pepper yields were achieved from soils covered with clear and black plastic as opposed to other techniques. 23 Also, radish, pepper and cabbage yields were greater when cultivated on solarized soil.61 Solarization increased pepper yield 20%, whereas when the same plastic was painted and kept as mulch, yield was 53% higher than the witness. In the case of melon, a residual effect was detected on 138 yield when the fruit was planted the following spring. 25 Solarization studies practiced in Mexico In the eighties, preliminary work commenced in Mexico to determine the feasibility of using solarization in some areas of the country, where climate conditions, economical importance of the crops and complexity of phytopathological problems indicated the possibility of success. At the beginning of the investigation, the best solarization seasons were defined, as was the best period for solarization, its effect on soil pathogens and on crop yields, as well as its effectiveness in nematode and weed control. At present there's information from Irapuato, Apatzingan, Aguascalientes, Sinaloa, Torreon, Tamaulipas, Sonora and other places where problems caused by Fusarium spp, Rhizoctonia solani, Verticillium spp, Sclerotium cepivorum, Phytopthora spp, virosis, nematodes and weeds have been controlled in crops such as strawberry, melon, garlic, tomato, cotton, orchards, pepper, onion, watermelon, zucchini, cucumber, eggplant and others (Table 1). DR. FLORENCIO JIMÉNEZ DÍAZ Table 1 Place Irapuato Places and crops on which solarization research has been carried out in Mexico Problem Crop Strawberry Apatzingán Melon Aguascalientes Garlic Sinaloa Tomato Torreón Melon Cotton Orchards Pepper Onion Watermelon Zucchini Various crops (cucumber, eggplant, watermelon) Tamaulipas Sonora Others Fusarium, Rhizoctonia, Verticillium Weeds, nematodes Fusarium oxysporum f. sp melonis Macrophomina phaseolina Fusarium solani, virosis, weeds, nematodes Sclerotium cepivorum Weeds, nematodes Fusarium oxysporum f. sp radicilis Licopersici Weeds, nematodes Fusarium solani, Verticillium spp Phymathotrichum omnivorum Weeds, nematodes Phytophtora, Rhizoctonia, virosis Weeds Nematodes Weeds Soil-borne fungis Weeds Nematodes 139 Solarization in México Bibliography 1 1978. Plant Pathology. Academic Press. 703 pp. 2 BAKER, K . F . 1 9 5 7 . The U. C. System for producing healthy container grown plants. Calif. Agrie. Exp. Stn. Man 23. AGRIOS, N . G . 3 1962a. 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Enfermedades importantes de cultivos que se establecen en el Norte de México. G I F . C A E L A L A - Y. 1979. Control o/Sclerotium rolfsii and weeds in peanuts by solar heating of the soil. Plant Disease Reporter. 63(12) pp. 1056-1059. HERRERA P . CIAN-INIA-SARH. 29 GROGAN, R . G . , HALL, D . H . a n d KIMBLE, K. A. 1959. Cucurbit mosaic viruses in California. Phytopathology 4 9 : 3 6 6 376. 22 1982. 26 andELAD, 21 HAROON, S . M . a n d GHAFFAR, A . HARTZ, T . K . et al. 1 9 8 5 . Response of pepper and muskmelon to row solarization. Hort. Sc. 2 0 : 6 9 9 - 7 0 1 GRINSTEIN, A . , ORIONS, D . , GREENBERGER, A and KATAN, J. 1979. Solar heating of the soil for the control of Verticillium dahliae and Pratylenchus thornei in potatoes. Pages 431-438 in: B. Shippers and W. Gams Eds. Soilborne Plant Pathogens. Academic Press. London. 686 pp. HANKIN, L . et al. 1 9 8 2 . Effect of mulches on bacterial populations and enzyme activity in soil and vegetable yields. Plant and soil 6 4 : 1 9 3 - 2 0 1 . 25 CIANE-INIA-SARH. 19 DÍAZ 203-206. 1977. Estudio ecológico y distribución texana Phymatotrichum onmivorum Shear (Duggar) en los municipios de Cuauhtemoc a Gran Morelos. C A E S I C H - JIMÉNEZ Polyethylene mulching of soil to reduce viability of sclerotia of Sclerotium oryzae. Soil. Biol. Biochem. Vol. 14. pp. 1974. Association of a rickettsialike organism with Pierce's disease of grapevines and alfalfa dwarf and heat therapy of the disease in grapevines. Phytopathology 63(3) pp. 341-345. FLORENCIO Effects of black and transparent polyethylene mulches on soil temperature, sweet corn growth and maturity in a cool growing season. American Society for Horticultural Science. 8 6 : 4 1 5 - 4 2 0 . HOPEN, H . C . 1 9 6 5 . G R U P O INTERDISCIPLINARY DE FRUTICUL- 1982. Marco de Referenda del Cultivo de la Vid en la Comarca Lagunera. CIAN-INIA-SARH. TURA. 30 HOROWITZ, N . REGEV, Y . a n d HERZLINGER G. 1983. Solarization for weed control. Weed Science 3 1 : 1 7 0 - 1 7 9 . 141 Solarization 31 in México J E N K I N S , W . R . a n d TAYLOR, D . P . 1967. 39 Y . 1 9 7 9 . Prediction of soil temperatures of a soil mulched with transparent polyethylene. J . Appl. Meteorol 1 8 : 1 2 6 3 - 1 2 6 7 . 40 M A H R E R , Y . and KATAN, J . 1 9 8 1 . Spatial soil temperature regime under transparent polyethylene mulch: Numerical and Experimental Studies. Soil Science. PlantNematology. Reinhold Publishing Corporation. 270 pp. 32 MAHRER, J O N E S , T . L . , J O N E S . U . S . a n d EZELL, D. O. 1977. Effect of nitrogen and plastic mulch on properties of troup loamy san and on yield of Walter tomatoes. J. Amer. Soc. Hort. Sci. 102 ! (3): 2 3 7 - 2 7 5 . 131(2) 82-87. B. 1 9 5 4 . Heat-therapy of virus-infected plants. Ann. Appl. Biol. 33 KASSANIS, 41(3) pp. 41 MELLOR, F . C . a n d STALE, S . R . 1967. Eradication of potato virus X by thermotherapy. Phytopathology 5 7 : 6 7 4 - 470-474. 678. 34 KATAN, J . GREENBERGER, A . , A L O N , H. and G R I N S T E I N , A . 1 9 7 6 . Solar heating by polyethylene mulching for the control of disease caused by soil-borne pathogens. Phytopathology 66 pp. 683688. 35 42 MELLOR, F . C . a n d STALE S . R . 1976. Influence of heat therapy on rooting of, and elimination of raspberry bushy dwarf virus from shoot cuttings of red rasp-berry. Acta Horticulture: 66 pp. 63-70. KATAN, J . ROTEM, I . , FINKEL, U . and 1 9 8 0 . Solar heating of the soil for the control ofpink root and other soilborne diseases in onions. Phytoparasitica 8 ( 1 ) pp. 3 9 - 5 0 . DANIEL, J . 43 Avances de la investigación en el uso de plásticos en la producción de melón. INIFAPMUNRO, O . D . 1 9 8 7 . C I F A P M I C H - C E F A P V A . 23 pp. 36 KIKPATRICK, H. C., LOWE, S. K. and Peach Rosette: The morphology of an associated mycoplasm alike organism and the chemotherapy of the disease. Phytopathology 6 5 ( 8 ) . NYLAND, G . pp. 1975. 44 NAMETH, S . T . DODDS, J . A . a n d PAULUS, A. D. 1985. Zucchini yellow mosaic virus associated with sevedre diseases of melon and watermelon in Southern California Desert Valleys. Plant Disease 864-870. 69:785-788. 37 LIPPERT, L. F., TAKATORI, F. H. and Soil moisture under bands of petroleum and polyethylene mulches. American Society for Horticultural Science. 8 5 : 5 4 1 - 5 4 6 . WHITING, F . L . 1 9 6 4 . 38 142 S. D . 1 9 7 8 . Ecology of Phymatotrichum omnivorum. Ann. Rev. Phytopathology 1 6 : 1 9 3 - 2 0 9 . LYDA, 45 S . T. et al. 1 9 7 6 . Cucurbit viruses of California, and Ever-changing problem plant disease. 7 0 : 8 - 1 1 46 N E W H A L L , A . G . 1 9 5 5 . Disinfestation of soil by heat, flooding and fumigation. The Botanical Review. Vol. XXI No. 4 NAMETH, pp. 189-250 DR. 47 NELSON, M . R . a n d TUTTLE, D . M . 1 9 6 9 . 54 The epidemiology of cucumber mosaic and watermelon mosaic 2 of cantaloups in an arid climate. Phytopathology 49 NYLAND, G . 1 9 6 0 . Heat inactivation of stone fruit ringspot virus. Phytopathology 6 0 : 3 8 0 - 3 8 2 . OLSEN, C. M. Y BAKER, K. F. PULLMAN, G . S . , D E V A Y , J . E . , GARBER, R . RAMÍREZ, L. M . 1984. Priorización de problemas fitopatológicos radiculares del manzano. CAE Sierra de Chihuahua. Avances de Investigación Agrícola en Zonas de Riego y Temporal. CIANINIA-SARH. pp. 335-336. 56 SANDOVAL, B. J . 1984. Colección de cepas de Phytopthora capsici en chile, CAE Delicias. Avances de Investigación Agrícola en Zonas de Riego y Temporal. CIAN-INIA-SARH. pp. 86-87. 1968. PATRONATO PARA LA INVESTIGACIÓN, F O MENTO Y SANIDAD VEGETAL DE LA COMARCA LAGUNERA. Estadísticas de la producción agropecuaria y su valor. Ciclos 1982-1983 y 1983-1983. Zona de influencia de la Comarca Lagunera. 57 51 DÍAZ 55 Selective heat treatment of soil, and effect on the inhibition of Rhizoctonia solani by Bacillus subtilis. Phytopathology 5 8 : 7 9 - 8 7 . 50 JIMÉNEZ H. and W I N H O L D , A. R. 1981. Soil solarization and thermal death: A logarithmic relationship between time and temperature for four soilborne plant pathogens. Phytopathology 71(9) 959964. 59:849-856. 48 FLORENCIO SITI, E . , COHN, E . , KATAN, J . a n d M O R - 1982. Control o/Ditylenchus dipsaci in garlic by bulb and soil treatments. Phytopatasitica 10(2) pp. 93-100. PORTER, I . J . a n d MERRIMAN, P . P . 1 9 8 2 . DECHAI, M . Effects of solarization of soil on nematode and fungal pathogens at two sites in Victoria. Soil. Biol. Biochem. 15(1) pp. 39-44. 58 52 H . and WINHOLD. A. R . 1979. Control of soil-borne fungal pathogens by plastic tarping of soil. Pages 439-446, in: B. Shippers and W. Gams Eds. Soilborne Plant Pathogens. Academic Press. London. 686 pp. 53 59 STAPLETON, J . J . a n d D E V A Y , J . E . 1 9 8 3 . Response of phytoparasitic and freeliving nematodes to soil solarization and 1,3-Dichloroprapere in California. Phytopathology 73:1429-1437. PULLMAN, G . S . , D E V A Y , J . E . , GARBER, R . H . and W I N H O L D , A . R. 1981. Soil solarization: Effects on Verticillium wilt of cotton an soilborne populations of Verticillium dahliae, Pythium, spp., Rhizoctonia solani and Thielaviospsis basicola. Phytopathology 71(9) pp. 954959. STAPLETON, J . J . a n d D E V A Y , J . E . 1 9 8 2 . Effect ofsoil solarization on populations ofselected soilborne m icrooganisms and growth of deciduous fruit tree seedling. Phytopathology 71(3) 323-326. PULLMAN, G . S . , D E V A Y , J . E . GARBER, R . 60 STAPLETON, J . J . a n d D E V A Y , J . E . 1 9 8 4 . Termal components of soil solarization as relates to changes in soil and root microflora and increased plant growth response. Phytopathology 74:255-259. 143 Solarization 61 62 in México andDEVAY, J . E. 1985. Soil solarization: Effects on soil properties, crop fertilization and plant growth. Soil Biol. Biochem 17:369-373. de Meloidogyne sp sobre cinco especies hortícolas destinadas a producción de semilla y evaluación de dos nematicidas para su control. Informe Anual de Labores CIAN-INIA-SARH. STAPLETON, J . J . , QUICK, J . STREETS, R . B . a n d BLOSS, H . E . 1973. Phymatottorichum root rot. Monograph N 2 8. American Phytopathological Society, pp. 38. 63 TAKATORI, F. H., LIPPERT, L. F. 64 65 F. L. G. P. y Ruiz DE LA R. J. DE D. 1978. Poblaciones de nemátodos fitoparásitos detectados en viñedos de la Comarca Lagunera. Informe Anual de Labores. CIAN-INIA-SARH. (En proceso de publicación). 67 WALKER, 68 J. C. 1971. Fusarium wilt of tomato. Monograph N2 6. The American Phytopathological Society. 56 pp. 69 W H I T E , J . G . a n d BUCZACKI, S . T . and The effect of petroleum mulch and polyethylene films on soil temperature and plant growth. American Society for Horticultural Science. 8 5 : 5 3 2 - 5 4 0 . WHITING, 66 1964. D. 1982. La vid en México. Datos Estadísticos. Colegio de Postgraduados. 321 pp. i TELIZ, O . G. P. y Ruiz DE LA R. J. DE D. 1976. Comprobación de patogenicidad VALLE, VALLE, J. C. 1969. Plant Pathology. McGraw-Hill Book Company. WALKER, 1979. Observations on suppression of clubroot by artificial or natural heating of soil. Trans. Br. Mycol. Soc. 73(2). pp. 271-275. Drip irrigation and microspraying in citrics and vines B E N A M I BRAVDO Agricultural Faculty BEN AMI Abstract J 3 o t h drip and microsprinkler irrigation a r e included in t h e category of microirrigation namely, only part of the soil and the root system are irrigated. Methods consisting of partial wetting of the root system require more frequent water application since in this case the soil serves as a water reservoir only to a limited extent. Microirrigation methods enable, however better control of the root e n v i r o n m e n t with respect to water availability, mineral concentration in the root zone and aeration. Drip is a more e x t r e m e form of microirrigation as compared to microsprinklers, since the irrigated soil volume in this case is smaller. The use of soil tensiometers in drip systems irrigated at constant frequent irrigations enables to maintain a constant soil water potential in the root zone and thereby irrigate at the rate of consumptive use by the trees. Results of irrigation experiments BRAVDO in citrus showed that root restriction, created by the combination of one lateral and high fertilizer concentration induced a high rate of fruit bud differentiation and consequently increased number of fruits per tree. The increased fertilizer concentra-tion helped to prevent an overcropping situation and the large number of fruits, without reduction in fruit size, resulted in a constant high production over the entire four experimental years. Drip is the dominant method of irrigation in vineyards in the western world. The combination of high water availability and a e r a t i o n e n a b l e s to obtain high productivity, large berry size and advanced ripening. In winegrapes, the control of vegetative growth at various stages of fruit ripening is essential for improving quality. Experimental evidences show that correct use of drip fertigation enables such a control and optimization of crop load, as well as fruit composition relative to sugar, acid and aroma compounds is obtained. Intensification and conduction of tomato cultivation D R . RODRIGUE N E L S O N VIGOUROUX Agro Industrias Campus DR. ^ ^ e g e t a b l e production in greenhouses has increased in Mexico. These greenhouses are mostly dedicated to tomato production, which is why I'd like to speak about our experiences in this field. A greenhouse is a work utensil which must conform to a biological and economical framework. Some of the elements determining its location are environmental radioactivity, climate, water, soil and labor. The quality of the plant in the greenhouse is very important, since production depends upon it. It is precisely in the greenhouse where the future of the crop (and especially of the harvest) is prepared. General aspects of plant production a) A good plant is hard to define, however its development must take into account production goals and the biological particularities of the crop. 1. Production goals A well developed plant is that one capable of producing the highest yield with the best quality (shape, size, etc.). This young plant is where the future of the crop lies. As a matter of fact, it is during this period when the formation of RODRIGUE NELSON VIGOUROUX cotyledons takes place, up until the development of the second and third leaf when the first blooming starts. Light, temperature and nutrition are the three elements that determine the formation of the plantule. After the third leaf to blooming, the environment must not vary since a deficiency in any of the elements (light, temperature, water and nutrition) could result in flower abortion. The quality of a plant can be judged by certain visible characteristics, such as a healthy appearance in all its parts, turgescence and richness in dry matter, smooth cotyledons. When transplanted, it should be in a state such that will allow it to withstand the environmental conditions. Plants should be homogeneous. Plantules must be prepared in a greenhouse (we'll call it a multiplication greenhouse) dedicated to that purpose. A good separation between stalks can be achieved by allowing 20 pots per m2. The greenhouse must be equipped with movable tables and adequate heating, independent from external climate conditions. Seed It is advisable to submit perfectly healthy seeds to thermo-therapy; a 60 s C treatment results in disinfected seeds. 151 and conduction of tomato cultivation Cultivation m e d i u m Fertilization Seeds can be sown in a humid medium, that is also parasite-free, poor in salts, slightly acid, enriched with nutrient solution, etcetera. It is a good idea to cover seeds with 1 to 1.5 cm (about .5 in) of substratum during germination. Air temperature of the substratum is kept steady at 20s C. The strongest plants are selected once cotyledons are horizontally well developed, in other words, 10 to 12 days after sowing. Malformed plants are discarded. Its very important that cotyledons are in good shape since the young plant totally depends upon them. Transplant will take place when the first blooming starts. Excess nitrogen should be avoided in plantules. It should also be noted that their absorption of phosphoric acid is inhibited by low temperatures combined with lack of light. Potassium partially compensates lack of light. C h a r a c t e r i s t i c of s u b s t r a t u m 1. High water retention. 2. Well ventilated; i.e. porous. 3. Allows good root development since its not hardened. 4. Healthy or parasite-free. 5. pHfi. 6. Does not contain excess nutrients. 7. Can be soaked with 2 kg of fertilizer per m3, in order to enrich it with phosphorus and potassium. Temperatures The temperature of the substratum has a bearing on the growth of the roots as well as water and nutrients. Growth is inhibited at temperatures under 142 C; while phosphorus absorption decreases 50% at temperatures between 18 and 12° C. During the preparation phase of the plant, temperatures act directly upon final yield in fruit size. 152 1. T r i m m i n g of s e c o n d a r y s t e m s Typically, t h e main stem of an indeterminate variety reveals shoots three leaves before producing flowers (or clusters of flowers), followed by three leaves, cluster... etcetera. The axil of every leaf produces a shoot which develops a secondary s t e m . Trimming consists in eliminating these shoots to give the plant a more ventilated structure, concentrating its strength to producing larger fruits. a) T r i m m i n g to a guide: All axillar shoots are either broken off manually or cut off with a knife after they've grown 5 to 10 cm. All secondary shoots —but not the main s t e m - are eliminated. Among the advantages of conducting to a guide are: - greater production precocity, - higher production, - larger size, and - greater homogeneity. 2. T e r m i n a l t r i m m i n g Indeterminate varieties of tomatoes can continue growing indeterminately. Depending upon the type of crop and environmental conditions, a decrease in the size of the fruits can be observed in the higher branches, after the sixth, seventh DR. or eighth cluster. In certain cases, and depending upon desired results, the top of the plant (the terminal shoot) can be eliminated; a procedure which stems growth. Trimming the plant to 6-7-8 clusters (on the same stem) results in: - fruits plumping out more rapidly, - reaching maturity sooner, - greater caliber (size and quality), and - reduced cultivation period. The cluster forms a thorn. If more fruits occur in a bifurcated cluster, it's because floral induction has taken place under stress: cold, salinity, etcetera. The terminal flowers of a cluster can be eliminated to favor plumper fruits, leaving for example, only five fruits to mature identically to the same seize. 4. Eliminating leaves Leaves aren't to be eliminated until they're exhausted. A symptom of this is that they become white (loss of magnesium) and brittle (easily crushed). Anyway, these leaves should appear under the cluster being harvested. If symptoms of exhaustion show up in the upper leaves, this means that there is a nutrition problem. It should be noted that fruits nourish themselves from the reserves of the leaves. An indiscriminate elimination of these results in accelerated maturity, decreased size, and loss of color. RODRIGUE NELSON VIGOUROUX soil and the quality of irrigation water. The following information is a mere guide and should be adapted to each separate case. In general it's important to keep conductivity in the irrigation water after adding the fertilizer. A good rule of the thumb is not to exceed 1 gram of fertilizer for every liter of water irrigated. Fertilizers which leave saline residues in the soil (sulfates, chlorides, etcetera) must be avoided. Its not advisable to supply Mg with P or with the K, since this can result in precipitations or interferences. 1. Fertilization balances a) Seed beds and post-planting; until the second cluster: N-P-K; 15-30-15 b) From third cluster until the start of harvest: N-P-K 15-15-30 c) From the start of harvest until the trimming of the plant: N-P-K 15-10-30 d) From the trimming to the end of harvest: N-P-K 20-0-30 Magnesium is supplied separately from the other fertilizers, preferably in the form of magnesium nitrate (or as magnesium) at a rate of one part for every five parts of the potassium supplied. 2. Irrigation III. Fertilizers Instructions as far as balancing the fertilizers to be used and watering the plants are relative and provided only as guidelines. The amounts to be supplied depend upon atmospheric conditions, observations of plant responses, type of Irrigation varies greatly according to the cycle of the crop: a) Greenhouse sowing/Spring for spring/summer harvest. b) Summer sowing for fall/winter harvest. The maximum supply necessary in a 153 crop where the harvest and fructification phases are to take place in mid-summer would be 1-2 liters of water per plant a day, at that moment. V. How to increase the size of the fruit Some practices promote greater fruit size: - Using water with a low salinity level. - Forcing nitrogenous fertilizer; a N/K ratio of 1/1.5 or 1/2 during the plumping phase affords interesting results. - Trimming to a guide. - Trimming after the sixth or seventh cluster. - Trimming the cluster to leave a limited amount of fruits. VI. Training Due to the cultivation period, training is particularly recommended for indeterminate type tomatoes. Training helps keep the plant straight and well ventilated; it sustains the weight during the long production and harvesting period benefiting the quality of the fruit. Traditional supports or trainers can be easily modified with higher trainers (1.5 m to 1.8 m) to maximize yields from an indeterminate plant. When plants are fixed to a guide, the number of plants can increase to 25 thousand per hectare. Depending upon the composition and structure of the land, a single central drip line is used; in parcels with a high degree of filtration a drip line in every plantation row is used. Carbon dioxide C0 2 The idea of applying CO., is to increase 154 its concentration thus stimulating better plant development, increase in yield, better harvest quality and earlier blooming and fruit growth. In most cases of indoor crops, atmospheric concentrations of CO, have always limited p l a n t growth, since t h e s e atmospheric concentrations can sometimes decrease to levels below 180 ppm, while normal levels rem between 300 to 350 ppm. Benefits of applying CO, - Pure C0 2 (99.5%) is available. Its use avoids burning fuels which produce toxic gases noxious to plants, such as sulphur (S) by-products, nitrogen oxides (NOx), and others. - Profit increases exceed several times the cost of the treatment and initial investment. - E q u i p m e n t for controlling and calculating CO, concentrations is simple and reasonably priced. - Besides it has been proven that modern techniques, such as the use of plastics in agriculture, drip irrigation, soil mulching, microtunnels and greenhouses allow direct application of C0 2 on leaves. - C0 2 prolongs tissue life. - It also prolongs the productive life of the plant, thus avoiding excessive tilling; saving great amounts of plastic, drip tapes, etcetera, and labor which, translates into lower costs and greater profits. CO., application technique is based on the following: 1. CO, gas can be mixed with irrigation water. 2. This mix is applied through the drip irrigation system 3. C0 2 will spontaneously escape from the soil into the atmosphere, a process enhanced by changes in temperatures, DR. pH, etcetera. 4. C0 2 application becomes even more adequate when drip irrigation is combined with soil mulching. RODRIGUE NELSON VIGOUROUX 5. Optimum applications are carried out in greenhouses 6. C0 2 gas can be applied in the irrigation hoses. 155 MEMORIAS se terminó de imprimir el 18 de abril de 1994, en los talleres fotolitográficos de Impre-Jal, impresores de Guadalajara, Jalisco, México, en Nicolás Romero 518. La edición fue de 1,000 ejemplares, y estuvo cuidada por el licenciado Alfonso Ñuño.