Cell Proliferation - Bio

Cell Proliferation
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Bio-Connect Diagnostics B.V.
Begonialaan 3a
6851 TE Huissen
The Netherlands
T NL +31 (0)26 326 44 60
T BE +32 (0)2 502 12 53
F NL +31 (0)26 326 44 61
F BE +32 (0)2 502 12 77
E [email protected]
W www.bio-connectdiagnostics.nl
CELL PROLIFERATION &
CYTOTOXICITY ASSAY - EZ4U
.
EASY hANdLINg
.
CONvENIENT
.
FAST
.
NON-TOXIC
.
NON-RAdIOACTIvE
.
RELIAbLE
.
SENSITIvE
EZ4U
bI-5000 EZ4U CELL PROLIFERATION ANd CYTOTOXICITY ASSAY
ASSAY ChARACTERISTICS
Proliferation assays are widely used in cell biology for the study of growth
factors, cytokines, nutrients and for the screening of cytotoxic or chemotherapeutic agents. The EZ4U cell proliferation and cytotoxicity assay
Method
is based on the capability of living cells to reduce sIightly coloured or
Reduction of tetrazolium salt to
coloured formazan
uncoloured tetrazolium salts in the mitochondria into intensely coloured
formazan derivates. This water soluble formazan is secreted into the
Sample type
cell culture medium
Sample size
200 µl / test, 10x96 tests
Detection limit
depending on cell lines
Incubation time
2-5h
culture medium and can be measured with a standard colorimetric reader.
The EZ4U system is well suited for a variety of biological tests, where cell
viability is of importance. It offers advantages over conventional dye or 3H
incorporation assays. Due to its soluble end products it is easier and faster
to perform than other non-radioactive cell viability assays. Furthermore,
because the assay procedure is identical to the Thymidine incorporation
procedure, no changes in test protocols are necessary. An important
benefit is the possibility of an ongoing cultivation after cell number determination and easy to adapt incubation times due to the non-toxic substrate.
EZ4U
MTT
ThYMIdINE
ADD SUbSTRATE
REMOvE SUPERnATAnT
ADD THyMIDInE
InCUbATE 2-4 h
ADD SUbSTRATE
InCUbATE 6-36 h
ELISA READER
InCUbATE 4 h
HARvEST DRy
FILTERS
ADD
SOLUbILISER
PLACE FILTERS
In vIALS
InCUbATE
1h
ADD SCInTILLATIOn
COCKTAIL
MIx by
PIPETTInG
COUnT In
bETA-COUnTER
LITERATURE
Influence of harvesting technique and donor site location on in vitro growth
of osteoblastlike cells from facial bone.
Pradel W. et al., Int. J. Oral Maxillofac. Implants 2005, 20(6):860-866
Significant growth inhibition of orthotopic pancreatic ductal adenocarcinoma by CpG oligonucleotides in immunodeficient mice.
Tepel J. et al., Int. J. Colorectal Dis. 2006, 21(4):365-372
The effect of selective sst1, sst2, sst5 somatostatin receptors agonists,
a somatostatin/dopamine (SST/DA) chimera and bromocriptine on the
„clinically non-functioning“ pituitary adenomas in vitro.
Gruszka A. et al., Life Sci. 2006, 78(7):689-693
EZ4U-E-300306
In vitro activity of cycloSal-nucleoside monophosphates and polyhydroxycarboxylates against orthopoxviruses.
Sauerbrei A. et al., Antiviral Res. 2005, 67(3):147-154
Effects of met-enkephalin on cell proliferation in different models of
adrenocortical-cell growth.
Malendowicz L.K. et al., Int. J. Mol. Med. 2005, 15(5):841-845
ELISA READER