bergman

bergman
October - December 2015
Issue 4
Contents
Our new website . . . . . . . . . . . . . . . . . page 2
Water purification . . . . . . . . . . . . . . . . page 3
Rotavapor R-300 . . . . . . . . . . . . . . . . . . page 4
Lab reactor . . . . . . . . . . . . . . . . . . . . . . page 5
Centrifuge . . . . . . . . . . . . . . . . . . . . . . . page 5
DNA/RNA UV-cleaner box . . . . . . . . page 5
DNA/RNA analysis . . . . . . . . . . . . . . . page 6
DNA purification kits . . . . . . . . . . . . . page 6
DNA extraction kit . . . . . . . . . . . . . . . . . page 6
NanoDrop One . . . . . . . . . . . . . . . . . . page 7
Media preparation . . . . . . . . . . . . . . . . page 7
transOMIC . . . . . . . . . . . . . . . . . . . . . . page 8
New products from NEB . . . . . . . . . page 8
FlashGel . . . . . . . . . . . . . . . . . . . . . . . page 10
New antibodies from CST . . . . . . . page 10
Primary cells . . . . . . . . . . . . . . . . . . . page 11
Antibody production . . . . . . . . . . . . page 12
Cell death kit . . . . . . . . . . . . . . . . . . . . . page 12
Cytation 5 . . . . . . . . . . . . . . . . . . . . . . . page 13
Plate washers and readers. . . . . . . page 15
Our service team . . . . . . . . . . . . . . . page 16
BioNordikaBergman AS • tel 23 03 58 00 • fax 23 03 58 01 • [email protected] • www.bionordikabergman.no
NEW WEBSITE!
www.bionordikabergman.no
BioNordikaBergman is proud to finally introduce to you our new webpage. With this page, we provide you with
information about our consumables, research products and instruments, all one site.
What is new?
• Webshop!
You may set up a new user account and thus get access to prices and order entry.
And if you need primer or probes our oligonucleotide order form is still available.
• Product search!
Search for specific products and get easy access to all the alternatives that we offer from our
different suppliers.
• Easy access to special offers, news and activities!
As a distributor of several suppliers we offer a huge product portfolio, and our new website will only give you a
small overview of what we can offer. For more product details, you can follow links directly to our suppliers. If you
can’t find your favorite product, please do not hesitate to contact us by e-mail or phone.
2
Water purification
Purelab® flex 3 - The most intuitive and innovative system for all your pure water needs
The PURELAB flex is uniquely designed around your needs. It combines ease of use with the accurate delivery of pure water. The
flexible modular system can be configured to deliver the quality of water you need for your applications.
Features
• Intuitive to use, ergonomic handset design
• Clear water purity display for absolute confidence as you dispense
•Real-time TOC monitoring for critical analytical applications
•Prioritized information shown at all times
(system status, TOC, alarm)
•Point of use filters for multiple applications
• Flexible dispensing by:
- Variable flow - drop by drop or up to 2 liters per minute
- AutoVolume dispense with 50ml to 60 liters and
repeat dispensing
- Hands free with optional foot pedal
- Locked dispense for glassware filling
Specifications Flex 3
• Daily volume: <10 liters
• Dimensions: (W) 236mm (D) 470mm (H) 900mm (max. height: 102 cm)
•Operational Weight: 23 kg
• Feedwater pressure (max): 90psi (6 bar)
(If <60psi (4 bar) a separate boost pump is required)
• Mains Input: 100-240V ac, 50-60Hz
PURELAB Chorus - In Tune with your Needs
The PURELAB Chorus gives you the chance to configure a solution that best suits your needs with the added benefit of flexibility
should your future needs change. Ideal for a wide range of applications including sophisticated research and testing applications,
qualitative analyses, feeding ultrapure water systems, glassware washing, autoclaves etc. The list of possible applications is
endless!
Featuring:
• PURELAB Chorus 1:
ultrapure water for critical
applications
• PURELAB Chorus 2:
for your pure Type II+/II solutions
• PURELAB Chorus 3:
for your general grade
Type III solutions
• Halo Dispense Solutions:
three remote dispensing and
monitoring solutions
• Storage Reservoirs:
designed to accomodate
PURELAB Chorus water
purification systems
Do you want to learn more about our products for Water Purification?
Contact Monica Laukas at 404 40 960
[email protected]
3
!
NEW
Rotavapor ® R-300
More convenient. More effective. More for you.
∙
∙
∙
∙
Control all process parameters with a central interface
Automate your individual SOPs using configurable methods
Distill even foaming samples without supervision
Receive push notifications on your mobile device when a process is finished
www.buchi.com/rotavapor
Quality in your hands
The R-300 meets the highest expectations in convenience and versatility. Its modular design allows extension of the Rotavapor®
R-300 to a fully integrated system where a central interface regulates each component.
Your most important benefits:
Convenient
• Comfortable flask and vapor duct removal thanks to easy to use Combi-Clip
• User-friendly operation due to ergonomic electric or manual lift
• Immediate adjustment of heating temperature and rotation speed with intuitively positioned control knobs
Extendable and versatile
•Easy plug & play extension to a fully integrated system including a central interface, vacuum pump and recirculating chiller
according to your needs
• Choice between 5 L high-temperature and 1 L fast-heating bath
• Wide range of beneficial accessories available: seven different glass assemblies, safety shield, lid for heating bath, automatic
distillation and automatic foam detection
Reproducible
•Precise operation control with integrated digital display of heating temperature, rotation speed and lift position
• Traceability of all process parameters including data recording and charting with the optional Interface I-300 Pro
• Instant alert with push notification on mobile devices in case of process deviation (with Interface I-300 / I-300 Pro only)
Introduction offer: Prices from Kr. 32 000,Do you want to learn more about Rotavapors?
Contact Marte Tøften at 922 11 099
[email protected]
4
the Reactor-Ready™ Lab ReaCtor
Reactor-Ready is designed as a universal reactor work station with a range of easily interchangeable vessels from 100ml to 5 litres
which can be configured to suit the chemistry and scale needed for each project. Easy to use, this one unique framework can replace many, saving money and fumehood space.
Reactor Features
•Rapid, tool-free vessel exchange with quick-release vessel clamp and hose couplings.
•Accepts all leading brands of overhead stirrer and allows easy, tool-free adjustment.
•Triple support stand features heavy duty stainless steel support rods for stability.
•Self-aligning stirrer coupling engages without the need for tools.
•Innovative hose manifolds allow easy thermofluid drain down.
•Temperature range: -70ºC to +230ºC.
•Wide range of accessory glassware including condensers, dropping funnels etc.
•Optional software allows you to log and control stirrers, circulators, balances, pumps,
temperature sensors and other devices.
Reaction Vessel Features
•Range of single and vacuum jacketed vessels from 100ml to 5 litres.
•Process vessels to mimic larger scale plant or manufacturing reactors.
•Precision engineered borosilicate glass vessels.
•DN100 5 neck Glass Lid fits all Vessel sizes.
•Vessels feature V4 Zero Dead Space Drain Stopcock which prevents over-tightening which
can cause glass breakage.
Do you want to learn more about Lab Reactors?
Contact Marte Tøften at 922 11 099
[email protected]
LAB EQUIPMENT FROM BIOSAN
NEW! Centrifuge/Vortex for PCR plates
CVP-2 (cat.no. BS-010219-A02) is a fully automatic device for reproducing The
Spin–Mix–Spin technology for 2 PCR plates at the same time, thus saving time
considerably. The Spin–Mix–Spin technology is intended to spin-down micro
volumes of reagents on the well’s bottom (first centrifugation spin), following
mixing (mix) and spin-down the reagents again from the walls and cap of the
well (second spin). This repetitive algorithm of operation is aimed to reduce
the mistakes during sample preparation for PCR analysis.
A must-have instrument for PCR and DNA analyses laboratory.
Includes rotor for 2 PCR plates, adapters for semiskirted and unskirted PCR-plates.
-20 %
DNA/RNA Uv-cleaner box
UV-cleaner boxes are equipped with an open UV lamp installed in the
upper hood. UV-radiation from the open lamps disinfects the working
area inactivating DNA/RNA fragments during 15–30 min of exposure.
A digital timer controls duration of the direct UV irradiation. A daylight
lamp provides proper illumination of the working surface.
UV-cleaner boxes are equipped with a flow-type bactericidal UV cleanerrecirculator AR, which provides constant decontamination inside the
box during operation. They are recommended for operations with DNA/
RNA amplicons.
NOW!
We offer 20% discount on all DNA/RNA UV Cleaner Boxes
from Biosan!
Do you want to learn more?
Contact Kristin Lundemo at 992 27 101
[email protected]
5
Eurogentec NEWS!
SMARTPURE
The SmartPure Kits are easy-to-use kits intended
for quick and efficient minipurification of DNA
fragments and plasmid DNA. They contain a SmartPure column made of a silica based membrane
which separates efficiently the DNA molecules from
the reaction mixture.With the SmartPure kits the
isolated DNA can be used immediately in all your
routine molecular biology applications.
The SmartPure Plasmid Kit
Provides high quality purified plasmid DNA. The
plasmid isolated from bacterial cell culture can
be used directly in any downstream molecular
biology applications.
Working time: +/- 20 minutes. Typical yield: Up to
40 μg plasmid DNA / 1-5 mL E.coli culture*
*Depending on the plasmid copy number and the culture OD.
SMARTPURE BENEFITS
• Easy to use:
Each kit is provided with a Smart Protocol card
•Fast:
Purification
completed in a
few minutes
•Efficient:
High recovery yield
•Convenient:
100 Preps pack size and stable for
18 months
The SmartPure PCR Kit
It is perfectly suited to efficiently separate DNA fragments from PCR or enzymatic
reaction mixtures without using toxic phenol and chloroform solvants.
Working time: +/- 6 minutes. Typical yield: From 60 bp to 10 kbp.
Elution recovery: ≥99%
Ladder
of success
The Shimadzu concept of
DNA/RNA analysis is faster
and more sensitive than ever
before.
Providing an inexpensive
alternative to agarose gel
electrophoresis, the
Shimadzu MultiNA system
is the ladder of success to a
new level of electrophoresis
analysis simplifying the daily
routine, e.g. in forensics and
clinical diagnostics.
• High sensitivity detection
• High resolution and
reproducibility
The SmartPure Gel Kit
A convenient kit for the extraction and purification of DNA fragments from up to 3%
standard (or high/low melting) agarose gel in either TAE or TBE buffer.
Working time: +/- 16 minutes. Typical yield: From 60 bp to 23 kbp
Elution recovery: ≥99%
Cat. no.
Product
SK-PLPU-100
SmartPure Plasmid kit, 100 preps
INTRODUCTION PRICE
539,-
SK-PCPU-100
SmartPure PCR kit, 100 preps
539,-
SK-GEPU-100
SmartPure Gel kit, 100 preps
539,-
SMARTFLEX BENEFITS
SMARTEXTRACT
The SmartExtract DNA Extraction kit achieves
genomic DNA extraction from almost any crude
sample type (e.g. blood, human & animal tissues,
plant seeds & leaves...). DNA extraction is
performed in a single tube in 12 minutes only.
The recovered solution can be used directly in any
amplification reaction (PCR or end point qPCR).
•Fully-automated
fast analysis
Cat. no.
Product
• Direct documentation
of results
SK-DNEX-100
SmartExtract DNA
kit , 100 preps
INTRO.
PRICE
887,-
Do you have questions about DNA
extraction and purification?
Contact Gitika at 940 07 207
[email protected]
6
• Easy to use:
Extraction in 1 tube
only, with no
precipitation,
no column
•Fast:
Extraction completed
in 12 minutes
•Efficient:
Ideal for rapid
genotyping
•Universal:
A single kit for
various sample
types
•Convenient:
Stable at room
temperature, and
compatible with robotics
NEW! Move one step closer to success with NanoDrop One
Quantify and qualify DNA, RNA, and protein samples with only 1–2 μL in seconds with no dilutions using the Thermo Scientific™
NanoDrop™ One microvolume UV-Vis spectrophotometers. Gain a more complete understanding of sample quality before using
samples in downstream applications with Thermo Scientific™ Acclaro™ Sample Intelligence technology built into every NanoDrop
One instrument.
• Fast and easy evaluation of nucleic
acids and proteins with Auto Measure and pre-programmed
applications
• Modern standalone design with
local control and high-resolution
touchscreen interface saves
bench space
• Accurate measurements up to
27,500 ng/μL (dsDNA) with
extended auto-range pathlength
technology
• No consumables needed –
pipette directly onto the pedestal
sample-retention system
• Enhanced connectivity with data
transfer via USB, Ethernet,
Bluetooth® and Wi-Fi options;
PC software available for data
management
• Optional cuvette position for
measuring dilute solutions and
performing temperature
sensitive experiments
NOTE!
Information regarding NanoDrop 1000
All service and support on NanoDrop 1000 will end in December 2015. Unfortunately there will be no more new software
releases or all hardware support.
Do you want to learn more about NanoDrop?
Contact Kristin Lundemo at 992 27 101
[email protected]
Do you need aquipment for media preparation?
We offer petri dish fillers and media preparators from Integra, and
can advice you to find the best solution for your laboratory!
Do you want to learn more?
Contact Monica Laukas at 404 40 960
[email protected]
7
NEB NEWS!
Rapid PNGase F
PNGase F is the most effective enzymatic method
for removing N-linked oligosaccharides from glycoproteins. PNGase F digestion deaminates the
asparagine residue to aspartic acid, and leaves the
oligosaccharide intact for further analysis. Rapid
PNGase F is an improved reagent that allows the
complete and rapid deglycosylation of antibodies
and immunoglobulin fusion proteins, as well as other
glycoproteins, in minutes. All N-glycans are released
rapidly and without bias, and are ready to be
prepared for downstream chromotography or mass
spectrometry analysis.
Cat. no.
Product
P0710S
Rapid PNGase F
Other Glycobiology Reagents Now Available
α1-2,4,6 Fucosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-2, α1-4
and α1-6 linked l-fucopyranosyl residues from oligosaccharides.
α1-3,4,6 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-3, α1-4 and
α1-6 linked d-galactopyranosyl residues from oligosaccharides.
Cat. no.
Product
P0748S/L
α1-2,4,6 Fucosidase
P0747S/L
α1-3,4,6 Galactosidase
New Restriction Enzymes
The new restriction enzymes from NEB are all active
in the CutSmart® Buffer.
Visit www.neb.com to view the full list of restriction
enzymes available from NEB.
8
Cat. no.
Product
R3575S/L
BsrGI-HF®
R0680S/L
BssSαI
R0667S/L
BtsαI
R3198S/L
MluI-HF
R3192S/L
NruI-HF
R3127S/L
NsiI-HF
and more NEB NEWS!....
NEB® Golden Gate Assembly Mix
The efficient and seamless assembly of DNA fragments, commonly referred to as Golden Gate assembly, has its origins in
1996 when, for the first time, it was shown that multiple inserts
could be assembled into a vector backbone using only the
sequential or simultaneous activities of a single type IIS
restriction enzyme and T4 DNA Ligase. This method can be
accomplished using Type IIS restriction enzymes, such as BsaI,
and can also be used for the cloning of single inserts. The
method is efficient and can be completed in one tube in as little
as 5 minutes for single inserts, or can utilize cycling steps for
multiple inserts. Golden Gate Assembly has been widely used
in the construction of custom-specific TALENs for in vivo gene
editing, among other applications.
Cat. no.
Product
E1600S
NEB Golden Gate Assembly Mix
In its simplest form, Golden Gate Assembly requires a Type IIS
recognition site, in this case, BsaI (GGTCTC), added to both ends of
a dsDNA fragment. After digestion, these sites are left behind, with
each fragment bearing the designed 4-base overhangs that direct
the assembly.
HiScribe™ T7 ARCA mRNA Kits
Most eukaryotic mRNAs require a 7-methyl guanosine (m7G)
cap structure at the 5 ́ end and a poly(A) tail at the 3 ́end to
be efficiently translated. The HiScribe T7 ARCA mRNA Synthesis
Kit (NEB #E2060) is designed to synthesize capped and tailed
mRNAs for variety of applications. Capped mRNAs are
synthesized by co-transcriptional incorporation of Anti-Reverse
Cap Analog (ARCA) using T7 RNA Polymerase. A poly(A) tail is
then added by E. coli Poly(A) Polymerase. A separate version
of the kit, without E. coli Poly(A) Polymerase (NEB #E2065), is
available for use with DNA templates encoding a poly(A) stretch
or not requiring a poly(A) tail. The kits also include DNase I and
LiCl for DNA template removal and quick mRNA purification.
Cat. no.
Product
E2060S
HiScribe T7 ARCA mRNA Kit (with tailing)
E2065S
Hiscribe T7 ARCA mRNA Kit
Both cap and tail are required for mRNA function in cell culture
Luciferase expression in U2OS cells. Purified Cypridina luciferase
RNA produced as indicated was co-transfected into U2OS cells
with purified Gaussia luciferase mRNA. mRNAs produced using the
HiScribe T7 ARCA mRNA Kit (with Tailing) are 5 ́-capped and have
3 ́ poly(A) tails. After 16 hours incubation at 37°C, cell culture
supernatants from each well were assayed for CLuc and GLuc
activity. Luminescence values were recorded and used to calculate
relative luciferase activity.
NEBNext Library Quant Kit for Illumina®
Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each
sequencing run. Furthermore, for Illumina sequencing, accurate quantitation of libraries is critical to achieve optimal cluster
densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate and effective method of library
quantitation, providing considerably higher consistency and reproducibility of quantitation. Amplification-based methods
quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the
concentration of the library molecules that can be sequenced. The NEBNext Library Quant Kit delivers significant improvements to
qPCR-based library quantitation for next gen sequencing.
Cat. no.
Product
E7630S/L
NEBNext Library Quant Kit for Illumina
Do you have questions about
products from TransOMIC and NEB?
Contact Gitika Emberland at 940 07 207
[email protected]
9
FlashGel Device Pack - Gel Electrophoresis and Gel Documentation in one unit
Complete separation, recovery and documentation safely, at the bench, in minutes.
With the FlashGel™ System, you get great resolution and fast results. Simply
load samples, watch bands migrate and get data in as little as 2 minutes.
Say goodbye to gel preparation, band excision, purification, and UV light.
Experience the Speed of the FlashGel™ System
• 5 minute separation and recovery
• Excellent band resolution
• Precast, prestained agarose gels and buffer
- no need for gel preparation, buffer addition
or gel staining
The FlashGel™ Device Pack is the easy way to speed up
your DNA and RNAseparation and recovery. All the
equipment you need, in one part number.
Cat. no.
Product
Special offer
57062
FlashGel™ Device Pack
16 500,-
(Incl. FlashGel™ Dock, FlashGel™ Power Supply
and FlashGel™ Camera)
Do you have questions about FlashGel?
Contact Gitika Emberland at 940 07 207
[email protected]
BUY 3 PRODUCTS AND STEM cell and deveolpment targets
GET A 4TH FREE
Use Code EU15SLS When Placing Order
Oct-4A (D6C8T) Rabbit mAb (mouse specific) #83932
Marker of stem cell pluripotency.
WB, IP, IF, ChIP and Flow Cytometry validated.
Confocal IF using #83932 (green) and
Phalloidin #13054 (red).
BUY 3 GET 4th
FREE!
Place an order with Cell Signaling Technology (CST) for 3 or more products
using the promotion code EU15SLS, and receive a 4th product free.*
Place an order of any 3 or
more products from Cell
YOUR CST DiSTRiBUTOR:
BioNordikaBergman AS
Signaling Technology
Gladengveien 3B, 0661 Oslo
Tel: 23 03 58 00
using the promotion code
Faks: 23 03 58 01
E-post: [email protected]
EU15SLS, and receive a 4th
product free.*
If you would like to know more about this promotion or our product listings, please contact your local distributor.
*Offer only valid in AT, CH, CZ, DE, DK, ES, FN, FR, IT, NO, PL, PT, SE, TR. Expires December 31st, 2015. No cash or cash equivalent. No substitutions. Offer valid
on new orders only. Offer may not be applied to existing, pending or prior orders. Promotion code EU15SLS must be referenced when placing order. Offer applies to
purchases of 3 catalog items or more and excludes petite (40µl) or smaller sized products. Free item must be of equal or lesser value to the lowest priced purchased
item. Cannot be combined with any other promotions. Limit 1 promotion per order. Items that are not eligible, tax and shipping and handling charges will not be included
in determining merchandise subtotal.
For Research Use Only. Not For Use In Diagnostic Procedures.
© 2015 Cell Signaling Technology, Inc. Cell Signaling Technology and CST are trademarks of Cell Signaling Technology, Inc.
15FLYCORPPRDI0016ENG_02
*Promotion code EU15SLS must
be referenced when placing order.
Offer applies to purchases of 3 catalog
items or more and excludes peptide
(40 ul) or smaller sized products.
Free item must be of equal or lesser
value to the lowest priced item.
New Sox Family Rabbit mAbs for IHC and IF
Sox 9 rabbit mAb #82630
WB, IHC and IF validated.
Sox10 rabbit mAb #89356
WB, IP and IF validated.
IHC analysis using #82630.
PROX1 (DsJ6J) Rabbit mAb #14963
Transcription factor that plays critical roles in organ
development as well as EMT in certain cancers.
WB and IHC validated.
IHC analysis using #14963.
Do you want to learn more?
Contact Kristin Lundemo at 992 27 101
[email protected]
10
BioResearch
Clonetics™ Primary Cells and Media
The Trusted Path to Relevant Results
30
th
Anniversary
Clonetics™
Primary Cells
Choose Lonza for Optimal Cell Performance
Lonza’s Clonetics™ Primary Cells and Media are globally renowned for
quality and reliability. As the pioneers of primary cell culture, Lonza
celebrates 30 years of providing these products and expertise to
scientists for consistent and biologically relevant results every time.
Our breadth of cell-type offering is unmatched and spans across research
areas such as respiratory, neurobiology, cancer, stem cells, diabetes,
cardiovascular and others.
© 2014 Lonza Walkersville, Inc
Clonetics™ Primary Cells Portfolio:
–
–
–
–
100+ cell types – cryopreserved or proliferating, human and animal
Broad donor variety – large selection of donors for most cell types
Cells from normal and diseased tissues
Optimized Growth Media BulletKit™ for each cell type
The trusted path to results starts with Lonza.
www.lonza.com/primary
11
Reference
SPEEDY PTM
Antigen = Peptide
produced by Eurogentec
1 modified (PTM)
1 unmodified
Peptide design
Included
Peptide synthesis
>70%
10-13 aa
15-20 mg (modified)
10-20 mg (unmodified)
Peptide coupling to carrier
Coupling of 5 mg peptide
Carrier
KLH, BSA, OVA or THY
28-Day Program
Yes
Rabbits
2
Elisa Guarantee
Optional
Affinity purification + ELISA
Double purification of 50 ml serium
ELISA
Aditional services
Optional
Shipment on dry ice
Yes
Delivery
Purified Antibody
Remaining Serum
Remaining Peptide
Speedy 28-Day
Your Polyclonals in 28 days only!
Speedy 28-Day is the reference program for the
production of custom polyclonal antibodies
at Eurogentec. This proprietary program uses
a non-Freund adjuvant that stimulates the
immune response and gives mostly IgG’s,
despite the short immunization time.
Speedy Post Translational Modification
Speedy 28-Day includes a very efficient
immunization protocol with frequent boosts.
It is therefore well adapted to generate Abs,
specifically targeting PTMs, as it can minimize
instability of the modification(s) harbored on
the peptide used as antigen.
By far the most popular requested PTMs
specific Abs are directed to phosphorylated
residues but we can also do Anti-Methylation,
Anti-Acetylation and many more PTM Abs.
Do you want to learn more about Custom Antibody Production?
Contact Målfrid Røe at 975 90 405
[email protected]
Highly specific and sensitive nanoimmunoassay of PARP1
A toolkit to measure cell death
Background
PARP1 is the primary member of the poly(ADP-ribose) polymerase family, whose function is to signal DNA damage (and to
recruit repair proteins) by PARylation. PARP1 is also involved in
multiple cell death pathways, including apoptosis, necroptosis,
autophagy, and a relatively new pathway termed parthanatos.
It has been implicated in a new form of cell death termed
parthanatos. PARP1 can also promote tissue survival by shifting
the balance of cell death programs between autophagy and
necrosis. Clinical studies have shown vulnerability to PARP
inhibitors in DNA repair defective cancers. Anti-PARP1 (internal)
antibody is useful for researchers interested in cellular processes
including DNA damage, transcriptional control, and stem cell
identity research.
Application Note
Anti-PARP1 (internal) antibody with the matched secondary
has been validated by western blotting and nanoimmunoassay
(NIA). Specific conditions for reactivity should be optimized
by the end user. Expect a band approximately 113 kDa in size
corresponding to PARP-1 by western blotting in the appropriate
cell lysate or extract.
Questions?
Contact Kristin Lundemo at 992 27 101
[email protected]
12
PARP1 (internal) Antibody Combo Pack Properties
Cat. no.
K-X51
Host
Rabbit
Known Cross Reactivity
Human
Clonality
Polyclonal
Application
Western Blot : 1:500 - 1:2000
Kit Type
Combo Pack
Cytation™
5 is a5 uniquely
integrated,
configurable
system
that
Cytation™
is a uniquely
integrated,
configurable
system
that
combines
automated
digital
widefield
microscopy
with
conventional
combines
automated
digital
widefield
microscopy
with
conventional
Cytation™
5microplate
is amicroplate
uniquely
integrated,
configurable
system
that
multi-mode
detection
to provide
phenotypic
cellular
multi-mode
detection
to
provide
phenotypic
cellular
combines
automated
digital
widefield
microscopy
with
conventional
information
and
well-based
quantitative
data.
This
instrument
information
well-based
quantitative
data. Thissystem
instrument
Cytation™
5 is aand
uniquely
integrated,
configurable
that
multi-mode
microplate
detection
tosoftware
provide
phenotypic
replaces
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modules
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instrument
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up
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up
to
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magnification,
the
microscopy
in
fluorescence,
brightfield,
H&E
stain
and
phase
contrast
channels.
in fluorescence,
brightfield,
stain and
phase contrast
channels.
replaces
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software
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yet is simple
to
module
provides
high-quality
cellular
and
sub-cellular
visualization
The
multi-mode
detection
module
features
BioTek’s
patented
The
multi-mode
detection
module
features
BioTek’s
patented
setup and operate. With up to 60x magnification, the microscopy
in fluorescence,
brightfield,
H&E
stain andvariable
phase
contrast
channels.
Hybrid
Technology™,
which
incorporates
bandwidth
Hybrid
Technology™,
which
incorporates
variable
bandwidth
module provides high-quality cellular and sub-cellular visualization
The multi-mode detection module features BioTek’s patented
in fluorescence, brightfield, H&E stain and phase contrast channels.
Hybrid Technology™, which incorporates variable bandwidth
The multi-mode detection module features BioTek’s patented
Hybrid Technology™, which incorporates variable bandwidth
monochromator
optics
monochromator
opticsand
andhigh
highsensitivity
sensitivityfilter-based
filter-baseddetection
detection
optics
forfor
unmatched
optics
unmatchedversatility
versatilityand
andperformance.
performance. Temperature
Temperature
monochromator
optics
and high
sensitivity
detection
control
toto
6565
°C°C
and
plus
available
CO
and
control
andshaking,
shaking,
plus
availablefilter-based
CO2/O
/O22control
control
and
2
optics
for
unmatched
versatility
and
performance.
Temperature
dual
reagent
injectors
optimize
conditions
for
cell-based
imaging
dual
reagent injectors
optimize
conditions
for filter-based
cell-based imaging
monochromator
optics
and high
sensitivity
detection
control
to 65 °CImage
and
shaking,
plus
available
COand
/O2powerful
control
and
and
detection.
capture,
data
collection
image
2and
and
detection.
Image
capture,
data
collection
powerful
image
optics
for unmatched
versatility
and
performance.
Temperature
dual
reagent
injectors
optimize
conditions
forsoftware,
cell-based
imaging
and
data
analysis
are
managed
with
Gen5™
specifically
and
data
analysis
are
managed
with
Gen5™
software,
specifically
control to 65 °C and shaking, plus available CO2/O2 control and
and
detection.
Image capture,
data collection
and
powerful
image
designed
forfor
uncomplicated
processing
ofofeven
most
designed
uncomplicated
processing
even
the
mostcomplex
complex
dual reagent
injectors optimize
conditions
forthe
cell-based
imaging
and
data
analysis
are
managed
with
Gen5™
software,
specifically
assays.
assays.
and detection. Image capture, data collection and powerful image
designed for uncomplicated processing of even the most complex
and data analysis are managed with Gen5™ software, specifically
assays.
designed for uncomplicated processing of even the most complex
assays.
Euglena
gracilis,
Euglena
gracilis,
60x 60x
Zebrafish,
Zebrafish,
4x 4x
TiliaStem,
TiliaStem,
2x2x
Z-stack,20x
20x
Z-stack,
Euglena gracilis, 60x
Zebrafish, 4x
TiliaStem, 2x
Z-stack, 20x
Euglena gracilis, 60x
Zebrafish, 4x
TiliaStem, 2x
Features:
Features:
Z-stack, 20x
• Fluorescence, brightfield, H&E stain and phase contrast
•Features:
Fluorescence, brightfield, H&E stain and phase contrast
microscopy and conventional multi-mode detection
microscopy and conventional multi-mode detection
• • Fluorescence,
brightfield,
H&E stain
and phasedetection
contrast
Patented Hybrid
Technology™
in multi-mode
• Patented
Hybrid
Technology™
in multi-mode
detection
microscopy
and
detection
combines
highconventional
performance multi-mode
filters
with variable
bandwidth
combines high performance
filters
with
variable bandwidth
for versatility
and
performance
• monochromators
Fluorescence, brightfield,
H&E
stain
and phase contrast
• Patented
Hybrid Technology™
in multi-mode
detection
monochromators
for versatility and
performance
microscopy and conventional multi-mode detection
combines
high
performance
filters
with
variable
bandwidth
• Variable bandwidth fluorescence monochromators
offer a 9 nm to
• Variable
bandwidth
fluorescence
monochromators
offer a 9 nm to
monochromators
for
versatility
and
performance
50
nm
in
1
nm
increments
for
ultimate
flexibility
• Patented Hybrid Technology™ in multi-mode detection
50 nm in 1 nm increments for ultimate flexibility
combines
high performance
filters
with
variableoffer
bandwidth
®
®
• •Variable
bandwidth
fluorescence
monochromators
a 9 nm to
Laser-based
excitation
for AlphaScreen
/ AlphaLISA
assays
®
®
monochromators
for
versatility
and
performance
• Laser-based
excitation
for
AlphaScreen
/
AlphaLISA
assays
50 nm in 1 nm increments for ultimate flexibility
• CO2/O2 control, incubation to 65 0C and shaking optimize cellVariable
fluorescence
monochromators
offer
a 9 nm to
®shaking optimize
•• •CO
/O2 control,
incubation
to 65 0C and
cellbased
andbandwidth
other
assays
Laser-based
excitation
for
AlphaScreen
/ AlphaLISA® assays
2
50 nm
1 nmassays
increments for ultimate flexibility
based
andinother
0
Low
(2incubation
µL) nucleic to
acid
protein
quantification
with the
• •CO
/Ovolume
control,
65and
C and
shaking
optimize cell2
2
®
®
Laser-based
excitation
for
AlphaScreen
/
AlphaLISA
assays
• •Low
volume
(2
µL)
nucleic
acid
and
protein
quantification
with
the
available
Take3
Plates
based and other assays
available Take3 Plates
0
Powerful
yet
to use Gen5
software
forquantification
efficient plate
reading,
CO
/O2 control,
incubation
65protein
C and
shaking
optimize
cell• ••Low
volume
(2 easy
µL) nucleic
acid to
and
with
the
2
image
and
analysis
• Powerful
yet
to assays
use
Gen5 software for efficient plate reading,
basedcapture
andeasy
other
available
Take3
Plates
image capture and analysis
Low volume
(2 to
µL)use
nucleic
and protein
quantification
with the
• •Powerful
yet easy
Gen5acid
software
for efficient
plate reading,
available
Take3
image
capture
andPlates
analysis
Features:
Cytation 5 with dual injector (left) and
/O2 Gas Controller (right)
2
CytationCO
5 with
dual injector (left) and
CO2/O2 Gas Controller (right)
Cytation 5 with dual injector (left) and
CO2/O2 Gas Controller (right)
Cytation 5 with dual injector (left) and
CO2/O2 Gas Controller (right)
•
Powerful yet easy to use Gen5 software for efficient plate reading,
image capture and analysis
www.biotek.com
Scan with your smart phone to watch the video.
Scan with your smart phone to watch the video.
www.biotek.com
Scan with your smart phone to watch the video.
www.biotek.com
Scan with your smart phone to watch the video.
Do you want to learn more about Cytation™ 5?
Contact Hanne Ramstad at 414 79 591
[email protected]
www.biotek.com
13
14
ELx50 strip washer
•
•
•
•
•
•
•
•
•
Washes 96- and 384-well microplates
Magnetic and polystyrene bead assays
ELISAs and cell-based assays
Filtration-to-waste processes
Patented Dual-Action™ wash manifold for independent control of dispense and aspiration tubes
Comprehensive onboard software makes programming quick
and easy
Automatic switching for up to three wash buffers
Programmable shaking duration and intensity
Built-in maintenance programs
Epoch & Take3 nanodrop plate
A microplate reader and a nanodrop in one instrument
•
•
•
•
•
•
•
•
•
•
Save valuable sample by using Take3 to quantitate
2 μL volumes of DNA, RNA and proteins
Monitor cell growth using the Epoch’s well scanning capability
Run spectral scans of 2 μL samples, or samples
contained in a microplate or cuvette
Dial in individual wavelengths from 200 – 999 nm
Perform rapid qualitative or quantitative analysis in microplates
from 6- to 384-wells
Measure kinetic rates of reaction
Run multiplex assays quickly and easily
Save space on the benchtop with its very small footprint
Avoid costly maintenance with the robust, thoughtfully
engineered optics and hardware
Never purchase costly absorbance filters
MultiFlo FX Dispenser/Washer
•
•
•
•
•
•
•
Up to four independent reagents dispensed in parallel with no potential for carryover
Unique Parallel Dispense technologies – choice of
peristaltic or syringe pumps for unique assay requirements
Large, high resolution color touch screen display
Onboard computer with two USB flash drive ports for file transfer, storage and operation
Optional plate washing module increases functionality
Modular and upgradable
ELISAs, cell-based assays (including automated staining and fixing for imaging) and bead-based assays such as Luminex
xMAP®
Do you want to learn more?
Contact Hanne Ramstad at 414 79 591
[email protected]
15
OUR
SERVICE TEAM
When did you last
service your
spectrophotometer?
As a supplier of equipment and
instruments to laboratories
throughout the country, we do
know how important it is that
everything is working optimal.
Our skilled service engineers
has all a high degree of
competence within their product
range and their expertise is kept
at the highest level with
continues trainings and courses
at the suppliers.
Should your plate
reader have been
calibrated?
Maintenance of instruments is
crucial to make sure that they
will give accurate and reliable
results. Our service team follow
the suppliers’ guidelines during
the maintenance assignment.
Many of our customer have
signed a periodic maintenance
agreement that ensures our
follow up and assistance.
These agreements can be custom
tailored depending on the
equipment/instruments in
your lab.
Should the filters
in your water
purification system
have been changed?
After a maintenance assignment,
we set up a detailed report with
information about the condition
of the instrument, replaced spare
parts and things that might need
to be improved.
In addition to periodic
maintenance, we also perform
installations, repairs, calibrations
and other technical assistance,
either in our service facility in
Gladengveien 3B or at your lab.
Maybe we can assist
you with this or
something else?
Promo code: NL-4/15
bergman
Note! The promo code is required to obtain the special prices!
The offers are valid until 31.12.2015 and cannot be combined with other
offers or agreements. The prices are listed excl. VAT and shipping charges.
We reserve the right to typos
BioNordikaBergman AS • tel 23 03 58 00 • fax 23 03 58 01 • [email protected] • www.bionordikabergman.no