bergman

bergman
April - August 2016
Issue 2
Contents
TOC-L . . . . . . . . . . . . . . . . . . . . . . . . . . . page 2
Water purification . . . . . . . . . . . . . . page 3
Laboratory chemicals . . . . . . . . . . . page 3
Spectrophotometry . . . . . . . . . . . . . page 4
Nucleic acid purification . . . . . . . . . page 5
Nucleofection™ . . . . . . . . . . . . . . . . . page 6
Genome editing . . . . . . . . . . . . . . . . page 7
Mycoplasma detection . . . . . . . . . page 8
ChIP . . . . . . . . . . . . . . . . . . . . . . . . . . . . page 9
Epigenetics . . . . . . . . . . . . . . . . . . . . page 9
Augmented microscopy . . . . . . . page 10
Heat-On™ Block System . . . . . . . page 11
Plate washer . . . . . . . . . . . . . . . . . page 12
Microplate readers . . . . . . . . . . . . page 12
NanoDrop™ One . . . . . . . . . . . . . . page 13
Laboratory evaporation . . . . . . . . page 14
Media preparation . . . . . . . . . . . . page 15
Labeling and detection . . . . . . . . page 16
BioNordikaBergman AS • tel 23 03 58 00 • [email protected] • www.bionordikabergman.no
2
• The picture on the cover is copyright ELGA Veolia
Purified water for Clinical Analyzers
Clinical analyzers need to receive a constant and reliable supply of compliant water to produce and reproduce diagnostic tests
accurately. Water quality below accepted standards affects the accuracy of the results, and significantly impacts running costs.
Our MEDICA range delivers consistent water quality and can feed a single clinical analyzer, large automated analyzers and multiple
analyzers on a distribution loop.
The MEDICA water purification systems all feature:
• Flow rates from 7 liters up to 200 liters an hour (1 to 52 US Gallons
an hour)
• Meets Clinical Laboratory Reagent Water (CLRW), (formally CLSI
Type I) standard
• Bypass loop for continual supply of compliant water in emergency
situations to the analyzer
• Service support for maximum operational time
• Low consumable and running costs
© ELGA Veolia
Our ELGA team can work together with project managers, installation
engineers, facility managers and clinical scientists to provide help and
expertise at all stages from concept and design through to the build and
installation.
Which MEDICA water purification system should you use?
It is very important to use the correct water quality which meets both the application and analyzer usage requirements.
Contact us and we will help you find the system best suited for your needs.
Do you have questions about water purification?
Contact Monica Laukas at 404 40 960
[email protected]
Laboratory Chemicals
PanReac AppliChem with almost 75 years experience in the manufacturing of chemical products provides their
customers the widest range and highest quality of laboratory reagents. They are specialists in the manufacture of
inorganic salts, high purity acids and solvents and other products for special applications, guaranteeing compliance
with our customers’ specifications. Their catalogue includes more than 3500 products for laboratory chemical use as for
example:
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Analytical reagents according to ACS, ISO, Ph. Eur. and or USP
High purity solvents for spectroscopy, HPLC and GC
Ultra pure acids for trace metal analysis
Standard volumetric solutions
pH buffers
Reagents and solvents for organic synthesis
Standards for IC, ICP and AAS
Reagents for specific applications as Karl Fischer analysis or Kjeldahl analysis
Reagents for clinical diagnosis
Auxiliary products as detergents and pH indicator papers
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Spectrophotometry
UV-1800 Double Beam Spectrophotometer
• Wavelength range: 190-1100 nm
• Spectral bandwidth: 1 nm
• Stray light; 0,02% (220 nm, Nal)
Campaign Price: Kr. 56 000,-
UV-2600 Spectrometer
Variable spectral bandwidth
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Wavelength range 185-900 nm
Option 220-1400 nm
Variable Spectral bandwidth: 01nm to 5 nm
Stray light 0,005% (220 nm, Nal)
Campaign Price: Kr. 89 950,-
UV-1280 Spectrometer
Our smallest UV-VIS single beam spectrophotometer
• Wavelength range 190-1100 nm
• Spectral bandwidth 5 nm
• Stray light <0,05% (220 nm, Nal)
Campaign Price: Kr. 35 500,-
UV-2700 Spectrometer
Variable spectral bandwidth and very low stray light
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Double monochromator
Wavelength range 185-900 nm
Variable bandwidth 0,1 til 5 nm
Stray light: 0,00005% (220 nm, Nal)
Campaign Price: Kr. 104 000,-
Do you want to learn more about Spectrometry?
Contact Hanne Ramstad at 414 79 591
[email protected]
4
© All pictures on this page are copyright Shimadzu
and gel purification needs
New! Monarch nucleic acid purification kits!
Monarch DNA Cleanup Kits rapidly and reliably purify up to 5 µg of
concentrated, high-quality DNA. These kits utilize a bind/wash/elute workflow
with minimal incubation and spin times. The columns provided with each kit
ensure zero buffer retention and no carryover of contaminants, enabling elution
of sample in volumes as low as 6 μl. Monarch Buffers have been optimized, and
do not require monitoring of pH. Eluted DNA is ready for use in restriction
digests, DNA sequencing, ligation and other enzymatic manipulations.
advantages
• Elute in as little as 6 µl
Monarch Plasmid Miniprep Kit
Advantages:
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Elute in low volumes
Prevent buffer retention and salt carry over with optimized column design
Reduce hands on time with faster protocols and less spin time
Monitor completion of certain steps using colored buffer system
No need to add RNaseThebefore
Monarch starting
DNA Gel Extraction Kit can be used to quickly purify DNA from
Easily label columns using
and other
frosted
surfaces
agarose tab
gels. Unlike
kits, there
is no need to add isopropanol to the melted
Monarch DNA Gel
Extraction Kit
• Prevent buffer retention and salt carryover with optimized
column design
• Save time with fast, user-friendly protocols
specifications
• Binding Capacity: up to 5 µg
• DNA Size Range: ~50 bp to 25 kb
• Typical Recovery: DNA (50 bp to 10 kb): 70–90%
DNA (11–25 kb): 50–70%
• Elution Volume: ≥ 6 µl
• Purity: A260/280 ≥ 1.8
agarose prior to loading on the column, saving you a step.
• Protocol Time:
Gel Extraction: 10 min of spin and incubation time.
PCR & DNA Cleanup: 5 min of spin and incubation time
Specifications:
Monarch PCR & DNA
• Culture Volume: 1–5 ml
• Binding Capacity: up to
20 μg
Cleanup
Kit (5 µg)
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• Compatible Downstream Applications: ligation, restriction
digestion, labeling and other enzymatic manipulations, library
construction and DNA sequencing
Plasmid Size: up to 25 kb
The Monarch PCR & DNA Cleanup Kit (5 µg) can be used to purify DNA from
Typical Recovery: up to
20 μg. Yield depends on plasmid copy number, host strain, culture volume, and growth conditions
a variety of enzymatic reactions, such as PCR, restriction digestion, ligation
Elution Volume: ≥ 30 and
μl reverse transcription. The DNA Wash Buffer provided ensures enzymes,
primers (≤ 25
Purity: A260/280 andshort
A260/230
≥ nt),
1.8detergents and other low-molecular weight reaction
components (e.g., nucleotides, DMSO, betaine) are removed.
Protocol Time: 10½ minutes of spin and incubation time
Compatible Downstream Applications: restriction digestion and other enzymatic manipulations, transformation, transfection
of robust cells, DNA sequencing, PCR, labeling, cell-free protein synthesis, etc.
The improved design of the columns supplied with the Monarch
Gel Extraction and PCR & DNA Cleanup Kits enables elution in
as little as 6 µl, and eliminates buffer retention
Optimized column
The improved design of
the columns supplied
with the Monarch
Gel Extraction and PCR
& DNA Cleanup Kits
enables elution in
as little as 6 μl, and
eliminates buffer
retention.
This is a great, easy-to-use, small
footprint kit... it was great to elute in
such a small volume while feeling confident
Monarch
DNA Gel Extraction Kit
that the elution buffer managed to get to all
&
DNA Cleanup Kit
of Monarch
the surface areaPCR
of the&membrane.
– Michelle, Principal Investigator, Central Michigan University,
Mount Pleasant, Michigan
Made with less
plastic for reduced
environmental impact
Unique, tapered design
eliminates buffer
carryover & allows for
elution in as little as 6 μl
Column tip is
compatible with
vacuum manifolds
Product
Cat.no.
Size
Monarch PCR & DNA Cleanup Kit (5 μg)
T1030S/L
50/250 preps
Monarch Plasmid Miniprep Kit
T1010S/L
50/250 preps
Monarch DNA Cleanup Columns (5 μg)
T1034L
100 columns
Monarch Plasmid Miniprep Columns
T1017L
100 columns
Monarch DNA Cleanup Binding Buffer
T1031L
235 ml
Monarch DNA Wash Buffer
T1032L
25 ml
Advantages:
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Elute in as little as 6 μl
Prevent buffer retention and salt carry over
with optimized column design
Save time with fast, user-friendly protocols
Specifications:
• Binding Capacity: up to 5 μg
Visit NEBMonarch.com
to request a•sample.
DNA Size Range: ~50 bp to 25 kb
Monarch DNA Gel Extraction Kit
T1020S/L 50/250 preps
Columns available separately
Buffers available separately
Monarch DNA Elution Buffer
T1016L
25 ml
Monarch Gel Dissolving Buffer
T1021L
235 ml
Monarch Plasmid Lysis Buffer (B2)
T1012L
2 x 27 ml
Monarch Plasmid Neutralization Buffer (B3) T1013L
110 ml
Monarch Plasmid Resuspension Buffer (B1) T1011L
55 ml
Monarch Plasmid Wash Buffer 1
T1014L
2 x 27 ml
Monarch Plasmid Wash Buffer 2
T1015L
30 ml
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Typical Recovery:
DNA (50 bp to 10 kb): 70–90%
DNA (11–25 kb): 50–70%
Elution Volume: ≥ 6 μl
Purity: A260/280 ≥ 1.8
Protocol Time: Gel Extraction: 10 min of spin
and incubation time. PCR & DNA Cleanup:
5 min of spin and incubation time
Compatible Downstream Applications: ligation,
restriction digestion, labeling and other
enzymatic manipulations, library
construction and DNA sequencing
Questions about Monarch Nucleic Acid Purification?
Contact Gitika Emberland at 940 07 207
[email protected]
© All pictures on this page are copyright NEB
5
Nucleofector™ Devices
Nucleofection™
A Revolution in Electroporation
Electroporation is a transfection technology based on the momentary
creation of small pores in cell membranes by applying an electrical
pulse. Lonza’s Nucleofector™ Technology is an improved electroporation
technology that can help you achieve transfection results never before
possible in primary cells, stem cells and hard to transfect cell lines
with standard electroporation. In contrast to classical electroporation,
with Nucleofection high efficiencies can be reached using much lower
substrate amounts and with only moderate impact on viability. The
comprehensive way in which Nucleofector™ Programs and cell typespecific solutions are developed enables nucleic acid substrates
delivery not only to the cytoplasm, but also through the nuclear
membrane and into the nucleus. This allows for high efficiencies up
to 99% and makes the transfection success independent from any cell
proliferation.
4D-Nucleofector™ system
1-16 samples per run
Cell range per sample from 104 to 107
Y-unit for transfection of adherent cells
96 wells Shuttle™ system
Up to 96 samples per run
Cell range per sample from 104 to 106
The Nucleofector™ Technology Relies on a Unique
Combination of:
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A Nucleofector™ Device: Delivering optimized electroporation
parameters pre-programmed for each optimized cell type
Nucleofector™ Kits for primary cells or cell lines: Containing a
specific Nucleofector™ Solution that provides a protective
environment that allows for high transfection efficiency and cell
viability, while helping to maintain physiologically relevant
cellular functions
Cell-specific Optimized Protocols: Providing optimal Nucleofection™
Conditions and comprehensive guidance, including tips for cell
sourcing, passage, growth conditions and media, and
post-transfection culture
Benefits over Standard Electroporation at a Glance
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Electroporation parameters are optimized to gain high transfection
efficiency and retain highest viability
Excellent preservation of the physiological status of transfected cells
More than 650 cell-type specific protocols for direct transfection
success with a multitude of different cell types
Highly-skilled scientific support team to assist you in your research
Invention of Nucleofection of cells in adherence
Genome Editing - Successful Transfection of CRISPR & Co.
using Nucleofector™ Technology
HT Nucleofector™ system
Up to 384 samples per run
Cell range per sample from 104 to 106
Nucleofector™ 2b system
1 sample per run
Cell range per sample from 105 to 106
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© All pictures on this page are copyright Lonza
All genome editing tools require co-transfer of several substrates into
the cell type of interest for successful modification of genomic DNA.
Lonza’s non-viral Nucleofector™ Technology has been shown to work
as a reliable and sufficient method for transferring the required DNAor RNA-based components into various cell lines and primary or stem
cells, e.g., primary T cells, human embryonic stem cells (hESC) or
induced pluripotent stem cells (iPSCs).
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High transfection efficiencies for a broad range of cell types,
including iPSCs
Efficient co-transfection of various substrates
Same conditions for transfecting plasmids, DNA, mRNA or PCR
cassettes, ssODN
Proven for ZFN, TALEN and CRISPR by more than 30 publications,
including high ranking journals
Do you want to learn more about Nucleofection?
Contact Kristin Lundemo at 992 27 101
[email protected]
NEB offers Cas9 NLS
and other tools that support
Genome Editing Workflows.
Visit international.neb.com/genomeediting to learn
more about the basics of CRISPR/Cas and related applications
7
Mycoplasma detection
Mycoplasma Contamination in Cell Cultures
One of the most common contaminants present in cell
culture laboratories are mycoplasma1. A conservative
estimate states that between 15-35% of all continuous
cell cultures are contaminated with mycoplasma, some
estimates are even higher (up to 80% in some countries2).
Mycoplasma Effects on Your Cell Culture
Typical routes of infection are cross-contamination from
untested infected cells (e.g. via aerosols generated during
pipetting, use of same media bottles, handling of more
than one cell type at one time), contaminated materials,
contaminated donor tissue (<1%) or direct infection from the
researcher. The primary source is normally cross-contamination from infected cultures. Mycoplasma grow slowly and
do not kill the cells outright but affect various cellular parameters 1,3,4,5,6,7,8,9,10,11. Thus, mycoplasma contaminations
can seriously impact the reliability, reproducibility, and
consistency of experimental results, representing a major
problem for basic research as well as for the manufacturing
of bioproducts. Standard testing for mycoplasma is an
important quality control.
Detect, Eliminate and Prevent Mycoplasma
Contamination
Lonza provides a powerful product offering for reliable
detection and successful elimination and prevention of
mycoplasma contaminations:
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Detection:
Easily detect mycoplasma using the MycoAlert™ PLUS
Mycoplasma Detection Kit with your own luminometer
or Lonza’s single-tube Lucetta™ Luminometer tailormade with a MycoAlert™ Protocol wizard
Elimination:
Efficiently eliminate mycoplasma with the MycoZap™
Elimination Reagent
Prevention:
Reliably prevent mycoplasma with MycoZap™
Antibiotics or MycoZap™ Spray
Product
Cat.no.
Size
MycoAlert™ PLUS detection kit
LT07-701
10 tests
MycoAlert™ PLUS detection kit
LT07-703
30 tests
MycoAlert™ PLUS detection kit
LT07-705
50 tests
MycoAlert™ PLUS detection kit
LT07-710
100 tests
MycoAlert™ Assay Control Set
LT07-518
10 tests
MycoAlert™ PLUS detection kits
Lucetta™ Luminometer
Lucetta™ Luminometer
AAL-1001
MycoZap
MycoZap™ 1 treatment kit
LT07-818
1 treatment
MycoZap™ 5 treatment kit
LT07-918
5 treatments
MycoZap™ Plus-CL
VZA-2011 10 x 1 ml
MycoZap™ Plus-CL
VZA-2012 1 x 20 ml
MycoZap™ Plus-PR
VZA-2021 10 x 1 ml
MycoZap™ Plus-PR
VZA-2022 1 x 20 ml
MycoZap™ Prophylactic
VZA-2031 10 x 1 ml
MycoZap™ Prophylactic
VZA-2032 1 x 20 ml
MycoZap™ Spray
VZA-2001 500 ml
MycoZap™ Spray, Refill
VZA-2002 1000 ml
Mycoplasma References
1) Drexler HG, Uphof CC (2002). Cytotechnology 39: 75–90
2) Koshimizu K, Kotani H (1981). In: Procedures for the Isolation and Identification of Human, Animal and Plant Mycoplasmas (Nakamura, M., ed.),
Saikon, Tokyo, 87-102
3) Gong H et al. (1999). Biochem Biophys Res Comm 261: 10-14
4) Ben-Menachem G et al. (2001). FEMS Microbiol Letters 201: 157-162
5) McGarrity MF et al. (1984). In Vitro 20: 1-18
6) Sokolova IA et al. (1998). Immunol Cell Biol 76: 526-534
7) Doersen CJ, Stanbridge EJ (1981). Mol Cell Biol 1: 321-329
8) Stanbridge EJ (1971). Bacteriological Reviews 35): 206-227
9) Darin N et al. (2003). BMC Biochem 4:15
10) Rottem S (2003). Physiol Rev 83: 417-432
Do you want to learn more about mycoplasma detection?
11) Miller C et al.(2003). Biotechniques 35:812-814
Contact Kristin Lundemo at 992 27 101
[email protected]
8
© All pictures on this page are copyright Lonza
SimpleChIP® Enzymatic ChIP Kits
from Cell Signaling Technology
SimpleChIP® Enzymatic Chromatin IP Kits from Cell
Signaling Technology are optimized to detect endogenous levels of protein-DNA interactions in both
cultured cells and SimpleChIP® Plus Chromatin IP Kits
offer the additional benefit of tissue sample analysis.
These kits contain all reagents necessary to perform
enzymatic
digestion-based
chromatin
immunoprecipitation (ChIP) experiments quickly and easily, as well
as positive and negative controls that ensure confidence
in your results. These kits are available with either
Protein G agarose or Protein G magnetic beads and
contain all buffers and reagents needed to perform up to
30 ChIP assays.
© Cell Signaling Technology
Benefits include:
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Enzymatic digestion of chromatin is milder than
sonication and better preserves the integrity of the
chromatin and antibody epitopes, which means
increased IP efficiency
• A detailed and easily scalable protocol optimized for
analysis of tissue and cell samples is provided, saving
your valuable time and reagents
• All reagents and controls are included, saving valuable
time and providing the proper experimental controls
• These kits are used in-house for validation of our ChIP recommended antibodies, saving you the trouble of
optimization
• The included ChIP-grade magnetic beads are ideal for
downstream ChIP-on-chip or ChIP-sequencing as they
provide higher sensitivity and low background
• Technical support is provided by the same scientists
who developed and produce the kits, ensuring a
thorough, fast, and accurate response
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Augmented Microscopy™
capture
analyze
Cellular compartments are automatically
segmented and analyzed. Annotation
tools are available.
annotate
video
Includes temperature, gas, humidity
control and a movie maker for live cell
time-lapse imaging.
Lionheart™ FX Automated Live Cell Imager enables superior digital microscopy with high resolution images
up to 100x. From simple fixed cell assays and slide scanning to advanced, environmentally controlled timelapse movies and 3D spheroid formation imaging, Lionheart FX
and Gen5 3.0 Software provide qualitative and quantitative data
in a compact automated microscopy system.
Visit www.lionheartfx.com
www.biotek.com
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Heat-On™ Block System
The safer alternative to oil baths
Heat-On accepts vials, tubes and flasks from 10ml to 5 litres.
Key Benefits:
•Replace messy oil baths, heating mantles and avoid spills
• Make your chemistry safer, cleaner and faster
• Solid aluminium blocks provide even heating
• Lightweight design allows rapid heating
• Unique well design eliminates cracking of flasks
• Blocks feature two probe holes for added convenience
• Optional and detachable lifting handles
• Optional PTFE Safety Covers reduce risk of user touching hot blocks
• Block for Florentine (pear-shaped) flasks
• Use up to 260˚C
• Fit on all leading hotplate brands with a 145mm Ø top plate; optional adapter plate for 135mm Ø top plates
Choice of block finishes:
• Fluoropolymer coating gives superb chemical resistance
• Anodised finish is durable, lower cost option
Oli baths are messy, dangerous and costly:
• Oil will discolour and degrade with repeated heating and contamination from chemicals, water and general debris
• Degradation results in a dangerous lowering of the oil’s flashpoint as well as increased smoking and fumes
• Oil should be regularly replaced to maintain a safe working environment
• Disposal of oil waste must be done in an environmentally and safe way, with associated costs
Special Offer!
Buy 2 Heat-On™ products and get a 3rd free!
Reasons for choosing Heat-On:
Cleaner, greener, safer!
The risk of oil fires and injury from hot oil spills, plus the mess
and cost associated with the use of oil means that oil baths no
longer represent safe working practice in labs. Heating mantles are expensive, difficult to clean, do not respond well to
spills and often create hot spots when heating.
Scientists are increasingly turning to specially designed aluminium blocks located on stirring hotplates to heat standard round
bottom flasks.
© Radleys
Do you want to learn more about the products from Radleys?
Contact Marte Tøften at 922 11 099
[email protected]
11
ELx50 strip washer
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Washes 96- and 384-well microplates
Magnetic and polystyrene bead assays
ELISAs and cell-based assays
Filtration-to-waste processes
Patented Dual-Action™ wash manifold for independent control of dispense and aspiration tubes
Comprehensive onboard software makes programming quick
and easy
Automatic switching for up to three wash buffers
Programmable shaking duration and intensity
Built-in maintenance programs
Epoch & Take3 nanodrop plate
A microplate reader and a nanodrop in one instrument
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Save valuable sample by using Take3 to quantitate
2 μL volumes of DNA, RNA and proteins
Monitor cell growth using the Epoch’s well scanning capability
Run spectral scans of 2 μL samples, or samples
contained in a microplate or cuvette
Dial in individual wavelengths from 200 – 999 nm
Perform rapid qualitative or quantitative analysis in microplates
from 6- to 384-wells
Measure kinetic rates of reaction
Run multiplex assays quickly and easily
Save space on the benchtop with its very small footprint
Avoid costly maintenance with the robust, thoughtfully
engineered optics and hardware
Never purchase costly absorbance filters
MultiFlo FX Dispenser/Washer
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Up to four independent reagents dispensed in parallel with no potential for carryover
Unique Parallel Dispense technologies – choice of
peristaltic or syringe pumps for unique assay requirements
Large, high resolution color touch screen display
Onboard computer with two USB flash drive ports for file transfer, storage and operation
Optional plate washing module increases functionality
Modular and upgradable
ELISAs, cell-based assays (including automated staining and fixing for imaging) and bead-based assays such as Luminex
xMAP®
Do you want to learn more?
Contact Hanne Ramstad at 414 79 591
[email protected]
12
© All pictures on this page are copyright BioTek
MOLECUL AR SPECTROSCOPY
Product Specif ications
Thermo Scientific
NanoDrop One Microvolume
UV-Vis Spectrophotometers
Intelligent microvolume analysis
Thermo Scientific™ NanoDrop™ One microvolume
UV-Vis spectrophotometers quantify and qualify
DNA, RNA, and proteins in seconds using only
1–2 µL of sample.
These next-generation NanoDrop instruments are built
with Thermo Scientific™ Acclaro™ Sample Intelligence
technology that helps you understand the quality of
your sample before using it in downstream applications,
bringing you one step closer to discovery. With
contaminant analysis, sample information alerts and
on-demand technical support, the Acclaro technology
brings a new level of confidence in results, making
NanoDrop One the ideal UV-Vis spectrophotometer
for life science researchers.
Easily transfer data from NanoDrop One
standalone instrument to your computer
via a USB device.
Pipette. Measure. Know.
Expect NanoDrop reliability and enhanced
performance with these NanoDrop One
advantages:
Fast and easy evaluation of nucleic acids
and proteins with Auto-Measure and
pre-programmed applications
Modern standalone design with local
control and high-resolution touchscreen
interface saves bench space
Accurate measurements up to
27,500 ng/μL (dsDNA) with extended
auto-range pathlength technology
No consumables needed– pipette directly
onto the pedestal sample-retention system
Enhanced connectivity with data
transfer via USB, Ethernet, Bluetooth® and
Wi-Fi options; PC software available for data
management
Optional cuvette position for measuring
dilute solutions and performing temperature
sensitive experiments
Know More with Acclaro Sample
Intelligence Technology
Make informed decisions on sample suitability
for your application. Acclaro technology
offers enhanced sample analysis with:
• Contaminant identification and corrected
results
• Instant feedback about sample quality with
on-demand technical support and guided
troubleshooting
• Embedded sensor and digital image analysis
that ensures measurement integrity
Add a Cuvette Position
The Thermo Scientific™ NanoDrop™ OneC
spectrophotometer adds
experimental flexibility and
increases the dynamic range.
Use cuvettes to measure
dilute samples and optical
density of bacterial cultures or to
perform kinetics experiments. Includes
cuvette temperature control and stirring.
Do you want to learn more about NanoDrop One?
Contact Kristin Lundemo at 992 27 101
[email protected]
13
Laboratory Evaporation
Rotavapor® R-300
Convenient and efficient rotary evaporation
The Rotavapor® R-300 represents high reliability and
convenience in laboratory rotary evaporation applications. The Rotavapor® R-300 is available with either
an electronic or manual lift, and can optionally include
the Heating Bath B-301 or B-305 and, a glass assembly
of your choice and the Interface I-300 or I-300 Pro. All
accessories and connections are included for out of the
box usage.
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Comfortable flask and vapor duct removal thanks
to easy to use Combi-Clip
User-friendly operation due to ergonomic electric
or manual lift
Immediate adjustment of heating temperature
and rotation speed with intuitively positioned
control knobs
Easy plug & play extension to a fully integrated
system including a central interface, vacuum pump
and recirculating chiller according to your needs
Choice between 5 L high-temperature and 1 L fast-heating bath
Wide range of beneficial accessories available: seven different glass assemblies, safety shield, lid for heating bath, automatic
distillation and automatic foam detection
Precise operation control with integrated digital display of heating temperature, rotation speed and lift position
Traceability of all process parameters including data recording and charting with the optional Interface I-300 Pro
Instant alert with push notification on mobile devices in case of process deviation (with Interface I-300/I-300 Pro only)
Reference:
“The method-based guided operation simplifies the distillation task. Thus, we can entrust the lab assistants to safely perform a reproducible
purification process using the Rotavapor® system, and dedicate ourselves to other tasks.” Dr. Yasuhiro Shirai, Group Leader Photovoltaic Polymer
Materials Global Research Center for Environment and Energy based on Nanomaterials Science, GREEN, Japan.
Rotavapor® R-100
Economical evaporation
The Rotavapor® R-100 is a high-quality rotary evaporator to meet the essential
needs in clas¬sical laboratory applications. It is capable of a manually adjustable
rotation speed of 20 to 280 rpm and equipped with an action jack lift for manual
lifting of the evaporating flask. Included is the selected condenser, 1 L receiving
and evaporating flasks and a vapor duct. All accessories are included for out of
the box usage.
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Effective Rotavapor for limited budgets
Fundamental design fulfills essential needs of evaporation
Quick and ergonomic operation with manual lift mechanism
Easy flask handling and vapor duct removal with Combi-Clip
Easy operation by integrating interface, vacuum pump and
recirculating chiller
Expandable with a wide range of accessories and various glass assemblies
Flexibility to use the independent heating bath for further applications
Do you want to learn more about our rotavapors from Buchi?
Contact Marte Tøften at 922 11 099
[email protected]
14
© All pictures on this page are copyright Buchi
Media preparation
MEDIAJET
The MEDIAJET system offers the unique flexibility to fill Petri
dishes of various sizes, Petri dishes with two compartments as
well as test tubes of various diameters and length. At the same time,
MEDIAJET requires only minimal bench space in the laboratory.
• Easy handling: The intuitive, full size graphical user interface makes it very easy to control
all functions of MEDIAJET. The operation of the system is entirely self-explanatory, as all
functions and prompts are explained in plaintext.
•
Reliable walk-away automation: With the novel Feed-In / Stack-Out technology, MEDIAJET guarantees
a reliable and completely user independent operation. Typical production variations in the diameter or shape of the Petri
dishes are easily handled by the unit, as they are actively guided throughout the entire filling process. Moreover, the optical dish
sensors of MEDIAJET can be easily adjusted to virtually every Petridish brand at the user interface level.
• Media cost reduction: MEDIAJET has a built-in “Agar Spread Function” which ensures a homogenous distribution and an even
surface of the agar. It helps to optimize the agar level in the Petri dish and thereby allows a significant reduction of media costs.
•
Eliminating sources of contamination: For consistent agar plate quality, a clean environment during the dispensing process is
essential. The surface of the filling chamber is manufactured of a single piece of resistant PE, which allows convenient and
efficient cleaning. In addition, MEDIAJET is equipped with a UV lamp extending over the full length of the rotor where the dishes
are opened during the dispensing process. The lamp emits powerful 2.1 W UV-C radiation for optimal bactericidal efficiency in
the area most vulnerable to contamination.
• Biplate: For many applications, the amount of required agar can be reduced by using two-compartment Petri dishes. However,
pouring biplates by hand is time-consuming and inefficient. With the MEDIAJET vario biplate option you have an efficient and
reliable solution to fill two-compartment Petri dishes automatically.
Dose IT peristatic pump
Store Application Protocols. Up to 20 application
protocols can be stored and recalled anytime.
Compact Design
This pump fits everywhere and can be moved easily.
Large Display
Allows for an intuitive programming and operation.
Fliptop Pumphead
Allows for an easy and quick tubing exchange
Different tubing sizes. Compatible with tubing from
1 to 8 mm ID, DOSE IT allows fast and precise
dispensing from 100 µl to several litres.
Do you have questions about the products from Integra?
Contact Monica Laukas at 404 40 960
[email protected]
© All pictures on this page are copyright Integra
15
PIONEERS in State of the Art
Labeling & Detection
In Situ Hybridization
DNA/RNA Applications
Immunofluorescence
Immunohistochemistry
Multiple Antigen Labeling
Neuronal Tracing
Blot Detection
Enzyme Immunoassays
Glycobiology Studies
Cellular Imaging
Since
1976
As a U.S. based primary manufacturer of the highest quality detection
reagents, Vector Laboratories is able to provide our products to
customers in standard, bulk and OEM formats, at low cost directly
from our facilities.
Producers of innovative products including: VECTASTAIN® ABC Kits • ImmPRESS™
Enzyme Polymer Reagents • Mouse on Mouse (M.O.M.™) Kits • ImmPACT™
Substrates Avidin/Biotin Systems • Secondary Antibodies • NEUROBIOTIN™
Tracers • Lectins VECTASHIELD® Mounting Media
w w w. v e c t o r l a b s . c o m
BioNordikaBergman AS
•
tel 23 03 58 00
bergman
•
fax 23 03 58 01
•
[email protected]
•
Promo code: NL-2/16
www.bionordikabergman.no
Note! The promo code is required to obtain the special prices!
The offers are valid until 31.08.16 and cannot be combined with other
offers or agreements. The prices are listed excl. VAT and shipping charges.
We reserve the right to typos.
BioNordikaBergman AS • tel 23 03 58 00 • [email protected] • www.bionordikabergman.no