BIO-WEST Riffle Beetle Habitat Connectivity Study
Transcription
BIO-WEST Riffle Beetle Habitat Connectivity Study
BIO-WEST Riffle Beetle Habitat Connectivity Study PROPOSAL - # 132-14-HCP Comal Springs Riffle Beetle Habitat Connectivity Study 1 BIO-WEST Riffle Beetle Habitat Connectivity Study Table of Contents EXECUTIVE SUMMARY ..........................................................................................................3 INTRODUCTION ..........................................................................................................................5 POTENTIAL PACKAGES ................................................................................................................10 DEGREE OF COMPLIANCE ...........................................................................................................10 PROPOSAL PRICING (Under separate cover) ................................................................................10 Attachment A1....................................................................................................................................11 Attachment A3 ...................................................................................................................................13 SCOPE OF WORK ............................................................................................................................................ 15 PROPOSED SCOPE ALTERATIONS ............................................................................................................... 21 QUALIFICATIONS ....................................................................................................................22 PROJECT TEAM ...................................................................................................................................................... 22 KEY PERSONNEL .................................................................................................................................................. 23 RELEVANT EXPERIENCE .................................................................................................................................. 26 WORK SCHEDULE ...................................................................................................................38 REFERENCES ..............................................................................................................................38 Attachment B1 ............................................................................................................................................................ 39 Attachment B2 ............................................................................................................................................................ 40 Attachment B3 ............................................................................................................................................................ 41 RESUMES .......................................................................................................................................42 2 BIO-WEST Riffle Beetle Habitat Connectivity Study EXECUTIVE SUMMARY As is evident in the attached proposal, BIO-WEST, Inc. has assembled a project team with extensive experience and technical expertise in conducting applied research with the endangered species and their habitats of the Comal and San Marcos Springs/River ecosystems. To assist with this project, BIO-WEST has enlisted the assistance of Dr. Weston Nowlin of Texas State University, Dr. Robert Doyle of Baylor University, and the invertebrate specialists at Zara Environmental. Factors that set the BIO-WEST study team apart from the competition include: BIO-WEST scientists and collaborators have been conducting continuous monitoring and Applied Research related to the Comal and San Marcos endangered species and springs habitat for over 14 years. o We are currently conducting four separate EARIP HCP Applied Research efforts in 2014 (Fountain darter Movement, Fecundity, and Predation as well as a low-flow response study for the Comal Springs riffle beetle) that was conducted after the completion of four separate Comal Springs riffle beetle studies in 2013 (Field vs. Lab, Vegetation Tolerance, Food Source, and pH drift). All eight have been designed based on the intent of the HCP; have been presented and approved by the current HCP science team; will be completed on time and on budget; and will lead to direct use for parameterization in the HCP Ecological model or directly address critical HCP Phase II questions. o Additionally, Dr. Nowlin is conducting the Comal Spring riffle beetle plastron study as part of 2014 HCP Applied Research funding. o BIO-WEST has also conducted additional Applied Research efforts through the EAA Variable Flow Study that were directly related to endangered species (fountain darter, Comal Spring riffle beetle, and Texas wild-rice). These efforts have been extremely valuable in shaping the HCP and informing the need for this additional HCP related research. BIO-WEST thoroughly understands the Intent of the HCP as well as the long-term biological goals and underlying flow management regime. Many assumptions were made regarding the development of the flow regimes for the Comal and San Marcos rivers that were incorporated into the final HCP. The uncertainty embedded in such assumptions adds to the importance of the applied research and restoration activities proposed during Phase I of the HCP. This understanding is critical to the design of applied research efforts specific to the HCP so that it can be used to assist with the tough Phase II decisions that are rapidly approaching. BIO-WEST is the prime contractor on the HCP Ecological Modeling team. As such, BIOWEST thoroughly understands the direction and complexities of that critical modeling effort and is well positioned to design studies that will be directly useful to that modeling effort. Applied research efforts that are not designed with the end goal being incorporation into the Ecological Model or directly addressing Phase II decisions will likely end up being interesting academic exercises with little EARIP HCP utility. 3 BIO-WEST Riffle Beetle Habitat Connectivity Study On a personal level: Mr. Ed Oborny (BIO-WEST) has spent the majority of his career working with aquatic plants and animals of the Comal and San Marcos ecosystems. He understands these systems but more importantly, truly enjoys sharing this information with collaborating scientists, Board of Directors, area school children and teachers, science teams, etc… Plus, he has a track record of well over a decade for the Authority of getting projects done on time and on budget. Mr. Randy Gibson (Senior Macroinvertebrate Biologist at BIO-WEST) is the foremost expert on the Comal Springs riffle beetle and has been conducting bio-monitoring and experimentation through BIO-WEST on this species for well over a decade. Dr. Weston Nowlin (Associate Professor – Texas State University) is an expert at assessing how biotic and abiotic factors affect the abundance, distribution, and diversity of organisms in ecosystems. Dr. Robert Doyle (Director of the Center for Reservoir and Aquatic Systems Research at Baylor University) is the foremost expert with aquatic vegetation specific to the Comal and San Marcos rivers. Dr. Doyle has also be Co-Principal investigator on three of the eight applied research studies conducted by the BIO-WEST team to date and served in a QA/QC role on the remaining five. Unparalleled scientific integrity. Dr. Doyle, Mr. Oborny and Dr. Jean Krecja (Zara) are active members of the Southern Edwards Aquifer Species Recovery Team. Finally, the BIO-WEST project team has all the necessary federal and state endangered species permits to begin this work immediately upon award. We are pleased to submit this proposal to the Authority for consideration. It will become evident as you explore this proposal that project team personnel have extensive experience and expertise in applied research and the ecology of the Comal and San Marcos river systems. Examples of relevant experience are included along with a scope of work for 2015 activities. Information on key team members is included and costs are detailed by task under separate cover. 4 BIO-WEST Riffle Beetle Habitat Connectivity Study INTRODUCTION Section 6.3.4 of the Habitat Conservation Plan lays out the path forward for answering key questions and filling in data gaps to test Phase I assumptions and ultimately assist with Phase II decisions. The focus in 2013 was on addressing several key questions surrounding physical habitat and food source responses, both related to the fountain darter. In 2014, additional Tier A and Tier B applied research was conducted focused on the fountain darter and Comal Springs riffle beetle. A wealth of valuable information has been acquired over the first two years and the third round (2015) of applied research targets specific inputs to the HCP ecological model and filling critical data gaps in supporting Phase II decisions. Being that eight individual applied research efforts are being proposed for 2015, we feel it is important upfront that the reviewers understand our thought process for how we selected the studies for proposal and how we determined the level of work and budget (under separate cover) needed to address the key questions proposed in the RFPs. Our assessment of the priority of the proposed 2015 Applied Research efforts was based on their ability to 1) directly answer key questions critical to support Phase II decisions and 2) support inputs and/or testing of the HCP ecological model that will in turn assist in answering Phase II questions. In addition, we also felt a third key factor was whether their underlying hypothesis is best controlled by a change in the flow regime and not other management activities. To illustrate our thoughts, we have included Table 1 below. No surface flow this summer at the headwaters of Spring Run 1 of the Comal System. 5 BIO-WEST Riffle Beetle Habitat Connectivity Study TABLE 1 – PROJECT TEAM CRITERIA Study Rank Critical Data Gap for Phase II decision making Use in existing HCP Ecological Model(s) Dependent on Flow regime YES - Aquatic Vegetation response (SAV model) and dissolved oxygen (water quality submodel) YES Algae Dynamics #134-14-HCP 1 Within context of Ecological model Riffle Beetle Connectivity #132-14-HCP 2 CRITICAL to understanding of impacts at Comal System Undetermined - At present, there is not a CSRB model in development YES Ludwigia interference #137-14-HCP 3 Within context of Ecological model YES - Aquatic Vegetation Response (SAV model) YES Texas Wild-rice suspended sediment #133-14-HCP 4 Within context of Ecological model and important relative to potential recreational Impacts YES – Texas wild-rice growth (SAV model) and recreation impacts (impacts assessment) Not necessarily – May be best controlled by restricting recreation Food Source Refinement #130-14-HCP 5 Within context of Ecological model YES – refinement of model inputs for food source YES 6 Important relative to potential recreational Impacts Undetermined at this time Not necessarily – May be best controlled by restricting recreation 7 Important - But likely more applicable for refugia research Undetermined at this time YES Undetermined at this time To be determined during 2015 Modeling activities – best suited for 2016 Applied Research YES Fountain Darter Turbidity #131-14-HCP Fountain Darter Compensatory #135-14-HCP Aquatic Vegetation Shear Stress study #136-14-HCP 8 In our opinion, simply dividing the $450k up equally and having eight $50k studies will only answer a few questions in total and leave the more critical questions incomplete and unanswered. With that said, we are sure that the Authority will receive proposals with a wide range of costs for each of these studies including several low-ball offers from firms who do not fully understand the HCP intent or Phase II decisions that have to be made. We also fear the tendency of some bidders will be to simply propose “academic” exercises. Based on our professional judgment from working in both systems for a long time, what we have learned from 2013 and 2014 HCP sponsored applied research, and our understanding of the HCP intent and ecological modeling activities, we offer the following as our interpretation of how we determined the resources necessary to accomplish the goals for these studies. 6 BIO-WEST Riffle Beetle Habitat Connectivity Study All eight studies are well thought-through and meet the HCP intent to enhance our ecological understanding of system processes. However, we feel studies 1 through 3 below are critically important because of the time frame of the HCP Phase I / Phase II and ecological modeling activities. As such, our first focus was to determine the resources necessary to complete these studies in 2015. A summary of our rationale is as follows: 1) Algae dynamics and dissolved oxygen depletion (#134) – Why? During low-flow conditions bryophytes die off, aquatic macrophytes are coated with algae and start to decay, and, as we witnessed for the first time this past summer - even the algae dies off under extreme conditions. Submerged aquatic vegetation (bryophytes, rooted macrophytes, and even algae) serve as fountain darter habitat and are direct parameters being assessed within the ecological model. Further understanding the algal survival and decay dynamics during extended periods of low flow will greatly enhance the ability to predict survival of the fountain darter in the existing HCP ecological model. In addition, dissolved oxygen (DO) in Landa Lake via 2013 and 2014 summer low-flow monitoring shows periods of DO < 2.0 mg/L. Firing up the aerator system in Landa Lake resulted in less than a 1 mg/L increase in dissolved oxygen. Dissolved oxygen is a critical parameter within the ecological model and understanding the dynamics at low flow will greatly enhance the ability to predict survival of the fountain darter. 2) Riffle Beetle Connectivity (#132) – Why? We know a considerable amount about the riffle beetle distribution and are gaining information on the population size and low-flow effects during 2014. However, very little is known on the surface / subsurface interactions and how that affects the population and/or distribution of beetles. Applied research in 2013 and 2014 demonstrated that aquatic vegetation as fountain darter habitat and fountain darters themselves are quite tolerant suggesting that the Comal Springs riffle beetle may in fact be more of a sentinel species than the fountain darter. The present HCP flow regime exhibits periods of extended drying of the spring runs, western shoreline and spring island areas (these areas are the presumed strong hold for the riffle beetle in the Comal System). Although the HCP flow regime is not projected as severe on the minimum end as experienced in the drought of record (DOR), these projected HCP extended periods of < 100 cfs are well beyond what was observed in the DOR. With the Comal Springs riffle beetle likely shifting to be the sentinel species for the Comal system, understanding their tolerance and surface/subsurface interactions is vital to making Phase II decisions. 3) Ludwigia interference study (#137) – Why? Since the fountain darter is documented as having higher densities in native versus non-native vegetation, the importance of native vegetation restoration is magnified. A key question is how to code native vegetation competition with nonnative vegetation within the HCP ecological model following disturbance events (scour, mitigation projects, recreation, etc.). How this is coded in the ecological model will play a vital role in model output pertaining to the preservation or loss of quality fountain darter habitat in both the Comal and San Marcos systems over time. Providing input data gathered through actual studies versus opinion or literature for other species is mandatory to more accurately portray these aquatic vegetation responses. This study will also assist with assessing the level of aquatic gardening that is required for successful native vegetation restoration. Based on our assessment, it will be evident in our Scopes and budgets (separate cover) that the activities planned and resources requested for 1 and 2 exceed an equal $50k distribution of funding of all eight studies, while # 3 is actually less. However, even with our proposals, considerable monies are still available to embark on additional studies, thus we continue. 7 BIO-WEST Riffle Beetle Habitat Connectivity Study We feel studies 4, 5 and 6 (Table 1 - #133, #130 and #131) provide the next level of importance as they meet the HCP intent for ecological understanding, will serve as potential inputs to the Ecological Model, and are either dependent on the flow regime or address the key aspect of recreation. 4) Texas wild-rice suspended sediment (#133) – Why? Relative to the HCP Ecological model, the ability to understand Texas wild-rice growth is of less importance because of the incredible success of the restoration activities and response to lower flow conditions. There is over 6,000 m2 of Texas wild-rice in the system this year after repeated low-flow events in 2006, 2009, 2011, 2013 and 2014. However, this study is very important relative to examining recreational impacts to the system. With that said, it is not entirely flow-dependent as controlling recreation may in fact be a better way of decreasing these impacts than simply allowing for more flow. 5) Food source refinement (#130) - Why? Understanding the dynamics and thresholds of fountain darter food sources is essential to the development of a more accurate representation of impacts to the fountain darter in the ecological model. Based on the 2013 laboratory and pond food source study results, two additional applied research topics were proposed to further refine ecological model parameters. The first is to evaluate the temperature range between 28 °C and 34 °C to more accurately determine a threshold temperature for amphipods. This is important in that, at 28 °C, fountain darters can exist just fine and reproduce to a limited degree, but near 34 °C their reproduction shuts off and survival becomes tenuous. If food really should become limiting at 29 °C rather than 33.5 °C, there is the potential for this parameter to be extremely important. However, if the threshold is truly more near 34 °C, then direct temperature impacts to fountain darters would likely trump any food source response in the ecological model. The second proposed investigation is to evaluate whether Vallisneria or Ludwigia provide similar benefits to what was experienced with Riccia. The reason for this investigation is that the bryophytes are likely the first plant species to be eliminated from the system during low flows while Vallisneria and Ludwigia should persist. 6) Fountain darter Turbidity (#131) – Why? Turbidity affects light and light affects plants and therefore, it seems turbidity’s effect on fountain darter habitat might be considered the foremost question. That is a significant question but fortunately one that is answered in detail in the literature and currently being incorporated into the submerged aquatic vegetation module of the HCP Ecological model. However, turbidity also has the potential to affect the fountain darter relative to their ability to feed. If turbidity does impact the feeding ability of the darter it is very likely that it might also impact other fish species (i.e. darter predators), thus magnifying the complexity by requiring testing of known predators to firmly answer the applicable HCP Phase II question. This point has been raised by Dr. Tom Arsuffi (HCP Science committee) on several occasions. The complexity surrounding these issues is immense and will require a series of experiments to be conducted in both the laboratory and field setting. This study specifically addresses potential recreational impacts and thus will be important for management decisions. Out of professional respect for the researchers who proposed and designed the Texas wild-rice suspended sediment study, BIO-WEST did not submit a proposal for that project. With that said, had we proposed on that project, our cost estimate would have been close to the equal distribution of $450,000 / 8 studies for intensive field sampling prior to, during, and after the 2015 recreation season as well as following storm events to fully capture the spectrum of potential impacts and tease out causes relative to increased turbidity. We feel studies 5 and 6 (Table 1) could be effectively conducted for slightly over the equal distribution of $450,000 / 8 studies. As such, it is very possible that five or six applied research studies could be funded during 2015 and designed to provide significant contributions to the HCP under the assumption that $450,000 is available for use. 8 BIO-WEST Riffle Beetle Habitat Connectivity Study Should additional funding be available, Table 1 shows the order for how we would rank the fountain darter compensatory reproduction study (#135) and shear stress aquatic vegetation study (#136). In our opinion, the compensatory reproduction study is a fascinating academic exercise but likely too complex for incorporation into the present ecological model without significant resources and follow-on studies. It will likely provide a benefit from a refugia research component and thus will be valuable to the HCP down the road. Although the shear stress study was specifically submitted by the HCP Ecological Modeling team back in the spring, the current status of analysis of the empirical aquatic vegetation scour data in both systems being conducted by the Ecomodel team right now suggests that it may not be necessary to complete that study to parameterize the model. Regardless, in our opinion, it is too early to tell with the analysis and modeling extending into 2015 for that assessment. As such, it is our opinion that should the shear stress study be necessary, it be slated for 2016 applied research. We apologize upfront that we have included this same verbiage in each of our four submittals, but we wanted the Authority to understand our rationale for scopes and budgets. In this particular proposal, we feel the Comal Springs Riffle Beetle Habitat Connectivity Study (#132-14-HCP) is critically important in the context of the HCP Ecological Model, Phase I testing, and Phase II decision making. Initial testing of the Riffle Beetle Aquifer Simulation System (RBASS) at the FAB wet lab. 9 BIO-WEST Riffle Beetle Habitat Connectivity Study POTENTIAL PACKAGES In going through this exercise of prioritizing and scope development, we have identified some potential package options below for the Authority to consider. We feel each has the potential to enhance efficiency and effectiveness of 2015 applied research efforts (basically save time/money and space). Package A • Algal Dynamics and Dissolved Oxygen depletion (#134) Ludwigia interference (#137) Rationale: Both these studies involve teaming with Baylor University for field and laboratory components so costs associated with travel to and from the site and could be reduced from an efficiency standpoint. o Both studies will involve extensive field testing components in order to fully address the questions at hand. As such, close coordination of both field efforts would save additional time and effort. o Package B • Comal Springs Riffle Beetle Connectivity (#132) Food source refinement (#130) Rationale: o o Package C Both these studies build off the foundation of changes in flow that result in vegetation decay, water quality changes, and invertebrate response. Both studies will involve laboratory experiments at the Freeman Aquatic Building (FAB) in which water quality parameters will need to be altered. Setting up heating, chilling, CO 2 depletion, etc. is no trivial endeavor and thus having one reservoir chamber to support the water quality conditions simultaneously for both these studies will save space and money. Both these studies involve teaming with Dr. Nowlin at Texas State University and laboratory components at the FAB so costs associated with travel to and from the FAB could be reduced from an efficiency standpoint. Algal Dynamics and DO depletion (#134) Ludwigia interference (#137) Comal Springs Riffle Beetle Connectivity (#132) Food source refinement (#130) Rationale: o All four of these studies have interdependencies including field components, laboratory components, project team members, and travel as described above. Should the BIO-WEST project team be selected for all four, an additional 5% discount would be applied. Based on our approaches we are confident the above packages would save time, space, and resources. DEGREE OF COMPLIANCE - All services quoted in this proposal are in full accord with the general requirements of proposal #132-14-HCP. The BIO-WEST project team has all the resources necessary to perform the services outlined in the RFP. PROPOSAL PRICING - The cost estimate for the Comal Springs Riffle Beetle Habitat Connectivity Study (#132-14-HCP) has been submitted under separate cover. 10 BIO-WEST Riffle Beetle Habitat Connectivity Study ATTACHMENT A1 ATTACHMENT A1 EDWARDS AQUIFER AUTHORITY PROPOSAL SUMMARY FOR #132-14-HCP COMAL SPRINGS RIFFLE BEETLE HABITAT CONNECTIVITY STUDY Vendor Name: Submit to: BIO-WEST, Inc. Ms. Cyndi Holman Procurement Specialist Edwards Aquifer Authority 900 E. Quincy Street San Antonio, TX 78215 Attach your proposal to implement Applied Research related to study algae dynamics and dissolved oxygen depletion to this document. The following exceptions to the Specifications are noted: None Response to Commercial Questions and Statements What is the current financial status and condition of the proposing entity? BIO-WEST’s current financial status is strong. The EAA reserves the right to request financial statements as needed. Please mark one of the following: _____X____ I agree with the terms and conditions of the contract form as contained in Attachment C to the proposal specifications. _________ I do not agree with the terms and conditions of the attached contract form as contained in Attachment C to the proposal specifications. A1-1 11 BIO-WEST Riffle Beetle Habitat Connectivity Study ATTACHMENT A3 ATTACHMENT A3 EDWARDS AQUIFER AUTHORITY CONFLICT OF INTEREST STATEMENT #132-14-HCP COMAL SPRINGS RIFFLE BEETLE HABITAT CONNECTIVITY STUDY Vendor Name: BIO-WEST, Inc. DEFINITION A Conflict of Interest arises when a Consultant has a financial interest, person relationships or circumstances that could impair the Consultant’s ability to provide impartial advice or assistance in the best interest of the EAA or; the Consultant’s objectivity in performing the contract work is, or might be, impaired and/or; would cause the Consultant to have an unfair competitive advantage in the selection process. STATEMENT I hereby represent and warrant that providing the services described in this proposal will not constitute an actual or potential conflict of interest, and represent and warrant that it will not reasonably create even the appearance of impropriety. I further agree to: • • • Disclose any current employees who are former employees of the EAA; Represent and warrant that BIO-WEST has not given, nor intend to give, at any time hereafter, any economic opportunity, future employment, gift, loan, gratuity, special discount, trip, favor or service to an employee or representative of the EAA in connection with this solicitation; Represent and warrant that BIO-WEST shall not engage in providing consultation or representation of clients, agencies or firms which may constitute a conflict of interest which results in a disadvantage to the EAA or a disclosure which would adversely affect the interests of the EAA. The undersigned certifies that the information contained in this letter is true to the best of their knowledge. A3-1 13 BIO-WEST Riffle Beetle Habitat Connectivity Study SCOPE OF WORK As shown in the RFP, this study has been broken into five tasks including 1) Literature Review, 2) Methodology Development, 3) Conduct Applied Research, 4) Draft and Final Reports, and 5) Meetings and Presentations. The overall objective of our proposal is to evaluate data and organism responses that may be useful in management decisions and may be necessary for the development of a Comal Springs riffle beetle component of the HCP Ecological Model. The distribution of the Comal Springs riffle beetle (Heterelmis comalensis) in central Texas and within the Comal system is well defined. Additionally, 2014 HCP applied research activities currently in progress are projected to provide important information on population size and preliminary responses to low-flow and altered water quality (water temperature and dissolved oxygen) conditions. Unfortunately, very little else is known regarding the life history and surface / subsurface interactions and how that affects the population and/or distribution of H. comalensis. As discussed in the introduction, Applied Research in 2013 and 2014 demonstrated that aquatic vegetation as fountain darter habitat and fountain darters themselves are quite tolerant suggesting that H. comalensis may in fact be more of a sentinel species than the fountain darter in the Comal system. This is extremely important in that the present HCP flow regime exhibits periods of extended drying of the spring runs, and areas along the western shoreline and Spring Island (these areas are the presumed strong hold for the riffle beetle in the Comal System). Although the HCP flow regime is not projected as severe on the minimum end as experienced in the drought of record (DOR), these projected HCP extended periods of < 100 cfs are well beyond what was observed in the DOR. With H. comalensis likely shifting to be the sentinel species for the Comal system, understanding their tolerance and surface/subsurface interactions is vital to making Phase II decisions. Both H. comalensis HCP applied research projects (low-flow and plastron) conducted in 2014 used aquatic invertebrate surrogates (Microcylloepus pusillus, Heterelmis vulnerata, and Heterelmis glabra) at differing levels for various reasons. The use of a surrogate species in place of H. comalensis would be extremely useful, but after a series of trials in 2014 there were concerns raised regarding a few of the aforementioned species. Additionally, some issues with the survival of H. comalensis in a laboratory setting were experienced in 2014. This latter phenomenon provides the rationale for the first task associated with this scope of work. The concept of more detailed testing of surrogates prior to future use is addressed in the Scope Alterations section below. Task 1 – Literature Review: The BIO-WEST project team will conduct a detailed literature review including at a minimum the categories recommended in the RFP. The BIO-WEST project team has the direct advantage in that we completed previous Applied Research efforts directly for the HCP dealing with aquatic macroinvertebrate tolerances. As such, should our team be selected, much of the literature has already been mined and reviewed allowing our project team to be ready to move quickly into methodology development. An example of literature pulled together by the project team in support of this proposal is presented below. Task 2 – Methodology Development: Similar to the literature review, the BIO-WEST project team has already conducted a number of trials in 2013 and 2014 to evaluate Hyalella azteca, H. comalensis, M. pusillus, and H. glabra. This was no small endeavor and provides our team a direct advantage over other researchers. Additionally, the BIOWEST project team also has extensive experience preparing methodologies, experimental design, and 15 BIO-WEST Riffle Beetle Habitat Connectivity Study presenting to the HCP science team as discussed in the executive summary. We presented and received approval for all nine study designs from the HCP science committee in 2013 and 2014 with minimal revisions requested. The experience of how to streamline and communicate effectively with the HCP science team is a key aspect in the success of these projects. Task 3 – Conduct Applied Research: In 2015, we are proposing a series of trials involving H. comalensis and the surrogate species as follows: Phase 1: - Pre-laboratory Trial – TSU Freemen Aquatic Building (FAB) versus USFWS San Marcos Aquatic Resource Center (SMARC) or similar facility water comparison trial Because of the issues experienced with H. comalensis and H. vulnerata in 2014, we feel it is prudent to conduct a preliminary surrogate evaluation with water sources from two independent facilities. One hypothesis is that there might be infrequent and unpredictable pulses of “bad” water in the FAB system, but trying to capture such an event with high resolution chemical analysis is both costly in terms of time and money. Preliminary data indicates that the incoming water at the FAB doesn’t change much at all, but that data is logged in the Artesian Well at 15 minute intervals and this site is upstream from the tank that supplies water to the living streams. Water in the storage tank that supplies the living stream systems has been analyzed for cations and anions on a couple of occasions with no abnormalities noted. Until comparative testing is done biologically with these known water sources, a definitive answer of whether the water source in FAB is associated with increased mortality of H. comalensis in the FAB remains unknown. 16 BIO-WEST Riffle Beetle Habitat Connectivity Study Experimental Design: • Collect 30 to 60 individuals of each H. comalensis, M. pusillus, H. vulnerata and H. glabra. • Set up six identical aquaria with flow through at the same water rates and place three aquaria at the SMARC (or similar facility) and three at the FAB. • Place 5 to 10 beetles of each of the 4 species (thus 20 to 40 beetles total) into each of the six tanks. • Place thermisters for continuous water temperature measurements in each of the six treatment tanks. • Monitor standard water quality parameters daily – dissolved oxygen (DO), ph, conductivity and water temperature of the effluent of each of the six trial tanks. • Install In situ TROLL water quality logging data sondes in reservoirs with the incoming water sources at both the SMARC (or similar facility) and FAB. The sondes will continuously log temperature, DO conductivity, and ORP (oxidation-reduction potential). • Run trial for 4 weeks checking survival bi-weekly throughout. This preliminary trial serves two purposes. First it assesses water chemistry at two independent facilities for a period of time that a typical invertebrate experiment would not exceed. Secondly, it examines if H. comalensis and several potential surrogate species exhibit differential survival. If survival occurs and is not significantly different, all experimentation will be carried forward at the FAB. If not, arrangements will be made to work at the SMARC or a similar facility known to support aquatic invertebrates. Regardless of study results, additional surrogate testing is highly recommended as discussed in the Scope Alterations section of this proposal. Phase 2: Detailed Surrogate Testing – see Scope Alteration section below. Phase 3: Riffle Beetle Habitat Connectivity Trials We propose a series of trials to test surrogate and/or H. comalensis behavior in the Riffle Beetle Aquifer Simulation System (RBASS) that was designed, built and used by BIO-WEST for 2014 research (pictured to the right). We highly recommend the surrogate testing (Phase 2) and use of surrogates for all initial testing to increase sample sizes, minimize impacts directly to H. comalensis, and ease any potential permitting conditions of USFWS Ecological Services. If Phase 2 is conducted, this should provide the information to move forward with confidence with a surrogate. If Phase 2 is not conducted, the formal experiments described in Phase 3 will need to be run with H. comalensis and likely much smaller sample sizes. Pending the results of Phase 1, all studies with H. comalensis may also need to be conducted at the SMARC or similar facility. Regardless of beetle used, the series of tests remains the same and are 17 BIO-WEST Riffle Beetle Habitat Connectivity Study summarized as follows. Of course all final methodologies and experimental designs will need to be vetted with the HCP Science Committee for comment. TRIAL A - SPRING RUN HABITAT CONNECTIVITY The goal of this study is to assess the importance of subsurface habitat connectivity to the surface edge hypothesized as being important to H. comalensis in terms of protective habitat, food or both. The objective would be to examine the influence of surface connectivity to edge habitat in a series of simulated spring runs. This is a test of lateral connectivity of the springs runs to the bank. It is anticipated that this would be evaluated via a connection with course particulate organic matter (CPOM) and organic matter exudate type materials along the edge of the habitat (bank). Within the RBASS spring run design (pictured below), this would involve distributing this material in the upper few centimeters (edge) of the experimental set-up and then passing water laterally through the substrate column. It is anticipated that up to nine experimental spring run units would be used to test up to three treatments in triplicate. The treatments may include: 1) Constant water level and flow (control) with surface edge habitat connected at all times 2) Reduced water level with no connectivity to surface edge habitat 3) Reduced water level with supplemental connectivity to surface edge habitat 18 BIO-WEST Riffle Beetle Habitat Connectivity Study The number and type of beetles used per treatment would be determined upon completion of Phase 2 surrogate testing. It is anticipated that the trial would be run for a minimum of three weeks with daily water quality measurements recorded. If surrogates are used, survival will be the parameter recorded at weekly intervals over the course of the study. If H. comalensis are used, ecological death may be the parameter tested. Ecological death is determined when the beetle ceases to respond to agitation but is able to recover when placed back into its original holding chamber. Analysis of variance (ANOVA) will be used to evaluate any differences among treatments for survival or ecological death at the conclusion of the study. TRIAL B - UPWELLING HABITAT CONNECTIVITY Trial B is similar in concept to Trial A, but involves the testing of vertical flow rather than horizontal flow described in Trial A. The objective of this study is to assess vertical flow and connectivity whether on the way up (during normal discharge and upwelling conditions) or on the way down (during low water levels via either rainfall or water supplementation). The hypothesis is that the surface layer (edge included) is important to the Comal Springs riffle beetle for cover, food or both. It is anticipated that this trial would be conducted in the upwelling chambers of the RBASS (pictured below) with water percolating through substrates from above (with CPOM in the upper layers). It is anticipated that up to twelve experimental upwelling units would be used to test up to four treatments in triplicate. The treatments likely include: 1) Constant upwelling flow (Control) with surface layer connection at all times, 2) Reduced water level (subsurface) with no connectivity to surface layer, 3) Reduced water level (subsurface) with supplemental (man-made) but constant connection to surface layer, and 4) Reduced water level (subsurface) with intermittent connection (simulated rain fall) to surface layer. 19 BIO-WEST Riffle Beetle Habitat Connectivity Study As with Trial A, the number and type of beetles used per treatment would be determined upon completion of Phase 2 surrogate testing. It is anticipated that the trial would be run for a minimum of three weeks with daily water quality measurements (temperature, DO, conductivity) recorded. Depending on whether surrogates or H. comalensis are used, survival or ecological death would be recorded at weekly intervals over the course of the study. Statistical analysis would be used to evaluate survival or ecological death amongst upwelling treatments at the conclusion of the study. TRIAL C - FIELD VALIDATION - HABITAT CONNECTIVITY Upon completion of the series of laboratory studies in surface water chambers and the RBASS, we propose to conduct a validation field exercise in Spring Run 3 (pictured to the right and below) of the Comal system. At this point, it is difficult to describe specifics for this field effort as it is dependent on the results of the laboratory trials. However, it is anticipated to include natural areas that are connected to surface habitat as the control and areas that are disconnected (pictured below) as the test unit. An examination of H. comalensis use will be conducted in those areas before and after connectivity (via well water or recirculated water from Spring Run 3 or Landa Lake) with flow over natural detritus/roots and then statistically compared back to the control. 20 BIO-WEST Riffle Beetle Habitat Connectivity Study Assumptions: Adequate space and equipment will be made available as requested at the FAB. This includes three living streams for experimentation. The USFWS will allow for testing in Phase 1 and subsequent phases (if necessary) to be conducted at the SMARC. If not, other facilities using Edwards Aquifer water known to support aquatic invertebrates will be pursued. Task 4 – Draft and Final Reports: For costing purposes, this task includes all data reduction, data analysis, and statistical interpretation. As outlined in the RFP, BIO-WEST will submit the draft project report no later than thirty (30) days from the conclusion of the research. The draft report will meet the requirements of the RFP. A final report will be submitted within 2 weeks following the review of EAA’s review comments. Task 5 – Meetings and Presentations: As referenced in the RFP, BIO-WEST will attend a minimum of two meetings and prepare and conduct one presentation to the HCP Science team. As BIO-WEST will be attending the HCP Science meeting for other ongoing activities, should our team be selected we will attend this meeting at NO charge to this project. PROPOSED SCOPE ALTERATIONS: Phase 2 – Detailed Surrogate Trial – The study location would be dependent on the results of Phase 1. If there is no difference between the water sources at the SMARC or similar facility and FAB, all work would be conducted at the FAB. However, if we determine if there are significant differences in survivorship for H. comalensis and the surrogate species, then all subsequent testing will be conducted at the facility with higher survivorship. Regardless, all work would be built upon 2014 activities and 2015 Phase 1 results. At this point, water temperature and DO tolerances for H. glabra and H. vulnerata are being studied at the FAB as part of the plastron study. If the Authority approves an extension to the plastron study and both the Authority and USFWS allow for a location shift to the SMARC, those surrogate results will be duplicated via short-term experiments on H. comalensis. Either way, by the end of the 2014 applied research, we should have an idea as to any differences in temperature and DO tolerances in terms of two or three of the species (and presumably whether the two other Heterelmis spp. are suitable surrogates for H. comalensis) relative to those two parameters. However, we don’t have any information about M. pusillus or the preferences/performance of all the species on different substrates, flow rates, etc. We do not see a need to repeat experiments on short-term exposure of water temperature or DO, unless H. comalensis was not able to be tested against in 2014. In that case we recommend a short-term repeat of that effort including all 4 species described above. Our main recommendation is a series of experiments where we test longer term responses of experimental replicate populations (n=10 individuals per population x 4 populations of each beetle species) under regimes of different temperatures (in situ temperature, 25°C and 30°C) and different substrate types, as well as possibly horizontal and upwelling flow. Our intent would be to use a crossclassified design on this so we could pull out the independent effects of each factor and their interaction on beetle survival and/or ecological death. The objective of this series of experiments is to pinpoint a surrogate that is scientifically defensible and not just based on expert opinion. Using a surrogate would undoubtedly allow larger sample sizes to be 21 BIO-WEST Riffle Beetle Habitat Connectivity Study tested moving forward as well as less complex, and shorter delays in permitting. All costs for Phase 2 were included separately under “Other” on Attachment A2. Therefore, should the Authority decide not to fund this supplement, you can simply deduct that cost from the total. QUALIFICATIONS - PROJECT TEAM Established in 1976, BIO-WEST, Inc. is a multi-discipline, environmental consulting firm with a permanent core staff of senior level scientists and an experienced support staff. The firm is a leader in environmental consulting and problem solving, and has earned a widely acknowledged reputation for providing objective, credible services and superior products to a wide variety of agencies, organizations, and private clients. BIO-WEST strives to maintain an outstanding reputation for fisheries, aquatic vegetation, and endangered species investigations throughout the United States. BIO-WEST has extensive knowledge of the aquatic biota and ecological relationships of the Comal and San Marcos River/Springs ecosystem as they have conducted continuous monitoring and research related to the endangered species in these systems for over 13 years. Finally, BIO-WEST has all state and federal endangered species permits necessary to conduct this work upon award. The Department of Biology at Texas State University - San Marcos is a large, multidisciplinary department with many strong research programs that are well funded. They serve about 14,000 students each year in modern facilities with up to date instrumentation emphasizing inquirybased hands-on instruction. The Department of Biology’s goal is to attain national and international prominence through integrating undergraduate and graduate education with multidisciplinary research programs. Dr. Weston Nowlin (Associate Professor – Texas State University) is an expert at assessing how biotic and abiotic factors affect the abundance, distribution, and diversity of organisms in ecosystems. The Nowlin Lab is highly collaborative and students represent a variety of research interests. Students often assist each other on projects and have a great deal of fun in the lab and the field. Zara Environmental LLC (Zara) is a woman-owned business founded in 2003 by Dr. Jean Krejca and Peter Sprouse. Zara staff have a combined 80 years experience in surveying for aquifer and springassociated invertebrate species, and they employ six scientists permitted for work with the Comal Springs Riffle Beetle. Zara’s experience not only includes surveys at such known localities as Hueco Springs, Comal Springs, Barton Springs, Fern Bank Springs, Salado Springs, Rattlesnake Cave, Ezell's Cave and Jacobs Well, but also at several less well known springs, water caves and wells throughout the Edwards Plateau. Since 2007, Zara has documented more than 20 new locality records for spring and aquifer dwelling crustaceans, documenting an entire order of fauna previously unknown to the Edwards Aquifer, expanded the known range of two species of aquifer dwelling blind catfish, and collected new species of spring and aquifer dwelling copepods and amphipods. Zara personnel have also conducted aquatic surveys for invertebrates in surface and subsurface environments throughout Bell, Bexar, Comal, Coryell, Guadalupe, Hays, Travis, and Williamson counties.. 22 BIO-WEST Riffle Beetle Habitat Connectivity Study The Center for Reservoir and Aquatic Systems Research (CRASR) is a research and education partnership between Baylor University and the City of Waco focused on aquatic resources. Dr. Robert Doyle is the Director of the CRASR and he and his students continue to explore novel ideas and creative techniques to maximize aquatic vegetation research and restoration in spring ecosystems. KEY TEAM MEMBERS Edmund L. Oborny, Jr. – Project Principal Mr. Oborny is the Fisheries Section Leader and BIO-WEST’s VicePresident. He specializes in aquatic ecology, threatened and endangered species, water quality, biological modeling, and instream flow issues and concepts. He has 19 years of professional extensive experience and expertise with fisheries investigations throughout Texas and the western United States. Mr. Oborny has been the project manager and principal aquatic resources investigator for the multi-discipline, multi-year Variable Flows and Water Quality Study (now HCP Bio-monitoring) for the Edwards Aquifer Authority since its inception. This large-scale applied research project involves intensive sampling, data analyses and interpretation regarding the importance of various flow regimes and associated impacts to the threatened and endangered species of the Comal and San Marcos Springs/River ecosystems. Mr. Oborny’s experience and expertise with complex ecological issues is also illustrated by the number of professional appointments that he has received in the past 5 years. These appointments are listed in their entirety in Mr. Oborny’s resume, but are summarized below: State wide Environmental Flows Science Advisory Committee Member: 2009-present Southern Edwards Aquifer Species Recovery Team Member: 2008-present. Special Consultant to the Biological Working Group (BWG) in Spring Valley, Nevada: 2007 – present. Blue Ribbon Science Advisory Panel Member (Aquatic Resources) in Owens Valley, California: 2009-present. Edwards Aquifer Recovery Implementation Program (EARIP) Science Subcommittee Member: 2008-2011. Third Party Independent Review of Mono Basin Stream Ecosystem Flows Recommendations: 2009. Edwards Aquifer Recovery Implementation Program (EARIP) Biological Modeling Team: 2008 – 2010. 23 BIO-WEST Riffle Beetle Habitat Connectivity Study Dr. Weston Nowlin: Texas State University – Co- Principal Investigator Dr. Nowlin is an Associate Professor of Biology at Texas State University-San Marcos, specializing in aquatic ecosystems. Research in his is centered on the role of organisms and communities in affecting ecosystem-level processes like nutrient cycling and organic matter flows. In addition, Dr. Nowlin assesses how biotic and abiotic factors affect the abundance, distribution, and diversity of organisms in ecosystems. In order to address these research areas, his lab utilizes a variety of methods and research approaches, including nutrient biogeochemistry, whole ecosystem metabolism and material flux studies, and the use of stable isotopes. Additionally, research in Dr. Nowlin’s lab takes place in a variety of ecosystem types and at a diversity of scales. They conduct research in wetlands, streams, rivers, lakes, and reservoirs as well as small laboratory-based studies, field-based experimental systems, and at the wholeecosystem scale. Dr. Nowlin is a Co-Principal Investigator on the ongoing Comal Springs Riffle Beetle plastron study funded as part of the 2014 HCP Applied Research. Mr. Randy Gibson: Senior Macroinvertebrate Specialist Mr. Gibson specializes in aquatic macroinvertebrates and is the foremost expert on the Comal Springs riffle beetle (Heterelmis comalensis). He has 15 years of professional experience working as both a fish biologist for the U.S. Fish and Wildlife Service, Aquatic Resource Center, San Marcos, Texas and as a Senior Macroinvertebrate Specialist for BIOWEST. Mr. Gibson conducts research and maintenance of captive populations of threatened and endangered species including the Comal Springs riffle beetle, Peck’s cave amphipod (Stygobromus pecki), fountain darter (Etheostoma fonticola), San Marcos salamander (Eurycea nana), Texas blind salamander (Typhlomolge rathbuni), and Texas wildrice (Zizania texana). Mr. Gibson’s professional duties include experimentation with how best to develop refugia for the Comal Spring riffle beetle to support its survival in captivity. Additionally, Mr. Gibson has been working for BIO-WEST since 2001 and is the lead investigator in charge of all endangered macroinvertebrate sampling involving cotton lures and drift netting over spring orifices. 24 BIO-WEST Riffle Beetle Habitat Connectivity Study Dr. Jean Krejca: (Zara Environmental) Dr. Krejca, President, Zara Environmental LLC, has extensive experience with the identification and regulation of endangered species and habitat in Texas. She is a specialist in the biology of subterranean species including karst invertebrates, bats, and Eurycea salamanders. She has been responsible for the design, implementation, and management of time sensitive environmental projects dealing with karst and aquatic issues in Bexar, Travis, Williamson, and Hays counties in central Texas. Dr. Krejca’s project experience also includes leading biological inventories, rare and endangered species surveys, and developing customized biological studies. She has worked as an invertebrate biologist since 1991 and she holds a USFWS permit for Comal Springs riffle beetle and other Comal Springs species. Dr. Robert Doyle: Baylor University – Co-Principal Investigator Dr. Robert Doyle currently serves as the Director for the Center for Reservoir and Aquatic Systems Research, Professor, and Department Chair in Biology at Baylor University. Dr. Doyle has nearly 30 years of experience specializing in aquatic plant ecology and community dynamics. Dr. Doyle has worked in the Comal and San Marcos rivers since the 1990s when he first completed aquatic vegetation maps for both systems. Dr. Doyle subsequently conducted numerous experimental plantings of native species in both the Comal and San Marcos River. Like Mr. Oborny, Dr. Doyle also serves on the USFWS Recovery Team for the endangered species in the Comal and San Marcos ecosystem. Dr. Doyle’s extensive knowledge of aquatic vegetation in the Comal River, and unparalleled expertise in aquatic vegetation applied research in spring ecosystems provides an outstanding compliment to the project team. 25 BIO-WEST Riffle Beetle Habitat Connectivity Study RELEVANT EXPERIENCE EARIP HCP 2014 Sponsored - APPLIED RESEARCH STUDY Comal Springs Riffle Beetle Low-Flow Study Objective: During a comprehensive literature review, there were no studies found about prior experiments where flow rates were used to predict invertebrate survival, particularly in subterranean habitats. The goal of this study was to use a novel experimental design to create “spring upwelling” mesocosms in an attempt to shed light on H. comalensis survivorship inside of the springs during periods of low flow and flow cessation. Vertical flow regimes were designed to mimic periods of drought that have caused Comal Springs discharge to decrease to the point that spring upwellings no longer connect the subterranean and surface habitats that H. comalensis likely inhabit in the wild. The objective was to provide information as to how the physical (i.e. temperature) and chemical properties (i.e. DO, pH, conductivity) of spring water change as flow velocity decreases and how beetle survivorship is related to these properties. The results of both will be valuable to inform EARIP HCP Phase II decisions. Task Description: Following the preliminary decisions about the construction of the mesocosms in substrate preferences, and surrogate responses, testing of the effect of flow and food availability on H. comalensis adults was conducted using a factorial experimental design. Each factor (flow and food availability) will have two levels (Yes or No) and the combinations will be tested Location: This study was conducted at the Texas State University Freeman Aquatic Building. Data Analysis and Results: Preliminary results show that surrogate invertebrate responses may not be applicable to H. comalensis relative to each treatment. Therefore, additional testing is underway to continue to explore these relationships. Upon completion of the formal study, the data collected will be analyzed using a factorial ANOVA, which will test the independent and interactive effects of flow and food availability in each treatment. 26 EARIP HCP 2013 Sponsored - APPLIED RESEARCH STUDY Low-flow Food Source Study BIO-WEST Riffle Beetle Habitat Connectivity Study Objective: Several EARIP HCP studies have focused on what happens to aquatic vegetation (a key fountain darter habitat) during low-flow. However, this study was conducted to address, “What will happen to invertebrate food sources for the fountain darter under extreme low-flow conditions?” Therefore, the objective of this study was to use native Hyalella azteca from the Comal system as a surrogate to evaluate this food source questions. The results of this study will be valuable to inform the ecological model being prepared for the EARIP HCP. Task Description: The study used a series of trials to build upon each other and address the question. The first was a series of pre-trial experiments to establish an appropriate acclimation process for this species. The next step was to conduct a series of Critical Thermal Maximum (CTM) experiments evaluating three different acclimation temperatures, and concluding with a determination of ecological death. Finally, both a constant test laboratory study evaluating different water temperatures in fiberglass living streams is being conducted, as well as a pond study that evaluates diurnal conditions of water quality and response of H. azteca in a low-flow and no-flow treatments in a pond environment. Location: This study was conducted at the aquatic nursery/greenh ouse/pond facilities of the San Marcos Aquatic Research Center. Data Analysis and Results: Data analysis involved evaluating survival, biomass, and morphology of the three species (Cabomba, Ludwigia, and Sagittaria) of plants being tested. Results from the study will examine the ratio of above ground to below ground biomass and relative growth rate of the plant species. Preliminary results suggest plants in both treatments grew over the course of the experiment. Statistical analysis is now underway to investigate differences among variables. 27 BIO-WEST Riffle Beetle Habitat Connectivity Study EARIP HCP 2013 Sponsored - APPLIED RESEARCH STUDY Vegetation Tolerance Laboratory Trials Objective: Submerged aquatic vegetation provides extremely important habitat for the endangered fountain darter. Therefore, understanding factors that influence aquatic vegetation growth and reproduction is critical to maintaining fountain darter populations. Objectives of this study were to evaluate the effects of low-flow conditions on aquatic vegetation survival and growth. Task Description: This study was conducted in a series of experiments starting with pre-study trials in aquaria, two sets of laboratory studies using fiberglass living streams, and concluding with a pond study. All experiments were aimed at defining parameters or establishing bounds for aquatic vegetation growth or decay that could be used to parameterize the HCP Ecological Model. Location: This study was conducted at the aquatic nursery/greenhouse/pond facilities of the San Marcos Aquatic Research Center. Data Analysis and Results: The response variables for the aquatic plant species are survival, biomass (above and below ground), and stem length and number. Results from pre-trial study showed that growth of plant species still occurred at elevated water temperatures and limited CO 2 conditions. This information was used to design both a constant test laboratory study evaluating three different water temperatures and low CO 2 combinations with four aquatic plant species, as well as a pond study that evaluated diurnal conditions of water quality in a low-flow and no-flow treatment in a pond relative to both Ludwigia and Vallesneria. Results from each study will be presented in fall 2013. O 2 concentrations were tested. 28 BIO-WEST Riffle Beetle Habitat Connectivity Study EARIP HCP 2013 Sponsored - APPLIED RESEARCH STUDY Bicarbonate Utilization Potential - pH Drift Study Objective: Submerged aquatic vegetation provides extremely important habitat for the endangered fountain darter. Therefore, understanding factors that influence aquatic vegetation growth and reproduction is critical to maintaining fountain darter populations. Objectives of this study were to determine which aquatic plant species are capable of HCO3 utilization. Task Description: This approach was to assay plants under closed-system conditions and see how far they can “push” pH. If a CO 2 obligate: Ps stops when CO 2 is depleted. However, if a HCO 3 user: Ps continues to higher pH. As with the other aquatic vegetation experiments, the pH drift study was designed to define parameters and establish bounds for aquatic vegetation growth or decay that can be used to parameterize the HCP Ecological Model. Location: This study was conducted at the Center for Reservoir and Aquatic Systems Research facility on the campus of Baylor University. Data Analysis and Results: This study used a synthetic Ps solution (known alkalinity and carbon composition). As CO 2 depletion lower total inorganic carbon (C T ) only modestly, HCO 3 use lowers C T more strongly. The analysis focused on the major Comal aquatic plant species: Hygrophila, Ludwigia, Cabomba, Vallisneria, Sagittaria and bryophytes. The Key response focus was on final pH and C T :Alk ratio. The “better” a species can use HCO 3 , the higher they can push (drift) pH and the lower the C T :Alk ratio. A one-way ANOVA was used to compare among species. Final Results from the pH drift study will be presented in fall 2013. 29 BIO-WEST Riffle Beetle Habitat Connectivity Study EARIP HCP 2013 Sponsored - APPLIED RESEARCH STUDY Laboratory vs. Field Study Objective: Submerged aquatic vegetation provides extremely important habitat for the endangered fountain darter in both the Comal and San Marcos ecosystems. Therefore, understanding factors that influence aquatic vegetation growth and reproduction is critical to maintaining fountain darter populations. As not all low-flow conditions will be observable in the field, laboratory studies are going to be needed to simulate field conditions. Therefore, the objective of this study was to compare growth of aquatic vegetation in the field versus the laboratory while grown under similar environmental conditions. The results of which will be valuable to inform the ecological model being prepared for the EARIP HCP. Task Description: The study approach involved growing plants in the field and laboratory under as close to the same environmental conditions. Environmental conditions tested included flow velocity, temperature, carbon dioxide, pH, conductivity, light, and depth. Location: This study was conducted at the aquatic nursery/greenhouse facilities of the San Marcos Aquatic Research Center and within the Old Channel of the Comal River. Data Analysis and Results: Parameters pH Alkalinity, total Kjeldahl-N Nitrate-N/IC Nitrite-N/IC Total Nitrogen Total phosphorus Units pH units mg/L mg/L mg/L mg/L mg/L mg/L ARC - Reservoir 4/9/2013 5/6/2013 7.8 260 0.434 1.67 <0.02 2.11 0.0619 7.9 260 0.278 1.6 <0.02 1.88 <0..02 Old Channel 4/9/2013 5/6/2013 7.8 230 0.161 1.72 <0.02 1.88 <0.02 7.6 240 0.404 1.67 <0..02 2.08 <0..02 Data analysis involved evaluating survival, biomass, and morphology of the three species (Cabomba, Ludwigia, and Sagittaria) of plants being tested. Results from the study will examine the ratio of above ground to below ground biomass and relative growth rate of the plant species. Preliminary results suggest plants in both treatments grew over the course of the experiment. Statistical analysis is now underway to investigate differences among variables. 30 BIO-WEST Riffle Beetle Habitat Connectivity Study Comal and San Marcos Springs Variable Flow Study (2001 -2012) – now HCP BIO-MONITORING PROGRAM (2013-2014) Beginning in 2001, BIO-WEST contracted with the Edwards Aquifer Authority (EAA) to conduct a multi-year applied research effort with the goal of augmenting the available data on population dynamics of threatened and endangered species in the San Marcos and Comal Rivers/Spring Ecosystems and its relationship to springflow. Over the past 14 years, BIO-WEST biologists have collected and analyzed data on a variety of components within these systems including: aquatic vegetation, exotic species, water quality, Texas wild-rice, fountain darters, salamanders, and invertebrates. This study incorporates regular quarterly sampling in several locations in the two systems, with flow dependent sampling conducted when the discharge in one or both of the rivers falls below or rises above specified “trigger” levels. The primary goal is to establish baseline data for each of the endangered species’ populations and track habitat usage and population dynamics as spring flow decreases during drought conditions. Specialized techniques such as drop netting, drift netting, in-situ water temperature loggers, and SCUBA surveys, are being used to sample these unique ecosystems. Through this extensive multi-component monitoring program BIO-WEST personnel have become very familiar with the Comal River/Springs Ecosystem (in particular the Upper Spring Run reach and Landa Lake) and the ecological interactions which influence endangered species and their habitat. In addition to each of the study components described above, several individual research efforts that have developed during the course of this study were conducted by BIO-WEST in conjunction with the USFWS ARC. Special studies are noted with brief descriptions below: 31 BIO-WEST Riffle Beetle Habitat Connectivity Study EARIP HCP 2014 Sponsored - APPLIED RESEARCH STUDY Fountain Darter Movement Study Objective: Fountain darters, like other darters, appear highly sedentary, moving on average 10 m within a year and up to 95 m within 26 days under a stable hydrograph (Dammeyer et al. 2013). When movement occurs, fountain darters move among habitats more frequently (51%) than other darters (3 to 20%; Mundahl and Ingersoll 1983; Labbe and Fausch 2000), most often towards low growing vegetation, upstream, and during the winter and spring-summer seasons. Determining how and why fountain darters disperse throughout the Comal River system could be vital to the conservation of this species. Task Description: A mark and recapture study was conducted to determine how movement of fountain darters is affected by habitat and temperature changes under low flow conditions. Fountain darter mark and recapture techniques utilized methods previously developed for darters and other small-bodied fishes, with visual implant elastomer (VIE) as the marking material. Both visual (re-sight) and physical (dipnet, recapture) methods were used for relocating fountain darters due to their habitat affinity (i.e. benthic fish occupying areas of dense vegetation). Location: This study was conducted in the Upper Spring Run reach of the Comal River as well as in Blieder’s creek just above the confluence with the Comal system. Data Analysis and Results: Distribution of individuals up or downstream of cohort release points are being evaluated by comparing distributions using Kolmorogov-Smirnov tests. The effect of temperature on fountain darter movement is being assessed by comparing the number of movements toward or away from areas of higher temperature over each recapture interval using chi -square tests. 32 BIO-WEST Riffle Beetle Habitat Connectivity Study EARIP HCP 2014 Sponsored - APPLIED RESEARCH STUDY Fountain Darter Predation Study The objective of this study was to begin the process of understanding complex predatorprey relationship in the San Marcos and Comal rivers. A series of laboratory experiments were conducted to assess the behavior of fountain darters with and without predators; quantify predation rates of fountain darters with invertebrate and vertebrate predators; and evaluate how aquatic vegetation and substrates might mediate predator consumption of fountain darters. The Objective: results will be valuable to inform the ecological model being prepared for the EARIP HCP. Task Description: The study approach involved unifying predator/prey interactions under one experimental design. The experimental unit was an aquarium with fountain darters. The dependent variable was the numbers of darters partially or completely consumed. The control will be an experimental unit without a predator. Treatments included crayfish only, centrarchids only, and crayfish and centrarchids (with vegetation and without). Location: This study was conducted at the Texas State University Freeman Aquatic Building. Data Analysis and Results: A two-factor ANOVA was used to test for differences (α=0.05) among treatments and a Fisher’s LSD for post-hoc mean separation tests. Preliminary results show differences among predator treatments (control, crayfish only, bass only, and crayfish*bass) and suggest that crayfish and bass effects are additive. No evidence of trophic cascade effects. Statistical analysis is now underway to investigate these differences. 33 BIO-WEST Riffle Beetle Habitat Connectivity Study EARIP HCP 2014 Sponsored - APPLIED RESEARCH STUDY Fountain Darter Fecundity Study Objective: Given that low flow conditions will likely alter the physical habitats of the fountain darter, we predict that changes in physical habitats, especially low-growing and dense vegetation, will reduce the reproductive readiness and success of the fountain darter. To test this prediction, a baseline in fountain darter reproductive readiness among a gradient of flow regimes and among vegetation type was established. Objectives of this study were to quantify elements of fountain darter reproduction (gonadal recrudescence, ovarian development, fecundity, and oocyte maturation) among available flow gradients ranging from 10 to 120 cfs in the wild and among physical habitat types and substrates (open substrates, low-growing and tall-growing aquatic vegetation). The results of which will be valuable to inform the ecological model being prepared for the EARIP HCP. Task Description: The study approach involved collecting sexually mature (>24 mm) female fountain darters monthly. On each collection trip and when available, up to 5 female darters were taken from 3 to 5 areas of bare substrates (sand, gravel, cobble), short growing vegetation, and tall-growing vegetation within the San Marcos River and Comal River. In the laboratory, lengths and weights of each fish were taken. Gonadosomatic index, ovarian stage, fecundity, and oocyte maturation were determined for each fish following methodologies described specifically for darters. Location: This study was conducted within the Comal and San Marcos Rivers while laboratory work was conducted at the USFWS San Marcos Aquatic Research Center and Texas State University Freeman Aquatic Building. Data Analysis and Results: A multi-factored MANOVA will be used to assess differences (α=0.05) among treatment effects and response variables. Preliminary results suggest differences amongst habitat conditions. Statistical analysis is now underway to investigate these differences. 34 BIO-WEST Riffle Beetle Habitat Connectivity Study EAA Variable Flow Study - APPLIED RESEARCH Fountain Darter Lab Study Objective: Temperatures of 22-24°C are thought to be optimal for fountain darter reproduction, whereas temperatures 27°C and higher are known to negatively impact reproduction (Bonner et al. 1998). However, in the lower reaches of the Comal and San Marcos Rivers temperatures often fluctuate between optimal and suboptimal within a 24-hour period. This study was designed to test the impacts of such fluctuating water temperatures on fountain darter egg and larval production. In addition, this study examined the effects of the exotic trematode parasite Centrocestus formosanus on fountain darter reproduction. Location: This study was conducted at the San Marcos National Fish Hatchery and Technology Center by Dusty McDonald and Dr. Timothy H. Bonner of Southwest Texas State University with support from BIO-WEST. Task Description: Fountain darters (half infected with C. formosanus cercariae and half uninfected) were exposed to one of four temperature treatments on a 24-hour cycle: constant 24°C, fluctuating 24 to 26°C, fluctuating 26 to 28°C, and fluctuating 28 to 30°C. Total number of eggs, number of healthy eggs, and larval production were then compared between infected and uninfected fish and between temperature treatments. Trial 1 (220) 200 35 (64) 100 100 (28) Mean number of eggs (2) (0.5) (0) Trial 2 (68) 100 100 50 (13) (14) (4) (0) 150 50 (14) (0) (0) Trial 3 (94) 150 (99) (50) 100 (47) 100 (28) 50 50 (0) 24°C 24-26°C 26-28°C 28-30°C (0) 24°C Temperature treatments (0) 24-26°C 26-28°C 28-30°C Mean number of larvae Data Analysis and Results: Infection by C. formosanus at levels and durations tested in this study did not affect fountain darter reproductive success, and therefore, infected and uninfected fish were combined for temperature analysis. Total egg, healthy egg, and larval production were highest at a constant 24°C, but significantly decreased in variable higher temperature treatments. These laboratory results demonstrate that fountain darter reproductive success is affected by temperatures that fluctuate between optimal and suboptimal in a 24-hour period. However, data from field collections show successful reproduction and recruitment in wild populations when temperatures fluctuate up to 26°C. 200 (122) (111) BIO-WEST Riffle Beetle Habitat Connectivity Study EAA Variable Flow Study - APPLIED RESEARCH Aquatic Vegetation Lab Study Objective: Submerged aquatic vegetation provides extremely important habitat for the endangered fountain darter. Therefore, understanding factors that influence aquatic vegetation growth and reproduction is critical to maintaining fountain darter populations. Objectives of this study were to evaluate the effects of varying spring flows and resulting water quality parameters on the growth of several aquatic plant species which occur in the Comal and San Marcos Rivers, including endangered Texas wildrice Zizania texana. Task Description: This study was conducted in two phases. In Phase 1, Vallisneria sp. and Ludwigia repens plants in outdoor raceways were exposed to varying flows of Edwards Aquifer water. Under each flow level water quality parameters were closely monitored and growth of both species was measured at the end of the study and compared between treatments. In Phase 2, flow levels and temperature were held constant, and carbon dioxide (CO 2 ) concentrations were manipulated between treatments to examine effects on growth of Texas wild-rice, Ludwigia repens, Vallisneria sp., Hydrocotyle umbellate, Riccia sp., and Amblystegium sp. Location: This study was conducted at the aquatic nursery/greenhouse facilities of the San Marcos Zizania Below Ground Biomass DRY WEIGHT (g) 0.6 0.5 0.4 0.3 0.2 0.1 0.0 National Fish Hatchery and Technology Center. 1 2 3 CO2 TREATMENTS Data Analysis and Results: Results from Phase 1 of this study showed that growth of both aquatic plant species tested were greatest under higher flow conditions, mainly as a result of higher CO 2 concentrations. Therefore, in Phase 2 the impact of CO 2 concentrations were tested. When flow and temperature were held relatively constant most plants exhibited increased growth in the higher CO 2 treatment. In summary, dissolved CO 2 concentrations appear to be important in shaping the aquatic plant communities of the San Marcos and Comal Rivers, and therefore, play an important role in maintaining populations of Texas wild-rice and in maintaining quality habitat for fountain darters. 36 BIO-WEST Riffle Beetle Habitat Connectivity Study EAA Variable Flow Study - APPLIED RESEARCH Comal Springs Riffle Beetle Lab Study Objective: A paucity of data exists regarding physiological and ecological requirements of the Comal Springs riffle beetle (Heterelmis comalensis). To expand on data collected in the Comal Springs riffle beetle habitat survey, a laboratory study was conducted. Of particular interest is that this species must have persisted through low spring flow events such as the period in 1956 when Comal Springs ceased to flow for approximately five months. Potentially, riffle beetles migrate deeper into the substrate during low flow periods where some subterranean flow may still exist. This lab study was designed to test the relationship between substrate depth, vertical and lateral flow, and riffle beetle location. Vertical Distribution 100 Location: This study was conducted at laboratory facilities of the San Marcos National Fish Hatchery and Technology Center. upwelling 80 horizontal flow percent of beetles Task Description: In the lab, several detailed experimental blocks were constructed with black acrylic plexiglass and Bio Barrel polypropylene media. The 40 construction of this apparatus was designed to mimic the 20 natural gravel and cobble substrate of the riffle beetle and 0 allowed for movement through the media via interstitial spaces. Barrier layers were put in place to slow beetle movement and more accurately reflect the decreasing size of interstitial spaces deeper in the substrate, after test runs revealed that beetles were moving quickly to the bottom of the block. Blocks were designed to allow flow manipulation both horizontally and vertically through the apparatus. Riffle beetle locations were noted after experimenting with combinations of horizontal and vertical flows. 60 top horizontal Data Analysis and Results: In this laboratory study, Comal Springs riffle beetles displayed a tendency for downward movement through the substrate and a preference to be in, and move toward moving water (current). When horizontal flow was applied to the block, the beetles were most commonly found towards the front. When a vertical upwelling flow was applied to the bottom, most beetles were collected near or moved toward the bottom. Therefore, it is feasible that these beetles would respond to decreased spring flows by moving downward into the substrate in search of a flow stimulus. It is also likely that these beetles inhabit areas deeper in the gravel and sediment than have previously been sampled. 37 BIO-WEST Riffle Beetle Habitat Connectivity Study WORK SCHEDULE PROPOSED SCHEDULE Jan. TASK 1 Literature Review TASK 2 Methodology Development Subtasks 2.1 Methodology Development 2.2 Science Team Presentation TASK 3 Subtasks Feb. March April May 2015 June July Aug Sept Oct Applied Research 3.1 FAB laboratory studies* 3.2 Data Reduction and analysis TASK 4 Draft and Final Reports TASK 5 Meetings and Presentations * Three living streams and two raceways at FAB needed from March through July BIO-WEST REFERENCES In addition to the eleven Applied Research projects directly related to the endangered species of the Comal and San Marcos ecosystems highlighted above, the following references are included for the Authority’s review. Attachments B1 – B3 38 Nov Dec BIO-WEST Riffle Beetle Habitat Connectivity Study ATTACHMENT B1 CLIENT REFERENCE For each reference, complete the following information: Client Name: San Antonio River Authority Client Contact Name: Steve Raabe Position: Chief Technical Officer Client Address: San Antonio River Authority 100 E. Guenther Road San Antonio, TX 78283 Client Telephone Number(s): (210) 302-3614 Date Contract Began: July 2006 through present Description of Services: BIO-WEST was selected as the prime contractor to assist the San Antonio River Authority with a multi-faceted insstream flow study on the San Antonio River and Cibolo Creek. The overall study was completed in 2011 for approximately $1.2 million. The 2014 contract is for continued long-term monitoring of the aquatic and riparian communities. A key component of the monitoring is an Applied Research effort focused on riparian habitat at two intensive study sites located on the lower San Antonio River. Cost: $225,000 / year B-1 39 BIO-WEST Riffle Beetle Habitat Connectivity Study ATTACHMENT B2 CLIENT REFERENCE For each reference, complete the following information: Client Name: Southern Nevada Water Authority Client Contact Name: Position: Zane Marshall Director of the Water & Environmental Resources Department Client Address: Southern Nevada Water Authority 100 City Parkway, Suite 700 Las Vegas, Nevada 89193 Client Telephone Number(s): (702) 822-3351 Date Contract Began: January 2004 through present Description of Services: BIO-WEST evaluated existing information and facilitated a Biological Working Group (BWG) consisting of four U.S. Department of the Interior bureaus: the Bureau of Indian Affairs, the Bureau of Land Management, USFWS, and the National Park Service; and the Nevada Department of Wildlife. BIO-WEST developed a consistent springs’ characterization methodology and conducted a comprehensive baseline sampling effort in Nevada’s Clark, Lincoln, and White Pine counties. The information collected is being used to provide an assessment of the changes that may potentially result from the implementation of the counties’ groundwater project. The biological sampling effort includes an assessment of water quality, aquatic vegetation, fish and aquatic invertebrates, non-native species, threatened and endangered species, and species of concern. A major component of BIO-WEST’s involvement was to develop and conduct Applied Research studies to 1) support evaluation and refinement of the Monitoring Plan, and 2) to support GWD Project environmental planning / compliance, as needed. These activities are being pursued to meet SNWA commitments and planning needs under the Spring Valley Stipulation, Nevada State Engineer rulings, and NEPA and ESA compliance. Cost: $300,000 B2 40 BIO-WEST Riffle Beetle Habitat Connectivity Study ATTACHMENT B3 CLIENT REFERENCE For each reference, complete the following information: Client Name: Lower Colorado River Authority Client Contact Name: Position: Bryan Cook Senior Fisheries Biologist Client Address: Lower Colorado River Authority 3700 Lake Austin Blvd. Austin, TX 78703 Client Telephone Number(s): (512) 473-3551 Date Contract Began: July 1, 2004 – continued assessments Radio-tagged blue sucker recaptured 16 months after tagging. Description of Services: BIO-WEST was contracted by the Lower Colorado River Authority and completed a complex multi-year environmental analysis of instream-flow conditions and requirements of the biological community in the Colorado River. BIO-WEST collected extensive biological and physical habitat data to model and predict the potential effects of changes in flow patterns on fishes, aquatic invertebrates and the riparian community. The second major component of the study was an Applied Research effort that involved tagging and monitoring the habitat usage and migration patterns of the blue sucker over a three year period. Cost: $1,350,000 B-3 41 BIO-WEST Riffle Beetle Habitat Connectivity Study RESUMES – To save resources – resumes are provided in digital format for all copies. 42 BIO-WEST, Inc. James Randall Gibson, Senior Macroinvertebrate Biologist Mr. Gibson specializes in aquatic macroinvertebrates, and research and EXPERIENCE maintenance of captive populations of threatened and endangered species ► Aquatic macroinvertebrates including the Comal Springs riffle beetle (Heterelmis comalensis), Peck’s cave amphipod (Stygobromus pecki), Devils River minnow (Dionda diaboli), ► Threatened and Endangered fountain darter (Etheostoma fonticola), San Marcos salamander (Eurycea nana), Species Texas blind salamander (Typhlomolge rathbuni), and Texas wildrice (Zizania texana). He has 14 years of professional experience working as both a fish ► Applied Research biologist for the U.S. Fish and Wildlife Service, Aquatic Resource Center, San Marcos, Texas and as a Senior Macroinvertebrate Specialist for BIOWEST. Mr. Gibson’s professional duties include: construction, SKILLS maintenance, and repair of systems used for refugia and research; ► Refugia development designing, conducting, and writing reports on scientific experiments with these species (culture and reproduction of captive Devils River minnows and survival, reproduction, and development of Comal Spring riffle beetle ► Experimental design in captivity); and performing field work primarily in the San Marcos and ► Field investigations Comal Rivers involving ecological surveys, water quality measurements, effect and abundance of parasites and exotic species, cataloging progress of ► Identification protected species in the wild; and collection of threatened and endangered species for research and the refugia. Mr. Gibson received an MS in Zoology and B.S in Biology from Texas A&M University. EDUCATION A brief summary of Mr. Gibson’s relevant BIO-WEST experience is presented below. ► BIO-WEST PROJECT WORK ► Edwards Aquifer Bio-Monitoring, Comal and San Marcos Rivers ♦ Senior Macroinvertebrate Biologist This BIO-WEST project entails a multidiscipline, multiyear variable flow and water-quality study for the Edwards Aquifer Authority (EAA). This program has been incorporated into the long-term Bio-Monitoring associated with the Edwards Aquifer Recovery Implementation Program (EARIP) Habitat Conservation Plan (HCP). BIO-WEST is conducting applied research and monitoring of several threatened and endangered species in the spring-fed Comal and San Marcos Rivers in Texas. The HCP Bio-Monitoring incorporates comprehensive sampling and flow-dependent sampling that is conducted when the discharge in one or both of the rivers falls below (low-flow) or rises above (high-flow) specified “trigger” levels. Under contract with EAA. Mr. Gibson is in charge of all endangered macroinvertebrate sampling involving cotton lures and drift netting over spring orifices from.2001–present. 1 2000: MS Zoology, Texas A&M University, College Station 1990: BS Biology, Texas A&M University, College Station BIO-WEST PROJECT WORK (cont.) Applied Research – Edwards Aquifer Endangered Species ♦ Senior Macroinvertebrate Biologist Mr. Gibson is providing guidance and technical support for the Low-Flow Food Source Applied Research Study being contracted in 2013 to inform the development of the Edwards Aquifer Habitat Conservation Plan Ecological Model. The objective of this study was to use native Hyalella azteca from the Comal system as a surrogate to evaluate “What will happen to invertebrate food sources for the fountain darter under extreme low-flow conditions?” Under contract with the Edwards Aquifer Authority. 2013–present. Comal Springs Riffle Beetle Habitat and Population Evaluation ♦ Co-Principal Investigator BIO-WEST conducted an intensive search effort for the endangered Comal Springs riffle beetle (Heterelmis comalensis) and documented an extension of the known range of species in Comal Springs. Mr. Gibson participated in field efforts that searched spring habitats along the Landa Lake shoreline (along spring-run habitat) and in several locations where springs were evident within the lake to find populations of species outside of the known range. Mr. Gibson performed quantitative measurements to examine the relative densities of the Comal Springs riffle beetle and a similar riffle beetle species where populations of the former were found. The range extension of this species was developed into a manuscript for submission to a peer-reviewed journal. Under contract with EAA. 2001. Comal Springs Riffle Beetle Laboratory Studies ♦ Co-Principal Investigator After the Comal Springs riffle beetle habitat and population evaluation, BIO-WEST raised questions about responses to changes in springflow, both laterally and vertically, and studied concerns regarding changes in the laboratory. Mr. Gibson was Co-Principal Investigator on laboratory studies and participated in project development including narrowing study foci to address specific questions and assisting with designing the laboratory setup. In this laboratory study, Comal Springs riffle beetles displayed a tendency for downward movement through the substrate and a preference to be in, and move toward moving water (current). When horizontal flow was applied to the block, the beetles were most commonly found towards the front. When a vertical upwelling flow was applied to the bottom, most beetles were collected near or moved toward the bottom. Therefore, it is feasible that these beetles would respond to decreased spring flows by moving downward into the substrate in search of a flow stimulus. It is also likely that these beetles inhabit areas deeper in the gravel and sediment than have previously been sampled. Under contract with EAA. 2002. PUBLICATIONS BIO-WEST. 2013. Comprehensive and critical period monitoring program to evaluate the effects of variable flow on biological resources in the Comal Springs/River aquatic ecosystem. 2001–2012 Annual Reports. Prepared for Edwards Aquifer Authority. Jean A., N. D. Telles, J. R. Gibson, D. Foley and K. B. Miller. 2012. Description of a New Genus and Species of Stygobiontic Diving Beetle, Psychopomporus felipi Jean, Telles, and Miller (Coleoptera: Dytiscidae: Hydroporinae), from the Edwards-Trinity Aquifer System of Texas, USA. Phillips, C. T., J. R. Gibson, and J. N. Fries. 2011. Spawning behavior and nest association by Dionda diabolic in the Devils River, Texas. Southwestern Naturalist 56:108-112. 2 PUBLICATIONS (cont.) Kulkoyluoglu, O., R. Gibson, P. H. Diaz, and Jean-Paul Colin. 2011. Bicornucandona gen. nov.,sp. nov. (Crustacea, Ostracoda) from Finegan Spring (Texas, U.S.A.). Zootaxa 3059:47-58. Carson, E. W., A. H. Hanna, G. P. Garrett, J. R. Gibson, and J.R. Gold. 2010. Conservation genetics of Cyprinid fishes (Genus Dionda) in southwestern North America. II. Expansion of the known range of the Manantial roundnose minnow, Dionda argentosa. Southwestern Naturalists 55:576-581. Fries, J. N., and J.R. Gibson. 2010. Critical thermal maxina of captive-bred Devils River minnows (Dionda diabolic). Southwestern Naturalist 55:544-550. Miller, K. B., J. R. Gibson, and Y. Alarie. 2009. North American Stygobiontic diving beetles (Coleoptera: Dytiscidae: Hydroporinae) with description of Ereboporus naturaconservatus Miller, Gibson and Alarie, new genus and species, from Texas, U.S.A. The Coleopterists Bulletin 63(2):191-202. Phillips, C. T., J. R. Gibson, and J. N. Fries. 2009. Agonistic and Courtship Behavior in Dionda diaboli, the Devils River Minnow. The Southwestern Naturalist 54(3):341-368. Gibson J. R., S. J. Harden, and J. N. Fries. 2008. Survey and Distribution of Invertebrates from Selected Springs of the Edwards Aquifer in Comal and Hays Counties, Texas. The Southwestern Naturalist 53(1):74-84. Gibson, J. R. and J. N. Fries. 2005. Culture Studies of the Devils River Minnow. North American Journal of Aquaculture 67:294-303. Gibson, J. R., J. N. Fries, G.P. Garrett. 2004. Habitat and Substrate Use in Reproduction of Captive Devils River Minnow. North American Journal of Aquaculture 66:42-47. BIO-WEST. 2002. Comal Springs riffle beetle habitat and population evaluation. Prepared for Edwards Aquifer Authority. BIO-WEST. 2002. Comal Springs riffle beetle laboratory study: evaluation under variable flow conditions. Final report prepared for Edwards Aquifer Authority, San Antonio, Texas. 3 BIO-WEST, Inc. Edmund Oborny, Senior Fisheries Biologist Mr. Oborny specializes in aquatic ecology, threatened and endangered species, water quality, biological modeling, and instream flow issues and concepts. He has 19 years of professional project experience, and is familiar with all levels of project management and complex study design. Mr. Oborny possesses technical expertise in fisheries biology, instream and environmental flow, water quality, ichthyology, aquatic ecology, and modeling. He has worked on many projects with endangered species components in Texas and the southwestern United States; prepared Environmental Impact Statements and other environmental documents; and participated in freshwater and coastal monitoring programs, waterquality investigations, instream flow analyses, radio-telemetry studies, and numerous other fishery- and water-related projects. Mr. Oborny has also managed several large ecological- and water-resource projects; taken courses in two-dimensional modeling with SMS, instream flow incremental methodology (IFIM), physical-habitat simulation modeling, water-surface profiling and floodplain analysis, and applied river geomorphology; and used indicators of hydrologic alteration and range of variability approach to assess ecological impacts associated with groundwater withdrawal on the Comal and San Marcos Springs aquatic ecosystems. In addition, he has used habitat suitability indices (HSI) and hydraulic modeling to evaluate impacts/benefits to the aquatic habitat of riverine species/life stages and HSI and habitat evaluation procedures to evaluate the impacts/benefits to estuarine species with respect to changes in salinity. Mr. Oborny received an MS in wildlife and fisheries sciences from Texas A&M University and a BS in wildlife biology from Eastern New Mexico University. A brief summary of Mr. Oborny’s relevant experience is presented below. EXPERIENCE ► instream flow analysis ► project management ► study design ► SKILLS ► ► ► ► ► ► This BIO-WEST project entails a multidiscipline, multiyear variable flow and water-quality study for the Edwards Aquifer Authority (EAA). This program has been incorporated into the long-term Bio-Monitoring associated with the Edwards Aquifer Recovery Implementation Program (EARIP) Habitat Conservation Plan (HCP). BIO-WEST is conducting applied research and monitoring of several threatened and endangered species in the spring-fed Comal and San Marcos Rivers in Texas. The HCP Bio-Monitoring incorporates comprehensive sampling and flow-dependent sampling that is conducted when the discharge in one or both of the rivers falls below (low-flow) or rises above (high-flow) specified “trigger” levels. The overall study objectives are to (1) track habitat usage and population dynamics over time, (2) evaluate effects to habitat and species during drought and following flooding, and (3) to evaluate the effectiveness of 1 two-dimensional hydraulic modeling instream flow habitat modeling (PHABSIM, MesoHABSIM, and HSI) PROFESSIONAL SOCIETIES BIO-WEST PROJECT WORK Edwards Aquifer Bio-Monitoring, Comal and San Marcos Rivers ♦ Project Manager/Principal Aquatic Resources Investigator freshwater and coastal monitoring 2003–2013: Desert Fishes Council 2000–2010: Texas Rivers and Reservoirs Management Society 2000–2013: American Fisheries Society 2007–2010: Ecological Society of America EDUCATION ► ► 1993: MS wildlife and fisheries sciences (fisheries), Texas A&M University, College Station 1991: BS wildlife biology, Eastern New Mexico University, Portales BIO-WEST PROJECT WORK (cont.) implemented HCP measures. Under contract with EAA. Mr. Oborny manages and informs all aspects of this project. 2001–present. Applied Research – Edwards Aquifer Endangered Species ♦ Project Manager/Principal Investigator Mr. Oborny is project manager and Co-Principal Investigator on four Applied Research Studies being contracted in 2013 to inform the development of the Edwards Aquifer Habitat Conservation Plan Ecological Model: • Laboratory verses Field - the objective of this study was to compare growth of aquatic vegetation in the field versus the laboratory while grown under similar environmental conditions. • Vegetation Tolerance Trials - Objectives of this study were to evaluate the effects of low-flow conditions on aquatic vegetation survival and growth. • Bicarbonate Utilization Potential - pH Drift Study - Objectives of this study were to determine which aquatic plant species are capable of HCO3 utilization. • Low-flow Food Source Study - The objective of this study was to use native Hyalella azteca from the Comal system as a surrogate to evaluate “What will happen to invertebrate food sources for the fountain darter under extreme low-flow conditions?” Under contract with the Edwards Aquifer Authority. 2013–present. Ecological Modeling – Edwards Aquifer Habitat Conservation Plan ♦ Project Principal Mr. Oborny is project principal and is working with scientists from the University of Texas, Texas A&M University, Baylor University, Texas State University, and the USACE Environmental Laboratory to develop an ecological model for the endangered species of the Comal and San Marcos Springs ecosystems. The objective of the predictive ecological model for the Comal and San Marcos ecosystem project is to adapt existing models for aquatic vegetation and fountain darters and begin the development of models for Texas wild-rice and gill parasites impacts. Under contract with the Edwards Aquifer Authority. 2013–present. San Antonio River Authority Instream Flow Planning Project ♦ Project Manager/Lead Biologist Mr. Oborny is participating in instream flow planning activities at the San Antonio River Basin in Texas. Project activities have included completing an instream flow evaluation of the Salatrillo/Martinez Creeks watershed in northern Bexar County and completing a detailed instream flow assessment of the Lower San Antonio River in conjunction with the Texas Instream Flow Program (TIFP). Presently, BIO-WEST is working with the San Antonio River Authority on follow-up monitoring and applied research investigations involving seasonal use of habitat by indicator fish species and riparian zone research to examine the effects of lower San Antonio River pulse flows. Under contract with the San Antonio River Authority. 2006– present. Lower Guadalupe River Instream Flow Study, Guadalupe River ♦ Project Manager/ Principal Investigator Mr. Oborny is leading a full-scale instream flow study for the Gonzales reach of the lower Guadalupe River in conjunction with Guadalupe-Blanco River Authority staff and interaction with the TIFP. The study objective is to characterize the flow-habitat and flow-ecological relationships in this reach to provide a means of assessing biological impacts/benefits of various flow regimes relative to the Mid-Basin and future projects. A comprehensive ecologically based tool generated from existing studies and field-gathered data 2 BIO-WEST PROJECT WORK (cont.) will provide prediction capabilities necessary to evaluate a full flow regime on ecological components of the lower Guadalupe River within this reach. Under contract with the Guadalupe-Blanco River Authority. 2012–present. Blue Sucker Tracking Study, Lower Sabine River ♦ Project Principal Mr. Oborny is project principal for a radio-telemetry study of the blue sucker (Cycleptus elongatus), which is listed as a state-threatened species. BIO-WEST is working with Texas Parks and Wildlife Department (TPWD) fisheries biologists to characterize seasonal movements and habitat use of the blue sucker in the lower Sabine River through radio telemetry. The primary objectives of the study are to determine spawning locations by locating sexually mature adults, track movements during different seasons, and determine differential habitat use among varying life stages. Under contract with TPWD. 2012–present. Native Aquatic Vegetation Restoration, Comal Springs ♦ Project Principal/Principal Investigator The EARIP process led to the development of the approved HCP for the Comal Springs and Comal River system (Comal system) in New Braunfels, Texas. In relation to the Comal system, the EARIP process and HCP identify a variety of options to improve and increase habitat for Federally listed threatened and endangered species. Native aquatic vegetation restoration is a critical HCP measure being implemented in 2013. Mr. Oborny is leading a project team with the overall goal to improve habitat conditions for the fountain darter (Etheostoma fonticola) in Landa Lake and the Old Channel. This goal will be achieved by increasing the amount of usable habitat and improving the quality of existing habitat in both project areas. Under contract with City of New Braunfels. 2013–present. Gill Parasite Evaluation, Comal Springs ♦ Project Principal As part of the EARIP HCP, a comprehensive gill parasite evaluation of the Comal River is being conducted by BIO-WEST. Mr. Oborny is project principal overseeing work that includes a system-wide survey to determine Melanoides tuberculatus (nonnative host snail) population densities and cercarial concentrations of Centrocestus formosanus (gill parasite). Additionally, methods for the reduction of the gill parasite in the Comal system are being tested for effectiveness and efficiency. Finally, a gill parasite monitoring and reduction program (if necessary) will be developed for implementation in subsequent years. Under contract with City of New Braunfels. 2013–present. Gill Parasite Evaluation Pilot Study ♦ Project Manager BIO-WEST conducted a pilot study to evaluate the effectiveness of host snail removal on the reduction of Centrocestus formosanus (gill parasite) in the Comal Springs system, New Braunfels, Texas. Mr. Oborny oversaw all aspects of project work. Under contract with the EARIP. 2010–2011. Trinity River Instream Flow Assistance, Trinity River ♦ Project Manager/Principal Investigator Mr. Oborny is providing technical guidance to the Trinity River Authority (TRA) regarding instream flow activities they are conducting on the middle Trinity River. In conjunction with TRA staff and interaction with the TIFP, the study objective is to characterize the flow-habitat and flow-ecological relationships in this reach of the river to provide a means of assessing biological impacts/benefits of various flow regimes relative to existing conditions and future projects. Under contract with the TRA. 2012–present. 3 BIO-WEST PROJECT WORK (cont.) Brazos River Instream Flow Assistance, Brazos River Basin ♦ Project Manager/Principal Investigator Mr. Oborny is project principal for instream flow planning and field activities associated with the Brazos River Authority Water Management Plan. This includes activities on the main stem and tributaries of the Brazos River throughout the basin and on streams throughout the Little River watershed. In conjunction with Brazos River Authority staff, BIO-WEST is conducting fish, macroinvertebrate, mussel, and riparian investigations in a manner consistent with TIFP activities. Under contract with the RPS Espey Consultants. 2011–present. Lower Colorado River Authority Unappropriated Flows Permit ♦ Project Manager BIO-WEST provided technical assistance for the Lower Colorado River Authority’s (LCRA) unappropriated flows permit application. The permit applied to waters in Austin, Texas. Under contract with Espey Consultants, Inc. 2008–2010. Owens Lake Groundwater Evaluation ♦ Project Manager Under an agreement with the Los Angeles Department of Water and Power, Mr. Oborny is providing technical oversight as part of a blue ribbon science panel relative to the Owens Lake Groundwater Project. The purpose of the project is to evaluate the feasibility of plans to supply groundwater for dust control measures by analyzing hydrology, hydrogeology, geology, existing natural resources that depend on groundwater, possible impacts from groundwater withdrawals on these resources, and proposed methods to avoid or mitigate such impacts. Under contract with MWH Americas, Inc. 2009–present. Lower Sabine River Fish Study ♦ Project Manager BIO-WEST provided fisheries consulting services to the Sabine River Authorities of Texas and Louisiana in connection with the Toledo Bend Relicensing Project. The project area consisted of the Sabine River, which forms the boundary between the states of Texas and Louisiana, from Toledo Bend Dam to River Mile 64 downstream. The study objective was to provide a comprehensive review of the existing fisheries environment of the lower Sabine River downstream of Toledo Bend Dam and evaluate potential project operational influence on that downstream environment. Under contract with HDR/DTA. 2009–2011. Eastern Sierra Streams Peer Review ♦ Project Manager Mr. Oborny conducted a third-party review of fisheries, benthic macroinvertebrate, and instream flow data and reports dealing with Eastern Sierra stream conditions and management as well as provided an independent review and prepared written documentation for and critiques of materials provided by the client. The documentation summarized the results and rationale of BIO-WEST’s review of data and reports. Specifically, Mr. Oborny provided comments on the recommendations for ongoing stream maintenance and restoration efforts proposed by others. Under contract with MWH Americas, Inc. 2009–2010. Texas Department of Transportation Spur 53 Highway Expansion ♦ Project Manager BIO-WEST provided an analysis of stream modifications and associated habitats for the Spur 53 highway expansion project for the Texas Department of Transportation in San Antonio, Texas. Under contract with Zara Environmental, LLC. 2010. 4 BIO-WEST PROJECT WORK (cont.) BBASC Technical Support ♦ Project Manager The Guadalupe, San Antonio, Mission, and Aransas Rivers and Mission, Copano, Aransas, and San Antonio Bays Basin and Bay Area Stakeholders Committee (GSA BBASC), in fulfilling its charge under the Texas Water Code, hired BIO-WEST to provide technical support to assist with evaluating recommendations of the Basin and Bay Expert Science Team (GSA BBEST). Under contract with HDR Engineering, Inc. 2011. Colorado River Flow Relationships to Aquatic Habitat and State-Threatened Blue Sucker Species ♦ Project Manager Mr. Oborny was the project manager for an instream flow study that (1) developed hydraulic modeling tools and (2) determined habitat-use criteria for fish in the lower Colorado River, Texas, to assess potential changes in habitat availability under various water-release strategies. The study involved sampling all life stages of the state-threatened blue sucker including telemetry tracking of 30 tagged adults. Mr. Oborny supervised all aspects of project work including meetings, presentations, data collection, hydraulic and habitat modeling, sediment-transport and riparian analyses, and dissemination of results; oversaw model evaluation and interpretation; and was instrumental in the development of instream flow criteria for the lower Colorado River. Under contract with the LCRA. 2004–2008. Matagorda Bay Health Evaluation ♦ Project Manager/Senior Fisheries Biologist Mr. Oborny provided managerial and biological services for the habitat component of the Matagorda Bay Health Evaluation (MBHE) that was conducted as part of the LCRA/San Antonio Water System (SAWS) water project. The habitat component of the MBHE provided an assessment of existing habitat conditions within the project area and allowed for the comparison of habitat conditions between different freshwater, inflow scenarios through hydrodynamic salinity modeling. The habitat assessment generated a model in GIS format based on a series of relationships between biota and the physical and chemical environment of Matagorda Bay. Output from the habitat model was provided both in tabular form (detailing the aerial extent of output) and in spatial form (with the potential to be integrated or correlated with other spatial information and analyzed based on location of interest). Under contract with LCRA. 2004–2008. Springs Ecological Evaluation and Mapping of Biological Resources, Nevada and Utah ♦ Project Principal BIO-WEST conducted a comprehensive springs ecological evaluation of the Great Basin that focuses on unique ecosystems and endemic species including seven Federally threatened or endangered species, two Conservation Agreement species, a host of Federal species of concern, and state-sensitive species. BIOWEST used a variety of criteria to select and evaluate more than 100 systems of interest throughout a geographical region where the Southern Nevada Water Authority (SNWA) proposed to develop groundwater resources. Biological efforts focused on documenting and inventorying species present in the unique spring system environment of east-central Nevada and west-central Utah. Ecological surveys expanded knowledge of aquatic and riparian vegetation, fish, amphibians, and macroinvertebrates and identified potential disturbance factors, as well as restoration opportunities. BIO-WEST’s surveys also expanded the range of several species, may have identified two new species of macroinvertebrates, and uncovered a population of dace (Leuciscus spp.) that was thought to have been extirpated by the late 1990s. Mr. Oborny was tasked with overseeing the project. Under contract with SNWA. 2004–2011. 5 BIO-WEST PROJECT WORK (cont.) Waterfowl/Wildlife Comprehensive Study and Long-Term Monitoring ♦ Project Principal BIO-WEST participated in a comprehensive assessment of potential impacts/benefits to waterfowl and wildlife in south-central Texas for the LCRA/SAWS water supply project (WSP). A regional concern was raised regarding potential impacts to the local economies of Colorado, Wharton, and Matagorda Counties from wildlife (primarily waterfowl) changes associated with the WSP. Anticipated decreases in rice production—with or without the WSP—would likely have had an impact on wildlife including waterfowl populations, such as ducks and geese. BIO-WEST evaluated associated impacts/benefits to waterfowl populations and developed alternatives to the WSP. BIO-WEST’s evaluation was accomplished by (1) establishing existing conditions in respect to land use and wildlife utilization, (2) determining socioeconomic contributions related to waterfowl and wildlife, (3) evaluating proposed off-channel storage facilities, and (4) assessing WSP proposed agricultural strategies. Mr. Oborny served as project principal and oversaw the project. Under contract with LCRA. 2007–2009. Bernalillo to Alameda Bridge River Restoration ♦ Senior Fisheries Biologist BIO-WEST was contracted to provide technical expertise to the U.S. Bureau of Reclamation (Reclamation) project team on several components of a multifaceted restoration effort on 10 miles of the middle Rio Grande in New Mexico. Mr. Oborny’s responsibilities included assembling and presenting existing southwestern willow flycatcher (Empidonax traillii extimus) and Rio Grande silvery minnow (Hybognathus amarus) data, evaluating methods for creating a two-dimensional model of existing conditions, reviewing existing information on endangered species, and presenting potential benefits of creating a two-dimensional model for quantifying hydraulic conditions and endangered species habitat at selected index sites. Under contract with Reclamation. 2003–2005. Provo River Flow Study ♦ Assistant Project Manager/Principal Aquatic Resources Investigator This BIO-WEST study was designed to determine the effects of alternative flow regimes to the middle and lower sections of the Provo River, Utah, and the remaining portions of its riparian corridor. The project area encompassed approximately 30 miles of the Provo River and its riparian ecosystem from Jordanelle Dam to Utah Lake. The objective of the project was to collect field data and develop modeling capabilities for use in the characterization of flow-channel processes and flow-ecological relationships (within the Provo River and its riverine ecosystem). The results of this study provided modeling and prediction capabilities necessary to evaluate the effects of alternative flow regimes on ecological components (including the endangered June sucker [Chasmistes liorus]) throughout the annual hydrologic cycle and helped predict how aquatic habitat and recruitment of riparian vegetation would likely change over time. Mr. Oborny assisted with managing and informing all project-related activities. Under contract with the Utah Reclamation Mitigation and Conservation Commission. 2002–2004. Comal Springs Riffle Beetle Habitat and Population Evaluation ♦ Principal Investigator/ Project Manager BIO-WEST conducted an intensive search effort for the endangered Comal Springs riffle beetle (Heterelmis comalensis) and documented an extension of the known range of species in Comal Springs. Mr. Oborny coordinated field crews of two to three biologists who searched spring habitats along the Landa Lake shoreline (along spring-run habitat) and in several locations where springs were evident within the lake to find populations of species outside of the known range. Mr. Oborny performed quantitative measurements to examine the relative densities of the Comal Springs riffle beetle and a similar riffle beetle species where 6 BIO-WEST PROJECT WORK (cont.) populations of the former were found. The range extension of this species was developed into a manuscript for submission to a peer-reviewed journal. Under contract with EAA. 2001. Comal Springs Riffle Beetle and Fountain Darter Laboratory Studies ♦ Principal Investigator/ Project Manager After the Comal Springs riffle beetle habitat and population evaluation, BIO-WEST raised questions about responses to changes in springflow, both laterally and vertically, and studied concerns regarding changes in the laboratory. Mr. Oborny managed laboratory studies and participated in project development including narrowing study foci to address specific questions and assisting with designing the laboratory setup. BIOWEST also raised questions about the response of fountain darter reproduction to diel fluctuations in temperature that are observed in the wild, but not addressed in previous laboratory studies. Mr. Oborny worked closely with Dr. Tim Bonner of Southwest Texas State University, who was instrumental in earlier studies, and other BIO-WEST personnel to develop the project, analyze results, and prepare a detailed executive summary. Under contract with EAA. 2002. Spring Lake Dam Reconstruction Effort ♦ Principal Investigator/Project Manager BIO-WEST performed monitoring and manual displacement of threatened and endangered species that were subject to potential impacts caused by reconstruction activities on a dam impounding Spring Lake on the San Marcos River. Monitoring included surveying the endangered fountain darter and threatened San Marcos salamander (Eurycea nana) and mapping Texas wild-rice (Zizania texana) populations. Fountain darter and San Marcos salamander populations were displaced immediately prior to power-washing and rip-rap placement. Periodic spot-checks and reconstruction monitoring efforts were conducted to ensure that efforts were being made to limit impacts on these populations. Mr. Oborny was responsible for overall project management. Under contract with Texas State University. 2001–2002. Lake Sam Rayburn Aquatic Surveys ♦ Project Principal This large-scale effort entailed providing technical assistance to a client who needed to gain understanding of water, sediment, and fish-tissue chemistry; water and sediment toxicity; fish, wildlife, and aquatic-habitat conditions; and implications of studies and proposed actions related to operations at a paper mill in east Texas. Mr. Oborny conducted field sampling; analyzed water and sediment chemistry, water and sediment toxicity, and fish and macroinvertebrate communities using the Texas Commission on Environmental Quality methodology for Receiving Water Assessments and Use Attainability Analysis; and designed the comprehensive sampling effort to evaluate fish and habitat conditions in the Angelina River, Sam Rayburn Reservoir, and surrounding watersheds. Under contract with the Conservation Coalition. 2002–2005. Texas Department of Transportation Mussel Surveys ♦ Project Manager BIO-WEST conducted Phase 1 habitat surveys for rare and state-listed freshwater mussel species and additional aquatic species at eight road crossings of perennial waters within the Texas Department of Transportation San Antonio Region. The surveys included the area from 100 meters upstream of bridges associated with waterway crossings to 300 meters downstream of the bridges. Mr. Oborny directed the project. Under contract with TRC Environmental Corporation. 2010. 7 BIO-WEST PROJECT WORK (cont.) Sabine Lake Marsh Sampling and Salinity Characterization ♦ Project Manager BIO-WEST provided technical services to assist the National Wildlife Federation (NWF) with an evaluation of salinity conditions and marsh vegetation within and adjacent to Sabine Lake in Texas. The study was designed to document salinity conditions and resulting marsh vegetation types spatially within the complex marshes on the eastern side of Sabine Lake. An evaluation of salinity changes and gradients under differing freshwater inflow conditions was also conducted. Data collected during this effort provided a solid baseline to build on should additional resources become available and data on marsh biomass and/or juvenile organism utilization of these habitats be desired. Under contract with the NWF. Mr. Oborny provided project oversight and direction. 2010–2011. OFFICES AND APPOINTMENTS Texas Environmental Flows Science Advisory Committee ♦ Advisory Committee Member Serves on the Texas Environmental Flows Science Advisory Committee, which is appointed by the Texas Environmental Flows Advisory Group of the Texas State Legislature. Responsible for providing objective, scientific advice on issues relating to the science of environmental flow protection, as well as developing recommendations that will ensure consistent use of flow methodologies and environmental flow programs. 2009–present. Third-Party Independent Review of Mono Basin Stream Ecosystem Flows Recommendations ♦ Instream Flows Science Expert Mr. Oborny was selected, along with three other nationally recognized instream flow experts, to conduct an independent, third-party review of 12 years of fisheries, geomorphology, and riparian monitoring conducted in the Mono Basin, California, for the establishment of instream flow recommendations. 2009. Edwards Aquifer Recovery Implementation Program ♦ Science Subcommittee Member Appointed by the EARIP Steering Committee to be a voting member of the Science Subcommittee, which is responsible for addressing science-based questions/charges set forth by the Texas Legislature. Charges focus on the determination of flow requirements for Federally listed endangered and threatened species of the Edwards Aquifer and Comal and San Marcos Springs. 2008–2011. Southern Edwards Aquifer Species Recovery Team ♦ Team Member Appointed by the Regional Director of the U.S. Fish and Wildlife Service (USFWS) to be part of the Southern Edwards Aquifer Species Recovery Team. The objective of the team is to update and revise a Recovery Plan for seven Federally listed endangered species and one Federally listed threatened species in the Comal and San Marcos Springs ecosystems and in Edwards Aquifer. 2008–present. The Biological Working Group of Spring Valley, Nevada ♦ Special Consultant Retained to assist with (1) developing a multiyear, multifaceted monitoring plan to further the understanding of groundwater-influenced ecosystem dynamics and (2) tracking biotic-community responses to SNWA’s groundwater withdrawal from the Spring Valley Hydrographic Basin in east-central Nevada. The monitoring plan was the result of a stipulated agreement between SNWA and four U.S. Department of the Interior bureaus: the Bureau of Indian Affairs, the Bureau of Land Management, USFWS, and the National Park Service. 2007–present. 8 OFFICES AND APPOINTMENTS (cont.) The Blue Ribbon Science Advisory Panel of Owens Valley, California ♦ Panel Member (Aquatic Resources) Appointed to the Blue Ribbon Science Advisory Panel to analyze hydrology, hydrogeology, geology, and existing natural resources that depend on groundwater within Owens Valley, California, relative to proposed projects. The panel will perform an assessment of possible impacts from groundwater withdrawals on resources and evaluate proposed methods to avoid or mitigate impacts. 2009–present. PUBLICATIONS / PRESENTATIONS Johnson, M.S., A. Bolick, M. Alexander, D. Huffman, E.L. Oborny, and A. Monroe. 2012. Fluctuations in densities of the invasive parasite Centrocestus formosanus (Trematoda: Heterophyidae) in the Comal River, Comal County, Texas, U.S.A. Journal of Parasitology 98(1):111–116. McDonald, D.L., T.H. Bonner, E.L. Oborny, and T.M. Brandt. 2007. Effects of fluctuating temperatures and gill parasites on reproduction of the fountain darter, Etheostoma fonticola. Journal of Freshwater Ecology 22(2):311–318. Oborny, E.L., et al. [BIO-WEST]. 2013. Comprehensive and critical period monitoring program to evaluate the effects of variable flow on biological resources in the Comal Springs/River aquatic ecosystem. 2001–2012 Annual Reports. Prepared for Edwards Aquifer Authority. Oborny, E.L., et al. [BIO-WEST]. 2013. Comprehensive and critical period monitoring program to evaluate the effects of variable flow on biological resources in the San Marcos Springs/River aquatic ecosystem. 2001–2012 Annual Reports. Prepared for Edwards Aquifer Authority. Oborny, E.L., et al. [BIO-WEST]. 2011. Instream flow study of the lower San Antonio River and lower Cibolo Creek. interim progress report and instream flow recommendations. Texas Instream Flow Program and San Antonio River Authority. Oborny, E.L., et al. [BIO-WEST]. 2008. Colorado River flow relationships to aquatic habitat and state threatened species: blue sucker - instream flow guidelines. Prepared for the Lower Colorado River Authority and San Antonio Water System. Oborny, E.L., et al. [Matagorda Bay Health Evaluation Scientists]. 2008. Matagorda Bay inflow criteria. Matagorada Bay health evaluation: final report. Prepared for the Lower Colorado River Authority and San Antonio Water System. Oborny, E.L., et al. [BIO-WEST]. 2008. Preliminary instream flow assessment for the Lower San Antonio River (interim subsistence and base-dry instream flow guidelines development). Prepared for the San Antonio River Authority. Oborny, E.L., et al. [BIO-WEST]. 2008. Salatrillo and Martinez Creeks instream flow assessment. Prepared for the San Antonio River Authority. Oborny, E.L., et al. [BIO-WEST]. 2007. Variable flow study: seven years of monitoring and applied research. Comprehensive and critical period monitoring program to evaluate the effects of variable flow on biological resources in the Comal Springs/River aquatic ecosystem. Prepared for Edwards Aquifer Authority. PUBLICATIONS / PRESENTATIONS (cont.) Oborny, E.L., et al. [BIO-WEST]. 2006. Colorado River flow relationships to aquatic habitat and state threatened species: blue sucker - 2006 data activities report. Prepared for the Lower Colorado River Authority and San Antonio Water System. Oborny, E.L., et al. [BIO-WEST]. 2006. Matagorda Bay health evaluation habitat assessment: 2006 progress report. Prepared for the Lower Colorado River Authority and San Antonio Water System. Oborny, E.L., et al. [BIO-WEST]. 2005. Colorado River flow relationships to aquatic habitat and state threatened species: blue sucker - 2005 data activities report. Prepared for the Lower Colorado River Authority and San Antonio Water System. Oborny, E.L., et al. [BIO-WEST]. 2005. Matagorda Bay health evaluation habitat assessment: progress report. Prepared for the Lower Colorado River Authority and San Antonio Water System. Oborny, E.L., et al. [BIO-WEST]. 2003. Provo River flow study, Deer Creek Reservoir to Utah Lake: flowhabitat and flow-ecological relationships within the riverine ecosystem - aquatic habitat, riparian vegetation, recreational uses, fluvial processes. Prepared for the Utah Reclamation Mitigation and Conservation Commission, Salt Lake City, Utah. Oborny, E.L., et al. [BIO-WEST]. 2003. Provo River flow study, Jordanelle to Deer Creek: flow-habitat and flow-ecological relationships within the riverine ecosystem - aquatic habitat, riparian vegetation, recreational uses, fluvial processes. Prepared for the Utah Reclamation Mitigation and Conservation Commission, Salt Lake City, Utah. Oborny, E.L., et al. [BIO-WEST]. 2002. Comal Springs riffle beetle habitat and population evaluation. Prepared for Edwards Aquifer Authority. Oborny, E.L., et al. [BIO-WEST]. 2002. Comal Springs riffle beetle laboratory study: evaluation under variable flow conditions. Final report prepared for Edwards Aquifer Authority, San Antonio, Texas. Oborny, E.L., et al. [BIO-WEST]. 2002. Fountain darter laboratory study: reproductive response to parasites and temperature fluctuations. Executive summary prepared for Edwards Aquifer Authority, San Antonio, Texas. Oborny, E.L. 2003. LCRA/SAWS water project specific study plan: Colorado River flow relationships to aquatic habitat. Prepared for the Lower Colorado River Authority. Oborny, E.L. 2003. LCRA/SAWS water project specific study plan - state threatened species: blue sucker. Prepared for the Lower Colorado River Authority. Oborny, E.L. 2001. Environmental and ecological data gaps analysis for future projects along the Lower Colorado River: general overview. Oborny, E.L. 1999. Environmental studies recommendations associated with future projects on the Lower Colorado River. Prepared for the Lower Colorado River Authority, Job. No. 449637. Oborny, E.L. 1997. Evaluation of fish assemblages in the Houston Bayou System. In papers presented at the 14th Annual Gulf Coast Environmental Management Symposium, Pasadena, Texas. 10 PUBLICATIONS / PRESENTATIONS (cont.) Oborny, E.L., and B. Gearhart. 2000. Underwater remote-sensing survey and biological sampling: channel to Smith Point, Galveston Bay, Texas. Prepared for the U.S. Army Corps of Engineers, Galveston District, Document No. 000081. Oborny, E.L., and P. Jensen. 1999. Thermal effects assessment: Bastrop Reservoir. Prepared for GenTex Power Corporation, Document No. 990923. SPECIAL TRAINING AND CERTIFICATION 2009 Stream Temperature Modeling - IF 312, U.S. Geological Survey (USGS) Mid-continent Ecological Science Center 2003 Two-Dimensional Hydraulic Modeling of Complex Waterways with SMS Training 2000 Applied River Geomorphology and Biotechnical Engineering for Fisheries Biologists, American Fisheries Society 1998 Using the Computer Based Physical Habitat Simulation System - IF 310, USGS Midcontinent Ecological Science Center 1997 Theory and Concepts of the IFIM - IF 250, USGS Biological Resources Division 1997 Water Surface Profiling and Floodplain Analysis Seminar featuring HEC-RAS (Haestad Methods) 1996 GIS, Internet, and Fish & Wildlife Microcomputer Applications Course, American Fisheries Society 1994–1999 Mine, Safety, and Health Administration Training Certificate 1989 PADI Open Water SCUBA Diver Certification 11 Weston Hugh Nowlin Texas State University 601 University Drive Department of Biology Aquatic Station San Marcos, TX, USA Office: 512-245-8794 Fax: 512-245-7919 [email protected] http://nowlinaquatecollab.wp.txstate.edu/ ____________________________________________________________________________________________________________ Current Position and Educational History Associate Professor, Department of Biology, Texas State University, San Marcos, Texas September 2011 – Present Assistant Professor, Department of Biology, Texas State University, San Marcos, Texas September 2005 – September 2011 Postdoctoral Research Scholar, Department of Zoology, Miami University, Oxford, Ohio January 2004 – August 2005 Advisor – Michael J. Vanni Ph.D., Biology, University of Victoria, Victoria, British Columbia December 2003 Advisor – Asit Mazumder Master of Science, Biology, Texas Christian University, Fort Worth, Texas December 1998 Advisor – Ray W. Drenner Bachelor of Arts, Biology, Austin College, Sherman, Texas May 1996 Advisor – Peter Schulze Research Interests Ecosystem and Community Ecology Aquatic Ecology (wetlands, lakes, reservoirs) Biogeochemistry Ecotoxicology Weston Hugh Nowlin – Curriculum Vitae 1 Articles in Peer-Reviewed Journals *, †, ‡ and § denote PhD, Masters, undergraduates, and technicians in my lab, respectively Datri, CW†, CL Pray†, Y Zhang, and WH Nowlin. Accepted pending revision (Revisions submitted). Nutrient enrichment exhibits little influence in mediating ecosystem impacts of a non-native herbivore in a spring-fed river. Freshwater Biology. Diaz, PH†, JN Fries, TH Bonner, MA Alexander, and WH Nowlin. Accepted pending revision (Revisions submitted). Mesohabitat associations of the threatened San Marcos salamander (Eurycea nana) across its geographic range. Aquatic Conservation: Marine and Freshwater Ecosystems. Hutchins, BT, BF Schwartz, and WH Nowlin. 2014. Morphological and trophic specialization in a subterranean amphipod assemblage. Freshwater Biology. Early online only. Diaz, PH†, MR Forstner, M Forstner, and WH Nowlin. In press. Survey of aquatic macroinvertebrates in a National Wetland are in Bastrop County, Texas. Texas Journal of Science. Becker, JC*, KJ Rodibaugh†, BJ Labay, TH Bonner, Y Zhang, and WH Nowlin. 2014. Physiographic gradients determine nutrient concentrations more than land use in a Gulf Slope (USA) river system. Freshwater Science. 33:731-744. Brown, DJ, WH Nowlin, E Ozel, I Mali, D Episcopo, MC Jones, MR Forstner. 2013. Comparison of short term low, moderate, and high severity fire impacts to aquatic and terrestrial ecosystem components of a southern USA mixed pine/hardwood forest. Forest Ecology and Management. 312:179-192. Capps, KA, CL Atkinson, A Rugetski, C Baxter, KS Boersma, CC Carey, PB McIntyre, JW Moore, WH Nowlin, and CC Vaughn. 2012. Organized oral session: Impacts of species addition and species loss on ecosystem function in freshwater systems. Bulletin of the Ecological Society of America. 93:402-408. Jones, TA, MM Chumchal, RW Drenner, GN Timmins§, and WH Nowlin. 2012. Bottom-up nutrient and top-down fish impacts on contaminant flux from aquatic ecosystems. Environmental Toxicology and Chemistry. 32:1-7. Brown, DR, B DeVolld, WH Nowlin, and M Forstner. 2012. Ants in Houston toad habitat: annual activity and responses to canopy cover and fire. Journal of Fish and Wildlife Management. 3:870-876. Scott, SE, CL Pray†, WH Nowlin, and Y Zhang. 2012. Effects of native and introduced consumers on stream ecosystem function. Aquatic Sciences. 74:793-808. Weston Hugh Nowlin – Curriculum Vitae 2 Henderson, BL, MM Chumchal, RW Drenner, PH Diaz†, and WH Nowlin. 2012. Mercury contamination of macroinvertebrates from grassland ponds with and without fish. Environmental Toxicology and Chemistry. 31:870-876. Becker, JC*, AW Groeger, WH Nowlin, MM Chumchal, and D Hahn. 2011. Spatial variability in the speciation and bioaccumulation of mercury in an arid subtropical reservoir ecosystem. Environmental Toxicology and Chemistry. 30:2300-2311. Pound, KL, TH Bonner, WH Nowlin, and DG Huffman. 2011. Trophic ecology of a nonnative population of suckermouth catfishes (Hypostomus) in a central Texas spring-fed stream. Environmental Biology of Fishes. 90:277-285. Davies, JM, WH Nowlin, B Matthews, and A Mazumder. 2010. Temporal discontinuity of nutrient limitation in plankton communities. Aquatic Sciences. 72:393-402. Smith, A†, A Abuzeineh*, MM Chumchal, TH Bonner, and WH Nowlin. 2010. Mercury contamination of the fish community of a semi-arid and arid river system: Spatial variation and the influence of environmental gradients. Environmental Toxicology and Chemistry. 29:1762-1772. Caston CB†, WH Nowlin, A Gaulke, and MJ Vanni. 2009. The relative importance of heterotrophic bacteria to pelagic ecosystem dynamics varies with reservoir trophic state. 2009. Limnology and Oceanography 54:2143-2156. Pray, CL*, WH Nowlin, and MJ Vanni. 2009. Deposition and decomposition of periodical cicadas (Homoptera: Cicadidae) in woodland aquatic systems. Journal of the North American Benthological Society 28:181-195. Nowlin, WH, MJ Vanni and LH Yang. 2008. Comparing resource pulses in aquatic and terrestrial systems. Ecology 89:647-659. Nowlin, WH, MJ González, MHH Stevens, MJ Vanni, MW Fields and JJ Valente. 2007. Allochthonous subsidy of periodical cicadas affects dynamics and stability of pond communities. Ecology 88:2174-2186. Nowlin, WH, J-M Davies and A Mazumder. 2007. Planktonic phosphorus pool sizes and cycling efficiency in coastal and interior British Columbia lakes. Freshwater Biology 52: 860-877. Nowlin, WH, RW Drenner, KR Guckenberger, M Lauden, T Alonso, JE Fennell and JL Smith. 2006. Gape limitation, prey size refuges and the top-down impacts of piscivorous largemouth bass in shallow pond ecosystems. Hydrobiologia 563: 357-369. Chumchal, M, WH Nowlin, RW Drenner and S Mann. 2005. Biomassdependent effects of common carp on water quality in shallow ponds. Hydrobiologia 545: 271-277. Weston Hugh Nowlin – Curriculum Vitae 3 Nowlin, WH, JL Evarts and MJ Vanni. 2005. Release rates and potential fates of nitrogen and phosphorus from sediments in a eutrophic reservoir. Freshwater Biology 50: 301-322. Davies, J-M, WH Nowlin and A Mazumder. 2004. Temporal changes in nitrogen and phosphorus co-deficiency in plankton in lakes of coastal and interior British Columbia. Canadian Journal of Fisheries and Aquatic Sciences 61: 1538-1551. Nowlin, WH, J-M Davies, RN Nordin and A Mazumder. 2004. Effects of water level fluctuation and short-term climate variation on thermal and stratification regimes of a British Columbia reservoir and lake. Lake and Reservoir Management 20: 91-109. Davies, J-M, WH Nowlin and A Mazumder. 2004. Variation in temporal [14C] plankton photosynthesis among warm monomictic lakes of coastal British Columbia. Journal of Plankton Research 26: 763-778. Nowlin, WH, and RW Drenner. 2000. Context-dependent effects of bluegill on experimental mesocosm communities. Oecologia 122: 421-426. Technical Publications Spafard, MA, WH Nowlin, J-M Davies and A Mazumder. 2002. A morphometric atlas of selected lakes in southern British Columbia: Vancouver Island, Saltspring Island and the Kooteney region. National Sciences and Engineering Research Council (NSERC), Industrial Research Chair Program, Environmental Management of Drinking Water, University of Victoria. Pending External Funding Support (amounts in US$D) Lakes as sentinels of climate change. National Science Foundation – Macrosystem Biology. $6,496,792 Awarded External Funding Support (amounts in US$D) Plastron Efficiency of the Comal Springs Riffle Beetle. WH Nowlin, B Schwartz, T Hardy, and R Gibson. Edwards Aquifer Authority – Habitat Conservation Plan. Received May 2014. $48,000 Upper San Marcos River Watershed Protection Plan. WH Nowlin and B Schwartz. Texas Commission on Environmental Quality/United States Environmental Protection Agency 319(h) Grant. Received January 2013. $30,000 Establishment of the Upper San Marcos River Observing System. B Schwartz (PI), WH Nowlin (PI), TH Bonner (Co-PI), T Hardy (Co-PI), and A Sansom (CoPI). Exceptional Line Item in the State of Texas Budget (2009 – 2011). Received February 2010. $1,200,000 Weston Hugh Nowlin – Curriculum Vitae 4 Ecological Values and Ecosystem Services of Watersheds in the Lower Rio Grande River. TH Bonner (PI), WH Nowlin (Co-PI), and T Hardy (Co-PI). United States Department of Agriculture (Sustainable Agricultural Water Conservation in the Rio Grande Project). Received July 2009. $85,000 Effects of Invasive Species on the Biogeochemistry of Hyporheic Water from Sandbars: A Comparison Restored and Unrestored Areas in Big Bend National Park. WH Nowlin (PI), K Urbanczyk (Co-PI), and J Bennett (Co-PI). United States Department of Agriculture (Sustainable Agricultural Water Conservation in the Rio Grande Project). Received July 2009. $37,843 Spring Lake Nutrient Sources Identification and Nutrient Management Plan. WH Nowlin (PI), B Schwartz (Co-PI), and A Sansom (Co-PI). Texas Commission on Environmental Quality/United States Environmental Protection Agency 319(h) Grant. Received March 2009. $293,689 Project Flowing Waters. J Westerlund (PI), T. Bonner (Co-PI), WH Nowlin (CoPI), R. Earl (Co-PI), and P. Guettner (Co-PI). National Science Foundation: Graduate Teaching Fellows in K-12 Education (GK-12). Received January 2008. $2,320,660 Building adaptive capacity and the ecological values of watersheds of the Lower Brazos River Basin – TH Bonner (PI), WH Nowlin (Co-PI), Y Zhang (Co-PI) and V Lopes (Co-PI) in cooperation with The Nature Conservancy of Texas. The Houston Foundation. Received January 2008. $600,000 Bigclaw River Shrimp in San Marcos River: Invasive Species’ Impact. Y Zhang (PI) and WH Nowlin (Co-PI). Texas Parks and Wildlife State Wildlife Grant Program. Received October 2007. $68, 640 Trophic ecology and habitat associations of the threatened San Marcos salamander (Eurycea nana) – WH Nowlin (PI), JN Fries (Co-PI) and ML Alexander (Co-PI). United States Geological Survey. Received May 2007. $25,844 Sources and pathways of energy and nutrients supporting the Rio Grande biotic community - WH Nowlin (PI) and TH Bonner (Co-PI). United States Department of Agriculture (Sustainable Agricultural Water Conservation in the Rio Grande Project). Received March 2006. $90,404 Reproductive Ecology and Culture Techniques of the Devil’s River Minnow WH Nowlin (Single PI). United States Fish and Wildlife Service Cooperative Agreement. Received June 2006. $8,000 Invasion of Suckermouth Catfish in the San Marcos River Ecosystem – WH Nowlin (PI) and TH Bonner (Co-PI). United States Fish and Wildlife Service, Collaborative Conservation Project Award Initiative (CCPAI). Received March 2006. $5,000 Weston Hugh Nowlin – Curriculum Vitae 5 Awarded Internal Funding Support Transfer of Mercury and Methylmercury Across Ecosystem Boundaries in Arid River Landscapes - WH Nowlin (Single PI). Texas State University Research Enhancement Program. Received December 2007. $7,984 Ecosystem engineering and nutrient cycling effects of native and exotic species in the San Marcos River ecosystem - Y Zhang (PI) and WH Nowlin (Co-PI). Texas State University Research Enhancement Program Grant. Received December 2006. $16,000 The Relative Importance of Bacteria and Algae in Reservoir Carbon Cycling – WH Nowlin (Single PI). Texas State University Research Enhancement Program Grant. Received December 2005. $6, 940 Presentations at Professional Meetings *, †, and ‡ denote PhD, Masters, and undergraduate students in my lab, respectively Becker, JC*, WH Nowlin, and D Hahn. 2013. Biogeography and ecosystem function of major aquatic bacterial groups in a Gulf Slope (TX) river system. Texas Academy of Science. Kerrville Texas. Swink, AP†, WH Nowlin, and BF Schwartz. 2013. Storm mediated changes in nitrogen and phosphorus uptake length in a spring fed river. Texas Academy of Science. Kerrville Texas. Swink, AP†, WH Nowlin, and BF Schwartz. 2013. Storm-mediated changes in phosphorus uptake in a spring fed river. Annual Great Plains Limnology Conference. Fayetteville, Arkansas. Becker, JC*, WH Nowlin, and D Hahn. 2012. Spatial patterns in microbial diversity in a large river system. Annual Great Plains Limnology Conference. Fayetteville, Arkansas. Rodibaugh, KJ†, WH Nowlin and JC Becker*. 2012. Bacterially Mediated Carbon and Nutrient Dynamics in a Highly Impacted River System. Annual Great Plains Limnology Conference. Fayetteville, Arkansas. Nowlin, WH, C LeBeouf†, S Scott, CL Pray†, and Y Zhang. 2012. Invasion potential and ecosystem-level impacts of non-native fishes in a riverine system. Ecological Society of America. Portland, Oregon. (Invited talk part of a Special Session). Abuzeineh, AA*, JR Troy, MM Chumchal, MC Green, and WH Nowlin. 2012. Transfer of mercury across ecosystem boundaries in arid streams. Ecological Society of America. Portland, Oregon. Nowlin, WH, CL Pray†, SE Scott, CM LeBoeuf†, KL Pound and Y Zhang. 2012. Ecosystem-level consequences of the addition of invasive suckermouth catfish (Loricariidae) to riverine ecosystems. Society for Freshwater Science. Louisville, Kentucky. (Invited talk part of a Special Session) Weston Hugh Nowlin – Curriculum Vitae 6 Becker, JC*, WH Nowlin BJ Labay, and KJ Rodibaugh†. 2012. Influence of land-use and physiography on nutrient concentration in a large river system. Society for Freshwater Science. Louisville, Kentucky. Chumchal, MM, TA Jones, RW Drenner, WH Nowlin, and GN Timmins§. 2012. Bottom-up nutrient and top-down fish impacts on contaminant flux from aquatic ecosystems. Society for Freshwater Science. Louisville, Kentucky. Abuzeineh, AA*, JR Troy, MM Chumchal, MC Green, and WH Nowlin. 2011. Transfer of mercury across ecosystem boundaries in arid streams. Society of Environmental Toxicology and Chemistry. Boston, Massachusetts. Becker, J.C. *, W.H. Nowlin, K.J. Rodibaugh†, and B.J. Labay. 2011. Influence of land use at multiple spatial scales on nutrients and ecosystem function in a large river system. 4th Oklahoma-Texas Aquatic Research Group and Great Plains Limnological Conference. University of Oklahoma Biological Station, Lake Texoma, Oklahoma. Rodibaugh, K.J. †, W.H. Nowlin and J.C. Becker*. 2011. Bacterially mediated carbon and nutrient dynamics in a highly impacted river system. 4th OklahomaTexas Aquatic Research Group and Great Plains Limnological Conference. University of Oklahoma Biological Station, Lake Texoma, Oklahoma. McMillan, S., TH Bonner, WH Nowlin, and JN Fries. 2011. Reproductive ecology of two sympatric cyprinids in the Rio Grande basin, Texas. American Fisheries Society. Seattle, Washington. Hardy, T, W. Nowlin, B. Schwartz, and T. Bonner. 2011. San Marcos River Observing System. American Fisheries Society. Seattle, Washington. Hardy, T.B., W.H. Nowlin, B. Schwartz, and T. Bonner. 2011. San Marcos Observing System: Integrating Physical, Chemical, and Biological Data at High Resolution Spatial and Temporal Scales. American Fisheries Society. Seattle, Washington. Abuzeineh, A.A. *, W. H. Nowlin, A. Smith†, T. C. Heard, and T. H. Bonner. 2011. Organic matter sources supporting communities of an arid and semi-arid riverine system: The lower Rio Grande drainage. Ecological Society of America, Austin, Texas. Henderson, B.L., M.M. Chumchal, R.W. Drenner, P. Diaz†, Y. Deng, and W.H. Nowlin. 2011. Mercury contamination of macroinvertebrates from ponds with and without fish. Ecological Society of America, Austin, Texas. Henderson, B.L., M.M. Chumchal, R.W. Drenner, P. Diaz†, Y. Deng, and W.H. Nowlin. 2011. Effects of fish on mercury contamination of macroinvertebrate communities of grassland ponds. Joint Meeting of the Oklahoma-Texas Aquatics Research Group and the Great Plain’s Limnology Society, University of Oklahoma Biological Station, Oklahoma. Weston Hugh Nowlin – Curriculum Vitae 7 Henderson, B.L., M.M. Chumchal, R.W. Drenner, P. Diaz†, Y. Deng, and W.H. Nowlin. 2011. Mercury contamination of macroinvertebrates from ponds with and without fish at the LBJ National Grassland, Texas. 10th International Conference on Mercury as a Global Pollutant, Halifax, Nova Scotia, Canada. Henderson, B.L., M.M. Chumchal, R.W. Drenner, P. Diaz†, Y. Deng, and W.H. Nowlin. 2011. Mercury contamination of macroinvertebrates from ponds with and without fish at the LBJ National Grassland, Texas. South Central Regional Chapter of the Society of Environmental Toxicology and Chemistry, Denton, Texas. Park, A. J., M.R. Sawey, M.M. Chumchal, R.W. Drenner, Y. Deng, and W.H. Nowlin. 2011. Biomagnification of mercury within the macroinvertebrate community of Caddo Lake. South Central Regional Chapter of the Society of Environmental Toxicology and Chemistry, Denton, Texas. Becker, J.C. *, W.H. Nowlin, K.J. Rodibaugh†, and B.J. Labay. 2011. Influence of land use at multiple spatial scales on nutrients and ecosystem function in a large river system. Ecological Society of America, Austin, TX. Rodibaugh, K.J. †, W.H. Nowlin and J.C. Becker*. 2011. Bacterially Mediated Carbon and Nutrient Dynamics in a Highly Impacted River System. Ecological Society of America, Austin, TX. Abuzeineh, A.A. *, W. H. Nowlin, A. Smith†, T. C. Heard, and T. H. Bonner. 2011. Organic matter sources supporting communities of an arid and semi-arid riverine system: The lower Rio Grande drainage. Ecological Society of America, Austin, Texas. Becker, J.C. *, W.H. Nowlin, K.J. Rodibaugh†, and B.J. Labay. 2011. Influence of land use at multiple spatial scales on nutrients and ecosystem function in a large river system. ASLO, San Juan, Puerto Rico. Rodibaugh, K.J. †, W.H. Nowlin and J.C. Becker*. 2011. Bacterially Mediated Carbon and Nutrient Dynamics in a Highly Impacted River System. ASLO Aquatic Science, San Juan, Puerto Rico. Bonner, T. H., J. F. Westerlund, W. H. Nowlin, and R. A. Earl. 2010. Graduate training through Project Flowing Waters at Texas State University: quantifying benefits to participating graduate students. NSF GK-12 Western Region Annual Meeting. Denver, Colorado. Nowlin, WH, J Westerlund, TH Bonner, and R Earl. 2010. Graduate training through Project Flowing Waters: Challenges at Texas State University. Ecological Society of America, Pittsburgh, Pennsylvania. Lash, FP, Y Zhang, WH Nowlin, and TH Bonner. 2010. Impacts of land use in the lower Brazos River on benthic macroinvertebrate diversity. Joint ASLO (Advancing the Sciences of Limnology and Oceanography) and North American Benthological Society (NABS) Annual Meeting, Santa Fe, New Mexico. Weston Hugh Nowlin – Curriculum Vitae 8 Nowlin, WH, CL Pray†, SE Scott, CM LeBoeuf†, KL Cohen, and Y Zhang. 2010. Ecosystem-level impacts of invasive suckermouth catfish (Loricariidae) on a spring-influenced river. Joint ASLO (Advancing the Sciences of Limnology and Oceanography) and North American Benthological Society (NABS) Annual Meeting, Santa Fe, New Mexico. Henderson, BL, MM Chumchal, RW Drenner, PH Diaz†, Y Deng, and WH Nowlin. 2010. Mercury contamination of macroinvertebrates from ponds with and without fish at the LBJ National Grassland, Texas USA. Joint ASLO (Association for the Sciences of Limnology and Oceanography) and North American Benthological Society (NABS) Annual Meeting, Santa Fe, New Mexico. Becker, JC*, WH Nowlin, KJ Rodibaugh†, and BJ Labay. 2010. Spatial patterns of nutrient concentration and ecosystem function in a western Gulf slope river system. Joint ASLO (Association for the Sciences of Limnology and Oceanography) and North American Benthological Society (NABS) Annual Meeting, Santa Fe, New Mexico. LeBoeuf, CM†, Y Zhang, and WH Nowlin. 2010. The effects of invasive species and eutrophication on riverine nutrient dynamics. Southwestern Association of Naturalists (SWAN) Annual Meeting, Junction, Texas. Park, A, M Sawey, MM Chumchal, RW Drenner, Y Deng, J Wadlington, and WH Nowlin. 2010. Spatial variation of Hg, 15N, and 13C in sediment and macroinvertebrates in Caddo Lake. Texas Academy of Science Annual Meeting, Stephenville, Texas. BL Henderson, MM Chumchal, RW Drenner, PH Diaz†, Y Deng, and WH Nowlin. 2010. Mercury concentration in macroinvertebrates from grassland ponds with and without fish communities. Texas Academy of Science Annual Meeting, Stephenville, Texas. WH Nowlin, CL Pray†, SE Scott, CM LeBoeuf†, KL Cohen, TH Bonner, and Y Zhang. 2010. Ecosystem-level impacts of invasive suckermouth catfish (Loricariidae) on the San Marcos River, Texas. Texas Chapter of the American Fisheries Society, Athens, Texas. Smith, A†, WH Nowlin, M. Chumchal, and A Abuzeineh*. 2010. Mercury contamination of the fish community of a semi-arid and arid river system: spatial variation and the influence of environmental gradients. Texas Chapter of the American Fisheries Society, Athens, Texas. JC Becker*, AW Groeger, MM Chumchal, WH Nowlin, and D Hahn. 2010. Spatial variability in the distribution and bioaccumulation of mercury in a subtropical reservoir system. Texas Chapter of the American Fisheries Society, Athens, Texas. Becker, JC*, AW Groeger, MM Chumchal, and WH Nowlin. 2009. Distribution and bioaccumulation of mercury in a subtropical reservoir system. Society for Environmental Toxicology and Chemistry (SETAC) – North America, 29th Annual Meeting, New Orleans, Louisiana. Weston Hugh Nowlin – Curriculum Vitae 9 Smith, A†, WH Nowlin, MM Chumchal, and A Abuzeineh*. 2009. Mercury contamination of the Rio Grande fish community: influence of spatial variation and food web structure. South Central Chapter of the Society of Environmental Toxicology and Chemistry (SETAC Annual Meeting), San Marcos, Texas. Nowlin, WH, CB Caston†, A Gaulke, and MJ Vanni. 2009. Stoichiometry and limitation of bacteria and phytoplankton along a productivity gradient. ASLO (Advancing the Sciences of Limnology and Oceanography), Nice, France. Westerlund, J.F., TH Bonner, WH Nowlin, and RA Earl. 2008. NSF GK-12: Project Flowing Waters. North Central Association for Science Teacher Education, (NC-ASTE), Winona, Minnesota. Smith, A†, WH Nowlin, MM Chumchal, and A Abuzeineh*. 2008. Mercury contamination of the Rio Grande fish community: influence of spatial variation and food web structure. Society for Environmental Toxicology and Chemistry (SETAC) – North America, 29th Annual Meeting, Tampa, Florida. Smith, A†, WH Nowlin, MM Chumchal, and A Abuzeineh*. 2008. Mercury contamination of the Rio Grande fish community: influence of spatial variation and food web structure. Oklahoma-Texas Aquatic Research Group/Great Plains Limnology Conference, Kingston, Oklahoma. Pray, CL†, SE Scott, WH Nowlin, and Y Zhang. 2008. Ecosystem engineering effects of native and non-native consumers in a river ecosystem. Oklahoma-Texas Aquatic Research Group/Great Plains Limnology Conference, Kingston, Oklahoma. Diaz, PH* and WH Nowlin. 2008. Influence of productivity, size, and hydroperiod on aquatic macroinvertebrates in isolated wetlands. Oklahoma-Texas Aquatic Research Group/Great Plains Limnology Conference, Kingston, Oklahoma. Caston, CB†, WH Nowlin, A Gaulke, and MJ Vanni. 2008. Nutrient cycling in pelagic ecosystems: The relative importance of heterotrophic bacteria and phytoplankton changes along a trophic gradient. Oklahoma-Texas Aquatic Research Group/Great Plains Limnology Conference, Kingston, Oklahoma. Becker, JC†, AW Groeger, MM Chumchal, and WH Nowlin. 2008. Distribution and bioaccumulation of mercury in a subtropical reservoir system. OklahomaTexas Aquatic Research Group/Great Plains Limnology Conference, Kingston, Oklahoma. Nowlin, WH, CB Caston†, A Gaulke, and MJ Vanni. 2008. Stoichiometry and nutrient limitation of bacterio- and phytoplankton across a reservoir productivity gradient: implications for competition intensity. Oklahoma-Texas Aquatic Research Group/Great Plains Limnology Conference, Kingston, Oklahoma. Smith, A†, WH Nowlin, MM Chumchal, and A Abuzeineh*. 2008. Mercury contamination of the Rio Grande fish community: influence of spatial variation Weston Hugh Nowlin – Curriculum Vitae 10 and food web structure. South Central Chapter of the Society of Environmental Toxicology and Chemistry (SC-SETAC) Annual Meeting, Clear Lake, Texas. Becker, JC†, AW Groeger, MM Chumchal, and WH Nowlin. 2008. Distribution and bioaccumulation of mercury in a subtropical reservoir system. South Central Chapter of the Society of Environmental Toxicology and Chemistry (SC-SETAC) Annual Meeting, Clear Lake, Texas. Caston, CB† and WH Nowlin. 2007. Does the relative importance of bacteria in reservoir nutrient cycling vary with productivity? Texas River and Reservoir Management Society (TRRMS) and South Central Society of Environmental Toxicology and Chemistry (SC-SETAC), Nacogdoches, Texas. Cohen, KL, TH Bonner, and WH Nowlin. 2007. Food habits of the nonindigenous suckermouth catfish in the San Marcos River, Texas: A concern for spring endemics? American Fisheries Society, San Francisco California. Cohen, KL, TH Bonner, and WH Nowlin. 2007. Food habits of the nonindigenous suckermouth catfish in the San Marcos River, Texas: a concern for spring endemics? Texas Chapter of the American Fisheries Society, Lake Jackson, Texas. Nowlin, WH, A Gaulke, and MJ Vanni. 2007. The relative importance of bacteria and algae in pelagic nutrient cycling of reservoir ecosystems. ASLO (Association for the Sciences of Limnology and Oceanography), Santa Fe, New Mexico. Davies, JM, WH Nowlin, B Matthews and A Mazumder. 2006. A temporal discontinuity of nutrient deficiency and limitation, the role of deficiency in cyanobacterial bloom formation. ASLO (Association for the Sciences of Limnology and Oceanography), Victoria, British Columbia. Nowlin, WH, MHH Stevens, M.J. Vanni, M.J. Gonzalez, and M.W. Fields. 2005. Effects of a pulsed allochthonous subsidy from a 17-year periodical cicada emergence on woodland pond communities. Oklahoma – Texas Aquatic Research Group and Texas River and Reservoir Management Society Meeting, Oklahoma Biological Station, Kingston, Oklahoma Valente, JJ, MJ Gonzalez, WH Nowlin, MJ Vanni, MW Fields, and MHH Stevens. 2005. Effects of the 17-year periodical cicada emergence on zooplankton communities in woodland ponds. Ecological Society of America, Montréal, Quebec Pauly, L, MW Fields, C Koenig, C. Hwang and WH Nowlin. 2005. Allochthonous input effects on the bacterial community of an aquatic mesocosm. Ecological Society of America, Montréal, Quebec. Nowlin, WH, MHH Stevens, M.J. Vanni, M.J. Gonzalez, J.J. Valente and M.W. Fields. 2005. Effects of a pulsed allochthonous subsidy from a 17-year periodical cicada emergence on woodland pond communities. Ecological Society of America, 2005, Montréal, Quebec. Symposium: Resource pulses in space and Weston Hugh Nowlin – Curriculum Vitae 11 time: Linking species, communities, and ecosystems, Ecological Society of America, Montréal, Quebec Pray, CL, WH Nowlin, MJ Vanni, MHH Stevens, MJ González, MW Fields. 2005. Deposition and decomposition of litter associated with the emergence of 17-year periodical cicadas in woodland ponds and streams. Ecological Society of America, Montréal, Quebec Nowlin, WH, J-M Davies and A Mazumder. 2004. The relative importance of “new” and “recycled” phosphorus sources in lakes of varying trophy in British Columbia, Canada. Ecological Society of America, Portland, Oregon. Mazumder, A, R Nordin, B Matthews, J-M Davies, WH Nowlin, PC Furey, C Meays and K Broersma. 2004. Integrated water and watershed management for sustainable clean and healthy water. The International Congress of Limnology (SIL), Helsinki, Finland. Nowlin, WH, J-M Davies and A Mazumder. 2002. Effects of seasonal drawdown on a drinking water reservoir: response of pelagic phosphorus pathways. American Society of Limnology and Oceanography, Victoria, British Columbia, Canada. Mazumder, A, Y Liang, J-M Davies, WH Nowlin, P Furey and B Matthews. 2002. Loss of ecosystem and watershed quality: a serious threat to sustainable clean and healthy drinking water. American Society of Limnology and Oceanography, Victoria, British Columbia, Canada. Davies, J-M, WH Nowlin and A Mazumder. 2002. Phytoplankton nutrient status: implications of plankton size. American Society of Limnology and Oceanography, Victoria, British Columbia, Canada. Nowlin, WH, J-M Davies and A Mazumder. 2002. Effects of seasonal drawdown on a drinking water reservoir: responses of pelagic phosphorus pathways. Society of Canadian Limnologists, Vancouver, British Columbia, Canada. Nowlin, WH, RW Drenner, M Chumchal, and S Mann. 2000. Biomassdependent effects of common carp on water quality: Implications for biomanipulation of Texas reservoirs. American Society of Limnology and Oceanography, Copenhagen, Denmark. Basu, BK, Y Liang, P Caron, J-M Davies, WH Nowlin and A Mauzumder. 2000. Environmental management of drinking water: An ecosystem and watershed approach. American Society of Limnology and Oceanography, Copenhagen, Denmark. Drenner, RW, JL Smith, S Mann, M Chumchal, JM Fennell, WH Nowlin and CE Edwards. 2000. Estimating the densities of common carp and their biomassdependent effects on ponds. Texas Chapter of the American Fisheries Society, Bossier City, Louisiana, USA. Weston Hugh Nowlin – Curriculum Vitae 12 Mazumder, A, BK Basu, Y Liang, P Caron, J-M Davies and WH Nowlin. 2000. The environmental management of drinking water: an overview of the NSERCIRC program at the University of Victoria. Society of Canadian Limnologists, Fredericton, New Brunswick, Canada. Drenner, RW, WH Nowlin, KR Guckenberger, M Lauden, JE Fennell and JL Smith. 1999. Effects of piscivorous largemouth bass on water quality. Texas River and Reservoir Management Society, College Station, Texas, USA. Nowlin, WH and RW Drenner. 1998. Effects of bluegill on experimental mesocosm communities in the presence and absence of a fish assemblage. Texas River and Reservoir Management Society/North Texas Limnology Group, Denton, Texas, USA. Nowlin, WH and RW Drenner. 1998. Effects of bluegill on experimental mesocosm communities in the presence and absence of a fish assemblage. American Society of Limnology and Oceanography/Ecological Society of America, St. Louis, Missouri, USA. Conference Proceedings Mock, L. BF Schwartz, WH Nowlin, BT Hutchins, T Hardy, TH Bonner. 2011. The role of metrology in monitoring and preserving the water quality in the Edwards Aquifer in Central Texas. Proceedings of the 2011 National Conference of Standards Laboratories International (NCSLI) Symposium. August 21-25, 2011, National Harbor, MD. Invited Seminars University of Southern Mississippi, Department of Biological Science – November 2012. Creatures great and small: The role of organisms in riverine nutrient cycling. University of North Texas, Department of Biological Science – January 2012. Creatures great and small: The role of organisms in ecosystem nutrient dynamics. The University of Texas – Austin, School of Biological Sciences, Section of Integrative Biology – April 2010. Creatures great and small: The importance of organisms in ecosystem nutrient dynamics. Austin College, Department of Biology, Sherman, Texas – October 2006. Picoplankton, piscivores and periodical cicadas: research in aquatic ecology. Texas State University, Aquatic Biology Society, San Marcos, Texas – October 2005. Effects of a pulsed allochthonous subsidy from a 17-year periodical cicadas emergence on woodland pond communities. Miami University, Department of Zoology, Oxford, Ohio – Miami Department of Zoology REU program speaker, July 2005. The effects of 17-year periodical cicadas on woodland aquatic ecosystems: An overview of summer research in the Miami ECO-REU program. Weston Hugh Nowlin – Curriculum Vitae 13 Bowling Green State University, Department of Biological Sciences, Bowling Green, Ohio – January 2005. The relative importance of “new” and “recycled” phosphorus sources in lakes of varying trophy in British Columbia, Canada. Weston Hugh Nowlin – Curriculum Vitae 14 Students Directed and Advised Graduate Student Theses and Dissertations Directed A Everett. In progress. TBD. Texas State University, MS. LA Loney. In progress. Nutrient recycling and stoichiometry of stygobiont invertebrates in a karst aquifer system. Texas State University, MS. AP Swink. In progress. Phosphorus uptake in karstic spring-fed rivers of central Texas. Texas State University, MS. K Hoesel. In progress. Effects of invasive floating vegetation on the nutrient dynamics of a spring-influenced ecosystem. Texas State University, MS. JC Becker. Graduated August 2013. Biogeochemistry and microbial diversity of a Gulf-slope drainage. Texas State University. Ph.D. KJ Rodibaugh. Graduated December 2012 Landscape-level patterns in ecosystem metabolism of an arid and semi-arid river system. Texas State University, MS. AA Abuzeineh. Graduated December 2012. Cross system transport of organic matter and contaminants in semi-arid and arid lotic ecosystems. Texas State University. Ph.D. CM LeBoeuf. Graduated May 2010. The effects of invasive species and eutrophication on the nutrient dynamics of a spring-fed river ecosystem. Texas State University. M.S. PH Diaz. Graduated May 2010. Trophic ecology and habitat use of the San Marcos Salamander. Texas State University. MS. C L Pray. Graduated December 2009. Ecosystem impacts of the exotic armored catfish in the San Marcos River. Texas State University. MS. A Smith. Graduated May 2009. Spatial distribution and factors affecting Hg levels in food webs of the Rio Grande and Pecos River. Texas State University. MS. CB Caston. Graduated May 2008. The relative importance of bacteria and algae in reservoir carbon cycling. Texas State University. M.S. Undergraduate Student Projects Directed PH Diaz. 2006. The influence of productivity, size, and hydroperiod on species richness in geographically isolated wetlands. Texas State University. Recipient of Francis Rose Undergraduate Research Award 2006 and winner of Best Undergraduate Speaker at the Texas State University Department of Biology Student Research Colloquium. Weston Hugh Nowlin – Curriculum Vitae 15 CL Pray. 2004. Deposition and decomposition of periodical cicada detritus in woodland aquatic ecosystems: potential impacts on nutrient cycling. Miami University, Ohio. Service as a Member of Dissertation and Thesis Committees Stinson, C - MS, Aquatic Resources, Department of Biology, Texas State University. In progress. Hutchins, B - PhD, Aquatic Resources, Department of Biology, Texas State University. In progress. Whitney, S - PhD, Aquatic Resources, Department of Biology, Texas State University. In progress. Tobin, B - PhD, Aquatic Resources, Department of Biology, Texas State University. Completed 2013 Brown, D – PhD, Aquatic Resources, Department of Biology, Texas State University. Completed 2013 Cocke, W – MS, Biology, Department of Biology, Texas Christian University. Completed 2012. Gerard, B – MS, Aquatic Resources, Department of Biology, Texas State University. Completed 2012. Bean, P – PhD, Aquatic Resources, Department of Biology, Texas State University. Completed 2012. Lash, F – MS, Aquatic Resources, Department of Biology, Texas State University. Completed 2011 Shattuck, Z. – MS, Aquatic Resources, Department of Biology, Texas State University, Competed 2010 Scott, SE - MS – Aquatic Resources, Department of Biology, Texas State University, Completed 2009 Folb, C – MS, Aquatic Resources, Department of Biology, Texas State University, Completed 2009 Becker, JC – MS, Aquatic Resources, Department of Biology, Texas State University, Completed 2008 Meyer, J – MS, Aquatic Resources, Department of Biology, Texas State University, Completed 2008 Heard, T - MS, Aquatic Resources, Department of Biology, Texas State University, Completed 2008 Cohen, K - MS, Aquatic Resources, Department of Biology, Texas State University, Completed 2008 Weston Hugh Nowlin – Curriculum Vitae 16 Marfurt, R - MS, Aquatic Resources, Department of Biology, Texas State University, Completed 2007 Runyan, D - MS, Aquatic Resources, Department of Biology, Texas State University, Completed 2007 Honors and Awards Texas State University Presidential Award for Scholarly/Creative Activities – Assistant Professor. 2008. Recognized as top researcher among all Assistant Professors from across all colleges/schools at the University. Graduate Student Tuition Fellowship – University of Victoria, Department of Graduate Studies. Received award every semester from September 1999 September 2003 Student Travel Award – American Society of Limnology and Oceanography (ASLO) Conference 2000 in Copenhagen, Denmark Outstanding Graduate Student of 1998 – Texas Christian University, Department of Biology Adkins Research Fellowship to Graduate Students – Texas Christian University, May - September 1997 Professional Service and Activities Departmental and University Service Director, Aquatic Resources MS Program (May 2012 – Present) Chair, Stream Ecologist Faculty Search Committee, Department of Biology, Texas State University (September 2013 – Present) Chair, Ten-Year Strategic Hiring Plan Committee, Department of Biology, Texas State University (September 2013 – Present) Currently serve on the Awards Day Committee (Chair), Budget Committee, College Recruiting Committee, Tenure Tracking Committee, and the Spring Lake Environmental Committee Professional Memberships ASLO (Advancing the Science of Limnology and Oceanography), ESA (Ecological Society of America), Society for Environmental Toxicology and Chemistry (SETAC), NALMS (North American Lake Management Society), SCL (Society of Canadian Limnologists) Leadership in Professional Societies President - South Central Chapter of the Society for Environmental Toxicology and Chemistry, 2012-2013 Vice President, South Central Chapter of the Society for Environmental Toxicology and Chemistry, 2010-2011 Weston Hugh Nowlin – Curriculum Vitae 17 Board Member, South Central Chapter of the Society for Environmental Toxicology and Chemistry, 2009 – Current Served as Manuscript Reviewer Hydrobiologia, Ecology Letters, Oikos, Environmental Toxicology and Chemistry, Hydrobiologia, Oecologia, Lakes and Reservoirs: Research and Management, Aquatic Conservation: Marine and Freshwater Ecosystems, Limnology and Oceanography, Freshwater Biology, Southwestern Naturalist, American Midland Naturalist, Transactions of the American Fisheries Society, Journal of Applied Biology, Water Resources and Management, Journal of the North American Benthological Society, Lake and Reservoir Management Served as Research Grant Proposal Reviewer Israel Science Foundation (ISF), National Science Foundation (NSF) Membership on Committees in Professional Organizations American Society of Limnology and Oceanography Education Sub-committee of the Education and Human Resources Committee (January 2003 – January 2005) – Program designed to advance education of the scientific fields of limnology and oceanography across a broad range of students from kindergarten to graduate students. Committee activities include creation of web-based courses, education outreach programs, creation of teaching tools and a web-based image library. Organization of Professional Meetings Oklahoma – Texas Aquatic Research Group (OTARG)/Great Plains Limnology Conference (GPLC), October 2011. Organization and hosting of tri-annual meeting at The University of Oklahoma Biological Station. Society for Environmental Chemistry and Toxicology, May 2009, Organization and hosting of the annual meeting of the South Central Chapter of the Society for Environmental Chemistry and Toxicology at Texas State University. Joint Ecological Society of America (ESA) and International Congress of Ecology (INTECOL), Symposium Co-Organizer, 2005 Annual Meeting in Montreal, Quebec, Canada – Resource pulses in space and time: Linking species, communities and ecosystems. American Society of Limnology and Oceanography, Summer 2002 meeting in Victoria, BC, Canada – Organize and coordinate activities for the ASLO Minorities in the Aquatic Sciences (MAS) Program. Community Service Technical Science Advisor, Upper San Marcos Coordinating Group (stakeholder group formed as part of the Watershed Protection Plan for the Upper San Marcos). I additionally attended the City of San Marcos Mater Planning Meetings as the group representative. Weston Hugh Nowlin – Curriculum Vitae 18 Research Experience Miami University Postdoctoral Research Scholar – Miami University January 2004 Effects of a pulsed nutrient subsidy into small woodland streams and semi-permanent ponds associated with an emergence of periodical 17-year cicadas. Funded through a NSF-SGER (Small Grant for Exploratory Research) grant and the NSF REU (Research Experience for Undergraduates) program at Miami University. Graduate Research Assistant – University of Victoria September 1999 - December 2003 Research focusing on planktonic phosphorus cycling in lakes of varying productivity. Part of a large NSERC Research Chair program on the Environmental Management of Drinking Water. Graduate Research Assistant – Texas Christian University May - September 1998 Biomass dependent effects of common carp (Cyprinus carpio) on water quality and plankton communities in experimental ponds. Graduate Research Assistant – Texas Christian University May - September 1997 Effects of bluegill (Lepomis macrochirus) on plankton, benthic macroinvertebrates and nutrients in the presence and absence of other fish species. Funded by an Adkins Graduate Student Fellowship. Undergraduate Research Project – Austin College January - May 1996 Effects of suspended clay on zooplankton reproduction and zooplankton community structure. Intern – City of Sherman Water Monitoring Lab (Sherman, Texas) January - February 1996 Analysis of samples of local industry effluents into the city waste water treatment system and samples from within the waste water treatment plant. Undergraduate Research Assistant – Texas Christian University May - September 1995 Development of electrofishing model for the estimation of largemouth bass densities. January Term in Ecuador – Austin College January 1995 Botanical and natural history field work in tropical mountain and rainforest ecosystems. Weston Hugh Nowlin – Curriculum Vitae 19 Teaching Experience Texas State University, Department of Biology September 2005 – Present - Wetlands Ecology (BIO 7422) - Community and Ecosystem Ecology (BIO 7302) - General Ecology (BIO 4416) - Undergraduate Research (BIO 4299) - Graduate Research (BIO 5214) - Seminar in Aquatic Resources (BIO 7102) Miami University, Department of Zoology September – December 2004 Postdoctoral Lecturer - Fundamentals of Ecology (ZOO 204) University of Victoria, Department of Biology September 1999 - September 2002 Departmental Teaching Assistantship (Laboratories) - Principles of Ecology (BIOL 215) - Survey of Invertebrates (BIOL 321) - General Biology (BIOL 190B) - General Biology (BIOL 110) Mansfield High School, Mansfield, Texas January - May 1999 Science Teacher - Biology - Integrated Physics and Chemistry Texas Christian University, Department of Biology January 1997 - December 1998 Departmental Teaching Assistantship (Laboratories) - Principles of Life Science for Majors (BIOL 105) - Principles of Life Science for Non-Majors (BIOL 100) Weston Hugh Nowlin – Curriculum Vitae 20 Jean K. Krejca, Ph.D. President General Qualifications Dr. Krejca has extensive experience with the identification and regulation of endangered species and habitat in Texas. She is a specialist in the biology of subterranean species including karst invertebrates, bats, and Eurycea salamanders. Additionally she has widespread experience with freshwater mussel species. She has been responsible for the design, implementation, and management of time sensitive environmental projects dealing with karst and aquatic issues in Bexar, Travis, Williamson, and Hays counties in central Texas. Dr. Krejca’s project experience also includes leading biological inventories, rare and endangered species surveys, and developing customized biological studies. She has conducted karst feature surveys on 5000 acres in central Texas since 2000. Her clients are public and private sector organizations and developers, and she has consulted numerous times with USFWS to obtain an Incidental Take Permit (section 10(a)(1)(B)), or to perform avoidance measures and therefore guide the client to an alternative to a take permit. She has received numerous federal and state grants for cutting edge research, and her research has been published in peer-reviewed journals. With Zara Since: 2003 Industry Experience Since: 1990 Education Ph.D., 2005, Ecology, Evolution, and Behavior, University of Texas B.S., 1995, Zoology, Southern Illinois University at Carbondale Certifications USFWS Endangered Species 10(a)(1)(A) Permit TE85077A Diver: Advanced Open Water and Full Cave certified CPR, First Aid, cave rescue OSHA confined space TxDOT Pre-Certifications 2.6.1 Protected Species Determination (Habitat) 2.6.2 Impact Evaluation Assessments 2.6.3 Biological Surveys Experience Deep Aquifer Biota Study, Bexar, Comal and Medina counties, Texas (2007 ongoing as of 2013). Edwards Aquifer Authority. The Edwards Aquifer contains a diverse array of stygobitic fauna that is rarely accessible for scientific research. This project has offered an opportunity to sample large quantities of well outflows through a unique agreement with land owners. Beginning in 2008, we built and installed infrastructure that allowed us to sample well sites that are inaccessible without major modification. The sampling of wells has led us to the documentation of at least six new locality records for crustaceans, added an entire order of fauna previously undocumented from the Edwards Aquifer, facilitated the collection of a species of copepod previously unknown to science, (Diacyclops sp.), and documented the persistence of Trogloglanis pattersoni and Satan eurystomus, two species of blind catfishes that have been largely absent from the scientific literature since 1978. Prior to this study, no genetic information was available for either of these two species of blind catfishes, however this sampling has allowed material from both species of blindcat to be collected and genetically sequenced for the first time. Intrepid Potash Karst Invertebrate Technical Report, Eddy County, New Mexico (October 2011 - August 2012). Intrepid Potash. We conducted inventories of terrestrial and aquatic subterranean fauna in three gypsum caves in support of an Environmental Impact Statement for a proposed potash mining project in Eddy County, New Mexico. All three caves showed a broad array of terrestrial invertebrate fauna, including typical cave fauna Zara 1 such as Ceuthophilus crickets, Rhadine ground beetles, Cicurina spiders, and Speorthus millipedes. Groundwater sampling for fauna revealed an undescribed species of Parabogidiella amphipod known only from Skylite Cave, and an undescribed species of Diacyclops copepod known only from Selenite Moon and Skylight caves, as well as two species of ostracods. Terrestrial fauna include an undescribed species of Rhadine beetle that is also known from gypsum caves nearby. Barton Springs Environmental Assessment, Travis County, Texas (2011-2012). City of Austin (via subcontract through Weston Solutions Inc.). Zara Environmental is assisting in the development of an Environmental Assessment (EA) for the renewal of a Section 10(a)(1)(B) permit and Habitat Conservation Plan for the Barton Springs Salamander, Eurycea sosorum and the Austin Blind Salamander, Eurycea waterlooensis. Proposed mitigation and minimization activities associated with the operation and maintenance of Barton Springs Pool include management of a refugium population, habitat restoration, scientific studies on both species and their habitats, and education and outreach about the spring complex and the Edwards Aquifer. Biological Surveys at Jacob’s Well, Hays County, Texas (2009-2012). The Meadows Center for Water and the Environment at Texas State University (formerly River Systems Institute. Zara was responsible for performing a complete biological inventory and updating the subterranean map of Jacob’s Well Cave using SCUBA techniques. This project yielded specimens of an undescribed (i.e.-new to science) amphipod species, and collections of Eurycea salamanders from deep within the well. The conclusion of this project will include genetic sequencing of the salamanders, a description of the amphipod, and will contribute to a greater understanding of the surface and subsurface aquatic communities at Jacob’s Well Spring. Edwards Aquifer Recovery Implementation Program (EARIP) Habitat Conservation Plan (HCP) and Environmental Impact Statement (EIS) (2009-2012). RECON Environmental, Inc. for the Edwards Aquifer Recovery Implementation Program. Zara biologists and hydrogeologists assisted in the preparation of the Environmental Settings and Baseline chapters of the EARIP HCP and EIS documents. Specific duties included review of previous reports, technical writing, quality assurance/quality control (QA/QC) of documents, meeting attendance and presentation to stakeholers, and consultation with the USFWS. Stakeholders included the Edwards Aquifer Authority (EAA) Board of Directors, the City of San Marcos City Council, the City of New Braunfels City Council, the San Antonio Water System Board of Directors, and the office of the President of Texas State University. The EARIP is intended to resolve the longstanding conflict between the federal mandate to protect threatened and endangered species associated with the Edwards Aquifer and the region's dependence on the same aquifer as its primary water resource. Stakeholders received an Incidental Take Permit (ITP) under Section 10(a)(1)(B) of the Endangered Species Act (ESA). The ITP allows for the “incidental take” of threatened or endangered species resulting from the otherwise lawful activities involving regulating and pumping of groundwater from the Edwards Aquifer. The HCP and EIS supported the issuance of an ITP which Eight species that depend directly on water in or discharged from the Aquifer are federally-listed as threatened or endangered. These species include fountain darter (Etheostoma fonticola), San Marcos salamander (Eurycea nana), San Marcos gambusia (Gambusia georgei), Texas blind salamander (Eurycea [formerly Typhlomolge] rathbuni), Peck's cave amphipod (Stygobromus pecki), Comal Springs dryopid beetle (Stygoparnus comalensis), Comal Springs riffle beetle (Heterelmis comalensis), and Texas wild rice (Zizania texana). The primary threat to these Aquifer-dependent species is the intermittent loss of habitat from reduced springflows. The HCP and EIS included the entire Edwards Aquifer Authority Jurisdictional Area of Bexar, Caldwell, Comal, Guadalupe, Hays, Medina, and Uvalde counties, Texas. USFWS issued its ROD and IPT on 18 March 2013. Zara Jean K. Krejca, Ph.D. 2 Hays County Regional Habitat Conservation Plan Consultation and Co-Author, Hays County, Texas (2008-2010). Bowman Consulting Group, Ltd. (formerly Loomis Partners) for Hays Count. Consultant and coauthor of Hays County Regional Habitat Conservation Plan. Responsibilities included technical writing for the regional habitat conservation plan (RHCP) and environmental impact statement (EIS), document review, meeting attendance and presentation to County Commissioners and technical and citizen groups, and consultation with the U.S. Fish and Wildlife Service (USFWS). The Hays County RHCP includes conservation measures to minimize and mitigate incidental take of the golden-cheeked warbler and black-capped vireo and establishes a preserve system of 10,000-15,000 acres to mitigate for the incidental take. The RHCP and EIS were approved by USFWS 13 May 2011. This plan also included consideration of rare, non-listed karst invertebrate species. To help the County in selecting conservation lands for the plan, Zara Environmental assisted them in obtaining a USFWS/TPWD Section 6 grant to study the distribution of rare species and to establish karst fauna regions. Detectability of karst invertebrates (2006 to 2007). Texas Parks and Wildlife Department. Zara designed, performed, and authored a year-long study analyzing large datasets of karst species abundance. Species detections at 23 caves each surveyed eleven times were analyzed in relation to eleven environmental covariates thought to impact the detectability of karst species. The covariates were not found to affect the detectability of the species, and further analyses demonstrated the number of surveys needed to achieve a 95% confidence level for proving species absence. Grant awarded through U.S. Fish and Wildlife Service Section 6 program. Mark-Recapture Study, Hays County, Texas (2005-2007). Texas Parks and Wildlife Department. A markrecapture study of Eurycea rathbuni at two caves in San Marcos, Texas. Declines in this rare species have been noted in the past, and this study provided data-backed population estimates for these locales. Grant awarded through U.S. Fish and Wildlife Service Section 6 program. Zara Jean K. Krejca, Ph.D. 3 Krista M. McDermid Biologist/Project Manager General Qualifications Krista holds a Master’s degree in Wildlife Ecology from Texas State University in San Marcos and a bachelor’s degree in Evolution, Ecology and Behavior from The University of Texas at Austin, where she worked on behavioral and genetic development of the zebra fish, Danio rerio. Before joining Zara, Krista worked as a biologist for the Texas Commission on Environmental Quality, Texas Parks and Wildlife Department, the City of Austin. Since 2007 she has participated in numerous projects including surveys for state and federally listed karst invertebrates, mammals, birds, aquifer fauna and freshwater mussels. She has conducted karst feature surveys on more than 500 acres in central Texas since 2007. She has also conducted aquatic macro-invertebrate habitat and presence/absence surveys for aquifer species in Hays, Bexar, Uvalde and Medina Counties. With Zara Since:2007 Industry Experience Since: 2005 Education M.S., 2009, Wildlife Ecology, Texas State University Certification, 2010, G.I.S., Pennsylvania State University B.S., 2004, Biology, University of Texas Certifications USFWS Endangered Species 10(a)(1)(A) Permit TE85077A TPWD Scientific Research Permit SPR-0608-082 SCUBA, open-water and advanced CPR, First Aid, Cave Rescue In addition to the project-specific experience outlined OSHA Confined Space below, Krista has experience with the development and implementation of Habitat Conservation Plans, Environmental Impact Statements, Environmental Assessments, Biological Assessments, and Section 7 consultations. Selected Experience US 281 Environmental Impact Statement (2009 to present), San Antonio, Bexar County, Texas. Alamo Regional Mobility Authority (via Jacobs Engineering). Assistant Project Manager. Zara performed field work and writing for a large scale EIS covering roadway through potential endangered species habitat and sensitive aquifer recharge zones. Field work included endangered species surveys for karst invertebrates and a geological assessment. Writing included assisting with the draft and final EIS and with the NEPA process (e.g. public meetings, USFWS consultation). Additionally, Zara staff provided guidance and consultations to engineers, lawyers, Texas Department of Transportation and Federal Highway Administration for avoiding and mitigating environmental impacts. The level of support Zara is provided includes litigation support for a process that is expected to come under intense scrutiny. Edwards Aquifer Well and Cave Sampling for Aquifer Biota: Comal, Bexar, Medina and Uvalde Counties, Texas (2008 to present). Edwards Aquifer Authority. Project Manager, Scientist. Responsibilities include project design, management oversight, right of access coordination with landowners, field collections and assessments, and extensive lab and field work with the aquatic Zara 1 fauna of the Edwards Aquifer. This project includes habitat surveys and presence/absence surveys at more than twelve sites with the potential to contain listed aquatic macro-invertebrate and two fish species that have been proposed for listing. Lakeline Mall Habitat Conservation Plan, Travis and Williamson Counties, Texas (2007 to present). Texas Cave Management Association. Project Manager. Responsibilities include project management oversight and field work. Zara has an ongoing contract to survey mitigation preserves for listed karst species. This includes evaluating presence/absence of karst invertebrates including arachnids and insects, and monitoring subterranean salamander populations multiple times per calendar year. Decker Lake Power Plant Ichthyoplankton Entrainment Study, Section 316b, Austin, Texas (2013). AMEC. Project Manager. Conducted aquatic vegetation surveys and monthly 24-h entrainment sampling events at Decker Lake Power Plant, Austin, Texas, in accordance with activities related to Section 316b of the Clean Water Act. Deer Lane Groundwater Study (2011 to 2012). City of Austin. Scientist. Zara was selected by the Austin City Council to be the sole-source recipient of a contract to conduct tracer tests along Deer Lane in south Austin. The project goal is ensure that this road improvement project will be in compliance with permitting requirements related to the Balcones Canyonlands Conservation Plan agreement with USFWS. Zara’s role is to conduct background water quality monitoring, groundwater tracer tests, and fauna surveys in three caves near the project. The tracer tests will use safe dyes and chemical tracers to simulate how contaminants from roadway runoff might move through the soils and into cave drips and the aquifer. The results of the study will help engineers implement water quality measures to ensure that impacts to the caves, cave fauna, and groundwater are minimized. Environmental Assessment services and Easement Documentation Report preparation for Proposition 1 and 3 lands near Government Canyon State Natural Area, Bexar and Medina Counties, Texas (2011). Texas Parks and Wildlife Department. Scientist. Zara biologists conducted all field work necessary to document the condition of approximately 3,000 acres of City of San Antonio (COSA) Proposition 1 and 3 Edwards Aquifer Program Natural Areas and prepare the Easement Documentation Report (EDR) for use in the development of a conservation easement between the COSA and Texas Parks and Wildlife Department (TPWD). The goal of the easement agreement is to preserve groundwater recharge water quality and assist in mitigation of endangered Golden-cheeked Warbler habitat for Camp Bullis (United States Army medical training facility) training area expansion. Karst Habitat Determination at Medina Dam, Medina County, Texas (2011). San Antonio River Authority (via URS Corporation). Project Manager. A permitted biologist from Zara Environmental evaluated all reinforcing boreholes along the east wing wall of Medina Lake Dam to determine if they contained voids with potential karst invertebrate habitat. Zara Krista M. McDermid 2 Edwards Aquifer Recovery Implementation Program (EARIP) Habitat Conservation Plan (HCP) and Environmental Impact Statement (EIS) (2009 to 2012). RECON Environmental, Inc. for the Edwards Aquifer Recovery Implementation Program. Scientist. Assisted in the preparation of the Environmental Settings and Baseline chapters of the EARIP HCP and EIS documents. Specific duties included review of previous reports, technical writing, quality assurance/quality control (QA/QC) of documents, meeting attendance and presentation to stakeholers, and consultation with the USFWS. The HCP and EIS supported the issuance of an ITP which eight species that depend directly on water in or discharged from the Aquifer are federally-listed as threatened or endangered. These species include fountain darter (Etheostoma fonticola), San Marcos salamander (Eurycea nana), San Marcos gambusia (Gambusia georgei), Texas blind salamander (Eurycea [formerly Typhlomolge] rathbuni), Peck's cave amphipod (Stygobromus pecki), Comal Springs dryopid beetle (Stygoparnus comalensis), Comal Springs riffle beetle (Heterelmis comalensis), and Texas wild rice (Zizania texana). The HCP and EIS included the entire Edwards Aquifer Authority Jurisdictional Area of Bexar, Caldwell, Comal, Guadalupe, Hays, Medina, and Uvalde counties, Texas. Fauna inventory of Jacob’s Well, Hays County, Texas (2009 to 2012). River Systems Institute. Project Manager. Responsibilities include project design and execution for a complete survey of Jacob’s Well, an underwater cave in Wimberley, Texas, and the surrounding stream habitat. This includes collection and identification of aquifer adapted and stream dwelling aquatic invertebrates and salamanders. This site may have the potential to contain listed aquatic macro-invertebrate or undescribed species. Karst Fauna Mapping, Hays County, Texas (2009). Hays County. Scientist. Responsibilities include field work for the evaluation of Hays County caves for the presence of karst invertebrates and karst invertebrate collection management. This project involves the identification and collection of arachnid and insect species that have the potential to become listed species based on their narrow habitat parameters. Jollyville Transmission Main, Austin, Travis County, Texas (2009 to 2011). Hicks and Co. (Black and Veatch Engineering, City of Austin). Scientist. Zara performed karst species consultation services including spatial analysis of environmentally sensitive areas, research on localities and potential impacts on the Jollyville Plateau Salamander and endangered karst invertebrates, karst feature surveys, and USFWS consultations. Consultations with the City of Austin and USFWS included navigation of multiple HCP’s, including the Comanche Canyon HCP and the Travis County Regional HCP. Camp Bullis Karst Research, San Antonio, Bexar County, Texas (2007 to 2012). Department of Defense (via subcontract through Prewitt and Associates for years 2003 to 2009). Scientist (20072009); Project Manager (2009-2011). Zara Environmental is now the prime contractor for this project. This project is a series of year-long contracts each covering a myriad of sub-projects. Responsibilities have varied each year, but include project management, design, and execution. Examples of projects include: performing karst surveys, monitoring endangered species, evaluating karst invertebrate habitat, performing spring surveys (biota and water parameters), identifying species, excavating caves, building cave gates, evaluating hydrogeology of caves and features, Zara Krista M. McDermid 3 executing dye traces, analyzing rainfall data, analyzing the flow of shallow groundwater over endangered species localities in relation to cedar, clearing cedar, and performing custom research projects related to Eurycea salamander distribution, abundance, and genetics. Bat and Endangered Species Monitoring, San Antonio, Bexar County, Texas (2007 to 2009). Department of Defense (subcontracted through Texas Cave Management Association). Scientist. Endangered species and bat habitat monitoring, hydrogeologic analysis, and control of red imported fire ants at cave and karst features on Camp Bullis military installation. Selected Publications and Presentations Krejca, J., Clamons, C., Cowan, B., McDermid, K., and A. Oliver. 2013. State-listed Threatened Mussels and Invasive Zebra Mussels: What You Need to Know to Avoid Project Delays. Poster presentation for Texas Water 2013 Conference. Galveston, Texas. 10-11 April 2013. McDermid, Krista, Krejca, J., Clamons, C. Cowan, B. and A. Oliver. 2013. Survey methods and freshwater mussel distribution by depth in the Trinity River. Poster presentation for the Freshwater Mollusk Conservation Society 2013 Symposium. Guntersville, Alabama. McDermid, Krista, Krejca, J., Clamons, C. Cowan, B. and A. Oliver. 2013. Unexpected Diversity and Abundance in Highly Impacted Segments of the Trinity River. Poster presentation for the Freshwater Mollusk Conservation Society 2013 Symposium. Guntersville, Alabama. McDermid, Krista. 2012. Bats in Texas Bridges. Presented at Bat Conservation International's Batfest Symposium, San Antonio, Texas. Weckerly, Floyd and Krista McDermid. 2010. Addendum-Karst invertebrate species survey protocol and trend analyses at Balcones Canyonlands Preserve. Report prepared for the City of Austin. McDermid, Krista. 2009. Plethedon albagula diet. Herpetological Review 40: 197. McDermid, Krista and Andrew Gluesenkamp. 2009. Salamanders of the genus Eurycea at Camp Bullis, Bexar County, Texas. Proceedings of the 15th International Congress of Speleology, Kerville, Texas. McDermid, Krista. 2009. Food habits of the common musk turtle (Sternotherus odoratus) in lentic and lotic habitats, Spring Lake, Hays County, Texas. MS thesis: Texas State University-San Marcos. 47 pp. Fotinos, Timothy A., Andrew Blair, S. Ben Harnden, Krista McDermid, and Arjun Adhikari. 2007. Small-scale patterns of plant species richness: implications for biodiversity and exotic invasion. Southwestern Association of Naturalists (SWAN) Poster Presentation. Zara Krista M. McDermid 4 Doyle CV- 2012 Page 1 of 16 Robert D. Doyle, Ph.D. Baylor University, Department of Biology PO Box 97388, Waco, TX 76798 254-710-2911, [email protected] Update 6/2012 PART I. BASIC INFORMATION EDUCATION: 1991 Ph.D. Marine, Estuarine, and Environmental Sciences: University of Maryland. Dissertation Title: Primary Production and Nitrogen Cycling Within the Periphyton Community Associated with Emergent Aquatic Macrophytes in an Amazon Floodplain Lake. 1985 M.S. Biology: Baylor University Thesis Title: Phytoplankton Production of Tropical Lake Chapala, Mexico. 1981 B.S. Biology: Baylor University, Waco, TX. PROFESSIONAL EXPERIENCE: 2005-present Chair, Department of Biology, Baylor University 2003-present Director, Center for Reservoir and Aquatic Systems Research (CRASR) 2008-present Professor, Baylor University 2001-2008 Associate Professor, Baylor University 1998-2001 Assistant Professor, University of North Texas. 1996-1998 Research Biologist, US Army Corps of Engineers, Waterways Experiment Station, Lewisville Aquatic Ecosystem Research Facility, Lewisville TX. 1991-1998 Adjunct Assistant Professor, Un of North Texas, Department of Biological Sciences 1993-1996. Research Scientist II, University of North Texas, Institute of Applied Sciences. 1991-1993 Research Scientist I, University of North Texas, Institute of Applied Sciences. 1986-1991 Graduate Research Assistant, University of Maryland, Marine, Estuarine, Environmental Science Program. . 1985-1986 Research Associate, University of Maryland, Amazon Floodplain Research Project. CERTIFICATION: 1995- present Professional Wetland Scientist, Society of Wetland Scientists HONORS AND AWARDS: 2003-present Fellow, Texas Academy of Sciences 1997, 1998 Department of the Army, Official Commendation for exceptional performance of duties as Research Biologist. 1995 UACE Wetlands Research Program, Commendation for contribution to the WRP, 1981 Recipient of the Cornelia M. Smith Award for Outstanding Biology Senior at Baylor University. 1981 Graduated cum laude from Baylor University. Doyle CV- 2012 Page 2 of 16 PART II. RESEARCH AND PUBLICATIONS GRANTS & CONTRACTS Current Direct Funding (Doyle as PI or co-PI) Gear-Up Waco: Marsh Madness. Principal investigator of the “Marsh Madness: environmental education module of the overall Gear-Up Waco program, 2006-2012, $1.16M total over 6 years. Total Gear-Up Waco award of $12.2M over 6 years to Baylor University (various PI’s). Previous (completed) funding at Baylor Restoration of the lower San Marcos River, TX. (Cooperative agreement with the USFWS, 10/20057/2011, $76,000). R. Doyle is the principal investigator of this project to develop methods for largescale native aquatic plant restoration of the lower San Marcos river following dredging efforts to remove a nuisance exotic invasive plant species (Cryptocoryne beckettii). Coastal Prairie Freshwater Wetland Function Project, (TCEQ & Galveston Bay Estuary Program, 2007-2009, $250,000; 2009-2010 $111,570, total project = $361,570). R. Doyle and M. Forbes (coprincipal investigators). Dr. Forbes is a post-doc in my lab and has developed this funding to develop water quality and flood storage functional assessment models for geographically isolated depressional wetlands along the Texas coastal prairie ecosystem near Houston, TX. Lake Whitney Comprehensive Assessment, (Project Director for comprehensive evaluation of Lake Whitney for possible use as a drinking water source. Numerous scientists from Baylor’s departments of Biology, Geology, Environmental Studies and the Center for Spatial Research are involved, 2006-2008). Phase 1a: Physical Limnology (USEPA, $396,000) Phase 1b: Biological Assessment (DOE, $481,000) Phase 1c: Ambient Toxicity and Contaminant Assessment (USEPA, $197,000) Transition Zone Index Development and Validation Project (2004-2008, $615,000 4-year total, funding from TCEQ via BRA- co principal investigator with Bryan Brooks). This grant focuses on developing criteria for identifying and modeling the “transition area” of a reservoir between the inflowing stream and the lake-like lacustrine zone. Improving Ammonia and Phosphorus Removal in Subsurface Flow Wetlands, (TCEQ- Texas Onsite Wastewater Treatment Research Council, 2007-2008, $107,000, collaborator with Joe Yelderman). This project uses pilot scale subsurface flow (SSF) wetlands and soil columns to conduct research that will lead to improvements in nutrient removal from septic tank effluent. Lake Waco Study, 2002-2005: ($1.1 million total to BU, $152,324 for Doyle, City of Waco via ENSR. Doyle served as overall project manager for Baylor’s funding). Lake Waco Nitrogen Fixation (FY 02, 03 & 04 total = $66,337). Lake Waco Monitoring (FY 03, 04 & 05 total = $85,987). Spatial Patterns in Wetland Nutrient Biogeochemistry: Implications for Ecosystem Functions. Texas Agriculture Extension Station, Water Resources Institute, $5,000. J.T. Scott & R. Doyle. Baylor Experimental Aquatic Research support grant. Altria Group, Inc. – 2004 Environment/Water Conservation. $35,000. 2005-2006. R. S. King, B. W. Brooks, R. D. Doyle. Lake Cypress Springs Herbivorous Insect Evaluation ($6,000 Franklin County, TX) Enhancement of 3km Segment of San Marcos- 2003-04 ($24,284, USFWS Section 6 via TPWD) Doyle CV- 2012 Page 3 of 16 This grant provides partial support for our second year restoration efforts on the San Marcos River, TX. which includes efforts to eradicate an exotic aquatic plant and re-establish desirable native vegetation. Cryptocoryne beckettii control in the San Marcos River- Phase I, 2002-2003. ($25,000USFWS). A cooperative agreement has been established with the USFWS for on-going research and management efforts on the San Marcos River. The first phase of this cooperative agreement was funded at $25,000. This funding continued a fruitful and long-term cooperative agreement. In the past the USFWS has funded me for about $350,000 of research on the San Marcos. Additional funds followed this grant (see above). Evaluation of the peroxidase enzyme assay with respect to insect herbivory on Hydrilla verticillata (2001-02, $5,825-Baylor URC). 1998-2001 Funding while a tenure-track faculty at UNT Impacts of Hydrellia pakistanae on plant competitive interactions -COE Waterways Experiment Station $20,000. Impacts of Hydrellia pakistanae on Hydrilla verticillata -COE Waterways Experiment Station $33,081. Assessment of Factors Influencing Texas Wildrice (Zizania Texana) Sexual and Asexual Reproduction , Edwards Aquifer Authority (Co-PI with P. Power for grant received by USFWS-NFH), $63,060. Development of Water Quality Monitoring System for Lake Texoma, Co-investigator with Dickson, Waller, Atkinson - US Army Corps of Engineers via Wendy Lopez and Associates- $299,059. Inter-personnel Act Agreement-US Army Corps of Engineers Waterways Experiment Station- Summer salary support for Robert Doyle 1999 & 2000, $ 39,296. Inter-personnel Act Agreements- US Army Corps of Engineers Waterways Experiment Station Salary support for Dr. Gary Dick 10/98-9/00, $119,750. Inter-personnel Act Agreements- US Army Corps of Engineers Waterways Experiment Station Salary support for Mr. Matt Francis 2/00-12/00, $31,334.95. Inter-personnel Act Agreements- US Army Corps of Engineers Waterways Experiment Station 50% salary support for Mr. David Honnell 6/99-5/01, $61,836. Establishment of native aquatic plans for lake restoration and fish habitat enhancement- Texas Parks and Wildlife Department, Co-investigator with Dickson and Dick, $137,684. Development of Aquatic Ecosystem Restoration Technologies- Aquatic Ecosystem Restoration Foundation, $36,500. Determination of the carbon sources for Texas Wildrice and other aquatic plants of the Comal and San Marcos Rivers, UNT Faculty Development Grant, $3,800. 1992-1998 Funding (Prior to joining faculty at UNT in 1998) 1998, Effects of Biocontrol Agents on the Competitive Interactions Between Exotic and Native Plant Species (COE, 70,000, Co-PI w/ M. Smart) 1998, Assessment of Vegetation Loss During Low Flows for Development of Adaptive Management Recommendations (USFWS, $101,000) 1998, Restoration of TX Wildrice and Other Vegetation: Comal and San Marcos Rivers, (USFWS, $14,333) 1998, Effects of Inorganic Clay Turbidity on the Growth and Reproductive Potential of Native Submersed Plants (COE, $12,500) 1998, Lake Wister Native Plant Establishment (Tulsa COE, $13,250) 1998, Eradication of Hydrilla on the Comal River and Restoration with Native Species (USFWS, $6,000) 1998, Development of a Wetland For Water Quality Benefits of Oyster Creek, TX. (Brazos River Authority, $15,000) Doyle CV- 2012 Page 4 of 16 1997, Assessment of Vegetation Loss During Low Flows for Development of Adaptive Management Recommendations (USFWS , $101,000) 1997, Restoration of TX Wildrice and Other Vegetation: Comal and San Marcos Rivers (USFWS , $14,333) 1997, Effects of Inorganic Clay Turbidity on the Growth and Reproductive Potential of Native Submersed Plants (COE, $54,000) 1997, Survival of Macrophytes Under Simulated Wave Energy Regimes (COE, $35,000) 1997, Write section of Environmental Manual entitled ALimnology 101" (COE, $12,000) 1996, Establishment of Native Aquatic Plants in Lake Livingston: Stage I & II, (Texas Parks and Wildlife Department, $30,000 Co-PI w/ M. Smart) 1995-1996, Plantings of Beneficial Native Aquatic Plants in Eagle Mountain Lake, TX. (Tarrant County Water Control District, $7,900, Co-PI with M. Smart) 1995-1996, Effects of Inorganic Clay Turbidity on the Growth and Reproductive Potential of Native Submersed Plants, (COE, $150,000) 1995-1998, Mitigation for Environmental Damages to the Lewisville Lake Environmental Learning Area (Upper Trinity Region Water District, $200,000. Doyle’s role: Negotiated settlement for LLELA with UTRWD) 1995-1997, Establishment of Native Aquatic Macrophytes in Lake Conroe for Fish Habitat. (Lake Conroe Restocking Association, $50,000. Co-PI w/ Mike Smart) 1993-1994, Fate of Atrazine in Wetland Mesocosms. (COE Wetlands Research Program, $130,000, CoPI w/ Mike Smart) 1993, Development of Wetland Trail at the Lewisville Lake Environmental Learning Area, (EPA Region 6, $20,000) 1992-1993, Planting Developed Wetlands at Lake Ray Roberts, TX. (EPA $20,000, Co-PI w/ M. Smart) PUBLICATIONS: Peer-Reviewed Publications: The following articles have all been published in (or submitted to) international journals. 38. Clap, AG, MG Forbes, RD Doyle, JC Yelderman. (in review, Wetlands). Hydrology of coastal prairie wetlands of the Upper Gulf Coast, Texas. 38. Heimbach, E. et. al. 2012 accepted for publication. The Effects of Lowering Uric Acid Levels Using Allopurinol on Components of Metabolic Syndrome. Cardiology Research. 37. Forbes, MG, J Back, RD Doyle. (2012 accepted for publication). Nutrient transformation and retention by coastal prairie wetlands, Upper Gulf Coast, Texas. Wetlands. 36. Hubbs, A, EI Doyle, RG Bowden, RD Doyle. 2012 accepted for publication. A multiple variable health assessment and descriptive study among college students. Psychological Reports. 35. Forbes, MG, RD Doyle, JT Scott, J Stanley, H Huang, BA Fulton, and BW Brooks. 2012. Carbon sink to source: Longitudinal gradients of planktonic P:R ratios in subtropical reservoirs. Biogeochemistry. 107:81-93. 34. Enwright, N, MG Forbes, RD Doyle, B Hunter, and W Forbes. 2011. Using Geographic Information Systems to Inventory Coastal Prairie Wetlands along the Upper Gulf Coast, Texas. Wetlands. 31:687-697. 33. Forbes, MG, JC Yelderman Jr, T Potterton, RD Doyle. 2010. Effects of intermittent loading, media type, and wetland age on nitrogen removal in horizontal subsurface flow wetlands. Water Science and Technology 62:1865-1871. 32. Doyle, RD, JT Scott, M Forbes. 2010. Hot Spots and Hot Moments of Planktonic Nitrogen Fixation in a Eutrophic Southern Reservoir. Lake and Reservoir Management 26:95-103. Nominated for Society of Wetland Scientist “Labounty Award for Best Paper” 2010. 31. Scott, JT, JK Stanley, RD Doyle, M Forbes, BW Brooks, 2009. River-reservoir transition zones are nitrogen fixation hotspots regardless of ecosystem trophic state. Hydrobiologia, 625:61-68 30. Scott, JT, DA Lang, RS King, RD Doyle, 2009. Nitrogen fixation and phosphatase activity in Doyle CV- 2012 Page 5 of 16 periphyton growing on nutrient diffusing substrata: evidence for differential nutrient limitation in stream periphyton. J. North American Benthological Association. 28(1):57-68. 29. Forbes, MG, RD Doyle, JT Scott, J Stanley, H Huang, BW Brooks, 2008. Physical factors control carbon and nitrogen fixation in eight Texas reservoirs. Ecosystems. 11:1181-1197. 28. Alexander, M, RD Doyle, P Power. 2008. Suction Dredge Removal of an Invasive Macrophyte From a Spring-fed River in Central Texas, USA. J. Aquatic Plant Management. 46:184-185. 27. Scott, JT, RD Doyle, S Prochnow, and JD White. 2008. Are watershed and lacustrine controls on planktonic n2 fixation hierarchically structured?. Ecological Applications. 18:805-819. 26. Engle, DL, JM Melack, RD Doyle, and TR Fisher, 2008. High rates of net primary production and turnover of floating grasses on the Amazon floodplain: Implications for aquatic respiration and regional CO2 flux. Global Change Biology 14:396-381. 25. Scott, J.T., M.J. McCarthy, W.S. Gardner, and R.D. Doyle. 2008. Denitrification, dissimilatory nitrate reduction to ammonium, and nitrogen fixation along a nitrate concentration gradient in a created freshwater wetland. Biogeochemistry 87:99-111. Doyle, R.D., M. Grodowitz, M. Smart, and C. Owens, 2007, Separate and interactive affects of competition and herbivory on the growth, expansion and tuber formation of Hydrilla verticillata. Biological Control 41:327-338. 24. Scott, J.T., R.D. Doyle, J.A. Back, and S.I. Dworkin. 2007. The role of N2 fixation in alleviating N limitation in wetland metaphyton: enzymatic, isotopic, and elemental evidence. Biogeochemistry 84: 207-218. 23. Scott, J.T., R.D. Doyle. 2006. Coupled photosynthesis and heterotrophic bacterial biomass production in a nutrient-limited wetland periphyton mat. Aquatic Microbial Ecology 45:69-77. 22. Forbes, M., K. Dickson, F. Saleh, W. Waller, R.D. Doyle, and P. Hudak. 2005, Recovery and fractionation of phosphorus retained by lightweight expanded shale and masonry sand used as media in subsurface flow treatment wetlands. Environmental Science and Technology 39:46214627. 21. Scott, J.T., R.D. Doyle, and C.T. Filstrup. 2005, Periphyton nutrient limitation and nitrogen fixation potential along a wetland nutrient depletion gradient. Wetlands 25:439-448. 20. Power P. and R.D. Doyle. 2004. Carbon use by the endangered Texas Wild Rice (Zizania texana, Poaceae). Sida, Contributions to Botany, 21:389-398 19. Forbes, M., K. Dickson, T. Golden, R.D. Doyle, P. Hudak. 2004. Dissolved Phosphorus Retention of Light-Weight Expanded Shale and Masonry Sand Used in Subsurface Flow Treatment Wetlands. Environ. Sci. Technol. 38,892-898. 18. Doyle, R.D., M Francis, and R.M. Smart. 2003. Competitive interactions between Ludwigia repens and Hygrophila polysperma, morphologically similar aquatic plant species. Aquatic Botany 77:223-234. 17. Doyle, R.D., M. Grodowitz, R.M. Smart, C. Owens. 2002. Impact Of Hydrellia Pakistanae (Diptera: Ephydridae) herbivory On growth and photosynthetic potential of Hydrilla verticillata. Biological Control 24:221-229. 16. Doyle, R.D. 2001. Expansion and distribution of the exotic aquatic plant Cryptocoryne beckettii in the San Marcos River, TX. Sida; contributions to botany 19:1027-1038. 15. Best, P.H., C.P. Buzzelli, S.M. Bartell, R.L. Wetzel, W.A. Boyd, R.D. Doyle, and K.R. Campbell. 2001. Hydrobiologia. Modeling submersed macrophyte growth in relation to underwater light climate: modeling approaches and application potential. Hydrobiologia 444:43-70. 14. Doyle, R.D. 2001. Effects of waves on the early growth of Vallisneria americana. Freshwater Biology 46:389-399. 13. Doyle, R.D. and R.M. Smart. 2001. Impacts of water column turbidity on the survival and growth of Vallisneria americana winterbuds and seedlings. Lake and Reservoir Management 17:17-28. 12. Doyle, R.D. and R.M. Smart. 2001. Effects of drawdown and dessication on tubers of Hydrilla verticillata, an exotic aquatic weed. Weed Science 49:135-140. 11. Doyle, R.D., P. Power, and K. Kennedy. 2000. Reintroduction potential of Texas Wildrice (Zizania Doyle CV- 2012 Page 6 of 16 texana) into the San Marcos River, Texas. pp 179-184, L.S. Williamson, LA Dlutkowski, and A.P. McCammon Soltis (eds), Proceedings, Wild Rice Research and Management Conference, Carlton, Minnesota. 10. Stewart, L.K., P.F. Hudak, and R.D. Doyle. 1998. Modeling hydrologic alterations to a developing wetland in an abandoned borrow pit. Journal of Environmental Management. 53:231-239. 9. Doyle, R.D. and R.M. Smart, 1998. Competitive reduction of noxious Lyngbya wollei mats by rooted aquatic plants. Aquatic Botany. 61:17-32. 8. Smart, R.M., G.O. Dick, and R.D. Doyle. 1998. Techniques for establishing native aquatic plants. J. Aquat. Plant Management. 36:44-49. 7. Doyle, R.D., R.M. Smart, C.Guest, and K. Bickel, 1997. Establishment of native aquatic plants for fish habitat: test plantings in two North Texas reservoirs. Lake and Reservoir Management.13:259-269. 6. Smart, R.M., R.D. Doyle, J.D. Madsen, and G.O. Dick. 1996. Establishment of native submersed aquatic plant communities for fish habitat. In L.E. Miranda and D.R. DeVries (eds.) Multidimensional Approaches to Reservoir Fisheries Management. American Fisheries Society Symposium, 16:347-356. 5. Doyle, R.D. and T.R. Fisher. 1994. Nitrogen fixation by periphyton and plankton on the Amazon floodplain at Lake Calado. Biogeochemistry 26:41-66. 4. Lind, O.T., R.D. Doyle, D.S. Vodopich, B.G. Trotter, J.G. Limon, L. Davalos-Lind. 1992. Clay turbidity; Regulation of phytoplankton production in a large, nutrient-rich tropical lake. Limnol. Oceanogr. 37(3): 549-565. 3. Limon, J.G., O.T. Lind, D.S. Vodopich, R.D. Doyle, and B.G. Trotter, 1989. Long- and short-term variation in the physical and chemical limnology of a large, shallow, turbid tropical lake (Lake Chapala, Mexico). Arch. Hydrobiol. /Suppl. 83 1:57-81. 2. Fisher, T.R., R.D. Doyle, and E.R. Peele. 1988. Size-fractionated uptake and regeneration of ammonium and phosphate in a tropical lake. Verh. Internat. Verein. Limnol. 23:637-641. 1. Davalos, L., O.T. Lind, and R.D. Doyle. 1989. Evaluation of phytoplankton-limiting factors in Lake Chapala, Mexico: Turbidity and the spatial and temporal variation in algal assay response. Lake and Reservoir Management 5:99-104. Reports and Other Publications (peer-reviewed reports are indicated) Forbes, MG., J Yelderman, RD Doyle, Adam Clapp, B Hunter and N Enwright. 2009. Hydrology of Coastal Prairie Freshwater Wetlands. Wetland Science and Practice. 62(3), 12-17. Doyle, R.D. et. al. 2009. Lake Whitney Comprehensive Water Quality Study, Final Report. Phase IA – Physical Limnology & Phase IC- Ambient Toxicity. Submitted to EPA, 447pp. Doyle, R.D. et. al. 2009. Lake Whitney Comprehensive Water Quality Assessment, Phase IB- Physical and Biological Assessment. Submitted to USDOE, 333 pp. Doyle, R.D. In Review. Development of Techniques for Establishment of Native Aquatic Vegetation in the Comal and San Marcos Rivers, TX. Final Report to USFWS (peer reviewed report). M. Grodowitz, R.D. Doyle, M. Smart, C. Owens. 2004. Hydrellia pakistanae and H. balciunasi- Insect biocontrol agents for hydrilla: boon or bust? (Peer-reviewed) Proceedings, XI International Symposium of Biological Control of Weeds in Canberra Australia. Doyle, R.D. 2001. Effects of inorganic clay turbidity on the growth and reproductive potential of submersed aquatic macrophytes. Technical Report, U.S. Army Engineer Waterways Experiment Station, Vicksburg, MS. (peer reviewed report) Doyle, R.D. 1999. Effects of Waves on the early growth of Vallisneria americana Upper Mississippi River- Illinois Waterway System Navigation Study. EVN Report 12 (peer reviewed report) Grodowitz, M., R. D. Doyle and Smart, R.M. 1999. Potential use of Insect Biocontrol Agents for Reducing the Competitive Ability of Hydrilla verticillata. Misc. Paper A-99-, November 1999. Doyle CV- 2012 Page 7 of 16 Smart, R.M., R.D. Doyle, Madsen, J.D., and Dick, G.O. 1996. Establishing native submersed aquatic plant communities in southern reservoirs. Technical Report A-96-2, U.S. Army Engineer Waterways Experiment Station, Vicksburg, MS. (peer reviewed report) Smart, R.M., G. Dick, and R.D. Doyle. 1996. Grass=Bass: Native Aquatic Plants for Fish Habitat (I). Our Inland Fisheries, Jan/Feb pp 20-24. Smart, R.M., G. Dick, and R.D. Doyle. 1996. Grass=Bass: Native Aquatic Plants for Fish Habitat (II). Our Inland Fisheries, March/April pp 70-76. Smart, R.M., G. Dick, and R.D. Doyle. 1996. Grass=Bass: But Hydrilla is not the solution to habitat needs. Our Inland Fisheries, May/June pp 71-75. Doyle, R.D. and R.M. Smart, 1995. Competitive interactions of native plants and nuisance species in Guntersville Reservoir. Proceedings, 29th Annual Meeting, Aquatic Plant Control Research Program. Miscellaneous Paper A-95-3, US Army Engineer Waterways Experiments Station, Vicksburg, MS, pp 237-242. Dickson, K., R.D. Doyle, and others. 1995. Ray Roberts Lake: Year Six Post-Impoundment Environmental Study. US Army Corp of Engineers Fort Worth District. 226p. Doyle R.D. and R.M. Smart. 1995. Potential use of native aquatic plants for long-term control of problem aquatic plants in Guntersville Reservoir, Alabama; Report 2, Competitive interactions between beneficial and nuisance species. Technical Report A-93-6, U.S. Army Engineer Waterways Experiment Station, Vicksburg, MS. (peer reviewed report) Smart, R. M. and R.D. Doyle, 1995. Ecological Theory and Management of Submersed Aquatic Plant Communities. Information Exchange Bulletin A-95-3, APCRP, US Army Engineer Waterways Experiment Station, Vicksburg, MS. (peer reviewed report) Doyle, R.D., and R.M. Smart. 1994. Guntersville reservoir plant competition studies. Proceedings, 28th Annual Meeting, Aquatic Plant Control Research Program. Miscellaneous Paper A-94-2, US Army Engineer Waterways Experiments Station, Vicksburg, MS, 138-143. Doyle, R.D. and R.M. Smart. 1993. Potential Use of Native Aquatic Plants for long-term Control of Problem Aquatic Plants in Guntersville Reservoir, Alabama. Report 1. Establishing Native Plants. Technical Report A-93-6., US Army Engineer Waterways Experiment Station, Vicksburg, MS, 66p. Doyle, R.D., and R.M. Smart. 1993. Competitive ability of selected aquatic macrophytes in Lyngbyadominated littoral zones of Guntersville Reservoir. Proceedings, 27th Annual Meeting, Aquatic Plant Control Research Program. Miscellaneous Paper A-93-2, US Army Engineer Waterways Experiments Station, Vicksburg, MS, 242-248. Doyle, R.D., K. Dickson, and D. Sturges. 1993. Created wetlands on Lake Ray Roberts, Texas: design, pre-impoundment status, and research opportunities. pp 827-834. Landin, M.C. (ed.) Wetlands: Proceedings of the 13th Annual Conference of the Society of Wetland Scientist, New Orleans, LA. South Central Chapter, Society of Wetland Scientist, Utica, MS. (peer reviewed paper in proceedings) Smart, R.M., R.D. Doyle, D. Murphy, and D. Webb. 1993. Establishment of floating-leaved and emergent wetland vegetation to restore degraded littoral environments. pp 663-667. Landin, M.C. (ed.) Wetlands: Proceedings of the 13th Annual Conference of the Society of Wetland Scientist, New Orleans, LA. South Central Chapter, Society of Wetland Scientist, Utica, MS. (peer reviewed paper in proceedings) Lind, O.T., R.D. Doyle, D.S. Vodopich, B.G. Trotter, G. Glass, L. Davalos-Lind, J.G. Limon. 1992. Lago de Chapala: factores que controlam la produccion de fitoplancton. Ingenieria Hidraulica en Mexico, 1:17-32. Fisher, T.R. and R.D. Doyle, 1987. Nutrient cycling in Chesapeake Bay. pps. 49-53 IN G.B. Mackiernan (ed).Dissolved Oxygen in the Chesapeake Bay: processes and effects. MD Sea Grant Pub. no. UM-SG-TS-87-03. (peer reviewed) Doyle CV- 2012 Page 8 of 16 PRESENTATIONS AT PROFESSIONAL CONFERENCES: Forbes, MG, J Back, RD Doyle, J Yelderman. 2012. Nutrient Processing Within Coastal Prairie Wetlands: A Nexus to Galveston Bay, TX. 9th INTECOL International Wetlands Conference, Orlando, Florida, USA. June 4-8, 2012, (platform presentation) Doyle, RD., M. Mullins, N. Schell, T. Conry. 2012. Marsh Madness: A Science Education Adventure Focused On Wetland Environments9th INTECOL International Wetlands Conference, Orlando, Florida, USA. June 4-8, 2012, (poster presentation) Doyle, RD. and M. Mullins. 2011. (invited presentation) An Ecologists View of the Pond. Texas Assoc. of Pond Societies (Dallas, TX Sept 23-25, 2011) Doyle, RD. and D. Barker. 2010. Biodegradeable Organic Carbon Inputs to Lake Waco, Texas. NALMS November 3-5, Oklahoma City. Doyle, RD. 2010. Nutrients in the aquatic environment: impacts to aquatic plants. The 2010 Biological Control for Nature Conference. Northampton, MA. (Oct 3-7). Invited symposium presentation. Doyle, RD., MG Forbes, JT Scott. 2010. Spatial patterns of planktonic production and respiration: a test of reservoir zonation theory. ASLO, June 6-11, Santa Fe, NM. Forbes, MG, RD Doyle, J Yelderman. 2010. Coastal Prairie Wetlands: Big benefits in small packages. Soc. Wetland Scientist, June 27-July 2, Salt Lake. Doyle, RD, JT Scott, M Mullins, N Shell, T Conry. 2009. Will the wetland save the lake? The lake waco wetland 2003-2009 experience. South-Central Chapter Society of Wetland Scientists, Denton, TX (invited plenary talk). Doyle, RD, MG Forbes, TJ Scott. 2009. Spatial patterns of planktonic production and respiration: a test of reservoir zonation theory. North American Lake Management Society, Hartford, Conn. Seagraves, SE, M Mullins, M Alexander, and RD Doyle. 2009. A Texas treasure: native aquatic plant restoration in a spring-fed river. Freshwater Mollusk Conservation Society, Baltimore, MD. Doyle, EI, RD Doyle and G Gonzales. 2009. Health and Drinking Water in Rural Southeast Brazil-A CBPR Study. American Association of Health Education. Tampa, Florida. Doyle, E. I., Doyle, R.D., Deike, E., and Hartman, J. 2008. Using the Global School-Based Student Health Survey among Brazilian Adolescents. SOPHE, October 2008. Sheppard (Davis) M., Doyle, E.I., Lutz, R., Doyle, R.D., Usdan, S.L. 2008. Mobilizing the community through community-based participatory research in Brazilian Churches. The American Public Health Association, 136th Annual Meeting & Exposition (October 25-29, 2008), San Diego, CA Forbes, M.G, R.D. Doyle, B. Hunter, J. Yelderman, B. Brooks, and S. Usenko. 2008. Evaluating water quality and water storage functions of coastal prairie freshwater wetlands in the HoustonGalveston Bay area. Soc. Wetland Scientist May, Washington DC, May 26-30. Nitrogen dynamics along a nitrate concentration gradient in a created freshwater wetland. 2008. R.D. Doyle, J. Thad Scott, Mark J. McCarthy, Wayne S. Gardner. Soc. Wetland Scientist May, Washington DC, May 26-30. Doyle, R.D., M. Forbes, T. Scott, T. Conry. 2008. Water Quality Benefits of the Lake Waco Wetlands. Invited presentation TCEQ/TOWTRC Annual Meeting, March 4-5, Waco. Davis, M., Doyle, E. I., Lutz, R., & Doyle, R.D. 2008. A Delphi Study of Key Informants: Can Brazilian Churches Promote Community Health? Annual Convention of the American Alliance for Health, Physical Education, Recreation and Dance, Fort Worth, TX, April 8-12, 2008. Davis, M., Doyle, E.I.,Lutz, R., & Doyle, R.D. 2007. A descriptive look at the health status of Brazilian church members through the use of community based participatory research. Annual Convention of the Alabama Alliance for Health, Physical Education, Recreation and Dance, Birmingham, AL, November 13, 2007. Doyle, R.D. M. Forbes, J. Belden, B.W. Brooks. 2007. Quantifying the Water Quality Functions of Wetlands. Invited presentation, Galveston Bay Estuary Program’s Eighth Biennial State of the Bay Symposium. Galveston, TX. Doyle, R.D., M.L. Mullins, Schell N.Y., 2007. Marsh madness: a science education adventure focused on wetland environments. ASLO, February 2007, Santa Fe, NM. Doyle CV- 2012 Page 9 of 16 Huang, H., R.D. Doyle, J.T. Scott, 2007. Spatial and temporal patterns of planktonic and community metabolism in Texas reservoirs: a test of the reservoir zonation model. ASLO, February 2007, Santa Fe, NM. Scott, J.T. Doyle, R.D., Prochnow, S.J., White, J. D., 2007. Watershed and pelagic predictors of Cyanobacterial n2 fixation in a eutrophic Reservoir. ASLO, February 2007, Santa Fe, NM. Conry, S., M.L. Mullins, R.D. Doyle. 2007. The distribution and expansion of Arundo donax (giant reed), an invasive riparian plant species, along the Lake Brazos corridor. Texas Academy of Science, March 2007, Waco, TX. Mullins, M.L. R.D. Doyle. 2007. Restoring aquatic plant communities: the San Marcos River experience. Texas Academy of Science, March 2007, Waco, TX. Doyle, R.D., M.L. Mullins, 2007. Marsh madness: a science education adventure focused on wetland environments. Texas Academy of Science, March 2007, Waco, TX. Scott, T., R.D. Doyle, J. White, S. Prochnow. 2006. Identifying watershed and pelagic thresholds favoring planktonic N2 fixation in a eutrophic reservoir: A combined mechanistic-empirical modeling approach. North American Lake Management Society, November 8-11. Indianapolis, IN. Mullins, M., and R.D. Doyle, 2006. Aquatic macrophyte restoration in the San Marcos river following removal of an invasive species. TX Society for Ecological Restoration, August 18-20, Hunt, TX. Scott, T. and R.D. Doyle. 2006. Identifying watershed and pelagic thresholds favoring planktonic n2 fixation in a eutrophic reservoir: a combined mechanistic-empirical modeling approach. TRRMS, May 18-19, Austin, TX. Scott, T. and R.D. Doyle. 2006. Coupled photosynthesis and bacterial biomass production in a nutrientlimited wetland periphyton mat. Society of Wetland Scientists, 27th International Meeting, Cairns, Australia. Huang, H., and R.D. Doyle, 2006. Patterns of planktonic and community metabolism along the riverinelacustrine gradient in texas reservoirs. TRRMS, May 18-19, Austin, TX. Conry, S., M. Mullins, and R.D. Doyle, 2006. Expansion of the invasive riparian plant, the giant reed (Arundo donax), along the lake brazos corridor. TRRMS, May 18-19, Austin, TX. Doyle, R.D., M Mullins, P Power, V Cantu, 2005. A Central Texas restoration project-- removal of an exotic aquatic macrophyte in a spring-fed system. International Spring Symposium, Mexico. Doyle, R.D. M. Mullins. 2005. Restoration of the lower San Marcos River: Removal of the exotic plant Cryptocoryne beckettii and re-establishment of natives. (SWS Regional Mtg, San Marcos, TX.) Doyle, R.D., S. Conry, and J.T. Scott. 2005. Lake Waco Wetland: habitat, research and water quality. (SWS Regional Mtg, San Marcos, TX.) J. Thad Scott and Robert D. Doyle, 2005. Enzymatic Controls On Metaphyton Primary Production And Cnp Stoichiometry In A Created Wetland. TRRMS Annual Meeting, Baylor University. Scott, J.T. and R. Doyle. 2005. Nitrogen Fixation and natural abundance of nitrogen isotopes in wetland periphyton communities. (SWS Regional Mtg, San Marcos, TX. Winner student competition w/ airfare to international SWS in Australia!). Doyle, R.D. J.T. Scott, and T. Conry, 2005. Can the Wetlands Save the Lake? The Role of a Constructed Wetland in Protecting the Water Quality of Lake Waco. 9th International Symposium on Biogeochemistry of Wetlands, March 20-23, 2005, Baton Rouge, Louisiana Doyle, R.D., T. Conry, J.T. Scott. 2005. Baylor university Center for Reservoir and Aquatic Systems Research (CRASR): promoting interdisciplinary aquatic research and education. ASLO winter meeting, Salt Lake City. Doyle, R. D., J.T. Scott, and T. Conry. 2005. Planktonic nitrogen fixation in a texas reservoir: hot spots and hot moments. ASLO winter meeting, Salt Lake City. Scott, J.T. R. Doyle, T. Conry. 2005. Periphyton nutrient limitation and nitrogen fixation along a differential wetland nutrient depletion gradient. ASLO winter meeting, Salt Lake City. Doyle CV- 2012 Page 10 of 16 Scott, T., R.D. Doyle, T. Conry. 2004. Periphyton nutrient limitation and nitrogen fixation potential along a differential n and p depletion gradient in a freshwater marsh. (Society of Wetland Scientists, Seattle Washington) Scott, T., R.D. Doyle, T. Conry. 2004. Periphyton nutrient limitation and nitrogen fixation along a differential wetland nutrient depletion gradient. (Am. Soc. Limnology and Oceanography, Savannah, GA.) R.D. Doyle, Scott, T., T. Conry. 2004. Planktonic nitrogen fixation in a texas reservoir: hot spots and hot moments. (Am. Soc. Limnology and Oceanography, Savannah, GA.) Scott, T., R.D. Doyle, and T. Conry. 2004. Spatial Trends In Periphyton Nutrient Status: Examination Along A Differential Nutrient Depletion Gradient In A Freshwater Marsh. (TX Academy of Science Meeting, Kerrville, TX) Swindle, R. and R.D. Doyle, Baylor University, 2004. Macrophyte Community Change In The San Marcos And Comal River. (TX Academy of Science Meeting, Kerrville, TX) Doyle, R.D. and T. Conry. 2004. Planktonic Nitrogen Fixation In Lake Waco: Hot Spots And Hot Moments. (TX Academy of Science Meeting, Kerrville, TX) Scott, T., R.D. Doyle, T. Conry. 2003. Periphyton Response to Total Nutrient Depletion and Shifting N:P Ratio in a Constructed Freshwater Wetland. (International conference of the Society of Environmental Toxicology and Chemistry, Austin, TX). Scott, T., R.D. Doyle, T. Conry. 2003. The impact of shifting N:P ratio on periphyton function along a nutrient depletion gradient in a constructed freshwater marsh. Annual meeting of the South Central Chapter of the Society of Wetland Scientists, Lake Texoma, OK. Doyle, R.D. and Power, P. 2003. Expansion of Cryptocoryne beckettii on the San Marcos River of Texas threatens Zizania texana, a federally listed aquatic plant species. Texas Watch Flood Symposium, San Marcos, TX (invited paper). Doyle, R.D. 2003. The bad guys win again: impacts of a major flood disturbance on the aquatic plant community of a spring-fed river. 7th International Conference on the Ecology and Management of Alien Plant Invasions, Ft Lauderdale, November. Doyle, R.D. & P. Power. 2003. Expansion of Cryptocoryne beckettii on the San Marcos River of Texas threatens Zizania texana, a federally listed aquatic plant species. 7th International Conference on the Ecology and Management of Alien Plant Invasions, Ft Lauderdale, November. Doyle, R.D. & P. Power, 2003. Texas Wildrice (Zizania texana) of the San Marcos River, TX: Can this species be saved? International meeting of the Society of Wetland Scientists, New Orleans, LA. Doyle, R.D. T. Scott, T. Conry, R. Howard, 2003. Can the wetland save the Lake? International meeting of the Society of Wetland Scientists, New Orleans, LA. Doyle, R.D. 2003. Ecological restoration of Texas aquatic plant communities: establishing desirable native species. TX Academy of Sciences. Doyle, R.D. 2002. Control of Cryptocoryne beckettii on the San Marcos River, options and progress. TX Aquatic Plant Management Society, Conroe, TX. Doyle, R.D. 2002. Cryptocoryne beckettii: A Apretty@ aquarium plant threatens endangered aquatic plant species in the San Marcos River, TX. Aquatic Plant Management Society, Keystone, Colorado. Doyle. R.D., M. Grodowitz, R.M. Smart, and C. Owens. 2001. Impacts of Hydrellia pakistanae on the growth and photosynthetic potential of Hydrilla verticillata. TX Aquatic Plant Management Society, Victoria, TX. M. Grodowitz and R. Doyle. 2001. Introduction to Biocontrol. TX Aquatic Plant Management Society, Victoria, TX. Doyle. R.D. and P. Power. 2001. Ecology and management of Zizania texana (Texas Wild Rice), an endangered aquatic macrophyte of the San Marcos River, Texas. Ecological Society of America. Doyle. R.D., M. Grodowitz, R.M. Smart, and C. Owens. 2001. Impacts of Hydrellia pakistanae on the growth and photosynthetic potential of Hydrilla verticillata. Aquatic Plant Management Society, Minneapolis MN. Doyle CV- 2012 Page 11 of 16 Doyle, R.D. and R.M.Smart. 2001. Hydrilla verticillata: distribution, impacts, and ecological considerations for the control of this exotic aquatic macrophyte. 104th Annual meeting, Texas Academy of Science, San Marcos, TX (invited presentation) Doyle, R.D. and P. Power. 2001.Ecology and management of Texas Wild Rice and the aquatic plant community of the San Marcos River, Texas. 104th Annual meeting, Texas Academy of Science, San Marcos, TX (invited presentation) Doyle, R.D. and M. Smart. 2000. Flood disturbance increase abundance of non-native aquatic macrophytes in Texas rivers. 20th International Symposium of the North American Lake Management Society, (Miami, FL). Doyle, R.D. 2000 Potential control of Hydrilla verticillata by use of an insect biocontrol agent in combination with a competitive native species. TX Aquatic Plant Management Society (Denton, TX). Doyle, R.D., M. Smart, M. Grodowitz. 2000. Potential control of Hydrilla verticillata by use of a classical biocontrol agent in combination with a competitive native species. III International Weed Science Congress, invited presentation, (Iguacu, Brazil). Doyle, R.D. and M. Francis. 2000. Impact of the flood of 1998 on the aquatic plant communities of the Comal and San Marcos rivers, TX. 103rd Meeting Texas Academy of Science (Kingsville, TX). Doyle, R.D., 1999. Protecting the Native Aquatic Plant Communities of the Comal and San Marcos River, TX. 39th International Meeting of the Aquatic Plant Management Society (Asheville, NC.) Grodowitz, M., R.D. Doyle, R. M. Smart. 1999. Effects of biocontrol agents on the competitive interactions between nuisance exotics and desirable native aquatic plants. 39th International Meeting of the Aquatic Plant Management Society (Asheville, NC.). Doyle, R.D., P. Power, and K. Kennedy. 1999. Assessment of Reintroduction Potential of Texas Wildrice (Zizania texana) into the San Marcos River. Wild Rice: Research and Management Conference, (Duluth, MN.) Francis, M. and R.D. Doyle. 1998. Intraspecific competition between Hygrophila polysperma and Ludwigia repens in the Comal River, New Braunfels, TX (Denton, TX). Pennington, T., A.W. Groeger, P.Power, and R.D. Doyle. 1998. Identifying nutrient content and composition to further characterize the habitat associated with the endangered Texas Wildrice (Zizania texana Hitch). Texas River and Reservoir Management Society (Denton, TX). Doyle, R.D. P. Power, P. Connor. 1998. Assisted Recovery of Texas Wildrice (Zizania texana) in the San Marcos River. Society of Ecological Restoration (Austin, TX). Smart, R.M.,. G. Dick, and R.D. Doyle. 1998. Ecological Effects of Native Aquatic Plants. 38th Meeting of the Aquatic Plant Management Society (Memphis, TN) Grodowitz, R.M., R.D. Doyle, M. Smart. 1998. Effects of biocontrol agents on the competitive interactions between nuisance exotics and desirable native aquatic plants. 38th Meeting of the Aquatic Plant Management Society ( Memphis, TN). Forbes, M., R. Doyle, T. Conry, H. Chen. 1998. Wetland creation in Sugarland, TX: building a hydrophytic community. Society of Wetland Scientist (Memphis, TN) Doyle, R.D. 1998. Effects of inorganic turbidity on the growth and reproductive potential of native macrophytes. American Society of Limnology and Oceanography (St. Louis, MO). Doyle, R.D. and R.M. Smart. 1997. Role of aquatic macrophytes in reservoir ecosystems.17th International Symposium of the North American Lake Management Society, (Houston, TX) Doyle, R.D. 1997. Effects of inorganic turbidity on the growth and reproductive potential of native macrophytes. Aquatic Plant Management Society Meeting (Fort Meyers, Florida.) Doyle, R.D. and R.M. Smart. 1996. Establishing Native Vegetation in Southern Reservoirs: Why and How. Aquatic Plant Management Society Meeting, (Burlington, VT) Doyle, R.D., R. M. Smart, G.O. Dick, and M. Webb. 1996. Ecological restoration of Lake Conroe, TX: Establishment of native submersed aquatic macrophytes. Texas Academy of Sciences. Galveston. Doyle CV- 2012 Page 12 of 16 Doyle, R.D., and R.M. Smart. 1995. Restoration and rehabilitation of Texas reservoirs: the role of native aquatic macrophytes. North American Lake Management Society 15th International Symposium. (Toronto, Canada) Smart and R.D. Doyle. 1995. Propagation and planting of desirable aquatic plant species. Texas Aquatic Plant Management Society. (Galveston, TX) Doyle, R.D., R.M. Smart, and C. Guest. 1995. Establishing native macrophytes for fish habitat in North Texas Reservoirs. Texas Chapter American Fisheries Society. (College Station, TX) Doyle, R.D. 1995. Hydrilla infestations in Texas Reservoirs: The problem of controlling dormant tubers. Texas Academy of Science. (Waco, TX) Doyle, R.D. 1994. Establishment of native submersed aquatic plants in Texas reservoirs for habitat enhancement. Texas River and Reservoir Management Society Meeting. (Waco, TX) Doyle, R.D. and R.M. Smart. 1994. Field tests of the use of native aquatic plants to control Eurasian watermilfoil in a southeastern reservoir. North American Lake Management Society 14th Annual International Symposium, (Orlando, FL) Doyle, R.D. and R.M. Smart. 1994. Native Emergent Macrophytes may ameliorate the negative impacts of Lyngbya. North American Lake Management Society 14th Annual International Symposium, (Orlando, FL) Smart, R.M. and R.D. Doyle. 1994. Establishment of native submersed aquatic plants and their potential for resisting invasion by exotic, weedy species. North American Lake Management Society 14th Annual International Symposium, (Orlando, FL) Doyle, R.D. and R.M. Smart. 1994. Use of Native Aquatic Plants for Control of Nuisance Species in Guntersville Reservoir, Alabama. The Aquatic Plant Management Society, Thirty-Fourth Annual Meeting, (San Antonio, TX) Power, P., and R.D. Doyle. 1994. Carbon sources for submerged leaves of the endangered Zizania texana (Texas Wildrice). The Aquatic Plant Management Society, Thirty-Fourth Annual Meeting, (San Antonio, TX) Doyle, R.D., T. Myers, and D. Adrian. 1993. Atrazine Retention and Disappearance in Experimental Wetland Mesocosms. 2nd Biennial Symposium on Biogeochemistry of Wetlands. (LSU) Doyle, R.D. and R.M. Smart. 1993. Competitive ability of selected aquatic macrophytes in Lyngbya infested areas of Lake Guntersville, AL. North American Lake Management Society Conference. (Chattanooga, TN) Doyle, R.D. 1993. Creating wetlands at Lake Ray Roberts: objectives, design, vegetation, and cost. Texas Lake and Reservoir Management Society Annual Conference. (Dallas, TX) Doyle, R.D., and T.R. Fisher. 1992. Biomass and nitrogen uptake of macrophyte/periphyton complex in an Amazon floodplain lake. INTECOL's IV International Wetlands Conference. (Columbus, OH) Doyle, R.D., and M. Smart. 1992. Establishment success and competitive ability of selected aquatic macrophytes in Lyngbya-dominated littoral zones of Guntersville, Alabama. Mid-South Aquatic Plant Management Society Meeting. (Guntersville, AL) Doyle, R.D., K. Dickson, D. Sturges, and M. Smart. 1992. Created wetlands on Lake Ray Roberts, TX: Design, pre-impoundment status, and research opportunities. Soc. of Wetland Scientists, (New Orleans, LA) Doyle, R.D. 1992. Nitrogen fixation by periphyton on the Amazon floodplain. ASLO, (Santa Fe, NM) Fisher, T.R., R.D. Doyle, M. Moline, and D.L. Engle. 1990. Ecology of periphyton on the Amazon floodplain. Symp. Floodplain Rivers. (LSU. Baton Rouge, LA) Doyle, R.D., and T.R. Fisher. 1988. Photosynthesis and respiration of periphyton on the Amazon floodplain. ASLO/AGU Ocean Science Meeting (Dec). Fisher, T.R., L. Alves, R.D. Doyle, M. Moline, E. Peele, L. Smith-Morrill. 1988. Periphyton on the Amazon River floodplain. ASLO/AGU. Fisher, T.R., E.R. Peele, and R.D. Doyle. 1988. Phosphorus Dynamics in the Chesapeake Bay. ASLO/AGU Doyle CV- 2012 Page 13 of 16 Fisher, T.R., L. Smith-Morrill, R.D. Doyle, and E.R. Peele. 1988. Productivity and nutrient cycling on the Amazon's floodplain. Chapman Conference on Amazon Dispersal Systems. Doyle, R.D., T.R. Fisher, and E.R. Peele. 1987. Biomass and productivity of periphyton on the Amazon's floodplain. ASLO. Fisher, T.R., R.D. Doyle, and E.R. Peele. 1987. Uptake and regeneration of phosphate on the Amazon's floodplain. ASLO. Fisher, T.R., R.D. Doyle, and E.R. Peele. 1987. Size-fractionated uptake and regeneration of ammonium and phosphate in a tropical lake. SIL Congress. New Zealand. Fisher, T.R. and R.D. Doyle, 1987. Nutrient cycling in Chesapeake Bay. MD Sea Grant conference on PART III. PROFESSIONAL MEMBERSHIP AND SERVICE oxygen in the Chesapeake Bay and related processes. Fisher, T.R. and R.D. Doyle. 1986. N and P cycling in an Amazon River floodplain lake. Freshwater Wetlands and Wildlife Symp. Univ. of Georgia. Doyle, R.D. and L. Davalos. 1985. Phytoplankton production in a large tropical lake (Lago Calado, Mexico) II. factors controlling biomass and production. 48th ASLO meeting. MEMBERSHIP IN PROFESSIONAL ORGANIZATIONS: American Society of Limnology and Oceanography, Society of Wetlands Scientist, Aquatic Plant Management Society, North American Lake Management Society, Society for Ecological Restoration Texas River and Reservoir Management Society, Texas Aquatic Plant Management Society, Texas Academy of Sciences, Fellow EDITORIAL AND REVIEW ACTIVITIES: Associate Editor: (2000-2002) Associate Editor of Wetlands (peer reviewed journal of the Society of Wetlands Scientists) Manuscript Reviewer for Journals: Aquatic Botany. (International peer-review journal, highest rated journal in aquatic botany field). Lake and Reservoir Management. (Peer-reviewed journal of the North American Lake Management Society) Journal of Aquatic Plant Management, (Peer-reviewed journal of the Aquatic Plant Management Society). Wetlands (Peer-reviewed journal of the Society Wetland Scientists). Hydrobiologia (International peer-review journal). Sida: Contributions to Botany, (National peer-review journal). Journal of Environmental Quality (International peer-reviewed journal) Estuaries (International peer-reviewed journal) Science of the Total Environment (peer-reviewed journal) Grant Reviews: National Fish and Wildlife Foundation UNT/Baylor Faculty Research Grant Reviewer. International Technical Referee, New Zealand Foundation for Research, Science and Technology. New Zealand Aquatic Plant Management Program Review (1998-2000) Technical Review Panel, Ohio Sea Grant Program. Doyle CV- 2012 Page 14 of 16 Technical Review Panel, Western Region=s Water Resources Research Competitive Grants Program. Technical Review Panel, EPA Region 6. Other Review Activities: USDA, Manuscript peer reviewer. US Army Engineers Waterways Experiment Station, Technical Reviewer CURRENT MEMBER OF STATE AND FEDERAL TECHNICAL WORKING GROUPS EPA Regional Technical Assistance Group (RTAG) for Nutrient Criteria (the RTAG provides guidance to the States in developing numeric criteria for N and P as mandated under the Clean Water Action Plan) USFWS San Marcos River Recovery Team (Advisory Team, a group of recognized experts in science, policy, and law that provide direction for efforts to restore the San Marcos River). USFWS Texas Wildrice Recovery Team (group of recognized experts in the ecology of the endangered Texas Wildrice Zizania texana, working to restore the endangered aquatic plant to the San Marcos River, TX. ). Cryptocoryne beckettii Eradication Working group- group of scientists that provide direction for efforts to eradicate this species from the San Marcos River. OFFICES AND COMMITTEE ASSIGNMENTS IN PROFESSIONAL ORGANIZATIONS: National/International Organizations 1997-2001 Aquatic Plant Management Society, Liaison to the North Am. Lake Man. Society 1997 North American Lake Management Society, (Chair Conference Planning Committee for 17th International Symposium). 1997 North American Lake Management Society. 17th International Symposium, Houston, TX. (Program Chair) State/Regional Organizations 2007 Host organizing committee, TX Academy of Sciences. 2000-2003 TX Aquatic Plant Management Society, Board of Directors 2000 Chair program committee of the TX Aquatic Plant Management Society 1998- 2001 Texas River and Reservoir Management Society. Past-Pres. and Chair of Nominating Committee 1998- 2000 Texas Academy of Science, Board of Directors 1998 Texas River and Reservoir Management Society (Member of Coordinating Committee that organized the annual meeting of the society at UNT) 1997 Texas River and Reservoir Management Society, President and member “NALMS 97" Conference Coordinating Committee. 1997 EPA Region 6 Second Watershed Conference, Denton (Coordinating Committee) 1994-1997 Texas River and Reservoir Management Society, Board of Directors. 1996 North American Lake Management Society Region 6, Fort Worth, TX (Program Chair of First Regional Conference) EXPERT PARTICIPATION: 2005 National Research Panel to Evaluate Needs of Invasive Aquatic Plant Management in North America, invited expert (La Jolla, CA, 2005). 2004 Restoration and Management of Riparian Corridors, TAES Blackland Research Center, invited instructor on stream restoration. Doyle CV- 2012 Page 15 of 16 2004 San Marcos Recovery Team 2003 Golden Algae Workshop, TPWD 10/24-25, Fort Worth, TX (invited participant) 1998 Texas Parks and Wildlife Department Aquatic Vegetation Committee, ABass n= Grass@, forum for discussion of effects of vegetation on reservoir fisheries (invited aquatic vegetation expert). 1998 US Army COE, AUpper Mississippi River Project: Plant Modeling Workshop@, Oak Ridge, TN, (invited expert on the effects of inorganic turbidity on growth of submersed macrophytes). 1997 US Army COE, AModeling Integration and Simulation Team Meeting@, New Orleans, LA. (invited to provide information on the affects of clay turbidity on the growth of aquatic plants). 1997 Maryland Department of Natural Resources, AChesapeake Bay Submerged Aquatic Vegetation Re-seeding and Transplanting Workshop@, Belvue, MD., (invited national aquatic plant expert). 1996 Southwest Florida Water Management District, ARainbow River - Lyngbya Workshop@, Gainesville, FL., (invited expert on the use of macrophytes to control mat-forming algae). 1996 US Army COE, AUpper Mississippi River Project: Plant Modeling Workshop@, Oak Ridge, TN, (invited expert on the effects of inorganic turbidity on growth of submersed macrophytes). 1996 U.S. Fish and Wildlife Service, ASan Marcos and Comal Spring Ecosystem Viability Assessment and Management Planning: Texas Wild-rice Group@, Austin, TX. (invited aquatic plant expert). 1995 US EPA National Conference for Enhancing the State=s Lake Management Programs, Chicago, (representative of the Texas River and Reservoir Management Society, Texas= NALMS affiliate) 1994 4th Annual Review Meeting of the Guntersville Joint Agency Project (Tennessee Valley Authority - Corps of Engineers), Vicksburg, MS, (participant) 1994 US Army COE Aquatic Plant Control Research Program, Vicksburg, MS (research presentation) 1993 US Army COE Aquatic Plant Control Research Program, Baltimore MD (research presentation) 1993 3rd Annual Review Meeting of the Guntersville Joint Agency Project (Tennessee Valley Authority - Corps of Engineers), Guntersville, AL, (technical paper presentation and participant) 1992 US Army Corps of Engineer Aquatic Plant Control Research Program, Bellevue, WA. (technical paper presentation and participant) 1992 2nd Annual Review Meeting of the Guntersville Joint Agency Project (Tennessee Valley Authority - Corps of Engineers), Guntersville, Alabama, (technical paper presentation) PART IV. TEACHING AND STUDENT DEVELOPMENT COURSES TAUGHT AT BAYLOR Although I currently have a high administrative load (Chair Department of Biology (50%) and Director Center for Reservoir and Aquatic Systems Research (12.5%)) I continue to teach at least one course most semester. I very much enjoy teaching and my student evaluations always exceed comparison norms. Co-director, Baylor in Brazil Summer Abroad (2006-2011) Dr. Eva Doyle and I direct a summer abroad educational program. Courses taught at Baylor on a regular rotation: Wetland Ecology and Management (Bio 5304), graduate course Restoration Ecology (Bio 4381), Senior level course for majors Principles of Biology II (Bio 1306), Freshman level course for majors Other courses taught Stream Ecology, Senior level majors’ course Doyle CV- 2012 Page 16 of 16 Ecology, Junior level majors’ course COURSES TAUGHT AT UNT Aquatic Botany (BIOL 5040, 4 credit hour graduate course in aquatic plant ecology) Wetland Ecology (UNT= BIOL 5040/4980, 4 credit hour course, senior and graduate level) Environmental Health. (UNT= BIOL 5200, 3 hour graduate course). Principles of Biology II (UNT BIOL 1720/1722, Botany/Ecology Section- introductory majors biology course) THESIS/DISSERTATION ADVISEMENT: Chair or Co-Chair, Advisory Committee Melissa Mullins (PhD, ongoing BU) Rachel Bormann (M.S. ongoing BU) Thad Scott (Ph.D., 2006 BU) Hui Huang (M.S., 2006 BU) Robin Bare (M.A. 2005 BU) Shannon Mandolin (M.S. Env Studies, 2004 BU) Maggie Forbes (Ph.D. 2002 UNT co-advisor) Tessy Baugher (MS, 2001 UNT) JoEtta Smith (MS, 2001UNT) Joe Snow (MS, 2000 UNT) Matt Francis (MS, 1999 UNT) PUBLIC EDUCATION: 2003-10 Master Naturalist training- HOT chapter. 2002 Baylor University Continuing Education- AAquatic Ecosystems 1998-99 Instructor for AMaster Naturalist Program of North Texas offered by Texas Agriculture Extension Service. Six hour course on Aquatic Ecology. 1998 Instructor for Master Gardner- Ecologist Training offered by Texas Agriculture Extension Service, Denton, TX. 1996 Instructor for short course offered at the American Fisheries Society Meeting, Mobile, AL. Feb 24-25. AMethods for Establishing Native Aquatic Plants for Fisheries Benefits OTHER SERVICE ACTIVITIES: 2001-present City of Waco Lake Waco Wetland Advisory Board 1998-2001 Lewisville Lake Environmental Learning Area Board of Directors, Board Member 1997-2001 Heard Museum Environmental Education Board (McKinney, TX) 1992-1996 Lewisville Lake Environmental Learning Area Board of Directors & President.