Roots Agronomy for Women in Farm Business
Transcription
Roots Agronomy for Women in Farm Business
A review of Research Projects supported by the GRDC in 2013-14 Theme 6 Building Skills and Capacity Table of Contents Introduction.........................................................................................................................11 AAA00007: ABCA 2012-13 and 2013-14..............................................................................15 ARL00007: Australian Rural Leadership Foundation Limited ...................................................16 ARL00008: Australian Rural Leadership Program ..................................................................17 DAN00185: Post Doctoral Fellow - Soil nutritional chemistry in southern grains farming systems ..18 DAS00142: Traineeships in Applied Grains Research ............................................................19 DER00018: GRDC Network Survey 2013.............................................................................20 GCS10433: Improved Adaptation to Drought.........................................................................21 GCS10750: Conference Sponsorship - Grass-Roots Agronomy for Women in Farm Business......22 GCS10751: Conference Sponsorship - Extending life cycle assessment (LCA) to include soil ......23 GCS10752: Conference Sponsorship - BCG Grains Research Expo ........................................24 GCS10754: Conference Sponsorship - International Association of Plant Biotechnology 2014......25 GCS10755: Conference Sponsorship - Australasian Plant Breeding Conference 2014 ................26 GCS10757: Conference Sponsorship - Farmsafe - Working for the Future ................................27 GCS10759: Conference Sponsorship - Sydney Royal Easter Show - Grains education displays ...28 GCS10760: Conference Sponsorship - SAGA 2014 Industry Day - Life beyond Glyphosate.........29 GCS10761: Conference Sponsorship - 2014 SANTFA Conference ..........................................30 GCS10762: Conference Sponsorship - SEPWA Harvest Review and Season Preview, 2014 .......31 GCS10764: Conference Sponsorship - Tasmanian Farmers & Graziers Association 2014 Major Conference......................................................................................................................32 GCS10765: Conference Sponsorship - 17th Australian Nitrogen Fixation Conference .................34 GCS10766: Conference Sponsorship - 2014 International Plant Cell Wall Meeting .....................35 GCS10767: Conference Sponsorship - 8th Australasian Soilborne Diseases Symposium (8ASDS) ......................................................................................................................................36 6. Building Skills & Capacity GCS10768: Conference Sponsorship - 11th Australasian Plant Virology Workshop ....................37 2 GCS10769: Conference Sponsorship - Digital Rural Futures Conference..................................38 GCS10771: Conference Sponsorship - Victorian Farmers Federation Annual Grains Conference .39 GCS10774: Conference Sponsorship - AGSA - AGSA2014 ....................................................40 GCS10778: Conference Sponsorship - UNFS - Annual Field Day ............................................41 GCS10789: Conference Sponsorship - NCMC -Boosting Productivity in North-West NSW ...........42 GCS10790: Conference Sponsorship - MSF Research, Development & Extension Tri-State Forum ......................................................................................................................................43 GCS10797: Conference Sponsorship – WA Farmers 2014 Annual Conference - Grains Section...44 GGA00003: Grain Gain--Leadership for Grains Industry Innovation ..........................................45 GRS10004: Grains Industry Research Scholarship - Alice Burgess (ANU) Identifying novel epigenetic components that regulate seed size in a model plant, Arabidopsis.............................46 GRS10026: Grains Industry Research Scholarship - Foyjunnessa (UA) Assesing management options for enhancing soil phosphorus availability using rotations .............................................47 GRS10027: Grains Industry Research Scholarship - Heidi Waddell (UWA) Phosphorus-use efficiency of Austrodanthonia..............................................................................................49 GRS10028: Grains Industry Research Scholarship - Hollie Webster (UMU) Genetic factors and genes underpinning drought response in wheat.....................................................................50 GRS10029: Grains Industry Research Scholarship - Karl Andersson (UNE) Manipulation of P sorption in agricultural soils ................................................................................................52 GRS10031: Grains Industry Research Scholarship - Laura Blake (UA) Use of novel wheat (waxy durum) in baking applications .............................................................................................53 3 GRS10034: Grains Industry Research Scholarship - Luke Holtham (UA) Identification of the Controller of Nitrate Transport in Maize ................................................................................54 GRS10036: Grains Industry Research Scholarship - Maree Horne (USQ) Inter-relationships between Bipolaris sorokiniana isolates involved in spot blotch, common root rot and black point in winter cereals...................................................................................................................56 GRS10037: Grains Industry Research Scholarship - Michael Ashworth (UWA) Evolved Glyphosate resistance in wild radish (Raphanus raphanistrum L) populations with the use of Glyphosate resistant genetically modified canola....................................................................................57 GRS10038: Grains Industry Research Scholarship - Mirja Guldner (US) Enhancing plant nutrition with rhizosphere microorganisms ........................................................................................59 GRS10039: Grains Industry Research Scholarship - Monica Kehoe (UWA) Unraveling the cause of black pod disease of narrow-leafed lupin and developing a control solution ...............................60 GRS10040: Grains Industry Research Scholarship - My-my Huynh (UF) Examining the role of sucrose transporter HvSUT1 in increasing yield and iron/zinc content in Rice ............................61 GRS10041: Grains Industry Research Scholarship - Peter Gous (UQ) Understanding abiotic stress impacts on cereal starch structure and value-added quality through genetic and environmental screening ........................................................................................................................62 GRS10042: Grains Industry Research Scholarship - Peter McEntee (CUR) The integration and validation of precision management tools for mixed farming systems. .......................................63 GRS10044: Grains Industry Research Scholarship - Rhiannon Schilling (UA) Evaluating the salt tolerance of transgenic wheat and barley..............................................................................64 GRS10045: Grains Industry Research Scholarship - Ryan Kearns (UQ) Genetic variability and physiological mechanisms controlling time to flowering in wheat under high temperatures ...........65 GRS10061: Grains Industry Research Scholarship - Robert Syme (CUR) Comparative genomics of necrotrophic fungal pathogens ............................................................................................66 GRS10063: Grains Industry Research Scholarship - Oliver Buddrick (RMIT) Bread making procedure, product digestibility and impact on sensibility to gluten proteins................................67 GRS10248: Grains Industry Research Scholarship - Amanda Huen (US) Regulation and longdistance movement of nutrient starvation-responsive plant microRNAs .....................................68 GRS10249: Grains Industry Research Scholarship - Andrew Kettle (UQ) Functional pathogen genomics and characterisation of the infection mechanisms of the wheat and barley crown rot pathogen, Fusarium pseudograminearum.............................................................................69 GRS10258: Grains Industry Research Scholarship - Ella Brear (US) Charcterising potential symbiosome membrane proteins essential to the legume-rhizobium symbiosis ..........................70 GRS10259: Grains Industry Research Scholarship - George Dimitroff (UA) (1,3;1,4)-?-D-glucan biosynthesis in the Poaceae: Exploring transcriptional regulation, associated expression and specific activities of biosynthetic enzymes.............................................................................71 GRS10263: Grains Industry Research Scholarship - Jessey George (UA) Effect of small amounts of ammonium on the total nitrogen nutrition and on overall nutrition of maize .................................72 GRS10268: Grains Industry Research Scholarship - Karthika Krishnasamy (UMU) Wheat potassium nutrition in saline and/or sodic soils and in drought-prone environments .....................73 GRS10275: Grains Industry Research Scholarship - Max Bergmann (UWA) The use of potassium to improve water usage, growth and yield of canola in European high-yielding and Australian lowyielding farming systems....................................................................................................74 GRS10290: Grains Industry Research Scholarship - Wenywati Tjong (RMIT) Forms, analysis and 6. Building Skills & Capacity GRS10035: Grains Industry Research Scholarship - Madeline Tucker (CUR) Minimising fungicide resistance........................................................................................................................55 stability of vitamin E and selenium in grains and grain products ...............................................75 GRS10329: Grains Industry Research Scholarship - Christina Delay (ANU) Investigating the role of root architecture regulators as mediators of environmental information in root development .........76 GRS10335: Grains Industry Research Scholarship - Courtney Peirce (UA) Foliar Fertilisation Of Wheat Plants - Phosphorus in Combination with Other Nutrients..............................................77 GRS10336: Grains Industry Research Scholarship - Tawanda Kapfuchira (US) Genetics of Biofortified Wheat .............................................................................................................78 GRS10404: Grains Industry Research Scholarship - Dustin Severtson (UWA) Detection and epidemiology of spring aphids in canola ...............................................................................79 GRS10405: Grains Industry Research Scholarship - Guoquan Liu (UQ) Manipulating seed storage proteins to enhance sorghum digestibility .............................................................................80 GRS10407: Grains Industry Research Scholarship - Juanita Lauer-Smith (UA) Characterisation of novel forms of beta-glucanase in malting barley ....................................................................81 GRS10408: Grains Industry Research Scholarship - Julia Andrys (UMU) Spatial and Temporal Distribution of Severe Weather Events and their Impact on Grain Crops....................................82 GRS10421: Grains Industry Research Scholarship - Daniel O'Connor (UQ) Development of functional molecular markers for key agronomic traits in the culitvated peanut, using nextgeneration sequencing technologies ....................................................................................83 GRS10432: Grains Industry Research Scholarship - Thomas Jason Major (ANU) Communicating controversial science to farmers ..........................................................................................84 GRS10437: Grains Industry Research Scholarship - Kasra Sabermenesh (UA) Responses of maize roots to nitrogen supply .....................................................................................................85 GRS10440: Grains Industry Research Scholarship - Kevin Adamson (Sunshine Coast) Investigation of metabolic regulatory genes and hormones in pest snails. ..................................86 GRS10460: Grains Industry Research Scholarship - Julia Man (UWA) Characterisation of Arabidopsis nitrilases and 14-3-3 interactions in the processes of photosynthetic loss, leaf senescence and plant stress ..............................................................................................87 GRS10471: Grains Industry Research Scholarship - Kylie Foster (USA) Whole-of-plant study of salinity tolerance: A mathematical modelling approach ...........................................................88 6. Building Skills & Capacity GRS10474: Grains Industry Research Scholarship - John Hayles (UA) Nanotechnology and chemical-free approach for the protection of stored grain ........................................................89 GRS10477: Grains Industry Research Scholarship - Volkan Arici (USQ) On-the-go proximal soil exchangeable ion sensing for precision management of sodicity ..............................................90 GRS10483: Grains Industry Research Scholarship - Aaron Preston (UCS) Determination of genetic markers for herbicide resistance in annual ryegrass using diversity array technology ..................91 GRS10490: Grains Industry Research Scholarship - Jana Phan (UA) Identification of arabionxylan biosynthetic genes in plants ...............................................................................................92 GRS10501: Grains Industry Research Scholarship - Kimberly Alexander (UQ) Identification of quantitative trait loci associated with heat tolerance in wheat for increased fertility and grain development/genetic variability in heat responses for grain development...................................93 GRS10505: Grains Industry Research Scholarship - Oliver Mead (ANU ) Investigating the role of gamma-aminobutryic acid in pathogenicity in fungal wheat diseases ........................................94 GRS10507: Grains Industry Research Scholarship - Reece Tollenaere (UQ) Identification of blackleg resistance genes in canola.....................................................................................95 4 GRS10512: Grains Industry Research Scholarship - Josh Chopin (USA) Mathematical and Computational Modelling for the Phenotypic Analysis of Cereal Plants ......................................96 GRS10532: Grains Industry Research Scholarship - Jonathan Powell (UQ) Identifying sources of resistance to necrotrophic plant pathogens using the model grass Brachypodium distachyon .......97 GRS10564: Grains Industry Research Scholarship - Alison Renwick (CUR) Effector gene prediction from fungal pathogen genome assemblies............................................................................98 GRS10566: Grains Industry Research Scholarship - Myrtille Lacoste (UWA) Understanding the strategies of outstanding performers in dryland farming enterprises ..........................................99 GRS10577: Grains Industry Research Scholarship - Kirt Hainzer Thesis research into the biological farming approach............................................................................................................ 100 5 GRS10578: Grains Industry Research Scholarship - Paul Kavanagh (US) Genetics of host: pathogen interactions in the Puccinia hordei: Hordeum vulgare pathosystem ........................... 101 GRS10660: GRS - Eisrat Jahan (US) Genetypic variation in mesophyll conductance of wheat (Triticum aestivum) and its effect on WUE .......................................................................... 103 GRS10661: GRS - Emma Thompson (UQ) Brewer Nutrient recovery from wastewater using electrodialysis ................................................................................................................ 104 GRS10664: GRS - Clare Flakelar (UCS) Canola quality - the influence of processing parameters on valuable minor components. ............................................................................................. 105 GRS10667: GRS - Fabian Inturrisi (UQ) Identification of candidate genes for blackleg resistance in Brassica Juncea............................................................................................................. 106 GRS10683: GRS - Diep Ganguly (ANU) What are the epigenetic mechanisms involved in regulating stress response in Arabidopsis thaliana and Brachypodium distachyon................................... 107 GRS10686: GRS - Jessica Mackay (UA) Biological farming systems: is there a role for mycorrhizas and organic amendments in the grain industry?................................................................... 108 GRS10687: GRS - John Rivers (ANU) RNA secondary structure and the regulation of metabolism .................................................................................................................................... 109 GRS10690: GRS - Kenton Porker (UA) Manipulating and understanding barley phenology to maximise yield potential................................................................................................... 110 GRS10691: GRS - Kylie Wenham (UQ) Investigations into the biology and management of Neocosmospora root rot of peanuts in Australia................................................................... 111 GRS10692: GRS - Nathan Craig (UWA) The influence of cereal monoculture and high crop residue levels on mineral nitrogen availability under long term no-tillage systems. ............................... 112 GRS10694: GRS - Sandra Kerbler (UWA) Investigating the molecular basis of thermal acclimation in plant mitochondria ....................................................................................................... 113 GRS10695: GRS - Sarah Lorberg (UQ) Novel sources of disease resistance in Brassica .......... 114 GRS10698: GRS - Thomas Simnadis (UW) Studies in Novel Grains for the Australian Food Supply .................................................................................................................................... 115 GRS10699: GRS - Vijira Wanniarachchi (UF) Alternative respiratory genes can improve tolerance to abiotic stresses in cereals ............................................ 116 GRS10780: GRS - Adam Taranto (ANU) Compontents of Immunity to Stagonospora nodorum in Wheat ........................................................................................................................... 117 GRS10781: GRS - Jayachandra Rongala (UA) Cereal Transport Proteins Involved In Boron Toxicity Tolerance: How Natural Variation And Sequence Diversification Relate To Structural And Functional Properties ...................................................................................................... 118 GRS187: Grains Industry Research Scholarship - Liza Parkinson (UMU) Investigating the method of action of plant growth promoting Rhizosphere bacteria enhancing nodulation in legumes ....... 119 GTA10022: Travel Award - Mr Pradeep Sornaraj (UF) The XV Congress on Molecular PlantMicrobe Interaction/Presenting data at the Conference ......................................................... 120 GTA10267: Travel Award - Julia Brueggemann (UA) Plant & Animal Genome XX .................... 121 GTA10348: Travel Award - Maarten Kooiker (CSP) International Plant Molecular Biology Congress .................................................................................................................................... 122 GTA10361: Travel Award – Alexandra Milner-Smyth (SAN) SANTFA Bus Trip to New South Wales .................................................................................................................................... 123 6. Building Skills & Capacity GRS10658: GRS - Ailisa Blum (UQ) Regulation of the production of the mycotoxin deoxynivalenol by Fusarium graminearum and its according virulence on wheat and barley............................. 102 GTA10368: Travel Award - Matthew Gilliham (UA) Conference Travel to US and visits researchers to strike up collaboration .................................................................................................. 124 GTA10385: Travel Award – Sarita Bennett (CUR) To present a paper at the 16th Australian Agronomy Conference..................................................................................................... 125 GTA10419: Travel Award - Bo Xu (UA) International Workshop on Plant Membrane Biology XVI 2013 ............................................................................................................................. 126 GTA10422: Travel Award - Kaitao Lai (UQ) PAG XXI Conference .......................................... 127 GTA10427: Travel Award - Roslyn Jettner (DAW) Understanding the China market and its requirement for Milling Oats - Industry Champion travel ........................................................ 128 GTA10428: Travel Award - Sandra Schmoeckel (UA) International Workshop on Plant Membrane Biology XVI 2013 ............................................................................................................ 129 GTA10430: Travel Award - Stephen Carr (Aglime) Gain a more detailed understanding of techniques used in encouraging farmers to apply adequate lime to remove soil acidity .............. 130 GTA10475: Travel Award - Michael Nash (UM) Attend conference and gain knowledge of current slug control research....................................................................................................... 132 GTA10498: Travel Award - Iain Searle (ANU) Keystone Conference- RNA Silencing ................ 133 GTA10499: Travel Award - Jiaen Qiu (ACPFG) International Workshop on Plant Membrane Biology XVI 2013 ....................................................................................................................... 134 GTA10511: Travel Award - Wenmian Huang (ACPFG) International Workshop on Plant Membrane Biology XVI 2013 ............................................................................................................ 135 GTA10579: Travel Award - Andrew Robson APRES Conference, project development with University of Florida, USDA and Birdsong Peanut ................................................................ 136 GTA10592: Travel Award - Huajian Liu 2013 Society for Engineering in Agricuture Conference .. 137 GTA10600: Travel Award - Keith Alcock Genetically Modified Crops Coexistence Conference, GMCC-13...................................................................................................................... 138 GTA10605: Travel Award - Dr Megan McDonald 10th International Congress of Plant Pathology 139 GTA10625: Travel Award – ICC-AGSA 2013 ...................................................................... 140 GTA10638: Travel Award - Scott Boden Attendence of International Wheat Genetics Symposium .................................................................................................................................... 142 6. Building Skills & Capacity GTA10643: Travel Award - Kim-Yen Phan Thien To participate in the 13th ASEAN Food Conference 2013 on Meeting Future Food Demands: Security and Sustainability ..................... 143 6 GTA10705: Travel Award - Ehsan Tavakkoli (UA) 20th World Congress of Soil Science (WCSS) 144 GTA10706: Travel Award - Elizabeth Kingston (WNRM) Resilience 2014................................ 145 GTA10707: Travel Award - Glen Fox (UQ) 23rd Institute of Brewing & Distilling Convention Asia Pacific Section (ht .................................................................................................... 146 GTA10709: Travel Award - Jason Terpolilli (UMU) The three objectives of this study tour are:1. Present my research paper at th ....................................................................................... 147 GTA10712: Travel Award - Julien Bonneau (UM) PAG ......................................................... 148 GTA10715: Travel Award - Manuel Zander (UQ) International Plant & Animal Genome XXII Conference.................................................................................................................... 149 GTA10717: The current status, potential applications and future directions of bacterial biocontrol research against Sclerotinia sclerotiorum of canola in Australia.............................................. 150 GTA10718: Travel Award - Muhammad Munir Iqbal (UWA) To present poster in Plant and Animal Genome (PAG) Conference, 2014 and study vi ................................................................... 151 GTA10721: Travel Award - Enli Wang, Zhongkui Luo, Hongtao Xing (CSO) 20th World Congress of Soil Science................................................................................................................... 153 GTA10786: Travel Award - Shi Ying Yang (UA) Attend the Plant Genomics Congress:Asia........ 154 ICN00017: Industry Pertnership Initiative ............................................................................ 155 IDA10355: Industry Development Award - Living Farming - Farming systems and the continued problem of herbicide resistance ........................................................................................ 156 IDA10441: Attendance & dissemination of info from the Global Herbicide Resistance Conference Feb 2013....................................................................................................................... 157 7 IDA10447: Industry Development Award - AgriKnowHow - Precision Agriculture (PA) and Information and Communication Technology (ICT) study tour ................................................ 158 IDA10463: Industry Development Award - DAN - Understanding and interpreting grains research and extension ................................................................................................................ 160 IDA10482: Industry Development Award - Riverine Plains - Resistance Roadtrip...................... 161 IDA10503: Industry Development Award - Morowa Farm Improvement Group - Global Herbicide Resistance Challenge Conference .................................................................................... 162 IDA10508: Industry Development Award - Holmes Farm Consulting - Pulse market and agronomic opportunities .................................................................................................................. 163 IDA10513: Industry Development Award - Hooper Consulting - North America farmers study tour .................................................................................................................................... 164 IDA10587: Industry Development Award - WAGG in China - Continuous improvement and innovation of grower education and market development within the complex agricultural relationships between China and Western Australia. ............................................................ 165 IDA10588: Industry Development Award - Mallee Sustainable Farming Karoonda Field Day 2013 .................................................................................................................................... 166 IDA10589: Industry Development Award - South Australian grain production with declining rainfall tour .............................................................................................................................. 167 IDA10596: Industry Development Award - Learning the best from the West - FarmLink WA Study Tour ............................................................................................................................. 168 IDA10601: Industry Development Award - The Changing Nature of Agricultural Training ........... 169 IDA10614: Industry Development Award - Farming systems and the move to continuous cropping .................................................................................................................................... 170 IDA10621: Industry Development Award - Grower attendance - Australian Summer Grains Conference June 2013 .................................................................................................... 171 IDA10728: RPI New Zealand Study Tour............................................................................ 172 IDA10732: IDA - WANTFA - Growers South America Study Tour ................................. 173 IDA10734: IDA - RAIN - Perth 2014 Agribusiness Crop Updates ............................................ 174 IDA10736: IDA - MSF - Mallee Sustainable Farming team participating in the GRDC Update for Advisors - South Australia................................................................................................ 175 IDA10740: IDA - ASS - Study tour of research and farming practices that improve soil management in the low rainfall Mallee................................................................................................... 176 IDA10741: IDA - Rural Directions - Study Tour to WA Northern Wheatbelt to look at Herbicide Resistance and Other Issues ............................................................................................ 177 IDA10743: IDA - Ag Excellence Alliance - Annual Grower Group Forum.................................. 178 IDA10772: IDA - UNFS - Eastern Low Rainfall Zone Bus Tour............................................... 179 IDA10784: IDA - BCG - Grower study tour of WA Northern Agricultural Region focused on management of resistant wild radish.................................................................................. 180 IDA10798: IDA- RAIN - 2014 Innovation Generation Conference - Toowoomba, Queensland..... 181 IDA10799: IDA - Liebe Group - Innovation Generation Young Farmers Tour ............................ 182 LYH00001: Theme 6 Strategy and MERI Plan..................................................................... 183 ORM00011: Farm Business Management Updates in the Southern Region ............................. 184 6. Building Skills & Capacity IDA10452: Industry Development Award - Dodgshun Medlin Agricultural Management - Ten Years of No-till in the Victorian Mallee "Celebration and Demonstration" .......................................... 159 PIG00007: Building the human capacity of grain growers through Partners in Grain.................. 186 SEP00012: Technology integration workshops in the Western Region.................................... 187 TAY00002: GRDC Network Stakeholder Engagement Strategy ............................................. 188 TEK00001: Western Region Fast Track- Determining economic rates and incorporation for lime in the Eastern wheatbelt o ................................................................................................... 189 UHS10054: Undergraduate Honours Scholarship - Julian Greenwood (ANU) Genetic regulation of cereal spike architecture .................................................................................................. 190 UHS10225: Undergraduate Honours Scholarship - Bethany Clark (UM) Leptosphaeria effectors: key determinants of blackleg disease of canola ................................................................... 191 UHS10226: Undergraduate Honours Scholarship - Cheryl Day (UWA) Economic impact of soil borne diseases over the entire rotation sequence ................................................................ 192 UHS10228: Undergraduate Honours Scholarship - Deon Heys (UT) Effect of irrigation and N supply on water-use efficiency of barley ....................................................................................... 193 UHS10229: Undergraduate Honours Scholarship - Elisha Thynne (ANU) Analysing the production risks in the white grain pathogen Botryosphaeria zeae.......................................................... 194 UHS10232: Undergraduate Honours Scholarship - James Campbell (UWA) Effect and economic benefit of rate of soil wetting agent in gravelly loam pasture and cropping systems. .................. 195 UHS10234: Undergraduate Honours Scholarship - Jasmine Hart (UF) Interactions of actinobacteria with rhizobia................................................................................................................... 196 UHS10235: Undergraduate Honours Scholarship - Jeremy Brown (US) In crop assessment of soil microbial communities following compost application ........................................................... 197 UHS10236: Undergraduate Honours Scholarship - Joanna Lang (UWA) A financial analysis of onfarm grain storage and handling in Western Australia........................................................... 198 UHS10237: Undergraduate Honours Scholarship - Karen Shears (UQ) A quantitative approach relating crown-rot resistance to water-use efficiency and grain yield in wheat (Triticum aestivum)199 UHS10238: Undergraduate Honours Scholarship - Kimberley Duver (UNE) Application of mixed waste organic outputs to cropping soils - potentialbenefits and pitfalls: impacts on soil biology and function" ........................................................................................................................ 200 6. Building Skills & Capacity UHS10241: Undergraduate Honours Scholarship - Marcela Cespedes (USA) Image fusion for phenotypic analysis of plant images................................................................................... 201 8 UHS10242: Undergraduate Honours Scholarship - Melissa Ann Coventry (UA) Can rye genes be used to ‘supercharge’ aluminium tolerance in wheat and barley? - A transgenic approach to dissect biochemical and biological properties of organic anion transporters from rye a ......................... 202 UHS10244: Undergraduate Honours Scholarship - Oliver Mead (ANU) An analysis of gammaaminobutyric acid metabolism in the wheat pathogen Stagonospora nodorum.......................... 203 UHS10338: Undergraduate Honours Scholarship - Nadine Nolan (QUT) Immigration levels of C.ferrugineus ................................................................................................................. 204 UHS10356: Undergraduate Honours Scholarship - Michelle Low (UCS) PBA Health Benefits of Australian Pulses............................................................................................................ 205 UHS10360: Undergraduate Honours Scholarship - Kyle Reynolds (UCS) PBA Health Benefits of Australian Pulses............................................................................................................ 206 UHS10403: Undergraduate Honours Scholarship - Brenton Leske (UWA) Reducing the impact of aluminium toxicity using superposphate as a seeding fertiliser ............................................... 207 UHS10410: Undergraduate Honours Scholarship - Kimberley Adams (UWA) Improving crop establishment in non-wetting soils through stubble management............................................ 208 UHS10411: Undergraduate Honours Scholarship - Liam Ryan (UWA) Monitoring the water status of wheat using novel magnetic probes ................................................................................... 209 UHS10416: Undergraduate Honours Scholarship - Wesley Lefroy (UWA) Does knowledge of the soil map improve the utility of remotely sensed soil information? ............................................ 210 UHS10418: Undergraduate Honours Scholarship - Benjamin Schreiber (UNE) Signalling and Nutrient Partitioning during Sorghum Grain-fill Under Stress: Informing Breeding Strategies....... 211 UHS10444: Undergraduate Honours Scholarship - Adam Norman (UA) A genetic dissection of physical grain quality in wheat .......................................................................................... 212 9 UHS10446: Undergraduate Honours Scholarship - King Yin Lui (CUR) Dissection of genetic factors affecting Ascochyta lentis pathogenicity and its interaction with lentil (Lens culinaris) ................ 213 UHS10456: Undergraduate Honours Scholarship - Bradley Harding (Uni Sunshine Coast) Development of molluscan contraceptives: Investigation into the molecular basis of reproduction in invasive crop snails and slugs........................................................................................... 215 UHS10457: Undergraduate Honours Scholarship - Clare Flakelar (CSU) Components in Canola Oil .................................................................................................................................... 216 UHS10458: Undergraduate Honours Scholarship - David Zadow (UWA) Grain Profitability maps for Western Australia ........................................................................................................... 217 UHS10469: Undergraduate Honours Scholarship - James Hughes (UW) Improved methods for the analysis of disease ratings, with application to the GRDC funded Crown Rot project ................. 218 UHS10479: Undergraduate Honours Scholarship - Deborah Bud (UW) Improved Canola Variety Information for Farmers ................................................................................................... 219 UHS10480: Undergraduate Honours Scholarship - Stefan Schmitt (UA) Genetic Control of Heat Tolerance in Wheat ......................................................................................................... 220 UHS10485: Undergraduate Honours Scholarship - Daniel Ahfock (UQ) Quantifying uncertainty in genetic map construction ................................................................................................. 221 UHS10488: Undergraduate Honours Scholarship - Huw Ogilvie (ANU) Optimising root architecture for yield and environmental benefits: identifying genetic and epigenetic targets by manipulating peptide relayed signals .................................................................................................... 222 UHS10504: Undergraduate Honours Scholarship - Mark Turner (UQ) RNA signalling and plant disease resistance.......................................................................................................... 223 UHS10509: Undergraduate Honours Scholarship - Sandra Whitmarsh (UA) Analysis and performance of key perennial forage shrub to improve production and sustainability in the mixed farming systems of the SA and Victorian Mallee .................................................................. 224 UHS10527: Undergraduate Honours Scholarship - Alexander Pocock (UQ) Discovery of small RNA in the wheat transcriptome ............................................................................................... 225 UHS10548: Undergraduate Honours Scholarship - Bonnie Hargreaves (UWA) Heritability of resistance to black spot disease in a field pea recurrent enrichment and association population (Pea-REAP)................................................................................................................... 226 UHS10549: Undergraduate Honours Scholarship - Jai Strempel (UF) Micro RNAs and other phloem molecules coupled with physiological recordings of differing wheat cultivars under salinity stress. .................................................................................................................................... 227 UHS10659: UHS - Candy Taylor (UWA) Understanding the role of floral integrator locus FT in controlling phenology in narrow-leafed lupin for future breeding ............................................. 228 UHS10662: UHS - Eleanor Readford (US) The effect of preceding rotation crop (wheat, mustard and chickpea) on the incidence of crown rot in wheat ........................................................... 229 UHS10663: UHS - Ashley Jacobs (UWA) Economic Analysis of the Harrington Seed Destructor 230 UHS10665: UHS - David Brunton (UT) Determining the residual effect of Roundup (glyphosate) on successful crop establishment .......................................................................................... 231 UHS10668: UHS - Belinda Worland (UQ) Gene expression of nitrate transporters in Sorghum bicolor (BTx623) under variable conditions of nitrate supply .................................................. 232 UHS10669: UHS - Danielle Button (UT) Thresholds of success - the on-farm economics of expanding irrigated grain production in Tasmania's midlands................................................. 233 6. Building Skills & Capacity UHS10454: Undergraduate Honours Scholarship - Benjamin Romeo (US) The effect of slow release nitrogen fertilisers on biomass production, crop nitrogen content, grain yield and grain protein concentration....................................................................................................... 214 UHS10670: UHS - Emily Lamberton (US) Effect of windrow burning on water efficiency during the fallow ............................................................................................................................ 234 UHS10671: UHS - James Walter (UA) Interactions between phosphorus nutrition and plant development .................................................................................................................. 235 UHS10672: UHS - Jared Murray (UWA) The biochemical mode of action for members of a new stream of potential herbicides ........................................................................................... 236 UHS10673: UHS - Kyle Mart (UWA) Screening for drought tolerance in wheat: Examining leaf osmotic potential as a predictor of turgor loss point .............................................................. 237 UHS10674: UHS - Lachlan Rainer (RMI) Effect of cultivar, growth region and malting conditions on the limit dextrinase activity and inhibition in Australian malting barley...................................... 238 UHS10675: UHS - Matthew Blanc (UQ) TALENs as a novel Sorghum bicolour transformation method ......................................................................................................................... 239 UHS10676: To characterise a subset of genes in Stagonospora nodorum and determine their role in causing disease .......................................................................................................... 240 UHS10677: UHS - Olivia Agar (US) Discovering genetic variation in pollen heat tolerance in wheat .................................................................................................................................... 241 UHS10678: UHS - Scott Thompson (UWA) Does the reflectance of visible and near infrared light differentiate between frosted and non-frosted wheat?........................................................... 242 UHS10679: UHS - Shona Wood (UQ) Field assessment of highly digestible sorghum starch biosynthetic pathway variants ........................................................................................... 243 UHS10681: UHS - William Nak (UQ) Pseudomonas infection response in NSP mutants............ 244 UHS10782: UHS - Bethany Radford (RMI) The effects of pre-processing on starch characteristics in cereal grain foods ........................................................................................................... 245 US00069: Enhancing human capacity for soil knowledge transfer and decision making for a sustainable grains industry ............................................................................................... 246 UT00029: Primary Industry Centre for Science Education (PICSE) - Phase 3 Extension............ 248 6. Building Skills & Capacity UWA00114: Capacity building in Production Agronomy and Farming System (teaching, research and postgraduate training) at UWA in partnership with GRDC ............................................... 249 10 Introduction Projects not listed in this review include: • service-type activities such as communication, publications, updates and workshops; conference sponsorships, training scholarships and travel and industry awards. • The review expands our existing communication products where we summarise the R&D portfolio in publications such as the Five-year Strategic Research and Development Plan, the Annual Operating Plan, the Annual Report and the Growers Report. GRDC’s project portfolio is dynamic with projects concluding and new projects commencing on a regular basis. The summaries provided in this review are for research projects active throughout 2013. The summary is presented under each of the GRDC’s six R&D investment themes. 1. 2. 3. 4. 5. 6. Meeting market requirements; Improving crop yield; Protecting your crop; Advancing profitable farming systems; Maintaining the resource base, and Building skills and capacity. Presenting it in this way also shows the progressive reshaping of the research portfolio to focus on the critical aspects of grain production in Australia. This review is a pilot of what is proposed to become a regular publication, available to everyone from the GRDC website. The GRDC values the input and feedback it receives from its stakeholders and so would welcome your feedback on any aspect of this first review. This way we can continue to improve and extend this summary To send us your feedback please email us at feedback@grdc,com,au Figure 1 provides an overview of the GRDC’s investment process; the following sections describe the six key steps in the process. 11 Step 1—Identification of priorities Throughout the year, the GRDC improves its understanding of grains industry RD&E priorities for the year ahead. Potential issues are identified through: • • • • interaction with growers, advisers and other industry participants, through Regional Cropping Solutions network activities such as grower updates, adviser updates, forums and field days consultation with the GRDC regional panels, GPA, researchers, state farming organisations and the national and regional agribusiness reference groups, evidence from project reviews, project progress reports and survey results, and consideration of the Australian Government’s National Research Priorities and Rural R&D Priorities. All priorities are looked at as potential areas for investment in future investment cycles. Initial suggested high-level resource allocation for the year ahead of the current financial year is reported to GPA, in the form of a draft stakeholder report, prior to December. Final resource allocations at the theme level are recommended to the GRDC Board in April of the following calendar year. Step 2—Investment Planning Week In July/August, GRDC managers and regional panel members meet to discuss issues and investment areas that address gaps in investment strategies and the priorities identified through engagement with stakeholders. GRDC managers then develop ‘mini prospectus’ proposals for new RD&E projects and existing projects that are due to be reviewed for further investment. The mini prospectus outlines the project’s aims, deliverables and approximate budgets. Each proposal is categorised as a national investment or a regional investment, and the appropriate procurement method (open tender, limited tender, multistage tender or direct negotiation) is identified. The proposals are ranked by GRDC managers, taking into account the relevant 6. Building Skills & Capacity This review of research and development projects supported by the Grains Research and Development Corporation during 2013 aims to improve awareness among growers, advisers and researchers of some of the these projects. Written in a friendly and industryorientated style by our research partners, the review is intended to communicate a summary of current research in a brief but useful way The GRDC Investment Process five-year strategic R&D plan. The GRDC’s National Panel makes recommendations for resource allocation to frame a high-level budget. Based on procurement recommendations made during Investment Planning Week, the annual external investment plan (for the next financial year) is formulated. Step 3—Call for tenders The external investment plan is released in August/September. Investment proposals identified as suitable for competitive tender are published in the plan, which is posted on the GRDC’s website during the period of call for tenders. Tenders are evaluated against specific selection criteria to determine the preferred provider(s). About half of the total new investments in any given year go to tender. Other projects are directly negotiated, particularly where there is limited expertise in a research area and/or there is a need for ongoing access to coowned intellectual property. The GRDC usually invests in partnership with the organisations that will deliver the RD&E. This means that most project investment is a combination of GRDC funding and research provider investment. Step 4—Contracting 6. Building Skills & Capacity The contracting of projects for the next financial year begins after the Board gives preliminary approval to theme allocations in August/September. Step 5—Status, progress and gap review meeting In February/March, GRDC managers and regional panel members meet to review and formalise the investments for the forthcoming financial year. During this meeting: • • • 12 • • Progress on the status and development of new investments agreed to during Investment Planning Week is reviewed. The resources being contributed to each project by the research partner(s) are assessed. Progress in the contracting of projects is reviewed. Actual investment that will occur in the next financial year is refined in line with the high-level budget. Investments and refinements to budget are reflected in the drafts of the annual operational plan and • stakeholder report. Strategic gaps are assessed, along with priorities for investment identified through engagement with stakeholders, to scope potential investments for later financial years. After the meeting, the National Panel provides revised theme allocations to the GRDC Board. These recommendations form the basis of the annual operational plan and the finalised stakeholder report. The annual operational plan must be submitted to the Minister for Agriculture, Fisheries and Forestry by 1 May. Approval from the Minister is required prior to the funding of projects. Step 6—Assessment of reports In March each year, annual progress reports on continuing investments are received and assessed by GRDC managers, in terms of both the project’s status against contract milestones and the quality of the report. The assessment process identifies issues for GRDC managers to follow up. Payments are made to research partners for projects that have good quality progress reports and are on track to meet milestones and achieve contract outputs. Final reports are assessed for quality of reporting and achievement of contract milestones and outputs at the end of each project (this usually occurs in September) 13 6. Building Skills & Capacity Figure 1 Overview of the investment process. (GPA = Grain Producers Australia, RCS = Regional Cropping Solutions) Theme 6 Building Skills and Capacity This theme is focused on generating leadership, innovation and education in the grains sector. Grains industry RD&E leadership and communication The GRDC invests in a range of activities, including: sponsorship for conferences and similar activities, the Australian rural leadership Foundation Program, Nuffield Australian Farming Scholarships, the Primary Industry Centre for Science Education and the National Youth Science Forum. Capacity building in the extension sector The GRDC supports opportunities to maintain or build knowledge and skills among extension providers. One important investment is support for Adviser Updates, which provide targeted information to advisers. Capacity building in the research and development sector The GRDC invests to increase the capacity of the R&D sector, including: • • • Grains Industry Research Scholarships (GRS) - to encourage post graduate training. Grains Industry Undergraduate Honours Scholarships (UHS) - to encourage training at undergraduate level. Travel Awards granted to researchers, individuals or groups wishing to attend a conference or undertake travel that aligns with the GRDC’s objectives and demonstrates direct benefit to the Australian grains industry. Capacity building for growers The GRDC supports training opportunities and resources to help growers access additional skills and new knowledge. • • Industry Development Awards (IDA’s) - for Australian growers or groups for study tours, travel or other forms of training or study activities approved by the GRDC Grower Updates – seminars that deliver the outcomes of GRDC-supported research, presented across all grain growing regions, sometimes in conjunction with grower groups. AAA00007: ABCA 2012-13 and 2013-14 15 GRDC Region Contract Start 01/07/2012 Contract End Organisation Project Code Agricultural Biotechnology Council of Australia Phone Project Summary Email AAA00007 6. Building Skills & Capacity Contact Mr. Claude Gauchat 30/06/2014 ARL00007: Australian Rural Leadership Foundation Limited GRDC Region Contract 01/07/2009 Start Contract End Contact Dr. Lesley Fitzpatrick Organisation 6. Building Skills & Capacity Project Summary 16 Project Code Australian Rural Leadership Program Phone 31/10/2013 Email ARL00007 ARL00008: Australian Rural Leadership Program 17 GRDC Region Contract End Contact Dr. Lesley Fitzpatrick Organisation Project Code Australian Rural Leadership Program Phone Project Summary 30/06/2015 Email ARL00008 6. Building Skills & Capacity Contract 01/07/2012 Start DAN00185: Post Doctoral Fellow - Soil nutritional chemistry in southern grains farming systems GRDC National Region Contract Start 07/01/2013 Contract End Contact Dr. Georgina Kelly Organisation Phone 30/06/2016 Project Code DAN00185 Department of Primary Industries, an office of the Department of Trade & Investm 02 8843 1121 Email [email protected] Project Summary 6. Building Skills & Capacity Australian grain crops were planted across 24 Mha in 2011. In 2011, Australian agriculture used a combined 1632 kt of N, P and K to manage soil nutritional constraints to production. NSW DPI is a key provider of research and development capacity and capability in relation to soil nutritional chemistry for the Australian grains industry. Dr Mark Conyers is a Principal Research Scientist of NSW DPI (Wagga Wagga) and has over 30 years experience in soil nutritional chemistry in southern grains farming systems. 18 Dr Conyers is nearing retirement and upon retirement will take a wealth of 'soft' knowledge with him that will severely reduce the capability and capacity in the field of soil nutritional chemistry for the GRDC southern farming systems region. Dr Conyers is one of several senior nutritional soil scientists around Australia in a similar situation, the retirement of which will substantially reduce the pool of expertise in this field for the entire Australian grains industry. This proposed investment will result in the appointment of a post-doctoral fellow that is jointly funded by GRDC and NSW DPI for the next 3 years (2013-2016) as an understudy to Dr Conyers and will result in a maintained capacity in the field of soil chemistry in relation to crop nutrition. Following the initial 3 year period of costsharing, NSW DPI will meet the on-going salary costs of this position. DAS00142: Traineeships in Applied Grains Research 19 GRDC Region National Contract End Contact Dr. Kathy Ophel-Keller Organisation Phone 31/12/2016 Project Code DAS00142 South Australian Research & Development Institute SARDI 08 8303 9368 Email [email protected] Project Summary The aim of this project is to encourage undergraduate agricultural science students into applied research in the grains industry. We are offering one year interships focusing on areas of key need, particularly in crop protection and agronomy. The students will have the opportunity to learn research skills, understand field research, communication to an industry as well as scientific audience and gain exposure to a range of research project areas. The specific objectives are:-To encourage high quality agricultural science students to pursue applied research as a career-To give students who are interested in applied R & D a broad overview of the fundamentals of applied R & D relevant to the grains industry. 6. Building Skills & Capacity Contract 01/01/2014 Start DER00018: GRDC Network Survey 2013 GRDC Region Contract 09/10/2013 Start Contract End Contact Organisation Project Code Down to Earth Research Phone 6. Building Skills & Capacity Project Summary 20 31/01/2014 Email DER00018 GCS10433: Improved Adaptation to Drought. 21 GRDC Region Contract End Contact Prof. William Erskine Organisation Phone 31/08/2013 Project Code GCS10433 University of Western Australia 08 64881903 Email [email protected] Project Summary The theme of the Master Class: Adaptation to Drought - is highly relevant to the grains industry in Australia and to that in developing countries. Master Classes are primarily aimed at mid-career agricultural scientists from developing countries, and are not an entry-level training. Each Class consists of from 12 - 20 participants. A special point is made of including a few Australians in Master Classes wherever possible. A filter to ensure quality of participants is in place whereby a pre-Master Class questionnaire is administered to potential participants to ensure the appropriateness of each individual’s experience and work context. For Australia the Master Class training will be highly relevant to junior to mid-career plant breeders and to up-skill experienced research officers in plant breeding programs. Of particular value to Australian breeders will be the knowledge sharing and interaction with other plant breeders practising in a wider range of Third-World contexts - this was our experience in the earlier Master Class on Collaborative Breeding (UWA00137). Australian plant breeding programs both public and private - and hence the Australian grains industry - will benefit from the training in the Master Class particularly through improved drought phenotyping. 6. Building Skills & Capacity Contract 27/08/2013 Start GCS10750: Conference Sponsorship - GrassRoots Agronomy for Women in Farm Business GRDC Region National Contract 15/04/2014 Start Contract End Contact . Louise Flohr Organisation 16/04/2014 Project Code GCS10750 Agrilink Agricultural Consultants Pty Ltd Phone Email [email protected] Project Summary The ultimate goal is to improve the skills, capacity and knowledge of women in farming businesses. 6. Building Skills & Capacity Women farming business partners are represented in low numbers at industy events, and this workshop will provide women with an opportunity to build on agronomic skills, that would otherwise be difficult to obtain. In many cases that I am aware of, the partner of grain buisnesses (i.e. wife) have come from an urban area or non-agricultural background, and knowledge about the day-to-day operations on farm is limited. They can also lack detailed knowledge of why certain 22 operations, such as weed control and disease management are important. If we can educate these women and empower them with the knowledge and skills, it will ultimately build towards a healthy farm business thus advancing towards more profitable farming systems. Women are traditionally the childcarers, and if we can get a positive message to them about their industry, hopefully the message will then be instilled within their children and encourage the next generation into the industry. GCS10751: Conference Sponsorship Extending life cycle assessment (LCA) to include soil Contract Start 04/08/2014 Contract End Contact . Marguerite Renouf 04/09/2014 Project Code GCS10751 Organisation Phone 0478 220551 Email [email protected] Project Summary The indicators commonly used in LCA do not represent the full scope of environmental impacts of relevance for agricultural industries. The recommendations from the recently completed AusAgLCI project (with investment from GRDC) identified ‘soil function’ as an important additional impact category to add to the national inventory. This workshop will be an important step towards adding soil functions and qualities as indicators represented in agricultural LCA studies. In doing so, it will arm the grains industry with a more complete picture of its environmental impacts and environmental improvement efforts, and help it fulfil its environmental stewardship responsibilities. The workshop will also further strengthen the constructive relationship between the grains industry (GRDC) and the LCA research community, so that developments in LCA, sustainability indicators and product labelling can be guided and moderated by industry insight. 6. Building Skills & Capacity GRDC National Region 23 GCS10752: Conference Sponsorship - BCG Grains Research Expo GRDC Region National Contract 07/02/2014 Start Contract End Contact . Ciara Cullen 07/03/2014 Project Code GCS10752 Organisation Phone (03) 5492 2787 Email [email protected] 6. Building Skills & Capacity Project Summary 24 The BCG Grains Research Expo is staged to give grain growers, researchers, farm consultants and other agricultural product and service providers a platform to engage with and learn from one another. Farmers take home messages and ideas that can be used to improve the productivity, profitability and sustainability of their farm enterprise. services through exhibition booths. The BCG Grains Research Expo is an event that is well regarded for its capacity to encourage farmerto-farmer learning, the dissemination of agricutlural research findings, a technical hub showcasing the latests apps and electronic communications and hands-on demonstrations. The event features concurrent presentations focused on agronomic and farm business management topics. Almost 100 industry service providers also attend the event annually to showcase their latest products and The Grains Research Expo is renowned for utilising grain growers time efficiently by having a plethora of information on hand on one day, in one place. GCS10754: Conference Sponsorship International Association of Plant Biotechnology 2014 25 Contract Start 08/10/2014 Contract End Contact . Josie Gruber 08/11/2014 Project Code GCS10754 Organisation Phone (02)91268042 Email [email protected] Project Summary The symposium, which convenes every four years, is the peak meeting of scientists involved in plant biotechnology. The opportunity to bring the meeting to Australia is highly prestigious for the national pre-breeding and breeding industries. Significant advances have been made during the period of the last meeting in a range of technologies and approaches for crop plant improvement, within and between a range of industries. The conference will provide a key forum for exchange of information and discussion of such issues, wth consequent benefit to breeding programs servicing the Australian grains industry. 6. Building Skills & Capacity GRDC National Region GCS10755: Conference Sponsorship Australasian Plant Breeding Conference 2014 GRDC Region National Contract 19/10/2014 Start Contract End Contact . Esther Price 22/10/2014 Project Code GCS10755 Organisation Phone (08)95259222 Email [email protected] Project Summary 6. Building Skills & Capacity The conference will be the first Australasian Plant Breeding Conference since 2009. Significant advances have been made within this time in a range of technologies and approaches for crop plant improvement, within and between a range of industries. The 26 conference will provide a key forum for exchange of information and discussion of such issues, with consequent benefits to breeding programs servicing the Australian grains industry. GCS10757: Conference Sponsorship Farmsafe - Working for the Future 27 GRDC Region National Contract End Contact . Kerri-Lynn Peachey 16/10/2014 Project Code GCS10757 Organisation Phone 02 6752 8210 Email [email protected] Project Summary There will be specific focus on implementing practical farm safety management. To match this broad representation of rural industry and service sectors, the conference will focus on improvements in safety standards through commodity-based injury prevention programs. As one of the major commodity sectors the grains industry stands to benefit from improved Work Health & Safety systems and a better understanding/appplication of these by producers. Specific information for growers to improve knowledge and understanding of various dangers and hazards (such as fatigue and effects of aging) to enhance behaviour changes will be provided. 6. Building Skills & Capacity Contract 15/10/2014 Start GCS10759: Conference Sponsorship - Sydney Royal Easter Show - Grains education displays GRDC Region National Contract 04/10/2014 Start Contract End Contact . Jodie Dean Organisation Phone 23/04/2014 Project Code GCS10759 Royal Agricultural & Horticultural Society 02 9704 1156 Email [email protected] Project Summary Similar to the benefits for GRDC directly, this proposal offers a unique opportunity for the Australian Grain industry to delivery consumer messaging and promotion about the industry to a vast and diverse audience. 6. Building Skills & Capacity The proposal of having two displays across the Sydney Royal Easter Show allows the messages to be tailored to the different audiences at the Sydney Royal Easter Show. 28 The Centre of Agricultural Excellence, located alongside the Woolworths Fresh Food Dome reaches will be focused on messaging applicable to the general consumer along with education messages applicable to high school, tertiary and vocational education levels. The RAS operated a comprehensive Schools Program at the Show attracting 12,000 students in 2013. Approx 60% were high school students, predominately studying subjects including agriculture, food technology and design technology. The key messages of this Schools Program are: Innovation and Technology in Australian Agriculture and Career opportunities. So as to appropriately reach this audience, the display and messaging will be around; innovation and technology, excellence in Australian agriculture, understaing of grains and products, the breadth of the Australian industry and our place in the global market, nutrition and grain to end use matching and sustainability. The Centre for Agricultural Excellence displays the grains entries to the Sydney Royal compeitions, as received by the iconoc District Exhibits so this display would deliver supplimentry information and education around this display. The Food Farm pavilion offers a different but similarly important opportunity, to connect with young children about food and agriculture messaging. The Food Farm, ideally located along the Animal Walk (a colourful pathway driving showgoers through the agricultural precinct) is targetted at pre and primary aged children. Families with young children are the largest deomographic attending the Show each year and is suplimented by School Groups and vacation care groups. The Food Farm offers a hands on, interactive learning for children, highlighting the 'Paddock to Plate' message through the pavilion themeing. All of the interactive activities pose to answer the question "Where does our food come from" and highlights the role of Australian farmers in producing it. There has been an effort to increase the presence of the grains industry message in this pavilion over the past two years and there are now displays where children are able to mill wheat grain, roll oats and roll out pastry. There is also a tractor in the pavilion that children are able to climb into and examples of a range of grains. We hope to expand this to show a greater range of grain products, on farm storage and a more complete message of the process from the paddock to the plate. GCS10760: Conference Sponsorship - SAGA 2014 Industry Day - Life beyond Glyphosate 29 GRDC Region National Contract End Contact . David Georg 28/02/2014 Project Code GCS10760 Organisation Phone 08 8389 1856 Email [email protected] Project Summary Awareness of the current state of increasing glyphosate resistance. An opportunity to learn about strategies to prevent/delay the development of glyphosate resistance in corridors such as fencelines, roadside, etc Discussion of strategies to minimise the transfer of resistant weeds from roadsides to paddocks where grain crops are grown. Planning alternative weed management strategies for the time when glyplosate is no longer effective. 6. Building Skills & Capacity Contract 27/02/2014 Start GCS10761: Conference Sponsorship - 2014 SANTFA Conference GRDC Region National Contract 21/02/2014 Start Contract End Contact . Alexandra Milner-Smyth 22/02/2014 Project Code GCS10761 Organisation Phone 08 8842 4278 Email [email protected] 6. Building Skills & Capacity Project Summary 30 The SANTFA conference focuses on communicating the benefits of conservation agriculture principles direct to SA cropping farmers. aim of encourageing local farmers to adopt contemporary farming methods and technologies which seek to increase gross profit, system resiliance and ecological health. Expert speakers, including leading farmers, deliver information on topics such as controlled traffic, stubble retention, minimum tillage, cover cropping and seed singulisation with the The SANTFA conference would not be possible without the generous and ongoing support of the GRDC. GCS10762: Conference Sponsorship - SEPWA Harvest Review and Season Preview, 2014 31 GRDC Region National Contract End Contact . Niki Curtis 15/03/2014 Project Code GCS10762 Organisation Phone Email Project Summary The grains industry benefit from SEPWA's annual harvest debrief for the following reasons: valuable extension opportunity of new crop varieties and crop management practices; networking opportunity for everyone in the industry; a great way to find out first hand the results of the SEPWA on farm farmer variety trials; and linkages to growers, marketers, researchers and international end users are fostered on the day 6. Building Skills & Capacity Contract 14/03/2014 Start GCS10764: Conference Sponsorship Tasmanian Farmers & Graziers Association 2014 Major Conference GRDC National Region Contract Start 07/03/2014 Contract End Contact . Nardia Deverell 07/04/2014 Project Code GCS10764 Organisation Phone (03) 6332 1800 Email [email protected] 6. Building Skills & Capacity Project Summary 32 The theme of the 2014 Tasmanian Farmers and Graziers Association’s biennial conference is Taking Responsibility: farmers and those in our agribusinesses taking it upon themselves to determine their future. The conference will be held on 3-4 July 2014 at the Country Club Tasmania, Launceston. The conference theme derives from the strategy we have set ourselves as an industry, not just the TFGA, to optimise our position in the Tasmanian economy through to the year 2025 and beyond, but using 2025 as a milestone date. We have adopted what we call a Team Agriculture approach (TeamAg). It is unashamedly ‘all for one and one for all’. By thinking, planning and working as a team, we can achieve our potential and our visions. Farming, like no other enterprise, is a legacy business. We have to pass on to the generations that follow us land that they can continue to use and develop in perpetuity. That requires sustainable management. We all adhere to the belief that we have to pass on our land and enterprise in a better state than when it came into our hands. That is the end game of us taking responsibility, acting responsibly. In our 2025 strategic plan we have committed ourselves to six central beliefs: 1. We will be responsible for our performance and behaviour as an industry; 2. We will position and drive a positive image and view of Tasmanian agriculture; 3. We will attract and develop people working in agriculture; 4. We will work to improve the operating environment to conduct the business of agriculture; 5. We will work to deliver infrastructure improvements to expand agriculture; 6. We will work to deliver faster industry improvements from research and technology and innovation. What that means: We are working as a united industry to lift our game in understanding the size, structure, segmentation and trends in the industry and implications of that for the future. We will present a positive, professional and united front to government, the media and the community. We will state our case clearly and unequivocally to government and those that influence government policy. That entails us gathering and maintaining better data and baseline information and monitoring and measuring performance against agreed benchmarks. Presenting a positive image of Tasmanian agriculture means we must first understand both our audience and our selling points. Through such measures as focus groups, we can appreciate what the public perception of agriculture is. We might assume that the farmer is everybody’s hero, but it ain’t necessarily so. Without public understanding of what we do and how we do it, we cannot expect public support when we need it. We are taking responsibility for that. Attracting people, especially young, qualified people, into the business of agriculture is challenging. Perhaps there is still a view that it involves hard, back-breaking work whereas, as we all know, farming is now highly mechanised, extensively computerised and as much a white collar profession as a blue collar trade. The TeamAg approach of determining the ‘we will’ priorities is a clear demonstration of those in this industry taking responsibility for it into the future. Therefore, the Taking Responsibility theme for the 2014 conference is both timely and appropriate. 33 6. Building Skills & Capacity Therefore, the task is to promote a career in Tasmanian agriculture as a valuable and wise choice, and an interesting and multi-faceted career that offers the best of both worlds. That, in turn, involves us taking responsibility for the path of agricultural education. We must engage with education institutions and educators to influence what people are taught. Farmers have never been reluctant advocates for change, but the art of advocacy itself is changing. Communication is instant, access to information is immediate. That places legislators under greater pressure than ever before. We are not the only ones banging on their door. One of the implications of that is the importance of the agricultural sector speaking to governments with one voice; it makes life a lot easier all round. GCS10765: Conference Sponsorship - 17th Australian Nitrogen Fixation Conference GRDC Region National Contract 28/09/2014 Start Contract End Contact . Matthew Denton 10/02/2014 Project Code GCS10765 Organisation Phone 08 8313 1098 Email [email protected] Project Summary Nitrogen fixation contributes 5 million tonnes of nitrogen to the Australian grains industry per annum sustaining cereal and oilseed production and reducing the reliance upon nitrogenous fertilisers. This conference aims to increase the contribution of nitrogen from pulse and pasture legumes through greater understanding of inoculation and nitrogen fixation from legumes. It will also look at a variety of environmental aspects of nitrogen fixation. 6. Building Skills & Capacity GRDC sponsorship will enable the Conference Organizing Committee to invite keynote 34 speakers who will bring the latest scientific results and research directions as well as recent agricultural extension developments, particularly from North America and Europe. The interactions among Australian nitrgoen fixation researchers, and, in particular, the input from specifically selected overseas expert researchers will challenge Australian nitrogen fixation research to lead to greater understanding and contribution of nitrogen fixation, resulting in greater impacts and benefits of nitrogen fixed on Australian farms. GCS10766: Conference Sponsorship - 2014 International Plant Cell Wall Meeting 35 GRDC Region National Contract End Contact . Rachel Burton 31/07/2014 Project Code GCS10766 Organisation Phone 08 83131057 Email [email protected] Project Summary Major new drivers of international research in plant science include the roles of cell walls as renewable sources of transport fuels, as functional foods to improve human health, and as a source of raw materials for industrial processes. Production of these new products and adding value to existing crop residues can be achieved in farming systems without impacting on use the of agricultural land for food production. The ARC Centre of Excellence in Plant Cell Walls has built an international team with scale and focus at the scientific, technical and training levels to generate outcomes that will significantly enhance biotechnologies that underpin Australian crop industries valued at over $8 billion per annum, associated food industries valued at about $40 billion per annum, and massive emerging industries related to renewable transport fuels and biomaterials. It is expected that many international delegates will use their conference travel to Australia to visit University and Government research institutions in order to extend collaboration potential around the country. ARC Centre and other Australian researcher exposure to the breadth of expertise invited to and/or attending the conference will enhance existing strengths in the field of fundamental cell wall biology to generate scale and focus at the scientific, technical and training levels, to significantly enhance plant biotechnologies that underpin Australian crop industries, associated food industries and massive emerging industries related to the production of renewable transport fuels 6. Building Skills & Capacity Contract 27/07/2014 Start GCS10767: Conference Sponsorship - 8th Australasian Soilborne Diseases Symposium (8ASDS) GRDC National Region Contract Start 11/10/2014 Contract End Contact . Robert Tegg 13/11/2014 Project Code GCS10767 Organisation Phone (03) 6233 6830 Email [email protected] Project Summary ASDS8 will provide an opportunity to bring a range of international and national researchers together who conduct soilborne disease research for grain crops - bringing benefit to Australian grain growers. Also, young scientists (PhD students) attending will gain exposure to key researchers, both working in grains or the broader discipline, benefiting and learning from the interaction involved. 6. Building Skills & Capacity These skills gained can be passed onto the 36 grain industry in the future - providing longterm benefits. Additionally, this conference will provide a focus from which to develop collaboration among Australian scientists and for those involved in other GRDC projects to meet and progress existing collaborations and develop ideas for new, improved projects. GCS10768: Conference Sponsorship - 11th Australasian Plant Virology Workshop 37 GRDC Region National Contract End Contact . Sally Brown 15/08/2014 Project Code GCS10768 Organisation Phone 07 3201 2808 Email [email protected] Project Summary Plant viruses pose some of the greatest threats to grain crops in Australia, for example wheat (Wheat streak mosaic virus and High Plains virus), maize and sorghum (Johnsongrass mosaic virus), barley (barley yellow dwarf virus complex) and pulse legumes (cucumber mosaic virus, bean yellow mosaic virus, bean common mosaic virus, alfalfa mosaic virus, luteoviruses and geminiviruses). The Australasian Plant Virology Workshop is the pre-eminent conference in Australasia for plant virology and normally attracts representatives of all of the major research groups in this region, as well as a selection of international experts. As such, the workshop provides an ideal venue for researchers working on plant viral diseases of grains crops to exchange ideas, present the results of their projects, obtain critical feedback, develop new collaborations, and gain an appreciation of emerging issues in grains pathology. 6. Building Skills & Capacity Contract 13/08/2014 Start GCS10769: Conference Sponsorship - Digital Rural Futures Conference GRDC Region National Contract 25/06/2014 Start Contract End Contact . Marisa Parker 27/06/2014 Project Code GCS10769 Organisation Phone (07) 46312280 Email [email protected] Project Summary 6. Building Skills & Capacity As nominated by GRDC Panels in conjunction with Grains Council of Australia, 75% of the sponsorship will fund attendance of up to 4 young agricultural grower innovators, aged between 20 and 35: 2 x northern panel, 1 x southern panel, 1 x western panel. 38 The Digital Rural Futures Conference will act as a catalyst for discussion on some specific drivers of the grains industry e.g. total factor productivity (TFP) growth including improved agronomy through new digital systems and technologies, more efficient equipment, and improved business skills and decision-making, all combined to yield grower innovation. In recent years, the grain industry’s rate of growth in TFP - the ratio of the total quantity of outputs to total inputs has slowed, in part, reflecting the impact of a prolonged drought. Regaining the momentum in productivity growth, especially as a key response to the continuing decline in grain growers? terms of trade, is a high priority for the agriculture and grains industry. Additionally, during the Conference proceedings, there is the potential to promote the work of GRDC and its key research partners. As this conference is an initiative of the Regional Universities Network (RUN) partnership http://www.run.edu.au/cb_pages/member_univ ersities.php. - a diverse number of researchers from different institutions will be available. Current agricultural research projects are being undertaken in USQ?s Centre for Systems Biology (CSBi) and its National Centre for Engineering in Agriculture (NCEA), the Collaborative Research Network projects (Web based discussion support systems for Australian farmers and Enhanced practice through connectivity to digital technologies and information) as well as the UNE Smart Farms project . More information on current USQ research that will have direct impact on farming and/or the grains industry can be provided. GCS10771: Conference Sponsorship Victorian Farmers Federation Annual Grains Conference Contract Start 23/03/2014 Contract End Contact . Jayne Gleed 25/03/2014 Project Code GCS10771 Organisation Phone 03 9207 5538 Email [email protected] Project Summary By sponsoring the VFF Grains Conference GRDC is promoting a number of benefits to the Australian Grains industry. The Conference facilitates education, development, information exchange, research and extension, across all elements of the supply chain in the Grains Industry. For example, from varietal development, plant breeders, researchers, growers, input suppliers, financiers, marketers, extension services & advisors, transporters, customers, and government departments/regulators - to name a few. The requirement for such an event has only increased as the industry evolves and grain growers contend to keep pace with an ever evolving and more complex and sophisticated environment. Issues abound from production management (such as varietal selection, plant health, soil health, chemicals & fertilizer inputs, cropping practices, etc); finance management; physical grain management (such as harvesting, segregation, on-farm storage, quality & hygiene, grain treatment, transport, etc); through to marketing & risk management options (warehousing, cash, pools, contracts, options, etc). Thus the benefits of the event itself are derived by all participants in the industry, but most significantly to the benefit of the grain producers. Similarly, the event will assist GRDC by enabling it to both provide a major extension event direct to its major stakeholders (the producers), and will also enable GRDC to 'close the loop' as far as stakeholder (producer) feed back to GRDC as to the effectiveness and furture activities of GRDC. This can be achieved through both formal facilitated workshops, as well as informal participation and discussion between GRDC staff and regional panel members with the growers themselves. The VFF Grains Conference would be the primary event of its type in Victoria with such large numbers of grain producers attending and being focussed primarily on grain production across all regions. 6. Building Skills & Capacity GRDC National Region 39 GCS10774: Conference Sponsorship - AGSA AGSA2014 GRDC Region National Contract 25/08/2014 Start Contract End Contact . Mike Gidley 27/08/2014 Project Code GCS10774 Organisation Phone 0733652145 Email [email protected] Project Summary 6. Building Skills & Capacity Sponsorship at the platinum level will enable the organising committee to set the early bird registration fee at $600, and for students at $350. This makes the registration fee very competitive relative to the international conferences on grain science, and therefore is expected to encourage a large national 40 attendance of delegates from industry, academia, producers and students enabling the industry to maintain long-term competitiveness through the development and application of regionally relevant scientific knowledge. GCS10778: Conference Sponsorship - UNFS Annual Field Day 41 GRDC Region National Contract End Contact . Joe Koch 27/09/2014 Project Code GCS10778 Organisation Phone 0428672161 Email [email protected] Project Summary There are very few Grain Grower focussed Field Day Events held in the Upper North of South Australia. We would like to see the capacity and reach of this field day grow, but can not do that on our current operating budget for this event. By increasing our advertising and capacity for attendees the information and hands-on approach of the group will be able to reach a broader group of Grain Growers across the Upper North of South Australia, exposing them to new technologies, adapted land management systems for the difficult conditions faced across this district and a support network of adopters and innovators. 6. Building Skills & Capacity Contract 26/09/2014 Start GCS10789: Conference Sponsorship - NCMC Boosting Productivity in North-West NSW GRDC Region National Contract 21/05/2014 Start Contract End Contact . Jeremy Cape 22/05/2014 Project Code GCS10789 Organisation Phone 02 6686 5052 Email [email protected] Project Summary Recent research commissioned by the Australian Farm Institute indicates that productivity growth in agriculture, including in the grains industry, has stalled over the past ten years, which is in marked contrast to the previous 50 years. This is of concern as Australian farmers have relied on productivity growth to help maintain profitability. 6. Building Skills & Capacity Grain growers will be one of the key groups targetted by the conference, along with cotton growers, irrigators and cattle producers. Its aim will be to identify practical strategies for improving productivity. Of particular relevance to grain growers will be sessions on: plant breeding, nutrition and pest management, natural resource management, alternative energy, water and machinery systems and technologies, and enhanced agricultural labour 42 training, education and skills services and rural extension activities. The Namoi Catchment Management Authority is in transition to become the North West Local Land Service (NWLLS) which will combine the NRM responsibilities of the Namoi CMA with the agricultural extension activities of NSW DPI. The new organisation, which begins in January 2014, wishes to involve producers from different industries in north-west NSW to provide a unique and valuable opportunity for producers to learn from what others are doing and to contribute to identifiying practical strategies for boosting productivity. GCS10790: Conference Sponsorship - MSF Research, Development & Extension Tri-State Forum Contract Start 19/02/2014 Contract End Contact . Stephanie Haw 20/02/2014 Project Code GCS10790 Organisation Phone 03 5021 9100 Email [email protected] Project Summary The grains industry will benefit from the annual MSF Tri-State RD&E Forum for the following reasons: current and relevant information will be presented at this event from a Mallee perspective; men and women will be inspired and motivated to improve the way in which they operate within the Mallee to help farmers increase their skills and knowledge of building successful farm businesses; a networking opportunity for everyone who will attend; and linkages to growers, marketers, researchers and media will be fostered during the event. 6. Building Skills & Capacity GRDC National Region 43 GCS10797: Conference Sponsorship – WA Farmers 2014 Annual Conference - Grains Section GRDC National Region Contract Start 19/03/2014 Contract End Contact . Megan Macneill 20/03/2014 Project Code GCS10797 Organisation Phone 08 9486 2100 Email [email protected] Project Summary 6. Building Skills & Capacity The theme of the WAFarmers Annual Conference for 2013 is; "The Sustainability of the Industry'. There are continual threats to our industry and the purpose of WAFarmers 2014 Annual Conference is to give attendees the tools and information they need for a successful future. We want to ensure they maximise opportunities and minimise risk. 44 With this in mind and with the knowledge that GRDC is responsible for planning, investing and overseeing research and development, delivering improvements in production, sustainability and profitability across the Australian grains industry, it is only fitting that we provide our members with the opportunity to hear more about the future of the industry from GRDC. By way of sharing knowledge, innovation and developing a support network of key industry bodies who represent the future of the grains industry, we can provide a positive outlook for the future of Agriculture. The WAFarmers Conference is the primary policy making event for WAFarmers. By sponsoring the event, GRDC makes it possible for policy making input from growers across Western Australia on key grains issues along with other topics such as climate change, biosecurity and farm economics. GRDC represenatives can be part of these discussions.. WAFarmers is Western Australia's largest and most influential rural lobby group. Representing more than 4,000 farmers, WAFarmers is proud to be a member driven organisation. We are WA's only member of the National Farmer's Federation. Member input is essential to provide WAFarmers staff and elected members with clear policy direction. GGA00003: Grain Gain--Leadership for Grains Industry Innovation 45 GRDC Region National Contract End Contact Prof. Gordon MacAulay Organisation Phone 30/06/2013 Project Code GGA00003 Grain Growers Ltd 02 9888 9600 Email [email protected] Project Summary Grain Gain" consists of three training courses. These will provide tools to build leadership and representational skills for innovative grain producers and grains industry professionals and encourage the adoption of new practices. Leadership will be taught in the context of the grains industry value chain and the building of stronger farm businesses. Grain Gain: On Farm will provide high impact training designed to equip grain producers with technical knowledge and leadership tools that lead to more profitable post harvest management. Grain Gain: Technology will provide an understanding of the grains industry value chain so as to support the management of wheat quality on farm. Detailed information on the value chain and the technologies involved will be given so as to provide the technical foundation for the development of industry leaders with strong technical skills. This will include gaining an understanding of grain processing and grain products as a part of a value chain. Grain Gain: Markets will provide, in the context of developing leadership skills, a detailed understanding of the structure of domestic and international grain markets focussed on supplying grain into those markets. The content will include understanding leadership, domestic and international trade policies, industry and trade data and its analysis, product requirements and issues involved in developing a business around domestic and international trade. 6. Building Skills & Capacity Contract 01/06/2011 Start GRS10004: Grains Industry Research Scholarship - Alice Burgess (ANU) Identifying novel epigenetic components that regulate seed size in a model plant, Arabidopsis. GRDC National Region Contract 28/02/2011 Start Contract End Contact Dr. Ian Searle Organisation Phone 03/02/2014 Project Code GRS10004 Australian National University 02 6125 2322 Email [email protected] Project Summary The broad aim of my PhD research is to define a novel epigenetic mechanism that regulates seed size in plants. 6. Building Skills & Capacity We understand almost nothing about how plants regulate seed size, more specifically endosperm size. One mechanism influencing endosperm size is genomic imprinting, that is deferentially gene expression of the maternal and paternal alleles. Recently 5000 noncoding heterochromatic small interfering RNA (siRNA) producing loci were discovered to be 46 are paternally imprinted. These 5000 loci produce about 100,000 small RNAs. This is a unique and unappreciated level of gene regulation that acts at the posttranscriptional and transcriptional level that I aim to investigate. I am undertaking this project as seed size is an important determinate of grain yield, export revenue for Australia and global food security. GRDC National Region Contract 31/03/2011 Start Contract End Contact Dr. Ann McNeill Organisation Phone 30/03/2014 Project Code GRS10026 University of Adelaide 08 8303 8108 Email [email protected] Project Summary Quantitative analysis of the contribution of break crop root residues to P and N cycling and nutrient benefits to subsequently grown wheat Proposed supervisors: Senior Lecturer Dr. Ann McNeill and Professor Mike McLaughlin School of Agriculture, Food & Wine Background and Rationale for Proposed Research: Fertiliser phosphorus is one of the major nutrients in agriculture systems but is becoming an extremely limited resource and expensive (FIFA 2008; Van Raij et al. 2002). Paradoxically in systems where soluble P is present in excess it can cause serious environmental implications including pollution of waterways (Kirkegaard et al. 2008). Clearly the management of P is the key to sustainable agriculture systems and needs to be based on a sound understanding of the plant-soil P cycle. It has been reported that fertiliser P supplies only 20% of crop requirements with the remainder coming from cycling of organic inputs including plant residues (shoots and roots). It is well known that oil seed and grain legume break crops provide many benefits for the growth of following cereals including improved nutrient supply, particularly N (Evans et al. 2001). However, knowledge of P supply from residues is far less advanced than for N where the contribution from residues, both shoots and roots, has been well quantified (Evans et al. 2003). Whilst there is information concerning the effect that decomposition of immature shoot residue material has on available P in soil (Iqbal 2009) and uptake by succeeding plants (Hocking and Randall 2001) there is almost no data for mature root material despite the fact that roots may comprise up to 50% of the crop dry matter (ref). Furthermore the roots of certain break crops have been shown to mobilise P in soil via exudates (Nuruzzaman et al. 2005)) yet there is no understanding of how this might influence P supply to a following cereal crop. Using state of the art techniques ( including 32/33P tracers and HPLC analysis of root exudates) this study will (a) measure the direct effects of break crop roots on P solubilisation and whether this affects P nutrition in following wheat crops and (b) directly trace the release of P from mature break crop root residues. This information will allow farmers to assign a P ‘supply value’ to their break crops and enable them to make more economically and environmentally sounds decisions regarding applications of P fertiliser. Aim: Overall aim of this proposed research is to examine how certain break crops improve the nutrition of following wheat crops. There are two hypotheses to test of this research: 1. P mobilization by exudates of the break crop improves P nutrition of the succeeding wheat crop 2. Decomposition of break crop root 47 6. Building Skills & Capacity GRS10026: Grains Industry Research Scholarship - Foyjunnessa (UA) Assesing management options for enhancing soil phosphorus availability using rotations residues affect P nutrition of the succeeding wheat Proposed Research Program +1 year Major review of program This research will consist of a series of pot and glasshouse experiments over two years and field experiments in South Australia in the last year. +2 years Annual review of program Work Plan - milestones of structured program Preparation of final manuscript and submitted to the University of Adelaide February to April 2011 (+ 3 months) Draft of review, research proposal and initial seminar February 2011 and will be continued until the structured research program writing Review of literature June 2011 Commencement of the experiments July 2013 Completion of the experiments 6. Building Skills & Capacity July 2011 - June 2013 Testing innovative 48 techniques, Lab analysis, data analysis and writing + 3 years Annual review (structured program) October - December 2013 Funding and linkages This structured research program is related to GRDC funded (UA 00119), Assessing management options for enhanced soil phosphorus availability using rotations (January 2011 - June 2014) led by Senior Lecturer Dr. Ann McNeill. GRS10027: Grains Industry Research Scholarship - Heidi Waddell (UWA) Phosphorus-use efficiency of Austrodanthonia Contract Start 31/01/2011 Contract End Contact Dr. Alan Richardson Organisation Phone 30/01/2014 Project Code GRS10027 University of Western Australia 0262465189 Email [email protected] Project Summary Phosphorus is essential for plant growth. Many Australian soils have a low plant-available P content and agriculture relies on P-fertilisers for high productivity. However, P is a nonrenewable resource and global reserves are being depleted. Because low-P soils often have high P-sorption capacity, the P-balance efficiency of agriculture is low. On average 4 units of P are needed to produce only one unit of P in products. Hence, there is a need to improve the efficiency of P-use in agriculture. Species of the native perennial grass genus: Austrodanthonia (wallaby grass), are Pefficient. They have low external P requirements for growth and low critical internal P concentrations and yield well under low P stress. This suggests that Austrodanthonia species possess morphological and/or physiological adaptations that may help improve the P-use efficiency of agricultural systems. Further studies are required to determine the adaptations of this genus to low-P stress. I will examine 1. internal and external P-use efficiency of several Austrodanthonia species, 2. physiological and morphological mechanisms which confer P-use efficiency, and 3. implications of low-P conditions on the competitive ability of Austrodanthonia. The research will assist the use of Austrodanthonia in agriculture to improve the efficiency with which P-fertiliser is used. 6. Building Skills & Capacity GRDC National Region 49 GRS10028: Grains Industry Research Scholarship - Hollie Webster (UMU) Genetic factors and genes underpinning drought response in wheat GRDC National Region Contract 14/12/2010 Start Contract End Contact Prof. Rudi Appels Organisation Phone 13/12/2013 Project Code GRS10028 Murdoch University 08 9360 6229 Email [email protected] Project Summary 6. Building Skills & Capacity Project Aim 50 The objective of this project is to demonstrate the capacity of the sugar transporter gene IVR1 to confer drought resistance in wheat cultivars. Hence, the aim of this study is to further investigate how the expression of the sugar transporter gene IVR1 in wheat is affected in both water deficit and non-limited water environments. The experimental hypothesis is that the expression of the IVR1 gene is critical to sugar metabolism at heading, where it drives carbohydrate mobilization variation that provides the energy substrate (fructan) to developing pollen at meiosis. Project Overview This PhD project was formulated with the view to further investigating findings from recent studies carried out by Dr Jingjuan Zhang at Murdoch University and Dr Rudy Dolferus at CSIRO (Canberra). These studies identified key genes in wheat that are involved in plant sugar metabolism at heading (Ji et al 2009, Oliver et al 2007). In semi dwarf spring wheat ‘heading’ has been identified as the second most drought and frost stress sensitive growth phase (second only to seed germination), the effect of which manifests as sterile pollen development (Koonjul et al. 2005). Sterile pollen development, attributed to the down regulation of the sugar transporter gene IVR1, is directly correlated to yield loss by way of reduction in both grain number and grain size (Ji et al. 2005). Given that drought and frost stress at head development in wheat is a significant yield constraint throughout most Australian cropping regions it is expected that the project will deliver important research outcomes for the benefit of a wide range of grains industry stakeholders. At both molecular and phenotype characterization levels this PhD project is focusing on a unique set of 230 doubled haploid (DH) lines from a cross between the wheat varieties Westonia and Kauz (made available to the research project by Intergrain). Both Westonia and Kauz are considered to be drought tolerant but it is clear from the studies by Zhang (2008; PhD thesis Murdoch University) that they achieve this in different ways. It is expected that the analysis of a range of variables (discussed below) in the genetically structured population of 230 DH lines will provide new insights into genetic factors (and hence genes) determining drought tolerance. Key research areas will include: locating genomic DNA segments containing IVR1 on chromosome 3B using the WxK genetic map characterising the IVR1 gene family using BAC clones from Chinese Springs wheat gene expression studies of IVR1 under normal and water deficit conditions examining carbohydrate profile changes and fructan accumulation in reproductive tissue under water stress whole plant phenotype characterization of WxK for quantitative trait loci analysis See Appendix 2 for References 51 6. Building Skills & Capacity GRS10029: Grains Industry Research Scholarship - Karl Andersson (UNE) Manipulation of P sorption in agricultural soils GRDC National Region Contract Start 30/06/2011 Contract End Contact Dr. Christopher Guppy Organisation Phone 08/10/2014 Project Code GRS10029 University Of New England 02 6773 3567 Email [email protected] Project Summary This study will investigate the factors that influence phosphorus (P) sorption in selected soils in southern NSW; potential methods of P sorption management; and the physical, chemical and biological interactions affecting recycling of sorbed P. 6. Building Skills & Capacity P sorption in soils is a major limitation on the availability of the nutrient to plants. The P sorption curve of a soil describes the concentration of P in solution for a given concentration of total P. High sorption lowers the amount of available P for a given total P. 52 High P Sorption decreases the efficiency of fertilisers and recycling of organic and inorganic P. The mechanisms of sorption are influenced by: the amount of cations in solution (influenced by ECEC, texture and clay mineralogy); organic carbon and its fractions; pH and its effect on the levels of aluminium, iron and manganese in solution; liming history and the amount of calciim and the flocculation of aluminium hydroxides; and the concentration of aluminium and iron hydroxides. The phosphorus buffering index (PBI) provides a single point index on the degree of sorption and is routinely included in soil tests. Analysis of a database of over 8000 soil tests throughout southern NSW suggests that different soil properties may be dominant determinants of PBI under different management practices, soil types and locations. This study will test the hypotheses that: 1. PBI is driven by different soil factors under certain land use and/or geographic location; 2. PBI can be favourably manipulated by altering soil properties with amendments and/or farming practices. 3. P availability can be favourably managed by promoting P solubilising soil organisms. GRS10031: Grains Industry Research Scholarship - Laura Blake (UA) Use of novel wheat (waxy durum) in baking applications Contract Start 01/03/2011 Contract End Contact Dr. Andrew Barber Organisation Phone 01/12/2013 Project Code GRS10031 University of Adelaide 83482488 Email [email protected] Project Summary Use of novel wheat (waxy durum) in baking applications. Potential for reducing amount of vegetable shortening/butter required for baking through the use of this new wheat variety. Research into quality of food product, residual levels of oligosaccharides during production and health impacts and impact of different pretreatments. 6. Building Skills & Capacity GRDC National Region 53 GRS10034: Grains Industry Research Scholarship - Luke Holtham (UA) Identification of the Controller of Nitrate Transport in Maize GRDC National Region Contract Start 28/02/2011 Contract End Contact Dr. Trevor Garnett Organisation Phone 27/02/2014 Project Code GRS10034 University of Adelaide 08 8303 0179 Email [email protected] Project Summary Cereal crops take up only approximately 40% of the applied nitrogen fertiliser. Much of the other 60% leads to considerable environmental pollution. Since fertiliser is one of the largest expenses to producers, an improvement of nitrogen use efficiency in cereal crops is an important goal for lowering costs for farmers and reducing impact on the environment. Nitrate is the primary form of nitrogen taken up by cereal crops. The majority of nitrate is taken up by plants via NRT transporters. 6. Building Skills & Capacity Previous work in the NUE lab at the ACPFG has shown that the amount of nitrate taken up by maize varies greatly across the 54 lifecycle. This implies there is a control mechanism responsible for this variation in uptake. This project will identify the controller through transcript analysis of maize across the lifecycle. Candidate genes will be compared with candidate genes from a screen of Arabidopsis N transport mutants which will identify important genes for control of NRT transporters. The expression level of these putative controller genes will be altered in Arabidopsis and maize to examine the changes in nitrate uptake. GRS10035: Grains Industry Research Scholarship - Madeline Tucker (CUR) Minimising fungicide resistance 55 Contract Start 01/02/2011 Contract End Contact Prof. Richard Oliver Organisation Phone 31/01/2014 Project Code GRS10035 Curtin University of Technology 08 9266 4416 Email [email protected] Project Summary The project can be divided into two streams both concerning fungicide application in WA. The first element of my research will examine whether the risk of fungicide resistance increases or decreases with dose. Presently there are substantial gaps in knowledge about the effects of dose on the development of fungicide resistance. I will undertake research modelling the optimal dose and frequency of application to provide control of the pathogen whilst minimising the selection for resistant powdery mildew and tan spot (yellow spot) isolates. The second element of my research will examine known mutations conferring triazole fungicide resistance. Triazole fungicides have a single biochemical target, the sterol 14?demethylase protein (Cyp51), an essential enzyme in the biosynthetic pathway of ergosterol. The site specific mode of action and intensive use of these fungicides has led to the development of resistance in many organisms of agricultural importance. Recently, several mutational base changes in Cyp51 have been confirmed in triazole insensitive fungal pathogens of crops. Resistance to particular triazole fungicides has been shown to be associated with particular mutations although the causal relationships have not yet been established. The goal of this research is to create a series of isogenic isolates of Stagonospora Nodorum differing only in the sequence of Cyp51. The resistance spectrum of the isolates in response to different triazole fungicides will then be determined. 6. Building Skills & Capacity GRDC National Region GRS10036: Grains Industry Research Scholarship - Maree Horne (USQ) Interrelationships between Bipolaris sorokiniana isolates involved in spot blotch, common root rot and black point in winter cereals GRDC Region National Contract 28/02/2011 Start Contract End Contact Prof. Mark Sutherland Organisation Phone 27/02/2014 Project Code GRS10036 University of Southern Queensland 07 4631 2360 Email [email protected] 6. Building Skills & Capacity Project Summary 56 The fungal pathogen Bipolaris sorokiniana is a global pathogen of cereal crops which causes foliar spot blotch in wheat and barley and common root rot in various cereals. Spot blotch of barley and common root rot of wheat and barley are significant cereal pathogens in Australia. In addition some recent DEEDI data suggest that B. sorokiniana infections may lead to black point symptoms on barley grain. isolates from one type of diseased tissue (e.g. roots) to cause symptoms on other tissues (e.g. leaves & grain) will be examined across a standard set of wheat and barley lines, as will the potential of isolates from one host to cause disease on the other. Preliminary studies have suggested both host and tissue specificity exist among B. sorokiniana isolates (Knight et al. APP 2010). This project will investigate the genetic relatedness of B. sorokiniana isolates from grain, leaves and roots of wheat and barley. Initial studies will employ AFLP markers, while subsequent work will draw on information emerging from a USA-based project currently sequencing this pathogen. The ability of I will also conduct quantitative PCR studies on the extent of B.sorokiniana infection in barley grain to examine the relationship between fungal infection and blackpoint symptoms on mature grain. GRDC Region National Contract 14/02/2011 Start Contract End Contact Prof. Stephen Powles Organisation Phone 13/02/2014 Project Code GRS10037 University of Western Australia 08 6488 7833 Email [email protected] Project Summary Supervisors: Dr Michael Walsh (UWA, AHRI), Dr Ken Flower (UWA, WANTFA), Prof Stephen Powles (co-ordinating supervisor) (UWA, AHRI). The introduction of glyphosate tolerant canola into Australian agriculture offers superior weed control with the benefits of maintenance of notillage cropping systems. Glyphosate tolerant canola enables the use of glyphosate for broad spectrum weed control in the rotation (Walsh et al 2006) while enabling very early seeding and maintenance of no-tillage. These are all advantages in comparison with triazine tolerant canola. The glyphosate herbicide has low environmental risk and provides options for early seeding so as to maximize the length of the growing season. The weed wild radish (Raphanus raphanistrum L) is an economically damaging weed of global cropping. Wild radish adversely impacts large areas of Western Australian cropping and is a significant crop weed nationally. Herbicides are the major tool for wild radish control. However, in Western Australia this genetically variable weed species has shown the ability to evolve resistance to herbicides used for its control (Walsh et al 2001, 2007). Wild radish exhibits multiple herbicide resistance to group B (ALS inhibitors), C (PS2 inhibitors), F (PDS inhibitors)and I (Disruptors of plant cell growth) (Blackshaw 2001, Hashem etal 2006). Wild radish is a major cost to Australian cropping (estimated as $210 million in 2002, Madafiglio, 2002) . Wild radish is the number one dicot weed of WA cropping and ranked the third most difficult to control weed in Australia (Madafiglio, 2002). With the commercialization of glyphosate tolerant canola in Australian agriculture the herbicide glyphosate will be used to control wild radish within canola crops. Glyphosate when used at the label rate will control wild radish. Given there has been much past usage of glyphosate for wild radish control and the added selection pressure that can occur with glyphosate tolerant canola there is the risk of glyphosate resistance evolution in wild radish. Additionally, as both wild radish and canola are Brassica species there is the remote possibility that there could be hybridization between these species, enabling transfer of glyphosate resistance from canola to wild radish. This PhD aims to examine this potential risk for the evolution of glyphosate resistance in wild radish and mitigation strategies that can be employed to minimize the possibility of glyphosate resistance evolution in this species. The research will include widespread surveying of wild radish in glyphosate tolerant canola and agronomic strategies (crop competition, rotation, harvest weed seed management etc) which can help to minimize evolution risks. The research will 57 6. Building Skills & Capacity GRS10037: Grains Industry Research Scholarship - Michael Ashworth (UWA) Evolved Glyphosate resistance in wild radish (Raphanus raphanistrum L) populations with the use of Glyphosate resistant genetically modified canola build upon the body of knowledge within AHRI, DAFWA and elsewhere on wild radish biology and its management. The AHRI wild radish RIM model will be upgraded and used to simulate the efficacy of various management techniques and there will be glasshouse and agronomic field experiments to test management practices. The overall objective is research outcomes which help to preserve the sustainability of glyphosate tolerant canola and glyphosate in Australian cropping systems. Blackshaw, R.E (2001), Influence of Wild Radish on yield and Quality of Canola, Weed Science, vol 50, No 3, pp 344-349 6. Building Skills & Capacity Hashem, A, Pathan, S and French, B (2006), Wild Radish-Lupin competition: difference in competitive ability of lupin cultivars, Proceedings of fifteenth Australian Weeds conference, Weed Management society of South Australia, pp 391-394 58 Madafiglio, GP 2002, Population management of Raphanus raphanistrum L. (Wild Radish) by regulating seed production, PhD thesis, University of Western Sydney Powles, S, Preston, C (2006) Evolved Glyphosate resistance in Plants: Biochemical and genetic basis of resistance, Weed Technology, Vol 20, pp 282-289 Walsh, M.J., Owen, M.J., Powles, S.B. (2007) Frequency and distribution of herbicide resistance in Raphanus raphanistrum populations randomly collected across the Western Australian wheatbelt. Weed Research, 47, 542-550. Walsh, M.J., Duane, R.D. and Powles, S.B. (2001) High frequency of chlorsulfuron resistant wild radish (Raphanus raphanistrum L.) populations across the Western Australian wheatbelt. Weed Technology. 15, 199-203.. GRDC National Region Contract 31/03/2011 Start Contract End Contact Assoc Prof. Michael Kertesz Organisation Phone 30/03/2014 Project Code GRS10038 University of Sydney 02 8627 1022 Email [email protected] Project Summary The project will investigate interactions between selected high yielding, genetically diverse wheat genotypes and N-fixing associative plant-growth promoting bacteria in the rhizosphere (Azospirillum brasiliense and Herbaspirillum sp.). Inoculation with those bacteria is promising, thus previous studies have shown that N2-fixation can be obtained without or in addition to mineral fertilization. The effects of rhizosphere colonisation by these bacteria on root morphology and physiology and consequently on plant growth and grain quality will be monitored. The influence of crop cultivar and inoculant regime on the activity and identity of the natural soil microorganisms that catalyse N, P and S cycling in agricultural soils will be investigated using cutting edge molecular tools. Microbial community analysis will be used to study the dynamics of the overall rhizosphere community, in order to identify dominant organisms with impacts on plantgrowth promotion and disease suppression. In order to test effects of additional mineral nitrogen fertilization, varying levels of urea-N will be tested for their effects on N2 fixation, grain yield and quality, and rhizobacterial diversity. Results obtained in greenhouse experiments with three different soil types will be extended to short-term and long-term field trials near Narrabri, examining wheat growth both in monoculture and in rotation with legumes (chickpea and field pea). 59 6. Building Skills & Capacity GRS10038: Grains Industry Research Scholarship - Mirja Guldner (US) Enhancing plant nutrition with rhizosphere microorganisms GRS10039: Grains Industry Research Scholarship - Monica Kehoe (UWA) Unraveling the cause of black pod disease of narrowleafed lupin and developing a control solution GRDC National Region Contract 28/02/2011 Start Contract End Contact Prof. Roger Jones Organisation Phone 27/02/2014 Project Code GRS10039 University of Western Australia 08 9368 3269 Email [email protected] Project Summary Black pod disease of narrow-leafed lupin is causing devastating losses in lupin crops in south-west Australia. Possible causes include competition between pods on the main stem, nutrient deficiencies and infection by Bean yellow mosaic virus (BYMV) necrotic strain. 6. Building Skills & Capacity The impacts of environmental factors and cultivar on expression of the disease further complicate the issue. This research will be designed to establish whether BYMV alone is causing black pod disease, but only under certain climatic scenarios, or whether it has multiple causes. Achieving an effective control of the problem depends on understanding how 60 it is caused. A combination of field and glasshouse experiments combined with the use of immunological and molecular techniques will be used to answer these questions. It will benefit both WA and Australia as a whole, and regional and rural communities by establishing whether factors other than spread of BYMV by aphids play a significant role in causing black pod disease of narrow-leafed lupin, identifying the biological and climatic factors responsible for development of the disease and providing recommendations for control. GRDC National Region Contract 07/03/2011 Start Contract End Contact Dr. Peter Anderson Organisation Phone 06/03/2014 Project Code GRS10040 Flinders University 08 8201 5269 Email [email protected] Project Summary The aim of this research project is to investigate the role of sucrose transporters in increasing Barley yield and iron/zinc content of Barley grains. Recent research on wheat has found that increasing sucrose uptake in the grain stimulates a range of other effects, most notably an increase in grain protein content and an increase in yield (Weichert et al., 2009). When samples of these wheat grains were obtained and analysed, they also showed high levels of iron and zinc, up to a one third increase compared to the usual iron/zinc content of wheat (J. Stangoulis, pers. comm.). In addition to yield increase and biofortification of grain, such nutrient rich seeds could have the added advantages of higher vigour and viability particularly in micronutrient deficient soils (Welch and Graham, 2004). This project proposes to replicate these results in Barley grains, by constructing a transgene that will overexpress the Hordeum vulgare sucrose transporter under the control of an endosperm specific promoter. Sucrose dependent uploading of iron/zinc and the effect of increased sucrose uptake on yield and the amino acid profile of Barley grains will be examined to understand the complex mechanisms behind the metabolic regulation of these processes. Understanding of these fundamental processes in Barley will contribute more tools to use in breeding for grain crops with higher yield and nutrition content. 61 6. Building Skills & Capacity GRS10040: Grains Industry Research Scholarship - My-my Huynh (UF) Examining the role of sucrose transporter HvSUT1 in increasing yield and iron/zinc content in Rice GRS10041: Grains Industry Research Scholarship - Peter Gous (UQ) Understanding abiotic stress impacts on cereal starch structure and value-added quality through genetic and environmental screening GRDC Region National Contract 04/01/2011 Start Contract End Contact Prof. Robert Gilbert Organisation Phone 03/01/2014 Project Code GRS10041 University Of Queensland 07 3365 4809 Email [email protected] 6. Building Skills & Capacity Project Summary 62 My research proposal draws on a range of disciplines. The study aims to determine the effect abiotic tolerance genes have on commercially important traits such as starch properties and structural characteristics. Does the tolerance gene cause structural conformations and changes within these molecules (especially starch), such as are the molecular weight, branching structure and molecular size altered? Additionally how do these changes (if any) affect the cereal quality, as required by the value-adding sectors? Is it possible to sequence the genes that may cause these changes and are there gene interactions to those that incur tolerance? A key to this are new experimental and simulation tools developed at the University of Queensland enabling starch structure to be related to the underlying genetics and to grain properties. The research would ensure that abiotictolerant cereal?s grain properties remain desirable for industry and the grain remains at the required level of quality for the processing industries. This would give industry assurances of the grain quality from grain sourced from stressed environments, while grain growers would also be ensured a yield during variable climatic conditions. GRDC National Region Contract 01/03/2011 Start Contract End Contact Dr. Roger Mandel Organisation Phone 28/02/2014 Project Code GRS10042 Curtin University of Technology 08 9690 1526 Email [email protected] Project Summary Data aquisition for precision agriculture (PA) uses a range of information-rich methodologies and technologies to enable managers to better match agronomy and land use to spatial variability. However, a recent survey of WA farmers indicated data analysis and complexity was one of the greatest impediments to the adoption of PA (Mandel et al, 2010). Collecting the raw data is a comparatively simple task - selecting the correct tools and methods for data analysis is a challenge, particularly when applied to mixed farming systems. This project will apply precision agriculture tools across mixed farming systems, enabling the industry to achieve a higher return from the application of existing techniques and methodologies and improve business sustainability through precise and timely management decisions. The project will look at the following issues within the context of mixed farming enterprises: Which data layers are important in enabling farmers to delineate and effectively manage their cropping programmes and improve the production efficiency of their pasture based enterprises? The best approaches for using PA technologies to optimise landscape management from a whole-farm perspective, integrating information about crops, pastures, grazing systems and livestock productivity, especially in areas of low potential and in marginal country. Economic analysis will be undertaken to differentiate the potential impacts of PA data layers on farm economics, using models developed by UNSW and CSIRO for use in precision management. Theoretical outcomes will be compared with case studies on mixed farming businesses across Australia to refine these decision support models, to enable producers to better choose appropriate data layers for their particular situation. 63 6. Building Skills & Capacity GRS10042: Grains Industry Research Scholarship - Peter McEntee (CUR) The integration and validation of precision management tools for mixed farming systems. GRS10044: Grains Industry Research Scholarship - Rhiannon Schilling (UA) Evaluating the salt tolerance of transgenic wheat and barley GRDC National Region Contract 21/02/2011 Start Contract End Contact Prof. Mark Tester Organisation Phone 17/03/2014 Project Code GRS10044 University of Adelaide 06 8303 7159 Email [email protected] Project Summary 6. Building Skills & Capacity Salinity is major soil constraint influencing cereal crop production in Australia. By 2050, it is expected that 14 million hectares of agricultural land in Australia will be affected by dryland salinity alone. It is imperative that we develop high-yielding salt tolerant crops to enable profitable grain production in saline soils. A number of candidate genes which improve the salinity tolerance of plant species have been identified and now need to be transfered into commerically relevant wheat and barley varieties. 64 The aim of this project will be to test and evaluate the salt tolerance of transgenic wheat and barley lines expressing salinity tolerance genes, in both glasshouse and field conditions. This will involve the phenotypic and genotypic characterisation of each line in control and salt treatments. A high throughput imaging system, which allows non-destructive measurements of plant growth, will be used to assess relevant plant traits, such as shoot biomass, leaf health and plant water use. Particular emphasis will be placed on the ability of these transgenic lines to produce grain yield under saline conditions. Ultimately, this project offers the opportunity to identify transgenic wheat and barley lines with improved salt tolerance for future delivery to Australian grain producers. GRDC Region National Contract 01/11/2010 Start Contract End Contact Dr. Scott Chapman Organisation Phone 31/10/2013 Project Code GRS10045 CSIRO Plant Industry 07 3214 2254 Email [email protected] Project Summary Selecting for variation in flowering time in wheat has traditionally focused on variation in photoperiod and vernalisation responses. Higher temperatures under global warming have the potential to reduce the effectiveness of vernalisation as a means to control time to flowering. Planting dates may also need to change substantially such that photoperiod response may also become less desirable as a means to alter time to flowering. Alternative physiological mechanisms controlling time to flowering in wheat under high temperatures will be explored along with an assessment of variability in these traits in order to select for variation in time to flowering under high temperatures. These mechanisms have been termed 'earliness per se' but the underlying processes and genetic variability are yet to be determined. Wheat models that predict flowering time currently have very poor accuracy under high temperature conditions. The temperature responsiveness of different physiological processes that control time to flowering under high temperatures are poorly understood. This project will study high temperature responses of different processes that control time to flowering in order to improve the prediction accuracy of time to flowering in wheat under high temperatures. Genetic variability of temperature responsiveness will also be assessed in order improve prediction accuracy for a broad range of varieties. 65 6. Building Skills & Capacity GRS10045: Grains Industry Research Scholarship - Ryan Kearns (UQ) Genetic variability and physiological mechanisms controlling time to flowering in wheat under high temperatures GRS10061: Grains Industry Research Scholarship - Robert Syme (CUR) Comparative genomics of necrotrophic fungal pathogens GRDC National Region Contract Start 01/01/2011 Contract End Contact Prof. Richard Oliver Organisation Phone 31/12/2013 Project Code GRS10061 Curtin University of Technology 08 9266 4416 Email [email protected] Project Summary The project can be divided into two overlapping steams, a vertical project of Stagonospora nodorum and a horizontal project to optimise genomics strategies to help guide fungal genomics investigations. The first objective is the creation, curation and investigation of a Stagonospora nodorum database. The database will bring together diverse datasets including phenotypic, metabolomic, proteomic and genomic results from the ACNFP and collaborators. A centralised repository will serve as a deep, canonical reference for all the science related to S. nodorum, and also as a tool to facilitate bioinformatic queries and analysis. 6. Building Skills & Capacity The PhD project will also include investigation 66 of optimal sequencing strategies and bioinformatic protocols for small eukaryotic genomes (<;100MB), with particular regard to the Pleosporales. Reductions in the cost of short-read sequencing have enabled many small groups to fund small, isolated genome projects. It is unclear at the moment what combination of sequencing chemistries, assembly strategies and data curation protocols enable maximal scientific output. The knowledge and experience gained over the course of my project will be made available to GRDC partners and collaborators to help guide future genomic investigations of the Pleosporales. GRDC National Region Contract 28/02/2011 Start Contract End Contact Assoc Prof. Darryl Small Organisation Phone 27/02/2014 Project Code GRS10063 Royal Melbourne Institute of Technology 02 9925 2124 Email [email protected] Project Summary Bread is a major global stable although there are many variations in formulation, processing and styles of products. The high levels of starch found in most flours contribute to the quality of bake product (Sanz-Penella, Wronkowska, Soral-Smietana, Collar, & Haros, 2010) by a number of mechanisms, particular during fermentation as well as baking. These are increasing concerns (Fardet, Leenhardt, Lioger, Scalbert, & Remesy, 2006; Germaine et al., 2008; McKevith, 2004; Ring, Kramer, Schafer, & Behrendt, 2001; van Bakel et al,. 2009) regarding 1. Allergies and sensitivities of individuals to component in baked foods 2. The high digestibility of the products expressed in terms of glycaemic index (GI) The aims of this project are to revaluate these issues by focussing upon the components available for use in baking and to assess the significance of molecular interactions during bread making (Abdel-Aal & Rabalski, 2008; Grasten et al., 2007; Heinio, 2003; Leinonen, Liukkonen, Poutanen, Uusitupa, & Mykkanen, 1999; Pizzuti et al., 2006; Zaharieva, Ayana, Hakimi, Misra, & Monneveux, 2010). This will involve a series of phases emphasizing 1. A comparative study of flours from different cereal grain sources including various wheats and rye. This will build upon the limited evidence of differences between these in terms of digestibility and caused agents for sensitivities. 2. An evaluation of strategies which will minimise the breakdown of starches during digestion. Various hydrocolloid components will be utilised in these trials for their effectiveness delaying the release of simple sugars to the bloodstream. 3. A further plan will investigate the potential for reduce effects of baked products on sensitive consumers. This will be assess through the analysis of level of key protein compounds along with the impact of specific enzymes that might break these down during the bread making process The aims of this project are to revaluate these issues by focussing upon the components available for use in baking and to assess the significance of molecular interactions during bread making. This will involve a series of phases emphasizing A comparative study of flours from different cereal grain sources including various wheats and rye. This will build upon the limited 67 6. Building Skills & Capacity GRS10063: Grains Industry Research Scholarship - Oliver Buddrick (RMIT) Bread making procedure, product digestibility and impact on sensibility to gluten proteins GRS10248: Grains Industry Research Scholarship - Amanda Huen (US) Regulation and long-distance movement of nutrient starvation-responsive plant microRNAs GRDC National Region Contract 28/02/2012 Start Contract End Contact Prof. Peter Waterhouse Organisation Phone 27/02/2015 Project Code GRS10248 University of Sydney 02 9114 1322 Email [email protected] 6. Building Skills & Capacity Project Summary 68 Plants genetically coordinate their responses to biotic and abiotic stresses such as drought, pathogens and nutrient starvation. MicroRNAs are small gene-silencing RNAs which play an important role in the genetic regulation of plant development and stress responses. Certain microRNAs may act as long-distance signals for early communication of nutrient starvation between plant organs. However, the mechanisms governing these processes are currently unknown. I aim to characterise microRNA expression, mobility and function, with a focus on nutrient starvation-responsive microRNAs in the model plants Arabidopsis and Nicotiana benthamiana. I will be using techniques such as molecular cloning, mutation analysis and gene expression studies to determine factors which control mobile microRNAs at different stages of their biosynthesis and function. I will use a recently-developed reporter system for in-vivo tracking of microRNAs, their precursors, and target genes to observe the spatial and temporal patterns of microRNA biosynthesis, movement and gene silencing. This will give insight into the selective mechanisms of microRNA movement, and the biological relevance of microRNAs for nutrient responses at different stages of plant development. Greater understanding of microRNA-mediated regulation of nutrient homeostasis can be applied to plant breeding and modification programs to improve plant uptake and management of limited nutrient resources. GRDC Region National Contract 15/04/2012 Start Contract End Contact Dr. Donald Gardiner Organisation Phone 14/04/2015 Project Code GRS10249 University Of Queensland 07 3214 2370 Email [email protected] Project Summary Crown rot disease of wheat and barley is a chronic problem in the Australian industry (~AU$100 Million/year) and there are currently no known immune cultivars of either crop. In the absence of resistance in the host a better understanding of the pathogen (F. pseudograminearum) is required to enable improved crop protection strategies, in the medium term. Plant pathogen interactions are a complex interplay between a battery of host defence responses and mechanisms employed by the fungal pathogen to overcome these plant defences. In plant-pathogen interactions the pathogens employ a variety of mechanisms such as toxin production, enzymes to degrade plant defence compounds, production of effectors to perturb host signalling and enzyme to gain access to host nutrients. However, the specific mechanisms employed by F. pseudograminearum are largely unknown. The proposed co-host research group, CSIRO Plant Industry, has recently sequenced the genome of the responsible pathogen, F. pseudograminearum and uncovered unexpected large scale differences to the globally important wheat and barley Fusarium Head Blight pathogen, F. graminearum. This project will leverage this resource to identify and functionally dissect the molecular infection mechanisms that the fungal pathogen, F. pseudograminearum, employs to cause crown rot disease. 69 6. Building Skills & Capacity GRS10249: Grains Industry Research Scholarship - Andrew Kettle (UQ) Functional pathogen genomics and characterisation of the infection mechanisms of the wheat and barley crown rot pathogen, Fusarium pseudograminearum. GRS10258: Grains Industry Research Scholarship - Ella Brear (US) Charcterising potential symbiosome membrane proteins essential to the legume-rhizobium symbiosis GRDC National Region Contract 20/02/2012 Start Contract End Contact Dr. Penelope Smith Organisation Phone 19/02/2015 Project Code GRS10258 University of Sydney 6129067169 Email [email protected] Project Summary 6. Building Skills & Capacity Legumes are globally important as a high protein food source and for their capacity to enhance soil nitrogen. A contributing factor to their importance is their ability to develop symbiotic relationships with nitrogen fixing bacteria called rhizobia. Rhizobia provide the legume with fixed nitrogen in return for energy and nutrients required for bacterial metabolism. 70 Legumes house their symbiotic bacteria in nodules, where they are surrounded by a plant derived membrane called the symbiosome membrane. This membrane and the protein transporters embedded in it form an interface that regulates nutrient exchange. The initial stages of symbiosis development are well understood. However little is known about how the symbiosome membrane is formed and maintained throughout the symbiosis. Thus the aim of my project is to characterise proteins potentially involved in the development and maintenance of the symbiosome membrane in soybean (Glycine max). To do this I will measure expression of candidates throughout nodule development to determine when they are involved, I will attempt to localise the protein within the nodule by fluorescent protein tagging and immunolocalisation. The importance of the candidate to the symbiosis will be determined by silencing gene expression using artificial microRNA technology. GRDC Region National Contract 03/01/2012 Start Contract End Contact Assoc Prof. Rachel Burton Organisation Phone 02/01/2015 Project Code GRS10259 University of Adelaide 08 8303 1057 Email [email protected] Project Summary The proposed project will compose of three separate components: 1. Define the role of CslF6 antisense transcript in regulation of rice grain (1,3;1,4)-?-D-glucan production: Work during this project will continue from my honours results. 2. Identify key catalytic residues of CSLF6 by domain swapping and sitedirected mutagenesis: By interchanging small regions between enzymes that generate different (1,3;1,4)-?-D-glucan levels and structures, defined areas related to specific functions can be determined and potentially enhance or refine specific activities for commercial applications. 3. Identify potential regulatory factors of CSLF6 expression in cereal grain: How expression of the genes responsible for any aspect of (1,3;1,4)?-D-glucan biosynthesis is controlled remains unexplored. Isolating and characterising even one transcription factor has potential to help unravel many of the mysteries currently surrounding (1,3;1,4)-?-D-glucan biosynthesis and signalling in response to growth conditions, stresses and pathogen attack for instance. 71 6. Building Skills & Capacity GRS10259: Grains Industry Research Scholarship - George Dimitroff (UA) (1,3;1,4)-?D-glucan biosynthesis in the Poaceae: Exploring transcriptional regulation, associated expression and specific activities of biosynthetic enzymes. GRS10263: Grains Industry Research Scholarship - Jessey George (UA) Effect of small amounts of ammonium on the total nitrogen nutrition and on overall nutrition of maize GRDC Region National Contract 01/01/2012 Start Contract End Contact Prof. Mark Tester Organisation Phone 18/10/2013 Project Code GRS10263 University of Adelaide 06 8303 7159 Email [email protected] 6. Building Skills & Capacity Project Summary 72 Nitrate (NO3-) and ammonium (NH4+) are the predominant forms of nitrogen available to plants in agricultural soils. NO3-concentrations are generally 10 times that of NH4+ and this ratio is consistent in the pool of N available to crop plants. With soil solution concentrations of NH4+ being so much lower than NO3-, the contribution of NH4+ to the overall N budget of crop plants is often overlooked. Moreover, every year farmers apply huge amounts of nitrogenous fertlizer to improve the grain yield. Increasing the nitrogen use efficeincy of the help the farmers to reduce the cost cultivation for N fertilizers. This research will focus on the contribution of NH4+ in the nitrogen uptake of corn plants. The study will also investigate whether NH4+ has any effect on uptake and utilization of other nutrients and the apparent inhibition of NO3- uptake by NH4+. The gene expressions of various transporters that are involved in the N uptake will be measured to see the effect of NH4+ on N uptake and the activies of various enzymes that are involved in N assimilation will also be measured. Altering the expression levels of the genes that involved in increasing the NUE of maize will be used to develop cultivars which give high grain yield with less N. GRDC National Region Contract 01/01/2012 Start Contract End Contact Prof. Richard Bell Organisation Phone 20/03/2014 Project Code GRS10268 Murdoch University 08 93602370 Email [email protected] Project Summary Potassium (K) is required in high concentrations for plant growth and development, whereas, sodium (Na) is beneficial only at low concentrations, and becomes toxic at high concentrations. In saline and sodic soils, Na interferes with the transport and cytosolic functions of K and affects plant growth and development due to depression of K uptake by competing Na ions and high Na+/K+ ratio.However, as K and Na ions are similar in ionic radius and hydration energies, the function of K can be partially replaced by Na under K deficiency. The extent of substitution varies between plant species and even between cultivars of the same species. Potassium is a crucial element for crop production in saline and sodic soils and hence it is important to understand its interaction with Na especially on duplex soils and in droughtprone growing conditions. The main aims of the research are: to study the role of Na in K nutrition of wheat in saline/sodic soils and in drought-prone environments; to understand the potential replacement of K by Na in wheat genotypes differing in K use efficiency and salt tolerance; to evaluate the relationship between K responses and levels of sub-soil cations (especially Na) in duplex soils; to understand the molecular genetics underlying the Na replacement of K in wheat genotypes. 73 6. Building Skills & Capacity GRS10268: Grains Industry Research Scholarship - Karthika Krishnasamy (UMU) Wheat potassium nutrition in saline and/or sodic soils and in drought-prone environments GRS10275: Grains Industry Research Scholarship - Max Bergmann (UWA) The use of potassium to improve water usage, growth and yield of canola in European high-yielding and Australian low-yielding farming systems GRDC Region National Contract 01/01/2012 Start Contract End Contact Dr. Ken Flower Organisation Phone 29/09/2014 Project Code GRS10275 University of Western Australia 08 6488 4576 Email [email protected] 6. Building Skills & Capacity Project Summary 74 This study will determine if canola water use efficiency and drought tolerance can be improved through K nutrition. It will be linked to an existing GRDC funded project with the Department of Agriculture and Food in Western Australia (DAFWA) studying the impact of plant k status on water use efficiency, as part of the GRDC project 'Improving profit from fertiliser through knowledge-based tools that account for temporal and spatial soil nutrient supply'. This link is through Dr Craig Scanlan at DAFWA who will also be one of my PhD supervisors. The intended project will include detailed greenhouse and field studies to clarify the physiological response of canola to increased K levels and drought. These experiments will be complemented with detailed studies of rootsoil interaction intending to assess the relations between K levels of soils on root water uptake. The experiments will consist of combining measurements of root conductivity (Steudle et al. 1987) with imaging techniques as neutron radiography to observe roots and water distributions in soils (Carminati et al. 2010). In detail some of the experiments will be carried out in the very near zone of the rhizosphere and the soil to study the varying water flux due to the K level in the soil (Carmenati et al. 2007). In addition, larger-scale field studies in Western Australia and Germany will be done to evaluate the impact of K nutrition in different environments. The field trials will provide more relevant information for farmers on expected yield improvements and crop water use efficiency from additional K fertilisation. Moreover, the number of environments and climatic conditions evaluated will be expanded by using the APSIM (Agricultural Production Systems Simulator) biophysical model. It is intended that he information from this research will also be used to update the canola module of APSIM. GRDC National Region Contract 27/02/2012 Start Contract End Contact Assoc Prof. Darryl Small Organisation Phone 26/02/2015 Project Code GRS10290 Royal Melbourne Institute of Technology 02 9925 2124 Email [email protected] Project Summary In the context of global pressure upon environmental determinants of grain quality, recent research has explored the potential of lower protein wheats for production of instant Asian noodles. With rapid increases in demand for these products there is now a need to better understand the potential contribution of these to health and wellbeing. The purpose of this project is to investigate the forms, stability and availability of two significant components that are important to health and wellbeing. Dietary vitamin E and selenium are potentially contributors to antioxidant properties and these will be studied in grains, flours and related food products. The challenges of this research include the development of more sensitive analysis procedures so that even low levels of the components can be measured reliably. A further emphasis will be on techniques which allow reliable quantitation of the various individual forms of each of the two components so that the influence of environment, processing and storage can be evaluated. The likely outcomes are an understanding of factors influencing product quality while enhancing the storage stability, value, appeal and marketability of grains and their products. 75 6. Building Skills & Capacity GRS10290: Grains Industry Research Scholarship - Wenywati Tjong (RMIT) Forms, analysis and stability of vitamin E and selenium in grains and grain products GRS10329: Grains Industry Research Scholarship - Christina Delay (ANU) Investigating the role of root architecture regulators as mediators of environmental information in root development GRDC Region National Contract 13/02/2012 Start Contract End Contact Assoc Prof. Michael Djordjevic Organisation Phone 12/02/2015 Project Code GRS10329 Australian National University 02 6125 3088 Email [email protected] Project Summary 6. Building Skills & Capacity Root architecture and nutrient uptake strongly influence crop fitness and productivity. A major nutritional factor modulating root development and overall yield is nitrogen (N). Current crops are not optimized for efficient N-fertilizer use; commonly 50% of applied N is lost. Little is known about how N and other environmental parameters interact with innate root development pathways to optimise crop performance. 76 Our group recently discovered a novel family of peptide regulators called RARs (Root Architecture Regulators), which are unique to higher plants and root knot nematodes (RKNs). Our world-leading studies show that plant RARs respond to N and CO2, dictate how plants regulate their N-uptake machinery and control root architecture. RKN RARs are integral to nematode parasitism. The proposed project will elucidate: (i) the function of individual RARs in orchestrating root architecture and N use efficiency; (ii) how RARs are regulated by environmental cues (nitrogen, carbon, nutrients and water); and (iii) how they are implicated in RKN infection. The initial studies will use Arabidopsis. Our findings will be applied to crops such as wheat and canola (a close relative of Arabidopsis) and will contribute to more environmentally sustainable and efficient agricultural practices. GRDC National Region Contract 13/02/2012 Start Contract End Contact Prof. Mike McLaughlin Organisation Phone 12/02/2015 Project Code GRS10335 University of Adelaide 08 8303 8433 Email [email protected] Project Summary Foliar fertilisation is a common in-crop technique used to apply micronutrients (e.g. zinc, copper and manganese) and in some cases nitrogen. This practice is less common for phosphorus (P) but the ability to use P tactically is attractive due to the small proportion of soil P present in a bioavailable form and the strong affinity for added P to be fixed in soils. This results in a large inital P application rate to soils to enable sufficient P throughout the growing season. Despite this initial application of P, crops may lack the nutrients required later in the growing season to maximise their yields. The application of foliar nutrients to slightly deficient crops may help to give them the boost they need. This project will identify the key factors controlling wheat leaf uptake of nutrients, particularly phosphorus in combination with micronutrients. The knowledge of these properties will result in testing of formulations with higher penetration ability (for example adjuvants or solution pH suited to wheat leaves) and comparison with existing formulations. The formulations will be tested for their efficacy of plant uptake using isotopic tehcniques. The formulations will be tested at a range of wheat growth stages to identify the optimal timing. 77 6. Building Skills & Capacity GRS10335: Grains Industry Research Scholarship - Courtney Peirce (UA) Foliar Fertilisation Of Wheat Plants - Phosphorus in Combination with Other Nutrients GRS10336: Grains Industry Research Scholarship - Tawanda Kapfuchira (US) Genetics of Biofortified Wheat GRDC National Region Contract Start 01/01/2012 Contract End Contact Prof. Richard Trethowan Organisation Phone 24/02/2014 Project Code GRS10336 University of Sydney 02 9351 8800 Email [email protected] 6. Building Skills & Capacity Project Summary 78 The concentration and bioavailability of essential elements such as iron and zinc is very low in modern wheat cultivars. Over two billion people in the world suffer from iron and zinc deficiency, including in the developed world, breeding programs aimed at improving the nutritional quality of wheat are necessary. A population of wheat lines is available to study the genetics of biofortified wheat using molecular markers. The population was developed from a cross between parents contrasting for iron and zinc concentration, inulin (a bioavailability enhancer) and phytate (a bioavailability inhibitor). One parent is an Iranian landrace and the other is a mutant line of a US cultivar. The expression of the traits will be determined in contrasting environments over three years. Once materials with both optimal and suboptimal combinations of micronutrient bioavailabilities are identified, their impact on nutrition will be confirmed in animal feeding tests, using broiler chickens. The stability of the biofortification in wheat lines across environments and years will be determined. The relationship between yield, protein and mineral concentration and bioavailability will also be investigated. Field trials will be conducted at the Plant Breeding Institute in Cobbitty and the IA Watson Grains Research Centre in Narrabri. GRDC National Region Contract 01/01/2013 Start Contract End Contact Assoc Prof. Christian Nansen Organisation Phone 31/10/2015 Project Code GRS10404 University of Western Australia 08 6488 8672 Email [email protected] Project Summary This PhD project will provide new insight into aphid infestation distribution patterns in commercial canola, and will characterize the spread (epidemiology) of aphid infestations. This information will be used to develop feasible and reliable monitoring/detection tools and will provide farmers and consultants with better knowledge about when and where to look for emerging aphid infestations. That is, further insight will be provided into how fast aphid infestations spread, and how far apart and how many plants should be inspected in order to accurately estimate aphid densities in a given sampling space. Action thresholds will be developed for aphids in canola in which we consider direct effects of feeding to growing crops in Spring. With better and more reliable monitoring/detection tools and action thresholds for aphid infestations, we predict that it will be possible to reduce pesticide applications targeted at aphids by applying them more effectively (higher spray volume and droplet size) on a ‘when and where needed basis’. This project will also include reflectance-based detection of aphid infestations as part of a long-term research effort into development of automated monitoring/detection based on rover based technology and hyperspectral imaging. 79 6. Building Skills & Capacity GRS10404: Grains Industry Research Scholarship - Dustin Severtson (UWA) Detection and epidemiology of spring aphids in canola GRS10405: Grains Industry Research Scholarship - Guoquan Liu (UQ) Manipulating seed storage proteins to enhance sorghum digestibility GRDC National Region Contract 02/04/2013 Start Contract End Contact Prof. Ian Godwin Organisation Phone 01/04/2016 Project Code GRS10405 University Of Queensland 07 3346 9966 Email [email protected] Project Summary Low digestibility of sorghum grain is a major limitation to sorghum productivity and profitability. Sorghum grain is usually discounted as feed grain when compared to wheat and barley, predominantly because of its lower digestibility and energy conversion. 6. Building Skills & Capacity Sorghum endosperm has a particular structure: starch and protein matrix, whereby starch is often bound by the major storage proteins, the kafirins including alfa-, beta-, gamma- and delta- kafirin. However, beta-, gamma-kafirin and their disulphate bond have been reported to be related to sorghum low digestibility. 80 The Godwin lab has cloned and characterised genes of major sorghum seed storage proteins including beta-, gamma-kafirin and endosperm protein disulphate isomerise (PDI) gene. Recently, in this lab, sorghum transformation efficiency has been improved from 1% to 20.7%. As a result, the research has been published on the journal of Plant Cell Reports in 2012. The project now aims to manipulate sorghum starch and protein matrix at molecular level to improve grain digestibility for various end-uses (feed, food, and bio-materials). The major aims of this project are: To improve sorghum transformation efficiency; To manipulate beta-, gamma-kafirin genes involved in sorghum digestibility; To manipulate sorghum endosperm PDI gene involved in digestibility. GRDC National Region Contract 29/03/2013 Start Contract End Contact Dr. Suong Cu Organisation Phone 28/03/2016 Project Code GRS10407 University of Adelaide 08 8313 6738 Email [email protected] Project Summary A critical requirement during malting and the initial stages of brewing is hydrolysis of plant cell walls. Failure to acheive this results in a highly viscous solution that causes filtration and other processing problems during brewing. This quality defect was the reason the barley variety Hindmarsh failed malting accreditation and breweries are begining to adopt new filtration technologies that will increase process sensitivity to undegraded cell wall components so this issue is of increasing importance to breeders and the malting and brewing industry. The long chain polysaccharide beta-glucan is the major component of barley cell walls. Previous research by the University of Adelaide has identified a novel form of the enzyme beta-glucanase in wild barley which exhibits higher thermostability and activity levels than is seen in cultivated varieties. The project will identify the DNA and protein sequence changes responsible for these differences allowing development of diagnostic molecular markers and structure/function analysis based on the crystal structure of barley beta-glucanase. The wild barley derived form of the enzyme has been crossed into elite barley and its impact on malting and brewing quality will be evaluated to quantify potential industry benefit. 81 6. Building Skills & Capacity GRS10407: Grains Industry Research Scholarship - Juanita Lauer-Smith (UA) Characterisation of novel forms of betaglucanase in malting barley GRS10408: Grains Industry Research Scholarship - Julia Andrys (UMU) Spatial and Temporal Distribution of Severe Weather Events and their Impact on Grain Crops GRDC National Region Contract 01/01/2013 Start Contract End Contact Prof. Tom Lyons Organisation Phone 30/08/2015 Project Code GRS10408 Murdoch University 08 93602413 Email [email protected] Project Summary 6. Building Skills & Capacity Severe weather episodes such as frost, drought, high temperatures and high intensity rainfall can be devastating to crop yields. While the frequency of frost may decline in response to climate change, it is expected that the occurrence of extreme weather events will increase overall in the SWWA in the future. To develop the best risk management strategies for climate change, it is important therefore to understand not only when these events are likely to occur but how they will be distributed throughout the region. 82 Using the state of the art regional climate model WRF, and iVEC advanced computing resources, this project will examine 30 years of high resolution data of the region?s historical climate and a further 30 years under projected climate change to compare the current distriubution of extreme weather with the expected future distribution of severe events in the SWWA. The 10km scale of the model data output will allow this research to consider the highly localised weather patterns that are beyond the scope of lower resolution climate data. With an emphasis on farm management, this project will focus on the May-October growing season, in particular the spring months which are critical in the cropping calendar. GRDC Region National Contract 01/01/2013 Start Contract End Contact Dr. Rao (RCN) Rachaputi Organisation Phone 01/07/2015 Project Code GRS10421 University Of Queensland 07 41600737 Email [email protected] Project Summary The objective of this project will be to develop accurate molecular markers for the identification and selection of key agronomic and quality/marketing traits in peanut genotypes. Initial research of the relevant literature reveals that traditional Sanger DNA sequencing technologies, which are expensive and time consuming, have discovered relatively few functional molecular markers in cultivated peanuts. This project will employ next-generation sequencing technologies to discover new molecular markers, single nucleotide polymorphisms (SNPs) and diversity arrays (DArT), which will be validated using phenotypic analysis of selected populations, including a unique recombinant inbred line (RIL) population developed as part my honours project (which was funded under a GRDC Honours Scholarship). The key traits that will be considered for marker-assisted selection (MAS) include foliar disease resistance, crop maturity, pod constriction, kernel size/shape, shelling percent, kernel blanchability, kernel moisture dry down, oil content and fatty acid composition. The validated markers will ultimately be utilised in a MAS breeding program that will complement traditional breeding methods used in the PCA-GRDC-DAFFQ funded Australian Peanut Genetic Improvement Program (APGIP). MAS offers plant breeders the opportunity to achieve early generation selection of key traits in the breeding program, enabling a larger number of smaller yet desirable populations to be progressed in the breeding process. The early generation selection of key traits can save precious time and resources in large scale breeding programs, especially for traits under complex genetic control or when phenotypic traits are difficult and/or expensive to measure (Pl. Biotech. J, vol 8, p. 2). 83 6. Building Skills & Capacity GRS10421: Grains Industry Research Scholarship - Daniel O'Connor (UQ) Development of functional molecular markers for key agronomic traits in the culitvated peanut, using next-generation sequencing technologies GRS10432: Grains Industry Research Scholarship - Thomas Jason Major (ANU) Communicating controversial science to farmers GRDC National Region Contract 01/07/2013 Start Contract End Contact Dr. Rod Lambers Organisation Phone 01/07/2016 Project Code GRS10432 Australian National University 02 6125 0498 Email [email protected] Project Summary Precise question will be refined during the early stages of PhD. Broad concept is below Communicating controversial science to farmers How do a farmer’s values influence their perception/interpretation of controversial science such as climate change? 6. Building Skills & Capacity Do these values differ for different science/technologies such as GM crops and how does this influence the acceptance or otherwise of the science and how it might transfer to changes in farm management? 84 Where are farmers getting their scientific information relevant to their agronomic practice? Who are the influencers ? who do they trust as information sources and why? What are the factors driving this trust/distrust? How are their values/biases influencing their interpretation from these sources and how they implement or make use of the information to manage their farm? How are we framing relevant aspects of climate science (or other technologies) for various farmers? Have we framed it in a way that aligns with their values? Does this even matter? How are they interpreting this information and what are they doing with it? What, if any, are the barriers to the access, interpretation, of this science and its proposed implementation on the farm? GRS10437: Grains Industry Research Scholarship - Kasra Sabermenesh (UA) Responses of maize roots to nitrogen supply Contract Start 01/01/2013 Contract End Contact Dr. Trevor Garnett Organisation Phone 21/02/2014 Project Code GRS10437 Australian Centre for Plant Functional Genomics 08 8303 0179 Email [email protected] Project Summary Nitrogen fertiliser is one of the largest input costs for Australian cereal growers. Unfortunately, cereal crops take up only 40% of the nitrogen fertiliser applied, leading to economic losses and considerable environmental pollution. This low uptake efficiency is a major issue but it also provides a substantial opportunity as there is plenty of scope for improvement. The root system plays a fundamental role in nutrient uptake from the soil, but the responses of roots to changes in nitrogen supply are still relatively poorly understood, chiefly because roots are generally in soil and difficult to study. Previous work at the ACPFG has shown that maize lines that can grow well with low nitrogen have better root nitrogen uptake characteristics. I have identified when in the lifecycle maize lines are responding to reduced nitrogen supply and I am now characterising the processes underlying this important plant response. I will use a combination of classical plant physiology together with state of the art transcriptomics and metabolomics to carry out this investigation. Understanding how this response differs between lines with contrasting responses to low nitrogen will allow us to identity root traits important to improving NUE. 6. Building Skills & Capacity GRDC National Region 85 GRS10440: Grains Industry Research Scholarship - Kevin Adamson (Sunshine Coast) Investigation of metabolic regulatory genes and hormones in pest snails. GRDC National Region Contract 01/01/2013 Start Contract End Contact Dr. Scott Cummins Organisation Phone 06/02/2015 Project Code GRS10440 University of Sunshine Coast 07 5456 5501 Email [email protected] 6. Building Skills & Capacity Project Summary 86 Several species of snails have become major pests in agricultural regions of Australia, feeding on crops, clogging harvesting machinery and threatening exports by contaminating grain. For example, pest snails are costing grain growers on the Yorke Peninsular almost $60,000 per farm. These snails survive the hot Australian summers by entering a state of suspended animation known as aestivation. In aestivation, snails cease movement to conserve energy and seal themselves against water loss and reduce heart rate, breathing and metabolism up to 90%. Remarkably, snails can survive for months (even in temperatures exceeding 50?C!) until conditions improve, at which time they become reanimated within 5 minutes. These snails provide an excellent model to identify the basic principles of metabolic depression. Currently we have little understanding of the genes and hormones that control this in any animal. A comprehensive molecular study of the genetic control of metabolism, comparing active and aestivating snails, will clarify which genes and hormones trigger and regulate this inactive state. This knowledge will provide the genetic tools for a novel biological control strategy to be developed that would aim to prevent snails from entering aestivation, leading to high snail mortality. GRDC Region National Contract 01/01/2013 Start Contract End Contact Assoc Prof. Thomas Martin Organisation Phone 11/03/2015 Project Code GRS10460 University of Western Australia 08 6488 3331 Email [email protected] Project Summary Plant nitrilases are involved in auxin and ethylene biosynthesis. These major plant hormones regulate aspects of plant development such as leaf senescence. The involvement of nitrilases in leaf senescence, especially in the role of chlorophyll retention, has been recently identified using stay green mutants which maintain green-ness whilst progressing into senescence. Stay green mutants have been found in both maize and rice. The interaction between nitrilases and 14-3-3 proteins that I identified is a novel discovery and has not been thoroughly explored. As 143-3 proteins are major regulatory proteins in plant development, growth and metabolism, this interaction may be fundamental to the role of nitrilases and thus will provide new insight into the role and control of nitrilases in plants undergoing senescence. My PhD project is an expansion of this work to fully explore the mechanism and impact of nitrilase and 14-3-3 interactions using the model plant Arabidopsis thaliana. This research will provide the groundwork to understand how plant nitrilases are regulated and how they are involved in stay green mutants. Outcomes will be transferred to control nitrilases activity in cereal and oilseed crops to control their timing of senescence and alter their responses to biotic stresses such as pathogens. 87 6. Building Skills & Capacity GRS10460: Grains Industry Research Scholarship - Julia Man (UWA) Characterisation of Arabidopsis nitrilases and 14-3-3 interactions in the processes of photosynthetic loss, leaf senescence and plant stress GRS10471: Grains Industry Research Scholarship - Kylie Foster (USA) Whole-ofplant study of salinity tolerance: A mathematical modelling approach GRDC National Region Contract 01/01/2013 Start Contract End Contact Prof. Stan Miklavcic Organisation Phone 29/07/2015 Project Code GRS10471 University of South Australia 08 8302 3788 Email [email protected] 6. Building Skills & Capacity Project Summary 88 Abiotic stresses such as high salt levels in soils can severely reduce cereal crop plant productivity. Currently, high salinity affects two-thirds of Australian cereal crops. compartmentalization of ions in the shoot? None of the existing models of water transport in plants are currently suitable for analysing the transport of ions. To increase plant salinity tolerance it is necessary to manipulate the transport of ions (e.g., sodium and chloride) through a plant. However, this requires knowledge about how ion transport through a plant occurs. In particular, it is necessary to identify the key points in this transport pathway to target in order to generate a salt-tolerant cereal variety. For example, is targeting the initial influx of ions from the soil the best method for increasing plant salinity tolerance, or should more effort be directed towards increasing the The overall aim of this research project is to develop a time-dependent, whole-of-plant quantitative model of water, ion and other solute transport that would aid understanding of the biophysical mechanisms and processes responsible for increasing plant salinity tolerance. It is envisioned that the results will help guide plant geneticists in their search for specific genetic traits that enhance a plant's ability to tolerate salinity. GRDC National Region Contract 02/01/2013 Start Contract End Contact A/Prof. Dusan Losic Organisation Phone 26/02/2014 Project Code GRS10474 University of Adelaide 08 8313 5446 Email [email protected] Project Summary The Australian grains industry represents ~24% of the country’s total agricultural export, and is also essential in sustaining domestic human and animal food requirements. The industry is able to compete with international markets through its strict quality control protocols, which will not tolerate live insects in harvested grain. The use of traditional insecticides such as phosphine is becoming inadequate, due to pest insects developing resistance to current products used. Additionally, there is negative public perception surrounding the use of toxic chemicals and pesticides for pest management on stored grains. Both are critical issues which threaten to jeopardise the industry’s delivery of high quality products to target markets. In order to address these two obstacles, current research has been focussed on the use of chemical and resistance free diatomaceous earth (DE) as an alternative insecticide for stored grain. The specific aims of this project are to investigate the fundamental biological aspects of common grain pests, and the mechanisms which contribute to the successful use of DE to eradicate them. In particular, laboratory trials will be undertaken to assess the critical parameters required to increase the effectiveness of DE micro-particles which will enable the design of optimum DE formulations for grain storage. 89 6. Building Skills & Capacity GRS10474: Grains Industry Research Scholarship - John Hayles (UA) Nanotechnology and chemical-free approach for the protection of stored grain GRS10477: Grains Industry Research Scholarship - Volkan Arici (USQ) On-the-go proximal soil exchangeable ion sensing for precision management of sodicity GRDC National Region Contract 04/03/2013 Start Contract End Contact Dr. John Bennett Organisation Phone 03/03/2016 Project Code GRS10477 University of Southern Queensland 746311890 Email [email protected] Project Summary 6. Building Skills & Capacity Soil sodicity continues to limit crop yield potential, with 2005 estimates for lost economic potential at AUS$1035 million. However, while the potential to increase captial gain is real, the manner by which to identify sodic problem areas and apply amednments presents an economic risk that often offsets the attraction of the potential gains. Furthermore, methods to treat sodicity are usually applied in a paddock scale blanketrate approach, as precision agriculture (PA) tools do not exists for the determination of exchangeable cations, specifically sodium (Na). 90 With recent advances in proximal soil nutrient sensing using electrochemical sensors, soil ion kinetics have been used to provide rapid determination of total ion concentrations. There exists the potential to further analyse soil ion kinetic functions, based on sensor lagrate and short-term ionic spike, to seperate soluble and exchangeable ion signatures. Additionally, through the use of NIR and MIR spectroscopy soil moisture and cation exchange capacity can be proximally measured, respectively. Through combination, further development and refinement of these three proximal methods it is proposed that a proximal contour map for exchangeable sodium percentage (key sodicity indicator) can be produced. In doing so, it would be possible to identify and prioritise sodic portions of paddocks and apply amendments with precision. Thus, lost economic potential could be realised and risks offset. GRDC Region National Contract 01/01/2013 Start Contract End Contact Prof. Jim Prately Organisation Phone 12/08/2015 Project Code GRS10483 Charles Sturt University 02 69332862 Email [email protected] Project Summary Herbicide resistance is an ever increasing problem in cropping systems. Annual ryegrass is Australia’s most damaging cropping weed, and has developed resistance to multiple herbicides throughout Australia. A factor in effectively treating resistance is the accurate identification of resistant weeds. Currently, identification of the resistant status can take from 4 to 10 months. This waiting time could be dramatically reduced to as little as 3 weeks with the utilisation of Diversity Array Technologies (DArT). DArT allows the analysis of young plants, meaning that resistance could be determined within a growing season, allowing more effective resistance management techniques to be employed. To use DArT, two milestones must be met: the creation of a library of the plants DNA; and the identification of desired markers. Having already developed a library for annual ryegrass, this project aims to identify the markers responsible for herbicide resistance in group B and M herbicides. To achieve this, DNA will be extracted from known resistant annual ryegrass varieties and carefully bred progeny. This DNA will then be analysed and genetic markers responsible for resistance determined. 91 6. Building Skills & Capacity GRS10483: Grains Industry Research Scholarship - Aaron Preston (UCS) Determination of genetic markers for herbicide resistance in annual ryegrass using diversity array technology GRS10490: Grains Industry Research Scholarship - Jana Phan (UA) Identification of arabionxylan biosynthetic genes in plants GRDC National Region Contract Start 19/08/2013 Contract End Contact Assoc Prof. Rachel Burton Organisation Phone 18/08/2016 Project Code GRS10490 University of Adelaide 08 8303 1057 Email [email protected] 6. Building Skills & Capacity Project Summary 92 Wheat is Australia’s main cereal crop and is of great economic importance. It is also the predominant cereal consumed in most developed countries. The major dietary fibre from the grain cell walls is a polysaccharide called arabinoxylan. Consumption of soluble dietary fibres, such as arabinoxylan, is a key measure in preventing serious human diseases such as type 2 diabetes, cardiovascular disease and colon cancer. These polysaccharides also influence industrial uses of wheat such as baking, pasta and noodle manufacture. We currently understand little of how arabinoxylan is made by the plant, including the identity of the main biosynthetic genes. This information would be transformational, allowing the development of molecular markers for breeders to select for the most beneficial type of arabinoxylan and providing tools for biotechnological approaches to influence the amount and structure of grain fibre. Wheat grain contains a complex array of tissues and a large hexaploid genome, making gene discovery very complicated. To overcome this, we aim to use Plantago as a model system. Plantago seeds make a coating of mucilage very rich in arabinoxylan. This enriched system can be used to identify genes controlling arabinoxylan biosynthesis enabling the equivalent genes to be studied in wheat and other cereals. GRDC National Region Contract Start 01/07/2013 Contract End Contact Dr. Scott Chapman Organisation Phone 16/03/2015 Project Code GRS10501 University Of Queensland 07 3214 2254 Email [email protected] Project Summary As arable farmland is limited in Australia, grain production must be increased through development of wheat cultivars with higher yields. Abiotic stresses such as high temperature exposure can reduce grain yield significantly, yet this is currently an underresearched area and very important within the Australian grain industry, which is sensitive to reduction of yield during heat events and in response to changing climates. It is important to identify critical physiological traits associated with high yield and yield stability under heat stress and to understand the molecular and genomic factors involved, to identify cultivars with high yielding, temperature tolerant phenotypes. By identifying these critical traits to increase grain production under adverse environmental conditions, this research will contribute to future production security in the grains industry during heat events. Using field and climate controlled trials, combined with QTL analysis, the objectives of this project are; 1. Evaluate and phenotype existing genetic diversity for traits associated with heat tolerance using field and controlled environments 2. Utilize and develop novel physiological traits and tools to identify heat tolerant parental germplasm for use in breeding programs for higher grain production/yield stability. 3. Identify useful genetic information to enable more efficient selection for traits associated with high yield under heat stress for breeding applications. 93 6. Building Skills & Capacity GRS10501: Grains Industry Research Scholarship - Kimberly Alexander (UQ) Identification of quantitative trait loci associated with heat tolerance in wheat for increased fertility and grain development/genetic variability in heat responses for grain development GRS10505: Grains Industry Research Scholarship - Oliver Mead (ANU ) Investigating the role of gamma-aminobutryic acid in pathogenicity in fungal wheat diseases GRDC National Region Contract 01/08/2013 Start Contract End Contact A/Prof. Peter Solomon Organisation Phone 31/07/2016 Project Code GRS10505 Australian National University 02 6125 3952 Email [email protected] Project Summary Septoria nodorum blotch disease is the third most economically significant disease to the wheat industry in Australia, causing an average loss of over $100 million dollars each year. 6. Building Skills & Capacity In my honours project, previously invested in by the GRDC, this disease was used to determine the role of a metabolic pathway, previously unexamined in fungi. This pathway, called the ?-aminobutyric acid (GABA) shunt was found to be crucial to septoria nodorum blotch disease development. In the proposed project, I intend to determine the cause of this 94 requirement for pathogenicity, as well as determine whether this requirement is seen in other diseases significantly affecting the wheat industry. To do this, I shall investigate the role of this pathway in septoria nodorum blotch, tan spot and septoria tritici blotch diseases. This will involve use of a variety of genetic and chemical analysis techniques, firmly established in the Solomon lab, to further our understanding of this pathway during fungal infection of the wheat plant. GRDC National Region Contract 01/01/2013 Start Contract End Contact Dr. Jacqueline Batley Organisation Phone 01/04/2015 Project Code GRS10507 University Of Queensland 07 3346 9534 Email [email protected] Project Summary A thorough understanding of the relationships between plants and pathogens is essential if we are to continue to meet the agricultural needs of the world’s growing population. The aim of this project will be to add to the growing genomic knowledge of Brassicas, through the identification of candidate resistance genes in Brassica napus (canola) for its most devastating fungal pathogen, Leptosphaeria maculans (blackleg fungus). These two species are locked in an evolutionary arms-race whereby a gene for gene interaction confers either resistance or susceptibility in the plant depending on the genotype of the plant and pathogen. Two genomic regions on chromosomes A7 and A10 in B. napus are known to contain specific L. maculans-resistance genes. By characterising these genes in canola and assessing the diversity and allelic variation, we can identify sources of resistance genes for use in canola breeding. However, to date, no resistance genes have been sequenced. The newly available Brassica genome sequence and the recent availability of next generation sequencing methods enable the de novo sequencing of Brassica genomes for gene discovery. The aim of the project will be to identify resistance genes within Brassica species and apply this knowledge in the breeding of disease resistant cultivars. 95 6. Building Skills & Capacity GRS10507: Grains Industry Research Scholarship - Reece Tollenaere (UQ) Identification of blackleg resistance genes in canola GRS10512: Grains Industry Research Scholarship - Josh Chopin (USA) Mathematical and Computational Modelling for the Phenotypic Analysis of Cereal Plants GRDC National Region Contract 01/01/2013 Start Contract End Contact Prof. Stan Miklavcic Organisation Phone 19/02/2015 Project Code GRS10512 University of South Australia 08 8302 3788 Email [email protected] Project Summary 6. Building Skills & Capacity Methods for manual phenotypic analysis can be time consuming, labour intensive and often destructive. As such, in large scale genomics experiments they are impractical. This issue has been the catalyst for a worldwide boom in automated phenomics facilities in recent years. However, accurately detecting, segmenting and representing cereal plants in images is a challenging task due to their complicated structure. The narrow, spindly leaves of cereal plants can be self-occluding and twisted, making parts of the leaf as small as one pixel wide in the image. 96 We use two popular image segmentation techniques in order to gain an accurate digital representation of cereal plants. Once an accurate representation is gained we classify the different regions of the plant, such as leaves and stem. Finally, from either the 2D images, or a 3D reconstruction of the plant, we can extract morphological features such as stem height, leaf surface area, biomass etc. While these methods are powerful they are still lacking in a number of areas that require more research to be conducted. Namely, the techniques fail to accurately capture sharp corners in images (leaf tips and axils) and require a number of parameters to be 'handpicked' in order for optimal performance. GRDC Region National Contract 01/01/2013 Start Contract End Contact Professor. Robert Henry Organisation Phone 16/01/2015 Project Code GRS10532 University Of Queensland 07 3346 0552 Email [email protected] Project Summary Necrotrophic fungal pathogens cause diseases of major economic significance to grain crop production in Australia and abroad. Under the GRDC Strategic Research and Development Plan 2012-17, developing effective management strategies for cereal fungal pathogens is a current priority for the Australian grains industry. The development of resistant crop varieties through classical or molecular breeding appears to be the only realistic long-term solution for managing necrotrophic fungal diseases. Brachypodium distachyon is becoming recognized as a highly effective genetic model for grain crops which makes it highly useful as a tool to facilitate understanding of disease interactions between wheat and pathogenic fungi. This project aims to develop a better understanding of the molecular mechanisms of host resistance by utilizing Brachypodium as a model to understand crown rot (CR) in wheat and identify novel disease resistance genes which could have a critical impact on designing effective disease control strategies against necrotrophic pathogens. 1. Initially, available mutant lines (T-DNA insertion and EMS mutagenized) will be screened for resistance to CR. 2. Quantitative RT-PCR and highthroughput RNA-seq will be used to understand gene expression in both plant and fungi during infection and to identify additional candidate genes. 3. The regulation of defence responses by plant hormones jasmonate (JA) and salicylic acid (SA) will be assessed using mutants with impaired JA/SA biosynthesis and reception. 4. Candidate resistance genes will be identified in wheat based on sequence homology to genes identified in Brachypodium. 97 6. Building Skills & Capacity GRS10532: Grains Industry Research Scholarship - Jonathan Powell (UQ) Identifying sources of resistance to necrotrophic plant pathogens using the model grass Brachypodium distachyon GRS10564: Grains Industry Research Scholarship - Alison Renwick (CUR) Effector gene prediction from fungal pathogen genome assemblies GRDC National Region Contract 01/01/2013 Start Contract End Contact Prof. Richard Oliver Organisation Phone 31/07/2015 Project Code GRS10564 Curtin University of Technology 08 9266 4416 Email [email protected] 6. Building Skills & Capacity Project Summary 98 Fungal pathogens interact with their plant hosts largely via the secretion of effectors. The Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) group has shown that these are key determinants of pathogenicity in the Stagonospora nodorum and Pyrenophora Tritici-repentis wheat interactions. It is suspected that effector genes are of importance to the functioning of many of the fungal pathogens that cause crop losses. This has encouraged the ACNFP to sequence other fungal genomes (Pyrenophora Teres, Stagonospora avenae, Ascochyta ssp.) to help to discover effector genes directly from the genomes of these species. Having obtained the genome sequences the next step is to annotate the genes. Unfortunately, the study of effector genes is hindered by the poor success rate of gene finding programs at annotating effector genes. The goal of this project is to understand and if possible improve the process whereby DNA sequences are searched for protein-coding regions, with a particular focus on effector genes. Gene models will be compared to those predicted by experimental data from RNA sequencing and proteogenomics. Discrepancies between the gene models and experimental data will be recorded, and the gene models refined. GRDC National Region Contract 05/06/2013 Start Contract End Contact Prof. Stephen Powles Organisation Phone 04/07/2016 Project Code GRS10566 University of Western Australia 08 6488 7833 Email [email protected] Project Summary Some farmers consistently cope better with the variability that characterises the cropping conditions of the Western Australian grainbelt. an investment strategy for the studied regions will be developed, focusing on the identified drivers of profitability. A consensual idea, shared in the industry by a number of researchers, consultants and growers, is that resilient and profitable farmers employ superior farm management strategies. These strategies are the sum of a unique combination of characteristics including natural resource availability, agronomic practices, technology, skills, flexible and timely management, business and social attitudes. In order to bridge the gap between top performers and the industry average, commonalities within these groups need to be identified. It is too simplistic to assume that structural indicators such as farm size or average rainfall can sufficiently explain consistently higher financial returns among top performers. The diagnostic will evaluate agronomic, economic and other multi-disciplinary indicators, insofar as these contribute to an improved understanding of the decisionmaking process of participating farmers. A systematic method drawing on statistical and non-statistical approaches, surveys of farmers and existing databases will be used to identify key drivers of profitability, prioritise impediments, and compare structural and managerial differences. Emphasis will be placed on gaining first-hand information directly from farmers themselves. The diversity of farm enterprises in the agricultural catchment will be modelled to help determine which issues outstanding performers target (e.g. weed management) and whether trends exist. Consequently, this research will conduct a diagnostic of outstanding performers as well as those at the district average. Subsequently, 99 6. Building Skills & Capacity GRS10566: Grains Industry Research Scholarship - Myrtille Lacoste (UWA) Understanding the strategies of outstanding performers in dryland farming enterprises GRS10577: Grains Industry Research Scholarship - Kirt Hainzer Thesis research into the biological farming approach GRDC National Region Contract Start 01/01/2013 Contract End Contact Mr. Kirt Hainzer Organisation Phone 31/12/2013 Project Code GRS10577 Wageningen University 02 4982 1066 Email [email protected] Project Summary 6. Building Skills & Capacity My thesis research will scientifically analyse the biological farming approach, a farming method which has been successful for a number of wheat farmers in Australia. However, as yet there has not been a scientific study explaining why their practices have been 100 successful. My research will aim to scientifically analyse this promising method, document its key practices and possibly initiate a larger adoption among Australian grain farmers. GRDC National Region Contract 01/07/2013 Start Contract End Contact Ms. Kate Rudd Organisation Phone 31/08/2016 Project Code GRS10578 University of Sydney 93518801 Email [email protected] Project Summary Rust diseases have caused significant losses in Australian cereal crops, and whilst resistance breeding has reduced the frequency of rust epidemics, losses are still experienced in some regions. In Australia, cultivated barley (Hordeum vulgare) is affected by three rusts: leaf rust caused by Puccinia hordei, stem rust caused by P. graminis, and stripe rust caused by a new form of P. striiformis. Leaf rust and stem rust in particular continue to evolve to overcome host resistance genes, and at present, virulence exists for most resistance genes derived from cultivated barley. Following our discovery and characterisation of the first adult plant resistance (APR) gene conferring protection against P. hordei (Rph20), this project will undertake detailed characterisation of several new sources of APR we have identified, including gene interaction, mapping and fine mapping to develop markers to assist in the introgression of the genes into local germplasm. The project forms part of a broader objective of the ACRCP, in providing barley cultivars with durable resistance to leaf rust. 101 6. Building Skills & Capacity GRS10578: Grains Industry Research Scholarship - Paul Kavanagh (US) Genetics of host: pathogen interactions in the Puccinia hordei: Hordeum vulgare pathosystem GRS10658: GRS - Ailisa Blum (UQ) Regulation of the production of the mycotoxin deoxynivalenol by Fusarium graminearum and its according virulence on wheat and barley GRDC National Region Contract 01/01/2014 Start Contract End Contact Mrs. Kaye Hunt Organisation Phone 30/09/2016 Project Code GRS10658 University Of Queensland (07) 3365 3458 Email [email protected] 6. Building Skills & Capacity Project Summary 102 Fusarium crown rot (FCR) and head blight (FHB) cause >AU$100M in losses annually in the Australian industry. There are currently no immune cultivars available for either disease and therefore a greater understanding of the pathogen?s virulence arsenal is required to allow informed design and application of future crop protection strategies. This project will take advantage of the recent advances in pathogen genomics to understand the way in which Fusarium spp. cause disease. Random mutagenesis of F. graminearum will be used to uncover novel mechanisms of virulence, followed by rapid sequence based mapping of the mutation(s). This approach will be a major change in Fusarium research as previously investigations have been largely restricted to analysing selected genes one at a time. The project will focus on the mechanisms underlying how Fusarium spp. regulate the production of the mycotoxin deoxynivalenol. This toxin contributes to pathogen virulence in both FHB and FCR and can contaminate grain products. F. graminearum will be used as this fungus can be crossed easily in the laboratory, an essential feature for mapping mutations. However, ultimately this approach could be taken with other pathogens of importance to the Australian grains industry Rust diseases have caused significant losses in Australian cereal crops, and whilst resistance breeding has reduced the frequency of rust epidemics, losses are still experienced in some regions. In Australia, cultivated barley (Hordeum vulgare) is affected by three rusts: leaf rust caused by Puccinia hordei, stem rust caused by P. graminis, and stripe rust caused by a new form of P. striiformis. Leaf rust and stem rust in particular continue to evolve to overcome host resistance genes, and at present, virulence exists for most resistance genes derived from cultivated barley. Following our discovery and characterisation of the first adult plant resistance (APR) gene conferring protection against P. hordei (Rph20), this project will undertake detailed characterisation of several new sources of APR we have identified, including gene interaction, mapping and fine mapping to develop markers to assist in the introgression of the genes into local germplasm. The project forms part of a broader objective of the ACRCP, in providing barley cultivars with durable resistance to leaf rust. GRS10660: GRS - Eisrat Jahan (US) Genetypic variation in mesophyll conductance of wheat (Triticum aestivum) and its effect on WUE Contract Start 02/12/2012 Contract End Contact Ms. Fiona Lawrence Organisation Phone 02/12/2015 Project Code GRS10660 University of Sydney +61 2 8627 1002 Email [email protected] Project Summary Wheat is one of the most important grains in the world as well as in Australia?s grain industry. It has been predicted that climate change will cause changes in the patterns of precipitation and that these changes will exacerbate existing challenges of water availability for agriculture. Thus, improvements in water-use efficiency are required to maintain and improve wheat yield. The conductance to CO2 diffusion from intercellular airspace to chloroplasts has been termed mesophyll conductance (gm), and significantly limits photosynthetic rate. Increased mesophyll conductance has the potential to improve leaf-level water-use efficiency through increases in photosynthetic rate. For estimating mesophyll conductance I will use gas exchange measurements in combination with discrimination against 13CO2. Our objectives are; to assess existing genetic variation in mesophyll conductance among selected wheat cultivars, to quantify the effect of plant water potential and leaf nitrogen concentration on mesophyll conductance and to determine the physiological basis of variation in mesophyll conductance and its effect on water-use efficiency. 6. Building Skills & Capacity GRDC National Region 103 GRS10661: GRS - Emma Thompson (UQ) Brewer Nutrient recovery from wastewater using electrodialysis GRDC National Region Contract Start 15/07/2013 Contract End Contact Mrs. Vivienne Clayton Organisation Phone 15/07/2016 Project Code GRS10661 University Of Queensland (07)33654680 Email [email protected] Project Summary 6. Building Skills & Capacity Fertiliser price instability, due to increasing global demand, energy costs associated with production and limited resources, has put financial pressure on the Australian agriculture industry. 104 To address this issue, there is a need to assess nutrient resources from wastewater streams and their potential for being processed into high value fertilizer products. A substantial fraction (30-100%) of major nutrients (NPK) can be serviced from wastewater streams in Australia. The project will investigate the application of multi-cell electrodialysis to recover nitrogen and potassium from wastewater streams. The aim is to yield a useful fertiliser product at comparable energetic and economic costs to existing non-renewable sources, with a particular focus on nitrogen and potassium based products. Key challenges include the separation of desired ions (i.e., ammonium, potassium), avoiding scaling, and minimising energy consumption. This will be addressed through mechanistic modelling of the electrodialysis process and demonstrating application of the process using different types of wastewater streams. The mechanistic model is a computer simulation which is based upon first principle theories of electrochemistry and process engineering. Given input conditions, it will be able to predict the efficiency of an electrodialysis process in terms of both the composition of the product and the energy consumption. GRS10664: GRS - Clare Flakelar (UCS) Canola quality - the influence of processing parameters on valuable minor components. Contract Start 17/02/2014 Contract End Contact Professor. Andrea Bishop Organisation Phone 15/11/2016 Project Code GRS10664 Charles Sturt University +61 2 6933 4748 Email [email protected] Project Summary Previously, little research focus has been placed on bioactive components in canola oil. Furthermore, these components (particularly carotenoids, tocoperols and sterols) are reduced or eliminated entirely during current commercial oil production. Many of these compounds are linked to health beneficial effects, e.g. lutein for protection against macular degeneration, and were shown in my 2013 honours project to be present in canola oil in significant amounts, when extracted under mild conditions. Therefore a new project will be undertaken in my PhD to investigate conditions such as temperature, light exposure, addition of solvent etc. and processing techniques (e.g bleaching), and their effects on the concentrations of these components. Furthermore, after discovering that many of these components were strongly influenced by variety in commercial canola samples, the PhD project will investigate GxE effects in a balanced sample set of 64 different geneotypes grown at three field sites in 2013 (from a companion GRDC project conducted at NSW DPI, Wagga). At the beginning of the project I will develop an LCMS/MS method for the simultaneous determination of sterols, carotenoids and tocopherols, to be used throughout the study. Further research will investigate whether a rapid NIR method, such as is used currently in industrial settings, can be developed to determine these compounds in whole seed and/or oil. 6. Building Skills & Capacity GRDC National Region 105 GRS10667: GRS - Fabian Inturrisi (UQ) Identification of candidate genes for blackleg resistance in Brassica Juncea GRDC National Region Contract Start 01/02/2014 Contract End Contact Ms. Kaye Hunt Organisation Phone 01/04/2017 Project Code GRS10667 University Of Queensland 07 33653458 Email [email protected] 6. Building Skills & Capacity Project Summary 106 Brassica species are important crops producing cooking oils, vegetables, condiments and biofuels, grown in diverse environments, with high economic and export value. A thorough understanding of the relationships between plants and pathogens is essential if we are to continue to meet the agricultural needs of the world’s growing population. canola breeding for blackleg disease resistance by identification, characterisation and assessment of diversity and allelic variation of candidate resistance genes among B. juncea lines. The newly available Brassica genome sequence and the recent avaliability of next generation sequencing methods enable the de novo sequencing of Brassica genomes for gene discovery. Leptosphaeria maculans (blackleg fungus) is a devastating fungal pathogen for canola in Australia. L. maculans and Brassica species are locked in an evolutionary arms-race whereby a gene for gene interaction confers either resistance or susceptibility in the plant depending on the genotype of the plant and pathogen. Brassica juncea is important in The aim of this project will be to add to the growing genomic knowledge of Brassicas, through the identification of candidate resistance genes in B. juncea against L. maculans along with applying this knowledge in the breeding of disease resistant cultivars. GRDC National Region Contract 02/01/2014 Start Contract End Contact Ms. Gagan Bhardwaj Organisation Phone 08/01/2018 Project Code GRS10683 Australian National University +612 6125 9395 Email [email protected] Project Summary For this project, I am particularly interested in researching the involvement of epigenetic mechanisms in enhancing the adaptive ability of plants to their environment and the associated stresses, and in particular elucidating the particular epigenetic mechanisms that may be involved. This project is aimed at confirming that plants harbor the ability to have a ‘memory’ of past stresses that are passed down through generations such that later generations are ‘primed’ to thrive in those growth conditions. Currently, it is largely unknown the extent to which epigenetic mechanisms can effect the phenotypes of plants, including yield produced by crop species. In particular, I want to establish the impact that the environment has in influencing plant phenotypic traits, specifically through epigenetic mechanisms. For instance, we may be able to influence the resulting phenotype of a plant by controlling the conditions that the parental seed stock are exposed to. This project is also aimed at exploring which genes are targeted by such epigenetic marks, such that this project may contribute to the understanding of plant epigenetics at a molecular/mechanistic level. For this project, I will begin by investigating which of the following plant organisms: Arabidopsis thaliana or Brachypodium distachyon, will be most suited for my project. Additionally, I am also concerned about having a consistent and reliable application of stress; therefore, the early stages of this project will involve optimizing the experimental design. For this project, I am considering using excess light, heat, drought, fluctuating light or a combination of these as forms of stress to apply to plants. The current major aspect for this project involves applying the chosen stress-treatment to the chosen plant species over multiple generations (at least 10 generations) and observing for the transgenerational inheritance of epigenetic marks. 107 6. Building Skills & Capacity GRS10683: GRS - Diep Ganguly (ANU) What are the epigenetic mechanisms involved in regulating stress response in Arabidopsis thaliana and Brachypodium distachyon GRS10686: GRS - Jessica Mackay (UA) Biological farming systems: is there a role for mycorrhizas and organic amendments in the grain industry? GRDC National Region Contract 02/01/2014 Start Contract End Contact Ms. Andrea Przygonski Organisation Phone 30/11/2016 Project Code GRS10686 University of Adelaide +61 8 8313 4739 Email [email protected] Project Summary 6. Building Skills & Capacity As nutrients become increasingly scarce and expensive, we need to find innovative ways to supply grain crops with the nutrients they need in order to maximise production. Equally, we need to ensure that the nutrients we apply are available to plants and are not lost to the environment via leaching or as greenhouse gasses such as nitrous oxide. 108 If we are to achieve these goals, we need to identify: ? Sustainable new options for supplying nutrients to our crops ? Soil biological processes that help release nutrients to plants when they are needed most and ? The processes that maximise the capacity of plants to acquire nutrients In this project I will investigate the potential for biological farming systems to be applied to mainstream grain production systems. I will focus on the potential to supply nutrients to crops as organic amendments, and the role of mycorrhizas in acquiring those nutrients. The project will involve a combination of laboratory and glasshouse based studies complimented with on-farm surveys and experiments. A wide range of analytical techniques will be used to generate currently lacking knowledge, that is both scientifically cutting edge, and farm-relevant. Techniques used will include those used in other GRDC programs, such as the soil biology initiative. GRS10687: GRS - John Rivers (ANU) RNA secondary structure and the regulation of metabolism Contract Start 31/03/2014 Contract End Contact Ms. Patricia Seddon Organisation Phone 31/03/2017 Project Code GRS10687 Australian National University 61261259090 Email [email protected] Project Summary The ability to control the activity of specific genes (AKA ‘gene expression’) is central to plant biotechnology. A key determinant of gene expression within cells are the intermediary, polymeric molecules known as ribonucleic acid (RNA), which contain information encoded by an organism’s genome. RNA molecules are known to fold back on themselves, forming secondary structures known as ‘riboswitches’ and ‘thermometers’. I am interested in developing metabolic screening technology to identify regulators of signalling pathways that control natural product accumulation (e.g. carotenoids) in grains such as wheat. Recent work in the Pogson Lab at ANU has revealed that a carotenoid biosynthetic ?-cyclase (?LCY) gene, is regulated by cis-carotenoid accumulation and an unknown derivative is hypothesised to alter the structure of the ?LCY RNA, thereby affecting gene-expression. In bacteria RNA can affect the ability of cellular enzymes to access the information encoded in RNA, thus regulating gene expression and protein activity. The discovery of RNA regulatory processes that are controlled by chemical application will pave the way to enhance natural productenrichment in grains and lead to new commercial ventures. This proposal will investigate RNA regulatory mechanisms controlling metabolic accumulation in plants. Plants are natural chemical factories. 6. Building Skills & Capacity GRDC National Region 109 GRS10690: GRS - Kenton Porker (UA) Manipulating and understanding barley phenology to maximise yield potential GRDC National Region Contract Start 03/10/2014 Contract End Contact Ms. Andrea Przygonski Organisation Phone 03/09/2017 Project Code GRS10690 University of Adelaide (08)83134739 Email [email protected] Project Summary 6. Building Skills & Capacity The combination of genetics involved in grain phase development and flowering date (phenology) is a major determinant of the adaptation of barley to different environments. Flowering time during the 'optimal window' is critical to maximise yield and minimise the risk of frost, heat, and drought stresses. Improvements in barley genomics have identified diversity in major development genes known to control phenology, including vernalisation requirement, photo-period sensitivity, and inherent earliness. Current understanding of phenology-gene combinations, interactions with the environment and yield components is limited. 110 This PhD intends to align with the GRDC strategic research and development plan surrounding agronomy and crop physiology and the 2014 GRDC investment plan ‘manipulating barley phenology to maximise yield potential’ involving collaboration with the University of Adelaide, CSIRO, and the University of New England. Based on field and laboratory validation, this project aims to: a. provide understanding of the relationship between phenology genes and yield components to identify the optimal phase development pattern for barley b. link gene sequence information with field phenotyping to better understand how gene combinations impact phenology c. assist development of a gene-based phenology model which would enable breeders to better target optimal gene combinations for different production environments using parameters from a smaller set of phenotypes Growers are increasingly sowing earlier without knowledge of varietal developmental responses. Part of this project therefore aims to characterise, assess, and simulate the suitability of current genetics for earlier sowing in Australian farming systems across a range of environments using crop management tools such as APSIM. GRDC National Region Contract 01/01/2014 Start Contract End Contact Ms. Leanne Pooley Organisation Phone 04/04/2016 Project Code GRS10691 University Of Queensland (07)54601342 Email [email protected] Project Summary The objectives of this study are to gain an understanding of the biology of Neocosmospora vasinfecta, an emerging soilborne pathogen of peanuts that causes severe root rot, and to develop accurate phenotypic screening techniques to identify sources of resistance to the pathogen Neocosmospora root rot has been found in all peanut-growing regions in Australia, in recent years causing between 10 and 50% crop and economic losses leading to reduced plantings in Central Queensland, Bundaberg and the Burnett regions. Whilst there is much literature on the ecology and biology of other soilborne diseases of peanuts, there is a paucity of information available in the global literature concerning Neocosmospora infection in peanuts. The project will therefore initially concentrate on understanding the biology and epidemiology of Neocosmospora root rot, as a prelude to developing a phenotypic screening tehcnique for potential use in the Australian peanut breeding program, based at Kingaroy, Queensland. Rapid screening techniques for this SBD under controlled conditions offers plant breeders the opportunity to achieve early generation selection and hence save precious time and resources. There is also the potential to develop molecular markers for these traits, via collaboration with an allied UQ PhD program (Mr Dan O'Connor) being conducted at DAFF Kingaroy. 111 6. Building Skills & Capacity GRS10691: GRS - Kylie Wenham (UQ) Investigations into the biology and management of Neocosmospora root rot of peanuts in Australia. GRS10692: GRS - Nathan Craig (UWA) The influence of cereal monoculture and high crop residue levels on mineral nitrogen availability under long term no-tillage systems. GRDC National Region Contract 03/04/2013 Start Contract End Contact Mr. Alan Luks Organisation Phone 30/06/2016 Project Code GRS10692 University of Western Australia 08 6488 2541 Email [email protected] Project Summary 6. Building Skills & Capacity The application of artificial nitrogen fertiliser to maximise crop yield represents a significant cost to farm production. In the low rainfall zone of Australia, this is exacerbated by the adoption of cereal monoculture as the most profitable sequence of crops to be grown. 112 This project will explore the influence of crop rotation and the adoption of high-crop-residue no-tillage systems on labile soil carbon and plant available soil nitrogen. A key focus of this project is to determine if cereal monocultures can become less dependant on artificial nitrogen fertiliser through long-term changes in soil microbiology. An established long-term no-tillage trial site with cereal monoculture and diverse crop rotation including legumes will be used to measure nitrogen mineralisation, changes in soil microbiology, and changes in soil organic carbon under each crop rotation. The identification of the gains and losses of soil nitrogen under each crop rotation will be further investigated to better understand the factors affecting seasonal changes in soil nitrogen under a high-crop-residue environment. The Agricultural Production Simlulation model (APSIM) will be calibrated using the results from this trial to predict the long-term changes in crop yield from changes in soil nitrogen and organic carbon. GRS10694: GRS - Sandra Kerbler (UWA) Investigating the molecular basis of thermal acclimation in plant mitochondria Contract Start 25/03/2013 Contract End Contact Mrs. Jenny Gillett Organisation Phone 25/03/2016 Project Code GRS10694 University of Western Australia 08 64884416 Email [email protected] Project Summary Fluctuations in temperature can profoundly impact plant growth and development. Although crop plants have the ability to acclimate to different temperatures, this has a major impact on the energy efficiency of crop production. As temperatures in agricultural regions are becoming increasingly unpredictable, knowledge of how plant growth responds to different temperatures is vital if we are to maintain current levels of crop productivity. Mitochondria play a key role in determining the rate of plant growth as actively functioning mitochondria produce much of the energy and carbon skeletons necessary for biosynthesis and cellular maintenance during plant respiration. Mitochondrial function in flowers is also critical for fertility and pollen production, linking respiratory dysfunction and infertility of crops during temperature extremes. Respiratory processes are very conserved across plant species and therefore study in model plant species has broad applicability to many crop species. Recent research has revealed that changes in mitochondrial proteins have a key role in plant thermal tolerance by maintaining ATP production. However, the entire suite of proteins, the degree and mechanism by which they change and how this process can be modified to enable acclimation of respiration, is yet to be fully understood. Therefore this PhD project aims to identify how molecular changes within the mitochondria allow plants to continue growth at a range of temperatures. 6. Building Skills & Capacity GRDC National Region 113 GRS10695: GRS - Sarah Lorberg (UQ) Novel sources of disease resistance in Brassica GRDC Region National Contract 10/01/2013 Start Contract End Contact Ms. Kaye Hunt Organisation Phone 10/01/2016 Project Code GRS10695 University Of Queensland 07 33653458 Email [email protected] Project Summary The study of plant interactions with microbes, both beneficial and pathogenic, has important implications for improving plant productivity. Plants require mechanisms to recognise both types of microbe and respond accordingly. 6. Building Skills & Capacity This project will investigate the role of Brassicaceae SYMBIOSIS (SYM) genes in pathogenicity, as they appear to form part of an ancient microbial recognition pathway. Specifically I aim to discover if any of the known SYM genes are involved in pathogenic interactions, including between canola and the devastating disease, blackleg, caused by the 114 fungus Leptosphaeria maculans. In parallel, my PhD aims to further understand the general biology of L. maculans and particularly the molecular and genetic changes that occur during in vitro loss of pathogenicty following rounds of continued subculturing. Additionally, I will characterise molecular differences between L. maculans and its relative L. biglobosa , which is extremely aggressive in the early infection stages but does not form major disease symptoms or crop losses. GRS10698: GRS - Thomas Simnadis (UW) Studies in Novel Grains for the Australian Food Supply Contract Start 03/03/2014 Contract End Contact Mr. Peter Roope Organisation 30/11/2016 Project Code GRS10698 University of Wollongong Phone Email Project Summary The research will focus on the Australian grains industry and explore the possible incorporation of ancient grains such as amaranth , buckwheat, millet, sorghum, spelt and teff into the Australian food supply. The health benefits of consuming these grains will be explored through a systematic literature review while econometric modelling will be undertaken to explore factors which may promote or inhibit the adoption of these grains in the Australian agricultural industry from an environmental and economic perspective. It is anticipated that this focus will be complemented with preliminary investigations into the possible introduction of ancient grains into an overseas market such as the United States through pre-existing research linkages. 6. Building Skills & Capacity GRDC National Region 115 GRS10699: GRS - Vijira Wanniarachchi (UF) Alternative respiratory genes can improve tolerance to abiotic stresses in cereals GRDC National Region Contract 02/11/2013 Start Contract End Contact Associate Professor. Kathleen Soole Organisation Phone 02/10/2017 Project Code GRS10699 Flinders University 08 8201 2030 Email [email protected] Project Summary 6. Building Skills & Capacity Abiotic stresses such as drought, salinity, extreme temperatures and chemical toxicity, cause significant annual yield reductions worldwide. Normal growth and development of plants is dependent on the capacity of plants to overcome these stresses, and this capacity has enormous significance in crop yield. Tolerance mechanisms in plants are controlled by genes, and, once the controlling genes have been identified, they can be manipulated to develop improved crop varieties using biotechnological tools. 116 The alternative respiratory pathway associated with the mitochodnrial electron transport chain helps to reduce the production of reactive oxygen species, thus alleviate growth defects from oxidative stress. The main proteins in this pawthay are alternaive oxidase (AOX) and alternative dehydrogenases (NDH). It has been shown that increased level of expression and activity of AOX and NDH, can improve biomass growth of the model plant, Arabidopsis by 30-40% under stress conditions that induce oxidative stress, such as salinity, and combined heat, drought, and high light. I wish to extend these observations to the model cereal, rice. I am using two approaches; 1. screening already tolerant rice varieties for the expression of AOX and NDH genes and 2. producing transgenic lines with upregulated levels and assessing their tolerance to environmental stress and impact on seed yield. GRS10780: GRS - Adam Taranto (ANU) Compontents of Immunity to Stagonospora nodorum in Wheat Contract Start 03/01/2014 Contract End Contact Ms. Amelia Irby Organisation Phone 03/01/2017 Project Code GRS10780 Australian National University +61 2 6125 9394 Email [email protected] Project Summary Stagonospora nodorum is the causal agent of septoria nodorum blotch (SNB) of wheat and is estimated to cost Australian growers $108 M per annum. Recent whole genome sequencing of S. nodorum has enabled the identification of necrotrophic effectors (NEs) specific to virulent races of the pathogen (e.g. ToxA, Tox1 and Tox3). These effectors alone cause necrosis on susceptible hosts and are critical for disease. The Solomon lab has shown that, paradoxically, these effectors also induce a very strong host defence response that S. nodorum must either evade or suppress to cause disease. I will use deep RNAsequencing to profile the response of susceptible and resistant wheat varieties to S. nodorum during infection. These data will reveal how S. nodorum copes with plant defence and will also aid in identifying host genes involved in disease resistance. In a complementary approach, I will scan my RNA sequencing data to seek small RNAs secreted by S. nodorum during infection. Recent studies have shown that these secreted small RNAs interfere with host defence enabling rapid disease progression. I plan to functionally characterise the identified wheat genes using a viral-induced gene silencing approach through the collaboration of the Solomon lab with the Rothamsted Research Station (UK). 6. Building Skills & Capacity GRDC National Region 117 GRS10781: GRS - Jayachandra Rongala (UA) Cereal Transport Proteins Involved In Boron Toxicity Tolerance: How Natural Variation And Sequence Diversification Relate To Structural And Functional Properties GRDC Region National Contract 01/01/2014 Start Contract End Contact Dr. Monica Ogierman Organisation Phone 31/01/2016 Project Code GRS10781 Australian Centre for Plant Functional Genomics 8 8303 6725 Email [email protected] Project Summary 6. Building Skills & Capacity Soil toxicity due to a high subsoil concentration of boron and related metalloids is a constraint influencing cereal crop production many regions of the world. Toxicity effects grain production through reduced yield and quality through accumulation of metalloids that can cause intoxication of both humans and animals. Amelioration of high concentrations of metalloids from agricultural soils is not practical. 118 Breeding tolerant varieties remains the only feasible strategy to address the problem, and it is imperative that research is directed towards understanding boron transport protein function so that the identified naturally occurring variation for tolerance can be effectively deployed in breeding. Recent discoveries of the barley and wheat transport proteins such as boron transporter (HvBot1, TaBot-B5b) and multifunctional aquaporins (HvNIP2;1) have advanced our knowledge of the genetic control of boron tolerance and provided new opportunities to address the problem. In my PhD study these proteins from barley and wheat will be functionally characterised to further our understanding of the relationship between tolerance allele sequence and functionality in the plant. This information will support the implementation of novel and naturally occurring allelic variation in breeding. GRDC Region National Contract 01/01/2011 Start Contract End Contact Dr. Lambert Brau Organisation Phone 31/12/2013 Project Code GRS187 Murdoch University +61 3 9251 7055 Email lambert.brau@deakin .edu.au Project Summary The ability of legume plants to fix atmospheric nitrogen and make it availabel is the result of a symbiotic relationship between the host plant and soil bacteria know as rhizobia. The rhizobia occupy structures (known as nodules) on the root of platns ans it is within these structures that they are able to convert atmospheric N2 to NH3+. It has been shown that the formation of nitrogen fixing nodules on grain legumes (such as lupins) or pasture legumes (such as clover) can be enhanced by co-inoculating with specific plant growth promoting rhizosphere bacteria (PGPRs). Increased number of nodules can increase the amount of atmospheric N2 fixed and thus enhance plant growth. Preliminary work suggests that the increase in nodulation is not as a result of a change in root physiology of the host plant but may be caused by an exchange of molecular signals between the PGPR and the rhizobia, the PGPR and the plant or the PGPR with both. This project will aim to elucidate if such signalling is occurring and if so, to determine the nature of these signal molecules. The second aim of the project will be to establish whether the PGPR are able to colocate with the rhizobia in the nodule structures ow whether they remain outside the plant tissue, in the rhizosphere. 119 6. Building Skills & Capacity GRS187: Grains Industry Research Scholarship - Liza Parkinson (UMU) Investigating the method of action of plant growth promoting Rhizosphere bacteria enhancing nodulation in legumes GTA10022: Travel Award - Mr Pradeep Sornaraj (UF) The XV Congress on Molecular PlantMicrobe Interaction/Presenting data at the Conference GRDC National Region Contract 01/08/2011 Start Contract End Contact Dr. Peter Anderson Organisation Phone 13/08/2012 Project Code GTA10022 Flinders University 08 8201 5269 Email [email protected] 6. Building Skills & Capacity Project Summary 120 1. The conference will provide me with an opportunity to meet the research community working on common goal to improve the means of controlling pathogen mediated loss to the grain industry. 2. The conference will enable me to identify new techniques that could be useful in understanding the role of different domain in the plant resistance R protein and the importance of nucleotide binding leading to the activation of resistance R proteins. 3. It may help in developing new research collobrations between research laboratories within Australia and all over the world. 4. The conference will act as a mean to transfer knowledge and will improve the general understanding of the biology behind pathogen/plant interactions among industry collaborators and plant researchers 5. I will also be able to demonstrate the participation and commitment of the GRDC to support and nurture fundamental research in plant/pathogen interactions towards improving the management of pests and diseases to grain growing industry. GTA10267: Travel Award - Julia Brueggemann (UA) Plant & Animal Genome XX 121 GRDC Region National Contract End Contact Prof. Diane Mather Organisation Phone 20/01/2012 Project Code GTA10267 University of Adelaide 08 8313 7156 Email [email protected] Project Summary Information on and implementation of new molecular markers for traits of interest to Australian wheat and barley breeders and to pre-breeding research. Information on novel molecular marker techniques whose adoption has the potential to reduce the cost of marker assisted selection and/or of pre-breeding research. Information on new wheat and barley genomics data resources and on the related bioinformatics tools that can accelerate molecular marker development. 6. Building Skills & Capacity Contract 13/01/2012 Start GTA10348: Travel Award - Maarten Kooiker (CSP) International Plant Molecular Biology Congress GRDC National Region Contract Start 30/06/2012 Contract End Contact Mr. Gang-Ping Wynne Organisation Phone 28/10/2012 Project Code GTA10348 CSIRO Plant Industry 07 3214 2354 Email Project Summary 6. Building Skills & Capacity At the conference in Korea several groups, including leading researchers in the field will present their data and recent findings. Since many of these presentations will cover research topics closely related to my research, I will be learning from these researchers and their approaches and/or new techniques. It will also give me the opportunity to contact these researchers and exchange ideas and possibly 122 establish collaborations. New information about the proceedings in the of colleagues working in the same research field will impact future research strategies that aim to increase yields of wheat in terminal drought conditions and the adaptation to climate changes. GTA10361: Travel Award – Alexandra MilnerSmyth (SAN) SANTFA Bus Trip to New South Wales Contract Start 16/06/2012 Contract End Contact . Alexandra Milner-Smyth Organisation Phone 20/07/2012 Project Code GTA10361 South Aust. No-Tillage Farming Ass. Inc. (SANTFA) 888424278 Email [email protected] Project Summary The trip aims to build on the existing knowledge of South Australian farmers by exposing them to new methods and technologies with an emphasis on releavence to SA conditions and ease of implementation. By educating farmers in the eareas of subjects including (but not limited to) permanent ground cover, minimum / zero-tillage, soil health, biodiversity, reduction of herbicide resistance and crop rotations, SANTFA aims to assist growers to reduce their inputs while increasing the quality and quantity of grains. SANTFA also seeks to show attendees how to manage stock in a conservation agriculture or no-till system to minimise financial risks during drought or disease. 6. Building Skills & Capacity GRDC National Region 123 GTA10368: Travel Award - Matthew Gilliham (UA) Conference Travel to US and visits researchers to strike up collaboration GRDC National Region Contract Start 20/06/2012 Contract End Contact . Chelsea DuBois Organisation Phone 01/08/2012 Project Code GTA10368 University of Adelaide 883137116 Email [email protected] Project Summary 6. Building Skills & Capacity The purpose of my trip is two fold, to form international collaborations and to showcase the capacity for high-impact salinity tolerance research in cereals in Australia. In terms of collaboration I will build relations with two of the leading research institutes in the area of abiotic plant stress tolerance, UCSD and the DanForth Centre, and an industrial giant that is 124 already beginning to invest in the Australian wheat industry through Intergrain. It is important that the results of our high-profile paper, resulting from a collaboration between several Australian Institutes with support from the GRDC, is desiminated in such a highprofile international forum. GTA10385: Travel Award – Sarita Bennett (CUR) To present a paper at the 16th Australian Agronomy Conference Contract Start 30/06/2012 Contract End Contact . Belinda Northeast Organisation Phone 18/10/2012 Project Code GTA10385 Curtin University of Technology 892664248 Email [email protected] Project Summary My research on abiotic stresses has been based in Western Australia with limited collaboration with researchers in the eastern states. Through attendance and presenting at the Agronomy Conference I am to meet new researchers working in a similar area where we can discuss current research aims and future collaboration. This will ensure that current research is applicable to both eastern and Western Australia, thus increasing its benefit to the Australian grains industry and ensure that future work is applicable to both eastern and Western Australia. Presenting the work on boron and salinity tolerance in field peas will communicate the results to the wider community as the techniques used can be applied across different crop and pasture varieties. 6. Building Skills & Capacity GRDC National Region 125 GTA10419: Travel Award - Bo Xu (UA) International Workshop on Plant Membrane Biology XVI 2013 GRDC National Region Contract Start 24/03/2013 Contract End Contact Dr. Matthew Gilliham Organisation Phone 05/04/2014 Project Code GTA10419 University of Adelaide 08 8303 8145 Email [email protected] Project Summary 6. Building Skills & Capacity By travelling to and presenting at IWPMB2013 I will have the opportunity to describe my results demonstrating the discovery of two natural sources that confer Na-exclusion in wheat. My work, which is part of a national collaboration with CSIRO and ACPFG not only demostrates that Australian wheat production can be improved on saline soil but also has drawn notable international attention from both the scientific community and the general media, with over 500 media stories. It demonstrates the importance of exploring natural variation for improving abiotic stress 126 tolerance and yield in the field. Meanwhile my comparison of the properties of different Natransporter variants will provide a guide to breeders for selecting candidates for improving salt tolerance of wheat and other cereals. Additionally, visiting Ma’s and Uozumi's labs will provide an opportunity to improve international collaboration in this area with leading overseas laboratories who also explore cereal abiotic-stress tolerance. GTA10422: Travel Award - Kaitao Lai (UQ) PAG XXI Conference 127 GRDC Region National Contract End Contact Dr. Owain Edwards Organisation Phone 18/01/2013 Project Code GTA10422 University Of Queensland 08 9333 6401 Email [email protected] Project Summary My unpublished work is focusing on characterize genetic diversity in 16 Australian bread wheat varieties. I expected to exchange the latest research ideas related to characterizing genetic diversity between varities from the coference. These experiences from other research group could help me to characterize genetic diversity in Australian bread wheat varieties to enable the generation of superior genotypes for crop imovement and producitivity. 6. Building Skills & Capacity Contract 10/01/2013 Start GTA10427: Travel Award - Roslyn Jettner (DAW) Understanding the China market and its requirement for Milling Oats - Industry Champion travel GRDC National Region Contract 30/06/2013 Start Contract End Contact Ms. Roslyn Jettner Organisation Phone 30/06/2013 Project Code GTA10427 Department of Agriculture and Food Western Australia (DAFWA) 08 9368 3241 Email [email protected] 6. Building Skills & Capacity Project Summary 128 The growth in development of new consumer products with health benefits and in oat processing facilities in China represents a major opportunity for the growth of milling oat exports from Australia. capture future growth in the Chinese market. It will create an environment to establish relationships between the Australian industry and key Chinese oat researchers, processors and manufacturers. This visit will provide intelligence on the oat quality characteristics that are required for the production of traditional and new nontraditional Asian food products for the oat breeding program. This market tour will clearly define the China oat opportunity and how the Australian oat industry may position itself to The Australian oat brochure will promote the benefit and quality characteristics of Australian oats aimed at differentiating our grain from alternative sources in order to be the supplier of choice with the objective of returning greater value to Australian oat growers. GTA10428: Travel Award - Sandra Schmoeckel (UA) International Workshop on Plant Membrane Biology XVI 2013 Contract Start 25/03/2013 Contract End Contact Dr. Rob Harris Organisation Phone 02/04/2013 Project Code GTA10428 University of Adelaide 03 5573 0963 Email [email protected] Project Summary I am a member of the Salt Focus Group under the supervision of Dr Stuart Roy, Dr Bettina Berger and Prof Mark Tester. Our research is targeting the understanding of salinity effects on plants and to improve the plants? salinity tolerance, in particular crop plants. Abiotic stress caused by salinity is a major threat to agriculture, resulting in reduced crop yields and endangering food security. With a continuously increasing land area affected by salinity, the understanding of the molecular mechanisms concerning salt affected plants is of heightened importance. Particularly important to improve the plant’s tolerance to salt (sodium) stress is to understand how sodium is being taken up by the plant and transported throughout the plant. These processes are facilitated by transporters located at the various membranes of each plant cell and generally tightly regulated. In my PhD project I am analysing the sodium transporter HKT in the model plant Arabidopsis, with the aim to understand the regulation of sodium transport within the plant. This knowledge could then be transferred to crops like wheat and barley. As a successful example I would like to mention the Arabidopsis gene CIPK16, which was used to improve salinity tolerance in barley (Roy et al. 2012). I was involved in the characterisation of the gene in Arabidopsis, which demonstrated to not only improve salinity tolerance in the model system Arabidopsis, but also in the cereal crop barley. The International Workshop on Plant Membrane Biology would be a great opportunity to get to know new cutting edge technology that is not used within the University of Adelaide and to discuss my research and moreover my ideas with scientist in that field. I will have the chance to network towards establishing collaborations for my work. As the conference in the final year of my PhD studies, I anticipate to gain valuable information which I can relate to the direction of my research before completing my PhD in end-2013. 6. Building Skills & Capacity GRDC National Region 129 GTA10430: Travel Award - Stephen Carr (Aglime) Gain a more detailed understanding of techniques used in encouraging farmers to apply adequate lime to remove soil acidity GRDC National Region Contract 01/07/2013 Start Contract End Contact Mr. Stephen Carr Organisation Phone 01/08/2013 Project Code GTA10430 Aglime of Australia 892775529 Email [email protected] Project Summary 6. Building Skills & Capacity My aim is to meet with several lime supply companies, farmers and farm advisers. I am interested to meet both technical people and those involved in marketing, using and recommending aglime. 130 I represent the whole lime industry in WA (I am currently the Deputy Chair of Lime WA Inc, a voluntary code of practice, whose members supply in excess of 80% of all aglime sold in WA). I have held this role for the past 3 years, and our members have largely based their marketing approach around the technical need to apply aglime, but this approach seemingly has flaws that prevent us from convincing all farmers they need to apply aglime at the rates DAFWA recommends. In the specific business I manage, we have a large and ongoing field trial program; this enables us to show farmers long term and ongoing aglime responses, but even in the most favourable seasons, farmers still only apply about 30% of the aglime they need to. We need to understand other factors that influence farmers decision making, and speaking to aglime suppliers, marketing people, farmers and their advisers that are involved in agriculture where aglime use is routine will enable these approaches to be adopted to increase aglime use in WA. Soil acidity is a major land degradation issue in WA, conservatively costing 500 million dollars per annum in lost production. The application of aglime removes soil acidity as a constraint, but farmers in WA do not apply adequate lime. The Lime industry in WA needs to understand how other factors (other than yield responses and economic gain) influence farmers to not apply lime that is essential for sustainable farming. The use of aglime in WA has only really been for about 30 years, despite the clear scientific facts about the need for this process. In contrast, lime has been used for hundreds of years in other parts of the world, and possibly thousands of years. We need to understand how better to be able to influence farmers to apply what is required. Our approach has been to conduct long term field trials, that show marked yield response to the use of aglime.I worked as a soil researcher for nearly 20 years, and in a commercial setting for 10 years. Much of my early career was dedicated to understanding the chemical impact of soil acidity (eg aluminium toxicity). As Deputy chair of Lime WA Inc, I represent the whole industry in WA, and have an unrivalled blend of technical and commercial experience in identifying and treating soil acidity. There is adequate aglime available in WA to remove soil acidity as a constraint to productive agriculture. If soil acidity did not exist in WA, conservative estimates suggest this would add 500 million dollars annually to the economy. Aglime was virtually not used in WA 30 years ago, and although use in WA has increased from a very low base, it is still not routine for many farmers. Possibly as many as 50% of farmers have not applied any aglime. By meeting with people that have been involved in supplying and marketing and using aglime in South Africa, USA and Chile, where it has been far more routine for many hundreds of years will help me to help the WA Lime industry and directly the wider Australian farming community. 131 6. Building Skills & Capacity GTA10475: Travel Award - Michael Nash (UM) Attend conference and gain knowledge of current slug control research GRDC National Region Contract Start 01/09/2013 Contract End Contact Ms. Emily Thomson Organisation Phone 15/09/2013 Project Code GTA10475 University of Melbourne 61383442522 Email [email protected] Project Summary 6. Building Skills & Capacity By presenting results from Australian research I will communicate problems associated with slug species adapted to drier and warmer enviroments. Feedback and meetings to be organised will build links to European experts studying these pests. Advice / insights gained on the implications of Milax and the new invasive species and why they are becoming more dominant is directly relevant to the Australian grains industry, as these species are the least studied but most damaging. 132 Establishing links with international collaborators will enhance capacity that could inform control guilines. Investigating how European canola growers control slugs and what bait technologies are being used to reduce environmental loading will limit likely environmental impacts in Australian farming systems and improve control outcomes using existing molluscicides. GTA10498: Travel Award - Iain Searle (ANU) Keystone Conference- RNA Silencing 133 GRDC Region National Contract End Contact Mr. Iain Searle Organisation Phone 18/03/2013 Project Code GTA10498 Australian National University 02 6125 2322 Email [email protected] Project Summary Canola, an important Australian oil seed crop, has significant yield reduction due to biotic (e.g. Blackleg disease) and abiotic stresses (e.g. drought and frost). My laboratory has developed an innovative method to allow widecrosses to suceed therefore allowing introgression of novel agronomically important genetic variation from wild relatives into crop plants. While we have successfully achieved this within the Brassicaceae, cereals are our next goal. Achieving routine wide-crosses within the cereals would be of immense benefit to the Australian grains industry. A deeper understanding of the mechanism underlying our novel discovery involing non- coding RNAs and hybridization barriers would increase the chances of successful application for our technology to cereals. An expected outcome from this research would be producing pre-breeding genetic material with enhanced genetic variation (e.g. crown rot resistance) for breeding programs (e.g. durum wheat breeding program). Furthermore during this proposed trip, I intend to establish an international collaboration with Lars Ostergaard (UK) to translate our discovery to canola via a non-transgenic route. 6. Building Skills & Capacity Contract 17/03/2013 Start GTA10499: Travel Award - Jiaen Qiu (ACPFG) International Workshop on Plant Membrane Biology XVI 2013 GRDC National Region Contract Start 22/03/2013 Contract End Contact Dr. Stuart Roy Organisation Phone 16/04/2013 Project Code GTA10499 Australian Centre for Plant Functional Genomics 08 8303 7159 Email [email protected] Project Summary Presenting at IWPMB2013 will give me the opportunity to describe my results to other researchers and obtain more valuable information which will help me further understand the role of Cl- transporters in salt tolerance. 6. Building Skills & Capacity The ultimate goal of my research is to apply 134 the genetically Cl- transport information to the Australian crop plant, such as wheat and barley. It is well knowledge that regulates Cltransport within crop plant is also an important method to improve the crop production under saline conditions. GTA10511: Travel Award - Wenmian Huang (ACPFG) International Workshop on Plant Membrane Biology XVI 2013 Contract Start 22/03/2013 Contract End Contact Dr. Stuart Roy Organisation Phone 16/04/2013 Project Code GTA10511 Australian Centre for Plant Functional Genomics 08 8303 7159 Email [email protected] Project Summary The information obtained from the conference will help to improve my PhD work and further my career. Due to the diverse mechanisms involved in plant salinity tolerance, a candidate gene needs to be investigated at genomic, transcriptional, translational and posttranslational levels before breeding and genetic modification. My PhD project focuses on the investigation the role of the CIPK16 as a protein kinase in plant salinity tolerance. AtCIPK16 was recently identified by positional cloning as an important gene reducing shoot Na+ accumulation and increasing plant salinity tolerance (Roy et al., 2012, PCE DOI:10.1111/j.13653040.2012.02595.x). Before PhD project, my master project focused on characterisation of AtCIPK16 gene and this project heavily contributed to a recent publication from the laboratory (Roy et al., 2012, PCE DOI:10.1111/j.13653040.2012.02595.x). Transgenic Arabidopsis constitutively expressing AtCIPK16 has significantly reduced shoot Na+ (Roy et al., 2012). After transferring this gene into barley, transgenic barley constitutively expressing AtCIPK16 shows increased salinity tolerance, as measured by biomass production under salt stress in greenhouse conditions (Roy et al., 2012). Field trials of transgenic barley constitutively expressing AtCIPK16 are currently undergoing yield evaluations in the saline field in WA. New technology presented in this conference will provide me more technical support and potential approaches to fully examining my candidate gene identified to increase salinity tolerance of plants. After characterisation of gene and understanding relevant cellular processes, by using genetic engineering to introduce salinity tolerance traits into cereal and to manipulate these mechanisms in crop plants to create new varieties which can survive and produce higher yields on saline soils and then eventually to enhance the productivity of Australian grain industry. Therefore, insights gained from the conference on plant breeding session are going to help me and my lab with the delivery of the knowledge from my PhD project to Australian cereal breeding, genetic engineering projects, which then could benefit the grain industry of Australia. 6. Building Skills & Capacity GRDC National Region 135 GTA10579: Travel Award - Andrew Robson APRES Conference, project development with University of Florida, USDA and Birdsong Peanut GRDC National Region Contract 02/07/2013 Start Contract End Contact . Helen Kamel Organisation Phone 12/07/2013 Project Code GTA10579 Department of Agriculture, Fisheries and Forestry 746315380 Email [email protected] Project Summary 6. Building Skills & Capacity The sheer scale of the American Peanut industry offers far more opportunity for rapid resourcing and development of the prototype remote sensing and GIS applications. This support will allow for a greater opportunity to develop a commercially adoptable process that will improve yield forecasting, crop variability mapping, paddock traceability and crop/variety auditing for protection if 136 intellectual property rights. This collobration will ultimately result in the faster development of a commercially adoptable product that will not only be relevan to Autstralian Peanut industry but also to the wider Australian grains industry. GTA10592: Travel Award - Huajian Liu 2013 Society for Engineering in Agricuture Conference Contract Start 21/01/2013 Contract End Contact Dr. Chris Saunders Organisation Phone 26/01/2013 Project Code GTA10592 University of South Australia 08 8302 3664 Email [email protected] Project Summary The developed machine vision system can make weed map which presents classified weed batches, distribution and density information which can guide subsequent weed control plans or decision making with a more effective chemical but at reduced overall chemical load on the environment. 6. Building Skills & Capacity GRDC National Region 137 GTA10600: Travel Award - Keith Alcock Genetically Modified Crops Coexistence Conference, GMCC-13 GRDC National Region Contract Start 10/11/2013 Contract End Contact Mr. Keith Alcock Organisation Phone 16/11/2013 Project Code GTA10600 Alcock Consultancy Services 86293112 Email [email protected] Project Summary 6. Building Skills & Capacity The advent of GM crops has transformed grain production in the Americas from north to south. It is crucial that the technologies for abiotic and biotic stress resistance, quality and other traits are further progressed and that they flow through to new crops (especially wheat and barley). Paramount in my involvement is to identify how Australian grain growers can access the technology in then most timely manner. 138 Europe is still the key obstacle to the breakthough to GM food crops. The history of European Government opposition to GM crop production and imports, at least partly driven by Europe-centred organisations like Greenpeace and Friends of the Earth, has influenced Governments throughout the world to prevent or at least restrict access to GM crops and produce. There are signs of progress, but real success stories are limited to maize in Spain and Portugal and to the widening acceptance of maize and soybean imports from the Americas as stockfeed. The return of the GMCC conference to Europe, after excursions to Melbourne and Vancouver, refocuses on the main area where progress is essential. Consumer acceptance will continue to be the major issue impacting market acceptance of GM production and products, particularly as the technology is extended from feed grains (soybeans, maize) and oilseed crops (canola). Overcoming grower concerns about GM technologies will follow many streams testing to prove the safety and communication to the public of the safety and benefits. The contribution of coexistence frameworks to expanded adoption of GM crops is to ensure that the general public and Governments alike are reassured that this is not technolgy being forced on unwilling consumers, but that choice is preserved. It is to be hoped that communities will eventually choose to accept GM crops as safe and indeed essential to feeding the world - but in the short term coexistence is essential if Australian grain production is to avoid the stalemate and 10+ years delay in GM crop adoption that beleaguers European grain and livestock producers. GTA10605: Travel Award - Dr Megan McDonald 10th International Congress of Plant Pathology 139 GRDC Region National Contract End Contact . Megan McDonald Organisation 01/09/2013 Project Code GTA10605 Australian National University Phone Email Project Summary The International Congress of Plant Pathology 2013 (ICPP13), organized every five years, provides and excellent and rare opportunity to engage with a large number of international scientific experts in disease management and control. The current theme ‘Bio-security, Food Safety and Plant Pathology: The Role of Plant Pathology in a Globalized Economy’ aligns tightly with one of the major goals of the GRDC to secure Australia against emerging or novel grain pests in an expanding international market. A broad range of both fundamental research sessions, including molecular hostpathogen interactions, and applied sessions, such as plant pathology extension, are scheduled. This allows attendees to expand their knowledge and interact with both lab and field experts. Additionally our lab leader has arranged a half day meeting and tour of the facilities at the Luancheng Agro-Ecosystem Experimental Station, hosted by Prof. Shen Yanjun. This facility hosts three-research groups focused on molecular wheat breeding, genetic improvement of wheat germplasm and drought-resistance breeding. This visit offers a great opportunity to establish personal collaboration and communication with Chinese wheat breeders. 6. Building Skills & Capacity Contract 24/08/2013 Start GTA10625: Travel Award – ICC-AGSA 2013 GRDC Region National Contract 24/08/2013 Start Contract End Contact Ms. Anita Needham Organisation Phone 29/08/2013 Project Code GTA10625 University of Wollongong 458850088 Email [email protected] Project Summary BACKGROUND: Sorghum is an antioxidant-rich, gluten free cereal grain that is grown locally and represents a novel high value food ingredient in the manufacture of new grain products for the Australian food sector. Sorghum may confer similar favorable human health benefits as other whole grains, with the added value of being a sustainable crop to grow (on the basis of its high drought tolerance and ability to survive harsh conditions common to Australia). 6. Building Skills & Capacity However, sorghum’s unique nutritional, environmental and economic benefits have not been exploited by farmers, the food industry and consumers in Australia, largely due to its reputation as a livestock feed, the lack of potential roles and markets and the lack of quality human studies investigating effects of consuming sorghum. 140 Therefore, our research team has embarked on building an evidence base for potential health benefits of consuming sorghum (in world first human-centered dietary trials) as well as an important investigation of the feasibility of introducing sorghum-based food products into the Australian human food supply chain, including an assessment of the palatability of sorghum products as an indicator of acceptability in the market place. IMPORTANCE OF ATTENDING ICC-AGCA 2013: The ICC-AGCA 2013 Forum represents an exciting opportunity to open up discussions about sorghum as an important food source for Australians with key stakeholders (grain growers, researchers, consumers, food industry representatives) and to debunk old myths that sorghum is merely a low value dollar animal feed. This will not only be achieved through presenting our findings in an oral/poster presentation but also through the opportunity to develop relationships and collaborations with key stakeholders to enable future access to cutting-edge knowledge. Specifically, the opportunity to meet Professor John Taylor from the University of Pretoria is of great significance to me. Professor Taylor is a world expert in sorghum and is the author of several papers that call for more human focused studies, not unlike the research I have embarked upon in my PhD 6 months ago. Professor Taylor’s expansive understanding of sorghum will give me new knowledge that will help develop my studies and ultimately contribute to the evidence base required to provide a step-change towards acceptance of sorghum food products by consumers. Such multi-stakeholder collaborations and sharing of knowledge will help to drive changes necessary to bring sorghum products into a competitive marketplace and help them succeed. BENEFITS TO GRAINS INDUSTRY: Currently sorghum is Australia’s third largest crop but is used mainly for animal feed/pet food. Understanding the potential roles and alternative markets for sorghum beyond this is an important starting point in terms of increasing production targets (human food consumption, household building materials, ethanol). This is particularly important in the face of climate change - sorghum is more environmentally sustainable and cheaper to grow compared to other major cereal crops. Therefore education and research are critical in developing the ‘science push’ for sorghum as a health promoting food - this knowledge needs to be interpreted and disseminated to the Australian grains industry (farmers, producers), food products developers, marketers and key government organisations (i.e. GRDC), dietitians/nutritionists and importantly to consumers. Ultimately, this will give the Grains Industry a competitive advantage as sorghum production increases to meet demands for novel grain products (breakfast cereals, pasta, snacks, breads, bakery goods) that are inherently ‘functional’ - nourishing, rich in antioxidants, slowly digested and gluten-free. This is very important given the consumer ‘pull’ for novel functional foods. As it stands, the grains and food industry in general are overlooking a healthy, sustainable and cost-effective food source that could be profitable for industry and accepted by consumers. 141 6. Building Skills & Capacity GTA10638: Travel Award - Scott Boden Attendence of International Wheat Genetics Symposium GRDC National Region Contract Start 07/09/2013 Contract End Contact Prof. Steve Swain Organisation Phone 18/09/2013 Project Code GTA10638 CSIRO Plant Industry 62465468 Email [email protected] Project Summary By obtaining up-to-date information about the international capabilities of wheat genetics, including available sequence information and tools being developed to improve functional characterisation of genes in wheat, I expect to improve the outcomes of my project which are designed to increase yield potential in wheat through modifying the amount of seed produced on a wheat spike. 6. Building Skills & Capacity A significant aim of attending the conference will be to meet and develop international collaborations with key note speakers who have strong research backgrounds in wheat functional genomics, to ensure that future aspects of my career in wheat genetics are in 142 alignment with the cutting-edge capabilities of wheat research. By visiting the lab of Junko Kyozuka, a world-leader in rice inflorescence genetics, I would like to establish a collaboration to foster the development of wheat inflorescence genetics, which is currently lagging behind similar research in rice and maize. It is anticipated that development of such research in wheat will lead to improvements in seed number per inflorescence, as such studies are showing that modifications in inflorescence architecture have been vital to yield improvements in rice and maize. GRDC National Region Contract 08/09/2013 Start Contract End Contact Dr. Alice Lee Organisation Phone 12/09/2013 Project Code GTA10643 University Of New South Wales 02 9385 4363 Email [email protected] Project Summary Development of linkages with international scientists, esp. those working in antioxidants and functional foods, novel processing, and analytical technology, which will help our research group (at UNSW and collaborators) and the Australian peanut industry to stay upto-date with the latest research developments. In particular, I will be exploring techniques being used in other crops to rapidly and cheaply screen genotypes for functional food traits. This is particularly relevant in functional food research, as techniques are transferrable and hence the peanut industry can learn from what is being done in other crops. 143 6. Building Skills & Capacity GTA10643: Travel Award - Kim-Yen Phan Thien To participate in the 13th ASEAN Food Conference 2013 on Meeting Future Food Demands: Security and Sustainability GTA10705: Travel Award - Ehsan Tavakkoli (UA) 20th World Congress of Soil Science (WCSS) GRDC National Region Contract Start 06/08/2014 Contract End Contact . Chelsea Dubois 13/06/2014 Project Code GTA10705 Organisation Phone 83133716 Email [email protected] Project Summary 6. Building Skills & Capacity Productive, healthy soil is the foundation of Australia’s sustainable grain and food production. The foundation of this success is soil quality, yet grain producers have to cope with some of the poorest agricultural soils in the world. Soil organic matter is the driver of many chemical and biological processes that underpin agricultural production and which influence the efficient use of water and nutrients. 144 Surprisinlgly, given the importance of producitve soils to the grains industry both in Australia and internationally, there is still much we don’t know about soil-plant intercations in alkaline soils and this limits our ability to develop mangement strategies and to understand the key physiological processes that may improve crop adaptation. The major aim of this conference is to bring together international scientists to review our current state of knowledge of soil-plant interactions, identify important research gaps and to suggest the most productive areas for future work. This will allow important collaborative links to be developed as well as exposing Australian based research to a wider audience. The conference, whose theme is, ?Soils Embrace Life and Universe,? will consider a wide range of scientific programs and renowned experts will address the pertinent issues and technological advances in the broad areas of soil science. An objective of my current research activities is to develop a research capacity focussed on C and nutrient cycling in soils within the School of Agriculture, Food and Wine at the University of Adelaide. I believe that a South Australian research hub focussing on research in this field is essential given that concerns over food security and climate change will undoubtedly continue to grow in the future. Attending the 20WCSS will be an excellent opportunity to partially achieve this goal through consolidation of existing collaborations and development of new collaborations with leading research groups who will also attend this conference. GTA10706: Travel Award - Elizabeth Kingston (WNRM) Resilience 2014 145 GRDC Region National Contract End Contact . Tyanna Jankauskas 14/05/2014 Project Code GTA10706 Organisation Phone 96703100 Email [email protected] Project Summary The social, economic and environmental issues of the Wheatbelt community, industry and natural landscape will be presented to an international audience of research scientists and industry stakeholders. The future resilience of the WA Wheatbelt industry will be discussed during this presentation and debated by the audience. The future pressures on the WA Wheatbelt industry and environment, such as market price, government policies and climate variability issues will be presented to international experts from the perspective of a natural resource management NGO operating in this grain-growing region. By attending this international conference it is hoped that international research interest will be attracted to the grain growing industry and environmental issues of the WA Wheatbelt, which will attract opportunities for international research funding. At the conference, similar case studies from around the world will be sought and any successful resilience strategies will be bought back to the Wheatbelt community. 6. Building Skills & Capacity Contract 28/04/2014 Start GTA10707: Travel Award - Glen Fox (UQ) 23rd Institute of Brewing & Distilling Convention - Asia Pacific Section (ht GRDC National Region Contract Start 23/03/2014 Contract End Contact . Melissa Glendenning 29/03/2014 Project Code GTA10707 Organisation Phone 733460550 Email [email protected] Project Summary Hearing the latest research findings on processing and new technologies for barley, malting and brewing will have benefit for Australian barley breeding companies, malting and brewing industries, through the development of research projects to keep improving the quality of Australian malting barley. 6. Building Skills & Capacity This information will have flow to growers 146 through the continued development of high quality malting varieties for the domestics and export markets. As mentioned previously, the expanded network will provide the opportunities for international collaboration for post-graduate students (capacity building), research and new method development GTA10709: Travel Award - Jason Terpolilli (UMU) The three objectives of this study tour are:1. Present my research paper at th Contract Start 09/05/2014 Contract End Contact . Kellie O'Toole 18/09/2014 Project Code GTA10709 Organisation Phone 893602557 Email [email protected] Project Summary My research is targeted at improving nitrogen fixation in grain legumes such as pea, bean and chickpea by understanding the metabolic dialogue between nitrogen fixing rhizobia and their legume hosts. We have made an important start in developing a new model to explain how the host plant powers this process in peas, but we need to build additional collaborative links worldwide and acquire more RNB germplasm to broaden this model to a wider range of symbiotic associations. Ultimately, this metabolic information will enable us to better select elite RNB to overcome suboptimal nitrogen fixation and to boost N-inputs by legumes in agriculture. 6. Building Skills & Capacity GRDC National Region 147 GTA10712: Travel Award - Julien Bonneau (UM) PAG GRDC Region Contract 01/10/2014 Start Contract End Contact . Cassie Watts 16/01/2014 Project Code GTA10712 Organisation Phone 383445066 Email [email protected] Project Summary The Australian grains industry is highlighted in this presentation through use of Australian cultivars (RAC875, Gladius, Excalibur, Kukri, and Drysdale) where RAC875, Excalibur and Drysdale provide better tolerance to heat and/or drought across a wide range of environmental conditions at the national level (SA and NSW) and international level (Mexico - CIMMYT). An international collaboration with INRA (France) was extremely valuable for this project, giving access to chromsome 3B sequencing data including gene annotation. 6. Building Skills & Capacity The talk will be presented to a large international audience which could foster new collaborations, possibilities to exchange plant material, and provide access to additional wheat production sites where abiotic stresses 148 (i.e. high temperature and water deficit) occur throughout the growth cycle. This work also makes significant contributions to grain crop genetics through the discovery of candidate gene(s) for yield improvement under abiotic stress (heat and drought). The major outcome of this travel award is the ability to disseminate molecular marker information which is of great value to Australian wheat breeders and therefore to the Australian Grain Industry in general. The results could be incorporated into molecular marker assisted selection schemes and implemented directly into breeding programs. GTA10715: Travel Award - Manuel Zander (UQ) International Plant & Animal Genome XXII Conference Contract Start 01/09/2014 Contract End Contact . Kaye Hunt 22/01/2014 Project Code GTA10715 Organisation Phone 61733653458 Email [email protected] Project Summary By presenting my work at the PAG conference, I will have the chance to represent the University of Queensland and the Australian grains industry to a worldwide audience, thus enhancing our exposure globally. Attending the conference will also give me the opportunity to meet fellow peers in my field of research and learn to better understand the issues and challenges that we face in solving scientific problems. The meetings will also allow me to arrange and finalise visits to local research institutions during the days following the completion of the PAG conference. Hearing the experts in my field present their work will be a good opportunity to capture the current state of the Blackleg genetics research worldwide as the major researchers from France and Canada will be in attendance. The Australian research community is a small fraction of the worldwide scientific establishment, and gaining the chance to communicate with members from all around the globe would be a great experience, not only to my research but also on a personal and professional level. In this way collaborations can be formed between my current research group in Brisbane and the leading researchers in plant-fungal interactions from around the world, allowing us to share invaluable insights and experience. This will ultimately lead to better research outcomes that will benefit the Canola industry in Australian and ensure that the blackleg fungal disease is kept under control. The better we can learn to understand the relationship between Canola and the Blackleg fungus, the greater the chance of developing long-term and stable solutions for the Australian industry. 6. Building Skills & Capacity GRDC National Region 149 GTA10717: The current status, potential applications and future directions of bacterial biocontrol research against Sclerotinia sclerotiorum of canola in Australia GRDC Region Contract 24/11/2013 Start Contract End Contact . Kamala Anggamuthu 29/11/2013 Project Code GTA10717 Organisation Phone 269332420 Email [email protected] Project Summary 6. Building Skills & Capacity Sclerotinia stem rot of canola is becoming increasingly important in south-eastern Australia. Until recently, Sclerotinia epidemics were thought to occur once in a five to eight year period and an increase inoculum pressure was occurred last year in high rainfall zones of NSW. 150 In surveys conducted in 1998, 1999 and 2000, as part of a GRDC funded project at Charles Sturt University, levels of stem rot in some crops were found to exceed 30% in all years. This could correlate to yield losses of 15 to 30%. Estimates of losses due to Sclerotinia in 1999 in New South Wales alone exceeded $170 million. As there is no resistance currently available in Australian canola varieties, management of the disease solely relies on the strategic use of fungicides combined with other cultural management practices. Reduced performance of fungicides and environmental concerns has lead to search for an alternative bio-control strategy against Sclerotinia sclerotiorum in canola. Efficient knowledge regarding the biological control of Sclerotinia sclerotiorum through the use of an antagonistic bacterial agent is still scarce in comparison to fungal parasites in canola. Currently there are no bacterial biological control agents commercially available for control of Sclerotinia diseases in Australia. The present investigation will explore the current status, potential applications and future directions of bacterial biocontrol research against Sclerotinia sclerotiorum of canola in Australia. GRDC National Region Contract 01/01/2014 Start Contract End Contact . Pandy du Preez 15/04/2014 Project Code GTA10718 Organisation Phone 864883863 Email [email protected] Project Summary Terminal drought is a major problem in the Australian grains industry. This proposed visit is the extension of my PhD project, where I am working on a wild X domesticated recombinant inbred line (RIL) population to explore the physiological response of yellow lupin to terminal drought and early phenology. Lupins generally face terminal drought which significantly affects their grain yield. So far, breeders have only focused on yield and developed cultivars with early, highly temperature-responsive phenology that escapes drought in their warm, short-season target environments. Thus the early flowering crops can complete grain filling in favourable conditions but this has been achieved on the expense of vegetative growth which results in fewer potential flowering and podding nodes. In previous studies, the more widely cultivated narrow-leafed lupin showed no genetic variation for drought stress response except its ability to survive terminal drought by following drought escape strategy (early phenology). In this study, we are testing the hypothesis that yellow lupin has variation for response to drought stress which is not explained by drought escape. We are using a yellow lupin RIL population which was developed from a cross between ‘Wodjil’ (cultivar of low rainfall origin) and P28213 (wild type of high rainfall origin). Because of the diverse evolutionary background from low and high rainfall origins, this RIL population is valuable for identifying genetic factors mediating response to drought stress. The information obtained will help plant physiologists better understand lupin's behaviour under drought stress and will enable plant breeders to adopt efficient crop improvement approaches to develop a welladapted crop. The RIL population is currently being phenotyped for drought in a replicated field trial where it is segregating for phenology and drought adaptation traits. This phenotypic information will be correlated by genomic information obtained during my visit to the lab of Prof Joshua Udall at the Genetics and Biotechnology lab, Brigham Young University (BYU), Provo, Utah, USA. Prof Udall played a key role in sequencing lupin transcriptomes and SNP discovery in yellow lupin. I would use next generation DNA sequencing technology to sequence so-called ?RAD-tag? genomic libraries for the genotyping of my experimental RIL population. Ultimately, this genomic study will help in the development of a comprehensive genetic map of yellow lupin for mapping drought and phenological adaptation quantitative trait loci (QTLs). The resultant linkage map and QTLs will serve as an important genetic resource for the germplasm conservation, core collection characterization and for marker-assisted selection (MAS) in future breeding programmes. These resources will help breeders working in Australian lupin industry to utilise this information in their breeding programmes for devising efficient crop improvement 151 6. Building Skills & Capacity GTA10718: Travel Award - Muhammad Munir Iqbal (UWA) To present poster in Plant and Animal Genome (PAG) Conference, 2014 and study vi approaches to develop a well-adapted sustainable lupin crop. This visit will help promote collaboration between Australian and USA researchers with complementary research strengths working in lupin genomics. 6. Building Skills & Capacity The scientific publications arising from this 152 collaborative research will reach more prestigious journals than would otherwise be possible. This will also help in raising the profile of Australian agricultural science research. GTA10721: Travel Award - Enli Wang, Zhongkui Luo, Hongtao Xing (CSO) 20th World Congress of Soil Science Contract Start 01/06/2014 Contract End Contact . Minka Schwabe 15/01/2014 Project Code GTA10721 Organisation Phone 262464046 Email [email protected] Project Summary 1. Better undertanding and prediction of soil carbon sequestration potential and its impacts in Australian cropland 2. Gain frontier knowledge of soil carbon and nitrogen research, contributing to development of sustainable and productive cropping and management systems in Australia 3. Knowledge gained directly contributes to national soil carbon research funded under the Filling Research Gap program 6. Building Skills & Capacity GRDC National Region 153 GTA10786: Travel Award - Shi Ying Yang (UA) Attend the Plant Genomics Congress:Asia GRDC Region National Contract 23/02/2014 Start Contract End Contact . Chelsea DuBois 27/02/2014 Project Code GTA10786 Organisation Phone 883133716 Email [email protected] Project Summary 6. Building Skills & Capacity I will promote our Austrlian plant research on the genomic research areas, and develop relevant national and international 154 collaboration proposal with attendees. ICN00017: Industry Pertnership Initiative 155 GRDC Region Contract End Contact Project Code Organisation Phone Project Summary 31/03/2014 Email ICN00017 6. Building Skills & Capacity Contract 28/11/2013 Start IDA10355: Industry Development Award Living Farming - Farming systems and the continued problem of herbicide resistance GRDC National Region Contract Start 30/06/2012 Contract End Contact Mr. Richard Devlin Organisation Phone 03/09/2012 Project Code IDA10355 Living Farm 08 96412845 Email [email protected] Project Summary Wheat is generally regarded as the most profitable crop on Western Australian farms, and with Western Australia currently producing approximately 36% of the Australian wheat crop it is also of significant economic importance to Australia as a whole. 6. Building Skills & Capacity Herbicides are the primary tool for weed control in this production system but their sustainability is susceptible to the evolution of herbicide resistant weed populations 156 throughout Australia. By allowing Western Australian growers to visit leading industry bodies in South Australia, further knowledge can be obtained regarding the importance of monitoring herbicide use and slowing the development and spread of herbicide resistance, which is rapidly becoming one of the major constraints to crops throughout grain production areas of Australia IDA10441: Attendance & dissemination of info from the Global Herbicide Resistance Conference Feb 2013 Contract Start 20/02/2013 Contract End Contact Mrs. Erin Green Organisation Phone 22/02/2013 Project Code IDA10441 Yuna Farm Improvement Group 0899205454 Email [email protected] Project Summary This proposal is relevant to the Australian grains industry because the Yuna area contributes in excess of 200,000 tonnes of grain into the economy annually. If we can improve our weed control it will result in greater yields, less costs and a more sustainable future for our members and the wider grains industry. Due to our reliance on continuous cropping we have been dealing with resistance issues for 20 years. If we can show other areas in Australia the impact that resistance can have and ways to manage it, we can help other growers be proactive in preventing and managing resistance. Due to YFIG's participation in the WA Grower Group Alliance (GGA) and North East Farming Futures (NEFF) we are able to easily share information with growers throughout the state. 6. Building Skills & Capacity GRDC National Region 157 IDA10447: Industry Development Award AgriKnowHow - Precision Agriculture (PA) and Information and Communication Technology (ICT) study tour GRDC National Region Contract 15/06/2013 Start Contract End Contact Ms. Emma Leonard Organisation AgriknowHow Phone 08 8834 1233 01/08/2013 Project Code Email IDA10447 [email protected] Project Summary PA pays - of those who have adopted PA 72% reported an ecconomic benfit (PA Grower groups survey). Yet, in Australia, PA uptake has focused on a few applications. 6. Building Skills & Capacity There are opportunities across the produciton chain, including disease and pest managment and automated equipment. ICT offers hugh benefits to our small and dispersed population, 158 including the rapid delivery of information as well as remote management. To meet the increasing costs of production, reducing terms of trade and increasing global food demand, we need to seek out these technologies, so they can be tested and applied in Australia. GRDC National Region Contract 01/01/2013 Start Contract End Contact Mr. Leighton Pearce Organisation Phone 02/01/2013 Project Code IDA10452 Dodgshun Medlin Agricultural Management Pty Ltd 1300 329888 Email [email protected] Project Summary Many grain growers are reviewing their current seeding machinery due to federal government rebate through the clean energy future program. Dodgshun Medlin see an opportunity to coordinate a celebration of the regions progress in relation to No-till farming, along with demonstrating the next phase of conservation farming in the region. 159 6. Building Skills & Capacity IDA10452: Industry Development Award Dodgshun Medlin Agricultural Management Ten Years of No-till in the Victorian Mallee "Celebration and Demonstration" IDA10463: Industry Development Award - DAN Understanding and interpreting grains research and extension GRDC National Region Contract Start 20/06/2013 Contract End Contact Mr. Rohan Brill Organisation Phone 23/06/2013 Project Code IDA10463 NSW Department of Industry and Investment 0488 250 489 Email [email protected] Project Summary 6. Building Skills & Capacity The project will advance the uptake of research outcomes across farms in the northwest and central-west plains regions. The project will give participants the skills to interpret and question information supplied to 160 them in all forms, from scientific publications to product brochures promoting new products as well as being able to question farm advisor recommendations. IDA10482: Industry Development Award Riverine Plains - Resistance Roadtrip 161 GRDC Region National Contract End Contact Ms. Fiona Hart Organisation Phone 26/02/2013 Project Code IDA10482 Riverine Plains Inc 03 5744 1713 Email [email protected] Project Summary The RPI members survey showed that 99% of respondants were using no-till, min-till, direct sowing or reduced till systems. All these systems have a heavy dependency on herbicides for weed control and managing resistance is a constant challenge to farmers. Resistance to groups A & B in ryegrass is widespread, with resistance to Treflan and Glyphosate beginning to be recorded. Diversity of management is key to managing herbicide resistance and this study tour provides a unique opportunity to combine learning from international and Australian research scientist’s with learning from farmers that have developed and are using integrated weed management techniques to effectively manage weed populations on their properties. 6. Building Skills & Capacity Contract 20/02/2013 Start IDA10503: Industry Development Award Morowa Farm Improvement Group - Global Herbicide Resistance Challenge Conference GRDC National Region Contract Start 20/02/2013 Contract End Contact Mr. Mark Coaker Organisation Phone 23/02/2013 Project Code IDA10503 Morawa Farm Improvement Group Inc 08 9971 6087 Email [email protected] Project Summary 6. Building Skills & Capacity The conference will cover information regarding weed resistance. Will include information about world best practice and 162 worldwide herbicide resitance issues. IDA10508: Industry Development Award Holmes Farm Consulting - Pulse market and agronomic opportunities Contract Start 06/01/2013 Contract End Contact Mr. Sam Holmes Organisation Phone 30/06/2013 Project Code IDA10508 Holmes Farm Consulting 888322043 Email [email protected] Project Summary The Australian grain growers require more informed market access information. By allowing growers to meet with end users directly and to experience the different culture they are growing pulses for will provide relevant information about the varieties grown, particularly lentils. The previous trip conducted by PBA to end users was conducted when there was a shortage in the market, however presently there is low demand for lentils allowing a more timely assessment of the varieties grown and soon to be released. It will also provide the Australian grain industry with information on how Canadian growers manage pulses and the disease and quality issues they deal with along with recent research activities and how it can be applied for farmers in Australia. 6. Building Skills & Capacity GRDC National Region 163 IDA10513: Industry Development Award Hooper Consulting - North America farmers study tour GRDC National Region Contract Start 07/07/2013 Contract End Contact Mr. Peter Hooper Organisation Phone 20/07/2013 Project Code IDA10513 Hooper Consulting 0427 225 590 Email [email protected] Project Summary 6. Building Skills & Capacity There is great value for farmers to spend time outside of their own businesses and look at how other farmers and business people are approaching their own set of problems and the technologies that they are using to do so. With the tour group comprising approximately 25 young and energetic farmers that will form the future of their local farming communities, it is important that they are encouraged and stimulated to look for the next steps that their 164 businesses can take with the best knowledge of new innovative tools that are available. This tour will provide a great opportunity for these farmers to learn more about new and innovative agronomy, technologies and business concepts that they can apply in their own businesses. GRDC Region National Contract 06/07/2013 Start Contract End Contact Mrs. Debby Clarke Organisation Phone 14/07/2013 Project Code IDA10587 WA Grains Group Inc 898654041 Email [email protected] Project Summary In addition to WAGG’s marketing interests a number of important industry drivers will be agenda items during our China meetings. Content of and outcomes from these discussions will be of significant interest to the Australian Grains industry, including: 1. Research into farm storage of grain in Nitrogen at the Academy of Grain Science China (a project WAGG members Doug and Debby Clarke are long term collaborators on), 2. encouraging patient capital from the Beidehuang Group and/or COFCO, and 3. research into cultivar development and grain quality undertaken by the Australian-China Centre for Wheat Improvement group (Murdoch University/Chinese Academy of Agricultural Science). 165 6. Building Skills & Capacity IDA10587: Industry Development Award WAGG in China - Continuous improvement and innovation of grower education and market development within the complex agricultural relationships between China and Western Australia. IDA10588: Industry Development Award Mallee Sustainable Farming Karoonda Field Day 2013 GRDC National Region Contract Start 01/09/2013 Contract End Contact Ms. Donna Robertson Organisation Phone 02/09/2013 Project Code IDA10588 Mallee Sustainable Farming Project Inc 350219100 Email [email protected] Project Summary Weed management, paricularly in the Mallee regions of southern Australia is an important topic, and goes hand-in-hand with other issues such as water use efficiency and herbicide resistance. 6. Building Skills & Capacity By incorporating microwave technology for weed management, this will contribute greatly to the grains industry in terms of increasing 166 efficiency and profitibility of Mallee farmers. With an ever-increasing average age of Mallee farmer, health is a topic that hits close to home and ways in which farmers can make small changes to look after themselves can benefit the industry. IDA10589: Industry Development Award South Australian grain production with declining rainfall tour Contract Start 13/10/2013 Contract End Contact Mr. Gerald Williams Organisation Phone 18/10/2013 Project Code IDA10589 Corrigin Farm Improvement Group Inc. 0487 321 067 Email [email protected] Project Summary To ensure that the local area has a viable grain production future it is imperative that we review and adopt from the lessons that have been learnt in other areas. Central parts of the Western Australia wheatbelt, in particular, are being challenge by decreasing winter rainfall and increased frost incidence due to the change in weather patterns. This is perhaps the greatest challenge many farmers in the area have faced in their farming career. Historically this has been a reliable grain growing area but now many farmers and now facing yield decline. In order to stop this decline Corrigin farmers are keen to review and improve their farming systems to adapt to the changing conditions at hand. This could provide a good example to other farmers in other areas, if better efficiency can be achieved. Corrigin Farm Improvement Group is a strong farming group with a history of contributing onfarm research and information back to the farming community. 6. Building Skills & Capacity GRDC National Region 167 IDA10596: Industry Development Award Learning the best from the West - FarmLink WA Study Tour GRDC National Region Contract Start 04/08/2013 Contract End Contact Miss. Judith Neill Organisation Phone 16/08/2013 Project Code IDA10596 FarmLink Research Pty Ltd 269244633 Email [email protected] Project Summary Learning and adopting new management practices from other farmers who have a deeper experience of emerging problems in our regional grains industry has proven to be a very successful extension activity for southern NSW farmers. 6. Building Skills & Capacity Issues of concern for local growers to be 168 studied include herbicide resistance management, machinery development, managing changes to farm scale, innovative machinery and farm ownership/leasing models and climate change adaptation. IDA10601: Industry Development Award - The Changing Nature of Agricultural Training 169 GRDC Region National Contract End Contact Ms. Nickie Berrisford Organisation Phone 07/08/2013 Project Code IDA10601 Partners in Grain Inc. 352264075 Email [email protected] Project Summary Female partners in the farm business have a strong influence on farm practices and adoption of improved practices. In general partners have greater separation from day to day farm operations resulting in a more questioning and holistic view of the farm business and current practices. This tour exposes key women to industry research, innovation, processes and practices to increase women’s involvement in the industry at the farm level and beyond. The proposal also allows for participants to think about how to apply what they have learnt to their business and farming system group once they return to their state.. 6. Building Skills & Capacity Contract 04/08/2013 Start IDA10614: Industry Development Award Farming systems and the move to continuous cropping GRDC National Region Contract Start 02/09/2013 Contract End Contact Ms. Rebecca Jenkinson Organisation Living Farm Phone 896412845 07/09/2013 Project Code Email IDA10614 [email protected] Project Summary Wheat is generally regarded as the most profitable crop on Western Australian farms, and with WA currently producing about 36% of the Australian wheat crop it is also of significant economic importance to Australia as a whole. 6. Building Skills & Capacity Growers in WA wish to increase the number of years they can continuously crop their paddocks, increasing wheat production, while also adhering to sustainable agricultural practices. However, soil compaction constraints are impeding this ability. The cost 170 of lost crop and pasture production from soil compaction is currently estimated at $333 million (DAFWA, 2011) in cropping regions of WA. By allowing WA growers to visit leading industry bodies in Queensland, knowledge can be obtained regarding the importance of soil health and functions of slowing the development of compaction in continuous cropping programs. IDA10621: Industry Development Award Grower attendance - Australian Summer Grains Conference June 2013 Contract Start 16/06/2013 Contract End Contact Mrs. Jennie Hehir Organisation Phone 20/06/2013 Project Code IDA10621 The Maize Association of Australia 358834445 Email [email protected] Project Summary As a representative of maize growers and as a summer grain grower myself I see the requirement for growers to be educated about grower requirements to meet the ever demanding need from exporters. It is obvious on ground the there is massive potential for maize production to esculate in the future. In having said that we must ensure growers understand the stringent requirements when exporting their produce. It will only take one shipment of inferior grain to be exported to deter these esculating market opportunities. 6. Building Skills & Capacity GRDC National Region 171 IDA10728: RPI New Zealand Study Tour GRDC Region Contract 13/01/2014 Start Contract End Contact Mrs. Fiona Hart Organisation Phone 19/01/2014 Project Code IDA10728 Riverine Plains Inc 57441713 Email [email protected] Project Summary 6. Building Skills & Capacity This proposal is relevant to the Australian grains industry as participants will gain valuable knowledge of New Zealand crop production systems with an emphasis on high yeilding, high input cropping similar to the Riverine Plains. Lessons learnt will aid to assist growers in Australia, particularly the Riverine Plains aim to increase production and 172 profitability through the adoption of new technology/research findings. This study tour will also foster agricultural networks between growers, advisors and researchers in Australia and New Zealand. IDA10732: IDA - WANTFA - Growers South America Study Tour 173 GRDC Region National Contract End Contact Miss. Lauren Celenza Organisation Phone 09/01/2014 Project Code IDA10732 WA No-tillage Farmers Association 419908793 Email [email protected] Project Summary Growers are our most practical researchers, and without them being committed, stimulated and successful in the work that they do, we have no grains industry. This study tour will bring together well-connected, discerning growers to take a global persective about challenges and opportunites they face, and explore novel ideas. Tour participants will get first-hand exposure to different production systems, RD&E models, market influences, public perception of agriculture, and roles of government and industry. This will highlight where Australia sits on the world scale as well as indentifying practical activities that might be trialled or incorporated to improve current Australian practices. WANTFA members have strong connections with other industry and grower groups, and there will be informal and formal dissemination of their learnings through presentations, conversations and other activities they pursue as a result of what they have seen overseas. 6. Building Skills & Capacity Contract 08/01/2014 Start IDA10734: IDA - RAIN - Perth 2014 Agribusiness Crop Updates GRDC Region National Contract 23/02/2014 Start Contract End Contact Miss. Elisa Spengler 26/02/2014 Project Code IDA10734 Organisation Phone 898381018 Email [email protected] Project Summary 6. Building Skills & Capacity Growers who attend this event can adopt their new learnings on farm. They can also share these learnings with their grower groups, community, neighbours and collegues - 174 resulting in greater adoption of research and development outcomes. IDA10736: IDA - MSF - Mallee Sustainable Farming team participating in the GRDC Update for Advisors - South Australia Contract Start 25/02/2014 Contract End Contact Ms. Donna Robertson Organisation Phone 26/02/2014 Project Code IDA10736 Mallee Sustainable Farming Project Inc 350219100 Email [email protected] Project Summary The grains industry will benefit from the study tour for the following reasons: men and women are inspired and motivated to improve the way in which they operate within the mallee to help farmers to increase skills and knowledge of building successful farm businesses; networking opportunity for everyone in the industry; and linkages to growers, marketers, researchers and media are fostered during the event. 6. Building Skills & Capacity GRDC National Region 175 IDA10740: IDA - ASS - Study tour of research and farming practices that improve soil management in the low rainfall Mallee GRDC National Region Contract Start 07/01/2014 Contract End Contact Mr. Sam North 15/08/2014 Project Code IDA10740 Organisation Phone 58819926 Email [email protected] Project Summary The focus of the study tour is to see how farming systems in the Mallee have changed over the past 15 years to increase productivity while improving soil health. Soil is the key asset that drives the productivity and sustainable soil management practices are important for productive and profitable grains industry in the future. 6. Building Skills & Capacity Research in the Mallee has focused on how 176 no-till, stubble retention and intensive cropping can improve the soils biological health and prevent soil degradation through erosion. Mallee farmers are also industry leaders at soil specific management with precision agriculture. Therefore there are many leading farming practices in the Mallee that could be applicable to Riverina farming systems. IDA10741: IDA - Rural Directions - Study Tour to WA Northern Wheatbelt to look at Herbicide Resistance and Other Issues Contract Start 14/07/2014 Contract End Contact Mrs. Sarah Heinjus 18/07/2014 Project Code IDA10741 Organisation Phone 888414500 Email [email protected] Project Summary The grains industry is putting a lot of resources into integrated weed management, and this tour will highlight the extreme impacts of resistant radish. In many ways the WA experience with herbicide resistant radish is a useful case study of resistance in a broadleaf weed. Using this to educate other growers is a way of avoiding repeating the same pattern. Aside from herbicide resistance, the WA grainbelt has other features that provide lessons for SA growers. These include managing large scale cropping enterprises, amelioration of acidity and non wetting sands, and growing and professionalising the family farm. 6. Building Skills & Capacity GRDC National Region 177 IDA10743: IDA - Ag Excellence Alliance Annual Grower Group Forum GRDC Region National Contract 17/03/2014 Start Contract End Contact Mrs. Mandy Pearce Organisation Phone 18/03/2014 Project Code IDA10743 Ag Excellence Alliance Inc 418805670 Email [email protected] Project Summary 6. Building Skills & Capacity The Ag Ex forum targets those at the operational level of grains industry RD&E in SA. This is the only forum where farming groups from across the state can network, hear each others stories and learn new skills. Group members are able to meet each other face to face and discuss issues of common concern and to explore opportunities to work more collaboratively. 178 The forum provides an effective mechanism for policy makers and leaders from key government departments and industry to learn about issues directly from those involved from across the state. Formal presentations allow farmer groups to inform their peers on their achievements and issues, and report on the outcomes of projects. IDA10772: IDA - UNFS - Eastern Low Rainfall Zone Bus Tour 179 GRDC Region National Contract End Contact Mrs. Ruth Sommerville 08/08/2014 Project Code IDA10772 Organisation Phone Email [email protected] Project Summary This proposal aims to explore the climatic and industry challenges faced by farmers in the low rainfall zones of SA, Vic and NSW through visiting trial sites, on farm visits and discussion sessions with the coordinators of the Mallee and Central West Farming Systems Groups. It will look at pest and weed management, modification and adaption of machinery to overcome regional issues, application of precision agriculture and the ways in which Farming System Groups can improve the viability and sustainability of the regions farmers on the small and large scale. 6. Building Skills & Capacity Contract 08/04/2014 Start IDA10784: IDA - BCG - Grower study tour of WA Northern Agricultural Region focused on management of resistant wild radish GRDC National Region Contract Start 08/11/2014 Contract End Contact Ms. Justine Severin Organisation Phone 15/08/2014 Project Code IDA10784 Birchip Cropping Group -354922787 Email [email protected] Project Summary 6. Building Skills & Capacity Herbicide resistance poses a very real threat to the productivity of Wimmera and Victorian Mallee farmland. In the Western Australia Northern Agricultural Region herbicide resistant wild radish has been extremely detrimental to the productivity and profitability of grain growing enterprises, and the lessons learned there should be understood and heeded to avoid a similar situation developing 180 in GRDC's southern region. Allowing key Victorian growers, who are already suffering the early effects of herbicide resistant wild radish, to witness first-hand the consequences of its spread in WA is a very powerful way to motivate practice change and ensure the sustainability of the industry. IDA10798: IDA- RAIN - 2014 Innovation Generation Conference - Toowoomba, Queensland 181 Contract Start 07/04/2014 Contract End Contact Miss. Elisa Spengler Organisation Phone 07/11/2014 Project Code IDA10798 Ravensthorpe Agricultural Initiative Network Inc (RAIN) 898381018 Email [email protected] Project Summary Innovation Generation quotes it is 'the leading annual muster for young people working in Australian agriculture.' The 2013 conference had a theme of 'Australia in the Asian Century' - the 2014 theme is yet to be announced. Youth numbers in agriculture are lessening - it is important to create opportunity for our younger collegues and to challenge how they think. It is also important for them to network with industry and researchers and to see how other farming enterprises operate. 6. Building Skills & Capacity GRDC National Region IDA10799: IDA - Liebe Group - Innovation Generation Young Farmers Tour GRDC Region National Contract 02/01/2014 Start Contract End Contact Mrs. Jemma Counsel Organisation Phone 09/01/2014 Project Code IDA10799 Liebe Group 896610570 Email [email protected] Project Summary 6. Building Skills & Capacity Innovation Generation encourages the development of the future leading farmers of the grains industry. The Liebe Group have a large number of young and lateral thinking farmers, both male and female, who are heavily involved in the group's committees and events, they have a thirst for involvement and 182 building knowledge. This event provides the opportunity to generate new research and development ideas and find solutions to farming issues. LYH00001: Theme 6 Strategy and MERI Plan 183 GRDC Region Contract End Contact Organisation 31/12/2013 Project Code Lyndal Hasselman Phone Project Summary Email LYH00001 6. Building Skills & Capacity Contract 25/10/2013 Start ORM00011: Farm Business Management Updates in the Southern Region GRDC Region National Contract 30/06/2014 Start Southern Region Contract End Contact Mr. Gavin Beever Organisation ORM Pty Ltd Phone 03 54416176 30/06/2015 Project Code Email ORM00011 [email protected] Project Summary 6. Building Skills & Capacity Intensification of cropping, farm expansion, increased debt and an almost doubling of farm costs have been hallmarks of Australian farming over the past decade. This period has also seen some powerful influences of variable weather, for example rainfall (too little, too much at the wrong time) and temperature (frost). Together, these factors have impacted on farm business performance and focussed growers attention on financial risk management and in seeking new and more effective methods and tools to assist them in dealing with the business consequences of these factors. As a consequence, farm decision-making has become increasingly complex as businesses strive to meet the challenges of managing production and financial risks in the face of ever-changing climatic, seasonal and marketing environments. Yet, despite mounting challenges, many farms are prospering. The GRDC Farm Business Updates for Advisers and Growers provides a unique forum to learn from and network with leading growers and industry professionals. The program has a broad range of topics delivered by an outstanding selection of expert speakers. It provides topical and practical advice and information on the key issues facing grain growers and their advisers. This one-year project will: 184 1. Continue the operation of GRDC Farm Business Updates in the GRDC Southern Region (following on from ORM00004 – completion date 30/6/14); 2. Undertake new research and development (R&D) based activities to determine the effectiveness of the Updates, the ongoing needs and improve the content and effectiveness of the Updates. The new R&D activities focus on the following aspects: 1. Evaluate the effectiveness of the Updates delivered as part of ORM00004, including the contribution of the Updates to practice change in advisers and the growers that they support; 2. Develop new resources (Southern Region Gross Margin Guides) to support the Update presenters, with the aim of ensuring content in the Updates is up-to-date and relevant, based on the most recent and accurate farm financial data and provides the best financial tools for advisors to assist growers in optimising their farm business management. Project activities include: Four (4) regionally based GRDC Farm Business Management Updates for Advisers in key regional centres across New South Wales (Wagga), Victoria (Bendigo), South Australia (Adelaide) and Tasmania (Launceston) to present the latest information relating to farm business management. These updates target advisers(agronomists, farm business management consultants, financial service providers and accountants) and others providing fservices to grain Produce four (4) GRDC Farm Business Management Fact Sheets covering new or current topical issues. "Support Farm Business Management Networks’: o Develop and maintain a partnership arrangement with the CPA Australia member bodies in each state to ensure the GRDC Farm Business Updates for Advisers meets CPD requirements and are promoted by CPA Australia; o Grow relationships with and promote GRDC funded organisations who have farm business management related activities, o These include; Nuffield Australia, the Australian Rural Leadership Program, Partners in Grain, Graham Centre and Farming Systems Groups. o Ensure where relevant they are resourced as sources of expert speakers for the farm business updates and are promoted in the proceedings; and o Manage the grdc updates twitter account to promote and extend the reach and impact of the grdc farm business udpates. 185 6. Building Skills & Capacity farming businesses. At each GRDC Farm Business Management Update for Advisers web-stream at least one key topic to extend the reach, engagement and impact of key farm business management topics. Six (6) GRDC Farm Business Management Updates for Growers in regional locations to ensure accessibility to growers across Victoria, New South Wales and South Australia. These one-day updates are to target growers in these regions. Operate and support Update Steering Committees for each State that hosts Update events to ensure that both presentations and materials presented at Updates are accurate, of the highest presentation quality, using credible accountants and other appropriate farm business management expertise. Ensure the content of presentations are tailored and coordinated, consistent and use common farm business management language. Produce six (6) Farm Business Management Update Newsletters targeting advisers (and attendees to Adviser Updates). Produce six (6) pages of content for GRDC Ground Cover covering issues pertaining to the latest information on farm business management. PIG00007: Building the human capacity of grain growers through Partners in Grain GRDC Region National Contract 01/10/2011 Start Contract End Contact Ms. Kim Blenkiron Organisation Phone 30/09/2012 Project Code PIG00007 Partners in Grain Inc. 0427 592 243 Email [email protected] Project Summary This is a 12 month project to develop three program logic's that identify where the opportunities exist to develop regional capacity where it is needed most. Through Partners in Grain Inc. this project will conduct five regional facilitated workshops to determine the training needs in the regions and develop a capacity building program logic and evaluation framework for each of the three GRDC regions. 6. Building Skills & Capacity In addition the project team will deliver a range of regional training activities. High priority targeted workshops will be developed following the regional workshops and twenty five activities will be offered across the 186 regions. This will include five training activities in the West, five in the North and 15 in the South. Thirty e-newsletters will be distributed throughout the regions over the 12 months to promote these and other grains industry training activities. Partners in Grain is a program designed to increase the human resource capital of the Australian grains industry through the facilitation of professional development and networks for all partners in grain businesses. Partners in Grain is grower driven through regional reference groups. SEP00012: Technology integration workshops in the Western Region 187 GRDC Region Contract End Contact Project Code Organisation Phone Project Summary 01/01/2017 Email SEP00012 6. Building Skills & Capacity Contract 01/01/2014 Start TAY00002: GRDC Network Stakeholder Engagement Strategy GRDC Region Contract 13/01/2014 Start Contract End Contact Organisation Project Code Victoria Taylor Phone 6. Building Skills & Capacity Project Summary 188 30/06/2014 Email TAY00002 TEK00001: Western Region Fast TrackDetermining economic rates and incorporation for lime in the Eastern wheatbelt o Contract Start 01/02/2014 Contract End Contact Organisation 28/02/2015 Project Code Tek Ag Phone Project Summary Email TEK00001 6. Building Skills & Capacity GRDC Region 189 UHS10054: Undergraduate Honours Scholarship - Julian Greenwood (ANU) Genetic regulation of cereal spike architecture GRDC National Region Contract Start 01/01/2011 Contract End Contact Dr. Peter Anderson Organisation Phone 09/11/2011 Project Code UHS10054 Flinders University 08 8201 5269 Email [email protected] Project Summary The broad aim of my honours project is to define a novel mechanism that regulates seed size in plants. We understand almost nothing about how plants regulate seed size, more specifically endosperm size. One mechanism influencing endosperm size is genomic imprinting, that is deferentially gene expression of the maternal and paternal alleles. 6. Building Skills & Capacity Until recently only about a dozen of the 25,000 protein encoding genes in the model plant Arabidopsis were demonstrated to be imprinted. Now amazingly 5000 non-coding 190 heterochromatic small interfering RNA (siRNA) producing loci were discovered to be are paternally imprinted. These 5000 loci produce about 100,000 small RNAs! This is a unique and unappreciated level of gene regulation that acts at the posttranscriptional and transcriptional level that I aim to investigate. This project will build on research already in Dr. Searle’s laboratory on the identification of imprinted micro RNAs, another class of non-coding RNAs, and their role in regulating of seed size. GRDC National Region Contract 20/02/2012 Start Contract End Contact . Susan Wilson Organisation Phone 19/01/2013 Project Code UHS10225 University Of New England (02) 6773 2789 Email [email protected] Project Summary Leptosphaeria maculans causes blackleg, the major disease of canola worldwide. Fungal effectors, small secreted proteins are key molecules involved in disease, but so far only five such proteins have been characterized from L.maculans. I will use bioinformatics to seek genes encoding small secreted proteins in the newly acquired genome sequences of L.maculans and in a closely related species, L.biglobosa 'canadensis', which infects canola and junceacanola. I will mutate three of these effectors and test the ability of the resultant fungal strains to cause disease. I will examine when and where these genes are expressed during invasion of canola, which will indicate the roles that these effectors play in disease, and the differences in defence responses of canola to these two closely related fungal pathogens. 191 6. Building Skills & Capacity UHS10225: Undergraduate Honours Scholarship - Bethany Clark (UM) Leptosphaeria effectors: key determinants of blackleg disease of canola UHS10226: Undergraduate Honours Scholarship - Cheryl Day (UWA) Economic impact of soil borne diseases over the entire rotation sequence GRDC National Region Contract 06/02/2012 Start Contract End Contact Prof. Barbara Howlett Organisation Phone 05/02/2013 Project Code UHS10226 University of Melbourne 03 8344 5062 Email [email protected] Project Summary 6. Building Skills & Capacity This project will utilise a crop rotation trial currently implemented by the Facey Group in the WA wheat-belt. Crop disease status will be monitored to evaluate the rise and fall of the population under varying crop rotations through time. Multiple diseases present in the trial will be monitored using the SARDI DNA probe (Predicta B). Soil borne pathogens causing significant crop disease, such as Take-all, Root rot, Crown rot and Rhizoctonia will be evaluated. Study results will be used to inform the disease module of the crop sequencing economic model (LUSO). 192 This land use sequencing optimiser model was developed under a previous GRDC project. Further development continues, to evaluate crop sequencing decisions throughout Australia. This project will provide vital, locally specific information to LUSO and a better understanding of the likely economic and biotic impact that crop disease inflicts on the individual crops and the crop rotation as a whole. Embedding a more accurate prediction of the disease status into the optimisable decision tool will enable improved integration of the disease susceptibility and expected impact on crop growth and yield. This project will help demonstrate the benefits of this model and opportunities available to grain producers using their own data. GRDC National Region Contract 26/02/2012 Start Contract End Contact Dr. Roger Lawes Organisation Phone 25/02/2013 Project Code UHS10228 University of Western Australia 08 63336461 Email [email protected] Project Summary Water-use efficiency (WUE) in the high rainfall zone of Tasmania can be extremely variable, as a result of environmental and agronomic constraints to grain production that limit yield potential. Potential WUE, which takes into account losses due to runoff and drainage, has a maximum value of around 20 kg/ha.mm and is variable as a result of constraints of climate, soil attributes and water availability. irrigated farming systems in Tasmania’, and assess the effect of irrigation and applied N, as standard basal vs. slow release, to optimise crop growth and WUE of spring-sown barley. Slow release fertilisers are used overseas and mainly in Australian horticultural industries, but may be an alternative strategy in a high-rainfall environment such as Tasmania, to address timing issues. Yield, WUE and economic returns could be improved by applying additional N fertiliser with strategic irrigation, thus avoiding colimitation of these inputs. The proposed project will be linked to ‘Improved WUE in rainfed and N dynamics will be monitored during the project and there will be opportunity to follow up the results of the field trial in a glasshouse pot experiment during the 2012 teaching year. 193 6. Building Skills & Capacity UHS10228: Undergraduate Honours Scholarship - Deon Heys (UT) Effect of irrigation and N supply on water-use efficiency of barley UHS10229: Undergraduate Honours Scholarship - Elisha Thynne (ANU) Analysing the production risks in the white grain pathogen Botryosphaeria zeae GRDC National Region Contract 27/02/2012 Start Contract End Contact Dr. Tina Acuna Organisation Phone 26/02/2013 Project Code UHS10229 University of Tasmania 03 6226 7507 Email [email protected] 6. Building Skills & Capacity Project Summary 194 My proposed Honours project will be to analyse wheat grain infected with the fungal pathogen Botryosphaeria zeae in an attempt to determine whether Botryosphaeria zeae produces any mycotoxins. Botryosphaeria zeae has contaminated a significant amount of wheat seed in Queensland, and whilst this causes grain discolouration, it is currently unclear as to whether Botryosphaeria zeae produces any mycotoxins. Mycotoxins are metabolites produced by many different types of fungi. The consumption of mycotoxins via fungal-infected foods, such as contaminated wheat grain, has been associated with a number of severe health problems. Because of the uncertainty as to whether Botryosphaeria zeae produces any mycotoxins or not, wheat infected with this fungi has been declared unsafe for human consumption. My project will involve culturing Botryosphaeria zeae and then using these cultures to infect wheat grain samples. The Botryosphaeriainfected grain samples will be ground-down and the metabolites present in the samples extracted using an appropriate organic solvent. The extracted sample will be analysed using modern detection methods such as liquid chromatography/time of flight mass spectometry (LC/TOF-MS) in order to assess the presence of any known mycotoxins. GRDC Region National Contract 30/01/2012 Start Contract End Contact A/Prof. Peter Solomon Organisation Phone 29/01/2013 Project Code UHS10232 Australian National University 02 6125 3952 Email [email protected] Project Summary The project will test how modern soil wetting agents affect the pasture growth in an area representative of forest gravel pasture and cropping soils in the South West of Western Australia. The project will investigate the economic benefit of applying the products to pasture and cropping systems, taking into account how the products affect total dry matter production and individual species dry matter production in pastures, and through laboratory work apply these findings to yield potential of cropping systems in the same soils. The field trial will be conducted on gravel loam soil in the Shire of West Arthur, an area transitioning from total livestock production to extensive grain production. Product will be applied to the chronically water repellent soil at rates of 0%, 50%, 100%, 150% and 200% of recommended rate and be measured over a period of two seasons. In addition, laboratory work will investigate the effect of variation of rate on the dry matter production and yield potential of cereals. An extensive economic analysis will be conducted at the conclusion of the project to determine the economic effect of varying the rate of product. 195 6. Building Skills & Capacity UHS10232: Undergraduate Honours Scholarship - James Campbell (UWA) Effect and economic benefit of rate of soil wetting agent in gravelly loam pasture and cropping systems. UHS10234: Undergraduate Honours Scholarship - Jasmine Hart (UF) Interactions of actinobacteria with rhizobia GRDC National Region Contract Start 27/02/2012 Contract End Contact Dr. Ken Flower Organisation Phone 26/02/2013 Project Code UHS10234 University of Western Australia 08 6488 4576 Email [email protected] Project Summary 6. Building Skills & Capacity Endophytic actinobacteria have been isolated from crop plants and the laboratory I will join has developed some of these to control fungal root pathogens of cereal plants. We are seeking to employ a similar strategy for legumes, where there is a strong need to control fungal diseases as well as to improve germination and establishment. Actinobacterial endophytes have been isolated from leguminous plants such as peas, lucerne and lupins and strains that can control pathogens of legumes have been identified by a fellow student. 196 In my project I will study the interaction of the effective actinobacteria with the rhizobia associated with the host plant. The specific functions to be examined will be the effect on nodule development, the efficiency of nitrogen-fixation, and changes in nodule and root architecture. Other factors will be the method of addition of the actinobacteria to the seed or roots, and the time of addition vis-a-vis the introduction of the rhizobium. GRDC National Region Contract 27/02/2012 Start Contract End Contact Prof. Christopher Franco Organisation Phone 26/02/2013 Project Code UHS10235 Flinders University 08 72218554 Email [email protected] Project Summary This project will examine the influence compost has on soil microbial communities. Soil processes such as phosphorus and iron solubilising, sulphur mineralisation nitrogen fixation as well as aiding in the uptake of various other macro and micro nutrients. Soil biology can influence plant physiology through the excretion of hormones and reduced incidence of disease. The compost industry has gained momentum and is marketed on the back of such claims. This project will evaluate changes in the microbial community following the addition of compost. Subsequent community analysis will be performed through DNA extracted from the soil samples, prior to planting, 3 leaf stage, 5 leaf stage, booting , anthesis, grain fill, and post harvest. This will, inturn determine the resiliance of the compost induced changes on bacteria, fungi, and archaea. A liquid compost extract as well as solid compost and a control will make up the experimental design. The proposed crop, sorghum, is the dominant summer crop grown throughout northern NSW and southern Queensland. The trial will be conducted on a both the red (dermosol) and black (vertosol) soils of the Liverpool plains in order to determine whether soil type imparts an additional interaction effect. As well as microbial analysis, other crop parameters will be examined including biomass and grain yield, leaf nitrogen percentage, indicating an association between soil biota and nutrient uptake efficiency. The soils ability to govern and constrain crop production is highly influenced by soil biota therefore it is imperative that further research be conducted in this emerging field. 197 6. Building Skills & Capacity UHS10235: Undergraduate Honours Scholarship - Jeremy Brown (US) In crop assessment of soil microbial communities following compost application UHS10236: Undergraduate Honours Scholarship - Joanna Lang (UWA) A financial analysis of on-farm grain storage and handling in Western Australia GRDC National Region Contract 01/01/2012 Start Contract End Contact Assoc Prof. Michael Kertesz Organisation Phone 31/12/2012 Project Code UHS10236 University of Sydney 02 8627 1022 Email [email protected] 6. Building Skills & Capacity Project Summary 198 Increased marketing opportunities bought on by deregulation of grain markets in 2004 have led to a large interest by growers in on-farm storage systems. On-farm storage improves farmers? ability to exploit increased marketing opportunities as well as better control their grain quality. Applying grain management procedures during storage allows farmers to capture premium prices by being able to supply quality assured grains direct to end users. Sourcing high quality, traceable grain is pivotal in a future driven by supply chain integrity. A model will be used in this project to show the costs and benefits available to farmers if invested in on-farm storage infrastructure. The project will examine the current successful on-farm storage facilities on the East coast and investigate whether the development is a viable option for Western Australian growers. The range of costs and benefits considered in the analysis will include: initial investment costs, increased quality rewards, lower warehousing and transport costs, increased flexibility at time of harvest, increased marketing opportunities, increased pest control and opportunity costs. A comprehensive sensitivity analysis will also be undertaken to test how different factors directly and indirectly impact its effectiveness. GRDC Region National Contract 27/02/2012 Start Contract End Contact . Mugera Organisation 26/02/2013 Project Code UHS10237 University of Western Australia Phone Email Project Summary Most current commercial cultivars of bread wheat are susceptible to Crown Rot, a potentially virulent pathogen that can significantly reduce yield through shrivelled grain and senescense of tillers. The ability of cultivars to maintain yield protential in the presence of crown rot is determined both by resistance/tolerance to the pathogen per se, and to the level of post-anthesis mositure stress experienced by the crop. Therefore, we will seek to investigate relationship between water-use efficiency in a double haploid population (2-49 x W21MMT70, n=208 - Refer Bovill et al. 2010, TAG 121:127136) that has been previously phenotyped for crown rot resistance. The population will be assessed using a novel glasshouse assay that directly measures water use and biomass accumulation, and in the field at Gatton. Data will be collected on yield, grain size, canopy temperature, stomatal conductance, chlorophyll content and StayGreen. Results will be gathered from a field trial in Gatton and three temporal replications in a glasshouse at St Lucia. A relationship between QTL for water use efficiencey and Crown Rot resistance of the genotypes will be analysed. This project is linked to GRDC project UQ00049 and the Crown Rot Initiative. 199 6. Building Skills & Capacity UHS10237: Undergraduate Honours Scholarship - Karen Shears (UQ) A quantitative approach relating crown-rot resistance to water-use efficiency and grain yield in wheat (Triticum aestivum) UHS10238: Undergraduate Honours Scholarship - Kimberley Duver (UNE) Application of mixed waste organic outputs to cropping soils - potentialbenefits and pitfalls: impacts on soil biology and function" GRDC Region National Contract 01/02/2012 Start Contract End Contact Dr. Mark Dieters Organisation Phone 31/01/2013 Project Code UHS10238 University Of Queensland 07 3365 7410 Email [email protected] Project Summary 6. Building Skills & Capacity In NSW, mixed waste organic outputs (MWOO) are currently being generated from the waste stream for application as a compostlike soil amendment. MWOO products are being promoted as a soil conditioner, as a means of adding carbon to soils and enhancing soil biological activity. 200 Due to the large quantities of these waste products being produced, broadacre application to grains and cropping soils is being encouraged. These claims however, have not yet been adequately tested and require rigorous assessment to ensure maximum benefit to the grains industry whilst minimising potential harm. Using an established pot experiment at UNE, and a range of analysis techniques (e.g. microbial biomass, respiration) this project will assess MWOO effects on soil biology, health, function, focusing on the nature and change, improvement or decline in microbial populations in MWOO amended soils. The project will examine a range of key NSW grain-growing soils (predominantly from the northern grains region) and will consider the effect of application rate and application methods on soils. The project forms part of a larger research program (supported by NSW State Government) to evaluate potential contamination of soils and soil water and the uptake of contaminants by plants where MWOO is applied to land. UHS10241: Undergraduate Honours Scholarship - Marcela Cespedes (USA) Image fusion for phenotypic analysis of plant images Contract Start 20/02/2012 Contract End Contact . Susan Wilson Organisation Phone 19/02/2013 Project Code UHS10241 University Of New England (02) 6773 2789 Email [email protected] Project Summary The aim of my honours project in the field of Phenomics titled 'Image fusion for phenotypic analysis of plant images' is to use mathematical methods and image analysis to develop ways to deduct vital plant statistics and information such as; classification of plant species, plant mass both wet and dry, inclusive and exclusive of root system, calculate its growth rate throughout the life cycle of the plant, moisture content and many other characteristics from photo images taken. This new method of gathering plant information will provide quick, efficient and reliable information for biologists and scientists. This will help develop new strains of grain crops which will be able to better tolerate some of Australia's current environmental conditions such as drought and high salinity content to name a few. Currently a common method to gather plant statistics and information is through destructive experiments which is time consuming as each plant in an experiment is individually handled, this limits the capacity to conduct trials on new grain species. Additionally in order to gain as much information as possible, this method requires the destruction of the plants and so eliminates any possibility to further study the growth stages of the plants in the trial. Through analysis of photo imaging, this nonintrusive method will preserve plants in the trial for future use while also enable us to gather the required information. 6. Building Skills & Capacity GRDC National Region 201 UHS10242: Undergraduate Honours Scholarship - Melissa Ann Coventry (UA) Can rye genes be used to ‘supercharge’ aluminium tolerance in wheat and barley? - A transgenic approach to dissect biochemical and biological properties of organic anion transporters from rye a GRDC National Region Contract Start 27/02/2012 Contract End Contact . Jinhai Cai Organisation Phone 26/02/2013 Project Code UHS10242 University of South Australia 08 830 25533 Email [email protected] Project Summary 6. Building Skills & Capacity Aluminium-toxicity tolerant varieties express ALMT1 transporters to allow secretion of organic acids from the root tip to detoxify surrounding Al3+ cations. 202 While an ALMT1 locus in rye controls secretion of malate and citrate, the corresponding ALMT1 locus in wheat only controls the secretion of malate. Does this reflect wider substrate specificity of these rye ALMT1s, or a higher concentration of citrate in the rye root tip compared to wheat? These questions will be addressed by 1. comparing the spectrum of organic acids secreted from hydroponicallygrown barley and wheat plants transformed with wheat or rye ALMT1s, and 2. Comparing the concentrations of individual organic acids within the root tips of wheat and rye. Transgenic lines are available and confirmed to be tolerant. Among the cultivated Triticeae cereals rye is one of the most aluminium tolerant. If this corresponds to rye ALMT1s being able to transport a wider range of organic acids than those from wheat, then it should be investigated if rye ALMT1s can be used to bolster aluminium tolerance in wheat and barley. This will be investigated by comparing tolerance levels of transgenic plants containing either the wheat or rye ALMT1s, using hydroponic root-length assays and pot-grown plants. GRDC National Region Contract 20/02/2012 Start Contract End Contact Dr. MONICA Ogierman Organisation Phone 19/02/2013 Project Code UHS10244 University of Adelaide 883036725 Email [email protected] Project Summary Stagonospora nodorum is the causal agent of glume blotch disease on wheat. A recent survey of wheat diseases in Australia rated glume blotch as the 3rd most important in terms of economic losses. Groundbreaking studies in this country by the labs of Dr. Peter Solomon and Prof. Richard Oliver have made in key breakthroughs in understanding the molecular basis of this disease. What though has yet to be established is the nutritional basis of the pathogen during infection i.e. what are the metabolic requirements of the pathogen during infection? Studies of the disease in Dr. Solomon?s lab have shown that the abundance of the compound gamma-aminobutyric acid (GABA) increase during infection of S. nodorum on wheat. In my honours project, I intend to determine whether or not S. nodorum GABA metabolism is important for infection. This will be done by inactivating the genes required for GABA synthesis (glutamate decarboxylase) and GABA breakdown (succinate semialdehydedehydrogenase). The resulting pathogen strains lacking these genes will then be infected on susceptible wheat varieties to determine whether or not GABA metabolism is required for infection. This is a proven approach in Dr. Solomon?s lab that has uncovered multiple pathogen genes required for infection. 203 6. Building Skills & Capacity UHS10244: Undergraduate Honours Scholarship - Oliver Mead (ANU) An analysis of gamma-aminobutyric acid metabolism in the wheat pathogen Stagonospora nodorum UHS10338: Undergraduate Honours Scholarship - Nadine Nolan (QUT) Immigration levels of C.ferrugineus GRDC National Region Contract Start 31/01/2012 Contract End Contact A/Prof. Peter Ssolomon Organisation Phone 30/01/2013 Project Code UHS10338 Australian National University 02 6125 3952 Email [email protected] Project Summary The flat grain beetle Cryptolestes ferrugineus is a major pest of stored grains. The capacity to detect C. ferrugineus in grain bulks has becoming increasingly important since this species has been shown to exhibit high levels of resistance to Phosphine. 6. Building Skills & Capacity The abundance and spatial distribution of insects through a grain bulk will influence the sampling intensity needed to detect the those insects (Elmouttie et al 2010). Clearly, characterisation of the abundance and levels of clustering of insect species in stored grain is critical to the development of accurate sampling strategies. 204 The abundance and spatial distribution of C. ferrugineus in an infested grain bulk will depend on two major factors: the rate of immigration of the insect into the grain, and the subsequent reproduction and clustering behaviour of the insects once immigration has occurred. In this project, small quantities of grain will be established for a brief period in grain fields and near grain storages in order to estimate the presence of, and any differences in, immigration levels of C. ferrugineus into the grain. After infestation, these small bulks will be examined to characterise the fine resolution, three dimensional spatial distribution of insects in the bulk. UHS10356: Undergraduate Honours Scholarship - Michelle Low (UCS) PBA Health Benefits of Australian Pulses Contract Start 27/02/2012 Contract End Contact Dr. Grant Hamilton Organisation Phone 26/02/2013 Project Code UHS10356 Queensland University of Technology +61 7 3138 2318 Email [email protected] Project Summary There have been a number of reports confirming that some pulses may be good sources of antioxidants. These antioxidants have been demonstrated to prevent a range of diseases including cancer, diabetes, atherosclerosis and other cardiovascular diseases. However a comprehensive study of the level of antioxidants in pulses typically grown in Australia and the impact of climate and growing conditions on antioxidant levels has not been undertaken. This project will investigate antioxidant levels typically found in Australian grown chickpeas, faba beans and field peas. The site variation in sampes will be investigated to determine the impact of enviroment on antioxidant levels. In the case of faba beans, a set of samples grown under a range of UV levels will be studied to determine the effect of UV on antioxidant levels. 6. Building Skills & Capacity GRDC National Region 205 UHS10360: Undergraduate Honours Scholarship - Kyle Reynolds (UCS) PBA Health Benefits of Australian Pulses GRDC National Region Contract Start 27/02/2012 Contract End Contact A/Prof. Chris Blanchard Organisation Phone 26/02/2013 Project Code UHS10360 Charles Sturt University 02 69332364 Email [email protected] Project Summary Project Plan: 6. Building Skills & Capacity The student will work on a project aimed at better understanding the functional properties of compounds contained in the various pulses. This would build on the successful project, which recently demonstrated the anti-cancer, anti-diabetes and anti-obesity properties of faba bean constituents. The project would also 206 look at the potential for pulse extracts to be used in the pharmaceutical industry, particularly in their use in the emerging field of regenerative medicine as regulatory compounds to promote the differentiation of adult stem cells to functional tissue such as bone and cartilage. GRDC National Region Contract 27/02/2012 Start Contract End Contact A/Prof. Chris Blanchard Organisation Phone 26/02/2013 Project Code UHS10403 Charles Sturt University 02 69332364 Email [email protected] Project Summary This project will determine if there is an early growth and potential yield benefit in using superphosphate as a fertiliser in acidic soil where aluminium toxicity is problem, compared to ammonium based fertilisers. Wheat growth in response to two types of fertilisers will be compared. 207 6. Building Skills & Capacity UHS10403: Undergraduate Honours Scholarship - Brenton Leske (UWA) Reducing the impact of aluminium toxicity using superposphate as a seeding fertiliser UHS10410: Undergraduate Honours Scholarship - Kimberley Adams (UWA) Improving crop establishment in non-wetting soils through stubble management GRDC National Region Contract 29/01/2013 Start Contract End Contact Dr. Jason Able 28/01/2014 Project Code UHS10410 Organisation Phone 08 8303 7075 Email [email protected] Project Summary 6. Building Skills & Capacity Along the south coast of Western Australia non-wetting soils are a major limiting factor in crop production. Due to the sandy soils of this region the majority of the farmers are also zero or minimum till with full or partial stubble retention. Among the farming community in this area there are conflicting thoughts about the affect stubble retention has on the nonwetting nature of the soils. With this research 208 I will be looking at how different stubble management practices affect non-wetting soils in the south coast region. In particular I will determine how burning stubble, full stubble retention, extra think stubble and trampled stubble affect the water repellence of soils and the subsequent effects on crop germination and establishment. I will be measuring rainfall, soil water repellence, soil moisture content, soil infiltration and soil carbon before and throughout the growing season. All the plots will be seeded with a no-till seeder to minimise soil and stubble disturbance and I will have 4 replicates of each treatment. After seeding I will do crop emergence counts and towards the end of the growing season crop biomass will be measured. GRDC National Region Contract 25/02/2013 Start Contract End Contact Dr. Ken Flower Organisation Phone 24/02/2014 Project Code UHS10411 University of Western Australia 08 6488 4576 Email [email protected] Project Summary This project will make use of new magnetic probes to monitor the effects of temperature and drought on plant water status in wheat. While rainfall is the predominant limitation in Australian cropping systems, elevated temperatures and more frequent droughts will provide additional challenges for grain growers as our climate changes. Much of the current work on water relations in wheat has used intrusive/destructive sampling methods or temporal measurements, often providing an incomplete picture of plant water relations. Using leaf patch clamp pressure (LPCP) probes it's possible to continuously monitor leaf water status in real time to reveal shortterm and long-term dynamics in plant water status. This has already been trialled on wheat, but its potential has not yet been evaluated. By using the probes (in conjunction with other measurements), I intend to determine the point at which wheat plants make physiological adjustments to conserve water during different stages of drought and in response to high temperatures. This project will monitor water status in wheat genotypes with contrasting water use efficiency (WUE) and new breeding lines from Intergrain to determine the relationship between leaf temperature, transpiration and plant water status during drought and high temperatures. 209 6. Building Skills & Capacity UHS10411: Undergraduate Honours Scholarship - Liam Ryan (UWA) Monitoring the water status of wheat using novel magnetic probes UHS10416: Undergraduate Honours Scholarship - Wesley Lefroy (UWA) Does knowledge of the soil map improve the utility of remotely sensed soil information? GRDC National Region Contract 25/02/2013 Start Contract End Contact Dr. Helen Bramley Organisation Phone 24/02/2014 Project Code UHS10416 University of Western Sydney 08 6488 1539 Email [email protected] Project Summary 6. Building Skills & Capacity Remotely sensed information is a relatively new and exciting frontier for precision agriculture. The use of gamma ray spectrometry and electromagnetic induction provides rapid, non-invasive and high resolution soil mapping. Current issues with the techniques surround the accuracy of mapping subsoil. In the majority of soil types, remote sensing methods cannot accurately map subsoil, failing to identify constraints that may inhibit root growth. 210 The aim of this study to find whether the added knowledge of a direct soil survey can improve the utility of remotely sensed soil information. An analysis of the relationship between direct soil survey data and radiometric data will form the basis of this research. The soils being examined are high and low yielding, illustrated by over 10 years of yield mapping data as provided by the farmer. The research location is a 300ha piece of land in Tammin, covering up to six different soil units, representative of a wide area of soil types across the Western Australian wheatbelt. It is hypothesized that the relationship between survey data and radiometric data will vary, dependent on the nature of the sub-soil. It is expected that the most influential sub-soil characteristics are pH, electrical conductivity, texture, gravel percentage, and the presence of a hardpan, which may limit root depth in some soil types. GRDC Region National Contract 01/01/2013 Start Contract End Contact Professor. Robert Gilkes Organisation Phone 31/05/2013 Project Code UHS10418 University of Western Australia 08 6488 2509 Email [email protected] Project Summary Sorghum is an important summer grain crop due to its ability to tolerate heat and water stress. However, effective use of sorghum as a feed grain would be improved by larger/ less variable grain size. Furthermore, grain-fill is adversely affected by abiotic stresses that are increasingly due to climate change. Understanding the biochemical mechanisms that regulate grain-fill and grain size is crucial for developing molecular markers assisting breeding programs to optimise grain size and yield stability. Work in Dr Nonhebel's laboratory at UNE has been investigating sugar and plant hormone signalling during grain-fill in rice. A cluster of co-expressed genes encoding auxin biosynthesis genes as well as other signalling proteins have been identified as important for optimal post-pollination grain-fill. I will be investigating the sorghum orthologues of these genes. Gene expression as well as biological activity of the protein products will be investigated during grain-fill and compared to grain size. The effect of heat stress on gene expression will also be monitored. Bioinformatic tools will be used to screen existing sequence data to identify polymorphic genes. This work will provide the foundation for future studies in which the genetic polymorphisms in grain-fill genes can be developed as molecular markers. 211 6. Building Skills & Capacity UHS10418: Undergraduate Honours Scholarship - Benjamin Schreiber (UNE) Signalling and Nutrient Partitioning during Sorghum Grain-fill Under Stress: Informing Breeding Strategies UHS10444: Undergraduate Honours Scholarship - Adam Norman (UA) A genetic dissection of physical grain quality in wheat GRDC National Region Contract Start 01/07/2013 Contract End Contact Dr. Heather Nonhebel Organisation Phone 30/04/2014 Project Code UHS10444 University Of New England 02 6773 2083 Email [email protected] Project Summary This project will focus on how grain size (screening levels) and hectolitre weight are affected by genotype and environmental interactions and will be carried out with AGT. The variety Janz is notorious for having small grain size, with this trait being carried through to its descendants. In this project, two Janzbased populations (Kukri/Janz DHs and Janz/Frame F2:5) will be used to identify the key quantitative trait loci controlling small grain size in Janz. 6. Building Skills & Capacity The Cre1 resistance allele present in varieties such as Silverstar is considered to be very effective in controlling cereal cyst nematode numbers. However, the presence of this allele 212 is also associated with a reduction in grain size. It is unknown whether this effect is caused by the Cre1 locus itself (pleiotropy) or by another nearby locus (linkage). This project will aim to characterise the effect of the Cre1 allele on grain size and determine whether it is due to pleiotropy or linkage. When Grain Trade Australia tightens its APW specification for hectolitre weight (HLW) from 74 to 76 kg/hl, varieties with HLW issues such as Correll may fail to meet standards on a regular basis. Correll’s HLW issues may be due to genetic control, or to grain shape, and this project will investigate this. GRDC Region National Contract 29/01/2013 Start Contract End Contact Dr. Haydn Kuchel Organisation Phone 28/01/2014 Project Code UHS10446 University of Adelaide 08 8313 7708 Email [email protected] Project Summary Ascochyta blight of lentils is a major yield constraint. The characterisation of the specific mechanisms involved in host specificity and pathogenicity will aid in the development of new resistant crop cultivars and methods of control. Like other related necrotrophs, the fungus is believed to produce host-specific necrotic effectors which interact with host receptors to cause infection. In previous research conducted by myself as a component of my undergraduate degree I was able to narrow down the laboratory procedures needed to grow A. lentis to induce the secretion of proteins and metabolites that induce necrosis on the susceptible lentil cv Digger. The proposed research project will involve further characterisation and isolation of the effector candidates, and determination of host specificity of the compounds secreted in culture filtrates. Characterisation of the secreted products will involve methods of size fractionation, heat and pronase treatments, and subsequent protein sequencing to determine the candidate’s nature and identity. The level of host specifcity of effectors will be tested both at the species (susceptibility of other crops such as chickpea, field pea and faba bean) and at the intra-specific level. 213 6. Building Skills & Capacity UHS10446: Undergraduate Honours Scholarship - King Yin Lui (CUR) Dissection of genetic factors affecting Ascochyta lentis pathogenicity and its interaction with lentil (Lens culinaris) UHS10454: Undergraduate Honours Scholarship - Benjamin Romeo (US) The effect of slow release nitrogen fertilisers on biomass production, crop nitrogen content, grain yield and grain protein concentration GRDC Region National Contract 04/03/2013 Start Contract End Contact Dr. Judith Lichtenzveig Organisation Phone 03/03/2014 Project Code UHS10454 Curtin University of Technology 08 9360 1311 Email [email protected] Project Summary 6. Building Skills & Capacity The project will be conducted in the Southern Highland region of NSW, using the dualpurpose milling-quality wheat EGA Wedgetail. The aim is to evaluate the effect of different application rates of slow release nitrogen fertilisers on crop biomass production, plant nitrogen content, grain yield and grain protein levels. 214 Slow release nitrogen fertilisers, which are extensively used in the turf industry, are based on a urea nutrient core with a sulphur polymer membrane, releasing nitrogen for up to 6 months following application. My field experiment will consist of four treatments (two products with the Poly - S technology [Everris, formerly known as Scotts], one product with Entec Technology [an ammonia stabiliser by Incitec Pivot Fertilisers] and one product without any coating, e.g., urea) and an unfertilised control. A randomised block design will be used, replicated three times. All treatments will be sown with phosphorus (as superphosphate) and potassium (as potassium sulphate) as starter fertiliser with five different rates of nitrogen applied after germination (0 kg/ha [control], 50 kg/ha, 100 kg/ha, 150 kg/ha and 200 kg/ha). The wheat will be grazed three times before being allowed to produce grain. Measurements will include dry matter amount after each grazing, leaf nitrogen content (SPAD chlorophyll and Greenseeker meters), grain yield, aboveground dry biomass at maturity and grain protein percentage. GRDC Region National Contract 01/01/2013 Start Contract End Contact Dr. Daniel Turner Organisation Phone 15/12/2014 Project Code UHS10456 University of Sydney 02 8627 1052 Email [email protected] Project Summary Invasive snails and slugs are a constant and intractable pest to Australian native fauna, upsetting fragile ecosystems as well as destroying beneficial crops. Combined, they are estimated to be responsible for ~25-50% of annual crop loss, leading to widespread detrimental economic impacts to the grains industry (~$5 billion annual crop or 17.4% of total Australian exports). While a variety of measures are currently undertaken to control these pests (baiting, bashing and burning), these methods have proven to be overall ineffective. Our limited understanding of key factors involved in regulating reproductive success, where a single snail can produce up to 400 offspring, means that we are helpless to curb their current and future population growth. This project will take advantage of the USC's snail gene database to provide the first comprehensive molecular investigation into the functioning of both genes and hormones used by these invasive pests that trigger maturation, courtship and mating. The discovery of and testing of these hormones from this study will provide important information for the industry toward the isolation of and chemical sythesis of hormone based contraceptive control agents that are specific for pest snails and slugs. 215 6. Building Skills & Capacity UHS10456: Undergraduate Honours Scholarship - Bradley Harding (Uni Sunshine Coast) Development of molluscan contraceptives: Investigation into the molecular basis of reproduction in invasive crop snails and slugs UHS10457: Undergraduate Honours Scholarship - Clare Flakelar (CSU) Components in Canola Oil GRDC National Region Contract Start 18/02/2013 Contract End Contact Dr. Scott Cummins Organisation Phone 17/02/2014 Project Code UHS10457 University of Sunshine Coast 07 5456 5501 Email [email protected] Project Summary The project will be complementary to work conducted this year involving quantitative determination of tocopherols and carotenoids. The results from this research and a lack of literature have indicated research into levels of lutein in canola oil has not been previously entered in great depth. We have found lutein is present in varying amounts of canola varieties. Lutein is a compound responsible for aiding in the prevention of macular degeneration. 6. Building Skills & Capacity This project will look into levels of components of canola oil, namely lutein and how conditions altered can affect these levels. The analyses 216 conducted will be aimed toward maximising lutein levels (while not diminishing tocopherols or other carotenoids) in canola in order to obtain canola oil will enhanced health benefits. Conditions including but not limited to; oil yield, climate, Free Fatty Acid (FFA), soil type, fertiliser type/additional chemicals and Genetically-Modified (GM) will be investigated. The process of oil extraction may also be investigated to ensure no detrimental effects from this process. UHS10458: Undergraduate Honours Scholarship - David Zadow (UWA) Grain Profitability maps for Western Australia Contract Start 04/02/2013 Contract End Contact Dr. Paul Prenzler Organisation Phone 03/02/2014 Project Code UHS10458 Charles Sturt University +61 2 6933 2978 Email [email protected] Project Summary Wheat is the main crop grown by WA farmers. Currently farmers have access to models which give seasonal yield estimates for different shires. However these models only tell half of the picture when it comes to farm planning and risk management. The other half relates to the price risks associated with producing wheat, along with the costs of production. The expected yield can change within a season, but so can the price of wheat and their combination, net of the costs of production, gives the profit from wheat production. My project aims to combine seasonal yield predictions with seasonal price forecasts and localised costs to create shire profitability maps for Western Australia. Further, by conducting historical analyses we will be able to test how sensitive are wheat profits in different shires to changes in wheat yields, wheat prices and costs of production. We intend to create maps which will show the relative profit potential of each shire both as the season progresses and when compared to previous years. The project will provide information to farmers and investors who want to compare the profitability of wheat production (and its riskiness) in different shires in WA. Such people will be able to use the model to determine whether the price of land in a particular shire reflects its profit potential. 6. Building Skills & Capacity GRDC National Region 217 UHS10469: Undergraduate Honours Scholarship - James Hughes (UW) Improved methods for the analysis of disease ratings, with application to the GRDC funded Crown Rot project GRDC Region National Contract 25/02/2013 Start Contract End Contact Prof. Ross Kingwell Organisation Phone 24/02/2014 Project Code UHS10469 University of Western Australia 08 9368 3225 Email [email protected] Project Summary 6. Building Skills & Capacity Crown rot is a major disease of winter cereal crops (bread wheats, barley, durum) in Australia, particularly in north-eastern and parts of southern Australia. Disease can be particularly severe in field situations where there is a high load of infected crop residue from the previous season, moist conditions early in the season which favour infection and then a dry finish that stresses already infected plants. 218 Current methodologies for screening crown rot susceptibility/resistance have been developed at various sites across Australia with these methods embedding the framework of generalised linear models (GLMs). However, Collins (2008) compared the performance of estimation methods for generalised linear mixed models (GLMMs) and concluded that the approximate methods such as PQL performed well when compared to Bayesian methods for the analysis of ordinal data. The aim of the project will be to examine these findings in more detail using real and simulated data-sets. We will be particularly focused on the bias and accuracy of estimates of variance parameters as well as examining the behaviour of tools of inference involving fixed effects. UHS10479: Undergraduate Honours Scholarship - Deborah Bud (UW) Improved Canola Variety Information for Farmers Contract Start 04/03/2013 Contract End Contact Prof. Brian Cullis Organisation Phone 03/03/2014 Project Code UHS10479 University of Wollongong 02 4221 5475 Email [email protected] Project Summary The aim of this project is to think of new and innovative designs in comparing the varieties of Canola. The information gathered from this project will be used as part of the National Variety Trials that will aide farmers in choosing between Canola varients. that are currently in use do not offer a valid comparison between varieties of different chemistry groups so this project will address that issue in developing designs that can validly compare Canola varieties from the same or different chemistry groups. For this project the varieties of Canola that will be compared include convential, roundupready, triazine-tolerant and imidazolinone tolerant varieties. The Chemistry block designs These designs will be easy to sow, and allow for efficient and error-free trial management. 6. Building Skills & Capacity GRDC National Region 219 UHS10480: Undergraduate Honours Scholarship - Stefan Schmitt (UA) Genetic Control of Heat Tolerance in Wheat GRDC National Region Contract Start 29/07/2013 Contract End Contact Prof. Brian Cullis Organisation Phone 28/07/2014 Project Code UHS10480 University of Wollongong 02 4221 5475 Email [email protected] Project Summary This project will characterise reproductive heat stress tolerance in exotic wheat germplasm which has previously been shown to convey adaptation to high temperature stress, greater than many existing wheat varieties. 6. Building Skills & Capacity Mapping populations which have been developed from crosses between adapted Australian varieties, and land races with putative heat stress tolerance, will be used as the basis of this study. Lines will be grown under controlled environment conditions, subjected to heat stress during reproductive growth, and then evaluated for their physiological responses. 220 In addition, these lines will be grown in the field and their basic agronomic performance (plant height, grain size, maturity etc) evaluated. A genetic map will be constructed, and the phenotypic data used to identify QTL (genes) which control heat stress tolerance. It is hoped that the results from this project will lead to the development of heat tolerant germplasm able to be introgresed into current breeding lines, and the breeding tools required to achieve high rates of genetic gain. UHS10485: Undergraduate Honours Scholarship - Daniel Ahfock (UQ) Quantifying uncertainty in genetic map construction Contract Start 29/07/2013 Contract End Contact Dr. Haydn Kuchel Organisation Phone 28/07/2014 Project Code UHS10485 University of Adelaide 08 8313 7708 Email [email protected] Project Summary Genetic studies are a key element of modern crop breeding, facilitating more rapid and economical plant improvement than traditional schemes. Through these studies, associations detected between genetic markers and traits such as quality and yield can be incorporated into the breeding process. Constructing a genetic map is the starting point in finding marker-trait associations, and as such, errors in this stage can complicate further analysis and lead to spurious results. Many map construction approaches have been developed which focus on estimating the order and positioning of a set of genetic markers. However, little work has been done on quantifying map uncertainty, and map verification is often an ill-defined labour- intensive task. Methods to objectively characterise the uncertainty in a genetic map will improve existing map construction techniques and streamline further analysis. This project will develop statistical techniques to evaluate the quality of a genetic map and investigate methods for refining maps while accounting for uncertainty in estimation. In particular, I will consider high-density map construction, where the large number of closely spaced markers increases both the computational complexity and uncertainty in estimation. The methods developed will be tested on simulated data and data from a large wheat mapping population. 6. Building Skills & Capacity GRDC National Region 221 UHS10488: Undergraduate Honours Scholarship - Huw Ogilvie (ANU) Optimising root architecture for yield and environmental benefits: identifying genetic and epigenetic targets by manipulating peptide relayed signals GRDC Region National Contract 25/02/2013 Start Contract End Contact Dr. Emma Huang Organisation Phone 24/02/2014 Project Code UHS10488 University Of Queensland 07 3833 5542 Email [email protected] Project Summary 6. Building Skills & Capacity The Djordjevic Lab has discovered a novel family of peptides, regulators of root architecture (RAR), that appear to modify root architecture as part of stress response, including in relaying communication of stress conditions with phytohormones. Altering root architecture has been found to increase water use efficiency, nutrient uptake and plant biomass. 222 My project will begin with measuring changes in root architecture in response to RAR and hormone treatment across a combination of RAR- and hormone-defective lines. The molecular biology of these changes will be analysed using RNA-seq technology, where the expression levels of all genes are analysed at once. Genes which change expression levels in response to hormone or RAR treatment, but not in RAR- or hormonedefective lines, will be identified using bioinformatics tools. I intend to begin this work in Arabidopsis, but quickly adapt it to commercially important crops. Furthermore, adaption to stress can be maintained across generations of plants through epigenetic processes including DNA methylation. After collecting and analysing RNA-seq data, I will combine it with previous data on DNA methylation to identify RAR and root architecture related genes which may be regulated through DNA methylation. I will confirm this activity by using bisulfite conversion technology. UHS10504: Undergraduate Honours Scholarship - Mark Turner (UQ) RNA signalling and plant disease resistance Contract Start 29/01/2013 Contract End Contact Assoc Prof. Michael Djordjevic Organisation Phone 28/01/2014 Project Code UHS10504 Australian National University 02 6125 3088 Email [email protected] Project Summary Gene silencing is a highly conserved process in plants and animals. It is fundamentally important to gene regulation, disease resistance, adaptive response to the environment, and evolution. Gene silencing in plants and animals, can spread throughout the organism. Research in the applicant’s laboratory established micro-grafting in the model plant Arabidopsisas an approach to investigate the mechanisms, and to discover genes involved in intercellular spreading of gene silencing. This led to the discovery of small regulatory RNAs as intercellular, grafttransmissible gene silencing signals. More recent research in the applicant’s laboratory has identified that a gene called Pleiotropic Regulatory Locus 1 (PRL1)is required for intercellular spreading of gene silencing. PRL1 is highly conserved between plants and animals. Importantly, PRL1 had already been reported to be required for general plant defence, including resistance to bacterial and fungal pathogens in plants. These results suggest that intercellular RNA signalling plays a crucial in role general plant defence. The aim of this project is to identify additional genes involved in both intercellular RNA signalling and plant disease resistance. This will confirm our hypothesis that intercellular RNA signalling does indeed play a crucial mechanistic role in general plant defense. 6. Building Skills & Capacity GRDC National Region 223 UHS10509: Undergraduate Honours Scholarship - Sandra Whitmarsh (UA) Analysis and performance of key perennial forage shrub to improve production and sustainability in the mixed farming systems of the SA and Victorian Mallee GRDC Region National Contract 29/01/2013 Start Contract End Contact Assoc Prof. Bernie Carroll Organisation Phone 28/01/2014 Project Code UHS10509 University Of Queensland 07 3365 2013 Email [email protected] 6. Building Skills & Capacity Project Summary 224 Forage shrubs are increasingly recognised for their importance as complementary fodder reserves in marginal cropping environments. The ability of woody perennials to bring value to previously unproductive land, utilise out of season rainfall, and provide green feed during the autumn feed gap has been well established (Bulman 2004). Environmental services such as soil stabilisation, rehabilitation of chemically constrained areas, and reduction of recharge have also been welldocumented (de Koning & Milthorpe 2008). Around 85 percent of SA Mallee grain producers have a livestock component as part of their farm enterprise (Llewellyn et al 2010). Whilst grain production is the primary focus, add-ins such as forage shrubs can stabilise livestock production, with flow-on effects to the whole farm. The ability of shrubs to reduce supplementary feeding costs and provide protection and shelter to livestock has the potential to bring significant benefits to integrated crop/livestock farm enterprises (CRC Future Farm Industries Evercrop program). There are currently gaps in understanding the contribution of shrub plantations to farm profitability and the environmental services they are providing within the cropping areas of the Mallee. The proposed work will involve a regional survey of representative stands of forage shrubs within the Mallee regions of South Australia and Western Victoria. Assessments of forage growth and soils will be determined using soil surveys, dry matter estimations with a modified 'Adelaide' technique (reference) and photographic scoring tools and compiling site management history from farmer survey and interviews. The importance of forage grazing shrubs to these systems will be assessed using a suitable economic modelling program. UHS10527: Undergraduate Honours Scholarship - Alexander Pocock (UQ) Discovery of small RNA in the wheat transcriptome 225 Contract 01/03/2013 Start Contract End Contact Dr. Matthew Denton Organisation Phone 28/02/2014 Project Code UHS10527 University of Adelaide 08 8313 1098 Email [email protected] Project Summary I will investigate the diversity and roles microRNA play in wheat gene regulation. MicroRNA are important regulators of gene activity, acting through RNAi mechanisms, as has been shown in model plant organisms. However, the extent of microRNA diversity and their roles in adaptive processes in wheat is not known. I will address this by studying small RNA populations from bread and einkorn wheat seedlings under temperature and nutrient stress using next generation DNA sequencing and bioinformatics analyses. This will enable me to discover novel microRNA in wheat and investigate how microRNA expression patterns and abundance differ in shoots and roots. Furthermore, next generation sequencing will allow for in-depth quantitative comparison of microRNA abundance in wheat subjected to temperature or nutrient stress. This will uncover molecular pathways responsive to abiotic stress which rely on microRNA regulatory mechanisms. 6. Building Skills & Capacity GRDC National Region UHS10548: Undergraduate Honours Scholarship - Bonnie Hargreaves (UWA) Heritability of resistance to black spot disease in a field pea recurrent enrichment and association population (Pea-REAP) GRDC Region National Contract 22/07/2013 Start Contract End Contact Dr. Milos Tanurdzic Organisation Phone 23/07/2014 Project Code UHS10548 University Of Queensland 07 3365 2045 Email [email protected] Project Summary 6. Building Skills & Capacity The proposed project will investigate the heritability of resistance to black spot disease in field peas. The peas will be sourced from the very diverse experimental population PeaREAP (Pea Recurrent Enrichment and Association Population) which has been developed at UWA over the past 5 years. Diverse germplasm has been integrated into this population from Germany, Australia, China and wild/landrace peas with blackspot resistance; many of which originated in GRDC project UWA356. 226 This population has very broad diversity for blackspot disease resistance, stem strength and flowering time. Peas will be planted in a field trial at the UWA Shenton Park Research Station where they will be grown with pea straw that has been infected with black spot. The degree of susceptibility to black spot will then be measured in the peas and resistant individuals will be identified. Peas will also be tested for heritability of stem compressed thickness and flowering time. Molecular genetic markers based on candidate genes for disease resistance, stem fibre/architecture and flowering time control will be tested for genetic association with field scores. GRDC Region National Contract 25/03/2013 Start Contract End Contact Prof. Wallace Cowling Organisation Phone 24/03/2014 Project Code UHS10549 University of Western Australia 08 6488 7979 Email [email protected] Project Summary A growing need for increased production of food to match the growing human population is affected by abiotic stresses on crops. Increase in soil salinity is an abiotic stress that impacts plant growth and ultimately crop yield. Osmotic regulation is also an important factor that impacts on plant growth. Mechanisms of salinity tolerance have been studied greatly; however gaps still remain in some areas like the long distance signalling of stress response factors in plants. Phloem is a transport system and is involved in long distance signalling in plants. New advances in molecular technology allow powerful and sensitive analysis to be conducted on phloem sap in order to identify micro RNAs (a new candidate of signal molecule), metabolites and proteins. This study will use aphid stylectomy to obtain pure phloem sap from two different cultivars of wheat; a salt sensitive control and a salt tolerant cultivar. Analysis of phloem sap will be conducted coupled with specific physiological assays; such as plant growth, reducing sugar concentrations and osmotic potential of root and shoot. The ability to use the novel technique of micro RNAs detection in phloem provides a new way of looking how a wheat plant responds to a saline environment. 227 6. Building Skills & Capacity UHS10549: Undergraduate Honours Scholarship - Jai Strempel (UF) Micro RNAs and other phloem molecules coupled with physiological recordings of differing wheat cultivars under salinity stress. UHS10659: UHS - Candy Taylor (UWA) Understanding the role of floral integrator locus FT in controlling phenology in narrowleafed lupin for future breeding GRDC National Region Contract 17/02/2014 Start Contract End Contact Mrs. Sue Dodimead Organisation Phone 31/10/2014 Project Code UHS10659 University of Western Australia 08 6488 1973 Email [email protected] 6. Building Skills & Capacity Project Summary 228 Narrow-leafed lupin is an important grain legume for farming systems on acidic soils in the northern grainbelt of Western Australia. However, the region where this crop may be grown is restricted due to the very narrow phenological diversity of currently available commercial varieties, and is expected to continue contracting given the projected climate change for the South West. Therefore, it is important to increase diversity of current varieties through breeding with wild narrowleafed lupin to extend the range for profitable production of this legume. However, this will require knowledge on the phenology of narrow-leafed lupin and identification of the genes and mechanisms retained in wild populations that allow for successful adaption in a range of climates. The early flowering locus Ku has been strongly selected for in current varieties for drought escape. Previous research indicated that the floral integrator gene FT is the gene underlying the Ku locus. This new research will focus on the gene expression of FT including its timing, tissue-specificity and response to environmental cues including day length, cold treatment (vernalisation) and ambient growing temperature. It will also provide insight into how contrasting wild and domesticated lines respond differently to these environmental cues at the molecular level. GRDC National Region Contract 01/01/2014 Start Contract End Contact Mrs. Robyn Turner Organisation Phone 31/12/2014 Project Code UHS10662 University of Sydney +61 2 8627 1003 Email [email protected] Project Summary This project will investigate the effects of a preceding rotation crop of wheat, mustard or chickpea on the incidence of crown rot in wheat. The project will test the hypothesis that preceding mustard and chickpea rotation crops will reduce the incidence of crown rot in wheat. This research project will include both glasshouse and field experiments. Pot experiments will be grown in a glasshouse in Cobbitty and a field experiment at the Narrabri Plant Breeding Institute. The tests will also be independently validated with replicated field experiments on growers' properties around Warren, and farmers will be consulted once the final research proposal has been approved. The incidence and presistance of crown rot throughout the season will be assessed in wheat, Indian mustard and chickpea following inoculation with crown rot at sowing. The crown rot infection levels will be monitored and scored throughout the season. This includes monitoring fungal populations in the soil and scoring the crown rot infection within the wheat treatment (including white heads), until the crop reaches maturation at the end of the growing season in 2014. 229 6. Building Skills & Capacity UHS10662: UHS - Eleanor Readford (US) The effect of preceding rotation crop (wheat, mustard and chickpea) on the incidence of crown rot in wheat UHS10663: UHS - Ashley Jacobs (UWA) Economic Analysis of the Harrington Seed Destructor GRDC National Region Contract Start 22/07/2013 Contract End Contact Dr. Thea Linke Organisation Phone 25/05/2014 Project Code UHS10663 University of Western Australia 6488 1166 Email [email protected] Project Summary A new component of Integrated Weed Management (IWM) is the emerging availability of the Harrington Seed Destructor (HSD). Towed behind a harvester, the HSD is a trailer-mounted system that incorporates a high capacity cage mill to process chaff residue and destroy weed seeds. 6. Building Skills & Capacity This study intends to conduct an economic assessment of the economic worth of using the HSD as part of IWM strategies for different 230 weedy farm environments, size of cropping area and average crop yields. Various ways of utilising a HSD and sharing its ownership cost in these different weedy environments will be investigated. This study will involve updating and applying RIM (Ryegrass Integrated Management), a weed management simulation model to determine results. UHS10665: UHS - David Brunton (UT) Determining the residual effect of Roundup (glyphosate) on successful crop establishment Contract Start 01/01/2014 Contract End Contact Associate Professor. Peter Lane Organisation Phone 11/01/2014 Project Code UHS10665 University of Tasmania +61 3 6226 7180 Email [email protected] Project Summary Recent observations in certain crops, particularly poppies, throughout Tasmania have shown symptoms that many believe could potentially be residual glyphosate damage. Roundup (glyphosate as the active ingredient) is used commonly as a pre-sowing, non-residual, non-selective knockdown herbicide. Observed damage has been closely associated with lighter soil types, short plant back periods and high application rates of glyphosate, particularly evident when 1500 g.a.i or 2.7 - 3L/ha is applied. The damage witnessed is seen early on in the establishment phase and places the crop at a disadvantage with regard to achieving particular growth stages at certain time periods after planting. Subsequent chemical applications within the season are delayed and the crop is more prone to environmental factors such as waterlogging, frost and herbicide damage from other products. These factors not only push back the available growing period but also place a higher requirement upon irrigation to finish crops off when summer rainfall ceases, potentially reducing profit margins. For this research project it is hoped that by gaining a greater understanding of the way glyphosate interacts within the soil, field agronomists may be in a position to better understand the factors (if proven to be applicable) when applying glyphosate based products, pre-sowing when planting potentially susceptible crops in some soil types. 6. Building Skills & Capacity GRDC National Region 231 UHS10668: UHS - Belinda Worland (UQ) Gene expression of nitrate transporters in Sorghum bicolor (BTx623) under variable conditions of nitrate supply GRDC National Region Contract 01/01/2014 Start Contract End Contact Ms. Leanne Pooley Organisation Phone 22/11/2014 Project Code UHS10668 University Of Queensland 0754601342 Email [email protected] Project Summary Nitrate is the dominant form of nitrogen in most agricultural soils but it is also easily lost to the environment causing serious pollution problems. Nitrate transporters are responsible for the capture of nitrate from the soil and moving it throughout the plant for growth and grain production. The presence, abundance and type of nitrate transporters can determine how effectively a plant can capture and mobilise nitrogen from the soil. 6. Building Skills & Capacity This project will analyse the nitrate transporter 232 genes expressed by Sorghum plants that have been grown under different soil nitrate conditions. Advanced genetic analysis (qRTPCR) will be employed to characterise the nitrate transporter genes that are differentially active in the roots, stem and leaf tissues. This project is highly novel because Sorghum has not been characterised in this respect and existing knowledge is limited to assumptions made from work with model plants. GRDC National Region Contract 24/02/2014 Start Contract End Contact Mr. Calum Wilson Organisation Phone 17/10/2014 Project Code UHS10669 University of Tasmania 03 6233 6841 Email Calum,[email protected] Project Summary This project will provide farmers in areas to be serviced by new irrigation infrastructure in Tasmania’s midlands with quantitative data on the economic viability of irrigated wheat production. Cost-benefit analysis will be used to compare the economic returns of conventional irrigated agriculture in which wheat is a secondary crop, to irrigated farming systems in which wheat plays a more dominant role. Sensitivity analysis around production, price and input cost variables will provide insights to farmers into the threshold factors that determine the economic viability of irrigated wheat production. Leading grain producers who work closely with TIA’s Extensive Agriculture Centre will design the farming systems to be assessed, and provide relevant production, price and cost data through structured interviews and small group workshops. Where possible, the analysis will draw on existing farming systems modelling using APSIM to provide data on the productivity interactions between wheat and other crops. The analysis will focus on the economic interactions between crops in Tasmania’s diverse irrigated farming systems that are critical to the economic viability of irrigated wheat production. This knowledge will be used to draw insights into the agronomic and value chain preconditions necessary for large scale expansion of the wheat industry in Tasmania. 233 6. Building Skills & Capacity UHS10669: UHS - Danielle Button (UT) Thresholds of success - the on-farm economics of expanding irrigated grain production in Tasmania's midlands UHS10670: UHS - Emily Lamberton (US) Effect of windrow burning on water efficiency during the fallow GRDC National Region Contract Start 03/03/2014 Contract End Contact Mrs. Robyn Turner Organisation Phone 31/10/2014 Project Code UHS10670 University of Sydney 02 8627 1003 Email [email protected] Project Summary The aim of this project is to determine whether the fallow moisture efficiency is affected by the practice of windrow burning. This project will be conducted in the Central West Plains of NSW under multiple treatment levels. The first will compare the affect that windrow burning has in areas of higher rainfall (Wellington) compared to areas of lower rainfall (Nyngan). 6. Building Skills & Capacity The second treatment level will be to compare the affect that windrow burning has on fields with light stubble compared to those with high stubble loads. 234 The measurements that will be taken at each site include: gravimetric soil water content, hydraulic conductivity, soil carbon, soil nitrogen and pedological stability of soil aggregates. All measurements will be taken before burning (for a base level) and after burning (at the end of the fallow). If a significant rainfall event occurs mid-fallow it is likely a mid-fallow measurement will also be taken. Control strips will be set up for each trial that have not been burned to serve as a point of comparison. From these results, agronomists and farmers will be able to more accurately determine whether there is a greater cost or benefit of windrow burning, relating to their particular circumstances. It will promote informed farm descisions and precise practices. UHS10671: UHS - James Walter (UA) Interactions between phosphorus nutrition and plant development Contract Start 29/07/2013 Contract End Contact Miss. Chelsea Du Bois Organisation Phone 23/06/2014 Project Code UHS10671 University of Adelaide 08 8313 3716 Email [email protected] Project Summary The aim of this research is to identify if a phenologic relationship exists between phosphorus nutrition and developmental genes in wheat. The main point of interest of this project will be the relationship between differing phosphorus rates and different combinations of the VERNALIZATION1 (VRN1) gene set (the primary gene controlling vernalization), when exposed or unexposed to a vernalization treatment. This will be investigated through a series of growth room and glasshouse experiments, using near isogenic lines (in a Sunstate background) which differ in their VRN1 genotype. Early apical development, shoot/root phenes and spikelet number of these plants will be assessed in relation to varying rates of phosphorus treatment, to determine the interactions between phosphorus nutrition and the VRN1 gene. Genetic mapping of wheat has shown an association between the VRN1 gene of wheat and phosphorus use efficiency, however no research has been conducted into their relationship; allowing this project to fill a current knowledge gap in the grains industry, as well as contributing to the broader understanding of the genetic control behind phosphorus use efficiency. 6. Building Skills & Capacity GRDC National Region 235 UHS10672: UHS - Jared Murray (UWA) The biochemical mode of action for members of a new stream of potential herbicides GRDC National Region Contract Start 01/01/2014 Contract End Contact Associate Professor. Joshua Mylne Organisation Phone 18/11/2014 Project Code UHS10672 University of Western Australia 08 64884415 Email [email protected] Project Summary Herbicide resistance is threatening global food supply. The recent Global Herbicide Resistance Challenge conference noted that "no new herbicide mode of action discoveries had been made" and that there are "no new ones coming in the foreseeable future". 6. Building Skills & Capacity We have made an exciting discovery that many drugs and drug leads developed against the malarial parasite Plasmodium falciparum are also herbicidal. This is perhaps not all that surprising as for decades scientists have known that herbicides can kill malarial parasites. 236 We propose to use plant natural variation to screen for resistance and, in this way, attempt to find the biochemical targets of these drugs in plants. We will use the model plant Arabidopsis thaliana, which has rapid cycling times, a well annotated genome and a wealth of genetic resources, to screen for and dissect resistance. We have recently acquired 260 genetically diverse varieties of Arabidopsis thaliana collected from around the world as well as 400 inbred lines derived from intercrossing 19 parents that permit rapid localisation of genes underlying quantitative traits. To dissect modes of action, we also have Arabidopsis mutants resistant to herbicides isoxaben, paraquat and the imidazolinone/sulfonylurea classes of herbicides. GRDC National Region Contract 29/07/2013 Start Contract End Contact Ms. Barbara Jamieson Organisation Phone 30/05/2014 Project Code UHS10673 University of Western Australia 08 64881782 Email [email protected] Project Summary Drought has the most significant influence in limiting wheat production in Australian dry-land agriculture. To combat climate change pressures of predicted decreasing annual rainfalls and increasing seasonal temperatures, investment into development of drought tolerant wheat cultivars needs to be conducted. The key problem in innovation of new drought tolerant cultivars is the ability to efficiently select a number of genotypes to identify genetic variation for specific traits and therefore, recognize appropriate parent material for breeding programs. The crucial elements for breeders to be able to select for desirable traits requires easily identifiable and measurable characteristics. Physiological mechanisms associated with plant drought tolerance may potentially be utilised as a screening method to identify cultivars tolerant to water-limiting environments. The aim of this project is to establish the relationship between physiological mechanisms that influence plant water status and gas exchange parameters in leaves of numerous genotypes of wheat exposed to drought conditions, and evaluate if this relationship can be utilised as a reliable indicator to screen for drought tolerance. 237 6. Building Skills & Capacity UHS10673: UHS - Kyle Mart (UWA) Screening for drought tolerance in wheat: Examining leaf osmotic potential as a predictor of turgor loss point UHS10674: UHS - Lachlan Rainer (RMI) Effect of cultivar, growth region and malting conditions on the limit dextrinase activity and inhibition in Australian malting barley GRDC National Region Contract 24/02/2014 Start Contract End Contact Dr. Lisa Dias Organisation Phone 15/11/2014 Project Code UHS10674 Royal Melbourne Institute of Technology 03 9925 1751 Email [email protected] Project Summary I have shown in my current research project that cultivar and growing conditions both appear to have an effect upon the limit dextrinase activity. As part of this work, I have measured both the bound and free forms of the enzyme. 6. Building Skills & Capacity On this basis there is now strong case to extend this work to include an assessment of the effects of inhibitors of the enzyme which have been reported to be present in barley grains. The extent to which limit dextrinase is active in a brewery mash is highly dependant on the rate and extent to which the enzyme is released from its inhibitor. 238 Due to the significant economic importance of this enzyme, it is important to understand this process with the goal of optimising limit dextrinase activity. A further extension of the work will be to evaluate the effects of varying malting conditions on limit dextrinase activity and inhibition. Recent study has shown how new malting techniques can speed up the process. I would like to evaluate the effect of these new methods on limit dextrinase. UHS10675: UHS - Matthew Blanc (UQ) TALENs as a novel Sorghum bicolour transformation method Contract Start 01/01/2014 Contract End Contact Mr. Blake Chapman Organisation Phone 10/01/2014 Project Code UHS10675 University Of Queensland 0733469966 Email [email protected] Project Summary TALENs (Transcription activator-like effector nucleases) are artificial restriction enzymes that can be used to alter DNA at a gene specific level. TALENs can be engineered to have a binding domain that can specifically target one or multiple sequences within a genome. This can then be used to either create a point mutation to knock out a gene, change expression or allow insertion of a desired DNA sequence. This allows nonrandom insertion of genes, not possible with other methods widely used. TALENs are a new technology but have been shown to work in many different plants species, but as yet noone has tried in Sorghum. Sorghum is a crop grown mainly in Africa as a subsistence food due to its environmental tolerance. The problem with Sorghum is that of all major grain crops, it is the least digestible due to tightly bound starch:protein matrix. Any work that can be done to improve the digestibility will be invaluable to those who grow it. To test the effectiveness of TALENs in Sorghum, they will be used to separately upand down-regulate the expression of starchbranching enzymes in the grain of plants to assess the effect on the digestibility of the end product. 6. Building Skills & Capacity GRDC National Region 239 UHS10676: To characterise a subset of genes in Stagonospora nodorum and determine their role in causing disease GRDC Region Contract Start 01/01/2014 Contract End Contact Ms. Amelia Irby 31/10/2014 Project Code UHS10676 Organisation Phone 02 6125 9394 Email [email protected] or [email protected] Project Summary Stagonospora nodorum is the causal agent of glume blotch disease on wheat. A recent survey of wheat diseases in Australia rated glume blotch as the 3rd most important in terms of economic losses. It has now been well established that the pathogen causes infection through the secretion of pathogenicity proteins known as effectors. However there are also many other aspects to the pathogen which are critical in determining a successful infection. 6. Building Skills & Capacity Through a previous GRDC investment in the laboratory of Assoc. Prof. Peter Solomon, the infection of Stagonospora nodorum on susceptible and resistant wheat lines was 240 studied with the aim of identifying mechanisms of pathogenicity. In this approach, the Solomon laboratory identified up to 50 pathogen genes that were expressed only during infection on a susceptible host, suggesting that they are required for infection. The aim of this project is to characterise a subset of these genes and determine their role in causing disease. This will be acheicved using established techniques in the Solomon laboratory of selectively removing the genes from the Stagonospora nodorum and assessing the ability of the mutant strains to cause disease. UHS10677: UHS - Olivia Agar (US) Discovering genetic variation in pollen heat tolerance in wheat Contract Start 03/03/2014 Contract End Contact Mrs. Robyn Turner Organisation Phone 31/12/2014 Project Code UHS10677 University of Sydney +61 2 8627 1003 Email [email protected] Project Summary This research project investigates whether there is genetic variability in wheat hightemperature tolerance due to pollen traits and the mechanisms for pollen heat tolerance. This study will improve understanding of sensitivity to abiotic stress in wheat crops which will enable farmers to consider different management options such as better varietal selection. chambers at the Centre for Carbon Water and Food (CCWF) at the pollen development (meiosis) and anthesis stages and there will also be non-heated controls. A subset of four varieties (from the set of 20) with known heat tolerance and sensitivity will be grown in the greenhouse for detailed microscopy work to determine the mechanisms of pollen sensitivity and tolerance. This project will test the hypothesis that heat stress during pollen development and anthesis will reduce pollen viability and yield in wheat. This investigation will include both greenhouse and field experiments. Twenty breeding lines and varieties (with four replicates) will be grown outdoors in pots at Cobbitty and heated (35/22 deg Celcius, day/night) in heating The same set of twenty lines will also be heated in field chambers at the Narrabri Plant Breeding Institute to validate the methodology in the field. Viability of the pollen will be determined using tetrazolium viability tests, and kernel weight, kernel number and 1000kernal weight will be measured. 6. Building Skills & Capacity GRDC National Region 241 UHS10678: UHS - Scott Thompson (UWA) Does the reflectance of visible and near infrared light differentiate between frosted and non-frosted wheat? GRDC National Region Contract 29/07/2013 Start Contract End Contact Miss. Natalie Jaqals Organisation Phone 30/05/2014 Project Code UHS10678 University of Western Australia 64882203 Email [email protected] Project Summary Frost damage to cereal crops is a problem affecting the productivity of the grains industry every year. Currently there is no method to accurately quantify the extent of damage at the time of the event, except manually checking individual plants for damage. 6. Building Skills & Capacity Operational efficiency requires a tool to identify frost damage for in season management. Remote sensing provides an opportunity to detect plant stress on a broad acre scale, with the use of satellite imagery and appropriate vegetation indices. 242 To be successful, it must first be identified the reflectance response of frosted wheat plants and the index that will best display the reflectance response specific to frost damage. To achieve this wheat plants will be exposed to frost stress with the use of a frost cabinet at 2C, 0C and -2C. The treatment groups will be imaged hyperspectrally 1 day before exposure and 1, 2, 4, 8 and 16 following exposure. The reflectance data will be analysed using repeated measures ANOVA for multiple vegetation indicies. This aims to determine the point where reflectance of frost affected plants becomes statistically significant from unfrosted controls and which index is best suited to interpreting this data. UHS10679: UHS - Shona Wood (UQ) Field assessment of highly digestible sorghum starch biosynthetic pathway variants Contract Start 18/02/2014 Contract End Contact Dr. Blake Chapman Organisation Phone 31/10/2014 Project Code UHS10679 University Of Queensland (07) 3346 9966 Email [email protected] Project Summary Sorghum is a drought- and heat-adapted cereal, capable of consistent production under environmental conditions where other cereals may fail. However, in comparison to other cereals sorghum has a lower digestibility, starch being the major component of calories in grains. Pullulanase is a starch metabolic gene that is widely conserved across plants, and preferentially removes short chain carbohydrates. Previous work in the Godwin lab has identified two allele types associated with the pullulanase: PUL-RA occurs at low frequency and has been shown to improve digestibility, with no associated to deleterious pleiotropic effects. In contrast the PUL-GD occurs at high frequency and is associated with lower digestibility and pullulanase activity (Gilding et al, 2013 Nature Comm.). The effects of the allele type were shown to be heritable and occur regardless of genotypic background. This project aims to conduct a replicated field trial, containing replicates of homozygous PUL-GD, PUL-heterozygous, and homozygous PUL-RA Near Isogenic Lines (NILs). I will measure yield, yield components (seed size, seed number, seed specific density) and overall biomass of genotypes. The digestibility of each will also be measured and compared. Other important traits such as phenology, maturity, leaf number and height will also be recorded. 6. Building Skills & Capacity GRDC National Region 243 UHS10681: UHS - William Nak (UQ) Pseudomonas infection response in NSP mutants GRDC National Region Contract Start 28/01/2014 Contract End Contact Ms. Kaye Hunt Organisation Phone 31/10/2014 Project Code UHS10681 University Of Queensland (+61 7) 3365 3458 Email [email protected] Project Summary During the course of my honours project in the Batley lab I intend to utilise Arabidopsis plants that have mutations in the putative microbe signalling genes, NSP1 and NSP2. In legumes, the NSP genes encode transcription factors that are required to initiate symbiosis with rhizobia bacteria in the soil. these genes in microbe-response in the Brassicaceae family of plants, to which Arabidopsis belongs. This will involve bacterial response assays to quantify changes in disease physiology and the expression of known microbe-responsive genes in these mutants. Previously it has been shown that the loss of a receptor gene which acts upstream of the Arabidopsis NSP genes causes the plants to have increased susceptibility to various bacterial infections. This project will be the first to examine the role of NSP1 and NSP2 in microbe signalling in Brassicas, which includes important oilseed and vegetable crop species 6. Building Skills & Capacity Specifically I aim to confirm the involvement of 244 UHS10782: UHS - Bethany Radford (RMI) The effects of pre-processing on starch characteristics in cereal grain foods Contract Start 03/03/2014 Contract End Contact Dr. Lisa Dias Organisation Phone 15/11/2014 Project Code UHS10782 Royal Melbourne Institute of Technology 03 9925 1751 Project Summary Starch is a major component of grains and it is known that various changes occur at a molecular level during processing. As a result foods can become less appealing so that staling and firming occur. As instant products including rice and noodles become increasingly popular there is a need to be able to measure these changes reliably. This will lead to an enhanced understanding of what is happening and the development of strategies that can be used in breeding as well as processing operations. This project will utilise enzymatic analysis, X-ray diffraction and other advanced instrumental techniques to evaluate the effects of heating, various drying procedures and storage on starch systems. These studies will be extended and applied by investigation of rice and wheat and products prepared from these. This will allow a comparison of two types of starches having distinct characteristics in relation to the changes during heating, cooling and storage. These will be compared to the sensory changes as well as the nutritional effects as the starch becomes less digestible during storage. Email [email protected] 6. Building Skills & Capacity GRDC National Region 245 US00069: Enhancing human capacity for soil knowledge transfer and decision making for a sustainable grains industry GRDC National Region Contract Start 11/01/2013 Contract End Contact Prof. Alex McBratney Organisation Phone 31/10/2015 Project Code US00069 University of Sydney 2 8627 1130 Email [email protected] Project Summary In the 2010-11 stocktake of Australia’s current investment in soil research development & extension it was found that soil science education of new graduates and those working in industry is a major priority. In particular there is a need for a national approach to building future skills and capacity and this is an important goal because; 6. Building Skills & Capacity 246 it is likely the new skills and new information will be required to meet future challenges, for example manipulation of soil biology to improve nutrient supply to crops, there has been a significant loss of skills and capacity in recent years, especially from government agencies, and this has not been replaced by other organizations, and the demographics of Australian soil science suggests that a significant proportion of scientists are likely to retire in coming years. Simultaneously, the main needs identified by soil scientists and those needing to use soil science knowledge are; having a strong grounding in soil knowledge and skills to tackle diverse problems by integrating their knowledge of pedology, soil chemistry, soil physics and soil biology, be able to integrate this with other discipline knowledge relevant to agriculture and use this to apply relevant solutions, this should involve developed critical thinking and problem-solving skills, and have the communication and report writing skills to report the solutions they have found. This project will find out what are the core body of knowledge and skills needed by soil scientist and advisors who give advice to growers in the grains industry. This soil science core body of knowledge (CBoK) will be based on feedback from community surveys and industry workshops, which include academics, consultants, industry advisors and growers. This CBoK will be taught as part of the undergraduate curriculum in the Universities that are members of the Australian Council of Deans of Agriculture to ensure a lasting legacy for the training of soil scientists, agronomists and advisors needing core soil skills. In addition to the incorporation of the CBoK into an undergraduate curriculum the need for in-service postgraduate training will also be investigated. This will involve making sure that the soil CBoK is relevant for industry advisors after they have some in-field experience and if needed, supplementing this with any missing knowledge or skills, and developing a customizable teaching framework. It is expected that both graduates and those with in-field experience from both the curriculums will be able to use their new found soil science knowledge and skills for accreditation as a Certified Practicing Soil Scientist. The outcomes of this project will be a customizable teaching framework for the delivery in both an undergraduate, as well as, in-service training postgraduate curriculums. This will be delivering a soil science core body of knowledge (CBoK) essential for graduates and is relevant for the ongoing training of advisors in the grains industry. 247 6. Building Skills & Capacity UT00029: Primary Industry Centre for Science Education (PICSE) - Phase 3 Extension GRDC Region National Contract 07/01/2013 Start Contract End Contact Mr. Gordon Stone Organisation Phone 30/06/2014 Project Code UT00029 University of Tasmania 0408 063 229 Email [email protected] Project Summary 6. Building Skills & Capacity The Primary Industry Centre for Science Education (PICSE) is a proven national collaboration between universities, regional primary industries, national R&D Corporations, national agribusiness, regional research institutes, local community organisations, schools and State Government Departments. 248 The programs run by PICSE attract an increased supply of high-quality, young people into science-based primary industries and their supporting businesses through engagement with students during school years and early university. PICSE delivers science class activities, teachers’ professional development, teaching resources, student camps and student industry placement programs, all building strong and sustainable relationships between students and with local scientists and employers in primary industries, hence attracting students into tertiary science and increasing the number of skilled professionals in science-based primary industries. A National Office staff coordinates delivery of PICSE programs through a formal Senior Management Team (SMT). The SMT manages quality assurance, delivery on-ground & innovation, as well as stakeholder engagement and future planning. In 2012 PICSE supports and manages 9 Activity Centres across Australia (UTAS, Curtin University, USQ, GrowSmart Training (SA), Cotton, University of Adelaide and the pilot Science for Growth awards, which is an on-line version of the Science Investigation Awards. This has significantly extended our reach to cover metropolitan, regional and remote communities, along with expansion during 2013 to Charles Darwin University, Ballarat University & the Unversity of Western Sydney. The Outcomes of the PICSE programs to date have shown significant stakeholder involvement. In 2012 alone, over 6000 students, 130 teachers and members of the community and agribusinesses participated; this has led to 211 PICSE graduates going on to undertake science undergraduate degrees (2012 QualDATA evaluation). Independent evaluation has also shown that the PICSE program has provided collaborators and investors with a high rate of return on their investment. In particular, evaluation of PICSE programs has shown an evidence-based impact on raising the profile, capacity, interest and study choice by students with respect to study options for science courses and careers in the biosciences. Not only do the programs increase the understanding by students of the importance of science in Primary Industry but they also increased positive ongoing relationships with potential future employers and the University sector. During 2012/13 a PICSE MK II was developed to create efficiencies whilst building on successes to date. This proposal continues GRDCs support of capacity building and workforce development initiatives that secures the pipeline of future scientific personnel, crucial to the future of the Australian Grains Industry. GRDC Region Western Contract 01/04/2008 Start Contract End Contact Prof. Lorenzo Faraone Organisation Phone 30/08/2013 Project Code UWA00114 University of Western Australia +61-8-6488 3104 Email [email protected], [email protected] Project Summary We seek support from GRDC for 50% funding for 4.5 years for a Lectureship (Level B) in production agronomy and farming system. The expected outcomes (benefits) from the project include: 1. Capacity building in production agronomy and farming systems in WA and Australia 2. Improved production technologies for the benefit of WA grain growers and the industry 3. Impreove collaboration between ARWA partners Comprehensive annual progress report of the project will be provided to GRDC. In addition to this, we suggest that GRDC conduct a formal mid-term review of the project. Provided the position achieves the academic goals, delivers the outcomes for the industry, and generates the required income to sustain itself, the School of Plant Biology will fully fund the position beyond 4.5 years. 249 6. Building Skills & Capacity UWA00114: Capacity building in Production Agronomy and Farming System (teaching, research and postgraduate training) at UWA in partnership with GRDC