Zinc-enriched amygdalo- and hippocampo-cortical connections

Neuroscience Research 53 (2005) 57–68
www.elsevier.com/locate/neures
Zinc-enriched amygdalo- and hippocampo-cortical connections
to the inferotemporal cortices in macaque monkey
Noritaka Ichinohe *, Kathleen S. Rockland
Laboratory for Cortical Organization and Systematics, Brain Science Institute,
RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
Received 9 April 2005; accepted 3 June 2005
Available online 14 July 2005
Abstract
Synaptic zinc (Zn), a co-factor in some glutamatergic synapses, has been implicated in plasticity effects, as well as in several excitotoxic
and other pathophysiological conditions. In this study, we provide information about the distribution of Zn in inferotemporal cortex, a region
at the interface of the visual and hippocampal networks. In brief, we found a lateral to medial increase in Zn, where TEad, a unimodal visual
area, showed low levels of Zn; TEav, intermediate levels; and perirhinal cortex, a multimodal limbic area, high levels. The distribution of
parvalbumin, a calcium binding protein, showed a reverse gradient to that of Zn. The neurons of origin of the Zn+ termination were identified
by making intracortical injections sodium selenite (Na2SeO3). This substance interacts with Zn to form precipitates of ZnSe and in this form is
transported retrogradely to the soma. A mixed population of labeled neurons was visualized, which included Zn+ neurons in CA1 of the
hippocampus and in several amygdala subnuclei. In CA1, Zn+ neurons were restricted to the upper part of stratum pyramidale. Zn is thought
to contribute to activity-dependent synaptic plasticity. The specifically high level in perirhinal cortex, and its origin from neurons in CA1 and
the amygdala, may relate to cellular events involved in visual long-term memory formation.
# 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
Keywords: Parvalbumin; Gradient; Plasticity; Limbic; Theta rhythm
1. Introduction
Zinc-positive (Zn+) terminations occur widely throughout cortical areas, as has been shown in both rodent (Garrett
and Slomianka, 1992; Perez-Clausell, 1996) and primates
(Carmichael and Price, 1994; Franco-Pons et al., 2000;
Ichinohe and Rockland, 2004). In the monkey, they are
particularly dense in orbitofrontal and parahippocampal
regions, and in much of the pre-Rolandic frontal cortex. In
these and several other areas, a further specialization is seen
in the form of distinct patches or honeycomb at the border of
layers 1 and 2 (Ichinohe and Rockland, 2003, 2004).
Variations in distribution of this Zn+ system are potentially
important (1) as a marker of area or regional boundaries, and
(2) as a clue to functional specialization. That is, Zn is
believed to act in a neuromodulatory role, with significant
influence on plasticity effects. It is released in an activityand calcium-dependent fashion, and interacts with many
Abbreviations: AAA, anterior amygdaloid area; AB, accessory basal nucleus; ABmc, accessory basal nucleus, magnocellular division; ABpc, accessory
basal nucleus, parvicellular division; ACo, anterior cortical nucleus; AHi, amygdalohippocampal area; amts, anterior middle temporal sulcus; Amyg,
amygdala; Bi, basal nucleus, intermediate division; Bmc, basal nucleus, magnocellular division; Bpc, basal nucleus, parvicellular division; Ce, central nucleus;
cs, central sulcus; EC, entorhinal cortex; En, endopiriform nucleus; Hp, hippocampus; I, intercalated nuclei; L, lateral nucleus; lf, lateral fissure; Me, medial
nucleus; PAC, periamygdaloid cortex; PCo, posterior cortical nucleus; PRh, perirhinal cortex; Pi, piriform cortex; PL, paralaminar nucleus; pmts, posterior
middle temporal sulcus; Pros, prosubiculum; rhs, rhinal sulcus; sts, superior temporal sulcus; TEa, anterior inferotemporal cortex; TEad, anterior dorsal
inferotemporal cortex; TEav, anterior ventral inferotemporal cortex; TEO, temporal occipital cortex; TEm, area TEm; TEp, posterior inferotemporal cortex;
WM, white matter
* Corresponding author. Tel.: +81 48 462 1111; fax: +81 48 467 6420.
E-mail address: [email protected] (N. Ichinohe).
0168-0102/$ – see front matter # 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
doi:10.1016/j.neures.2005.06.002
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N. Ichinohe, K.S. Rockland / Neuroscience Research 53 (2005) 57–68
receptors, ion channels, and neurotrophic factors (Manzerra
et al., 2001; Kim et al., 2002; Rachline et al., 2005; Hwang
et al., 2005; see for review: Cuajungco and Lees, 1997;
Frederickson et al., 2000; Smart et al., 2004).
In this report, we provide further information about the
distribution of Zn+ terminations in inferotemporal (IT)
cortex, a region at the interface of the visual and
hippocampal networks. In particular, we report a lateral
to medial increase in Zn density, where TEad, a unimodal
visual area, shows low levels of Zn; TEav, intermediate
levels; and perirhinal cortex, a multimodal limbic area, high
levels. A related question was to identify the neurons of
origin of the Zn+ terminations. This was addressed by
making intracortical injections of sodium selenite
(Na2SeO3), a substance which interacts with Zn to form
precipitates of ZnSe and in this form is transported
retrogradely to the soma (Christensen et al., 1992;
Casanovas-Aguilar et al., 1998, 2002). Analysis of retrogradely filled neurons is restricted to the hippocampus and
amygdala in this report.
2. Materials and methods
2.1. Animals and tissue preparation
Nine adult macaque monkeys (Macaca mulatta and M.
fuscata) were used (four for visualizing Zn+ terminals and
five for sodium selenite injections). All experimental
protocols were approved by the Experimental Animal
Committee of the RIKEN Institute, and conformed to the
National Institutes of Health Guide for the Care and Use of
Laboratory Animals (NIH Publications No. 80-23; revised
1996). Every effort was made to minimize the number of
animals used and any pain or discomfort experienced.
Before perfusion, all animals were tranquilized with
ketamine (11 mg/kg, i.m.) and deeply anesthetized with
Nembutal (overdose, 75 mg/kg, i.p.).
Four monkeys were prepared for visualizing Zn+
termination, according to two perfusion methods (Danscher,
1996; Ichinohe and Rockland, 2004). (1) Two monkeys were
perfused transcardially, in sequence, with saline containing
0.1% sodium sulfide (500 ml) for 5 min, and then 0.1%
sodium sulfide and 4% paraformaldehyde in 0.1 M
phosphate buffer (PB, pH 7.3, 4 l) for 30 min. The brains
were removed from the skull, trimmed and postfixed for 12–
15 h in 4% paraformaldehyde in 0.1 M PB. Then, brains
were immersed in 0.1 M PB containing 30% sucrose until
they sank (2–3 days). (2) Two other animals received
intravenous injection of saline with 10% sodium sulfide
(200 mg/kg). Two minutes after the injection, the animals
were perfused transcardially, in sequence, with saline
containing 0.5% sodium nitrite, with 4% paraformaldehyde
in 0.1 M PB for 30 min, and chilled 0.1 M PB with 10, 20
and 30% sucrose. There was no obvious difference in quality
of staining between these two methods. All brains were
coronally sectioned by frozen microtomy (at 40–50 mm
thickness). Tissue was collected in repeating series of three
for Zn and parvalbumin (PV) histochemistry, and for cell
body staining with thionin.
2.2. Zn histochemistry
Sections were washed thoroughly with 0.1 M PB,
followed by 0.01 M PB. The IntenSE M silver Enhancement
kit (Amersham International; Little Chalfont, Bucks, UK)
was used to intensify Zn signals (Danscher et al., 1987; De
Biasi and Bendotti, 1988). A one-to-one cocktail of the
IntenSE M kit solution and 33% gum arabic solution was
used as a reagent. Development of reaction products was
monitored under a microscope and terminated by rinsing the
sections in 0.01 M PB and, subsequently, several rinses in
0.1 M PB. Selected sections were further processed for Nissl
substrate using NeuroTrace 500/525 green fluorescent Nissl
stain (Molecular Probes, Eugene, OR) according to the
company’s protocol.
In addition to Zn, these methods potentially can reveal
other metals such as copper and iron (see for review,
Danscher, 1996). The specificity for Zn was evaluated by
several means. First, EM data (Ichinohe and Rockland,
2005a) clearly show that the silver reaction product is
localized to synapses, whereas iron is reported to be
localized mainly to the nucleus of neurons and glia (Yu et al.,
2001), and copper, mainly to glia (Szerdahelyi and Kasa,
1986). Second, we carried out controls in rat brains perfused
and reacted under the same two conditions as the monkey
material. In one control, intraperitoneal injection (1000 mg/
kg) of the Zn chelating agent diethyldithiocarbamate 60 min
before sacrifice (as per Danscher, 1996) resulted in unstained
brain sections. Similarly, pretreatment of brain sections with
0.1 M HCl (Danscher, 1996) for 30 min or 15% trichloroacetic acid (TCA; Sigma, St. Louis, MO) for 5 min
(Szerdahelyi and Kasa, 1986), both known to dissolve Zn
sulfide precipitates, eliminated the staining we have
interpreted as owing to Zn. The TCA control was repeated
in monkey tissue, with the same result. A direct control, such
as staining unfixed cryostat sections with TSQ or Zinpyr-1
(Frederickson et al., 1992; Woodroofe et al., 2004) was not
carried out due to the difficulty of obtaining and handling
this tissue in primates.
2.3. Immunoperoxidase staining for PV
Sections were incubated for 1 h with 0.1 M phosphate
buffer saline (PBS, pH 7.3) containing 0.5% Triton X-100
and 5% normal goat serum (PBS-TG) at room temperature,
and then for 40–48 h at 4 8C with PBS-TG containing antiPV monoclonal mouse antibody (Swant, Bellinzona,
Switzerland; 1:50,000). After rinsing, the sections were
placed in PBS-TG containing biotinylated anti-mouse IgG
polyclonal goat antibody (Vector, Burlingame, CA; 1:200)
for 1.5 h at room temperature. Immunoreactivity was
N. Ichinohe, K.S. Rockland / Neuroscience Research 53 (2005) 57–68
visualized by ABC incubation (one drop of reagents per 7 ml
0.1 M PB, ABC Elite kits; Vector, Burlingame, CA)
followed by diaminobenzidine histochemistry with 0.03%
nickel ammonium sulfate.
2.4. Sodium selenite injection
For five of the nine monkeys, 0.5–0.7% Na2SeO3 in 0.9%
NaCl was injected into temporal cortical areas (on the left
side) in order to visualize Zn+ neurons giving rise to the Zn+
terminations. Surgery was carried out under sterile conditions after the animals were deeply anesthetized with
barbiturate anesthesia (35 mg/kg Nembutal i.v. after a
tranquilizing dose of 11 mg/kg ketamine i.m.). Cortical
areas of interest were localized by direct visualization,
subsequent to craniotomy and durotomy, in relation to sulcal
landmarks (i.e. superior temporal, and anterior or posterior
middle temporal sulci: sts, amts and pmts). Pressure
injections were made by a 10 ml Hamilton syringe in
regions corresponding to TEO, TEm, TEad, TEav, and the
border of TEav and perirhinal cortex (TEav/PRh, Fig. 3).
Total volume injected was 1.0–1.5 ml, resulting in injection
sites of 1.5–2.0 mm in diameter (Fig. 3). Sodium sulfide is
toxic, and results in a central necrotic region of variable size
(see for Fig. 3A). A post-injection survival 24 h was used.
Short survival is standard for sodium selenite (Christensen
et al., 1992; Casanovas-Aguilar et al., 1995, 1998, 2002).
Moreover, in other experiments, we verified 24 h was
adequate to visualize callosal and other long distance
connections. The animals were re-anaesthetized and then
perfused transcardially, in sequence, with saline containing
0.5% sodium nitrite, 4% paraformaldehyde in 0.1 M PB for
30 min, and chilled 0.1 M PB with 10, 20 and 30% sucrose.
The brains were removed from the skull, blocked and
immersed into 30% sucrose in 0.1 M PB. The brains were
cut serially in the coronal plane by frozen microtomy (at 40–
50 mm thickness). The IntenSE M silver Enhancement kit
(Amersham International; Little Chalfont, Bucks, UK) was
used to intensify zinc signals (see above: zinc histochemistry). A precipitate assumed to be a reaction product of
injected selenium with Zn2+ at the injection site, was
visualized within cell somata, as described elsewhere
(Christensen et al., 1992; Casanovas-Aguilar et al., 1998,
2002). Sections were further processed for Nissl substrate
using NeuroTrace 500/525 green fluorescent Nissl stain
(Molecular Probes, Eugene, OR) according to the company’s protocol.
2.5. Nomenclature
Cortical areas were identified in part by reference to
sulcal landmarks, in comparison with published maps, and
by architectonic analysis of selected histological sections
stained for cell bodies. Borders were further evaluated by
analysis of laminar and density differences in the material
stained by Zn histochemistry or immunohistochemistry for
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PV. We followed most closely: for the parahippocampal and
inferotemporal cortex, the nomenclature proposed by Yukie
et al. (1990) and Saleem and Tanaka (1996), with reference
to that of Suzuki and Amaral (2003a,b); for the amygdala,
the nomenclature of Price et al. (1987) and Amaral et al.
(1992; see also Ichinohe and Rockland, 2005a,b); and for the
hippocampal formation, that of Rosene and Van Hoesen
(1987).
In referring to Zn-enriched terminals and neurons, we
have for convenience used the shorter designation Zn+
(zinc-positive).
2.6. Data analysis
Retrogradely labeled cells were plotted using a computeraided microscope system and the Neurolucida software
package (MicroBrightfield, Williston, VT). The microscope
(E800, Nikon, Tokyo, Japan) was equipped for fluorescent
and darkfield microscopy. The outline of the section, laminar
boundaries, and the borders of the amygdaloid nuclei and
hippocampal formation were added to the plots by referring
to fluorescence Nissl. Adjacent sections Nissl-stained by
thionin were available for further confirmation of these
features.
Photomicrographs were taken with an Olympus DP50
digital camera mounted on an Olympus BX60 and SZXILLD100 microscope. Images were saved in TIFF format
and imported into Adobe Photoshop 5.5. Image brightness,
contrast and color were adjusted as necessary to reproduce
the original histological data.
3. Results
3.1. Zn+ terminal distribution in the IT cortex
Zn+ terminals show a conspicuous lateral-to-medial
increase in density, from the sts to the rhinal sulcus (rhs)
(Figs. 1 and 3A for orientation). In all areas, the middle
layers (Figs. 3C and 4) are Zn-poor. These are the main
target layers of pulvinocortical terminations (Benevento and
Rezak, 1976; Baleydier and Mauguiere, 1985), and these,
like all thalamocortical terminations, are Zn-poor. Layer 1a,
another thalamorecipient layer, is also Zn-poor in these areas
(Benevento and Rezak, 1976; Baleydier and Mauguiere,
1985). The highest density of Zn+ terminations is in
perirhinal cortex (areas 35 and 36). Here, Zn+ terminations
form two distinct bands (Figs. 1 and 2A and B). The denser
band is in the supragranular layers and comprises layers 1b,
2, and 3a,b. The second band corresponds to the
infragranular layers 5 and 6. In area 35, layer 5 appears
denser than layer 6 (Fig. 2A), but in area 36, the two layers
are indistinguishable and of comparable density (Fig. 2B).
No differences could be discerned along the AP axis.
Perirhinal cortex is characterized as having a subtle, rather
than conspicuous modularity in the uppermost layers (Fig. 2
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Fig. 1. Photomicrographs of adjacent coronal sections from the anterior part of the IT cortex, stained for Zn (A) and PV (B). Medial is to the left. Star in (A)
indicates the border between area 35 (medial) and area 36 (lateral). Zn+ terminations become increasingly dense medially. Lines in (A) mark approximate
borders. Compare (B), where PV density decreases medially, in several stages (indicated by arrowheads). For abbreviations, see list. Bar: 2 mm.
Fig. 2. Photomicrographs of sections stained for Zn. Coronal sections of the border between area 35 and entorhinal cortex (A), area 36 (B), TEav (C), TEad (D),
and TEm (E). Semitangential section of TEav, in the lower bank of the amts (F). Tangential section of perirhinal cortex through the layer 1/2 border (G). Note
reticular, honeycomb pattern in both (F) and (G). Bar: 1 mm (A–F), 500 mm (G).
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Fig. 3. Schematic brain diagram showing the location of five injection sites of sodium selenite (A). Short line indicates level of section illustrated in Fig. 2.
Caudal line indicates edge of block. Photomicrographs of coronal sections through the injection core (B–E). Letters are coded to correspond to four of the
injections in (A). The most posterior injection (F) is not illustrated. In (B–C), medial is to the right. Bar: 5 mm.
in Ichinohe and Rockland, 2004). Accordingly, patches of
Zn+ terminations at the border of layers 1 and 2 can be seen
in tangential section (Fig. 2E), but may not be easily
discernible in coronal sections (Fig. 2B).
The region immediately ventral to amts is variously
called perirhinal cortex (Suzuki and Amaral, 2003a,b) or
TEav (Yukie et al., 1990; Saleem and Tanaka, 1996). This
region shows two bands, similar to more medial cortex, but
the density is not uniform (Figs. 1A and 2C). Especially in
the supragranular layers, a very dense band in layers 1b and
2 can be distinguished from a moderately dense underlying
band consisting of layer 3a,b. In an appropriate section,
where tissue is cut tangentially (for example, in amts), we
can recognize a patchy pattern at the layer 1,2 border
(Fig. 2G).
In TEad, the region lateral to amts, the overall density is
again less, except in the supragranular layers, corresponding
to 1b and 2, and uppermost layer 3 (Figs. 1A and 2D). A
moderately dense, deeper band corresponds to layer 6. In
addition, there are two weaker bands: one corresponding
to layer 3a,b and the other to layer 5a. Layer 4 is very Znpoor.
Finally, the cortex at the lip of the sts, sometimes referred
to as TEm (Seltzer and Pandya, 1978), has an even lower
density of Zn+ terminations. This forms trilaminar pattern,
where layers 1b and 2 are moderately dense, and layers 3a,b
and 6 are only weakly dense (Fig. 2E). In TEm, as for TEad,
there is no evidence of a patchy conformation in layers 1, 2
in either coronal or tangential sections.
The cortex posterior to TEa (TEp and TEO) shows a
progressive decrease in Zn density (not illustrated).
However, a similar medial-to-lateral gradient (with greater
density near the occipitotemporal sulcus, ots) can be
observed. (The Zn distribution more anteriorly, in entorhinal
cortex, is complex, and will be described in a separate
communication; Ichinohe and Rockland, 2005b.)
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Fig. 4. Darkfield photomicrographs of coronal sections through CA1, showing Zn+ neurons retrogradely labeled by the sodium selenite injection in Fig. 1E.
(A0 ) is higher magnification, from the white box in (A). (B) and (B0 ) are merged darkfield and fluorescence Nissl images, to show more clearly the stratification
of the labeled neurons in the CA1 pyramidal layer. Bar: 1 mm (A, B), 500 mm (A0 , B0 ).
Although we are not primarily concerned with area
borders, parallel sections reacted for PV showed a reverse
gradation, generally complementary to the Zn distribution.
That is, from perirhinal cortex to TEm, there is gradual
decrease of PV density, opposite to Zn. In TEp and TEO, the
Zn dense area around the ots is PV weak. According to other
studies, it is likely that PV terminations in layers 2 and 3 are
mainly inhibitory, although these in layers 3 and 4 may be a
mix of local GABAergic and thalamocortical excitatory
terminations (Williams et al., 1992; Del Rio and DeFelipe,
1994; Melchitzky et al., 1999).
3.2. The origin of Zn+ terminals in the IT cortex:
hippocampal formation
By injecting sodium selenite into several regions of IT
cortex, we were able to investigate the origin of the Zn+
terminations. This was found to be mixed, with some
neurons nearby the injection sites, some in more distant
cortical areas, and others in the hippocampus, amygdala and
claustrum. For the sake of clarity, we will here concentrate
only on the Zn+ connections from the hippocampus and
amygdala.
After injections in TEm, TEad, or more posterior areas
(TEp/TEO), no labeled cells were apparent in the Hp; but
injections in TEav or TEav/PRh both resulted in labeled
neurons. These occurred in CA1, throughout its rostrocaudal
extent, except for the uncal portion. Neurons were
concentrated at the distal portion (close to prosubiculum)
of CA1 and, in the most densely labeled fields, extended for
2.5–3.0 mm medio-lateral. Labeled neurons had pyramidalshaped somata, and were restricted to the pyramidal cell
layer (Fig. 4). Double labeling for Zn and fluorescence Nissl
showed that Zn+ neurons selectively clustered in the upper
part of the pyramidal cell layer (Fig. 4). A few neurons
occurred in the prosubiculum, but only when the injection
involved perirhinal cortex.
3.3. The origin of Zn+ terminals in the IT cortex:
amygdaloid nuclei
All five cases resulted in retrogradely labeled Zn+
neurons in the amygdala (Figs. 4 and 5). In the basal
nucleus, more laterally situated injections tended to
produce more dorsal labeling (Figs. 5A, A0 and 6). That
is, injections in TEm and TEad showed more labeled
neurons in the magnocellular and intermediate portions,
while injections in TEav and TEav/PRh revealed more
neurons in the intermediate and parvicellular portions. The
dorsal portion of the lateral nucleus contained labeled
N. Ichinohe, K.S. Rockland / Neuroscience Research 53 (2005) 57–68
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Fig. 5. Coronal sections through the Hp with Neurolucida plotting of Zn+ retrogradely labeled neurons in CA1, after sodium selenite injection into TEav (A)
and TEav/PRh (B). Labeled neurons (filled circles) are located superficially in stratum pyramidale. Medial is to the right, and rostral at the top. Bar: 2 mm.
neurons, except after the TEm injection (Figs. 4B and 5).
In all cases, the accessory basal nucleus contained labeled
neurons (Fig. 5). Only TEav/PRh injection cases, however,
resulted in any substantial number of labeled neurons in
the periamygdaloid cortical nuclei (Figs. 6C and 7D). TEp/
TEO injection showed retrograde labeling similar to TEm.
That is, labeling was only found in the magnocellular and
intermediate portions of the basal nucleus. Labeled somata
are in general oval, triangular, or polymorphic in shape
(Fig. 6A0 ).
Fig. 6. Darkfield photomicrographs of coronal sections through the amygdala, showing Zn+ neurons retrogradely labeled by the sodium selenite injection into
TEav/PRh (see Fig. 1E). (A0 ) is higher magnification, from the white box in (A). See arrows in Fig. 7 for general location of the foci of labeled neurons. Bar:
1 mm (A), 200 mm (A0 ), 400 mm (B, C).
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Fig. 7. Coronal sections through the amygdala with Neurolucida plottings of Zn+ retrogradely labeled neurons in several subnuclei, after sodium selenite
injections into TEm (A), TEad (B), TEav (C) and TEav/PRh (D). Arrows in (D) indicate location of foci illustrated in Fig. 6. Medial is to the right, and rostral at
the top left corner. Bar: 3 mm.
4. Discussion
We have demonstrated differences in the distribution of
Zn+ terminations, such that medial and anterior temporal
areas (that is, area 36) are denser than areas situated more
laterally (TEa) or more posteriorly (TEp, TEO). By making
cortical injections of sodium selenite, we further established
that neurons in the amygdala and/or hippocampus are one
source of the Zn+ terminations. These results relate to
several major issues.
4.1. Subtypes of projection neurons to the IT cortex
One is the question of subtypes within projection neuron
populations. Evidence for subtypes of excitatory neurons
has so far been more fragmentary than that for interneurons
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(DeFelipe and Farinas, 1992; Rockland, 1998; Callaway,
2002; Tsiola et al., 2003; Markram et al., 2004); but
nevertheless, some clear differences have been identified in
dendritic morphology (Katz, 1987; Lund et al., 1994),
content of non-phosphorylated neurofilament (Hof and
Morrison, 1990), morphology of axon terminations (Ojima,
1994; Rockland, 1996), and ion channel distribution
(Whitaker et al., 2000; Ichinohe et al., 2004). Neurons that
use Zn as a co-factor in glutamatergic synapses have been
considered a distinct subgroup of cortically projecting
neurons, located mainly in layers 2, upper 3, and 6
(Casanovas-Aguilar et al., 1995, 1998, 2002; Ichinohe and
Rockland, 2003; Brown and Dyck, 2004). The present
results show that some neurons in CA1 are Zn+ and that
these tend to be segregated in the upper part of stratum
pyramidale for hippocampo-temporal projection. Intraperitoneal injection of sodium selenite shows a distinct
stratification of later-born, Zn+ neurons in the upper part
of CA1 (Slomianka, 1992).
In monkeys, there are fewer data concerning subdivisions
of CA1, and these are based mainly on connections. For
temporal areas, conventional tracers produce labeling
throughout the full depth of CA1 (Iwai and Yukie, 1988;
Blatt and Rosene, 1998; Figs. 7 and 9 in Yukie, 2000;
Insausti and Munoz, 2001). However, for prefrontal areas,
some reports describe that hippocampal projections selectively originate from the deeper parts of CA1 (Barbas and
Blatt, 1995; Cavada et al., 2000). Further work is necessary
to determine whether these deeper neurons are positive or
negative for Zn.
For the amygdala, it is more difficult to assess whether the
Zn+ neurons constitute a distinct subpopulation. The
distribution of our retrogradely labeled neurons is basically
similar to published observation based on standard retrograde tracers (Iwai and Yukie, 1987; Stefanacci et al., 1996;
Amaral et al., 2003). The proportion of Zn+ neurons may be
important. In rodents, the proportion of Zn+ neurons in the
amygdala, visualized by intraperitoneal injection of sodium
selenite, is reported as 0–70%, depending on the subnuclei
and on the antero-posterior level (Christensen and Geneser,
1995). In larger animals, however, a similar procedure would
be difficult, because of the toxicity of this compound.
4.2. Amygdalo-hippocampal interactions within IT
cortices
A second issue concerns amygdalo-hippocampal interactions. These structures are frequently considered separately, as primarily involved in emotion (amygdala; e.g.,
LeDoux, 2003) or episodic memory (hippocampus; Squire
et al., 2004). There is increasing recognition, however, of
complex and important interactions (Phelps, 2004); and the
occurrence of a Zn+ population of cortical projection
neurons from both structures adds another dimension.
Previous results on connections to temporal areas have
shown that the full complement of amygdala projections
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densely terminates in layers 1, 2, 5, and 6 (Amaral and Price,
1984), while those from CA1 project densely to layers 3 and
5 of perirhinal cortex and, less densely, to the same layers of
what some authors call TEav and TEpv (Saunders and
Rosene, 1988; Iwai and Yukie, 1988; Suzuki and Amaral,
1990; Blatt and Rosene, 1998; Zhong and Rockland, 2004).
Consistent with the wider area distribution of amygdalocortical projections, the Zn+ band in the upper layers is
relatively uniform across areas. The density of the Zn+
deeper band correlates well with the distribution of
hippocampal connections, being particularly dense in
perirhinal cortex, but presumably this deeper band reflects
a combination of connections from both the amygdala and
hippocampus, as well as other cortical sources. Further work
might address whether the Zn+ terminations target similar
neuronal populations, at different locations of the dendritic
tree, or, alternately, different populations (see Ichinohe et al.,
2003; Ichinohe and Rockland, 2004).
4.3. Functional significance of synaptic Zn in the IT
cortices
A third issue is the functional significance of the synaptic
Zn. This is the subject of considerable ongoing debate (e.g.:
Cole et al., 2001; Cuajungco and Faget, 2003); but evidence
from in vitro preparations and deprivation experiments
consistently suggests a role in activity-dependent synaptic
plasticity (Frederickson et al., 1990; Li et al., 2001; Takeda
et al., 2004). Among various possible mechanisms are:
translocation into postsynaptic components (Li et al., 2001);
interaction with NMDA receptors (Rachline et al., 2005);
biphasic effect on NMDA receptors (acute block, followed
by Src family kinase-mediated up-regulation, Manzerra
et al., 2001); or activation of Trk receptors (Hwang et al.,
2005). The influence of Zn on Trk receptors is potentially
significant in the context of up-regulation of BDNF, a
neurotrophic factor associated with TrkB receptors. In
particular, perirhinal cortex of monkeys trained to learn a
pair-association task exhibits up-regulation of BDNF
(Tokuyama et al., 2000), and it may be that activitydependent Zn release influences this phenomenon. This
possibility is supported by the laminar co-localization of
Zn+ terminations and BDNF expressing neurons, in layers 2,
3, 5, and 6 (Tokuyama et al., 2000).
Finally, one might wonder about the reverse gradients of
Zn and PV. Several previous reports (reviewed in Fujita,
2002) have identified a strong anterior–posterior (AP)
gradient from area V1 to entorhinal cortex, which parallels
the AP increase in Zn. In part, this may be related to regional
characteristics, such as degree and kind of plasticity
(Murayama et al., 1997), or degree and kind of oscillatory
rhythms. An interesting recent study of striatal neurons in
rats observed opposing gradients where dorsal/lateral
neurons, including a higher density of PV-positive
GABAergic interneurons, entrained to high-voltage spindle
oscillations (7–14 Hz), and ventral/medial, entrained to
66
N. Ichinohe, K.S. Rockland / Neuroscience Research 53 (2005) 57–68
hippocampal theta rhythm (5–9 Hz, Berke et al., 2004).
Distinctive rhythmic activity has already been characterized
in perirhinal cortex of cats (Collins et al., 1999), and further
work might investigate the repertoires of synchronized
neuronal events across these interconnected structures, and
their neural substrates.
Acknowledgements
This work was supported by the Brain Science Institute,
RIKEN and Ministry of Education, Science, Sports, and
Culture of Japan (Grant Number: 17024064). The authors
would like to thank Ms. Yoshiko Abe, Ms. Hiromi Mashiko,
and Mr. Adrian Knight for their excellent technical
assistance.
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