Document 6554797

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Application Brief No.
I
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OrganicAcidAnalysisin
WineandGrapeJuiceby
CapillaryElectrophoresis
1006
Highlights
Organic
"
Operating Conditions
acids influence the stability and flavor of wines,
s_mpI,pml_mllbn:Dilute1.0 mlof juiceorwine1o50 mlwilhHPLC
gradev,,ape_.
Add the in_rna_ stondard butyric acid, I O0 pg/ml,
1 : I and mix we_t.
When it comesto fermentinggrape juice, theyhelp producers
monitorthewine makingprocesssincetheyare good indicatorsof how far the fermentationprocesshas progressed. Currentmethodsof measuringorganic acidsare eitherexpensive
or aren't ruggedenough for routineuse.
Sy_,m:
WaersQuan_ 4000 Capillary
Elec_'ophoresis
coChinFused
silica,
75prn
X 100 cm
_,¢,.o1_:
5 mM Polassium
phlflalale, 0.5 mM OFMTM Anion-BT,
pH7
Injedlon:H_d_os_a_c
45 seconds
vow: 20kVINego,,,e
Po,.._
S_,pp¥
o._dlo= 254 rim,
0.3secondtime
constant
Capillary Electrophoresistogetherwith WatersTM NICE-Pak
TM
ChemistryPackage is a new, simple,and inexpensiveapproach to organic acid analysis. To preparea sample,you
simplydilule it and add an internalstandard. Theautopurge
featureof theWaters Quanta 4000 Capillary Electrophoresis
Systemmakesany other samplepreparationproceduresunnecessary.Themethodis linear overa broad range of co_centrations,is reproducibleand hasshowngood recoveries.
Thecost per testisa mere50¢.
o,lq,ic._a
1Ch_o,
id_
Or_i_l_,_,_,_
_/mq
2 Sulfale
3 Citricacid
127
2,645
:3,291
3oo
4 Tartaricacid
5 Mal_c
acid
6 s,._,:_,_:
ac_
7 Acetic
g
_,pho_
acid
9 Lac_c
acid
_0Intemat
standard
_
260
296
ChablisWine
10
4
2
I
7.15
min_
I
Reference
1
2
Jones,W.R., and }andlk, P.,Am. lab., Iq_O, 22{91, 51 . 64
Kenney,BF.. InternationalIonChro_uk_,.c_y Sympos_'n,SanDiego,
Coht.,Po,,e_
p_o,o_ (1¢x:_1.
© 1990 Millipo_eCorporation N42
Waters Chromatography Division, Millipore Corporation, 34 Maple Street, Milford, MA 01757 (508).478-2000
:
Ion Chromatography
PowerLine" Systems
Chemical Products
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Single keyboard control and
Stainless steel and non-metallic
systems. Innovative detectors. Linear
quantitation over a wide concentration range with single point calibration. If you need to analyze for
mona- and divalent cations, ionic
surfactants, organic acids, anions,
metals, and metal complexes, talk
to Waters.
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controlled from the keyboard of the
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Analytical to pilot plant scale
chemistries. Bulk media. Specialty
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proteins, fatty acids, carbohydrates,
organic acids, carbamate pesticide
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technology. Radial
Sample
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Robotics. From sample preparation
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Detection
lit
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ement
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II
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UV/Vis: photodiode array, fixed,
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fixed and programmable/scanning
wavelength. Waters offers the food
_7,"_,]
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From integrators to networking
Special-purpose
computers, Waters has a data
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systems
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representatives are the best in the
business. Along with Waters applications chemists, they create a
support network which guarantees
your satisfaction.
Waters
D,v,s,ooof
M_LUPORE
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Waters Chromatography Division
Millipore Corporation
34 Maple Street
Milford, MA 01757
508-478-2000
Waters offers special-purpose
systems for polymer analysis, amino
acid analysis, peptide analysis,
carbamate analysis, preparative
chromatography, LC-MS, and sugar
analysis. These systems come with
installation and training, optimized
methods, quality-tested chemistries,
and the right combination of pumps,
injectors, and detectors for reproducible analyses.
PowerLine, Maxima, Baseline, Expert and Waters are trademarks ot Millipore
© 1989 Millipore Corporation
Printed in the U.S.A.
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