PRODUCT INFORMATION TNP-BSA-Biotin Base Catalog No.: T-5055

PRODUCT INFORMATION SHEET
Base Catalog No.: T-5055
TNP-BSA-Biotin
Biotin OSu is conjugated to 2,4,6-Trinitrophenyl-Bovine Serum Albumin. This product is homologous to TNP-BSA.
Properties
Chemical Name: 2,4,6-Trinitrophenyl-Bovine Serum Albumin-Biotin
Appearance: yellow to orange powder
Absorption Maximum (Lambda Max): 348 nm
Extinction Coefficient at Lambda max: 15400/M/cm
Product Usage
Conjugation is determined at pH 7.0 by measuring TNP absorbance at 348 nm using 1.54 x 10E+04 as the extinction coefficient of
TNP and is given as a molar ratio of TNP to BSA assuming the molecular weight of BSA is 60,000. The spectral properties listed for
this product are the properties of the hapten and not of the hapten-carrier conjugate. The biotin moiety is not quantified.
Store solution in aliquots at -20°C. Protect from light.
This product can be dissolved in water or phosphate buffered saline (PBS) pH 7.2 at a concentration of 1 mg/mL. When using PBS
please refer to the formulation below.
PBS Formulation:
8 g NaCl
0.2 g KCl
0.2 g potassium di-hydrogen phosphate (KH2PO4)
1.15 g di-sodium hydrogen phosphate (Na2HPO4)
Add to 1 L of water
Storage & Handling
Storage Conditions: +5°C, desiccated
Reanalysis Period: 2 Year(s)
PRODUCT INFORMATION SHEET
Base Catalog No.: T-5055
TNP-BSA-Biotin
Product Sizes
Catalog No
Size / Scale
Catalog No
T-5055-5
Size / Scale
5 mg
Quality Control
Test Name
Specifications
Test Name
Immunochemical Conjugation Ratio to Protein
Specifications
Immunochemical Solubility
Dissolves at 1 mg/mL in PBS Buffer; solution free of particulate
References
D.M. Klinman. Cross-reactivity of IgG and IgM secreting B cells in normal and autoimmune mice. J.
Autoimmunol. Vol 7, P 1 (1994).
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Rev. 1.0