Post-Operative Care Information

ATTENTION: ANIMAL CARE STAFF
Post-Operative Care Information
- Surgical Models
Catheterizations
Customer
Date of Surgery
Harlan No.
Surgeon(s)
Quantity
Male
RESET FORM
Ship Date
Customer Requests
Female
Stock/Strain
Requested Age/Weight
DOB
Surgical Facility
202
231
237
Surgical Procedure
Catheter
Volume* (mL)
Plugged End Catheter Inside Diameter
Needle Size
(Best Fit Inside Catheter)
Jugular Vein Catheterization (JVC)*
0.05
.025”/0.6mm
22 gauge
Carotid Artery Catheterization (CAC)*
0.03
.025”/0.6mm
22 gauge
Femoral Vein Catheterization (FVC)*
0.07
.025”/0.6mm
22 gauge
Femoral Artery Catheterization (FAC)*
0.06
.025”/0.6mm
22 gauge
Portal Vein Catheterization via
Superior Mesenteric Vein (PVC)*
0.08
.025”/0.6mm
22 gauge
Bile Duct Catheterization
Closed Loop (BDCCL)
0.10
.025”/0.6mm
22 gauge
Other
Other
* Standard Harlan catheter exteriorization is a fixed 2.5cm exposed length between the shoulder blades; catheter is round-tipped, and made of
polyurethane. Dimensions apply to standard catheterization option only. Catheter volumes are approximations only.
Catheter Exteriorization Options
Options
Part No.
Volume
(mL)
PinPort™
Harness
Drug Treatment
Dosage
Ketamine/Xylazine
41.7 mg Ket + 8.3 mg Xyl/kg
200 mg Ket + 10 mg Xyl/kg
Isoflurane
To effect
Inhalation
Ketoprofen
3.0 mg/kg
5.0 mg/kg
SQ
Culex Catheter
Vascular Access
Port (VAP)
Other
Catheter Lock Solution Options
Other
Last dose of analgesia given on
Heparin-Glycerol Solution (10:90 ratio)
500 IU Heparin/1 mL final solution in Glycerol
Heparin-Saline Solution
500 IU Heparin/1 mL final solution in Saline
Other
Dietary Supplementation
Standard diet ad libitum
Standard water ad libitum
Other
IM
IM
Heparin/Glycerol solution supplied by: http://www.sai-infusion.com/products/
catheter-locking-solutions
Standard Lock Solution: Heparin-Glycerol (50:50 ratio)
500 IU Heparin/1 mL final solution in Glycerol
Route
Notes
IP
IP
SQ
ATTENTION: ANIMAL CARE STAFF
Harlan Surgically-Modified Animals:
Catheter Maintenance Recommendations
1.
2.
3.
4.
5.
Individually house animals with exteriorized catheters.
Provide animals with food and water ad libitum.
Maintain animals in a clean, stable environment.
Closely examine animals’ incision(s) a minimum of 72 hours after receipt.
Incision sutures or wound clips should remain for a minimum of seven days postsurgery. Reapply new sutures or wound clips on animals kept longer than seven
days post-surgery, or as needed.
6. Continue aseptic catheter maintenance to preserve patency, per the
recommendations.
7. Immediately prior to shipping, catheters are evaluated by our surgical staff for
integrity and complete patency.
8. Clients must notify Harlan Laboratories within 24 hours of delivery for credit/
replacement of animals exhibiting abnormal behavior or moribundity or deceased
on arrival.
Bile Duct Catheterization:
Bile Duct Catheterization Post-Operative Care Instructions
Materials Needed Per Catheter for Evaluating Patency
• Trained personnel or commercial animal restraint device
• Locking, non-traumatizing forceps or hemostatic clamp
• One 22/23 gauge luer stub adapter
• One sterile 1 mL syringe
• Collection tube for bile
• 70% isopropyl alcohol
• Iodine solution
• Sterile gauze sponges
• Sterile saline for injection
• A second sterile stainless steel catheter coupler
Procedure for Evaluating Bile Duct Catheter Patency
1. For accurate and safe handling of the animal, two trained people or a commercial
animal restraint device should be used.
2. Catheter patency checks should be performed immediately upon animal arrival and
every 24 hours thereafter.
3. Catheters should be examined for signs of leakage immediately upon arrival and
every 24 hours thereafter.
4. Bile flow is from the animal’s left to the right.
5. Wipe the ends of the catheter around the coupler with iodine solution-soaked
gauze sponge, followed by a wipe of a 70% isopropyl alcohol-soaked gauze
sponge. Discard sponges.
6. Clamp the right side of the catheter loop. Remove the coupler and discard. Bile
should flow from the animal’s left side catheter.
7. Insert an adapter on a syringe filled with sterile saline into the right side catheter.
Remove the clamp.
8. Slowly inject 0.10 mL of saline into the duodenal end (right side). Re-clamp this
catheter end before removing the needle/syringe. Discard needle/syringe.
9. Following patency check, the catheter loop (and flow of bile to the intestine) is
re-established by joining the two pieces of tubing over a sterile catheter coupler.
Ensure there are no air bubbles at the inserted coupler end in the catheter, as
bubbles can impede the natural flow of the bile.
Vascular Catheterizations:
Vascular Catheterization Post-Operative Care Instructions
Depending on the customer defined surgical model, complete heparin maintenance
and aseptic catheter flushing as specified below:
I. Standard Option – Fixed; 2.5cm Exteriorized
• Complete heparin maintenance following two to four days acclimation.
• Evaluate patency every five to seven days thereafter.
II. PinPort
• Complete heparin maintenance following three to four days acclimation.
• Evaluate patency every seven days thereafter.
• Follow the specific flushing instructions for PinPorted animals on the sheet
provided with your order “Post-Operative Care Information-CatheterizationsPinPort Maintenance.”
III. Harnessed
• Complete heparin maintenance following two to three days acclimation.
• Evaluate patency every two to three days thereafter.
• Maintain positive pressure on syringe plunger when removing the syringe from
the harness.
IV. Culex Catheter
• Complete catheter heparin maintenance upon arrival.
• Evaluate patency every three to four days thereafter.
V. Vascular Access Port (VAP) • Complete catheter heparin maintenance upon client receipt.
• Evaluate patency every three to four days thereafter.
• Maintain positive pressure on syringe plunger when removing the syringe from
the port.
• Note: Dermal application of antibacterial moisturizing cream to the VAP access
site is recommended after each access.
Materials Needed Per Catheter for Evaluating Patency
• Trained personnel or commercial animal restraint device
• Two locking, non-traumatizing forceps or hemostatic clamps
• Three 22/23 gauge luer stub adapters (for Std. Option), OR non-coring needles
(Huber for VAPs; SN22 for buttons & harnesses) OR three 19 gauge luer stub
adapters (for Culex catheters)
• Three sterile 1 mL syringes
• Sterile locking solution (Standard 500 IU/mL Heparin/Glycerol Locking Solution
supplied by SAI Applications, Inc.)
• Sterile saline for injection
• 70% isopropyl alcohol
• Iodine solution
• Sterile gauze sponges
• A second sterile stainless steel catheter plug
Procedure for Evaluating Vascular Catheter Patency
1. For accurate and safe handling of the animal, two trained people or a commercial
animal restraint device should be used.
2. Wipe the catheter plugged end/harness port/skin over VAP with iodine solutionsoaked gauze sponge, followed by a wipe of a 70% isopropyl alcohol-soaked gauze
sponge. Discard sponges.
3. When clamping the catheter, use non-traumatizing forceps or hemostats; clamp
catheter on the tubing, proximal to the stainless steel plug/harness. Using a second
pair of locking forceps or hemostats, with gentle, steady force remove the stainless
steel plug from the catheter. Discard plug.
4. Insert the luer stub adapter/non-coring needle on an empty syringe into the
catheter tip (or port) and remove the clamp.
5. Withdraw lock solution. Collect at least 0.10 mL of blood into syringe to ensure all
locking solution is aspirated from the catheter.
6. Reclamp the catheter and remove the adapter/needle and syringe. Discard.
7. Insert a different adapter/non-coring needle on another syringe containing sterile
saline into the catheter and remove the clamp from the catheter.
8. Slowly inject 0.20 mL sterile saline into standard and Culex catheters, or 0.30 mL
saline into harnessed and VAP implants. Reclamp the catheter.
9. Remove the adapter/needle and syringe and discard. Insert a different adapter/noncoring needle on a third syringe containing catheter locking solution.
10.Remove the clamp from the catheter and inject the lock solution into the catheter
with the pre-determined volume as indicated on the table on previous page.
11.Clamp the catheter then remove the adapter/needle and syringe and discard. Insert
three-fourths of the length of a second sterile stainless steel plug into the end of
the catheter. Remove the clamp.
www.harlan.com | Corporate Headquarters: 8520 Allison Pointe Blvd. Suite 400, Indianapolis, IN 46250
Harlan, Harlan Laboratories, and the Harlan logo are registered trademarks of Harlan Laboratories, Inc.
PinPort is a trademark of Instech Laboratories, Inc.
© 2015 Harlan Laboratories, Inc.
RMS0215-US-EN-02-PS-296