here - Eurobiofilms 2015
Transcription
here - Eurobiofilms 2015
TUESDAY, 23 JUNE 2015 Time 09:00 -16:00 W01 – Educational Workshop Growth and imaging techniques for laboratory and clinical biofilm research Directors: Paul Stoodley, (USA) & Claus Sternberg, (Denmark) W02 – Educational Workshop Experimental biofilm infection in animals Directors: Thomas Bjarnsholt, (Denmark) Biofilm laboratory growth systems - an overview Paul Stoodley, (The Ohio State University, Department of Microbial Infection and Immunity, Columbus, OH, USA) Working with clinical and animal specimen Thomas Bjarnsholt, (University of Copenhagen, Faculty of Health Sciences, Department of International Health, Immunology & Microbiology, Copenhagen, Denmark) Biofilm flow cells - principles and assembly (lecture and hands on) Paul Stoodley, (USA) Staining biofilms for imaging - live/dead, PNA FISH (lecture and hands on mock demo) Kasper Nørskov Kragh, (University of Copenhagen, Department of Systems Biology, Copenhagen, Denmark) Rendering confocal biofilm images (Imaris lecture and hands on) Claus Sternberg, (Technical University of Denmark, Department of Systems Biology Lyngby, Denmark) Techniques for quantifying biofilm for confocal images (COMSTAT - lecture and hands on) Claus Sternberg,(Denmark) Panel Discussion From in vitro to in vivo – what to consider Thomas Bjarnsholt, (University of Copenhagen, Faculty of Health Sciences, Department of International Health, Immunology & Microbiology, Copenhagen, Denmark) Animal experiments in general – legislation and experimental designs Claus Moser, (Rigshospitalet, Department of Clinical Microbiology, Copenhagen, Denmark) Evaluation of tolerance and resistance to antibiotics in animal experiments Oana Ciofu, (ISIM, University of Copenhagen, Faculty of Health and Medical Sciences, Copenhagen, Denmark) Evaluation of inflammation and immune response in animal experiments Peter Østrup Jensen, (Rigshospitalet, Department of Clinical Microbiology, Copenhagen, Denmark) Anti-biofilm vaccine studies in animals Mark Shirtliff, (University of Maryland, Department of Microbial Pathogenesis, Baltimore, MD, USA) Transcriptomics and Tn-Seq in animal models Kendra Rumbaugh, (Texas Tech University Health Sciences Center - TTUHSC, School of Medicine, Department of Surgery - The Rumbaugh Lab, Lubbock, TX, USA) Lung and chronic wound models Claus Moser, (Denmark) Peritoneal implant and filler models Thomas Bjarnsholt, (Denmark) Osteomyelitis and dual infection models Mark Shirtliff, (USA) Time 16:00 - 16:30 16:30 - 17:00 Participants will learn the principles behind the use of various experimental systems to grow and image biofilms as well as techniques to image biofilms on clinical and ex vivo animal samples. There will be hands on demonstrations of how to assemble a basic flow cell system, perform PNA FISH and live / dead viability staining and how to best present and quantify your confocal images using Imaris and COMSTAT imaging software. There will be plenty of opportunity for interactive discussion between participants and workshop faculty during the workshop. Auditorium 01 Opening Ceremony Opening Lecture 17:00 - The Pel polysaccharide is a positively charged exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix Matthew R Parsek, (University of Washington, Department of Microbiology, Seattle, WA, USA) Opening Reception Time 09:00 - 10:00 WEDNESDAY, 24 JUNE 2015 Auditorium 01 KL01 - Keynote Lectures Insights into the function of microbial dark matters in environmental biofilms Per Halkjær Nielsen, (Aalborg University, Department of Chemistry and Bioscience - Section for Biotech, Aalborg, Denmark) 10:00 - 10:30 Time 10:30 - 12:30 12:30 - 13:00 13:00 - 15:00 15:00 - 15:30 15:30 - 17:30 19:00 - Title to be determined Andrej Trampuz (Charité - University Medicine Berlin, Center for Musculoskeletal Surgery, Berlin, Germany) Coffee Break Auditorium 01 Auditorium 02 S01 - Methods of detection & diagnosis of S02 - Mixed species fungal & bacterial biofilm biofilm infections communities Chairs: Luanne Hall-Stoodley,(USA) & Thomas Chairs: Bastiaan Krom, (The Netherlands) & Thomas Bjarnsholt, (Denmark) Thomas Neu, (Germany) Methods of detection and diagnosis of biofilm infections Luanne Hall-Stoodley,( The Ohio State University College of Medicine, Department of Microbial Infection and Immunity, Columbus, OH, USA) Fungus-bacterium interactions in the healthy oral cavity Bastiaan Krom, (Academic Centre for Dentistry Amsterdam (ACTA), Department of Preventive Dentistry, Amsterdam, The Netherlands) Old and new microbiological strategies to diagnose prosthetic joint infection José Luis del Pozo, (University of Navarra, Department of Clinical Microbiology, Division of Infectious Diseases, Pamplona, Spain) Lunch Break Poster Session I Coffee Break S03 - Treatment of biofilm infections Chairs: Antonio Oliver,(Spain) & Craig Willimas, (UK) Mixed species fungal & bacterial biofilm communities Thomas Neu, (Helmholtz Center for Environmental Research - UFZ, Department River Ecology, Magdeburg, Germany) Differential effect of antimicrobial drug combinations on Pseudomonas aeruginosa and Aspergillus fumigatus mixed microbial biofilm Jose Vazquez,(Georgia Regents University, Department of Infectious Diseases, Augusta, GA, USA) Social interactions in staphylococcal biofilms: metabolic changes in mixed S. aureus - S. epidermidis biofilms and the role of QS in antibiotic tolerance Tom Coenye, (Ghent University, Laboratory of Pharmaceutical Microbiology, Ghent, Belgium) S04 - Social interactions in biofilms Chairs: Tom Coenye, (Belgium) & Steve Diggle, (UK) Diagnosis and treatment of fungal biofilm The biofilm polysaccharide PSL is a social but infection non-cheatable trait in Pseudomonas aeruginosa Craig Williams, (University of West Scotland & Steve Diggle, (University of Nottingham, School of University Hospital Crosshouse, Institute of Life Sciences, Nottingham, UK) Healthcare Associated Infection, Paisley, UK) Organ and Children Choir Concert at the Petrov Cathedral Time 09:00 - 10:00 THURSDAY, 25 JUNE 2015 Auditorium 01 KL02 - Keynote Lectures Biofilm slime by design: viscoelastic yielding as a virulence factor for survival in chronic infections Paul Stoodley, (The Ohio State University, Department of Microbial Infection and Immunity, Columbus, OH, USA) 10:00 - 10:30 Time 10:30 - 12:30 12:30 - 13:00 13:00 - 15:00 15:00 - 15:30 15:30 - 17:30 Looking for new perspectives to fight bacterial biofilm infections - identification and use of biofilmspecific functions Jean-Marc Ghigo, (Pasteur Institute, Department of Genetics of Biofilms, Paris, France) Coffee Break Auditorium 01 Auditorium 02 S05 - Host responses to biofilms S06 - Biomechanics in biofilm and infections Chairs: Mark Shirtliff, (USA) & Niels Høiby, Chairs: Phil Stewart, (USA) & Henk Busscher, (Denmark) (The Netherlands) The host response as an accomplice to mono and multi-species biofilm infections Mark Shirtliff, (University of Maryland, Department of Microbial Pathogenesis, Baltimore, MD, USA) Mechanics of biofilm detachment in flowing fluids Phil Stewart, (Montana State University, Center for Biofilm Engineering, Bozeman, MT, USA) A taste for biofilms: how phagocytic cells recognise biofilms Gertrud M Hänsch, (University of Heidelberg, Department of Immunology, Heidelberg, Germany) From bacterial vibration spectroscopy on adhering bacteria to visco-elasticity of biofilms Henk Busscher, (The Kolff Institute at the University Medical Center Groningen (UMCG), Department of Biomedical Engineering, Groningen, The Netherlands) Lunch Break Poster Session II Coffee Break S07 - Resistance in biofilms & novel approaches to treatment Chairs: Gianfranco Donelli,(Italy) & Helena Bujdakova, (Slovak Republic) S08 - Modelling of biofilms & regulation of biofilm development Chairs: David Stopar, (Slovenia) & Kendra Rumbaugh, (USA) Rationally designing nanomedicines for the eradication of pulmonary biofilm infections Katrien Forier,( Ghent University, Department of Clinical Chemistry, Microbiology and Immunology, Ghent, Belgium) Gradual transition into a floating biofilm David Stopar, (University of Ljubljana, Department of Food Science and Technology, Ljubljana, Slovenia) Photodynamic inactivation - a possible alternative in eradication of resistant microbial biofilms Helena Bujdakova, (Comenius University In Bratislava, Faculty of Natural Sciences Department of Microbiology and Virology, Bratislava, Slovak Republic) Modeling polymicrobial wound infections Kendra Rumbaugh, (Texas Tech University Health Sciences Center - TTUHSC, School of Medicine, Department of Surgery - The Rumbaugh Lab, Lubbock, TX, USA) Time 09:00 - 11:00 FRIDAY, 26 JUNE 2015 Auditorium 01 Auditorium 02 S09 - Probiotics and new natural & synthetic S10 - Horizontal gene transfer, evolution & anti-biofilm compounds heterogeneity in microbial biofilms Chairs: Christine Imbert, (France) & Claudia Chairs: Alex O'Neill, (UK) & Mette Burmølle, Vuotto, (Italy) (Denmark) Probiotics and new natural and synthetic antibiofilm compounds Elizabeth Presterl, (Medical University of Vienna, Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Vienna, Austria) 11:00 - 11:30 Time 11:30 - 13:30 Accelerated evolution of antibiotic resistance in staphylococcal biofilms Alex O'Neill, (University of Leeds, School of Molecular and Cellular Biology (SMCB), Leeds, UK) Probiotics: a potential tool to counteract biofilm- Title to be determined Mette Burmølle, (University of Copenhagen, based infections Claudia Vuotto, (IRCCS Fondazione Santa Lucia, Department of Biology, Copenhagen, Denmark) Microbial Biofilm Laboratory, Rome, Italy) Coffee Break Auditorium 01 S11 - Environmental biofilms and human health Chairs: Andrew McBain, (UK) & Martin Rulik, (Czech Republic) Microbicide-exposure and antimicrobial resistance in biofilms in the domestic environment Andrew McBain, (University of Manchester, Manchester Pharmacy School, Manchester, UK) 13:30 - Bacterial - Trojan Horse interactions: the breakthrough of the new approaches to their study Katarina Trnková, (University of Matej Bel, Faculty of Natural Sciences, Department of The Environment, Banská Bystrica, Slovak Republic) Closing Ceremony Poster Awards