here - College of Sciences - University of Nevada, Las Vegas

 59th Annual Wind River Conference on Prokaryotic Biology June 10‐14, 2015 Aspen Lodge (Dao House) Estes Park Colorado USA 59th Annual Wind River Conference, June 10‐14, 2015 WELCOME TO THE 59th ANNUAL WIND RIVER CONFERENCE ON PROKARYOTIC BIOLOGY 2015 Wind River Executive Committee: Dr. Ron Yasbin, UNLV to University of Missouri—St. Louis Dr. Uldis Streips, University of Louisville Medical School Dr. John Iandolo, University of Oklahoma Health Sciences Center Dr. Cristina Bongiorni, Danisco Dr. Diana Downs, University of Georgia Dr. Helen Wing, University of Nevada, Las Vegas Dr. Jorge Escalante, University of Georgia Dr. Charlie Moran, Emory University Dr. Rifka Rudner, Hunter College Dr. Don G. Ennis, University of Louisiana, Lafayette Dr. Mario Pedraza‐Reyes, University of Guanajuato Dr. Eduardo Robleto, University of Nevada, Las Vegas Dr. Corrie Detweiler, University of Colorado Boulder Dr. R. Martin (Marty), East Caroline University Scientific program chairs: Dr. Alexander R. Horswill, University of Iowa Contributions from the following sponsors helped make this year’s conference possible and are gratefully acknowledged: National Science Foundation Monserate Biotechnology Group Sharklet Biotechnologies, Inc. American Society for Microbiology UNLV, School of Life Sciences College of Arts and Sciences, University of Missouri—St. Louis Dept of Microbiology, University of Iowa Funding provided by the National Science Foundation and the American Society for Microbiology allows us to support the travel of graduate students and post‐doctoral fellows to the conference. Special acknowledgement: Special thanks are due to Timothy Fishburn at UNLV for expert administrative assistance. 59th Annual Wind River Conference, June 10 ‐ 14, 2015 2
THE WIND RIVER HALL OF FAME PAST AND PRESENT PLENARY SPEAKERS 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 Dr. Victor Torres Dr. Nancy Freitag Dr. David Weiss Dr. Franz Narberhaus Dr. Michael Gilmore Dr. Gisela Storz Dr. Ferran Garcia‐Pichel Dr. Barbara Funnell Dr. Nina Salama Dr. Shelley Payne Dr. Renee Tsolis Dr. Ralph Isberg Dr. David Rudner Dr. Esther Angert Dr. Mark Gomelsky Dr. Dennis A. Bazylinski Dr. James Bardwell Dr. Sean Crosson Dr. Jo Handelsman Dr. R. Martin Roop, II Dr. Peter Christie Dr. Richard Gourse Dr. Dan Kearns Dr. Rolf Thauer Dr. Jim Imlay, Dr. Andy Camilli Dr. Jade Wang Dr. Norman Pace Dr. Roberto Kolter, Dr. Graham Hatfull, Dr. Roberto Belas, Dr. William Jacobs, Dr. Jared Leadbetter, Dr. Tina Henkin, Dr. Harry Mobley, Dr. Lynn Zechiedrich, Dr. David Figurski, Dr. Karl E. Klose, Dr. John Helmann, Dr. Victor Dirita, New York University University of Illinois‐Chicago Emory University Ruhr University Harvard Medical School National Institutes of Health Arizona State University University of Toronto Fred Hutchinson Cancer Institute University of Texas at Austin University of California Davis Tufts University Harvard Medical School Cornell University University of Wyoming University of Nevada, Las Vegas University of Michigan University of Chicago Yale University East Carolina University University of Texas‐Houston Medical School University of Wisconsin University of Indiana Max Planck Institute for Terrestrial Microbiology, Marburg University of Illinois at Urbana‐Champaign Tufts University, Howard Hughes Medical Institute Baylor College of Medicine University of Colorado at Boulder Harvard Medical School University of Pittsburgh University of Maryland Albert Einstein College of Medicine California Institute of Technology Ohio State University University of Michigan Medical School Baylor College of Medicine Columbia University Medical Center University of Texas‐San Antonio Cornell University University of Michigan School of Medicine 59th Annual Wind River Conference, June 10 ‐ 14, 2015 3
2005 2004 2003 2002 2001 2000 1999 1998 1997 University of Texas Houston Medical School Dartmouth Medical School California Institute of Technology Princeton University University of British Columbia State University of New York‐Buffalo University of Texas Houston Medical School Yale University School of Medicine Rockefeller University University of Wisconsin Princeton University Northwestern University University of Illinois‐Urbana Purdue University Brookhaven National Laboratory University of Alabama‐Birmingham Lilly Research Laboratories Tufts University Princeton University Stanford University Stanford University Max‐Planck Institute for Molecular Genetics Texas A&M University University of Illinois Montana State University University of North Texas Health Science Center University of Michigan University of California at Los Angeles University of Pittsburgh Bacteriophage Institute University of Washington Washington University School of Medicine Baylor College of Medicine Dartmouth College University of Iowa University of Texas Houston Medical School University of Washington National Science Foundation Texas A&M University Dr. Samuel Kaplan, Dr. George O’Toole, Dr. Dianne Newman, Dr. Tom Silhavy, Dr. Curtis Suttle, Dr. Paul Gollnick, Dr. Heidi Kaplan, Dr. Jorge Galán, Dr. Vincent Fischetti, Dr. Robert Landick, Dr. Bonnie Bassler, Dr. Neil Welker, Dr. Stephen Farrand, Dr. Barry Wanner, Dr. Sanford A. Lacks, Dr. Janet Yother, Dr. Malcolm Winkler, Dr. Kim Lewis, Dr. Austin Newton, Dr. Julie Theriot, Dr. Dale Kaiser, Dr. Thomas A. Trautner, Dr. Ryland F. Young, Dr. Abigail A. Salyers, Dr. John W. Costerton, Dr. Tony Romeo, Dr. Frederick Neidhardt, Dr. Olaf Schneewind, Dr. Roger W. Hendrix, Dr. Eugene Nester, Dr. Scott Hultgren, Dr. Susan M. Rosenberg, Dr. Mary Lou Guerinot, Dr. Peter Greenberg, Dr. Malcolm Winkler, Dr. John Baross, Dr. Philip Harriman, Dr. Susan Golden, 59th Annual Wind River Conference, June 10 ‐ 14, 2015 4
Wednesday, June 10, 2015 3:00‐6:00 PM 4:00‐6:30 PM 6:30‐8:00 PM 8:00‐9:00 PM Registration Welcome Reception (Lobby of main lodge) Dinner & Opening remarks Cas9‐mediated RNA targeting in bacterial physiology and eukaryotic cells David Weiss Emory University 9:00PM‐ Happy Hour at the lounge and bar Thursday, June 11, 2015 7:00‐8:00 AM 8:10–8:30 AM 8:35‐8:50 AM 8:55‐9:10 AM 9:15‐9:30 AM 9:35‐9:55 AM Breakfast (Great Room Dining Room) (Session A moderators Corey Parlet and Rodolfo Urbano) Graded CodY activity prioritizes metabolism and virulence gene expression in Staphylococcus aureus Shaun Brinsmade Georgetown University, Washington DC, USA Exploring links between metabolism and cytotoxin regulation in Staphylococcus aureus Elizabeth Ohneck New York University, New York, NY, USA The Positive (and Negative) Effects of Exogenous Fatty Acids on Enterococcus faecalis Holly Saito University of Tennessee, Knoxville, TN, USA The impact of dietary metals on the gut microbiome and Clostridium difficile infection Joseph Zackular Vanderbilt University, Nashville, TN, USA Surface microtopography limits bacterial biofilm: a novel approach to decreasing device associated biofilm infections Ryan Mettetal Sharklet Technologies, Inc, Aurora, CO, USA 59th Annual Wind River Conference, June 10 ‐ 14, 2015 5
10:00‐10:20 AM 10:20‐10:40 AM 10:45‐11:00 AM 11:05‐11:20 AM 11:25‐11:40 AM 11:45‐12:00 PM 12:05‐12:20 PM 12:30‐2:00 PM 2:00‐4:30 PM 4:30‐6:30 PM 6:30‐7:30 PM 8:00‐9:00 PM Coffee Break (Session B moderators Devon Marking and William Broach) The tumor‐like nature of a Staphylococcus aureus biofilm Ken Bayles Nebraska Medical Center, Omaha, NE, USA The importance of GL‐eDNA binding for biofilm formation in Staphylococcus aureus
Inês R. Grilo Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa Identification of novel S. aureus biofilm associated proteins with neutrophil killing function Mohini Bhattacharya The Ohio State University, Columbus, OH, USA Hyaluronan is an important component of the extracellular matrix of Staphylocccus aureus biofilm infections Carolyn Ibberson University of Iowa, Iowa City, IA, USA Regulation of clumping in Staphylococcus aureus Heidi Crosby University of Iowa, Iowa City, IA, USA Minimal peptidoglycan turnover in WT and PG hydrolase mutants of Streptococcus pneumoniae D39 growing planktonically and in host‐relevant biofilms Michael Boersma Indiana University, Bloomington, IN, USA Lunch (Great Room Dining Room) Free Time & Poster Set‐up Posters & Happy Hour (Odd number posters) Dinner (Great Room Dining Room Convergence of immune suppression and nutrient acquisition: the story of Staphylococcus aureus bi‐component leukocidins Victor Torres New York University 9:00 ‐ Happy Hour at the lounge and bar 59th Annual Wind River Conference, June 10 ‐ 14, 2015 6
Friday, June 12, 2015 7:00‐8:00 AM 8:10–8:30 AM 8:35‐8:50 AM 8:55‐9:10 AM 9:15‐9:30 AM 9:35‐9:50 AM 9:55‐10:20 AM 10:20‐10:40 AM 10:45‐11:05 AM 11:10‐11:25 AM Breakfast (Great Room Dining Room) (Session C moderators Natasha Weatherspoon‐Griffin and Loni Townsley) Regulation of Shigella virulence gene expression by RNA thermometers Erin Murphy Ohio University, Athens, OH, USA The iron‐ and temperature‐dependent regulation of Shigella heme uptake gene shuT via its 5’‐untranslated region Yahan Wei Ohio University, Athens, OH, USA Pursuit of the mechanism of virulence gene expression by VirB in Shigella flexneri Michael Picker UNLV, Las Vegas, NV, USA The 31 amino acid YneM Protein regulates phosphate and magnesium availability in Escherichia coli Hanbo Wang NIHCD, Bethesda, MD, USA Regulation of the ZorO toxin: role of the 5’ UTR Elizabeth Fozo University of Tennessee, Knoxville, TN, USA Coffee Break (Session D moderators Megan Fris and Nicole Inniss) Iron regulation of antimicrobial production by Pseudomonas aeruginosa Amanda G. Oglesby‐Sherrouse University of Maryland, Baltimore, MD, USA A novel family of genus‐specific putative regulatory RNAs in Bartonella henselae Ronan Carroll Ohio University, Athens, OH, USA VtlR: A LysR regulator that helps Brucella deal with a stressful situation Lauren Sheehan Virginia Tech, Blacksburg, VA, USA 59th Annual Wind River Conference, June 10 ‐ 14, 2015 7
11:30 ‐ 11:45 AM 11:50‐12:05 PM 12:10‐12:25 PM 12:30‐2:00 PM 2:00‐4:30 PM 4:30‐6:30 PM 6:30‐7:30 PM 8:00‐9:00 PM 9:00 ‐ Identification and characterization of three small proteins whose production is controlled by the virulence‐associated regulator, VtlR, in Brucella abortus James Brudnick Virginia Tech, Blacksburg, VA, USA Spx‐dependent transcriptional activation of divergent ypjC and ypjD (bacillithiol biosynthesis) operons: absence of Spx redox control Cierra Birch Oregon Health and Science University, Portland, OR, USA Does the Brucella MucR directly regulate the expression of the genes encoding the ferrous iron specific transporter FtrABCD in response to acidic pH?
Ahmed Elhassanny East Carolina University, Greenville, NC, USA Lunch (Great Room Dining Room) Free Time Posters Session and Happy Hour (Even number posters) Dinner (Great Room Dining Room) THE NEIL WELKER MEMORIAL PLENARY ADDRESS Introduction by Uldis StreIps Towards the global RNA structurome of Yersinia pseudotuberculosis Franz Narberhaus Ruhr University Bochum, Germany Happy Hour at the lounge and bar Saturday, June 13, 2015 7:00‐8:00 AM 8:10–8:30 AM Breakfast (Great Room Dining Room) (Session E moderators Logan Bulock and Jacob Choby) A cell pole organizing protein in Agrobacterium tumefaciens Grant Bowman University of Wyoming, Laramie, WY 59th Annual Wind River Conference, June 10 ‐ 14, 2015 8
8:35‐8:50 AM 8:55‐9:10 AM 9:15‐9:30 AM 9:35‐9:50 AM 9:55‐10:20 AM 10:20‐10:40 AM 10:45‐11:05 AM 11:10‐11:25 AM 11:30‐11:45 AM Polar Organizing Protein Z is a network‐forming hub that directly interacts with multiple binding partners Josh Holmes University of Wyoming, Laramie, WY Primary sigma factor mutations bypass comW requirement during competence for genetic transformation in Streptococcus pneumoniae Yanina Tovpeko University of Illinois‐Chicago, Chicago, IL, USA Identification of a New Lytic Transglycosylase (MltG) Involved in Peripheral Peptidoglycan Synthesis in Streptococcus pneumoniae D39 Ho‐Ching Tiffany Tsui Indiana University, Bloomington, IN, USA Proteolysis of SwrA by the LonA protease is mediated by the C‐terminal region of SwrA Anna Bree Indiana University, Bloomington, IN, USA Coffee Break (Session F moderators Heidi Crosby and Carolyn Ibberson) Exploring the expression and function of alternative oxidase in bacteria Ann Dunn University of Oklahoma, Norman OK Hcp, the Type Six Secretion System needle, is an Acinetobacter baumannii virulence factor Dan Zurawski Walter Reed Army Institute of Research, Silver Spring, MD, USA Determining the effects of Pseudomonas aeruginosa biofilms on chronic non‐
healing diabetic wounds in a murine model Jacob Gibson Michigan State University, East Lansing, MI, USA Bacterial stimulation of toll‐like receptors drives macrophages to hemophagocytose Erin McDonald University of Colorado, Boulder, CO, USA 59th Annual Wind River Conference, June 10 ‐ 14, 2015 9
11:50‐12:10 PM 12:15‐12:30 PM 12:30‐2:00 PM 2:00‐4:30 PM 4:30‐6:30 PM 6:30‐7:30 PM 8:00‐9:00 PM 9:00 ‐ 7:00‐8:30 Metal homeostasis in E. coli: the predicted manganese chaperone, MntS, and the manganese exporter, MntP Lauren Waters University of Oshkosh, Oshkosh, WI, USA The role of Mfd in oxidative damage repair Kate Porter UNLV, Las Vegas, NV, USA Lunch (Great Room Dining Room) Free Time Poster Session and Happy Hour Dinner (Great Room Dining Room) Identification of a peptide‐pheromone that enhances Listeria monocytogenes escape from the confines of mammalian cell vacuoles
Nancy Freitag University of Illinois‐Chicago Happy Hour at the lounge and bar Sunday, June 14, 2015 Breakfast (Great Room Dining Room) Departure 59th Annual Wind River Conference, June 10 ‐ 14, 2015 10
List of Poster Presentations Poster Placement:
1 - 15 = Meeker, 16 - 31 = Lady Washington
1. Junqin(Heidi) Bai, Univ. of Illinois‐Chicago. Title: Identification of an Interaction between the Competence Regulator ComX and ComW of S. pneumoniae by a Yeast Two‐Hybrid Assay 2. Biswanath Jana, Univ. of Kansas Medical Center, Title: Substrate identification by ClpXP protease complex in Streptococcus mutans 3. Pratick Khara, Univ. of Kansas Medical Center, Title: The uniqueness of global response regulator CovR of Streptococcus mutans 4. Jacob E. Choby, Vanderbilt University, Title: A small molecule inhibitor is toxic to Staphylococcus aureus in an Sae‐dependent manner 5. William H. Broach, Univ. of South Florida, Title: A comparative genomics exploration of three diverse staphylococcal transcriptomes gives insight into sRNA evolution 6. James A. Budnick, Virginia Tech, Title: No GABA GABA! A putative ABC transporter of GABA is essential for the ability of Brucella abortus to infect and colonize mice 7. Logan L. Bulock, Univ. of Nebraska Medical Center, Title: Comparative analysis of metabolic gene expression during Staphylococcus aureus biofilm development. 8. David J. Samuels, Georgetown University, Title: Reducing CodY activity using GTP limitation alters metabolism and virulence gene expression in Staphylococcus aureus 9. John R. Harp, Univ. of Tennessee, Title: Membrane alterations contribute to daptomycin resistance in Enterococcus faecalis 10. Jared Hoffman, Brigham Young, Title: RfaH regulates lipopolysaccharide synthesis in Yersinia pestis and Yersinia pseudotuberculosis and is required for resistance to antimicrobial chemokines 11. Kimberly McCullor, Univ. of Oklahoma Health Sciences Center, Title: Streptococcus anginosus daptomycin‐resistant clinical strain J4206: Clues to its virulence 12. Megan E. Fris, Ohio University, Title: RyfA and RyfB in Shigella 13. Ryan M. Mettetal, Sharklet Technologies, Title: Surface micropattern resists bacterial contamination transferred by healthcare practitioners 14. Toni A. Nagy, Univ. of Colorado, Title: A tale of two niches: Sites of chronic Salmonella infection 15. Nicole Inniss, Univ. of Illinois‐Chicago, Title: A Comparison of Native and Non‐Native Purification of the Pneumococcal Competence Specific Regulatory Protein, ComW 59th Annual Wind River Conference, June 10 ‐ 14, 2015 11
16. R. Martin Roop II, East Carolina University, Title: The Mn2+‐dependent pyruvate kinase PykM is a critical virulence determinant for Brucella abortus 2308 in the mouse model 17. Rodolfo Urbano, Univ. of Washington, Title: Antimicrobial nitric oxide targets AgrA of the Staphylococcus aureus Agr system to inhibit quorum sensing and suppress virulence 18. Tatiana S. Ermi, UNLV, Title: Using the soil microbe Bacillus subtilis to elucidate the role of gene expression in response to environmental stresses. 19. Haibi Wang, Univ. of Wyoming, Title: A novel regulator of chromosome segregation 20. Rebecca A. Keogh, Virginia Tech, Title: Characterization of an ArsR‐family transcriptional regulator and its importance to the virulence of Brucella abortus 21. Maliha Rahman, Univ. of Oklahoma Health Sciences Center, Title: Transcriptome analysis of Streptococcus pyogenes Chromosomal Island (SpyCI) 22. Michael A. Olson, Brigham Young, Title: Establishing a Galleria mellonella model to study Escherichia coli mediated mastitis 23. Andy Weiss, Univ. of South Florida, Title: Characterization of the structural and functional importance of the RNA polymerase omega subunit in Staphylococcus aureus 24. Natasha Weatherspoon‐Griffin, UNLV, Title: Characterization of SlyA in Shigella flexneri 2457T reveals a role in acid resistance 25. Devon N. Marking, Univ. of South Florida, Title: Exploring the role of intracellular aminopeptidases in Staphylococcus aureus pathogenesis 26. Tramy N. Hoang, Univ. of Nebraska Medical Center, Title: Function of IcaR and TcaR in icaADBC regulation in Staphylococcus epidermidis 27. Abigail L. Reens, Univ. of Colorado, Title: Host fatty acids as a nutrient source for Salmonella during infection of macrophages 28. Corey P. Parlet, Univ. of Iowa, Title: Apicidin inhibits Staphylococcus aureus quorum sensing‐
mediated pathogenesis 29. Catarina G. Fernandes, Universidade Nova de Lisboa and Emory University, Title: The assembly of a sporulation enzyme is driven by its substrates 30. Loni Townsley, Univ. of California‐Santa Cruz, Title: Cold shock gene cspV regulates type 6 secretion and biofilm formation in Vibrio cholera 31. Don Ennis, Univ. of Lousiana, Title: Analyses of gene expression profiles in Mycobacterium marinum in an activated virulent state 59th Annual Wind River Conference, June 10 ‐ 14, 2015 12