Shoji Lab. Waseda Univ. Page 8 Fabrication

STAND-ALONE MICRO FLUIDIC SYSTEM
USING PARTLY DISPOSABLE PDMS
MICROWELL ARRAY FOR HIGH
THROUGHPUT CELL ANALYSIS
Kasumi Miyamoto1, Ryo Yamamoto1,
Kentaro Kawai2, and Shuichi Shoji1
1Major
in Nano-Science and Nano-Engineering, Waseda University, Tokyo, JAPAN
2Department of Precision Science and Technology, Osaka University, JAPAN
Contents
1. Background
 Microfluidic Devices and Microvalves
 Problems and Resolution
2. Stand-alone microfluidic system
 System Concept, Principle,
Fabrication, Device Design, System Setup
 Microfluidic Experiment
 System Performance
3. Conclusion
4. Future Work
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Shoji Lab. Waseda Univ.
Background of Microvalves
Microfluidic devices
Application to microwell array chip
Microwells
Microvalves
Y. Shibata et al., μTAS 2009, vol.2, pp1488-1491
Integration of microvalves into microfluidic devices
Pneumatic valve
 simple structure
 not expensive to fabricate
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Shoji Lab. Waseda Univ.
Problems with Pneumatic Valves
However...employing pneumatic valves requires
Bulky instruments
Troublesome
world-to-chip connection
Complicated, laborious
fabrication
Air compressor
Vlad Savov, et.al, 2009
Objective is to fabricate microfluidic system to be
Stand-alone ＆ Access-tube-free ＆ Partly reusable
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Shoji Lab. Waseda Univ.
Resolution to the Problems
Stand-alone ＆ Access-tube-free ＆ Partly reusable
valve control layer “reusable”
solenoid
actuator
EOF micropump
fluidic chip “disposable”
Bulky instruments
 air compressor
 syringe pump
World-to-chip connectors
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solenoid actuator
EOF micropump
exchangeable
Shoji Lab. Waseda Univ.
Proposed Microfluidic System
Items to be fulfilled
 access-tube-free
 stand-alone
 partly reusable
+α
 several types of
fluidic chip applicable
 easy to use
 all PDMS
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Shoji Lab. Waseda Univ.
Principle of Fluidic Control
(a) Valve closed
(b) Valve open
1. Voltage applied
1. Voltage turned off
2. Plunger propelled down
2. Plunger propelled up
3. Membrane inflated
3. Membrane relaxed
4. Reagent flow interrupted
4. Reagent flow continued
Valves actuated in 19msec (open→closed)
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Shoji Lab. Waseda Univ.
Fabrication
Reusable valve control part
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Disposable fluidic chip part
Shoji Lab. Waseda Univ.
Fabricated System
Compact system size by 140mm×150mm×65mm
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Shoji Lab. Waseda Univ.
Fluidic Chip Design
16 microwell array and 4 reagent inlet lines are designed
dimension: 30mm×40mm
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Shoji Lab. Waseda Univ.
System Setup
1. Set valve control layer and fluidic chip
Valve control layer
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Fluidic chip
Shoji Lab. Waseda Univ.
System Setup
2. Connect solenoid actuators and EOF micropumps
to power source regulated by computer
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Shoji Lab. Waseda Univ.
Multi-reagent Exchange
Used Reagents
Red: rhodamine B
Blue: methylene blue
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Shoji Lab. Waseda Univ.
Multi-reagent Exchange
Reagent exchange is successfully carried out by
selectively introducing blue/red ink
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Shoji Lab. Waseda Univ.
System Performance
Power Consumption
Solenoid actuators
11.86Wh
EOF micropump
0.024Wh
Total
11.88Wh
System Summary
property
value
On-chip valves
Solenoid actuators
Chip alternation
Power consumption
20
10
exchangeable
11.88Wh
Able to run in high efficiency even in low power supply
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Shoji Lab. Waseda Univ.
Conclusion
We established stand-alone, access-tube-free,
fluidic-chip-exchangeable microfluidic system
We achieved 19msec for switching on-chip valves
We succeeded to exchange multiple reagents
in the microfluidic experiment
The proposed system can run in high efficiency
even in low power consumption
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Shoji Lab. Waseda Univ.
Future Work
Change material to improve valving performance
All PDMS
PDMS+PMMA
PMMA (polymethyl methacrylate)
Overcome problems such as…
 pressure loss
 low airtightness
 shrinkage of PDMS
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Shoji Lab. Waseda Univ.
Acknowledgements
Fabrication process has been partly carried out at Waseda
University Nanotechnology Research Center (NTRC).
This work is partly supported by the Japan Ministry of
Education, Culture, Sports Science and Technology
Grant-in-Aid for Science Basic Research (A) No.
19206046 of Waseda University, Grovel COE program
“Ambient GCOE” of Waseda University.
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Shoji Lab. Waseda Univ.
Thank you for your attention!!
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Shoji Lab. Waseda Univ.