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Preface CMU Research Abstracts 2010 is a representative collection of 481 research abstracts produced by CMU-affiliated researchers focusing on innovative research and development projects stimulated by national, regional, and international development policies. Research and development is essential to any university seeking to establish and maintain recognition in the international education network, as well as to further its goals of serving the greater public good through education, scholarship, and innovation. Pursuant to CMU’s vision, “Chiang Mai University is a Leading University with Academic Excellence in International Standards, focusing to become a research-oriented institution of higher education and producing graduates with high moral and ethical standards, equipped to practice good governance under the Sufficiency Economy Philosophy and Sustainable Development,” Chiang Mai University continues to focus on initiating, improving, and implementing programs designed to raise its standards of both academic excellence and innovative research, helping CMU toward its goal of becoming the leading university-level research facility in Thailand. In addition, through research like that represented here, CMU is generating increased interest by national and international organizations for collaboration on joint research projects. Since 1987, CMU researchers have contributed 5,833 research abstracts to CMU’s Resource Archives (http://cmupress.cmu.ac.th). The abstracts here in the CMU Research Abstracts 2010 are representative of the standard of excellence of our researchers, and a fine illustration of their commitment to sharing the benefits of their knowledge and skills to improve the quality of life locally, regionally, and globally. I would like to express my sincere gratitude and appreciation to the lecturers and researchers for their scholarship, and to the members of the CMU Abstract Committee, without whose invaluable efforts, the compilation of this publication would not have been possible. Pongsak Angkasith, Ed.D. President Contents I. The abstacts of full papers publication research institute for health sciences 1. GROWTH AND DEVELOPMENT OF PRESCHOOL CHILDREN IN CHILD DAY CARE CENTERS IN CHIANG MAI 3 2. IODINE STATUS OF PRESCHOOL CHILDREN IN CHILD DAY CARE CENTERS IN CHIANG MAI 4 Faculty of Associated medical sciences 1. A PROPHYLACTIC EFFECT OF MASSAGE ON SYMPTOMS OF MUSCLE DAMAGE INDUCED BY ECCENTRIC EXERCISE OF THE WRIST EXTENSORS 5 2. A PROPHYLACTIC EFFECT OF PROPRIOCEPTIVE NEUROMUSCULAR FACILITATION (PNF) STRETCHING ON SYMPTOMS OF MUSCLE DAMAGE INDUCED BY ECCENTRIC EXERCISE OF THE WRIST EXTENSORS 6 3. EFFECTS OF UP-AND DOWNSLOPE WALKING ON MEAN AND VARIABILITY OF GAIT PARAMETERS IN ELDERLY WOMEN 7 4. IMPACT OF FOOTWEAR STYLES ON POSTURAL CONTROL ABILITY OF FEMALE ELDERLY 8 5. RELIABILITY OF MUSCLE FUNCTION AND SENSORY PERCEPTION MEASUREMENTS OF THE WRIST EXTENSORS 9 6. SENSORY PROFILE ASSESSMENT TOOL FOR CHILDREN 0-36 MONTHS OF AGE, THAI VERSION 10 7. USING WEIGHTED VEST TO INCREASE ATTENTION IN CHILDREN WITH HYPERACTIVITY 11 Faculty of Medicine 1. A 3-YEAR FOLLOW-UP OF ANTIBODY RESPONSE IN HIV-INFECTED CHILDREN WITH IMMUNE RECOVERY VACCINATED WITH INACTIVATED JAPANESE ENCEPHALITIS VACCINE 12 2. 13 A CHEWABLE PEDIATRIC FIXED-DOSE COMBINATION TABLET OF STAVUDINE, LAMIVUDINE, AND NEVIRAPINE PHARMACOKINETICS AND SAFETY COMPARED WITH THE INDIVIDUAL LIQUID FORMULATIONS IN HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CHILDREN IN THAILAND 3. A RETROSPECTIVE STUDY OF OUTCOME IN PEDIATRIC LUPUS NEPHRITIS AFTER THE MAINTENANCE THERAPY BY AZATHIOPRINE OR ECMPS 15 4. AIR POLLUTION-RELATED PEAK EXPIRATORY FLOW RATES AMONG ASTHMATIC CHILDREN IN CHIANG MAI, THAILAND 16 5. 17 AMBIENT PARTICULATE POLLUTION AND THE WORLD-WIDE PREVALENCE OF ASTHMA, RHINOCONJUNCTIVITIS AND ECZEMA IN CHILDREN: PHASE ONE OF THE INTERNATIONAL STUDY OF ASTHMA AND ALLERGIES IN CHILDHOOD (ISAAC) 6. AN ENZYME-LINKED IMMUNOSORBENT ASSAY AS SCREENING TOOL FOR HUMAN INTESTINAL CAPILLARIASIS 19 7. ANALYSIS OF FEMALE SALIVARY GLAND PROTEINS OF THE ANOPHELES BARBIROSTRIS COMPLEX (DIPTERA: CULICIDAE) IN THAILAND 20 8. Anesthesia-related complications of caesarean delivery in Thailand: 16,697 cases from the Thai Anaesthesia Incidents Study 21 i 9. ANTIRETROVIRAL THERAPY OUTCOMES OF HIV-INFECTED CHILDREN IN THE TREAT ASIA PEDIATRIC HIV OBSERVATIONAL DATABASE 23 10. Bone resorption acceleration and calcium reabsorption impairment in a Thai population with high cadmium exposure 24 11. Cadmium induced renal dysfunction among residents of rice farming area downstream from a zinc-mineralized belt in Thailand 25 12. 26 CELL RESERVOIRS OF THE EPSTEIN-BARR VIRUS IN BIOPSY-PROVEN LYMPHOCYTIC INTERSTITIAL PNEUMONITIS IN HIV-1 SUBTYPE E INFECTED CHILDREN IDENTIFICATION BY COMBINED IN SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY 13. CHARACTERISTICS OF LYMPHOCYTE SUBSETS IN HIV-INFECTED, LONG-TERM NONPROGRESSOR, AND HEALTHY ASIAN CHILDREN THROUGH 12 YEARS OF AGE 27 14. 29 CHEMICAL COMPOSITION AND LARVICIDAL ACTIVITY OF EDIBLE PLANT-DERIVED ESSENTIAL OILS AGAINST THE PYRETHROID- SUSCEPTIBLE AND -RESISTANT STRAINS OF AEDES AEGYPTI (DIPTERA: CULICIDAE) 15. Comparison of efficacy and effectiveness between ULTRACET and tramadol/acetaminophen in acute postoperative pain after upper extremity surgery 30 16. CORD BLOOD SCREENING FOR ALPHA-THALASSEMIA AND HEMOGLOBIN VARIANTS BY ISOELECTRIC FOCUSING IN NORTHERN THAI NEONATES: CORRELATION WITH GENOTYPES AND HEMATOLOGIC PARAMETERS 31 17. DEFERASIROX IN IRON-OVERLOADED PATIENTS WITH TRANSFUSION-DEPENDENT MYELODYSPLASTIC SYNDROMES: RESULTS FROM THE LARGE 1-YEAR EPIC STUDY 32 18. Diagnosing delirium in elderly Thai patients: utilization of the CAM algorithm 33 19. DIFFERENTIATION BETWEEN LUCILIA CUPRINA AND HEMIPYRELLIA LIGURRIENS (DIPTERA: CALLIPHORIDAE) LARVAE FOR USE IN FORENSIC ENTOMOLOGY APPLICATIONS 34 20. EFFECT OF LANNA MUSIC LISTENING AND MASSAGE ON EMOTIONAL AND PHYSIOLOGICAL CHANGE: PRELIMNARY DATA FROM THAI VOLUNTEER GROUP 35 21. FORENSICALLY IMPORTANT FLESH FLY SPECIES IN THAILAND: MORPHOLOGY AND DEVELOPMENTAL RATE 36 22. Fractures of the hyoid bone and thyroid cartilage in suicidal hanging 37 38 23. FULMINANT ENTEROVIRUS 71 INFECTION: CASE REPORT 24. HIV-1 DRUG RESISTANCE MUTATIONS IN CHILDREN AFTER FAILURE OF FIRST-LINE NONNUCLEOSIDE REVERSE TRANSCRIPTASE INHIBITOR-BASED ANTIRETROVIRAL THERAPY HIV-1 DRUG RESISTANCE MUTATIONS IN CHILDREN AFTER FAILURE OF FIRST-LINE NONNUCLEOSIDE REVERSE TRANSCRIPTASE INHIBITOR-BASED ANTIRETROVIRAL THERAPY 39 25. INTER-SPECIFIC GENE FLOW DYNAMICS DURING THE PLEISTOCENE-DATED SPECIATION OF FOREST-DEPENDENT MOSQUITOES IN SOUTHEAST ASIA 41 26. LATE EFFECTS IN SURVIVORS OF CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA: A STUDY FROM THAI PEDIATRIC ONCOLOGY GROUP 43 27. MEASLES OUTBREAK IN AN ORPHANAGE: HIV-INFECTED CHILDREN ON ANTIRETROVIRAL THERAPY ARE STILL AT RISK 44 ii 28. MEDIASTINAL PANCREATIC PSEUDOCYST IN CHRONIC PANCREATITIS 45 29. MULTIPLEX ASSAY TO IDENTIFY KOREAN VECTORS OF MALARIA 46 30. PERSISTENCE OF MEASLES, MUMPS, AND RUBELLA PROTECTIVE ANTIBODIES 3 YEARS AFTER REVACCINATION IN HIV-INFECTED CHILDREN RECEIVING ANTIRETROVIRAL THERAPY 47 31. PERSISTENT HYPERINSULINEMIC HYPOGLYCEMIA OF INFANCY ASSOCIATED WITH CONGENITAL NEUROBLASTOMA: A CASE REPORT 48 32. POOR COGNITIVE FUNCTIONING OF SCHOOL-AGED CHILDREN IN THAILAND WITH PERINATALLY ACQUIRED HIV INFECTION TAKING ANTIRETROVIRAL THERAPY 49 33. Predictors and consequences of incarceration among a sample of young Thai methamphetamine users 50 34. PRODUCTION OF MONOCLONAL ANTIBODIES TO P-GLYCOPROTEIN: ITS APPLICATION IN DETECTION OF SOLUBLE AND SURFACE P- LYCOPROTEIN OF LEUKEMIA PATIENTS 51 35. RAPID DETECTION OF DIROFILARIA IMMITIS IN MOSQUITO VECTORS AND DOGS USING A REAL-TIME FLUORESCENCE RESONANCE ENERGY TRANSFER PCR AND MELTING CURVE ANALYSIS 52 36. RELATIONSHIP OF CELL BEARING EBER AND p24 ANTIGENS IN BIOPSY-PROVEN LYMPHOCYTIC INTERSTITIAL PNEUMONIA IN HIV-1 SUBTYPE E INFECTED CHILDREN 53 37. REPORT OF THE 11TH WORLD CONGRESS OF PEDIATRIC DERMATOLOGY IN BANGKOK, THAILAND 55 38. SIZE AND CHARGE ANTIGENS OF DIROFILARIA IMMITIS ADULT WORM FOR IGG-ELISA DIAGNOSIS OF BANCROFTIAN FILARIASIS 56 39. SPATIAL GENETIC STRUCTURE OF AEDES AEGYPTI MOSQUITOES IN MAINLAND SOUTHEAST ASIA 57 40. SYSTEMATICS OF ANOPHELES (CELLIA) YAEYAMAENSIS SP. N., ALIAS SPECIES E OF THE AN. MINIMUS COMPLEX IN SOUTHEASTERN ASIA (DIPTERA: CULICIDAE) 58 41. The Thai Anesthesia Incident Monitoring Study (Thai AIMS): an analysis of perioperative complication in geriatric patients 59 42. Women’s Health survey of female prisoners at Chiang Mai Women Prison 61 Faculty of pharmacy 1. Determination of Clozapine in Human Plasma by High–Performance Liquid Chromatography with UV–VIS Detector 62 2. Inhibitory effects of caffeic acid ester analogues on free radicals and human liver microsome CYP1A2 activities 63 Institute for science and technology research 1. A MICROFLOW CHEMILUMINESCENCE SYSTEM FOR DETERMINATION OF CHLORAMPHENICOL IN HONEY WITH PRECONCENTRATION USING A MOLECULARLY IMPRINTED POLYMER 64 2. BIOLOGICAL ACTIVITIES OF PHENOLIC COMPOUNDS ISOLATED FROM GALLS OF TERMINALIA CHEBULA RETZ. (COMBRETACEAE) 65 3. BIOLOGICAL ACTIVITIES OF THE RICE BRAN EXTRACT AND PHYSICAL CHARACTERISTICS OF ITS ENTRAPMENT IN NIOSOMES BY SUPERCRITICAL CARBON DIOXIDE FLUID 66 iii 4. EFFECTS OF VIBRO-MILLING ON RELAXOR FERROELECTRIC BEHAVIOR AND PHASE TRANSITION OF LEAD-FREE Ba(Zr0.25Ti0.75)O3 CERAMICS 67 5. ENHANCEMENTOF TRANSDERMAL ABSORPTION GENE EXPRESSION AND STABILITY OF TYROSINASE PLASMID (pmel34)-LOADED ELASTIC CATIONIC NIOSOMES: POTENTIAL APPLICATION IN VITILIGO TREATMENT 68 6. ENTRAPMENT ENHANCEMENT OF PEPTIDE DRUGS IN NIOSOMES 69 7. FREE RADICAL ACAVENGING AND TYROSINASE INHIBITION ACTIVITY OF OILS AND SERICIN EXTRACTED FROM THAI NATIVE SILKWORMS (BOMBYX MORI) 70 8. HARMONIC ANALYSIS OF DYNAMIC HYSTERESIS RESPONSE OF BaTiO3 BULK CERAMICS 71 9. HIGH-ENERGY HEAVY ION BEAM ANNEALED ION-IMPLANTATION SYNTHESIZED SiC NANOCRYSTALLITES AND PHOTOLUMINESCENCEH 72 10. IN VITRO ANTI-AGING ACTIVITIES OF TERMINALIA CHEBULA GALL EXTRACT 73 74 11. LEAD-FREE HIGH REFRACTIVE INDEX GLASS USING LOCAL THAI SANDS 12. MODELING OF FERROELECTRIC HYSTERESIS AREA OF HARD LEAD ZIRCONATE TITANATE CERAMICS: ARTIFICIAL NEURAL NETWORK APPROACH 75 13. NOVEL FERROCENIC STEROIDAL DRUG DERIVATIVES AND THEIR BIOACTIVITIES 76 77 14. PHASE TRANSITION BEHAVIOR IN (1Zx)PZT—xBiAlO3 CERAMICS 15. RELATIONSHIP BETWEEN SUSCEPTIBILITY TO ANTHRACNOSE DISEASE AND ANTIFUNGAL COMPOUNDS CONTENT IN FRUIT LATEX OF THAI MANGO 78 16. SEQUENTIAL INJECTION ANALYSIS WITH LAB-AT-VALVE (SI-LAV) FOR THE DETERMINATION OF SOLASODINE IN SOLANUM SPECIES 79 17. STORAGE LIFE EXTENSION OF EXPORTED ‘NAM DOKMAI’ MANGO BY REFRIGERATED MODIFIED ATMOSPHERE PACKING 80 18. THE DEBYE DIELECTRIC BEHAVIOR OF MIXED NORMAL AND RELAXOR-FERROELECTRICS: MONTE CARLO INVESTIGATION 81 19. TRANSDERMAL ABSORPTION ENHANCEMENT THROUGH RAT SKIN OF GALLIDERMIN LOADED IN NIOSOMES 82 20. WEBCAM CAMERA AS A DETECTOR FOR S SIMPLE LAB-ON-CHIP TIME BASED APPROACH 83 Faculty of Agriculture 1. Effects of Some Cytokinins, Auxins and Medium Constituents on In Vitro Propagation of Polystachya sp. Faculty of Agro-Industry 84 1. ANTIMICROBIAL ACTIVITY OF OLIGOMER AND POLYMER CHITOSAN FROM DIFFERENT SOURCES AGAINST FOOD BORNE PATHOGENIC BACTERIA 85 2. APPROACH FOR LOGOS DESIGN USING MATRIX ANALYSIS TABLE CASE STUDY: LOGOS DESIGN FOR HOTEL IN CHIANG MAI PROVINCE 86 3. CHARACTERIZATION OF CARBOXYMETHYL CELLULOSE FILM FROM Mimosa Pigra 87 4. DEVELOPMENT OF CHITOSAN FILM INCORPORATING GARLIC OIL AND POTASSIUM SORBATE AS ANTIMICRIBIAL AGENTS FOR GARLIC BREAD 88 iv 5. DIFFERENCES OF SOME NUTRIRTIONAL AND PHYSICAL PROPERTIES of NORTHERN THAI-STYLE FERMENTED SOYBEANS (THUA-NAO) DRIED BY THREE DIFFERENCT METHODS 89 6. EFFECT OF AMOUNT OF BLENDED STARCH ADHESIVES AND PARTICLE SIZES FROM TAMARIND BARK ON PROPERTIES OF PARTICLEBOARD 90 7. EFFECT OF ANTIOXIDANT CONCENTRATION ON WATER VAPOR TRANSMISSION RATE AND SORPTION ISOTHERM OF CASSAVA STARCH-CARBOXYMETHYL CELLULOSE (CMC) FILMS 91 8. EFFECT OF NaOH CONCENTRATION ON SORPTION ISOTHERM OF CARBOXYMETHYL RICE STARCH FILMS AND PREDITION MODELS 92 9. EFFECT OF PLASMA TREATMENT ON HYDROPHOBICITY AND BARRIER PROPERTIES OF POLYLACTIC ACID 93 10. EFFECT OF SULPHUR HEXAFLUORIDE (SF6) PLASMA ON HYDROXPHOBICITY OF METHYL CELLULOSE FILM 94 11. EFFECT OF UREA FORMALDEHYDE ON PHYSICAL AND MECHANICAL PROPERTIES OF PARTICLEBOARD FROM COFFEE WASTE 95 12. FLUORINATED DIAMOND-LIKE CARBON COATING ON POLYLACTIC ACID FILM PREPARED BY PLASMA-ENHANCED CHEMICAL VAPOR DEPOSITION 96 13. INNOVATION MANAGEMENT THE NEW APPROACH FOR SMALL MEDIUM ENTERPRISE (SME) 97 14. MORPHOLOGY, PHYSICAL AND CHEMICAL PROPERTIES OF QUEEN PINEAPPLE FRUIT 98 15. PREDICTION MODELS FOR MOISTURE SORPTION ISOTHERMS OF SOY PROTIEN ISOLATE/CARBOXYMETHYL CHITOSAN BLEND FILMS 99 16. PREDICTION MODELS FOR SHELF LIFE OF PUMPKIN CRACKERS IN DIFFERENT PACKAGES BASED ON ITS MOISTURE CONTENT 100 18. STUDY OF CARBOXYMETHYL CELLULOSE FROM PAPAYA PEELS AS BINDER IN CERAMICS 101 19. SURFACE MODIFICATION AND PERMEABILITY ANALYTICAL STUDIES OF POLYLACTIC ACID INCUCED BY HMDSO-PLASMA 102 Faculty of Engineering 1. A Box-splitting Strategy of Interval Newton Method for Finding All DC Solutions of BJT Circuits 103 2. A Combustion Aerosol Generator for Submicron Aerosol Production 104 3. A particle swarm optimization for wind energy control problem 105 4. A STUDY ON THE FEASIBILITY OF ZERO-EMISSIONS AIR TRANSPORT 106 5. A Wide-Band Bandpass Filter Using a Novel Embedded Short-Circuited Stub Resonator 107 6. ACOUSTIC-BASED SIGNAL TRANSMISSION FOR MANGOSTEEN INTERNAL QUALITY MEASURES 108 7. An Interior Point Method for WLAV State Estimation of Power System with UPFCs 109 8. ANALYSIS OF BATTERY USING MICROCONTROLLER 110 9. ANALYSIS OF DIESEL ENGINE PERFORMANCE WITH MAYAOHIN AS A FUEL 111 10. Analytical discussions on species extinction in competitive communities due to habitat destruction 112 v 11. Application of Indirect X-Ray Detector by using Contact Image Sensor couple with Field Programmable Gate Array and Mini-CT 113 12. Application of Remote Devices Vital-Signs to be Included Telemedicine Mobile Table on WiMAX Network 114 115 13. APPLICATIONS OF LIFE CYCLE ASSESSMENT TO SUSTAINABLE IDEA 14. Appraisal of Appropriate Combustion Air in a Pulverized Coal-fired Boiler 116 15. ARSENIC, CHROMIUM, AND COPPER LEACHING FROM CCA-TREATED WOOD AND THEIR POTENTIAL IMPACTS ON LANDFILL LEACHATE IN A TROPICAL COUNTRY 117 16. Assessment of Lubricating Oil Degradation in Small Motorcycle Engine Fueled with Gasohol 118 17. BIO-ETHANOL PRODUCTION FROM POTATO WASTE 119 18. Biogas Production from Animal Waste Using Hybrid Reactor 120 19. Biogas Quality Upgrade by Simultaneous Removal of CO2 and H2S in a Packed Column Reactor 121 20. Chemical and physical properties of rice straw waste and hospital sewage sludge in turned windrow aeration system 122 21. COMPARISON OF COOLING SYSTEMS WITH CELLULOSE VERSUS GUNNYSACK USING EXCESS COOL WATER FROM ICE FACTORY 123 22. Continuous Biodiesel Production from Transesterification of Jatropha Oil with Microwave Heating 124 125 23. Control Strategy for Biogas’s Continuous Stirred Tank Reacto 24. Conversion of Bamboo into Glucose by Dilute Acid and Enzymatic Hydrolysis 126 25. Co-operational Optimization of Two Cascade Hydropower Plants 127 26. Design and Theoretical Investigation of a PM2.5 Impactor for Airborne Particulate Matter Sampling 128 27. DESIGN OF LOW SPEED PERMANENT MAGNET GENERATOR 129 28. Development and Validation of a Battery Model Useful for Discharging and Charging Power Control and Lifetime Estimation 130 29. Development of Drop-on-Demand Printer for Rapid Prototyping Applications 131 132 30. DEVELOPMENT OF DRYING KINETICS EQUATIONS OF BANANA SLICE 31. DEVELOPMENT OF MANGO WOOD-CARVING DRYING SYSTEM USING HEAT EXCHANGER FROM BIOMASS FUEL 133 32. Development of Monitoring and Control System Using Distributed Embedded System Via Can Bus 134 33. Dilute Acid Pretreatment of Bamboo for Fermentable Sugar Production 135 34. Domestic wastewater treatment by a constructed wetland system planted with rice 136 35. Drop-on-Demand Printer for Micro Ion-Selective Electrode Fabrication 137 138 36. DRYING OF BANANA USING SUPERHEATED STEAM FOLLOWED BY HOT AIR vi 37. Durability of a Small Agricultural Engine on Biogas/Diesel Dual Fuel Operation 139 38. EFFECT OF BOUND NUMBER ON HEAT TRANSFER OF THERMOSYPHON HEAT PIPE UNDER SOUND WAVE 140 39. Effect of Needle Cone Angle and Air Flow Rate on Electrostatic Discharge Characteristics of a Corona-needle Ionizer 141 40. Effects of Aspect Ratios and Number of Meandering Turns on Performance Limit of an Inclined Closed-Loop Oscillating Heat Pipe 142 41. EFFECTS OF LIP THICKNESS ON ALVEOLAR BONE GRAFT OUTCOMES IN PATIENTS WITH COMPLETE UNILATERAL CLEFT LIP AND PALATE : FINITE ELEMENT ANALYSIS 143 42. Effects of Reaction Forces at Clamped Edge on Deflection and Stress of Thin Plate 144 43. Effects of Seeding on Performance of Household Organic Waste Composting Using Passive Aeration 145 44. EFFECTS OF TEMPERATURE, VELOCITY AND RELATIVE HUMIDITY OF HOT AIR ON SLICED BANANA DRYING 146 147 45. ELECTRIC CYLINDER ENGINE 46. ENHANCMENT OF ELECTROSTATIC PRECIPITATOR FOR SUBMICRON PARTICLE COLLECTION USING NON-THERMAL PLASMA TECHNIQUE 148 47. EVALUATION OF ELECTRICITY GENERATION POTENTIAL FROMWIND TURBINE FARM AT MHAE ROYAL PROJECT SAMOENG DISTRICT, CHIANG MAI PROVINCE,THAILAND 149 48. Evaluation of Energy Absorption Capability of Light Truck’s Front Nudge Bar 150 49. Experimental Investigation of an Automotive Air Conditioning System Driven by a Small Biogas Engine 151 50. Finding All DC Solutions of Diode and BJT Circuits by Interval Method and Modified LP-Narrowing Technique 152 153 51. Gasification of Giant Sensitive Plants in a Fixed Bed Reactor 52. Heat and mass transfer in combined convective and far-infrared drying of fruit leather 154 53. Heat extraction from salinity-gradient solar ponds using heat pipe heat exchangers 155 54. Identification of hyperelastic material parameters using Virtual Fields Method in case of large deformations 156 55. Image Restoration Based on a Pair of Noisy and Motion Blurred Images 157 56. In vivo characterization of the aortic wall stress-strain relationship 158 57. Incorporating SIFT with Hard C-Means Algorithm 160 58. Investigation of Light Tar Cracking in a Gliding Arc Plasma System 161 59. Kinetics and modeling of whole longan with combined infrared and hot air 162 163 60. LEACHING OF HEAVY METAL FROM SPENT HOUSEHOLD BATTERIES 61. Life Cycle Assessment of Frozen Okra Production by Parameter Screening Method 164 vii 62. Mathematical Model of the Optimum Heat Pipe Heat Exchanger for a Condenser of Vapor-Compression Refrigeration Cycle 165 166 63. Medical Consult Base System for Diagnosis on WiMAX System 64. Microcalcification Detection in Mammograms Using Interval type-2 Fuzzy logic System with Automatic Membership Function Generation 167 65. Modeling of a rigid-flexible manipulator using Hamilton’s principle 168 66. Modified Kinetic Model of Particle Detachment by Aerodynamic Drag and Vibration 169 67. Moisture Content Prediction of Dried Longan Aril from Dielectric Constant Using Multilayer Perceptrons and Support Vector Regression 170 68. Non-isothermal pyrolysis characteristics of giant sensitive plants using thermogravimetric analysis 171 69. Numerical Computation of Fluid Flow and Aerosol Transport in a Long Electrical Mobility Spectrometer 172 70. Optimal Choice and Allocation of Distributed Generations Using Evolutionary Programming 173 71. Particulate Emission Reduction from Biomass Burning in Small Combustion Systems with a Multiple Tubular Electrostatic Precipitator 174 72. PERFORMANCE AND EMISSION OF AGRICULTURAL DIESEL ENGINE USING PALM-DIESEL EMULSION AS FUEL 175 73. Performance Evaluation of NTPv4 When SPIK State Cannot Step Frequency 176 74. Performance of Wire-on-tube Heat Exchanger Using OscillatingHeat Pipe Fin, A Case Study of Free Convection 177 178 75. Piezo Actuated Print Head System for Drop-On-Demand Printer 76. POTENTIAL OF DENDROTHERMAL POWER GENERATION IN THAILAND AND ITS CONCERNS 179 180 77. Processing of hornblende syenite for ceramics 78. Production of Charcoal from Woods and Bamboo in a Small Natural Draft Carbonizer 181 79. Production Cost Analysis in an Aluminum Die-Casting Department Using Activity-Based Costing System 182 80. Quantification of Liquid Water Saturation in a Transparent Single-Serpentine Cathode Flow Channel of PEM Fuel Cell by Using Image Processing 183 81. RECOVERING AND UPGRADING WASTE HEAT OF AIR-CONDITIONER BY COMBINING R-123 VAPOR COMPRESSION HEAT PUMP 184 82. Selection of efficient wavelengths in NIR spectrum for determination of dry matter in kiwi fruit 185 186 83. Simulation of Cassava Chip Drying using Convective Hot-Air 84. Temperature Reduction in Hydroponic Planting System Using Heat Pipe 187 85. THE ANALYSIS OF ENERGY CONSUMPTION AND CARBON DIOXIDE EMISSIONS IN THAI MANUFACTURING SECTOR BY DECOMPOSITION TECHNIQUE 188 viii 86. The design and performance of constructed wetlands for wastewater treatment in tropical regions 189 190 87. THE STUDY OF BIOCHAR PROPORTY FROM SLOW PYROLYSIS OF ALGAE 88. The Study of Maximum Load of Thin-wall Paper Tube Under Axial Compressive Load 191 89. THE TEST OF A MODULAR CHEMICAL ENERGY STORAGE SYSTEM USING SODIUMSULPHIDE-WATER AS WORKING PAIR 192 90. Thermal characteristics of louvered fins with a low-reynolds number flow 193 91. THERMAL EFFICIEnCY OF FIRE TUBE BOILER USED MAYAOHIN BIODIESEL AS A FUEL 194 92. Thermogravimetric analysis of giant sensitive plants under air atmosphere 195 93. UPGRADING OF LOW TEMPERATURE SOLAR HEAT FOR HIGHER TEMPERATURE APPLICATION AND COOLING 196 94. Use of oscillating heat pipe technique as extended surface in wire-on-tube heat exchanger for heat transfer enhancement 197 198 95. WATER QUALITY VARIATION IN A POND RAISING TILAPIA CAGE CULTURE Faculty of science 1. 2-[3-(2-Aminoethylsulfanyl)propylsulfanyl]ethanamine Bearing Dansyl Subunits: An Efficient, Simple, and Rapid Fluorometric Sensor for the Detection of Mercury(II) Ions 199 200 2. A Barium-Calcium Silicate Glass for Use as Seals in Planar SOFCs 3. A Hybrid Method for a Countable Family of Multivalued Maps, Equilibrium Problems, and Variational Inequality Problems 201 4. A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer 202 5. A Multiple Processing Hybrid Flow System for Analysis of Formaldehyde Contamination in Food 203 6. A new approximation method for common fixed points of a finite family of asymptotically quasi-nonexpansive mappings in Banach spaces 204 7. A New Approximation Method for Common Fixed Points of a Finite Family of Generalized Asymptotically Quasinonexpansive Mappings in Banach Spaces 205 8. A New Azwafluorenone from the Roots of Polyalthia cerasoides and its Biological Activity 206 9. A New General Iterative Method for a Finite Family of Nonexpansive Mappings in Hilbert Spaces 207 10. A role of BNLT compound addition on structure and properties of PZT ceramics 208 11. Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production 209 12. Age Hardening and Precipitation Behavior of an Experimental Cast Al-Mg-Si Alloy Treated by T6 and T6I6 Heat Treatments 210 ix 13. An iterative method for equilibrium problems and a finite family of relatively nonexpansive mappings in a Banach space 211 14. Analysis of lead molybdate and lead tungstate synthesized by a sonochemical method 212 15. Annotation and in silico localization of the Affymetrix GeneChip Porcine Genome Array 213 16. Anti-Cancer Compound Screening and Isolation: Coscinium fenestratum, Tinospora crispa and Tinospora cordifolia 214 17. Antioxidant and Anti-inflammatory Activities of Brown Marine Alga, Padina minor Yamada 215 18. AZO/Ag/AZO multilayer films prepared by DC magnetron sputtering for dye-sensitized solar cell application 216 217 19. Botanical notes 20. Calculation of Weighted Averages Approach for the Estimation of Ping Tolerance Values 218 21. Can leaf degrading enzymes provide evidence that endophytic fungi becoming saprobes? 219 22. Carboxymethyl Cellulose-Assisted Hydrothermal Synthesis of PbS with Nano- and Micro-Crystals 220 23. Changes in ferroelectric properties of lead indium niobate-lead titanate ceramics under compressive stress applied perpendicular to an electric field 221 24. Changes in the 2DE protein profiles of chilli pepper (Capsicum annuum) leaves in response to Fusarium oxysporum infection 222 25. Characterization of AgBiS(2) nanostructured flowers produced by solvothermal reaction 223 26. Characterization of Ba(0.77)Sr(0.23)TiO(3) powder prepared from an oxalate co-precipitation and an impregnation method 224 27. Characterization of Bi(2)S(3) with different morphologies synthesized using microwave radiation 225 28. Characterization of copper sulfide nanostructured spheres and nanotubes synthesized by microwave-assisted solvothermal method 226 29. Characterization of micro-crystalline lead tungstate with different morphologies produced by the sonochemical process 227 30. CHARACTERIZATION OF MULTIPOD CADMIUM SULFIDE NANOSTRUCTURES SYNTHESIZED BY AMINETHERMAL METHOD 228 31. Characterization of nanostructured ZnO produced by microwave irradiation 229 32. Characterization of SrCO(3) and BaCO(3) nanoparticles synthesized by sonochemical method 230 231 33. Clubionidae from Laos and Thailand (Arachnida: Araneae) 34. Comparison of different methods for determination of Pt surface site concentrations for supported Pt electrocatalysts 232 35. Comparison of gamma-aminobutyric acid production in Thai rice grains 233 x 36. Complexity of terms, superpositions, and generalized hypersubstitutions 234 37. 235 Comprehensive Two-Dimensional Gas Chromatography-Mass Spectrometry Analysis of Volatile Constituents in Thai Vetiver Root Oils Obtained by Using Different Extraction Methods 38. Compressive strength and microstructure of carbon nanotubes-fly ash cement composites 236 39. Compressive strength, microstructure and thermal analysis of autoclaved and air cured structural lightweight concrete made with coal bottom ash and silica fume 237 40. 238 Controlled Gd(2)O(3) nanorods and nanotubes by the annealing of Gd(OH)(3) nanorod and nanotube precursors and self-templates produced by a microwave-assisted hydrothermal process 41. Convergence Analysis for a System of Equilibrium Problems and a Countable Family of Relatively Quasi-Nonexpansive Mappings in Banach Spaces 239 42. Cultivation of Spirulina platensis using Anaerobically Swine Wastewater Treatment Effluent 240 43. Defect-free Poly(9,9-bis(2-ethylhexyl)fluorene-2,7-vinylene) for Polymer Light-Emitting Diode (PLED) Devices 241 44. Demiclosed Principle for Asymptotically Nonexpansive Mappings in CAT(0) Spaces 242 45. Determination of nitrofurans residues in animal feeds by flow injection chemiluminescence procedure 243 46. Dielectric and Ferroelectric Properties of Pb(Zr(1/2)Ti(1/2))O(3)-Pb(Ni(1/3)Nb(2/3))O(3) Ceramics Under Perpendicular Compressive Stress 244 47. Dielectric properties of lead-free solid solution of Bi0.487Na0.487La0.017TiO3 and BaTiO3 245 48. Dispersion of Particulate Matter (PM10) from Forest Fires in Chiang Mai Province, Thailand 246 49. Diversity of Arbuscular Mycorrhizal Fungi in Mimosa invisa and Effect of the Soil pH on the Symbiosis 247 248 50. Doped Soap Membranes Selectively Permeate a Chiral Isomer 51. Ecological status of the lowland deciduous forest in Chang Kian Valley, Chiang Mai, northern Thailand 249 52. Effect of compressive stress on the ferroelectric hysteresis behavior in 0-3 PZT-cement composites 250 53. Effect of electrolyzed oxidizing water and continuous ozone exposure on the control of Penicillium digitatum on tangerine cv. ‘Sai Nam Pung’ during storage 251 54. Effect of feeding Spirulina platensis on growth and carcass composition of hybrid red tilapia (Oreochromis mossambicus x O. niloticus) 252 55. Effect of Particle Size on Dielectric and Ferroelectric Properties of 0-3 Lead Magnesium Niobate Titanate-Portland Cement Composites 253 56. Effect of plasma treatment on hydrophobicity and barrier property of polylactic acid 254 xi 57. Effect of Si(3)N(4) nanoparticulates on the mechanical and electrical properties of PZT ceramics 255 58. Effect of solvents on the microstructure of CaWO(4) prepared by a solvothermal synthesis 256 59. Effect of Temperature on the Dielectric Properties of 0-3 PZT-Cement Composites 257 60. Effects of Beryllium on Microstructure and Resiliency of Silver-Copper Alloy 258 61. Effects of bromelain on cellular characteristics and expression of selected genes in canine in vitro chondrocyte culture 259 62. Effects of Mn and Cu Doping on Electrical Properties of 0.75BiFeO(3)-0.25BaTiO(3) Ceramics 260 63. Effects of Parallel and Perpendicular Compressive Stresses on the Dielectric and Ferroelectric Properties of Soft PZT Ceramics 261 64. Effects of vibro-milling on relaxor ferroelectric behavior and phase transition of lead-free Ba(Zr0.25Ti0.75)O3 ceramics 262 65. Effects of vibro-milling time on phase formation and particle size of Zn(3)Nb(2)O(8) nanopowders 263 66. Effects of ZnO Nanowhiskers Addition on Microstructure and Dielectric Properties of Lead Titanate Ceramics 264 67. Efficacy and Safety of 1-Month Postpartum Zidovudine- Didanosine to Prevent HIV-Resistance Mutations after Intrapartum Single-Dose Nevirapine 265 68. Electrical and Aging Properties of Doped Barium Titanate Ceramics 267 268 69. ELECTRICAL AND MECHANICAL PROPERTIES OF PZT/PVDF 0-3 COMPOSITES 70. Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films 269 71. Endophytic actinomycetes isolated from Aquilaria crassna Pierre ex Lec and screening of plant growth promoters production 270 72. Enhanced Photocurrent Generation in Nanostructured Chromophore/Carbon Nanotube Hybrid Layer-by-Layer Multilayers 271 73. Enhanced piezoelectric properties and lowered sintering temperature of Ba(Zr(0.07)Ti(0.93))O(3) by B(2)O(3) addition 272 74. Enhancement of Ethanol Sensing Properties by Alloying TiO(2) With ZnO Tetrapods 273 75. Enhancement of Ethanol Sensing Properties by Impregnating Platinum on Surface of ZnO Tetrapods 274 76. Enhancement of sensor response by TiO(2) mixing and Au coating on ZnO tetrapod sensor 275 77. Environmental and biological factors affect desiccation tolerance of algae from two rivers (Thailand and New Zealand) with fluctuating flow 276 78. Estrogen bioassy of Pueraria mirifica Airy Shaw and Suvatabandhu 277 xii 79. Ethanol-Promoted Fabrication of Tungsten Oxide Nanobelts with Defined Crystal Orientation 278 80. Extended X-Ray Absorption Fine Structure and X-Ray Diffraction Studies of Mn-Doped PZN-PZT Ceramics 279 81. Fabrication and Electrical Properties of Lead Zirconate Titanate-Cement-Epoxy Composites 280 82. Fabrication and properties of plasma-sprayed Al(2)O(3)/TiO(2) composite coatings: A role of nano-sized TiO(2) addition 281 83. Fallow to forest: Applying indigenous and scientific knowledge of swidden cultivation to tropical forest restoration 282 283 84. Fast Processing Technique for TiC Coatings on Titanium 85. Ferroelectric Hysteresis Behavior of 0-3 PMNT-Cement Composites 284 86. Fifty Three New Record Species of Benthic Diatoms from Mekong River and Its Tributaries in Thailand 285 87. Five new Mallinella species from the Sundaland of Indonesia (Araneae, Zodariidae) 286 287 88. Fixed point theorems for alpha-nonexpansive mappings 89. FIXED POINT THEOREMS FOR NONLINEAR MAPPINGS AND STRICT CONVEXITY OF BANACH SPACES 288 90. Flame-Spray-Made Undoped Zinc Oxide Films for Gas Sensing Applications 289 91. Flow based immuno/bioassay and trends in micro-immuno/biosensors 290 92. Flow Injection Determination of Iron Ions with Green Tea Extracts as a Natural Chromogenic Reagent 291 93. Flow injection spectrophotometric determination of lead using 1,5-diphenylthiocarbazone in aqueous micellar 292 94. Frequency dependence of the Ising-hysteresis phase-diagram: Mean field analysis 293 95. gamma-Aminobutyric Acid (GABA) Accumulations in Rice During Germination 294 96. Gel-carbon nanotube materials: the relationship between nanotube network connectivity and conductivity 296 97. Genetic diversity and community of endophytic actinomycetes within the roots of Aquilaria crassna Pierre ex Lec assessed by Actinomycetes-specific PCR and PCR-DGGE of 16S rRNA gene 297 98. Genetic evidence supports linguistic affinity of Mlabri-a hunter-gatherer group in Thailand 298 99. Graft polymerization of flame-retardant compound onto silk via plasma jet 300 100. Grain growth behavior in bismuth titanate-based ceramics 301 101. haracteristics of Trapiche Blue Sapphires from Southern Vietnam 302 102. Harmonic Analysis of Dynamic Hysteresis Respons of BaTiO3 Bulk Ceramics 303 103. High-performance thin-layer chromatographic determination of ketoconazole in pharmaceutical formulations 304 xiii 104. HRTEM and ADF-STEM of precipitates at peak-ageing in cast A356 aluminium alloy 305 105. Hydrothermal synthesis of double sheaf-like Sb(2)S(3) using copolymer as a crystal splitting agent 306 106. Hydrothermal Synthesis of Lead Titanate Fine Powders at Water Boiling Temperature 307 107. Hydrothermal synthesis of monoclinic WO(3) nanoplates and nanorods used as an electrocatalyst for hydrogen evolution reactions from water 308 108. Hysteresis scaling relations in polycrystalline BaTiO(3) bulk ceramics 309 109. Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities 310 110. Immunoproteomic Assay of Streptococcus suis Serotype 2 Isolated from Patients 311 111. Impedance study of giant dielectric permittivity in BaFe(0.5)Nb(0.5)O(3) perovskite ceramic 312 112. In vitro cultivation and fruit body formation of the black bolete, Phlebopus portentosus, a popular edible ectomycorrhizal fungus in Thailand 313 113. Infections of Nosema ceranae in four different honeybee species 314 114. Influence of B(2)O(3) on electrical properties and phase transition of lead-free Ba(Ti(0.9)Sn(0.1))O(3) ceramics 315 115. 316 Influence of metal cofactors and water on the catalytic mechanism of creatininase-creatinine in aqueous solution from molecular dynamics simulation and quantum study 116. Interfacial morphology and domain configurations in 0-3 PZT-Portland cement composites 317 117. Ishikawa Iterative Process for a Pair of Single-valued and Multivalued Nonexpansive Mappings in Banach Spaces 318 118. Large-scale synthesis of CuS hexaplates in mixed solvents using a solvothermal method 319 119. L-Asparaginase Production by Bipolaris sp BR438 Isolated from Brown Rice in Thailand 320 120. Luminescence and absorbance of highly crystalline CaMoO(4), SrMoO(4), CaWO(4) and SrWO(4) nanoparticles synthesized by co-precipitation method at room temperature 321 121. Mesoscale Simulation of a Very Heavy Rainfall Event over Mumbai, Using the Weather Research and Forecasting (WRF) Model 322 122. Microstructural, physical, and thermal analyses of Portland cement-fly ash-calcium hydroxide blended pastes 323 123. Microwave-assisted hydrothermal synthesis of Bi(2)S(3) nanorods in flower-shaped bundles 324 124. Microwave-assisted Pt-Co-Cr/C ternary compound preparation applied as a cathode catalyst for PEMFC 325 125. Microwave-assisted synthesis and characterization of SrMoO(4) and SrWO(4) nanocrystals 326 126. Molecular dynamics simulations of Krytox-Silica-Nafion composite for high temperature fuel cell electrolyte membranes 327 xiv 127. Molecular Markers for Identification of Stellantchasmus falcatus and a Phylogenic Study using the HAT-RAPD Method 329 128. Molecular Modeling of Peroxidase and Polyphenol Oxidase: Substrate Specificity and Active Site Comparison 330 129. Molecular simulations of ultra-low-energy nitrogen ion bombardment of A-DNA in vacuum 331 130. Monitoring of arsenic in aquatic plants, water, and sediment of wastewater treatment ponds at the Mae Moh Lignite power plant, Thailand 332 131. Monte Carlo Investigation of Ferromagnetic Properties Under Compressive Stress 334 132. Muscodor cinnamomi, a new endophytic species from Cinnamomum bejolghota 335 133. Nanostructured carbon nanotubes/copper phthalocyanine hybrid multilayers prepared using layer-by-layer self-assembly approach 336 134. Oblongolides from the Endophytic Fungus Phomopsis sp BCC 9789 337 135. Occurrence and diversity of basidiomycetous endophytes from the oil palm, Elaeis guineensis in Thailand 338 136. Patterns of occurrence of myxomycetes on lianas 339 137. Pervaporation flow injection analysis for the determination of sulphite in food samples utilising potassium permanganaterhodamine B chemiluminescence detection 340 138. Phase development and dielectric responses in PMN-BNT ceramics 341 139. Phase transition anomaly observed in PbZrO3/Al2O3 nanocomposites 342 140. Phase transition behavior in (1-x)PZT-xBiAlO3 ceramics 343 141. Phenolic content and antioxidant properties of green chilli paste and its ingredients 344 142. Plasma immersion low-energy-ion bombardment of naked DNA 345 143. Polyethyleneimine/sodium dodecyl sulphate adsorbed silica particles and their adsorption properties 346 144. Polymer-assisted hydrothermal synthesis of Bi(2)S(3) nanostructured flowers 347 145. Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach 348 146. Processing and properties of nanoporous hydroxyapatite ceramics 349 147. Production of Monoclonal Antibody to Acaricide Dicofol and Its Derivatives 350 148. Quantitative analysis of a complex metal carbide formed during furnace cooling of cast duplex stainless steel using EELS and EDS in the TEM 351 149. Randomized controlled trial of live lactobacillus acidophilus plus bifidobacterium bifidum in prophylaxis of diarrhea during radiotherapy in cervical cancer patients 352 150. Remarks on Asymptotic Centers and Fixed Points 353 151. Reverse flow injection spectrophotometric for determination of aluminium (III) 354 xv 152. SCHUADER’S CONJECTURE ON CONVEX METRIC SPACES 355 153. Selection of Starter Cultures for the Production of Vegetarian Kapi, a Thai Fermented Condiment 356 154. Self-Reduction of Gold on Activated Carbon Cloth 357 155. Sensor response formula for sensor based on ZnO nanostructures 358 156. Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species 359 157. Sequential Injection-Cation Exchange Micro-column System for Hemoglobin Typing to Differentiate HbE Carriers 360 158. Sequential Injection-Immunoassay System with a Plain Glass Capillary Reactor for the Assay of Hyaluronan 361 159. Short Communication: Hematological Safety of Perinatal Exposure to Zidovudine in Uninfected Infants Born to HIV Type 1-Infected Women in Thailand 362 160. Simulating Pesticide Transport from a Sloped Tropical Soil to an Adjacent Stream 364 161. Sorption Characteristics of Indigo Carmine as a Blue Colorant for Use in One-bath Natural Dyeing 365 162. Spatial and temporal variations of ambient PM(10)-bound polycyclic aromatic hydrocarbons in Chiang Mai and Lamphun Provinces, Thailand 366 163. Spatial and Temporal Variations of Physical-Chemical Water Quality and some Heavy Metals in Water, Sediments and Fish of the Mae Kuang River, Northern Thailand 367 164. Spray Forming of Bulk Ultrafine-Grained Al-Fe-Cr-Ti 368 165. Stemphol Galactoside, a New Stemphol Derivative Isolated from the Tropical Endophytic Fungus Gaeumannomyces amomi 369 166. Stress-dependent dielectric and ferroelectric properties of sol-gel-derived PZT/WO(3) ceramics 370 167. Strong convergence of a new iterative scheme for a finite family of strict pseudo-contractions 371 168. Strong convergence of composite iterative schemes for a countable family of nonexpansive mappings in Banach spaces 372 169. Strong Convergence Theorem for a New General System of Variational Inequalities in Banach Spaces 373 170. STRONG CONVERGENCE THEOREMS BY HYBRID METHODS FOR FAMILIES OF RELATIVELY NONEXPANSIVE MAPPINGS IN HILBERT SPACES 374 171. Strong convergence theorems for a general system of variational inequality problems, mixed equilibrium problems and fixed points problems with applications 375 172. STRONG CONVERGENCE THEOREMS FOR MAXIMAL MONOTONE OPERATORS AND GENERALIZED NONEXPANSIVE MAPPINGS IN BANACH SPACES 376 173. Sub-coercive field dynamic hysteresis in morphotropic phase boundary composition of Pb(Zr(1/2)Ti(1/2))O(3)-Pb(Zn(1/3)Nb(2/3))O(3) ceramic and its scaling behavior 377 xvi 174. Sulfur hexafluoride plasma surface modification of Gly-Ala and Ala-Gly as Bombyx mori silk model compounds: Mechanism investigations 378 175. Surface Characterization of PZN Ceramics Prepared by a Columbite B-Site Precursor 379 176. Synthesis of a Novel Fluorescent Sensor Bearing Dansyl Fluorophores for the Highly Selective Detection of Mercury (II) Ions 380 177. Synthesis of CdIn(2)Se(4) compound used as thermoelectric materials via the solution method 381 178. Synthesis of hexagonal WO(3) nanowires by microwave-assisted hydrothermal method and their electrocatalytic activities for hydrogen evolution reaction 382 179. Synthesis of nanocrystalline metal molybdates using cyclic microwave radiation 383 180. Synthesis of novel ZnS/ZnAl(2)S(4) core/shell nanocomposites using a facile solvothermal route 384 181. Synthesis of zeolite phases from combustion by-products 385 182. Synthesis, characterisation and photoluminescence of nanocrystalline calcium tungstate 386 183. Taxonomic characterization of Streptomyces strain CH54-4 isolated from mangrove sediment 387 184. The case for the use of unrefined natural reagents in analytical chemistry-A green chemical perspective 388 185. The chemical composition and antioxidant activities of basil from Thailand using retention indices and comprehensive two-dimensional gas chromatography 389 186. The Debye Dielectric Behavior of Mixed Normal and Relaxor-Ferroelectrics: Monte Carlo Investigation 390 187. The effect of H(2)O and PEG on the morphologies of ZnO nanostructures synthesized under microwave radiation 391 188. The Effects of Sugar Application on the Concentrations of Anthocyanin and Flavonol of ‘Mahajanaka’ Mango (Mangifera indica Linn. cv. Mahajanaka) Fruit 392 189. The Fixed Point Property of Unital Abelian Banach Algebras 393 190. The gas phase conformers and vibrational spectra of valine, leucine and isoleucine: An ab initio study 394 191. The Interactive Effects of Inorganic Carbon and Light on a Tropical Submerged Macrophyte: Egeria densa Planchon (Hydrocharitaceae) 395 192. The occurrence of Melissococcus plutonius in healthy colonies of Apis mellifera and the efficacy of European foulbrood control measures 396 193. The solution route synthesis of the Cu(0.5)Ag(1.5)InSe(3) compound for thermoelectric applications 397 194. The spitting spider family Scytodidae in Thailand, with descriptions of three new Dictis species (Araneae) 398 195. Theoretical Description of Electromagnetic Nonbonded Interactions of Radical, Cationic, and Anionic NH(2)BHNBHNH(2) Inside of the B(18)N(18) Nanoring 399 xvii 196. Theoretical Study of the Bombyx mori Silk Surface Functionalization: Quantum Mechanical Calculation of the Glycine-Alanine Unit Reacting with Fluorine and Molecular Dynamic Simulation of Wettability 401 197. Thermal analysis and microstructure of Portland cement-fly ash-silica fume pastes 402 198. Thermal expansion behavior and estimated total polarizations of lead zirconate titanate-lead nickel niobate ceramics 403 199. Thermal sprayed stainless steel/carbon nanotube composite coatings 404 200. TPA-ZrO(2)-SiO(2)-Catalyzed Polyesterification of TMP Polyester 405 201. Tris(ethylenediamine) cobalt(II) sulfate 406 202. TWO-STEP SYNTHESIS OF CdS (hcp) NANORODS USING SONOCHEMICAL AND HYDROTHERMAL PROCESSES 407 203. Utilization of ceramic waste as fine aggregate within Portland cement and fly ash concretes 408 204. Utilization of fly ash with silica fume and properties of Portland cement-fly ash-silica fume concrete 409 205. Weak and strong convergence theorems for a finite family of generalized asymptotically quasi-nonexpansive mappings 410 206. Weak Convergence Theorems for a Countable Family of Strict Pseudocontractions in Banach Spaces 411 207. Webcam camera as a detector for a simple lab-on-chip time based approach 412 208. yclic microwave assisted synthesis of Sb(2)S(3) dumb-bells using polyvinylpyrrolidone as a template and splitting agent 413 209. ZnO Nanobelts as a Photoelectrode for Dye-Sensitized Solar Cell Faculty of social sciences 414 1. Artwork and Community History around Burmese-Tai Yai Temples in Lampang 415 2. Prehistoric Culture of Pra Tu Pha Limestone Hills in Mae Moh District, Lampang Province 416 Postharvest technology innovation center 1. BrimA, total Acidity and total soluble solid correlate to total carotenoid contents as indicators of the ripening process of six Thai mango fruit cultivars 417 2. Comparative Characterisation of Durian, Mango and Avocado 418 3. Diversity of Moulds in Fresh Longan 419 4. Drying Kinetics and Dried Product Quality of Rosemary Leaves, Lavender Flowers and Rose Petals 420 5. Effect of electrolyzed oxidizing water and continuous ozone exposure on the control of penicillium digitatum on tangerine cv. ‘Sai Nam Pung’ during storage 421 6. Effect of Hydrogen Peroxide on the Quality of Abalone Mushroom 422 7. Effect of Radio Frequency Heat Treatment to Control Aspergillus flavus in Maize (Zea mays) 423 xviii 8. Inhibitory Effect of Titanium Dioxide (TiO2) Photocatalytic Ozonation on Controlling Anthracnose Disease of Bird Chilli (Capsicum fruescens L.) After Harvest 424 9. PCR-based Detection and Differentiation of Anthracnose Pathogen isolates from ‘Nam Dok Mai Si Thong’ Mang Orchards at Prao, Chiang Mai 425 10. The Decrease of Quiescent and Anthracnose Disease for Increasing Export Potential of “Nam Dok Mai Si Thong” mango at Prao in Chiang Mai 426 II. The Abstracts Presented in the conferences Faculty of Dentistry 1. CORRELATION OF DELIVERY MODES AND EARLY CHILDHOOD CARIES 429 Faculty of Agro-industry 1. CRUDE MALVA NUT GUM AFFECTS PASTING AND TEXTURAL PROPERTIES OF WHEAT FLOUR IN THE PRESENCE OR ABSENCE OF SODIUM CHLORIDE 431 2. DEVELOPMENT OF COCONUT MILK ICE CREAM MIXED WITH MALVA NUT JELLY 432 433 3. DEVELOPMENT OF GLUTEN-FREE BREAD MIXED WITH JIAOGULAN 4. EFFECT OF CRUDE MALVA NUT GUM ON PASTING BEHAVIOR AND FREEZE-THAW STABILITY OF TAPIOCA STARCH 434 5. EFFECT OF SOAKING CONDITIONS ON PHYSICOCHEMICAL PROPERTIES OF RED-JASMINE GERMINATED BROWN RICE FLOUR 435 6. PASTING BEHAVIOR AND FREEZE-THAW STABILITY OF WHEAT, RICE AND GLUTINOUS RICE FLOURS MIXED WITH MALVA NUT GUM SYSTEMS 436 7. QUALITY PARAMETERS AND GLYCEMIC INDEX OF GLUTEN-FREE COOKIE USING DIFFERENT SWEETENERS 437 faculty of engineering 1. A Novel Neuro-Fuzzy Method for Linguistic Feature Selectionand Rule-Based Classification 438 2. Application of Cleaner Technology and Experimental Design for the Reduction of Chemical Substance Consumption in the Hard Disk Drive Arm Coil Assembly Process 439 3. Application of Cleaner Technology Concepts in the Arm Coil Assembly Process of Hard Disk Drive Manufacturing 440 4. Application of Fuzzy Analytic Hierarchy Process for Supplier Selection of Automobile and Electronic Industries 441 5. Automatic Detection of Electricity Pylons in Aerial Video Sequences 442 6. Bus Stop Selection for Bus System of Large IndustrialFactory 443 7. Characterization on Properties of Modification Gelatin Films with Carboxymethylcellulose 444 8. Design of Signal Control by Using FPGA for Indirect X-Ray Detector Couple CIS Image Sensor 445 9. Development of Wireless Videonystagmography Recording for Medical Diagnosis 446 447 10. Drying kinetics of Longan Flesh at High Temperature xix 11. DYNAMIC BEHAVIOR OF ELECTROCNIC CABINET 448 12. EARTHQUAKE LOSS ESTIMATION FOR CHIANG MAI CITY 449 13. Effect of DSM in Cold Storage on Product Properties and Electricity Usage 450 14. Effect of Powder Sized on Heat Transfer Characteristics of Miniature Sintered-Wick Heat Pipe 451 452 15. EFFECTS OF SEISMIC FORCE ON MAE THALOP LUANG DAM 16. Experimental Investigation of Closed Loop Oscillating Heat Pipe as the Condenser for Vapor Compression Refrigeration 453 17. Impact of Work-Rest Period on Mental Fatigue in Inspection Task with Microscope: Case Study of Hard Disk Drive Component Manufacturing Company 454 18. Modeling and Validation of a Single-Cell PEMFC 455 19. Noise covariance estimation using dual estimation for disturbance storm time index application 456 20. PERFORMANCE COMPARISON OF HEAT PUMP DRYER FOR SMALL INDUSTRY BY DIFFERENT CONTROL METHODS OF HOT AIR TEMPERATURE 457 21. Power Transfer Capability Enhancement with Multitype FACTS Controllers Using Particle Swarm Optimization 458 22. PREDICTION OF INITIAL NATURAL DETERIORATION OF HISTORICAL BRICK MASONRY STRUCTURES BY ACCELERATION TEST USING SULFURIC ACID 459 23. PRORISK: A Project Oriented Risk Management Model 460 24. SEISMIC EVALUATION OF SCHOOL BUILDINGS IN CHIANG MAI 461 25. Space Vector Pulse Width Modulation (SVPWM) of Two-Level Inverter Using TMS320F2812 Digital Signal Processor (DSP) 26. The Assessment of Carbon Dioxide Elasticity by Using the Input-output Method: The case of Thailand faculty of fine arts 1. The Furniture Design Research by Creating with contemporary Cultural Value for Value Added and Market Differentiated 462 463 464 energy research and development institute-nakornping 1. Biomethane: An Alternative Green Fuel to CNG 465 Faculty of humanities 1. Participation of the Communities to Process Food in GHP / GMP Guidlines : A Case Study of Red Chili Paste and Tua Noa Products Community Enterprise 466 iii. The Abstracts unpublished faculty of associated medical sciences 1. DETERMINATION OF ORGANOCHLORINE PESTICIDES LEVEL IN FARMER SERA FROM CHIANG MAI PROVINCE 469 2. DEVELOPMENT OF THE OCCUPATIONAL PERFORMANCE EVALUATION FOR DEMENTED ELDERLY 470 xx 3. PREVALENCE OF OBESITY AND COMPARISON OF THE CARDIOVASCULAR FITNESS IN FEMALE UNDERGRADUATE STUDENTS, FACULTY OF ASSOCIATED MEDICAL SCIENCES, CHIANG MAI UNIVERSITY 471 4. QUALITY OF LIFE AND PERCEIVED HEALTH STATUS AMONG PEOPLE WITH SPINAL CORD INJURY 472 5. THE RESULTS OF THAI ELEPHANT-ASSISTED THERAPY PROGRAM ON BASIC SENSORY-MOTOR PERFORMANCE AND ADAPTIVE BEHAVIOR IN INDIVIDUALS WITH AUTISM: A PILOT STUDY 473 6. USE OF INTERNET FOR EDUCATION OF OCCUPATIONAL THERAPY STUDENTS OF THE FACULTY OF ASSOCIATED MEDICAL SCIENCES, CHIANG MAI UNIVERSITY 474 faculty of dentistry 1. ALTERED EXPRESSION OF P-AKT, AKT (3 ISOFORMS), MMP, VIMENTIN AND E-CADHERIN IS ASSOCIATED WITH EPITHELIAL- MESENCHYMAL TRANSITION IN ORAL SQUAMOUS CELL CARCINOMA 476 2. ANTI-INFLAMMATORY EFFECT OF THE PURIFIED ACTIVE CHEMICALS FROM BOESENBERGIA PANDURATA ROXB. (THAI GINGER, FINGERROOT, OR GKRA-CHAI) IN CULTURED HUMAN ORAL CELLS 477 3. DEVELOPMENT OF NEWLY REGENERATE DENTOALVEOLAR BONE WITH TWIN-TRACK DISTRACTION COMBINED WITH MINISCREW IMPLANTS 479 4. EFFECTS OF DIFFERENT PRIMERS AND ADHESIVE RESINS ON SHEAR BOND STRENGTH OF ORTHODONTIC BRACKET 480 5. IDENTIFICATION OF PREVOTELLA BARONIAE BY POLYMERASE CHAIN REACTION (PCR) 481 6. RESONANCE FREQUENCY ANALYSIS OF ORTHODONTIC MINISCREWS SUBJECTED TO IMMEDIATE OR DELAYED LOADING 482 faculty of nursing 1. EFFECTS OF USING AN INTERNET-BASED PROGRAM TO PROMOTE HEALTHY EATING BEHAVIOR AMONG THAI EARLY ADOLESCENTS 483 2. LESSON LEARNED FROM CHILDCARE CENTER MODEL IN FACULTY OF NURSING, CHIANG MAI UNIVERSITY 484 3. NORMALCY FROM THE PERSPECTIVES OF CHRONICALLY ILL CHILDREN, FAMILIES, AND NURSING PERSONNEL 486 488 4. ROLE MODEL BEHAVIORS OF NURSING FACULTY MEMBERS IN THAILAND 5. THAI NURSING STUDENTS’ ADAPTION TO PROBLEM-BASED LEARNING: A QUALITATIVE STUDY 489 6. WOMAN SEXUAL RIGHTS AS PERCEIVED BY YOUNG ADULT MEN IN CHIANG MAI, THAILAND 490 faculty of architecture 1. Architecture Building Facilities Management 2. Performance of Chimney Techniques: Case of Chiang Mai House 491 492 faculty of engineering 1. Application of Lean Thinking for Waste Reduction in Hard Disk Drive Manufacturing 493 xxi 2. Continuous Combined Infrared and Hot Air Dryer for Laboratory Scale 494 social research institute 1. DATABASE FOR LAND USE PLANNING IN CHANGPUAK MUNICIPALITY 495 2. The Knowledge Management in Lan Na Traditional Medicine: A Case Study of Mareng (Baheng) Illness. 496 497 3. URBAN PLANNING PRACTICE TO SUPPORT CHIANG MAI CITY RENEWAL facutly of fine arts 1. Drawing creation of human forms inspired by line colour Form and texture in ceramic art 498 2. Hill Tribes; Beliefs and Ways of Life towards the Creation of Contemporary Sculpture 499 3. SUSTAINABLE EFFICACIOUS THE RENOVATION OF HUMAN MIND 501 4. Ta-Lung of Modern Technology 503 5. Transforming Chiang Mai: Creative Photography by Plastic Camera Holga 504 facutly of law 1. Laws and Custom in the Management of Natural Resources: Comparative Case Studies of the Karen in Mae Wang District and the Community Forests Management of Chiang Dao District, Chiang Mai Province faculty of political sciences 505 1. Project on Empowering Community to Combat Smog Crisis: A Research Study for Alternative Solutions to Smog Problems and Watching Network Development 506 2. Project on Empowering Community to Combat Smog Crisis: Community Development Activities to Build Solution Learning Sites within Communities 507 3. 508 The Changes of Working Procedures and Public Service Delivery Process of Regional Public Organizations in Chiang Mai Province to Comply with the Policy on Citizen-Centered Governance college of arts, media and technology 1. The Factors affecting tourist decisions in services selection of Boutique Hotels in Chiang Mai Province 510 AuthorS Index 511 xxii the abstracts of full papers publication GROWTH AND DEVELOPMENT OF PRESCHOOL CHILDREN IN CHILD DAY CARE CENTERS IN CHIANG MAI Sakda Pruenglampoo1, Posri Leelapat1, Chamnong Kingkeow1, Chatchai Muenkonkeaw1, Anong Suntranon2, Srisuda Sae-tang2, Ampun Chaitongsri2, Molwipa Sirihorachai2, Surasing Visrutaratna2 and Ampica Mangklabruks1 1 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Chiang Mai Provincial Public Health Office, Chiang Mai 50200, Thailand. 2 ABSTRACT Growth is a marker of health and nutrition status. Well growth during childhood leads to well physical and mental development in later life. This study aimed to assess growth and development in 2,345 preschool children aged 3 to less than 6 years in 96 child day care centers in seven districts of Chiang Mai province namely: Mueang, Hang Dong, San Kam Phaeng, Chiang Dao, Doi Tao, Om Koi and Samoeng. Growth was assessed by weight (wt) and height (ht) while development of preschool children was assessed in 5 areas: Gross Motor (G.M.), Fine Motor (F.M.), Receptive Language (R.L.), Expression Language (E.L.) including Personal and Social (P.S.). The results showed that the average wt and ht of preschool children of Om Koi, Doi Tao, and Samoeng were lower than those of other districts while Om Koi showed the lowest values. Using wt for age and ht for age as indices, Om Koi had the most percentage of preschool children below cut off point. Using wt for ht as an index, Hang Dong showed the most percentage of preschool children below cut off point. Regarding to development, it was found that Om Koi had more percentage of children with delayed development in F.M., E.L. and P.S. compared with other districts. Samoeng and Hang Dong had quite the same percentage of children with delayed development in G.M. and R.L. Samoeng also had quite the same percentage of children with delayed development in F.M. as Om Koi. It seemed that lower wt and ht was one factor which may increase risk of the under development of children in Om Koi. Published in the Proceeding of the 4th Thailand Congress of Nutrition, Sep. 2010, p. 231. Presented poster at the 4th Thailand Congress of Nutrition, Sep. 2010. Supported by Thai Government in fiscal year 2008. 3 IODINE STATUS OF PRESCHOOL CHILDREN IN CHILD DAY CARE CENTERS IN CHIANG MAI Sakda Pruenglampoo1, Posri Leelapat1, Chamnong Kingkeow1, Chatchai Muenkonkeaw1, Anong Suntranon2, Srisuda Sae-tang2, Ampun Chaitongsri2, Molwipa Sirihorachai2, Surasing Visrutaratna2 and Ampica Mangklabruks1 1 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Chiang Mai Provincial Public Health Office, Chiang Mai 50200, Thailand. 2 ABSTRACT Almost 90% of absorbed iodine in the body excretes in urine. So the excretion of iodine in urine is a good measure of iodine intake and reflects iodine status. This study aimed to assess iodine status using urinary iodine as indicator in 2,345 preschool children in 96 child day care centers in 7 districts of Chiang Mai province namely: Mueang, Hang Dong, San Kam Phaeng, Chiang Dao, Doi Tao, Om Koi and Samoeng. Urinary iodine concentration was analyzed using ammonium persulphate digestion and intensity of complex was measured by microplate reader. World Health Organization (WHO) criteria were used in the evaluation. The median urinary iodine concentration (UI) of 2,345 children was 217.80 ug/L indicated that they had adequate iodine intake the same as the children in the other 6 districts except Om Koi. The range of median UI of children in 6 districts was 171.39 ug/L (in Chiang Dao) to 240.07 ug/L (in Mueang district). The children in Om Koi had the median UI of 112.80 ug/L which above the cut off point of 100 ug/L. However, when consider % of children in Om Koi who had UI less than 50 ug/L, it was found to be 21.20% which was higher than 20% as additional criteria of WHO. It could be concluded that among 7 districts, there was still iodine deficiency in children of Om Koi whereas iodine intake was sufficient in other areas. 4 Published in the Proceeding of the 4th Thailand Congress of Nutrition, Sep. 2010, p. 232. Presented poster at the 4th Thailand Congress of Nutrition, Sep. 2010. Supported by Thai Government in fiscal year 2008. A PROPHYLACTIC EFFECT OF MASSAGE ON SYMPTOMS OF MUSCLE DAMAGE INDUCED BY ECCENTRIC EXERCISE OF THE WRIST EXTENSORS Peanchai Khamwong, Ubon Pirunsan and Aatit Paungmali Department of Physical Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The main purpose of this study was to determine the prophylactic effect of massage on symptoms of muscle damage induced by eccentric exercise of the wrist extensors. Twenty-eight healthy males, mean age of 20.8±1.2 yrs, participated in this study. A randomized experimental-controlled design was employed. Half of the participants were randomly divided into the massage and the control groups. Massage was used before eccentric exercise induction in the wrist extensor. The exercises were conducted on the non-dominant arm by using an isokinetic device. All subjects were tested to examine muscle damage characteristics including sensory-motor functions. Sensory-motor functions includes pain intensity [Visual Analogue Scale (VAS) and Modified Likert scale (LS), thermal pain threshold [cold pain (CPT)], and mechanical: pressure pain threshold (PPT), range of motion in active wrist flexion (ROM-AF) and extension (ROM-AE), range of motion in passive wrist flexion (ROM-PF) and extension (ROM-PE), grip strength (GS), and wrist extension strength (WES) at baseline, immediate and from 1st to 8th days after the exercise-induced muscle damage. Published in Journal of Sports Science and Technology, 2010; 10(1): 245-259. 5 A PROPHYLACTIC EFFECT OF PROPRIOCEPTIVE NEUROMUSCULAR FACILITATION (PNF) STRETCHING ON SYMPTOMS OF MUSCLE DAMAGE INDUCED BY ECCENTRIC EXERCISE OF THE WRIST EXTENSORS Peanchai Khamwong, Ubon Pirunsan and Aatit Paungmali Department of Physical Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Stretching with proprioceptive neuromuscular facilitation (PNF) is frequently used before exercise. The prophylactic effect of PNF on symptoms of muscle damage induced by eccentric exercise of the wrist extensors was examined in this study. Twenty-eight healthy males were randomly divided into the PNF group (n = 14) and the control group (n = 14). PNF was used before eccentric exercise induction in the wrist extensors. All subjects were tested to examine muscle damage characteristics including sensory-motor functions at baseline, immediate and from 1st to 8th days after the exercise-induced muscle damage (EIMD). The results demonstrated that the PNF group showed a lesser deficit in some sensory-motor functions (p < 0.05) than the control group. The prior PNF stretching application could be useful for attenuating the signs and symptoms of muscle damage after eccentric exercise. Published in Journal of Bodywork & Movement Therapies, 2010; in-press (doi:10:1016/ j.jbmt.2010.07.006). 6 EFFECTS OF UP-AND DOWNSLOPE WALKING ON MEAN AND VARIABILITY OF GAIT PARAMETERS IN ELDERLY WOMEN Sirinun Boripuntakul and Somporn Sungkarat Department of Physical Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Background: In daily living, we often encounter challenge situations such as negotiating uneven and inclined walking surfaces. It has been reported that irregular walking surfaces are highly associated with falls in elders. Nevertheless, little evidence has been directed towards the examination on how elders’ walking patterns are modified in these circumstances. Objective: To investigate the effects of up-and downslope walking on mean and variability of gait parameters in healthy elderly women. Method: Fifteen healthy elderly women with no history of fall walked on a treadmill at their preferred speed for 10 min in each testing condition. There were three testing conditions; 1) walked on level surface, 2) walk up 9°- slope and 3) walk down 9°- slope. Gait parameters and gait variability were captured using a 2-D motion analysis system then analyzed with a SiliconCoach program. Repeated measures ANOVA were conducted to determine the differences of each dependent variable among the three testing conditions. Results: Compared to the level surface, a significant increase in toe clearance and decrease in step length were observed in the 9° upslope walking condition. For the 9° downslope walking condition, a significant decrease in step length and maximal sole inclination were observed. Double-support time and gait variability were unchanged across the three testing conditions. Conclusion: Healthy, elderly women demonstrated a significant decrease in step length and increase in toe clearance while walking on the upslope surface. It was speculated that these changes reflected a cautious gait strategy the elders employed to ensure gait stability. Walking on the downslope surface resulted in a significant decrease step length and maximal sole inclination while other variables were unchanged. Stride length and stride time variability were relatively unchanged in the present study, suggesting efficiency of the neuromuscular control system in regulating gait and maintaining a steady walking pattern in healthy elders. Key words: Gait parameter, Gait variability, Upslope, Downslope, Elderly women Published in Thai Journal of Physical Therapy, 2010; 32(2), 55-61. 7 IMPACT OF FOOTWEAR STYLES ON POSTURAL CONTROL ABILITY OF FEMALE ELDERLY Supanee Chounchay, Somporn Sungkarat and Patima Silsupadol Department of Physical Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purposes of this study were: (1) to identify the top three footwear styles commonly worn by Thai elderly women and (2) to investigate the impact of footwear styles on postural control of Thai elderly women. The survey study was conducted on 170 Thai elderly women who lived in the area outside municipal limits, Chiang Mai province. The top three footwear styles obtained from the survey study were then used to investigate their impact on postural control. Thirty elderly women (mean aged = 63.23±3.22 yrs) participated in the second part of the study. All participants underwent the postural control tests while wearing each of the four footwear styles (i.e. sandal, thong, high-heel, and athletic shoes). The postural control tests used in the study were the one leg stance test (OLST), reach test, modified clinical test of sensory interaction and balance (mCTSIB), 10-meter walk test (TMW), and timed up and go test (TUG). One-way repeated measure analysis of variance (ANOVA) and Bonferroni was used to determine significant differences of the postural control ability among the four footwear styles. Findings showed that the top three footwear styles worn by Thai elderly women who lived outside municipal limits were sandal (45.3%), followed by thong (28.8%), and high-heel shoes (12.4%). Overall, there were no significant differences of all dependent variables among the 4 footwear styles except for the mCTSIB on foam surface with eye closed condition and TUG. Participants received higher score for the mCTSIB on foam surface with eye closed condition when they wore athletic shoes as compared to sandal (p= .02) and high-heel shoes (p= .05). For TUG, they walked faster when they wore athletic shoes compared to high-heel shoes (p= .04). In conclusion, the most popular footwear styles worn by Thai elderly women who live outside municipal limits were sandal, followed by thong, and high-heel shoes. Overall, the impact of each footwear style on postural control was not different with the exception for the modified CTSIB on foam surface with eye closed condition and the TUG in which the athletic shoes provide slightly better postural control than high heel and sandal. Key words: footwear styles, postural control ability, elderly women 8 Published in Thai Journal of Gerontology and Geriatric Medicine, 2010; 11(1), 25-35. RELIABILITY OF MUSCLE FUNCTION AND SENSORY PERCEPTION MEASUREMENTS OF THE WRIST EXTENSORS Peanchai Khamwong1, Kazunori Nosaka2, Ubon Pirunsan1 and Aatit Paungmali1 1 Department of Physical Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Exercise, Biomedical and Health Sciences, Edith Cowan University, Joondalup, Western Australia, Australia. ABSTRACT This study determined the reliability of muscle function and sensory perception measures of the wrist extensors. The test-retest reliability of the measurements was determined by an Intraclass correlation coefficient (ICC), coefficient of variation (CV), standard error of measurements (SEMs), and one-way repeated measures ANOVA using the values collected from 25 young (20.6±1.3 years) healthy male volunteers on two occasions separated by 1 day. The measures consisted of grip strength, wrist extension strength (WES), range of motion in active and passive wrist flexion and extension, choice reaction time, vibration sense (VIB), joint position error sense (JPE), cold pain (CP) and heat pain threshold, and pressure pain threshold. An acceptable reliability was determined as the ICC values greater than 0.85, CV less than 15%, and SEMs less than 5%. ICC of all measures except for JPE were greater than 0.85, only CV of JPE, CP and VIB exceeded 15%, SEMs were higher than 5% only for JPE and CP, and the ANOVA showed a significant time effect for CRT and WES. It is concluded that the most of the measurements except JPE are reliable and can be used to investigate effects of a physiotherapy intervention on the wrist extensors. Published in Physiotherapy Theory and Practice, 2010; 26 (6), 408-415. 9 SENSORY PROFILE ASSESSMENT TOOL FOR CHILDREN 0-36 MONTHS OF AGE, THAI VERSION Supaporn Chinchai and Sarinya Sriphetcharawut Department of Occupational Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this developmental research was to develop the sensory profile assessment tool for assessing sensory processing of children from birth to 36 months old in Thai version and to examine its psychometric properties; content validity and reliability. The Sensory Profile was translated from original version to target version by the researchers. Two experts in occupational therapy conducted the translation-back translation process on the target version and then examined for its content validity evidence by three occupational therapists who had at least 3 years using the sensory integrative approach for their clinical practice. The examination of 2 types reliability; internal consistency and test-retest reliability, was conducted in parents of 30 infants with birth to 6 months of age and 30 children with 7 to 36 months of age. These parents were recruited from the well baby clinics of Maharaj Nakorn Chiang Mai Hospital and Nakornping Hospital. The panel experts resulted in the establishment of content validity of the Sensory Profile Assessment Tool for Children from Birth to 36 Months Old, Thai Version with the index of item Objective Congruence (IOC) over 0.5 of all items. The analysis of Cronbach’s alpha coefficient revealed an acceptable internal consistency of the Sensory Profile Assessment Tool for Children from Birth to 6 Months Old, Thai Version in all areas (α = .74) and of the Sensory Profile Assessment Tool for Children from 7 to 36 Months Old, Thai Version in all areas (α = .92). Using an intraclass correlation coefficient (ICC) to examine test-retest reliability, the Sensory Profile Assessment Tool for Children from Birth to 6 Months Old, Thai Version had shown an excellent test-retest reliability (ICC = .92) and the Sensory Profile Assessment Tool for Children from 7 to 36 Months Old, Thai Version had demonstrated an excellent test-retest reliability (ICC = .89). 10 Published in Bulletin of Chiang Mai Associated Medical Science, 2010; 43 (3), 201-210. USING WEIGHTED VEST TO INCREASE ATTENTION IN CHILDREN WITH HYPERACTIVITY Nuntanee Satiansukpong, Supaporn Chinchai and Busabongkoch Chawengchao Department of Occupational Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this study was to examine the effectiveness of weighted vest whether it could increase an attending time in 12 students with hyperactivity, ages 3–15 years old from Kawila Anukul School, Muang Distric of ChiangMai. The ABA research design was used. The study was conducted in the 6 week period. In the first 2 weeks: the baseline phase, a child did not wear the weighted vest and then in the third and forth week: the intervention phase, a child wore the weighted vest, which was calibrated at 4 percent of the child’s body weight, for 30 minutes, 3 times a week. In the fifth and the sixth week: the withdrawn phase, a child was no longer used the weighted vest. The child’s attending time, distraction time were measured while doing table-top activity for 15 minutes after wearing the weighted vest for at least 10 minutes. The measurement was done 3 times a week, at the same time and on the same day in each week. The results from descriptive analyses showed that wearing weighted vest increased attending time in 2 cases of children with Mental Retarded (100%), in 2 cases of 3 children with Down’s syndrome (66.66%), in 2 cases of 7 children with Autism (28.58%). In conclusion wearing the weighted vest while doing table-top activity could increase attending time in 6 cases of 12 children with hyperactivity (50%). The distraction times in these cases also reduced. An occupational therapist may consider using weighted vest in hyperactive children with Mental Retardation and with Down’s syndrome. To use the weighted vest in hyperactive children with Autism may need further studies concerning suitability about the size, the frequency, and the duration of weighted vest for each case of children with Autism due to the varieties of symptoms. Published in Journal of Occupational Therapist Association of Thailand, 2010; 15 (3), 1-7. 11 A 3-YEAR FOLLOW-UP OF ANTIBODY RESPONSE IN HIV-INFECTED CHILDREN WITH IMMUNE RECOVERY VACCINATED WITH INACTIVATED JAPANESE ENCEPHALITIS VACCINE Thanyawee Puthanakit1, Linda Aurpibul1, Sutee Yoksan2, Thira Sirisanthana1 and Virat Sirisanthana3 1 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Center for Vaccine Development, Mahidol University, Bangkok, Thailand. 3 Division of Infectious Diseases, Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Among HIV-infected children who had immune recovery after received antiretroviral therapy (ART), good responses to revaccination with childhood vaccines have been observed. However, the rate of long-term persistence of antibody response remains unknown. The objective of this study is to determine whether HIV-infected children still have protective antibody against Japanese encephalitis virus (JE) 3 years after receiving revaccination with two doses of inactivated JE vaccine. Plasma JE neutralizing antibody titer was determined by a plaque reduction neutralization assay. An antibody titer of more than 1:10 was defined as being protective. Fifty HIV-infected children with a mean age of 10.3 years (SD 2.2) and mean CD4 percentage of 25 (SD 5) were revaccinated with two doses of inactivated JE vaccine. Forty-three children had been followed-up for 3 years. The JE neutralizing antibody at 1 month and 3 years after revaccination were detected among 38 (88%) and 35 (81%) children, respectively. The geometric means titer significantly dropped from of 306 (min 13-max 163,617) to 106 (min 11-max 4645). This data show that the majority of HIV-infected children had persistent antibody 3 years after revaccination. JE revaccination in HIV-infected children with immune recovery after ART should be carried out in endemic areas. © 2010 Elsevier Ltd. 12 Published in Vaccine. 2010 Aug 16;28(36):5900-2. A CHEWABLE PEDIATRIC FIXED-DOSE COMBINATION TABLET OF STAVUDINE, LAMIVUDINE, AND NEVIRAPINE PHARMACOKINETICS AND SAFETY COMPARED WITH THE INDIVIDUAL LIQUID FORMULATIONS IN HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CHILDREN IN THAILAND Nirun Vanprapar1, Tim R. Cressey2,3, Kulkanya Chokephaibulkit1, Petronella Muresan3, Nottasorn Plipat4, Virat Sirisanthana5, Wasana Prasitsuebsai1, Suchat Hongsiriwan6, Tawee Chotpitayasunondh7, Achara Eksaengsri8, Mari Pat Toye9, Mary Elizabeth Smith10, Kenneth McIntosh11, Edmund Capparelli12 and Ram Yogev13 1 Department of Pediatrics, Mahidol University, Siriraj Hospital, 2 Prannok Rd, Bangkok-noi, Bangkok 10700, Thailand. 2 Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Harvard School of Public Health, Boston, MA, United States. 4 School of Public Health, University of Michigan, Ann Arbor, MI, United States. 5 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. 6 Department of Pediatrics, Chonburi Regional Hospital, Chonburi, Thailand. 7 Queen Sirikit National Institute of Child Health (QSNICH), Bangkok, Thailand. 8 Government Pharmaceutical Organization (GPO), Bangkok, Thailand. 9 Department of Pediatrics, Baystate Medical Center, Springfield, MA, United States. 10 National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, United States. 11 Pediatric Pharmacology Research Unit, Children’s Hospital, Boston, MA, United States. 12 University of California, San Diego, CA, United States. 13 Chicago Children’s Memorial Hospital, Chicago, IL, United States. ABSTRACT Background: Pediatric fixed-dose combinations (FDCs) are needed to facilitate antiretroviral therapy in children. We evaluated the relative bioavailability, safety, and therapeutic adequacy of a novel chewable pediatric FDC tablet of stavudine (7 mg), lamivudine (30 mg), and nevirapine (50 mg), referred to as GPO-VIR S7, and compared it with the individual original brand-name liquid formulations in human immunodeficiency virus-infected Thai children. Methods: The International Maternal Pediatric Adolescent AIDS Clinical Trials group (IMPAACT) P1056 study was a phase I/II, 2-arm, randomized, open-label, multidose pharmacokinetic cross-over study. Children ≥6 to ≤30 kg receiving nevirapine-based HAART for at least 4 weeks were randomized to receive GPO-VIR S7 chewable tablets or the equivalent liquid formulations. Children were stratified by weight and dosing 13 was weight-based. Intensive 12-hour blood sampling was performed on day 28, and subjects then crossed-over to the alternate formulation at equal doses with identical 12-hour sampling on day 56. Pharmacokinetic indices were determined by noncompartmental analysis. Results: Thirty-four children completed the study. While taking Government Pharmaceutical Organization (GPO)-VIR S7 the geometric mean (90% CI) area under the curve was 1.54 µg•hr/mL (1.42-1.67) for stavudine, 6.39 (5.82-7.00) for lamivudine, and 74.06 (65.62-83.60) for nevirapine. Nevirapine drug exposure for GPO-VIR S7 was therapeutically adequate. Geometric mean area under the curve ratios (90% CI) of GPO-VIR S7/liquid formulation for stavudine, lamivudine, and nevirapine were 0.97 (0.92-1.02), 1.41 (1.30-1.53), and 1.08 (1.041.13), respectively. No serious drug-related toxicity was reported. Conclusions: The chewable FDC was safe and provided therapeutically adequate plasma drug exposures in human immunodeficiency virus-infected children. Substituting the FDC for liquid formulations can simplify antiretroviral therapy. 14 Published in Pediatr Infect Dis J. 2010 Oct;29(10):940-4. A RETROSPECTIVE STUDY OF OUTCOME IN PEDIATRIC LUPUS NEPHRITIS AFTER THE MAINTENANCE THERAPY BY AZATHIOPRINE OR ECMPS Wattana Chartapisak and Sauwalak Opastirakul Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Objectives: To report the outcome of lupus nephritis patients after the maintenance phase of oral immunosuppressive. Methods: As this is a retrospective study, we recruited the children who had lupus nephritis and had been treated by IVCY followed by Azathioprine or ECMPS between 2006 to 2010. The data includes age, sex, class of lupus nephritis, time to relapse and the complications. For the children who had relapsed, the time to relapse after the maintenance therapy was analyzed by the log rank test. Results: 35 children (F:M = 29 : 6) were recruited. The histopathology included2 children in class II (with crescent), 5 in class III, 22 in class IV and 4 in class V. The everage age is 10.8 yrs (5-14 yrs). 29 children received Azathioprine and 6 received ECMPS. There is none in ECMPS group who has relapsed before 2 years whereas 24% (7 cases) in Azathioprine group has relapsed while undertaking the Azathioprine. The over all relapse rate in Azathioprine group is 34.5% and 16% in ECMPS which is not statistically signifi cant (P= 0.08). The most frequent complications in Azathioprine group were neutropenia and hepatitis. There were fewer complications in the ECMPS group. Conclusions: Both Azathioprine and ECMPS were the maintenance therapy for Lupus nephritis. The patients in Azathioprine group tends to relapse more frequently while the patients not having ceased the treatment whereas ECMPS group has less relapse and still in the remission while the patient was continued the treatment.The RCT needs to prove the effi cacy of ECMPS for maintenance therapy in pediatric lupus nephritis. Published in Pediatr Nephrol (2010) 25:1779-2004. 15 AIR POLLUTION-RELATED PEAK EXPIRATORY FLOW RATES AMONG ASTHMATIC CHILDREN IN CHIANG MAI, THAILAND Phongtape Wiwatanadate1 and Muthita Trakultivakorn2 1 Department of Community Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT The severity of air pollution in northern Thailand has long been recognized; in spite of that there have been no epidemiological studies regarding the associations between the air pollution and health effects in the area. The authors followed a cohort of 31 asthmatic children (4-11 years of age) residing in Muang district, Chiang Mai, Thailand, from 29 August 2005 to 30 June 2006, for 306 days. The daily air pollutants, including particulate matter with aerodynamic diameter < 2.5 microm, particulate matter with aerodynamic diameter < 10 microm, carbon monoxide, ozone (O(3)), nitrogen dioxide, and sulfur dioxide (SO(2)), and the meteorological parameters, including pressure, temperature, relative humidity, rain quantity, and sunshine duration, were recorded. The peak expiratory flow rates (PEFRs) were fitted with pollutants and meteorological covariates using general linear mixed models to account for random effects and autocorrelation. The authors found that there were inverse associations of SO(2) and evening PEFR, with a coefficient of -2.12 (95% confidence interval (CI) = -3.22 to -0.28); of SO(2) and daily percent deviation of PEFR, with a coefficient of -0.73 (95% CI = -1.33 to -0.12); and of O(3) combining with SO(2) and daily average PEFR, with a coefficient of -0.16 (95% CI = -0.31 to -0.00) and -1.60 (95% CI = -3.10 to -0.11), respectively. The associations of O(3) and SO(2) with PEFR were found even when SO(2) concentrations never exceeded the standard level. 16 Published in Inhal Toxicol. 2010 Mar;22(4):301-8. AMBIENT PARTICULATE POLLUTION AND THE WORLD-WIDE PREVALENCE OF ASTHMA, RHINOCONJUNCTIVITIS AND ECZEMA IN CHILDREN: PHASE ONE OF THE INTERNATIONAL STUDY OF ASTHMA AND ALLERGIES IN CHILDHOOD (ISAAC) H Ross Anderson1, Ruth Ruggles1, Kiran D Pandey2, Venediktos Kapetanakis1, Bert Brunekreef3,4, Christopher K W Lai5, David P Strachan1, Stephan K Weiland6, and the ISAAC Phase One Study Group 1 Division of Community Health Sciences, MRC-HPA Centre for Environment and Health, St. George’s, University of London, Cranmer Terrace, London SW17 0RE, United Kingdom. 2 Environment Department, World Bank, Washington, DC, United States. 3 Institute for Risk Assessment Sciences, Utrecht University, Utrecht, Netherlands. 4 Julius Center for Health Sciences and Primary Care, University Medical Center, Utrecht, Netherlands. 5 Department of Medicine and Therapeutics, Chinese University of Hong Kong, Hong Kong, Hong Kong. ABSTRACT Objectives: To investigate the effect of ambient particulate matter on variation in childhood prevalence of asthma, rhinoconjunctivitis and eczema. Methods: Prevalences of asthma, rhinoconjunctivitis and eczema obtained in Phase One of the International Study of Asthma and Allergies in Childhood (ISAAC) were matched with city-level estimates of residential PM(10) obtained from a World Bank model. Associations were investigated using binomial regression adjusting for GNP per capita and for clustering within country. For countries with more than one centre, a two stage meta-analysis was carried out. The results were compared with a meta-analysis of published multi-centre studies. Results: Annual concentrations of PM(10) at city level were obtained for 105 ISAAC centres in 51 countries. After controlling for GNP per capita, there was a weak negative association between PM(10) and various outcomes. For severe wheeze in 13-14-year-olds, the OR for a 10 microg/m(3) increase in PM(10) was 0.92 (95% CI 0.84 to 1.00). In 24 countries with more than one centre, most summary estimates for within-country associations were weakly positive. For severe wheeze in 13-14-year-olds, the summary OR for a 10 microg/m(3) increase in PM(10) was 1.01 (0.92 to 1.10). This result was close to a summary OR of 0.99 (0.91 to 1.06) obtained from published 17 multi-centre studies. Conclusions: Modelled estimates of particulate matter at city level are imprecise and incomplete estimates of personal exposure to ambient air pollutants. Nevertheless, our results together with those of previous multi-centre studies, suggest that urban background PM(10) has little or no association with the prevalence of childhood asthma, rhinoconjunctivitis or eczema either within or between countries. 18 Published in Occup Environ Med. 2010 May;67(5):293-300. AN ENZYME-LINKED IMMUNOSORBENT ASSAY AS SCREENING TOOL FOR HUMAN INTESTINAL CAPILLARIASIS Pewpan M Intapan1, Wanchai Maleewong1, Watana Sukeepaisarnjaroen2 and Nimit Morakote3 1 Department of Parasitology Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. 2 Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. 3 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Human intestinal capillariasis caused by Capillaria philippinensis is characterized by chronic diarrhea which may lead to death if left untreated. The mortality is highest among patients who are negative by conventional stool examination. Therefore this study explored the application of an enzyme-linked immunosorbent assay (ELISA) as a screening test for human intestinal capillariasis. The ELISA was developed using Trichinella spiralis soluble antigen for the detection of antibodies against C. philippinensis. A cut-off level at the upper 99% limit of the absorbance values of the healthy controls was established for positivity. All intestinal capillariasis sera showed positive ELISA, demonstrating 100% sensitivity, while all healthy control sera gave absorbance values below the cut-off level, resulting in 100% specificity. The ELISA was also positive with 75% of trichinellosis, 13.9% of strongyloidiasis, 9.1% of trichuriasis, and 4.2% of opisthorchiasis sera. The ELISA and immunoblot were in agreement in 91.1% of the sera tested. It was suggested that the here-presented ELISA is capable to detect intestinal capillariasis cases in endemic areas whose coproscopy is negative for worm eggs, larvae or adults. Published in Southeast Asian Journal of Tropical Medicine and Public Health (2010) 41(2): 298-305. 19 ANALYSIS OF FEMALE SALIVARY GLAND PROTEINS OF THE ANOPHELES BARBIROSTRIS COMPLEX (DIPTERA: CULICIDAE) IN THAILAND Narissara Jariyapan1, Visut Baimai2, Yong Poovorawan3, Sittiruk Roytrakul4, Atiporn Saeung1, Sorawat Thongsahuan1, Sittiporn Suwannamit1, Yasushi Otsuka5 and Wej Choochote1 1 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Biology and Center for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand. 3 Department of Pediatric, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. 4 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand. 5 Department of Infectious Disease Control, Faculty of Medicine, Oita University, Hasama, Oita 879-5593, Japan. ABSTRACT Electrophoretic protein profiles of female salivary glands of five sibling species within the Anopheles barbirostris complex, namely A. barbirostris species A1 (Forms A, B, and D), A2, A3, and A4 and Anopheles campestris-like (Forms B and E), were analyzed. At least eight major and several minor protein bands were detected in the glands of each species, of which each morphological region contained different major proteins. The protein profiles distinguished the five sibling species. The variability in major proteins among species was observed in the 40-48, 32-37, and 10-18 kDa ranges. No difference in protein profiles was found in different cytogenetic forms. Polymorphism of the protein profiles within species was only noted in species A4. The lowest major protein (marker) band of each species showed remarkably different relative mobility on SDS-polyacrylamide gels. NanoLC-MS analysis revealed that the marker protein of some species matched with a protein involving in blood feeding, gSG6, of Anopheles gambiae and Anopheles freeborni. These results might be useful for construction of an additional tool to distinguish the five sibling species and lead to further study on the evolution of blood feeding and pathogen transmission. 20 Published in Parasitology Research (2010) 107:509-516. Anesthesia-related complications of caesarean delivery in Thailand: 16,697 cases from the Thai Anaesthesia Incidents Study Chau-in W.1, Hintong T.2, Rodanant O.3, Lekprasert V.4, Punjasawadwong Y.2, Charuluxananan S.3 and Tanudsintum S.5 1 Department of Anesthesiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. 2 Department of Anesthesiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Anesthesiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 4 Department of Anesthesiology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. 5 Department of Anesthesiology, Faculty of Medicine, Phramongkutklao College of Medicine, Bangkok, Thailand. ABSTRACT Background: Maternal complications related to anesthesia are low in comparison with the results from obstetric factors in developing countries. The purposes of the present study were to determine the incidence of maternal mortality related to anesthesia, to analyze the causes and to suggest measures to improve anesthetic safety for the parturients. Material and Method: The present study was part of a multi-center study conducted by the Royal College of Anesthesiologists of Thailand aimed at surveillance of anesthesia-related complications in Thailand. The authors conducted a prospective survey of hospital records from all of the cases in and outside the operating room receiving general anesthesia in 18 centers between March 1, 2003 and February 28, 2004. All the forms were checked and verified by three-peer review then included in the analysis, using descriptive statistics. Results: Sixteen thousand six hundred ninety seven cases were included. The incidence of anesthetic complication in parturients was 35.9: 10,000 (95% CI 27.4, 46.1). Incidence ofthe four most common anesthetic related adverse events in caesarean section were desaturation 13.8 (95% CI 8.7, 20.7), cardiac arrest 10.2 (95% CI 5.9, 16.3), awareness 6.6 (95% CI 3.3, 11.8), and death related anesthesia 4.8 (95% CI 2.17, 9.4). Of these, seven (17.5%) had preeclampsia/eclampsia and 46 (76.7%) presented for emergency caesarean delivery. General anesthesia was used 21 in 41 patients (68.4%) and spinal in eighteen (30%). There were eight maternal deaths including five with general anesthesia, giving a case fatality rate of 0.1% of general anesthetics or 0.3% of caesarean deliveries. Conclusion: The authors found that inexperience, inadequate knowledge, inadequate care, and patient conditions were the major contributory factors. Most of them were preventable and correctable. Additional training and quality assurance can improve and prevent these serious adverse events. 22 Published in J Med Assoc Thai. 2010 Nov;93(11):1274-83. Published in PubMed PMID: 21114206. ANTIRETROVIRAL THERAPY OUTCOMES OF HIV-INFECTED CHILDREN IN THE TREAT ASIA PEDIATRIC HIV OBSERVATIONAL DATABASE Rawiwan Hansudewechakul1, Virat Sirisanthana2, Nia Kurniati3, Thanyawee Puthanakit4, Pagakrong Lumbiganon5, Vonthanak Saphonn6, Nik Khairulddin Nik Yusoff7, Nagalingeswaran Kumarasamy8, Siew Moy Fong9, Revathy Nallusamy10, Preeyaporn Srasuebkul11, Matthew Law11, Annette H Sohn13, Kulkanya Chokephaibulkit14; TREAT Asia Pediatric HIV Observational Database 1 Department of Pediatrics, Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand. Department of Pediatrics, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Pediatrics, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia. 4 HIV-NAT, Thai Red Cross AIDS Research Centre, Bangkok, Thailand. 5 Department of Pediatrics, Khon Kaen University, Khon Kaen, Thailand. 6 National Centre for HIV/AIDS, Dermatology and STDs, Phnom Penh, Cambodia. 7 Department of Pediatrics, Hospital Raja Perempuan Zainab II, Kota Bharu, Malaysia. 8 YR Gaitonde Centre for AIDS Research and Education, Chennai, India. 9 Department of Pediatrics, Hospital Likas, Kota Kinabalu, Malaysia. 10 Department of Pediatrics, Penang Hospital, Penang, Malaysia. 11 National Centre in HIV Epidemiology and Clinical Research, University of New South Wales, Sydney, Australia. 12 TREAT Asia/amfAR-The Foundation for AIDS Research, Bangkok, Thailand. 13 Department of Pediatrics, Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkok-noi, Bangkok 10700, Thailand. 2 ABSTRACT Introduction: We report responses to combination antiretroviral therapy (cART) in the Therapeutics Research, Education, and AIDS Training in Asia Pediatric HIV Observational Database. Methods: Children included were those who had received cART (ie, ≥3 antiretrovirals) at <18 years. The analysis was intention-to-treat by the first cART regimen. Median values are provided with interquartile ranges; hazard ratios (HRs) with 95% confidence intervals. Results: Of the 1655 children included, 50.4% were male, with a median age at cART of 7.0 (3.9-9.8) years and CD4 of 8% (2.0%-15%); 92.5% were started on an NNRTI; median duration of follow-up was 2.9 (1.4-4.6) years. Loss-to-follow-up and death rates were 4.2 (3.7-4.8) and 2.1 (1.7-2.5) per 100 person-years, respectively. At 36 months, median CD4 was 26% (21%-31%); 81% of those with viral load (n = 302) were <400 copies per milliliter. Children who reached CD4 ≥25% within 5 years were more likely to be females (HR: 1.4; 1.2-1.7), start before 18 months old (HR: 3.8; 2.4-6.2), lack a history of monotherapy/dual therapy (HR: 1.7; 1.4-2.5), and have a higher baseline CD4 (per 10% increase: HR: 2; 1.9-2.2). Conclusions: These data underscore the need for early diagnosis and cART initiation to preserve immune function. Published in J Acquir Immune Defic Syndr. 2010 Dec 1;55(4):503-9. 23 Bone resorption acceleration and calcium reabsorption impairment in a Thai population with high cadmium exposure Kowit Nambunmee1, Ryumond Honda2, Muneko Nishijo2, Witaya Swaddiwudhipong3, Hideaki Nakagawa2 and Werawan Ruangyuttikarn4 1 Doctor of Philosophy Program in Biomedical Sciences, Faculty of Associated Medical Sciences, Chiang Mai University 50200, Thailand. 2 Department of Public Health, Kanazawa Medical University, Ishikawa, Japan. 3 Department of Community and Social Medicine, Mae Sot General Hospital, Tak Province, Thailand. 4 Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University 50200, Thailand. ABSTRACT Some residents of the Mae Sot district in Thailand have suffered longterm exposure to elevated dietary levels of cadmium. To test the hypothesis that chronic dietary cadmium exposure can cause imbalance in calcium dynamics and accelerate bone resorption, a group of these residents (156 men and 256 women aged >/= 50) were selected on the basis of previous records of elevated urinary cadmium and tested for urinary and blood cadmium, bone formation and resorption markers, and the renal tubular dysfunction markers. Both genders had high levels of blood and urinary cadmium and high urinary levels of the markers for renal dysfunction and bone resorption in a dose-response relationship to urinary cadmium. The excretion of bone resorption markers was positively correlated to the ratio of excreted calcium and urinary cadmium. The results of a multivariate regression analysis indicated that bone resorption was accelerated by impaired calcium reabsorption in renal tubules. 24 Published in January 2010, Vol.20, No.1 , Pages 7-13 (doi:10.3109/15376510903452941). Cadmium induced renal dysfunction among residents of rice farming area downstream from a zinc-mineralized belt in Thailand Ryumon Honda1, Witaya Swaddiwudhipong2, Muneko Nishijo3, Pranee Mahasakpanb2, Wimonrat Teeyakasemd4, Werawan Ruangyuttikarnd4, Soisungwan Sataruge5, Chantana Padungtodf6 and Hideaki Nakagawac3 1 Department of Social and Environmental Health, Kanazawa Medical University, Uchinada, Ishikawa, Japan. 2 Mae Sot Hospital, Tak Province, Thailand. 3 Department of Public Health, Kanazawa Medical University, Uchinada, Ishikawa, 1-1 Daigaku, Uchinada, Ishikawa 920-0293, Japan. 4 Division of Toxicology, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. 5 Center for Kidney Disease Research, University of Queensland School of Medicine, Princess Alexandra Hospital, Wolloonggabba, Brisbane, Australia. 6 Bureau of Occupational and Environmental Disease, Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand. ABSTRACT High levels of cadmium (Cd) in paddy soil and rice, and a concurrent increase in urinary Cd of inhabitants in the Mae Sot District, Tak Province, Thailand first emerged in 2003. Risk factors such as occupation, sources of staple food and drinking water as well as biomarkers of adverse renal effects of the increased body burden of Cd were investigated in 795 residents, 30 years or older, whose urinary Cd levels were between 0.02 and 106mug/g creatinine. Farmers who consumed their own rice and residents who sourced drinking water from wells and/or the river demonstrated increased urinary Cd. Age-adjusted mean urinary cadmium for male and female subjects who consumed well water was 5.7mug/g vs. 6.1mug/g creatinine while the corresponding value for male and female subjects who did not consume well water was 3.7mug/g vs. 4.8mug/g creatinine, respectively. Increased urinary levels of beta(2)-microglobulin and N-acetyl-beta-d-glucosaminidase were observed with the increase in urinary Cd, indicating tubular dysfunction and renal damage associated with increase in Cd body burden. Consumption of well water and rice grown in the contaminated area increase the body burden of Cd. Cessation of exposure is essential to prevent and possibly recover from Cd toxicity, especially among the residents whose kidney damage and malfunction may be reversible.2010 Elsevier Ireland Ltd. All rights reserved. Published by selected papers from An International Conference on Cadmium and Human Health: Technologies for Environmental Restoration and Rehabilitation (doi:10.1016/j. toxlet.2010.04.023) in Volume 198, Issue 1, 15 September 2010, Pages 26-32. 25 CELL RESERVOIRS OF THE EPSTEIN-BARR VIRUS IN BIOPSY-PROVEN LYMPHOCYTIC INTERSTITIAL PNEUMONITIS IN HIV-1 SUBTYPE E INFECTED CHILDREN IDENTIFICATION BY COMBINED IN SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY Lertlakana Bhoopat1, Somrak Rangkakulnuwat2, Risa Okonogi3, Komson Wannasai1 and Tanin Bhoopat3 1 Departments of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Departments of Pediatrics, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Departments of Forensic Medicine, DNA unit, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Lymphoid interstitial pneumonitis (LIP), a frequent pulmonary complication in human immune deficiency virus (HIV)-infected pediatric patients, is characterized histologically by marked infiltration of lymphoid cells. Several theories have been suggested that LIP may be caused by Epstein-Barr virus (EBV). To identify the reservoir of EBV and pathogenesis of lymphoid infiltrates in HIV subtype E infected pediatric LIP, we examined the distribution and expression of EBV in the inflammatory cell recruitment in surgical lung biopsy-proven LIP from 9 vertically HIV subtype E-infected pediatric patients. The dominant microscopic feature of LIP demonstrated widespread widening of alveolar septum by mononuclear inflammatory cell infiltrate mainly composed of mature lymphocytes and plasma cells surrounding airways and expanding to the lung interstitium. EBV-encoded RNA (EBER) in situ hybridization, performed from paraffin-embedded lung tissues, revealed positive intranuclear signals in all 9 LIP cases. Interestingly, combined immunohistochemical and in situ hybridization analyses in 6 out of 9 LIP cases revealed 30% to 50% of the Langerhans and related dendritic cells were infected with EBV, whereas <30% of the T and B cells were infected with EBV. These results suggested that a chronic antigenic stimulus of EBV played important roles in the pathogenesis of LIP in these patients. This supports the notion that Langerhans cells (LCs) are more readily infected with EBV, indicating that LCs are reservoirs for EBV in lungs of HIV subtype E-infected pediatric LIP. And possibly LCs may play an important role in the recruitment of inflammatory cell infiltrates, especially T cells into these tissues. In addition, HIV may provide a milieu or microenvironment for the evolution of LIP, which represent an immunologic response to EBV infection. Interactions between LCs and related dendritic cells together with T cells are important for effective HIV and EBV replications. 26 Published in Appl Immunohistochem Mol Morphol. 2010 May;18(3):212-8. CHARACTERISTICS OF LYMPHOCYTE SUBSETS IN HIV-INFECTED, LONG-TERM NONPROGRESSOR, AND HEALTHY ASIAN CHILDREN THROUGH 12 YEARS OF AGE Jintanat Ananworanich1,2,3, Tanakorn Apornpong1, Pope Kosalaraksa4, Tanyathip Jaimulwong1, Rawiwan Hansudewechakul5, Chitsanu Pancharoen3, Torsak Bunupuradah1, Mom Chandara6, Thanyawee Puthanakit1,3, Chaiwat Ngampiyasakul7, Jurai Wongsawat8, Suparat Kanjanavanit9, Wicharn Luesomboon10, Phennapha Klangsinsirikul11, Nicole Ngo-Giang-Huong12, Stephen J. Kerr1,13, Sasiwimol Ubolyam1, Tawan Mengthaisong1, Rebecca S. Gelman14,15, Kovit Pattanapanyasat16, Vonthanak Saphonn6,17, Kiat Ruxrungtham1,3, William T. Shearer18 and PREDICT Study Group 1 HIV Netherlands Australia Thailand Research Collaboration, Thai Red Cross AIDS Research Center, Bangkok, Thailand. 2 South East Asia Research Collaboration With Hawaii, Bangkok, Thailand. 3 Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 4 Khon Kaen University, Khon Kaen, Thailand. 5 Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand. 6 National Institute of Public Health, Phnom Penh, Cambodia. 7 Prapokklao Hospital, Chantaburi, Thailand. 8 Bamrasnaradura Infectious Diseases Institute, Nonthaburi, Thailand. 9 Nakornping Hospital, Chiang Mai, Thailand. 10 Queen Savang Vadhana Memorial Hospital, Chonburi, Thailand. 11 Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand. 12 IRD U174 Program for HIV Prevention and Treatment, Chiang Mai, Thailand. 13 National Center in HIV Epidemiology and Clinical Research, Sydney, Australia. 14 Harvard School of Public Health, Boston, MA, United States. 15 Dana-Farber Cancer Institute, Boston, MA, United States. 16 Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand. 17 National Center for HIV/AIDS, Dermatology and STDs, Phnom Penh, Cambodia. 18 Baylor College of Medicine, Houston, TX, United States. ABSTRACT Background: There are limited data on the immune profiles of HIV-positive children compared with healthy controls, and no such data for Asian children. Objectives: To immunophenotype HIV-positive Asian children, including long-term nonprogressors (LTNPs), compared with age-matched healthy controls. Methods: We used flow cytometry to analyze 13 lymphocyte and monocyte subsets from 222 untreated, HIV-positive children with 15% to 24% CD4+ T cells and no AIDS-related illnesses and 142 healthy children (controls). Data were compared among age categories. Profiles from LTNPs (n = 50), defined as children ≥8 years old with CD4+ T-cell counts ≥350 cells/mm3, were compared with data from age-matched non-LTNPs (n = 17) and controls (n = 53). Results: Compared with controls, HIV-positive children had lower values (cell count per mm3 and percent distribution) for TH cells and higher values for cytotoxic T cells, with reductions 27 in populations of naive TH and cytotoxic T cells, B cells, and natural killer (NK) cells. HIV-positive children had high values for activated TH and cytotoxic T cells. Compared with non-LTNPs, LTNPs had higher values of TH and cytotoxic T cells, naive and memory T-cell subsets, and B and NK cells. Surprisingly, counts of activated TH and cytotoxic T cells were also higher among LTNPs. LNTPs were more frequently male. Conclusion: Untreated, HIV-infected Asian children have immune profiles that differ from those of controls, characterized by low values for TH cells, naive T cells, B cells, and NK cells but high values for cytotoxic, activated TH, and cytotoxic T cells. The higher values for activated T cells observed in LTNPs require confirmation in longitudinal studies. 28 Published in J Allergy Clin Immunol. 2010 Dec;126(6):1294-301.e10. CHEMICAL COMPOSITION AND LARVICIDAL ACTIVITY OF EDIBLE PLANT-DERIVED ESSENTIAL OILS AGAINST THE PYRETHROIDSUSCEPTIBLE AND -RESISTANT STRAINS OF AEDES AEGYPTI (DIPTERA: CULICIDAE) Nataya Sutthanont, Wej Choochote, Benjawan Tuetun, Anuluck Junkum, Atchariya Jitpakdi, Udom Chaithong, Doungrat Riyong and Benjawan Pitasawat Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The chemical compositions and larvicidal potential against mosquito vectors of selected essential oils obtained from five edible plants were investigated in this study. Using a GC/MS, 24, 17, 20, 21, and 12 compounds were determined from essential oils of Citrus hystrix, Citrus reticulata, Zingiber zerumbet, Kaempferia galanga, and Syzygium aromaticum, respectively. The principal constituents found in peel oil of C. hystrix were β-pinene (22.54%) and d-limonene, (22.03%) followed by terpinene-4-ol (17.37%). Compounds in C. reticulata peel oil consisted mostly of d-limonene (62.39%) and γ-terpinene (14.06%). The oils obtained from Z. zerumbet rhizome had α-humulene (31.93%) and zerumbone (31.67%) as major components. The most abundant compounds in K. galanga rhizome oil were 2-propeonic acid (35.54%), pentadecane (26.08%), and ethyl-p-methoxycinnamate (25.96%). The main component of S. aromaticum bud oil was eugenol (77.37%), with minor amounts of trans-caryophyllene (13.66%). Assessment of larvicidal efficacy demonstrated that all essential oils were toxic against both pyrethroid-susceptible and resistant Ae. aegypti laboratory strains at LC50, LC95, and LC99 levels. In conclusion, we have documented the promising larvicidal potential of essential oils from edible herbs, which could be considered as a potentially alternative source for developing novel larvicides to be used in controlling vectors of mosquito-borne disease. Published in Journal of Vector Ecology (2010) 35(1): 106-115. 29 Comparison of efficacy and effectiveness between ULTRACET and tramadol/acetaminophen in acute postoperative pain after upper extremity surgery Passakorn Sawaddiruk, Sahattaya Paiboonworachat and Kittichai Janthawichai Department of Anesthesiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. ABSTRACT Objective: To compare the efficacy and effectiveness between ULTRACE, a new analgesic combination of tramadol/ acetaminophen, and tramadol/acetaminophen for acute postoperative pain after upper extremity surgery. Study Design: A double blind randomized controlled trial. Material and Method: One hundred and eighty patients who underwent upper extremity surgery under brachial plexus block were randomized to receive either ULTRACE (n = 87) or tramadol and acetaminophen (n = 93) immediately after surgery in the postanesthetic care unit (PACU). Total morphine requirement, pain score (VRS) and drug adverse effects were compared between those two groups using independent single t-test, Mann-Whitney U-test, Chi-square test and Fisher-exact test respectively. Results: Total morphine requirement was significantly lower in subjects who used ULTRACE when compared with the tramadol and acetaminophen group (0.51 and 0.69 mg in the first 6 hours after the operation, 0.0 and 0.13 mg in hours 6-12 after the operation). Moreover there were fewer side effects in this ULTRACE group too. Conclusion: ULTRACET has more efficacy and fewer side effects when compared with tramadol and acetaminophen in acute postoperative pain surgery. 30 Published in J Med Assoc Thai. 2010 Jul;93(7):812-7. CORD BLOOD SCREENING FOR ALPHA-THALASSEMIA AND HEMOGLOBIN VARIANTS BY ISOELECTRIC FOCUSING IN NORTHERN THAI NEONATES: CORRELATION WITH GENOTYPES AND HEMATOLOGIC PARAMETERS Pimlak Charoenkwan1, Rawee Taweephol1, Supatra Sirichotiyakul2, Watcharee Tantiprabha3, Rattika Sae-Tung1, Sudjai Suanta1, Pimonrat Sakdasirisathaporn1 and Torpong Sanguansermsri1 1 Division of Hematology, Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Division of Neonatology, Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT We describe the screening of newborns for thalassemia and Hb variants by using isoelectric focusing (IEF) in a population from northern Thailand where hemoglobinopathies are highly prevalent. The report focuses on findings of alphathalassemia, Hb E, and other hemoglobin variants, and their correlation with genotypes and hematologic parameters. Two-hundred and seven out of 566 newborns (36.6%) had thalassemia genes or Hb variants. Seventeen different genotypes were found. Nine cases (1.6%) of Hb H disease (five deletional Hb H diseases, two Hb H/Constant Spring diseases, one deletional Hb H disease/Hb E, carrier and one Hb H/Constant Spring disease/Hb E carrier) and one Hb E-beta-thalassemia were identified. IEF could clearly distinguish Hb H diseases and carriers of two alphaglobin gene defects from normal individuals according to the presence of Hb Bart’s and its percentage. For carriers of a single alpha-globin gene defect, Hb Bart’s was either absent or present in a small amount and was therefore not reliable for screening. The presence of an additional band at the Hb A(2) position in the newborns signified an Hb E carrier. One case of an absent Hb A and a presence of Hb E was identified as Hb E-beta-thalassemia. Two Hb Q-Thailand carriers were seen with two additional Hb fractions, presumably combinations of gamma-globin and beta-globin with the alpha-globin variant. Newborns with Hb H disease had lower Hb, MCV, and MCH levels than normal. MCV and MCH were also useful for differentiation of carriers of two alpha-globin gene defects, but not for carriers of Hb E or single alpha-globin gene defect. IEF was a reliable method for neonatal cord blood screening for alpha-thalassemia and Hb variants. Published in Blood Cells Mol Dis. 2010 Jun 15;45(1):53-7. 31 DEFERASIROX IN IRON-OVERLOADED PATIENTS WITH TRANSFUSION-DEPENDENT MYELODYSPLASTIC SYNDROMES: RESULTS FROM THE LARGE 1-YEAR EPIC STUDY Norbert Gattermann1, Carlo Finelli2, Matteo Della Porta3, Pierre Fenaux4, Arnold Ganser5, Agnes Guerci-Bresler6, Mathias Schmid7, Kerry Taylor8, Dominique Vassilieff9, Dany Habr10, Gabor Domokos11, Bernard Roubert11, Christian Rose12 and on behalf of the EPIC study investigators 1 Heinrich-Heine-University, Düsseldorf, Germany. Policlinico S. Orsola-Malpighi, Bologna, Italy. 3 University of Pavia Medical School, IRCCS Policlinico S. Matteo, Pavia, Italy 4 Hôpital Avicenne, Assistance Publique-Hôpitaux de Paris, Paris 13 University, Bobigny, France. 5 Medizinische Hochschule Hannover, Hannover, Germany. 6 CHU Brabois, Vandoeuvre Cedex, France. 7 University Hospital, Ulm, Germany. 8 Mater Hospital, Brisbane, Australia. 9 Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris 5 University, Paris, France. 10 Novartis Pharmaceuticals, East Hanover, NJ, USA. 11 Novartis Pharma AG, Basel, Switzerland. 12 Hôpital Saint-Vincent de Paul, Lille, France. 2 ABSTRACT The prospective 1-year EPIC study enrolled 341 patients with myelodysplastic syndromes (MDS); although baseline iron burden was >2500ng/mL, approximately 50% were chelation-naïve. Overall median serum ferritin decreased significantly at 1 year (p=0.002). Decreases occurred irrespective of whether patients were chelation-naïve or previously chelated; changes were dependent on dose adjustments and ongoing iron intake. Sustained reductions in labile plasma iron were observed. Discontinuation rate (48.7%) and adverse event profile were consistent with previously reported deferasirox data in MDS. Alanine aminotransferase levels decreased significantly; change correlated significantly with reduction in serum ferritin (p<0.0001). This large dataset prospectively confirms the efficacy and well characterizes the safety profile of deferasirox in MDS. 32 Published in Leuk Res. 2010 Sep;34(9):1143-50. Diagnosing delirium in elderly Thai patients: utilization of the CAM algorithm Nahathai Wongpakaran1, Tinakon Wongpakaran1, Putipong Bookamana2, Manee Pinyopornpanish1, Benchalak Maneeton1, Peerasak lerttrakarnnon3, Kasem Uttawichai3 and Surin Jiraniramai3 1 Department of Psychiatry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Statistics, Faculty of Sciences, Chiang Mai University, Chiang Mai 50200, Thailand 3 Department of Family Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT Background: Delirium is a common illness among elderly hospitalized patients. However, under-recognition of the condition by non-psychiatrically trained personnel is prevalent. This study investigated the performance of family physicians when detecting delirium in elderly hospitalized Thai patients using the Thai version of the Confusion Assessment Method (CAM) algorithm. Methods: A Thai version of the CAM algorithm was developed, and three experienced Thai family physicians were trained in its use. The diagnosis of delirium was also carried out by four fully qualified psychiatrists using DSM-IV TR criteria, which can be considered the gold standard. Sixty-six elderly patients were assessed with MMSE Thai 2002, in order to evaluate whether they had dementia upon admission. Within three days of admission, each patient was interviewed separately by a psychiatrist using DSM-IV TR, and a family physician using the Thai version of the CAM algorithm, with both sets of interviewers diagnosing for delirium. Results: The CAM algorithm tool, as used by family physicians, demonstrated a sensitivity of 91.9% and a specificity of 100.0%, with a PPV of 100.0% and an NPV of 90.6%. Interrater agreement between the family physicians and the psychiatrists was good (Cohen’s Kappa = 0.91, p < 0.0001). The mean of the time the family physicians spent using CAM algorithm was significantly briefer than that of the psychiatrists using DSM-IV TR. Conclusions: Family physicians performed well when diagnosing delirium in elderly hospitalized Thai patients using the Thai version of the CAM algorithm, showing that this measurement tool is suitable for use by non-psychiatrically trained personnel, being short, quick, and easy to administer. However, proper training on use of the algorithm is required. Published in BMC Family Practice 2011, 12:65doi:10.1186/1471-2296-12-65. 33 DIFFERENTIATION BETWEEN LUCILIA CUPRINA AND HEMIPYRELLIA LIGURRIENS (DIPTERA: CALLIPHORIDAE) LARVAE FOR USE IN FORENSIC ENTOMOLOGY APPLICATIONS Kom Sukontason1, Pongruk Sribanditmongkol2, Ratchadawan Ngoen-klan1, Tunwadee Klong-klaew1, Kittikhun Moophayak1 and Kabkaew L. Sukontason1 1 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand 2 Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT Lucilia cuprina and Hemipyrellia ligurriens are forensically important blow flies in many countries. The immature stage, in particular the third instar, of both species are generally similar in morphological appearance. In this study, the use of three main characteristics, the posterior spiracle, the dorsal spines between the first and second thoracic segments, and the cephalopharyngeal skeleton, allows for identification of both fly species, with the number of papillae on anterior spiracle being a supportive characteristic in some cases. Comparison of these characteristics is illustrated in detail. 34 Published in Parasitology Research (2010) 106:641-646. EFFECT OF LANNA MUSIC LISTENING AND MASSAGE ON EMOTIONAL AND PHYSIOLOGICAL CHANGE: PRELIMNARY DATA FROM THAI VOLUNTEER GROUP Peerasak Lerttrakarnnon1, Boosong Kasempitakpong1, Winthana Kusirisin1, Anuchart Matanasarawoot1, Polkae Vacharachaisurapol2 and Anchaya Dutjanuthat2 1 Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. Chiang Mai Provincial Public Health Office, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT The study measured the effects of the new traditional Lanna-style music (called “Lanna keetabambud”) on the emotions and physiological changes to volunteers. This was a non-randomized cross-over clinical trial study for 60 volunteers between May-October 2010. Post-relaxation score of Thai volunteer group was highest in the music listening during massage test. Published in Proceedings of the 13th WFMT World Congress of Music Therapy, July 5-9, 2011 in Seoul, Korea. 35 FORENSICALLY IMPORTANT FLESH FLY SPECIES IN THAILAND: MORPHOLOGY AND DEVELOPMENTAL RATE Kom Sukontason1, Nophawan Bunchu2, Tarinee Chaiwong3, Kittikhun Moophayak1 and Kabkaew L. Sukontason1 1 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand 2 Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang, Phitsanulok 65000, Thailand 3 College of Medicine and Public Health, Ubon Ratchathani University, 85 Sathollmark Rd., Warinchamrap, Ubon Ratchathani 34190, Thailand ABSTRACT Forensically important flesh fly species in Thailand have been investigated for their larval morphology and developmental rate to increase the forensic entomology database in Thailand and nearby countries. Sarcophaga (Liosarcophaga) dux Thomson, Sarcophaga (Liopygia) ruficornis (Fabricius), and Sarcophaga (Boettcherisca) peregrina (Robineau-Desvoidy) are the species of greatest forensic importance. The third instars of these species are similar in morphological appearance, making it difficult or impossible to differentiate between them based on external morphological characteristics. This study compares these important characteristics and reveals that the most distinctive is the posterior spiracle, followed by the number of papillae on the anterior spiracle. For confirmation of these species, the unique characteristics of adult males are also shown for accurate identification. Both the third instar and adult male characteristics are keys to identifying these species. In addition, the developmental rate of S. dux was examined in northern Thailand during 2002-2003 under natural ambient temperature (≈24-28°C) and a natural light/dark photoperiod (≈12:12 h), indicating relatively rapid larval development in summer (March-June), pre-pupae (stop moving) initiated at 72 h. In the rainy season (July-October), pre-pupae initiated at 72 or 96 h, whereas pre-pupae initiated at 96 h in winter. 36 Published in Parasitology Research (2010) 106:1055-1064. Fractures of the hyoid bone and thyroid cartilage in suicidal hanging Nantana Charoonnate2, Pitoon Narongchai1 and Somsak Wongwaiwate1 1 Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University 50200, Thailand. 2 Division of Emergency Medicine Forensic - Suratthani hospital, Suratthani Province 84000, Thailand. ABTRACT Objective: To analyze the frequency of hyoid bone and thyroid cartilage fractures caused by suicidal hanging in Thai people and compare the different methods of visualizing the those fractures. Results could be used for forensic purposes. Material and Method: A prospective study of hyoid bone and thyroid cartilage fractures in Thai people who died from suicidal hanging between November 2008 and August 2009. The authors confirmed suicides, based on history, crime scene investigation, autopsy reports, and other police information, visual and palpatory examination, and stereomicroscopy with or without 1% Toluidine. Neck dissection was performed following the protocol of Prinsloo and Gordon. Results and data analysis were performed via SPSS version 16. Results: Twenty male cases of suicidal hanging were reviewed. Fractures of the hyoid bone and/or thyroid cartilage were found in five cases (25%). Two cases ofthe hyoid bone fracture, two cases of thyroid cartilage fracture, and one case was both bone fractures. Mean age of all cases, non-fracture, and fracture cases were 42.40, 35.93, and 61.80 years, respectively The knot was at the back of the neck in most cases (12 cases, 60%) and at the left, front, and right in four, two, and two cases, respectively. However there was no relation between location ofthe knot at the neck and fractures of hyoid bone and thyroid cartilage (p=1). Incomplete and complete hanging were found in 11 and nine cases. Five cases with fractures of the hyoid bone and/or thyroid cartilage were incomplete hanging. The visual and palpatory examination did not detect the fracture of hyoid bone in all 20 cases but stereomicroscopy and stereomicroscopy with Toluidine blue stain detected fracture in three cases. For detecting thyroid cartilage fractures, all three methods had the same result. Conclusion: Fractures of the hyoid bone and thyroid cartilage in 25% of Thais who died of suicidal hanging were related with older ages and incomplete hanging but not related with location of the knot. The stereomicroscopic method is fast and effective in detecting fractures of the hyoid bone and thyroid cartilage. Published in J Med Assoc Thai 2010; 93 (10): 1211-6Full text. e-Journal:http://www.mat. or.th/journal. 37 FULMINANT ENTEROVIRUS 71 INFECTION: CASE REPORT Krit Makonkawkeyoon1, Tavitiya Sudjaritruk2, Virat Sirisanthana2 and Suchaya Silvilairat1 1 Divisions of Pediatric Cardiology, Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Infectious Diseases, Department of Pediatrics, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A previously healthy 3-year-old boy presented with high-grade fever, dyspnoea, alteration of consciousness, tachycardia and shock. A few erythematous macules and papules were seen on his palms and soles. Echocardiogram showed poor left ventricular contraction. Cardiac enzymes and pro-B-type natriuretic peptide were elevated. Milrinone, low-dose dopamine and intravenous immunoglobulin were administered. The patient recovered after 5 days without cardiac or neurological sequelae. The serological results showed a four-fold rise of enterovirus 71. In children with severe EV71 infection, early recognition of cardiopulmonary involvement and aggressive treatment are crucial to successful management. 38 Published in Annals of Tropical Paediatrics (2010) 30, 245-248. HIV-1 DRUG RESISTANCE MUTATIONS IN CHILDREN AFTER FAILURE OF FIRST-LINE NONNUCLEOSIDE REVERSE TRANSCRIPTASE INHIBITORBASED ANTIRETROVIRAL THERAPY HIV-1 DRUG RESISTANCE MUTATIONS IN CHILDREN AFTER FAILURE OF FIRST-LINE NONNUCLEOSIDE REVERSE TRANSCRIPTASE INHIBITOR-BASED ANTIRETROVIRAL THERAPY Thanyawee Puthanakit1,2,3, Gonzague Jourdain4, Suchat Hongsiriwon5, Piyarat Suntarattiwong6, Kulkanya Chokephaibulkit7, Virat Sirisanthana2,8, Pusadee Kosalaraksa9, Wittaya Petdachai10, Rawiwan Hansudewechakul11, Umaporn Siangphoe1, Tulathip Suwanlerk1 and Jintanat Ananworanich1,12,13, HIV-NAT 086Study Team 1 The HIV Netherlands Australia, Thailand Research Collaboration (HIV-NAT), Bangkok, Thailand. Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 3 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Program for HIV Prevention and Treatment, Institut de Recherche pour le Développement (IRD) UMI 174, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand. 5 Regional Hospital, Chonburi, Thailand. 6 Queen Sirikit National Institute of Child Health, Bangkok, Thailand. 7 Department of Pediatrics, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand. 8 Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. 9 Department of Pediatrics, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. 10 Petchburi Hospital, Petchburi, Thailand. 11 Chiang Rai Regional Hospital, Chiang Rai, Thailand. 12 South East Asia Research Collaboration with Hawaii, Bangkok, Thailand. 13 Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 2 ABSTRACT Objectives: The aim of the study was to assess the prevalence, predictors and patterns of genotypic resistance mutations in children after failure of World Health Organization-recommended initial nonnucleoside reverse transcriptase inhibitor (NNRTI)-based treatment regimens. Methods: We carried out a multicentre retrospective study of genotyping tests performed for all HIV-infected children at eight paediatric centres in Thailand who experienced failure of NNRTI therapy at a time when virological monitoring was not routinely available. Results: One hundred and twenty children were included in the study. Their median age (interquartile range) was 9.1 (6.8-11.0) years, the median duration of their NNRTI regimens was 23.7 (15.7-32.6) months, their median CD4 percentage was 12% (4-20%), and their median plasma HIV RNA at the time of genotype testing was 4.8 (4.3-5.2) log(10) HIV-1 RNA copies/mL. The nucleoside reverse transcriptase inhibitor (NRTI) resistance mutations found were as follows: 85% of the children had M184V/I, 23% had at least four thymidine analogue mutations, 12% had the Q151M complex, 5% had K65R, and 1% had the 69 insertion. Ninety-eight per cent of the children had at least one NNRTI resistance mutation, and 48% had etravirine 39 mutation-weighted scores ≥4. CD4 percentage <15% prior to switching regimens [odds ratio (OR) 5.49; 95% confidence interval (CI) 2.02-14.93] and plasma HIV RNA>5 log(10) copies/mL (OR 2.46; 95% CI 1.04-5.82) were independent predictors of at least four thymidine analogue mutations, the Q151M complex or the 69 insertion. Conclusions: In settings without routine viral load monitoring, secondline antiretroviral therapy regimens should be designed assuming that clinical or immunological failure is associated with high rates of multi-NRTI resistance and NNRTI resistance, including resistance to etravirine. 40 Published in HIV Med. 2010 Oct 1;11(9):565-72. INTER-SPECIFIC GENE FLOW DYNAMICS DURING THE PLEISTOCENE-DATED SPECIATION OF FOREST-DEPENDENT MOSQUITOES IN SOUTHEAST ASIA Katy Morgan1, Yvonne-Marie Linton2, Pradya Somboon3, Prasanta Saikia4, Vas Dev5, Duong Socheat6 and Catherine Walton1 1 Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK. Department of Entomology, Natural History Museum, London, SW7 5BD, UK. 3 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Animal Ecology and Wildlife Biology Laboratory, University of Gauhati, Assam, India. 5 National Institute of Malaria Research, Sonapur, Assam, India 6 National Centre for Malaria, Parasitology and Entomology, Phnom Penh, Cambodia. 2 ABSTRACT Tropical forests have undergone repeated fragmentation and expansion during Pleistocene glacial and interglacial periods, respectively. The effects of this repeated forest fragmentation in driving vicariance in tropical taxa have been well studied. However, relatively little is known about how often this process results in allopatric speciation, since it may be inhibited by recurrent gene flow during repeated secondary contact, or to what extent Pleistocene-dated speciation results from ecological specialization in the face of gene flow. Here, divergence times and gene flow between three closely-related mosquito species of the Anopheles dirus species complex endemic to the forests of Southeast Asia, are inferred using coalescent based Bayesian analysis. An Isolation with Migration model is applied to sequences of two mitochondrial and three nuclear genes, and 11 microsatellites. The divergence of An. scanloni has occurred despite unidirectional nuclear gene 41 flow from this species into An. dirus. The inferred asymmetric gene flow may result from the unique evolutionary adaptation of An. scanloni to limestone karst habitat, and therefore the fitness advantage of this species over An. dirus in regions of sympatry. Mitochondrial introgression has led to the complete replacement of An. dirus haplotypes with those of An. baimaii through a recent (~62 kya) selective sweep. Speciation of An. baimaii and An. dirus is inferred to have involved allopatric divergence throughout much of the Pleistocene. Secondary contact and bidirectional gene flow has occurred only within the last 100,000 years, by which time the process of allopatric speciation seems to have been largely completed. 42 Published in Molecular Ecology (2010) 19: 2269-2285. LATE EFFECTS IN SURVIVORS OF CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA: A STUDY FROM THAI PEDIATRIC ONCOLOGY GROUP Samart Pakakasama1, Gavivann Veerakul2, Darin Sosothikul3, Su-on Chainansamit4, Vichai Laosombat5, Pattra Thanarattanakorn6, Rachata Lumkul7, Surapon Wiangnon8, Somporn Wangruangsathit9, Nattee Narkbunnam2 and Somjai Kanjanapongkul10 1 Department of Pediatrics, Faculty of Medicine, Mahidol University, Rama VI Road, Rajthevi, Bangkok 10400, Thailand. 2 Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand. 3 Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 4 Khon Kaen Hospital, Khon Kaen, Thailand. 5 Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand. 6 Faculty of Medicine, Chiangmai University, Chiangmai, Thailand. 7 Phramongkutklao Hospital, Bangkok, Thailand. 8 Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. 9 Buddhachinaraj Hospital, Phitsanulok, Thailand. 10 Queen Sirikit National Institute of Child Health, Bangkok, Thailand. ABSTRACT Survivors of childhood acute lymphoblastic leukemia (ALL) are at risk of long-term late effects. Therefore, systematic screenings of the late complications are essential. The objective of this study was to determine the prevalence of late effects of Thai children and adolescents after completion of ALL therapy. We performed a cross-sectional study for evaluation of the late effects in ALL survivors who came for follow-up at 10 pediatric oncology centers in Thailand. We evaluated the treatment-related late complications of children and adolescents who had finished ALL treatment for at least 2 years. Demographic data, treatment modalities, and late effects were recorded and analyzed. There were 258 survivors with a median age of 12.2 years (range 3.6-23.3 years). The median follow-up time was 7.2 years (range 2-17.5 years). Forty-seven percent (122 cases) suffered from at least one late effect. Overweight/obesity was the most common late effect. Radiation of central nervous system was a significant risk factor for overweight/obesity (OR 1.97, 95% CI 1.02-3.81) and educational problems (OR 4.3, 95% CI 1.32-14.02). Our data have demonstrated a significant prevalence of late effects after childhood ALL therapy. A long-term follow-up program for survivors of childhood cancer is therefore needed in our country. Published in Int J Hematol. 2010 Jun;91(5):850-4. 43 MEASLES OUTBREAK IN AN ORPHANAGE: HIV-INFECTED CHILDREN ON ANTIRETROVIRAL THERAPY ARE STILL AT RISK Linda Aurpibul1, Thanyawee Puthanakit1, Suparat Kanjanavanit2, Thira Sirisanthana1 and Virat Sirisanthana3 1 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Chiang Mai Provincial Hospital, Chiang Mai, Thailand. 3 Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT An outbreak of measles occurred in an orphanage in Chiang Mai, Thailand where 44 HIV-infected and 19 HIV-uninfected children were accommodated. History of measles vaccination was significantly correlated with the risk of acquiring measles, where HIV infection status was not. 44 Published in Pediatr Infect Dis J. 2010 Feb;29(2):167-9. MEDIASTINAL PANCREATIC PSEUDOCYST IN CHRONIC PANCREATITIS Pannee Visrutaratna1 and Nuthapong Ukarapol2 1 Department of Radiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A 14-year-old girl with chronic pancreatitis presented with palpitations, dysphagia, and pain in the subxyphoid region radiating to her back for 3 months. A contrast-enhanced CT showed a large cystic mass in the mediastinum compressing the heart and esophagus (Fig. 1, arrow). It arose from the pancreas, extending into the mediastinum through the esophageal hiatus (Fig. 2). Note pancreatic calcifications (Fig. 2, arrow). Fig. 1 Axial CT Fig. 2 CT with coronal reformation Pancreatic pseudocysts are complications of acute or chronic pancreatitis; they can extend well beyond the pancreas. The most common route of dissection of a mediastinal pancreatic pseudocyst is through the esophageal or aortic hiatus. Rarely, communication can occur via the foramen of Morgagni or by direct erosion through the diaphragm [1]. Complications of mediastinal pancreatic pseudocyst include infection and hemorrhage, as well as compression, invasion, and rupture of the pseudocyst into surrounding structures [2]. Published in Pediatr Radiol. 2010 Jul;40(7):1298. Epub 2009 Oct 2. 45 MULTIPLEX ASSAY TO IDENTIFY KOREAN VECTORS OF MALARIA Deepak Joshi1, Mi-Hyun Park1, Atiporn Saeung2, Wej Choochote2 and Gi-Sik Min1 1 Department of Biological Sciences, Inha University, Incheon 402-751, Korea. Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Following the recent emergence of malaria in South Korea, vector control has been an important task. For this, vector identification is very important. Earlier, two PCR-based assays have been described. But, poor species resolution and their ability to include only 4-5 species limit their use. Thus, it has now become important to revise the assay identifying these members. In this study, a new assay based on internal transcribed spacer 2 and 28S of ribosomal DNA has been described. The assay successfully identified all the Korean malaria vector mosquitoes. Therefore, it is an indispensable tool to study ecology, abundance and biology of these species. 46 Published in Molecular Ecology Resources (2010) 10: 748-750. PERSISTENCE OF MEASLES, MUMPS, AND RUBELLA PROTECTIVE ANTIBODIES 3 YEARS AFTER REVACCINATION IN HIV-INFECTED CHILDREN RECEIVING ANTIRETROVIRAL THERAPY Linda Aurpibul1, Thanyawee Puthanakit1, Thira Sirisanthana1 and Virat Sirisanthana2 1 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Three years after measles, mumps, and rubella revaccination in 38 human immunodeficiency virus-infected children who had achieved immune recovery after antiretroviral therapy, the prevalence of protective antibody levels was 85% for measles, 61% for mumps, and 79% for rubella, compared with 88%, 84%, and 100%, respectively, 1 month after revaccination. Published in Clin Infect Dis. 2010 May 15;50(10):1415-8. 47 PERSISTENT HYPERINSULINEMIC HYPOGLYCEMIA OF INFANCY ASSOCIATED WITH CONGENITAL NEUROBLASTOMA: A CASE REPORT Prapai Dejkhamron1, Kevalee Unachak1, Pattra Thanarattanakorn1, Pimlak Charoenkwan1, Watcharee Tantiprabha1, Somporn Chotinaruemol1 and Benjaporn Chaiwun2 1 Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The authors report a rare case of persistent hyperinsulinemic hypoglycemia of infancy (PHHI) with congenital neuroblastoma without feature(s) of BeckwithWiedemann syndrome. A term newborn with a birth weight of 3,900 g developed hypoglycemia one hour after birth and required up to 20 mg/kg/min of intravenous glucose infusion to maintain euglycemia. Investigations during the critical period revealed an inappropriately high insulin level. An abdominal CT scan revealed a normal pancreas, right suprarenal mass, and liver nodules. A condition of stage 4S neuroblastoma was suspected and supported by an increased ratio of urine vanillylmandelic acid to creatinine. The bone marrow smear was normal. She underwent near total pancreatectomy at the age of 2 months. The suprarenal mass and liver nodules were not found during the operation or during repeated abdominal CT scans at 3 month of age. Spontaneous regression of neuroblastoma was suspected. The pathology of the pancreas was compatible with PHHI. 48 Published in J Med Assoc Thai. 2010 Jun;93(6):745-8. POOR COGNITIVE FUNCTIONING OF SCHOOL-AGED CHILDREN IN THAILAND WITH PERINATALLY ACQUIRED HIV INFECTION TAKING ANTIRETROVIRAL THERAPY Thanyawee Puthanakit1, Linda Aurpibul1, Orawan Louthrenoo2, Pimmas Tapanya3, Radchaneekorn Nadsasarn1, Sukrapee Insee-ard1 and Virat Sirisanthana2 1 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Faculty of Humanities, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Neurocognitive outcome is an essential aspect of treatment for HIV-infected children. This study is aimed at assessing cognitive functioning in school-aged HIV-infected children and the change after receiving antiretroviral therapy (ART). We conducted a prospective cohort study of HIV-infected Thai children from 6-12 years of age compared with HIV-affected (children of HIV-positive mothers who were not infected with HIV), and normal control groups. Wechsler Intelligence Scale for Children-III (WISC-III) was administered at enrollment and 30 months of followup. Semistructured interviews of primary caregivers were performed. From April to October 2003, 121 children were enrolled; 39 HIV-infected, 40 HIV-affected, and 42 control children with a median age of 9.3 years. The HIV-infected group had a mean (standard deviation [SD]) CD4 percentage of 13.8% (5.3), 87% of whom had been receiving ART for a median of 35 weeks. At the first cognitive assessment, the mean (SD) of full-scale intelligence quotient (FSIQ) was 79 (13) and 88 (10) among HIV-infected and HIV-affected children, which was statistically lower than that of the control group at 96 (13; p < 0.01). The proportion of children with average intelligence level (FSIQ > 90) among 3 groups were 21%, 49%, and 76%, respectively (p < 0.01). At 30 months of follow-up, the HIV-infected group had a mean (SD) CD4 percentage of 25.6% (5.6); 77% had undetectable viral load. The mean (SD) FSIQ of children among three groups were 75 (12), 85 (12), and 91 (12), respectively. Compared with the baseline assessment, the verbal scale score significantly decreased in all groups, including the controls, whereas the performance scales did not change. In conclusion, school-aged HIV-infected children have lower cognitive function than HIV-affected and normal children. Cognitive function was not improved after receiving ART. Further study to address whether early ART can preserve cognitive functioning among HIV-infected children should be explored. Published in AIDS Patient Care STDS. 2010 Mar;24(3):141-6. 49 Predictors and consequences of incarceration among a sample of young Thai methamphetamine users Sherman S.G.1, Sutcliffe C.G.1, Srirojn B.3, German D.1, Thomson N.2, Aramrattana A.2 and Celentano D.D.1 1 Johns Hopkins University Bloomberg School of Public Health, Baltimore, USA. Department of Family Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Introduction and Aims. Our study examined the incidence of arrest and incarceration, investigated predictors of incarceration and explored the persistence of depression, alcohol use and drug use after incarceration among young methamphetamine users from Thailand. Design and Methods. Participants were aged 18-25 years old and were a part of a 12 month randomized social network trial that aimed to reduce sexual risk and methamphetamine use. Estimates of the incidence of arrest and incarceration over 12 months were calculated. A matched case-control study (n = 73 cases; n = 223 controls) was performed to examine incarceration risk factors using conditional logistic regression. Persistence of drug-risk behaviours were explored after incarceration. Results. Study participants (n = 950) were 72% male, with a median age of 19 years and a median of 9 years of schooling. Frequent drug and alcohol use were reported at baseline. In total, 35% of the sample reported ever having been arrested and 22% reported ever having been incarcerated at baseline. During the 12 month follow up, 16% of the sample was arrested. In univariate analyses, risk factors for incarceration included frequent drug and alcohol use, being less educated, and a history of arrest and incarceration. A high prevalence of drug and alcohol use and involvement in the drug economy persisted after arrest. Discussion and Conclusions. The study indicates a high prevalence of recidivism among this young sample, with continued involvement in drug-risk behaviours after incarceration. Appropriate interventions are needed to address root causes of arrest, largely related to substance use. 50 Published in drug and Alcohol Review 29 (4) 2010, pp.399-405. PRODUCTION OF MONOCLONAL ANTIBODIES TO P-GLYCOPROTEIN: ITS APPLICATION IN DETECTION OF SOLUBLE AND SURFACE P- LYCOPROTEIN OF LEUKEMIA PATIENTS Sawitree Chiampanichayakul1,2, Songyot Anuchapreeda1, Nuttapol Chruewkamlow2, Kodchakorn Mahasongkram2, Pattra Thanaratanakorn3 and Watchara Kasinrerk2 1 Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Pediatric, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Multidrug resistance (MDR) in leukemia is commonly associated with the expression of a transmembrane protein, P-glycoprotein (P-gp). In this study, two monoclonal antibodies (mAbs) specific for the extracellular domain of P-gp were generated. By employing the generated mAbs, a two-color lysed whole blood flow cytometric method for surface P-gp and an efficient sandwich ELISA for soluble P-gp determinations were established. By using the established methods, surface and soluble P-gp were detected in several leukemia patients. The presence of soluble P-gp could be used to identify the P-gp surface expression patients. Detection of soluble P-gp reported provides a new basis that may lead to a better understanding of the MDR mechanism in leukemia. Published in Int J Hematol. 2010 Sep;92(2):326-33. 51 RAPID DETECTION OF DIROFILARIA IMMITIS IN MOSQUITO VECTORS AND DOGS USING A REAL-TIME FLUORESCENCE RESONANCE ENERGY TRANSFER PCR AND MELTING CURVE ANALYSIS Tongjit Thanchomnang1, Pewpan M. Intapan1, Viraphong Lulitanond2, Somboon Sangmaneedet3, Sudchit Chungpivat4, Piyanan Taweethavonsawat4, Wej Choochote5 and Wanchai Maleewong1 1 Department of Parasitology, Faculty of Medicine and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. 2 Department of Microbiology, Faculty of Medicine and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. 3 Department of Pathology, Faculty of Veterinary Science, Khon Kaen University, Khon Kaen 40002, Thailand. 4 Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. 5 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors. 52 Published in Veterinary Parasitology (2010) 168: 255-260. RELATIONSHIP OF CELL BEARING EBER AND p24 ANTIGENS IN BIOPSY-PROVEN LYMPHOCYTIC INTERSTITIAL PNEUMONIA IN HIV-1 SUBTYPE E INFECTED CHILDREN Lertlakana Bhoopat1, Somrak Rangkakulnuwat2, Charin Ya-In1 and Tanin Bhoopat3 1 Departments of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Departments of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Departments of Forensic Medicine Molecular Laboratory, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Lymphocytic interstitial pneumonia (LIP) is an uncommon histopathologic entity characterized by infiltration of the interstitium and alveolar spaces of the lung by lymphocytes and other lymphoid elements. An increased incidence of LIP has been seen in the pediatric population, especially in children with acquired immune deficiency syndrome. Our previous study supports the notion that Langerhans cells (LCs) are reservoirs for Epstein-Barr virus (EBV) in lungs of human immunodeficiency virus (HIV) subtype E-infected pediatric LIP. To further understand the pathogenesis of LIP, we studied the relationship between EBV, the suggested causative agent of LIP and HIV-1 capsid protein p24, which play an important role in the interaction with host proteins during HIV-1 adsorption, membrane fusion, and entry in surgical lung biopsy-proven LIP from 9 vertically HIV subtype E-infected pediatric patients. The dominant microscopic feature of LIP demonstrated widespread widening of alveolar septum by mononuclear inflammatory cell infiltrate, mainly composed of mature lymphocytes and plasma cells surrounding airways and expanding to the lung interstitium. EBV-encoded RNA (EBER) in situ hybridization (ISH) and p24 immunohistochemistry, performed on formalin-fixed, paraffin-embedded tissue from open lung biopsy specimens, revealed positive intranuclear EBER signals and intracytoplasmic immunostains for p24 core protein in all 9 LIP cases. By combining ISH and immunohistochemistry, these results suggest that (i) EBV/p24-carrying cells are likely involved in the development of LIP, either directly or indirectly; (ii) LCs and related dendritic cells are the main 53 reservoir of both EBV and HIV subtype E in pediatric LIP and possibly LCs may play an important role in the recruitment of inflammatory cell infiltrates, especially T cells into these tissues; (iii) coexpression of EBV/p24 in bronchioalveolar epithelium supports the hypothesis that these cells serve as a reactivation source for both viruses to achieve greater quantities in alveolar septum and interstitium around bronchioles. These results indicate a strong association between the presence of HIV core protein p24 and expression of EBV RNA transcripts (EBER). Interactions between LCs and related dendritic cells together with T cells are important for effective HIV and EBV replications. The coexpression of both viruses could be related to the evolution of pediatric LIP in HIV subtype E infection. 54 Published in Appl Immunohistochem Mol Morphol. 2011 May 26. REPORT OF THE 11TH WORLD CONGRESS OF PEDIATRIC DERMATOLOGY IN BANGKOK, THAILAND Chulabhorn Pruksachatku Division of Dermatology, Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT th The 11 World Congress of Pediatric Dermatology took place at the Queen Sirikit National Convention Center, Bangkok, Thailand on 17 to 19 November 2009. The theme was “Health and Beauty in Pediatric Dermatology.” This report highlights several presentations discussing hemangiomas, infections, and novel and future diagnostic methods and treatment of pediatric dermatologic conditions. This report is not intended as a substitute for reading the conference educational handouts, online updates and related references quoted in this article. Published in Pediatr Dermatol. 2010 May-Jun;27(3):223-5. 55 SIZE AND CHARGE ANTIGENS OF DIROFILARIA IMMITIS ADULT WORM FOR IGG-ELISA DIAGNOSIS OF BANCROFTIAN FILARIASIS Doungrat Riyong1, Jitra Waikagul2, Chotechuang Panasoponkul3, Wej Choochote1, Akira Ito4 and Paron Dekumyoy2 1 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. 3 Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. 4 Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido, Japan. ABSTRACT We used Dirofilaria immitis adult worm antigens to develop an IgG-ELISA, then used this to evaluate 30 serum samples of patients with proven Wuchereria bancrofti infection, 131 samples of patients with other parasitic diseases and 30 serum samples of healthy controls. The D. immitis antigen was prepared using two methods: Sephacryl S-200 chromatography and iso-electric focusing with a Rotofor cell. This was done to determine the best method for diagnosing W. bancrofti filariasis. Before fractionation, crude male D. immitis antigen yielded 100% sensitivity and 60.8% specificity, and crude female antigen yielded 80% sensitivity and 52.8% specificity, respectively, to detect W. bancrofti infection. After gel filtration chromatography, the male D. immitis antigen, called MP1, yielded 100% sensitivity and 95% specificity, and female D. immitis antigen, called FmP1, gave 100% sensitivity and 59.6% specificity, to detect W. bancrofti infection. Using isoelectric-focusing, both male and female crude D. immitis antigens (Iso-MF and Iso-FmF, respectively) were separated mechanically into 20 iso-fractions (F1-F20) each. By preliminary screening with ELISA, using pooled positive and negative sera, Iso-MF10, pH 7.5, and Iso-FmF14, pH 7.6, were selected. Iso-MF10 gave 100% sensitivity and 96.9% specificity, and Iso-FmF14 gave 100% sensitivity and 64% specificity. In the study, Og4C3-ELISA, for the detection of circulating filarial antigen, was also used to analyze these serum samples, it gave 87.6% sensitivity and 99.4% specificity to detect W. bancrofti infection. Male D. immitis antigens, MP1 and Iso-MF10, gave high sensitivity and specificity, and appear to be the best choices for use in an ELISA to diagnose bancroftian filariasis. Published in Southeast Asian Journal of Tropical Medicine and Public Health (2010) 41(2): 285-97. 56 SPATIAL GENETIC STRUCTURE OF AEDES AEGYPTI MOSQUITOES IN MAINLAND SOUTHEAST ASIA Thaung Hlaing1, Willoughby Tun-Lin1, Pradya Somboon2, Duong Socheat3, To Setha3, Sein Min1, Sein Thaung1, Okorie Anyaele4, Babaranda De Silva5, Moh Seng Chang6, Anil Prakash7, Yvonne Linton8 and Catherine Walton9 1 Medical Entomology Research Division, Department of Medical Research (Lower Myanmar), Yangon, Myanmar. 2 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. 3 National Centre for Malaria, Parasitology and Entomology, Phnom Penh, Cambodia. 4 Entomology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria. 5 Department of Zoology, University of Sri Jayewardenepura, Nugegoda, Sri Lanka. 6 WHO - Western Pacific Regional Office, Phnom Penh, Cambodia. 7 Regional Malaria Research Centre, Indian Council of Medical Research, Dibrugarh, Assam, India 8 Natural History Museum, London, UK. 9 Faculty of Life Sciences, University of Manchester, Manchester, UK. ABSTRACT Aedes aegypti mosquitoes originated in Africa and are thought to have spread recently to Southeast Asia, where they are the major vector of dengue. Thirteen microsatellite loci were used to determine the genetic population structure of A. aegypti at a hierarchy of spatial scales encompassing 36 sites in Myanmar, Cambodia and Thailand, and two sites in Sri Lanka and Nigeria. Low, but significant, genetic structuring was found at all spatial scales (from 5 to >2000 km) and significant FIS values indicated genetic structuring even within 500 m. Spatially dependent genetic-clustering methods revealed that although spatial distance plays a role in shaping larger-scale population structure, it is not the only factor. Genetic heterogeneity in major port cities and genetic similarity of distant locations connected by major roads, suggest that human transportation routes have resulted in passive long-distance migration of A. aegypti. The restricted dispersal on a small spatial scale will make localized control efforts and sterile insect technology effective for dengue control. Conversely, preventing the establishment of insecticide resistance genes or spreading refractory genes in a genetic modification strategy would be challenging. These effects on vector control will depend on the relative strength of the opposing effects of passive dispersal. Published in Evolutionary Applications (2010) 3: 319-339. 57 SYSTEMATICS OF ANOPHELES (CELLIA) YAEYAMAENSIS SP. N., ALIAS SPECIES E OF THE AN. MINIMUS COMPLEX IN SOUTHEASTERN ASIA (DIPTERA: CULICIDAE) Pradya Somboon1, Angela Rory2, Yoshio Tsuda3, Masahiro Takagi4 and Ralph E. Harbach2 1 Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Entomology, The Natural History Museum, Cromwell Road, London SW7 5BD, U.K. 3 Department of Medical Entomology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. 4 Center for International Collaborative Research, Nagasaki University, Sakamoto 1-12-4, Nagasaki City, Nagasaki 852-8523, Japan. ABSTRACT Species E of the Anopheles minimus sibling species complex is diagnosed and formally named An. yaeyamaensis Somboon & Harbach, sp. n. Information is provided on the morphology, genetics and bionomics of the species, which has a restricted distribution in the Yaeyama and Miyako Islands located at the southern end of the Ryukyu Archipelago of Japan. 58 Published in Zootaxa (2010) 2651: 43-51. The Thai Anesthesia Incident Monitoring Study (Thai AIMS): an analysis of perioperative complication in geriatric patients Tuchinda L.1, Sukchareon I.2, Kusumaphanyo C.3, Suratsunya T.4, Hintong Thanoo5 and Thienthong S.6 1 Department of Anesthesiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 2 Department of Anesthesiology, Bhumibol Adulyadej Hospital, Bangkok, Thialand. 3 Department of Anesthesiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand. 4 Department of Anesthesiology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand. 5 Department of Anesthesiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. 6 Department of Anesthesiology, Faculty of Medicince, Khon Kaen University, Khone Kaen, Thailand. ABSTRACT Background: The present study was a part of the multi-centered study of model of Anesthesia related adverse events in Thailand by incident report. (The Thai Anesthesia Incident Monitoring Study or Thai AIMS). The objective of the present study was to identify and analyze anesthesia incident in geriatric patients in order to find out the frequency distribution, clinical courses, management of incidents and investigation of model appropriate for possible corrective strategies. Material and Method: This study was a prospective descriptive multicentered study conducted between January 1, 2007 and June 30, 2007. Incident reports from 51 hospital across Thailand were sent to data management unit on anonymous and voluntary basis. The authors extracted relevant data from the incident reports on geriatric patients (age 65 or more). The cases were reviewed by 3 anesthesiologists. Any disagreement was discussed and judged to achieve a consensus. Descriptive statistics was used. Results: Among 407 incident reports and 559 incidents, there were more male (52.8%) than female (46.7%) patients with ASA PS 2, 3, 4 and 5 = 38.6%, 42.8%, 14.5% and 4.2% respectively. Surgical specialties that posed high risk of incidents were general, orthopedic, neurological, urologic and otorhiolaryngological surgery. Common places where incidents occurred were operating room (57.1%), ward (30.9%) and recovery room (12.0%). Common occurred incidents 59 were arrhythmia needing treatment (30.0%), death within 24 hr (24.6%), desaturation (21.9%), cardiac arrest (16.2%) and reintubation (16.0%). The causes of the incidents were mostly attributed from patients underlying diseases and conditions. Most common outcomes were major physiologic changes with 26.5% fatal outcome at 7 days. The most common contributing factor was human factor (inappropriate decision and inexperience). Vigilance and having more experience could be the minimizing factors. Conclusion: Incidents in geriatric patients were similar to all age group patients with a higher incidents in death within 24 hr. The outcome were more serious resulting in 26.5% fatal outcome at 7 days. Quality assurance activity, clinical practice guidelines and improved supervision were suggested corrective strategies. 60 Published in J Med Assoc Thai. 2010 Jun;93(6):698-707. Women’s Health survey of female prisoners at Chiang Mai Women Prison Wichuda Jiraporncharoen, Apinun Aramrattana, Nopakoon Koonlertgit and Chaisiri Angkurawaranon Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. abstract Background: Prisoner health still remains a major challenge in many health systems. For female inmates, women’s health issues such as reproductive health, gynecologic disorders and maternal health are often overlooked in these settings. Objectives: Document the reproductive health status of female prisoners in order to plan for proper screening, treatment and prevention measures. Methods: 517 inmates from Chiang Mai Women Prison volunteered to take part in the survey during 2007 by answering questionnaires. Results: the majority of female prisoners (94%) is this survey had already had sexual intercourse and almost all (99%) reported that they did not regularly use condom. 27% were sexually active before the age of 16 while 21% had more than 3 sexual partners. In terms of reproductive health, 81% had been pregnant before and about 36% had a history of abortion while 16% had more than 3 abortions. Only 27.4% of these reported abortions were due to medical indication, the rest were mostly criminal abortions. 49% of female prisoners in this survey reported that they had a positive history of contracting sexually transmitted diseases. (STDs). Conclusions: The majority of female prisoners reported high risk behaviors which is reflected by the fact that almost 50% of prisoners reported that they had a history of contracting STDs. Routing screening and access to treatment is crucial for this high risk group. Health promotion, prevention and harm reduction program is also recommended for all inmates to promote comprehensive women’s health issue in this marginalized but high risk population. Published in Chiang Mai Medical Journal, Vol. 50 No.2, June 11 (p.51-58). 61 Determination of Clozapine in Human Plasma by High–Performance Liquid Chromatography with UV–VIS Detector Chokchai Wongsinsup, Wandee Taesotikul, Sayam Kaewvichit, Siriluk Sangsrijan and Saowarunee Sangsrijan Biopharmacy Research Unit, Pharmaceutical Sciences Research and Service Center, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A specific reversed- phase high-performance liquid chromatographic method has been developed for the simultaneous determination of clozapine in human plasma. Diazepam was used as an internal standard. The drugs from human plasma were extracted by liquid-liquid extraction with diethyl ether. The analysis was performed on a C18 analytical column with UV - VIS detector at 250 nm and acetonitrile-methanol-0.5% triethylamine (40:10:50) was used as mobile phase. It was found to be linear linear over the concentration range of 25 to 2000 ng/ml and extraction recovery was more than 80%. The coefficients of variation (CV) for intraday and interday assay were found to be less than 5%. The limit of quantification (LOQ) was 25 ng/ml. This analysis method was successfully used in pharmacokinetic and bioequivalence study of clozapine in schizophrenic patients. Key words: Clozapine, Plasma analysis, HPLC, Determination, Pharmacokinetic Published in Chiang Mai University Journal of Natural Sciences. 9(1): 29-38. JanuaryJune 2010. 62 Inhibitory effects of caffeic acid ester analogues on free radicals and human liver microsome CYP1A2 activities Churdsak Jaikang1, Chaiyavat Chaiyasut1, Paitoon Narongchai2, Kanokporn Niwatananun3, Siripun Narongchai2 and Winthana Kusirisin4 1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Thailand. Department of Foriensic Medicine, Faculty of Medicine, Chiang Mai University, Thailand. 3 Department of Pharmaceutical Care, Faculty of Pharmacy, Chiang Mai University, Thailand. 4 Department of Family Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Ethyl caffeate (EC), octyl caffeate(OC), benzyl caffeate(BC) and phenethyl caffeate(PC) were synthesized and evaluated for scavenging of superoxide anion, nitric oxide radical and 1,1-diphenyl-1-picrylhydrazyl radical (DPPH). Antioxidant activity was investigated with reducing power method. Pooled human liver microsome was used for investigating the effects on cytochrome P450 1A2 (CYP1A2) catalytic activities by using phenacetin as a substrate. Dixon and Cornish-Bowden plots were used for enzyme kinetic analysis. The EC, OC, BC and PC potentially inhibited superoxide anion, nitric oxide and DPPH radicals. IC(50) values of superoxide anion scavenging of EC, OC, BC and PC were 16.42, 79.83, 123.69 and 123.69 µg/ml, respectively. EC was more potent than OC and BC in terms of nitric oxide radical scavenger: IC(50) values of EC, OC and BC were 24.16, 37.34 and 52.64 µg/ml, respectively. In addition, the IC(50) values of EC, OC, BC and PC on DPPH radical scavenging were 70.00, 184.56, 285.34 and 866.54 µg/ ml, respectively. The IC(50) values of EC, OC, BC and PC on phenacetin O-deethylation were 124.98, 111.86, 156.68 and 31.05 µg/ml, respectively. Enzyme kinetics showed that the type of inhibition mechanism was mixed-type. The result of this study shows that caffeic acid ester analogues potentially scavenge free radicals and inhibit catalytic activity of CYP1A2. This may lead to important implications in the prevention of CYP1A2-mediated chemical carcinogenesis. Published in the Medicinal Chemistry. 7(2): 99-105. 1 Mar 2011. 63 A MICROFLOW CHEMILUMINESCENCE SYSTEM FOR DETERMINATION OF CHLORAMPHENICOL IN HONEY WITH PRECONCENTRATION USING A MOLECULARLY IMPRINTED POLYMER Wisanu Thongchai1, Boonsom Liawruangath1,4, Saisunee Liawruangrath2,4 and Gillian M. Greenway3 1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, University of Hull, Hull HU6 7RX, United Kingdom. 4 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A novel chemiluminescence (CL) microfluidic system incorporating a molecularly imprinted polymer (MIP) preconcentration step was used for the determination of chloramphenicol in honey samples. The MIP was prepared by using chloramphenicol as the template, diethylaminoethyl methacrylate (DAM) as the function monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking monomer, 2, 2’-dimethoxy-2 phenylacetophenone (DMPA) as the free radical initiator and toluene and dodecanol as the solvent. TheMIPwas pre-loaded into a 10mmlong, 2mmwide and 150mdeep channel in a planar glass microfluidic device.When the sample containing chloramphenicol was introduced into the microfluidic device it was first preconcentrated on the MIP then detected by an enhancement effect on the chemiluminescence reaction of tris(2, 2’-bipyridyl) ruthenium(II) with cerium(IV) sulphate in sulphuric acid. A micro-syringe pump was used to pump the reagents. The CL intensity was linear in relationship to the chloramphenicol concentrations from 1.55×10-4 to 3.09×10-3 µmol L-1 (r2 = 0.9915) and the detection limit (3σ) and the quantitation limit (10σ) were found to be 7.46×10-6 and 2.48×10-5 µmol L-1, respectively. Thismethod offered a high selectivity and sensitivity for quantitative analysis of chloramphenicol in the honey samples. 64 Published in the Talanta, 82, pp. 560-566, July 2010. BIOLOGICAL ACTIVITIES OF PHENOLIC COMPOUNDS ISOLATED FROM GALLS OF TERMINALIA CHEBULA RETZ. (COMBRETACEAE) Aranya Manosroi1,2, Pensak Jantrawut1, Hiroyuki Akazaw1,3, Toshihiro Akihisa3 and Jiradej Manosroi1,2 1 Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. Natural Products Research and Development Center, Science and Technology Research Institute, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 3 College of Science and Technology, Nihon University, 1-8 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8308, Japan. 2 ABSTRACT The aqueous extract of galls from Terminalia chebula Retz. (Combretaceae) was fractionated on Diaion and refractionated on octadecyl silica column. Six phenolic compounds were isolated and identified as gallic acid (1), punicalagin (2), isoterchebulin (3), 1,3,6-tri-O-galloyl-_-D-glucopyranose (4), chebulagic acid (5) and chebulinic acid (6). All of the compounds showed stronger 2,2-diphenyl1-picrylhydrazyl (DPPH) radical scavenging and melanin inhibitory activities than ascorbic acid, butylated hydroxytoluene, _-tocopherol, arbutin and kojic acid, the reference compounds. Gallic acid (1) exhibited inhibitory activity against nitric oxide production in lipopolysaccharide-activated macrophages. However, all isolated compounds exhibited less activity than the reference compounds in mushroom tyrosinase inhibition and human tumour cytotoxicity assays. This study has demonstrated that the phenolic compounds isolated from galls of T. chebula might contribute significantly due to their antioxidant and whitening activities. Published in the Natural Product Research, 24, pp. 1915-1926, December 2010. 65 BIOLOGICAL ACTIVITIES OF THE RICE BRAN EXTRACT AND PHYSICAL CHARACTERISTICS OF ITS ENTRAPMENT IN NIOSOMES BY SUPERCRITICAL CARBON DIOXIDE FLUID Aranya Manosroi1,2, Warintorn Ruksiriwanich1, Masahiko Abe3, Hideki Sakai3, Worapaka Manosroi4 and Jiradej Manosroi1,2 1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Chiba, Japan. 4 Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The objectives of this study were to compare the biological activities of the rice bran (Oryza sativa Linn.) extracts prepared by supercritical carbon dioxide fluid (scCO2) and ethanolic maceration, and the physical properties of niosomes entrapped with the extracts prepared by scCO2 and chloroform film method. The scCO2 extract showed higher unsaturated fatty acid and phenolic contents than the maceration extract. All extracts showed no significant difference of biological activities. The characteristics of niosomes entrapped and not entrapped with the extract prepared by scCO2 and chloroform film method were not different with a unilamellar nano-sized structure and the phase transition temperatures at 80°C. The gradual decrease trend of the vesicular membrane microviscosity with less membrane rigidity at higher extract concentrations was observed. The scCO2 technique is advantageous for the preparation of the rice bran extracts and niosomes because of no requirement of organic solvents and having less step. 66 Published in the Journal of Supercritical Fluids, 54, pp. 137-144, August 2010. EFFECTS OF VIBRO-MILLING ON RELAXOR FERROELECTRIC BEHAVIOR AND PHASE TRANSITION OF LEAD-FREE Ba(Zr0.25Ti0.75)O3 CERAMICS Chatchai Kruea-In1, Sukum Eitssayeam1, Kamonpan Pengpat1, Gobwute Rujijanagul1,2 and Tawee Tunkasiri1,3 1 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand. ABSTRACT Lead-free Ba(Zr0.25Ti0.75)O3 ceramics were synthesized from powders prepared by a conventional mixed oxide and vibro-milling method to investigate the phase transition, dielectric response, and ferroelectric properties of the prepared samples. Compared to a conventional sample, the samples prepared by the vibro-milling method showed a higher dielectric constant at the ferroelectric-toparaelectric transition temperature. The vibro-milling method also produced a stronger frequency dependence on the dielectric constant. To confirm the dielectric properties and phase transition behavior, ferroelectric hysteresis measurements were carried out in the temperature range 40°C to 80°C. The microstructural properties of the samples were investigated and the results were then correlated with the characteristics of the milled and calcined powder as well as the grains of the ceramics. Published in the Phase Transitions, 83, pp. 942-949, October 2010. 67 ENHANCEMENT OF TRANSDERMAL ABSORPTION GENE EXPRESSION AND STABILITY OF TYROSINASE PLASMID (pmel34)-LOADED ELASTIC CATIONIC NIOSOMES: POTENTIAL APPLICATION IN VITILIGO TREATMENT Jiradej Manosroi1,2, Narinthorn Khositsuntiwong1, Worapaka Manosroi3, Friedrich Götz4, Rolf G. Werner5 and Aranya Manosroi1,2 1 Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University Chiang Mai 50200, Thailand. 3 Faculty of Medicines, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Faculty of Biology, Department of Microbial Genetics, University of Tübingen, Tübingen, Germany. 5 Boehringer Ingelheim Company, Ingelheim am Rhein, Germany. 2 ABSTRACT The pMEL34 was loaded in elastic cationic niosomes (Tween61/Cholesterol/ DDAB at 1:1:0.5 molar ratio) by chloroform film method with sonication and rehydrated with 25% ethanol. The amount of pMEL34 was determined by gel electrophoresis and gel documentation. The maximum loading of pMEL34 in elastic cationic niosomes was 150 microg/16 mg of the niosomal compositions. At 8 weeks, the remaining plasmid in the elastic niosomes kept at 4 +/- 2 degrees C, 27 +/- 2 degrees C were 49.75% and 38.57%, respectively, whereas at 45 +/- 2 degrees C, all plasmids were degraded. For transdermal absorption through rat skin investigated by Franz diffusion cells at 6 h, the fluxes of pMEL34 loaded in elastic and nonelastic niosomes in viable epidermis and dermis (VED) were 0.022 +/- 0.00 and 0.017 +/- 0.01 microg/cm(2)/h, respectively, whereas only pMEL34 loaded in elastic cationic noisome was observed in the receiver solution. The pMEL34 loaded in elastic cationic niosomes showed the highest tyrosinase gene expression demonstrating higher tyrosinase activity than the free and the loaded plasmid in nonelastic niosomes of about four times. This study has suggested the potential application of elastic cationic niosomes as an efficient topical delivery for tyrosinase gene in vitiligo therapy. 68 Published in the Journal of Pharmaceutical Sciences, 99, pp. 3533-3541, August 2010. ENTRAPMENT ENHANCEMENT OF PEPTIDE DRUGS IN NIOSOMES Aranya Manosroi1,2, Penpan Khanrin2, Rolf G. Werner3, Friedrich Gtz4, Worapaka Manosroi5 and Jiradej Manosroi1,2 1 Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI) 2 Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand. 3 Boehringer Ingelheim International GmbH; Ingelheim am Rhein, Germany. 4 Department of Microbial Genetics, Faculty of Biology, University of Tübingen, Tübingen, Germany. 5 Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. ABSTRACT The objective of this study was to enhance the entrapment of various charged peptide drugs [(bacitracin (BCT), insulin and bovine serum albumin (BSA)] in niosomes by modifying the vesicular charge compositions. Cationic, anionic and neutral niosomes were prepared from sorbitan monostearate (Span 60) orpolyoxyethylene sorbitan monostearate (Tween 61), cholesterol (CHL), dimethyldioctadecylammonium bromide (DDAB) and/or dicetyl phosphate (DP) in distilled water, by freeze dried empty liposome (FDEL) method. Morphology and vesicular sizes of the vesicles were investigated by optical microscope, TEM, X-ray diffractometry and dynamic light scattering. The entrapment efficiency of the peptides in niosomes was determined by gel electrophoresis and gel documentation. After reconstitution of the empty niosomal powder in phosphate buffer pH 7.0 containing the peptide drugs, they were oligolamellar membrane structure, with the sizes of 40-60 nm, except the neutral niosomes entrapped with insulin and cationic niosomes entrapped with BSA which showed the sizes of 0.1-1.3 mm and 100-150 nm, respectively. The zeta potential values of neutral, cationic and anionic niosomes entrapped with BSA, insulin and BCT were -22.3±1.52, -30.7±2.92 and +22.68±1.31 mV, respectively. The entrapment efficiency of BSA, BCT and insulin in neutral niosomes (Tween 61/CHL at 1 : 1 molar ratio) was 72.94, 69.89 and 10.26%, in cationic niosomes (Tween 61/CHL/DDAB at 1 : 1 : 0.05 molar ratio) was 84.54, 32.85 and 87.15% and in anionic niosomes (Tween 61/CHL/DP at 1 : 1 : 0.05 molar ratio) was 50.13, 90.88 and 44.31%, respectively. The highest entrapment efficiency of BSA, BCT and insulin at 72.94, 90.88 and 87.15 was observed in neutral, anionic and cationic niosomes, respectively. The results from this study has suggested the appropriate niosomal formulation to entrap the peptides with different charges and polarity for pharmaceutical application. Published in the Journal of Microencapsulation, 27, pp. 272-280, May 2010. 69 FREE RADICAL ACAVENGING AND TYROSINASE INHIBITION ACTIVITY OF OILS AND SERICIN EXTRACTED FROM THAI NATIVE SILKWORMS (BOMBYX MORI) Aranya Manosroi1,2, Korawinwich Boonpisuttinant1, Supanida Winitchai3, Worapaka Manosroi4 and Jiradej Manosroi1,2 1 Division of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand. 2 Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University, Chiang Mai, Thailand. 3 Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok, Thailand. 4 Faculty of Medicine, Chiang Mai University, Thailand. ABSTRACT Oils and sericin were extracted from pupae and silk cocoons, respectively, of the five Thai native silkworms (Bombyx mori, Linnaeus (Bombycidae)), namely, Keawsakol, Nangnoi, Somrong, Nangleung, and Noneruesee, which are variations of the same species. The oils were extracted by a hot process using Soxhlet apparatus and a cold process using petroleum ether, while sericin was extracted by basic hydrolysis and autoclaving. Sericin from the five Thai native silkworms showed free radical scavenging activity lower than the standard antioxidants (vitamin C, vitamin E, and BHT) by about 20-100-fold, but all oils gave higher activity than that of the standard linoleic acid by 11-22-fold. Oil extracted from Noneruesee by the cold process gave the highest DPPH scavenging activity, compared with other oil samples. All sericin samples showed tyrosinase inhibition activity with IC50 values in the range of 1.2-18.76 mg/mL, but only oils from Noneruesee extracted by the hot process, and Nangleung, Somrong, and Noneruesee extracted by the cold process, showed this activity. Oil extracted by the hot process and sericin by basic hydrolysis from Noneruesee gave the highest tyrosinase inhibition activity, but lower than that of the standards vitamin C and kojic acid by 20-49 and 3-8 times, respectively. This study has suggested that sericin and oil from Noneruesee extracted by basic hydrolysis and the cold process, which gave the highest tyrosinase inhibition and free radical scavenging activity, respectively, can be applied in antiaging and whitening cosmetic products. 70 Published in the Pharmaceutical, 48, pp. 855-860, August 2010. HARMONIC ANALYSIS OF DYNAMIC HYSTERESIS RESPONSE OF BaTiO3 BULK CERAMICS Kanokwan Kanchiang1,2, Rattikorn. Yimnirun3, Natthapong Wongdamnern1, Athipong Ngamjarurojana1,2 and Yongyut Laosiritaworn1,2,4 1 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ThEP Center, CHE, 328 Si Ayutthaya Road, Bangkok, 10400, Thailand. 3 School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. 4 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT In this work, the dynamic ferroelectric hysteresis properties in response to dynamic electric field of BaTiO3 bulk ceramics was investigated using the harmonic analysis approach. Fourier transformation was used to analyze the periodic polarization signal on frequency domain via each discrete harmonic. From the results, the hysteresis area is found to depend only on the first harmonic of the real part. On the other hand, the remnant polarization depends on all odd harmonics of the real part. Further, the coercive field can be found from the phase-lag between the inverse Fourier signals re-calculated from the first harmonic of the real part and that of the imaginary part. The hysteresis properties from the harmonic analysis match well with those of the original measurement. This suggests that the harmonic analysis is one of the powerful techniques which can be used to predict hysteresis behavior. Published in the Ferroelectrics, 401, pp. 123-128, December 2010. 71 HIGH-ENERGY HEAVY ION BEAM ANNEALED ION-IMPLANTATION-SYNTHESIZED SiC NANOCRYSTALLITES AND PHOTOLUMINESCENCEH Jejira Khamsuwan1, Saweat Intarasiri2, Karen Kirkby3, Paul K. Chu4 and Yu Liangdeng1,5 1 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Institute for Science and Technology Research and Development, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Ion Beam Centre, University of Surrey, Guildford, Surrey, GU2 7XH, UK. 4 Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong. 5 Thailand Center of Excellence in Physics, CHE, 328 Si Ayutthaya Road, Bangkok 10400, Thailand. ABSTRACT This work explored a novel way to synthesize silicon carbide (SiC). Carbon ions at tens of keV were first implanted in single crystalline silicon wafers at elevated temperature, followed by irradiation using heavy xenon ion beams at high energy of 4 MeV with fluences of 5×1013 and 1×1014 ions/cm2 at elevated temperatures to play a role of annealing as an alternative of high-temperature thermal annealing. X-ray diffraction, Raman scattering, infrared spectroscopy were used to characterize formation of SiC. Rutherford backscattering spectrometry was used to analyze changes in the carbon depth profiles. Photoluminescence experiment was operated. The results showed that high-energy heavy ion beam annealing could indeed induce crystallization of SiC, mainly depending on the single ion energy but not on the deposited areal density of the ion beam energy (the product of the ion energy and the fluence). The ion beam synthesized SiC could enhance emission of blue-band photoluminescence. Published in the Proceedings on 3rd International Nanoelectronics Conference, pp. 543544, 2010. 72 IN VITRO ANTI-AGING ACTIVITIES OF TERMINALIA CHEBULA GALL EXTRACT Aranya Manosroi1,2, Pensak Jantrawut1, Toshihiro Akihisa3, Worapaka Manosroi4 and Jiradej Manosroi1,2 1 Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand. Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University, Chiang Mai, Thailand. 3 College of Science and Technology, Nihon University, 1-8 Kanda Surugadai, Chiyoda-ku, Tokyo, Japan. 4 Faculty of Medicines, Chiang Mai University, Chiang Mai, Thailand. 2 ABSTRACT Context: The Thai Lanna region has its own folklores and wisdoms in various fields such as traditional medicines. The galls of Terminalia chebula Retz. (Combretaceae) frequently appear in many Thai Lanna medicinal plant recipes for promoting longevity. Objectives: To investigate the in vitro anti-aging activities of the extracts from 15 plants including T. chebula gall selected from the Thai medicinal plant recipes that have been traditionally used for longevity. Materials and methods: The plant extracts were prepared by four extraction methods including hot (HW) and cold (CW) aqueous processes and hot (HM) and cold (CM) methanol processes. These extracts were tested for antioxidative and tyrosinase inhibition activity as well as the proliferative and MMP-2 inhibition activity on early aging human skin fibroblasts in order to evaluate their in vitro anti-aging activity. Results: At 0.1 mg/ mL, the CW extract of T. chebula gall exhibited the highest DPPH radical scavenging activity with scavenging of 84.64%±2.22%, whereas ascorbic acid, α-tocopherol and butylated hydroxyl toluene gave 96.50%±0.1%, 35.74%±0.2% and 27.43%±0.1%, respectively. The CW extract of T. chebula gall indicated the highest stimulation index (SI) on normal human fibroblast proliferation of 1.441 which was more active than ascorbic acid (SI 1.21). This extract has also demonstrated MMP-2 inhibition on fibroblasts determined by zymography 1.37 times more potent than ascorbic acid. Discussion and conclusion: This study has confirmed the traditional use of T. chebula gall in many Thai medicinal plant recipes for longevity which will be beneficial for further development of anti-aging products. Published in the Pharmaceutical Biology, 48, pp. 469-481, April 2010. 73 LEAD-FREE HIGH REFRACTIVE INDEX GLASS USING LOCAL THAI SANDS Pisutti Dararutana1 and Narin Sirikulrat2 1 The Royal Thai Army Chemical Department, Bangkok, 10900 Thailand. Department of Physics, Faculty of Science, Glass and Glass Products Research and Development Laboratory, Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Due to harmful effects of lead in the high refractive index glass processing, the production of lead-free high refractive index glass by using other materials to replace lead has been studied. In this work, local sand from various sites in Thailand was used to fabricate the lead-free high refractive index glass. Barium carbonate was used to replace lead oxide in order to reduce the amount of lead in glass. Results from the experiments found that the local sands and the processes for making glass were satisfactory. The refractive index of greater than 1.60 was found in the glass specimen prepared from a concentration of barium carbonate of more than 30 weight-%. Published in the Songklanakarin Journal of Science and Technology, 32, pp. 315-319, May-June 2010. 74 MODELING OF FERROELECTRIC HYSTERESIS AREA OF HARD LEAD ZIRCONATE TITANATE CERAMICS: ARTIFICIAL NEURAL NETWORK APPROACH Wimalin Laosiritaworn1, Athipong Ngamjarurojana2,3, Rattikorn Yimnirun4, and Yongyut Laosiritaworn2,3,5 1 Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 ThEP Center, CHE, 328 Si Ayutthaya Road, Bangkok 10400, Thailand. 4 School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. 5 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT In this work, the relationship between hysteresis area of hard lead zirconate titanate and external perturbation was modeled using the Artificial Neural Network (ANN). The model developed has the applied electric field parameters and temperature as inputs, and the hysteresis area as an output. Then ANN was trained with experimental data and used to predict hysteresis area of the unseen testing patterns of input. The predicted and the actual data of the testing set were found to agree very well for all considered input parameters. Furthermore, unlike previous power-law investigation where the lowfield data had to be discarded in avoiding non-convergence problem, this work can model the data for the whole range with fine accuracy. This therefore suggests the ANN success in modeling hard ferroelectric hysteresis properties and underlines its superior performance upon typical power-law scaling technique. Published in the Ferroelectrics, 401, pp. 233-238, December 2010. 75 NOVEL FERROCENIC STEROIDAL DRUG DERIVATIVES AND THEIR BIOACTIVITIES Jiradej Manosroi1,2, Kanjana Rueanto1,2, Korawinwich Boonpisuttinant1,2, Worapaka Manosroi, Christophe Biot4, Hiroyuki Akazawa5, Toshihiro Akihisa5, Witchapong Issarangporn6 and Aranya Manosroi1,2 1 Division of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Natural Product Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University, Chiang Mai, 50200, Thailand. 3 Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Université de Lille 1, Uni de Glycobiologie Structurale & Fonctionnelle, CNRS UMR 8576 IFR 147, 59650 Villeneuve d’Ascq Cedex, France. 5 College of Science & Technology, Nihon University, Tokyo, Japan. 6 Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Seven novel ferrocenic derivatives, compounds 1-7, were synthesized from steroidal drugs by Aldol condensation reaction. The derivatives were purified by chromatography, and their structures were determined on the basis of HR-ESI-MS and two-dimensional NMR spectroscopy. The purity of all derivatives wasmore than 95%. Compounds 1-5 demonstrated anti-proliferative activity onHeLa cell line by SRB assay more than their parent compounds. All seven derivatives showed antioxidative activities evaluated by DPPH scavenging and metal ion chelating, while their parent compounds gave no activity. Compound 1 indicated the most potent anti-proliferative activity similar to doxorubicin, with the GI50 at 0.223 (0.014 µg/ mL. Compounds 6 and 7 demonstrated similar potent in vivo anti-inflammatory to their parent compounds (prednisolone and hydrocortisone) at 80.99 (13.5 and 68.24 (10.4% edema inhibition, respectively. This study has suggested that the novel compound 1 was the most potential derivative that can be further developed for cancer treatment. 76 Published in the Journal of Medical Chemistry, 53, pp. 3937-3943, May 2010. PHASE TRANSITION BEHAVIOR IN (1Zx)PZT—xBiAlO3 CERAMICS Nattaya Tawichai1, Tawee Tunkasiri1,2, Sukum Eitssayeam1, Kamonpan Pengpat1 and Gobwute Rujijanagul1,3 1 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. 3 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT In this study, a new ceramic with the composition (1-x)Pb(Zr0.52Ti0.48)O3xBiAlO3 was fabricated using the solid state method. Phase transition characteristic and dielectric response were investigated. With increase in the content of BiAlO3, a transformation from the tetragonal to the rhombohedral phase was observed. The addition also enhanced the degree of phase transition diffuseness and produced a decrease in the ferroelectricto-paraelectric phase transition temperature. The results suggested that BiAlO3 has a strong effect on the transition behavior of the solid solution. It is proposed that lattice distortion and compositional fluctuation are responsible for these effects. Published in the Phase Transitions, 83, pp. 994-1000, October 2010. 77 RELATIONSHIP BETWEEN SUSCEPTIBILITY TO ANTHRACNOSE DISEASE AND ANTIFUNGAL COMPOUNDS CONTENT IN FRUIT LATEX OF THAI MANGO Wilawan Kumpoun1 and Danai Boonyakiat2 1 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Plant Science and Natural Resources, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Mango has a different degree of susceptibility to Anthracnose disease. In the previous study, it was found that latex contained some antifungal compounds which had an effect to control Anthracnose disease caused by Colletotrichum gloeosporiodes. Eight commercial mango cutivars, namely ‘Nam Dokmai’ ‘Rad’, ‘Kaew’, ‘Keaw Juk’, ‘Keaw Sawaey’, ‘Chok Anan’, ‘Keaw Moragot’ and ‘Mahajanaka’, were studied for susceptibility to Anthracnose disease. The relationship between susceptibility to Anthracnose disease and antifungal compounds content in fruit latex were investigated. Crude extract of latex was separated and tested. It was found that antifungal compounds presented only in water insoluble fraction. Most of antifungal compounds w, found in ‘Kaew’ which was the least susceptibility to Anthracnose disease whereas ‘Nam Dokmai’ had the least antifungal compounds and it showed the most susceptibility to Anthracnose disease. The cultivar showed greater susceptibility’ Anthracnose disease had lower the antifungal compounds fraction content in fruit latex. The amount of antifungal compound in fruit latex in each mango cultivar varied from low to high level was ‘Nam Dokmai’, ‘Rad’, ‘Keaw Moragot’, ‘Mahajanaka’, ‘Keaw Juk’, ‘Keaw Sawaey’, ‘Chok Anan’ and ‘Kaew’, respectively. The result showed that antifungal compounds played an important role in controlling Anthracnose disease. 78 Published in the Acta Horticulturae, 877, pp. 1501-1504, 2010. SEQUENTIAL INJECTION ANALYSIS WITH LAB-AT-VALVE (SI-LAV) FOR THE DETERMINATION OF SOLASODINE IN SOLANUM SPECIES Wisanu Thongchai1, Boonsom Liawruangrath1,3 and Saisunee Liawruangrath2,3 1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Applied Chemistry and Environment Research Unit, Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The development of sequential injection analysis with lab-at-valve (LAV) semiautomated system online liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various Solanum species fruits. The main proposed is semi-automated extractive determination of solasodine using methyl orange as colorimetric reagent. After optimization of the system, sample, reagent and organic solvent were sequentially aspirated into an extraction coil connected to the center of a selection valve, where extraction took place by flow reversal. The aqueous and organic phases were separated in a lab-at-valve unit attracted to one of the ports of the selection valve. The absorption of ion-pair solasodine-methyl orange complex in the organic phase was measured spectrophotometrically at 420 nm. The method performances, including reproducibility, linearity, sensitivity and accuracy, were also evaluated. The proposed method is simple, reproducible and accurate. It was successfully applied to the determination of solasodine in Solanum aculeatissimum Jacq., Solanum violaceum Ortega., Solanum melongena Linn. and Solanum indicum Linn. fruits in Solanaceae family. Results obtained were in good agreement with those obtained by batch wise spectrophotometric method. It is also suitable and useful for determination of solasodine in other medicinal plants. Published in the Talanta, 81, pp. 565-571, April 2010. 79 STORAGE LIFE EXTENSION OF EXPORTED ‘NAM DOKMAI’ MANGO BY REFRIGERATED MODIFIED ATMOSPHERE PACKING Wilawan Kumpoun1 and Jamnong Uthaibutra2 1 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Storage life extension methods are important for sea transportation of exported Thai mangoes. This research was conducted to find out the suitable atmosphere in mango package during cold storage. Mango fruit was packed in seal polyethylene terephthalate plastic box with 7 level of perforated holes (0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 cm2/m2) for modified atmosphere in package at different conditions. The fruit were kept at 5°C for 25, 32 and 39 days and transferred to room temperature (25±5°C). Result showed that, mango fruit packed in package with perforated holes 1.5 and 2.0 cm2/m2 and kept at 5°C for 32 days showed normal fruit without chilling injury symptom and normal ripening after removal to room temperature for 7 days. Mango fruit packed in seal package without hole or with few holes showed fermented symptoms. Fruit packed in seal package with too many holes showed chilling injury symptoms. The suitable package for mango e.g. condition of atmosphere in the package at equilibrium, perforated holes size, fruit size, free space of atmosphere in package, and design of package will be presented and discussed. 80 Published in the Acta Horticulturae, 876, pp. 221-226, 2010. THE DEBYE DIELECTRIC BEHAVIOR OF MIXED NORMAL AND RELAXOR-FERROELECTRICS: MONTE CARLO INVESTIGATION Yongyut Laosiritaworn1,2,3, Kanokwan Kanchiang1,2, Athipong Ngamjarurojana1,2, Rattikorn Yimnirun4, Ruyan Guo5 and Amar S. Bhalla5 1 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ThEP Center, CHE, 328 Si Ayutthaya Road, Bangkok 10400, Thailand. 3 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 4 School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. 5 Department of Electrical and Computer Engineering, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249-1644, USA. ABSTRACT In this work, Monte Carlo simulation was used to investigate the Debye dielectric behavior of mixed normal- and relaxor-ferroelectrics. The results show that, with increasing temperature, the dielectric permittivity of the mixed system sharply increases at low temperatures but slowly decays at high temperatures, while still presenting the dielectric peaks close to the relaxor- and normal-ferroelectrics transition temperatures. Further, it is found that the sharpness, the magnitude, and the frequency dispersion of the dielectric peaks can be finely tuned by adjusting relevant normal-ferroelectric parameters in the model. However, the modified Curie-Weiss law shows that its exponent γ is governed by the inverse-temperature dependence as the exponent is very close to 2 regardless of normal-ferroelectric content. The functionalities of the Debye-type dielectric permittivity are then discussed along with the comparison with experiments. Published in the Ferroelectrics, 401, pp. 239-245, December 2010. 81 TRANSDERMAL ABSORPTION ENHANCEMENT THROUGH RAT SKIN OF GALLIDERMIN LOADED IN NIOSOMES Aranya Manosroi1,2, Penpan Khanrin1,2, Warangkana Lohcharoenkal1,2, Rolf G.Werner3, Friedrich Götz, Worapaka Manosroi4 and Jiradej Manosroi1,2 1 Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University, Chiang Mai 50200, Thailand. 2 Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Boehringer Ingelheim International GmbH, 55216 Ingelheim am Rhein, Germany. 4 Department of Microbial Genetics, Faculty of Biology, University of Tübingen, Tübingen, Germany. 5 Faculty of Medicines, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Gallidermin (Gdm) loaded in anionic niosomes composed of Tween 61/ CHL/DP (1:1:0.05 molar ratio) gave the highest entrapment efficiency (45.06%). This formulation gave antibacterial activity against Propionibacterium acnes and Staphylococcus aureus with the MIC and MBC of 3.75 and 7.5; 7.5 and 15µg/µl, respectively. Gdm loaded in niosomes was more chemically stable than Gdm in aqueous solution of about 1.5 times. Gdmloaded and unloaded in niosomeswere not found in the receiver solution investigated by vertical Franz diffusion cells at 37°C for 6 h. Gdm loaded in niosomes showed higher cumulative amounts in viable epidermis and dermis (VED) of rat skin of about 2 timesmore than unloaded Gdm. Gdm loaded in niosomes and incorporated in gel exhibited the highest cumulative amounts (82.42±9.28 µgcm-2) and µuxes (13.74±1.55 µgcm-2h-1) in stratum corneum (SC) and comparative cumulative amounts (183.16±30.32 µgcm-2) and fluxes (25.74±5.05 µgcm-2h-1) in VED to the unloaded Gdm in corporated in gel. This study has suggested that Gdm loaded in anionic niosomes and incorporated in gel is the superior topical antibacterial formulation because of the high accumulation in the skin with no risk of systemic effect. Published in the International Journal of Pharmaceutics, 392, pp. 304-310, June 2010. 82 WEBCAM CAMERA AS A DETECTOR FOR S SIMPLE LAB-ON-CHIP TIME BASED APPROACH Wasin Wongwilai1,2, Somchai Lapanantnoppakhun1,2, Supara Grudpan3 and Kate Grudpan1,2 1 Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Research Laboratory for Development of Analytical Instrumentation, Center for Instrumention and Process, Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 College of Art, Media and Technology, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Amodification of awebcamcamera for use as a small and lowcost detectorwas demonstratedwith a simple lab-on-chip reactor. Real time continuous monitoring of the reaction zone could be done. Acid-base neutralization with phenolphthalein indicator was used as a model reaction. The fading of pink color of the indicator when the acidic solution diffused into the basic solution zone was recorded as the change of red, blue and green colors (%RBG.) The change was related to acid concentration. A low cost portable semi-automation analysis system was achieved. Published in the Talanta, 81, pp.1137-1141, May 2010. 83 Effects of Some Cytokinins, Auxins and Medium Constituents on In Vitro Propagation of Polystachya sp. Chamchuree Sotthikul1,3, Parichat Choomporn2, Siriwan Kammuen1 and Chuntana Suwanthada1,3 1 Department of Plant Science and Natural Resources, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. 2 H.M.the King’s Initiative Centre for Flower and Fruit Propagation, c/o Chiang Mai University, Thailand. 3 Huai Hong Khrai Royal Development Study Centre, Doi Saket, Chiang Mai 52200, Thailand. ABSTRACT A study on in vitro propagation of a wild orchid, Polystachya sp., was carried out by culturing shoots on modified VW (1949) or modified MS (1962) media. It was found that the plants could grow on both modified media supplemented with activated charcoal, 2 mg/L kinetin, or 2 mg/L BAP. MS media with 0.25 mg/L IBA, 0.5-1.0 mg/L NAA promoted root length of 0.40 cm. Combination of 1.0 mg/L NAA and 1.0 mg/L IBA induced 4.10 roots/shoot. The rooting plants could successfully grow in the greenhouse. Published in Asia-Pacific Journal of Molecular Biology and Biotechnology. Volume 8, Issue 1, 2010. pp. 109-112. 84 ANTIMICROBIAL ACTIVITY OF OLIGOMER AND POLYMER CHITOSAN FROM DIFFERENT SOURCES AGAINST FOOD BORNE PATHOGENIC BACTERIA Titima Sukmark1, Pornchai Rachtanapun2,3 and Chitsiri Rachtanapun1,4 1 Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University Bangkok 10900 Thailand. 2 Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100 Thailand. 3 Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, 50200 Thailand. 4 Center for Advance Studies in Agriculture and Food, KU Institute for Advanced Studied, Kasetsart University, Bangkok 10900 Thailand. ABSTRACT The antibacterial activities of oligomer and polymer chitosan from different sources (shrimp, crab and squid) were examined against foodborne pathogenic bacteria. Sixteen tested foodborne microorganisms comprised of eight Grampositive bacteria—namely, Listeria monocytogenes strains 101, 310, 108, Scott A and V7, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis and eight Gram-negative bacteria—namely, Vibrio parahaemolyticus strains BCC 24339, FS 004, TDH 293, TDH 330 and FS 015, Escherichia coli, Salmonella Weltevreden and Salmonella Typhimurium. The minimum inhibitory concentration (MIC) of the chitosans was analyzed by an agar dilution method and the minimum bactericidal concentration (MBC) was analyzed by a broth dilution method. Polymer chitosan from crab showed the best antilisterial activity while polymer chitosan from squid showed the best efficiency for inhibiting S. aureus, B. cereus and B. subtilis. Oligomer chitosan from squid showed the strongest antibacterial activity on V. parahaemolyticus. However, S. Weltevreden was the most resistant to the chitosan groups (MIC = 0.15%v/v). In addition, chitosan with a degree of deacetylation (DD) 98.01% had the strongest antimicrobial activity against the growth of L. monocytogenes and B. cereus. The antibacterial activity against B. cereus was enhanced with increasing DD. Therefore, the antimicrobial activity of chitosan depends on the target microorganism, chitosan source, molecular size and DD. These observations suggested that the source, DD and molecular size of chitosan must be chosen selectively to control the target foodborne pathogens. Published in the Proceeding of 48th Kasetsart University Annual Conference, February 3-5, 2010, Bangkok, Thailand, 27-33. 85 APPROACH FOR LOGOS DESIGN USING MATRIX ANALYSIS TABLE CASE STUDY: LOGOS DESIGN FOR HOTEL IN CHIANG MAI PROVINCE Ponchai Rachtanapun1 and Darin Srisawat2 1 Department of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. 2 Media Arts and Design, The Graduate School (Special Program), Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The approach for logo design using matrix analysis table case study: Logos design for hotels in Chiang Mai Province was studied. The design composition of logo and logo style perception was analyzed by matrix analysis table to improve efficiency of logo design method. It was found that Thai and foreign tourist in Chiang Mai prefer 5 art compositions as following: local culture, distortion, shadow and perspective, motion and plus instrument picture about service, respectively. In additions, the tourists prefer cool color tone and ancient type letter for hotel’s logo. Published in Proceeding of 48th Kasetsart University Annual Conference, February 3-5, 2010, Bangkok, Thailand, 144-147. 86 CHARACTERIZATION OF CARBOXYMETHYL CELLULOSE FILM FROM Mimosa Pigra Pornchai Rachatnapun1 and Nithiya Rattanapanone2 1 Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand 2 Division of Food Science and Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT The Mimosa pigra peel was sun-dried for 2 days and then grounded. The grounded peel was boiled with sodium hydroxide (NaOH) 30%w/v at 100°C for 3 h. The cellulose powder was synthesized to be carboxymethyl cellulose by the reaction of different NaOH concentrations (30%, 40%, 50% and 60%w/v) and monochloro acetic acid in isopropyl alcohol. Effects of various NaOH concentrations on thermal, mechanical properties and water vapor permeability property as well as morphology of cellulose and carboxymethyl cellulose (CMCm) was investigated. The melting point of samples decreased as %NaOH increased. The increase of NaOH concentration (30-50%) resulted in increasing mechanical properties. However, the level of NaOH concentration was 60%, mechanical properties of CMCm films decreased. The result indicated that the highest mechanical properties were provided on 50% NaOH-synthesized CMC film. The WVP of CMC films seemed to be not affected by NaOH concentration. Morphology of cellulose from and CMCm powder was characterized by scanning electron microscopy. The surface roughness of CMCm powder with various NaOH concentrations was not different. In addition, CMCm films were determined the effect of glycerol as a plasticizer on mechanical properties. Increasing amount of glycerol showed an increase elongation at break but decrease tensile strength. Published in the Young Researcher and the Senior Researcher Conference, The Thailand Research Fund, 2010. 87 DEVELOPMENT OF CHITOSAN FILM INCORPORATING GARLIC OIL AND POTASSIUM SORBATE AS ANTIMICRIBIAL AGENTS FOR GARLIC BREAD Jurmkwan Sangsuwan, Pornchai Rachtanapun, Kannanuch Srikok and Jittapa Duangsawat Department of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT The property improvement of chitosan film by incorporating garlic oil or potassium sorbate as antimicrobial agents and application for garlic bread was studied. It was found that chitosan film incorporating garlic oil could extend the shelf life of garlic bread upto 23 days, compared with garlic bread without wrapping which has only 7 days shelf life. While film containing potassium sorbate could extend the shelf life of garlic bread upto 13 days. Both films are acceptable in terms of overall acceptance. Published in Thailand Research Expo, Central World, Bangkok, Thailand, August 26-30, 2010. 88 DIFFERENCES OF SOME NUTRIRTIONAL AND PHYSICAL PROPERTIES of NORTHERN THAI-STYLE FERMENTED SOYBEANS (THUA-NAO) DRIED BY THREE DIFFERENCT METHODS Punpong Lekhakula1, Pichaya Boonprasom2, Pornchai Rachtanapun3 and Panuwan Chantawannakul1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Division of Food Engineering, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. 3 Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. 2 ABSTRACT Effect of three different drying processes (sun-drying, hot-air drying and solar drying) on some nutritional and physical properties of Thua-nao, an indigenous fermented soybean in Northern of Thailand was investigated. Full factorial design of three different drying processes was performed using 2, 4 and 6 hours of drying time. It was found that solar drying and hot-air drying was the most significant method that could reduce the aw and retained more protein content in dried Thua-nao than sun-drying. Hot-air drying could lower the pH of dried Thua-nao drastically. However, the retention of vitamin B12 content, the changes of moisture content and color parameters color parameters of CIE L* a* b* system were not significantly affected by the three drying methods. Thua-nao dried from solar drying method for 6 hours exhibited the highest protein content of 591.82 mg/g dry weight and the lowest aw of 0.74. Solar drying method could be an alternative way to preserve the fermented soybean and prolong shelf-life of dried Thua-nao product with low operating cost in the future. Published in The Proceeding of Food Innovation Asia conference, Bangkok, Thailand, June 17-18, 2010. 89 EFFECT OF AMOUNT OF BLENDED STARCH ADHESIVES AND PARTICLE SIZES FROM TAMARIND BARK ON PROPERTIES OF PARTICLEBOARD Pornchai Rachtanapun, Karnoksak Loiloes and Dumrong Jaikeankea Department of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand ABSTRACT Effect of amount of adhesives (20, 25 and 30g/100 ml) and particle size (7, 12 and 20 mesh) on mechanical properties (modulus of rupture) and physical properties (density, water absorption and thickness swelling) of particleboard from tamarind bark using adhesive from four starches (tapioca starch, mung bean starch, arrowroot starch and sticky rice starch) was investigated. Tamarind bark particle and adhesive was mixed and compressed at 160°C and 1,500 psi for 8 minutes. As amount of adhesive content in particle board increased, density and modulus of rupture of particleboard increased, but water absorption of particleboard decreased. Modulus of rupture, density and water absorption increased as decreasing the particle size. The particle size of 20 mesh at 30g/100 ml adhesive provided the best properties. Published in Proceeding of 48th Kasetsart University Annual Conference, February 3-5, 2010, Bangkok, Thailand, 117-123. 90 EFFECT OF ANTIOXIDANT CONCENTRATION ON WATER VAPOR TRANSMISSION RATE AND SORPTION ISOTHERM OF CASSAVA STARCH-CARBOXYMETHYL CELLULOSE (CMC) FILMS Wirongrong Tongdeesoontorn1, Lisa J. Mauer2, Sasitorn Wongruong3 and Pornchai Rachtanapun4 1 Department of Biotechnology, Graduate School, Chiang Mai University, Chiang Mai, Thailand. Department of Food Science, Purdue University, IN, USA. 3 Division of Biotechnology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, Thailand. 4 Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, Thailand. 2 ABSTRACT The moisture sorption isotherm and water vapor transmission rate (WVTR) of cassava starch-carboxymethyl cellulose (CMC) films with various antioxidants (quercetin and Tertiary buthyl hydroquinone, TBHQ) contents were studied. Moisture sorption isotherm of cassava starch-CMC films were investigated at various relative humidities (0, 11, 33, 54, 75 and 95 %RH) at 25±1°C. The equilibrium moisture content of films dramatically soared above aw = 0.54. Cassava starch-CMC film with quercetin and TBHQ gave higher moisture sorption than cassava starch-CMC film without antioxidant. BET model was found to be the best-fit model for cassava starch-CMC films both with and without antioxidants. Water vapor transmission rate (WVTR) of film samples was performed at 33% and 54% relative humidity (RH) at 25±1°C. WVTR of cassava starch-CMC films increased with addition of antioxidants. WVTR of cassava starch-CMC films containing antioxidant at 54% RH were higher than WVTR of films at 33% RH. Published in International Conference and Exhibition InnoBioplast 2010, September 9-11, 2010, Bangkok, Thailand. 91 EFFECT OF NaOH CONCENTRATION ON SORPTION ISOTHERM OF CARBOXYMETHYL RICE STARCH FILMS AND PREDITION MODELS Pornchai Rachtanapun1 and Wirongrong Tongdeesoontorn2 1 Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University Chiang Mai, Thailand. 2 Department of Biotechnology, Graduate School, Chiang Mai University, Chiang Mai, Thailand. ABSTRACT The effect of sodium hydroxide (NaOH) concentration on the sorption isotherm of carboxymethyl rice starch (CMSr) films was investigated. The knowledge of sorption isotherms is also important for predicting moisture sorption properties of films via empirical models. The moisture sorption isotherm of CMSr films synthesized with various NaOH concentrations (0, 10, 20, 30 and 40 % w/v) were studied at various relative humidities (16, 35, 55 and 76.3% RH), at 25±1°C. The equilibrium moisture content of the films dramatically increased above aw = 0.6. Guggenheim-Anderson-de Boer (GAB), Brunauer-Emmett-Teller (BET) and Oswin sorption models were fitted to the experimental data. The results showed that increasing NaOH concentration caused an increase in the monolayer water content (M0) of films. The GAB model was found to be the best-fit model for CMSr films at aw 0.16-0.76, 25±1°C. Published in The 1st National Rice Research Conference “Moving Rice Research Towards Innovation” Kasetsart University, Bangkhan, Bangkok, Thailand.December 14-16, 2010. 92 EFFECT OF PLASMA TREATMENT ON HYDROPHOBICITY AND BARRIER PROPERTIES OF POLYLACTIC ACID Chanokporn Chaiwong1,2, Pornchai Rachtanapun3, Panchat Wongchaiya1, Rafael Auras4 and Dheerawan Boonyawan1,2 1 Plasma and Beam Physics Research Facility, Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ThEP Centre, 328 Sri Ayuddhaya Rd., Bangkok 14000, Thailand. 3 Department of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand. 4 School of Packaging, Michigan State University, MI, United States, USA. ABSTRACT In this work, we investigated the influence of SF6 plasma generated by an inductively coupled discharge on the hydrophobicity and barrier properties of poly (lactic acid), PLA. It was found that SF6 plasma enhanced the hydrophobicity of PLA as observed in the increase of the water contact angle. Water absorption time of the plasma-treated PLA was two-fold increased. However, the plasma treatment did not have significant influence on water vapor permeability of PLA. The differential scanning calorimetry results revealed that the bulk structure which controls transportation property of PLA remained unchanged after plasma treatment. Atomic force microscopy showed topographical modification after plasma treatment. Attenuated total reflectance Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy showed the bounding of fluorines in the PLA structure, which leads to the increase in hydrophobicity of PLA. Published in Surface and Coatings Technology, 204, 2933-2939. 93 EFFECT OF SULPHUR HEXAFLUORIDE (SF6) PLASMA ON HYDROXPHOBICITY OF METHYL CELLULOSE FILM Pornchai Rachtanapun1,2, Panchat Wongchaiya1 and Dheerawan Boonyawan3,4 1 Department of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Posthavest Research Center, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Chiang Mai, and ThEP Center, CHE, 328 Si Ayutthaya Rd., Bangkok 10400, Thailand. ABSTRACT Effect of plasma treatments (power, pressure and treatment time) on hydrophobicity (contact angle and water absorption time) and sorption isotherm of methylcellulose (MC) film using sulphur hexafluoride (SF6) were investigated. MC film specimens were precondition in desiccators containing silica gel at 25±2°C for 48 h. MC film specimens were plasma treated by an inductivity coupled plasma discharged (ICP) at 13.56 MHz with operating powers (25-75 W) and gas pressures (20-200 mTorr). The treatment times (10-300 second). Hydrophobicity of treated methylcellulose film outstandingly increased. The best plasma treatment condition was power 50 W and 100 mTorr. The treated MC film had higher contact angle and water absorption time than untreated sample at all operated treatment times; contact angle was increased up to 56-80°. These results were corresponding to sorption isotherm. The treated film was absorbed moisture higher than untreated film. 94 Published in Advanced Materials Research, 93-94, 214-218. EFFECT OF UREA FORMALDEHYDE ON PHYSICAL AND MECHANICAL PROPERTIES OF PARTICLEBOARD FROM COFFEE WASTE Pornchai Rachtanapun, Teerarat Sattayarak and Nisachon Katsamak Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this research is to manufacture particleboard by using the coffee waste as the raw material. The main parameter was investigated namely quantity of urea-formaldehyde (UF) adhesive (11, 14.5, 18 and 25%w/w coffee waste). The coffee waste was blended with the UF, then hot-pressed at 140°C and cooled down to room temperature within 8 minutes. The particleboards were tested to certify their properties with Thai industrial standards (TIS 876-2004). The density, moisture content, modulus of rapture (MOR) and modulus of elasticity (MOE) of the sample increased but water absorption decreased with increasing quantity of the UF adhesive. The sample with UF adhesive above 18% were met to the standards (TIS 876-2004). Published in The Sixth Thailand Materials Science and Technology Conference, Miracle Grand Hotel, Bangkok, Thailand, August 26-26, 2010. 95 FLUORINATED DIAMOND-LIKE CARBON COATING ON POLYLACTIC ACID FILM PREPARED BY PLASMA-ENHANCED CHEMICAL VAPOR DEPOSITION Dheerawan Boonyawan1, Chanokporn Chaiwong1, Somruthai Tunma2 and Pornchai Rachtanapun3 1 Department of Physics and Materials Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Plasma and Beam Physics Research Facility, Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Biodegradable film like Poly(lactic acid), PLA has nowadays come to biomedical and food applications with the key role of antibacterial property. This study introduced F-DLC coatings on PLA film by 13.56 MHz PECVD. Acetylene (C2H2) and carbon tetrafluoride (CF4) gas mixture was utilized for film formation. A 32 µm PLA film was pre-activated by argon plasma to perform C-C firs layer and/or C-F bondings. The deposition rate was found to decrease with increasing CF4 due to reactive fluorine ion over carbon. F-DLC thickness measured by an ellipsometer revealed coating thickness of 150-500 nm and roughness of 10-15 ±2 nm with CF4 concentration reduced from 50 to 10 vol.%. The surface energy was found to reduce with increasing fluorine content. By the colony-counting method, it was found that enriched fluorine film reduced E. coli colonies down to 9%. Others measuring results and discussions will be performed and reported. Published in Twelfth International Conference on Plasma Surface Engineering, September 13-17, 2010, in Garmisch-Partenkirchen, Germany. 96 INNOVATION MANAGEMENT THE NEW APPROACH FOR SMALL MEDIUM ENTERPRISE (SME) Nophadon Luangpirom1 and Pornchai Rachtanapun2 1 Faculty of Management Science, Silpakorn University, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Innovation management has been drastically changed in the past 50 years, especially in concept and practices. This research was the exploratory research aimed to develop the innovative capability model of the researcher and find out the factors that affected the innovative capability of the researcher. The research methodology, utilizing the multiple approaches: literature and documents were analyzed for conceptualization; in-depth interview was conducted with senior managements of the innovation organization; statistical analysis using questionnaire to evaluate the feed back from the general researchers i.e. reliability test, validity test, factor analysis, correlation and multiple regression. The finding from the qualitative research revealed the five innovation management concepts including innovation management as invention management, innovation management as technology management, innovation management as marketing management, innovation management as knowledge management and innovation management as chain-link management which had emphasized innovative resources (financial capital, human capital, physical capital and social capital) and innovation management elements in different ways. However, the finding from quantitative research showed and confirmed that all innovative resources and innovation management elements affected the innovative capability of the SME. The findings from both qualitative research and quantitative research, implied that innovation management is no longer conceived as the discrete event arising from isolated individuals but rather considered as interactive process which involving not only tangible assets: financial capital, human capital, physical capital (infrastructure i.e. research institute, science park etc.) but also intangible assets called social capital. Social capital especially “bridging social capital” plays an important role in enhancing and stimulating the innovative capability of the researchers in exchanging and transferring tacit knowledge which is unexplainable by the previous theory of innovation. The research in this area is scanty and needed to be developed for further progression in innovation management theory and its application for both general innovation management and policy decision making. Published in the 1st National Conference on Entrepreneurship and Innovation, December 2-3, 2010, The Far Eastern University, Chiang Mai, Thailand. 97 MORPHOLOGY, PHYSICAL AND CHEMICAL PROPERTIES OF QUEEN PINEAPPLE FRUIT Quyen Dang Thi Mong1,2, Hai Le Ha1 and Pornchai Rachtanapun1,3 1 Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Division of Biotechnology, Faculty of Science, Chiang Mai 50200, Thailand. 3 Division of Packaging Technology, Faculty of Ago-Industry, Chiang Mai 50200, Thailand. 2 ABSTRACT The commercial harvesting of Queen Pineapple (Ananas comosus cv. Phu lae) fruits was evaluated on morphology (fruit weight, size, fruitlet and shape), physical properties (ripening stages, flesh colour as follow: L*, a*, and b* value), chemical properties (total soluble solids (TSS), titratable acidity (TA), and pH). The results showed that the average of fruit weight was 160.0 g. The fruit width and length were 64.58 mm. and 61.26 mm respectively. The numbers of fruitlet (eyes) were between 37-61 fruitlet/ fruit. The fruit shape was spherical. The ripening stages were 3-4 days. The lightness (L* value), a* and b* value were 64.40, 9.72 and 36.54, respectively. The chemical properties were 12.39% Brix for TSS, 0.63 mg/100 mg for TA, and 3.15 for pH, respectively. Published in the Proceeding of 7th International Pineapple Symposium 2010, Convention Center, Johor Bahru, Johor, Malaysia, July 13-15, 2010. 98 PREDICTION MODELS FOR MOISTURE SORPTION ISOTHERMS OF SOY PROTIEN ISOLATE/CARBOXYMETHYL CHITOSAN BLEND FILMS Pornchai Rachtanapun1,2 and Rungsiri Suriyatem1 1 Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. 2 Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50100, Thailand. ABSTRACT The soy protein isolate/carboxymethyl chitosan (SPI/CMCH) blend films incorporated by glycerol were prepared by solution casting. The effect of SPI and CMCH ratio (100:0, 88:12, 67:33, 50:50, 33:67, 12:88, 0:100) on water sorption isotherm was investigated. The moisture sorption isotherms of SPI/CMCH blend films were determined at various relative humidity (16, 35, 55 and 76% RH), at 25±1°C. The isotherms revealed that equilibrium moisture content (%EMC) of SPI/CMCH blend films increased with the increase of the CMCH content, and water content sharply increased above aw = 0.55. Understanding of sorption isotherms is an importance for prediction of moisture sorption properties of films via moisture sorption empirical models. Guggenheim-Anderson-de Boer (GAB), Brunauer-Emmett-Teller (BET) and Oswin sorption models were tested to fit the experimental data. The r2 of all models were similar in the range of 0.906-0.954. The root mean squares (RMS) of GAB, BET and Oswin were in the range of 52.52-95.95, 38.85-58.30 and 698.54-1557.54, respectively. Therefore, BET model was found to be the betterfitted model for SPI/CMCH blend films at 25±1°C. Published in International Conference on Agriculture and Agro-Industry (ICAAI2010), Food, Health and Trade, 19-20 November 2010, Mea Fah Luang University, Chiang Rai, Thailand. 99 PREDICTION MODELS FOR SHELF LIFE OF PUMPKIN CRACKERS IN DIFFERENT PACKAGES BASED ON ITS MOISTURE CONTENT Pornchai Rachtanapun, Nantaporn Kumsuk, Kasame Thipo and Panicha Lorwatcharasupaporn Division of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. ABSTRACT The effect of moisture content on shelf life of pumpkin crackers in four types of packages [high density polyethylene (HDPE), oriented polypropylene (OPP), polyvinilidine chloride (PVDC) and metallized aluminum pouches] was investigated. Since using a sensory test for shelf life determination is costly, our model was developed to predict this quantity. Samples were placed in different percentages of relative humidity (35.5, 44.5, 65.1, 76.4 and 79%) to study their moisture adsorption behavior. The sorption isotherm curve was used to determine critical moisture content (Mc) and the water vapor transmission rate (WVTR) of the pouches was measured. The standard models (constant, linear, middle point, log and Guggenhein-Anderson-de Boer (GAB) model) were tested for shelf life prediction accuracy. The most suitable model for pumpkin crackers was the middle point model which had a discrepancy between the shelf life of the pumpkin crackers in the HDPE sensory test and the model of less than 8.7%. Published in Chiang Mai Journal of Science, 37(3), 1-11. 100 STUDY OF CARBOXYMETHYL CELLULOSE FROM PAPAYA PEELS AS BINDER IN CERAMICS Pornchai Rachtanapun1, Sukum Eitssayeam2 and Kamonpan Pengpat2 1 Department of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. 2 Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Effects of carboxymethyl cellulose from papaya peel (CMCp) on the properties of ceramics were investigated. Cellulose was extracted from papaya peels by NaOH and then it was modified by chloro acetic acid to obtain carboxymethyl cellulose. The prepared CMCp was applied as a binder in ceramic slip in order to increase its viscosity. Sodium silicate was also added into the slip for providing a dispersion of slip and protecting the sedimentation of clay particles. Various concentrations of CMCp and commercial CMC (CMCc) (0.05, 0.1, 0.15, 0.2 and 0.25% w/w of dry weight of clays) were added into the slip. Effect of CMC on viscosity of the slip and the ceramic density were studied. The relationship between the ceramic properties such as, shrinkage, porosity, casting rate and bending strength (both green strength and fired strength), and CMC concentration was also observed. As increasing both CMCp and CMCc, viscosity increased while density decreased. The prepared ceramics (green ware and fired ware) added with CMCp were found to have higher strength and lower in %shrinkage than that of adding with CMCc. The thickness of the ceramic wall adding CMCc increased with increasing CMCc concentration, and time in contrast to that of CMCp as the thickness decreased with increasing CMCp concentration. However, the ceramics with addition of CMCp possessed higher strength than that of the commercial one. Published in Advanced Materials Research, 93-94, 17-21. 101 SURFACE MODIFICATION AND PERMEABILITY ANALYTICAL STUDIES OF POLYLACTIC ACID INCUCED BY HMDSO-PLASMA Chanokporn Chaiwong1, Pornchai Rachtanapun2, Somrutai Tunma3, Rafael Auras4 and Dheerawan Boonyawan1 1 Department of Physics and Materials Science, Chiang Mai 50200, Thailand. Department of Packaging Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Plasma and Beam Physics Research Facility, Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 School of Packaging, Michigan State University, MI, United States, USA. 2 ABSTRACT Poly (lactic acid), PLA, has been increasingly used in packaging industry due to its biodegradability and attractive mechanical properties. In this work, we investigated the influence of hexamethyldisiloxane (HMDSO) plasma generated by an inductively-coupled discharge on the structural and barrier properties of PLA. It was found that HMDSO plasma enhanced the hydrophobicity of PLA as observed in the increase of the water contact angle. Energy-dispersive X-ray spectroscopy (EDX) showed the incorporation of silicon (Si) on the surface of PLA. Atomic force microscopy (AFM) revealed topographical modification after the plasma treatment. Elemental analysis of the surface using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were performed to affirm the bonding of Si onto the surface structure of PLA. Water vapour permeability of PLA was studied in correlation with surface modification induced by the plasma treatment that could affect the transport properties. Published in Twelfth International Conference on Plasma Surface Engineering, September 13-17, 2010, in Garmisch-Partenkirchen, Germany. 102 A Box-splitting Strategy of Interval Newton Method for Finding All DC Solutions of BJT Circuits Sermsak Uatrongjit Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The interval Newton (IN) method is one of the methods which are capable of finding all solutions of a system of nonlinear equations. Many approaches have been proposed to improve the efficiency of the method when applying to problem of finding all direct current (DC) solutions of nonlinear circuits. The IN method consists of many steps, one of them is the box-splitting. This is usually performed by splitting the box in some appropriately chosen coordinate direction to form two new smaller boxes. In this paper, a simple box-splitting strategy is proposed when the IN method is applied to find all DC solutions of a circuit whose nonlinear elements are only diodes and bipolar junction transistors. The idea is to employ the voltage-current characteristic of diode to find the appropriate splitting point. The proposed technique has been implemented using C-XSC toolbox. Its performance is evaluated by testing with some test nonlinear circuits. The results indicate that the proposed technique can reduce the number of search boxes and improve the computation time. Published in ECTI-Con 2010, May 19-21, 2010. 103 A Combustion Aerosol Generator for Submicron Aerosol Production Artit Yawootti, Panich Intra and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A combustion aerosol generator (CAG) for the production of submicron aerosol particles was developed and experimentally studied in this study. Polydisperse aerosols are generated by a laminar diffusion burner with paraffin fuel in the nominal “pre-sooting” condition. The sample particles from the generator were characterized by the scanning electron microscope (SEM), the energy dispersive spectroscopy (EDS), and the long electrical mobility spectrometer (LEMS) for morphology, chemical composition, and particle number concentration and size distribution, respectively. It was found from the SEM image that a large portion of the particles were non-spherical and were agglomerated. Polydisperse aerosols with particle number concentrations of approximately 1014 particles/m3 were obtained. The average diameter of the generated particles obtained by the EMS is in the range of approximately 10 nm to several hundred nanometers. Published in the Proceeding on 3rd International Conference on Technology and Innovation for Sustainable Development, Nong Khai, Thailand, 2010. 104 A particle swarm optimization for wind energy control problem C. Kongnam1 and S. Nuchprayoon2 1 Department of Electrical Maintenance, Electricity Generating Authority of Thailand, Nonthaburi 11130, Thailand. 2 Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The control problem of a wind turbine involves the determination of rotor speed and tip-speed ratio to maximize power and energy capture from the wind. The problem can be formulated as a nonlinear programming problem with the annual energy generation as the objective function. The wind speed distribution is modeled as the Weibull distribution. The Weibull shape and scale parameters are assigned to be stochastic in response to limited wind data and variability nature of the wind. It is proposed to apply particle swarm optimization to solve for optimum rotor speed under fixed-speed operation and optimum tip-speed ratio under variable-speed operation. The optimum rotor speed varies with the wind speed distribution, while the optimum tip-speed ratio does not depend on the wind speed distribution. It can be concluded from the simulation results that both the wind power and energy are more dependent of the Weibull scale parameter than the Weibull shape parameter. This implies that the wind power and energy are more dependent of the mean wind speed than the speed distribution. Key words: Electric power generation; Nonlinear programming; Particle swarm optimization; Wind energy Published in Renewable Energy Volume 35, Issue 11, November 2010, Pages 2431-2438 doi:10.1016/j.renene.2010.02.020. 105 A STUDY ON THE FEASIBILITY OF ZERO-EMISSIONS AIR TRANSPORT Asit Apornsupavit1 and Matthew Cole2 1 International Program Student, Department of Mechanical Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Hydrogen fuel has good potential due to its high energy content per unit weight and carbon-free waste gases. The use of hydrogen fuel has already been proposed for air transport applications including, Auxiliary Power Units with fuel cells for onboard power, distributed power units for Inter-City aircraft with fuel cell propulsion and Ramjets for hypersonic airliners. However there are many technical, financial and political obstacles that must be overcome to make these concepts a reality. This paper examines the main challenges and potential barriers to the adoption of hydrogen as the main fuel in aviation and evaluates the possible contribution to the global transition from hydrocarbon energy towards the low carbon emissions era. Key words: Zero emissions, Air Transport, Hydrogen power, Fuel cell, Ram Air Turbine, Auxiliary Power Units, Inter-City aircraft, Hypersonic airliners Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.279, Chiang Mai, Thailand, November 2009. 106 A Wide-Band Bandpass Filter Using a Novel Embedded Short-Circuited Stub Resonator D. Singwong1 and N. Siripon2 Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This paper presents a wideband bandpass filter using an embedded shortcircuited stub resonator. The resonator consists of a short-circuited stub placed inside the close-loop ring resonator functioning as the parallel circuits of inductor and capacitor. Therefore, its resonant frequency depends on loop dimension and the length of the stub. From the experiment, the resonator cell provides low return loss, sharp band rejection and the circuit size is much smaller than a quarter of wavelength. Then, the simple bandpass filter was designed at 2 GHz by using these resonators. It is found that the filter gives a fractional bandwidth (FBW) of more than 66% measured at the return loss of 20 dB and the insertion loss of less than 1 dB. Moreover, the measurement results are in good agreement with the simulation results. Index Terms: Bandpass Filter (BPF), Microstrip, Short stub, Embedded ShortCircuited Stub Resonator. Published in Proceedings of Asia-Pacific Microwave Conference 2010. 107 ACOUSTIC-BASED SIGNAL TRANSMISSION FOR MANGOSTEEN INTERNAL QUALITY MEASURES Nattapong Swangmuang1, Kasemsak Uthaichana1, Hideyuki Sawada2 and Nipon Theera-Umpon1 1 Electrical Engineering Department, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Intelligent Mechanical Systems Engineering Department, Kagawa University, Takamatsu, Kagawa 761-0396 Japan. ABSTRACT This research investigates an efficient and non-destructive method based on an acoustic response of a signal, aiming to classify internal condition of mangosteens at low cost. In the research, the experiment of acoustic signal transmission is conducted. Acoustic signals at frequencies between 500 to 2,500 Hz are transmitted through mangosteens of different conditions. The response signals are collected on the other side of the mangosteens and analyzed. Further signal processing techniques, including frequency spectrum response, normalization, first difference, and principle component analysis, are applied in this study. The justification whether this method could supplement other methods in distinguishing mangosteen’s qualities is set forth in this study. In the small set of experimental results conducted in this study, it shows that the low-cost acoustic response technique is promising. Published in the Proceedings on the CMU-KU 3rd Joint Symposium, pp 32-34, Chiang Mai, Thailand, August 2010. 108 An Interior Point Method for WLAV State Estimation of Power System with UPFCs Chawasak Rakpenthai1, Suttichai Premrudeepreechacharn2, Sermsak Uatrongjit2, and Neville R. Watson3 1 Department of Electrical Engineering, School of Engineering, Naresuan University Phayao, Phayao 56000, Thailand. 2 Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, New Zealand. ABSTRACT This paper presents a robust state estimator for power system containing unified power flow controller. The estimation problem is formulated as an optimization problem using weighted least absolute value criteria, with a set of equality and inequality constraints. The interior point optimization method is applied to primal and dual formulation in order to solve this problem. Some details of the algorithm, i.e. initialization, step size selection, are also described. The modified IEEE 14- and 118-bus systems are used to demonstrate the effectiveness of the proposed algorithm. The simulation results indicate that the method yields a good state estimate of the system states for a large scale power system with many UPFCs. Published in International Journal of Electrical Power & Energy Systems, Volume 32, Issue 6, July 2010, Pages 671-677. 109 ANALYSIS OF BATTERY USING MICROCONTROLLER Apichart Chotchuen and Sumpun Chaitep Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This research project aims to design, build and test analysis to find out the most appropriate procedure in the charging battery using microcontroller. The set test for charging battery includes the DC supply, 12V/21.6AH battery and the control set. This research study the impact of time in charging battery by changing the charge voltage at 14V, 14.5V and 15V, the discharge voltage at 9.5V, 9.7V, 9.9V, 10.0V, 10.1V, 10.3V, 10.5V, 10.7V, 10.9V, 11.0V, 11.1V, 11.3V, 11.5V, 11.7V and 11.9V and the charge current 5A, 7A, 10A and 20A respectively. Results from the study found that the charging battery by using 15V charge voltage in 11.9V discharge voltage and charge current 20A take the least time. Whereas the charging battery by using 14V charge voltage in 9.5V discharge voltage and charge current 5A take the most time. Factors affecting to the time in charging the battery, according to the most responsive property is the discharge voltage, the charge voltage and the charge current, respectively. Published in the proceedings of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 110 ANALYSIS OF DIESEL ENGINE PERFORMANCE WITH MAYAOHIN AS A FUEL Vorapoj Pothacharoen1, Kanyaporn Chaiwong1, Tanongkiat Kiatsiriroat2, Kitipong Nakpakdee3 and Natthawud Dussadee4 1 Energy Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Mechanical Engineering, Faculty of Engineering, Rajamangala University of Technology Lanna, Chiang Mai 50200, Thailand. 4 Energy Research Center, Maejo University, Chiang Mai 50210, Thailand. ABSTRACT Mayaohin is one type of oil plant and It’s extracted oil could be used as fuel. In this study, performance tast of a diesel engine with the Mayaohin oil in term of Biodiesel and Emulsion compared with that diesel are carried out. The engine has a capacity of 7.5 kW at 2200 rpm. It could br found that the engine power, the tourqe and the fuel consumtion of the engine for the Bio-diesel and Emulsified oil are close to that for the diesel. For emission analysis, the emulsified oil gives the best solution followed by the diesel and Bio-diesel Key words: Mayaohin, Bio-diesel, Diesel engine, Emulsified oil Published in the proceedings of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 111 Analytical discussions on species extinction in competitive communities due to habitat destruction Radom Pongvuthithuma1 and Chulin Likasirib2 1 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT An improvement of a metapopulation model is developed and used to analyze the behaviors of abundant and extinct species in the system analytically. By approximating the direct effect rate of habitat destructions on individual species using differentiable functions, the model enables us to discuss the equilibrium and stability of the system. Special cases of the habitat destruction functions, constant, piecewise linear, exponential and periodic functions, are discussed analytically and numerically. The conditions for system stability are also given. Key words: Species extinction; Habitat destruction; Habitat model; Equilibrium analysis Published in Ecological Modelling Volume 221, Issue 22, 10 November 2010, Pages 2634-2641. 112 Application of Indirect X-Ray Detector by using Contact Image Sensor couple with Field Programmable Gate Array and Mini-CT Kittinan Noimanee, Suranan Noimanee, Chuchart Pintavirooj and Manas Sangworasil Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract This research is the application of indirect x-ray detector by using contact image sensor (CIS), which use field programmable gate array (FPGA) for generate control signal to contact image sensor. The Mini CT is used for x-ray generate to test the indirect x-ray detector with the standard phantom for receive the resolution of contact image sensor (10 line pair per inch). In addition, this research test vary object for receive the x-ray image such as fork, electronic circuit, bone, etc. The whole system can control by computer, which can display x-ray image that sent from field programmable gate array. In the future this research can be developing the x-ray trigger, which can receive x-ray image automatically. This research will be the prototypes for indirect x-ray detector, which can be develop to the medical instrument. Key word: Indirect X-ray Detector, Contact Image Sensor (CIS), Field Programmable Gate Array (FPGA), Mini CT. Published in the proceedings of the 3rd Joint International Information & Communication Technology, Electronic and Electrical Engineering (JICTEE 2010), Luangeprabang, Lao PDR. December 22-24, 2010. Pp. 20-24. 113 Application of Remote Devices Vital-Signs to be Included Telemedicine Mobile Table on WiMAX Network Suranan Noimanee Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The project aimed to research develop of wireless broadband network for medical applications Medical Center, Prince Maha Chakri Sirindhorn’s, HRH Princess Sirindhorn. Amphor Ongklaruck, Nakhon Nayok Province. This project is integrating other sciences. Biomedical engineering, Telecommunications Engineering. Electronic Engineering. Computing technology. Includes both direct medical and so on. When work on this project after the completion of phase 1, then. Have to focus on medical care, community living around the area radius of the radio wave propagation in the district that spreads to the 2500 MHz range in Thailand by the standard of broadband wireless technology. This project involved 4 Subprojects, namely: Subproject I: Development of Multi Patient Monitoring on WiMAX Technology Around MSMC Subproject II: Medical Information Application for Medical Image Data in MSMC Subproject III:Primary Health Care Mobile Doctor around MSMC The works have been conducted by 23 academic staff of whom 11 are older generations, 8 are younger generations, 2 MS students (2 graduated in this period) and 1 BS student (1 graduated in this period). The resulted in medical service to the community surrounding the medical center and around the Health Center, both ongoing. There are 3 papers also works published in international proceedings, 1 paper in national proceeding various impacts have been produced by the project. Key word: WiMAX, wireless broadband network, HRH Princess Sirindhorn hospital center Published in ECTI E-Magazine Vol.4, Issue 3, July-September 2010. 114 APPLICATIONS OF LIFE CYCLE ASSESSMENT TO SUSTAINABLE IDEA Sate Sampattagul Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. abstract Governments around the world are introducing regulations and co-regulatory schemes that are prompting businesses to take action on becoming more sustainable and implement Life Cycle Assessment: LCA (eg. Europe-End of Life Directives for Vehicles and Electronic Equipment, Japan-Law for the Life Cycle Economy, Australia-National Packaging Covenant and proposals for similar co-regulatory schemes for TVs and Computer. Many people have never heard the term of LCA in which is a relatively new approach that brings together different elements of practices that have been used in businesses around the world for decades. LCA grew out of the application of business efficiency and life cycle. In this paper, we would like to share the learnings from our experiences across all aspects of Life Cycle Assessment for generating new idea of sustainability. Key words: Life Cycle Assessment (LCA) Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.293, Chiang Mai, Thailand, November 2009. 115 Appraisal of Appropriate Combustion Air in a Pulverized Coal-fired Boiler Piriya Thongchiew and Tanongkiat Kiatsiriroat Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This objective of this study is to find out the appropriate combustion air in a pulverized coalfired boiler using lignite as fuel at Mae Moh Power Plant. Since the coal property has a wide range quality, therefore, the existing conventional combustion air control system could not completely response to the unstable combustion condition which causes high ash deformation in the boiler and high energy losses. The study was carried out by collecting the boiler operation data from the real-time plant database and analyzing the coal properties. It was found that the coal components and the coal heating value had a linear relation. The correlation was used to estimate the theoretical air by means of the combustion equations. The results of new combustion air were adjusted in the actual practice to operate the boiler at its designed condition. The final results were taken to create the combustion air control diagram for boiler operators to keep the boiler operate at high reliable performance and high efficiency. Published in the Engineering Journal.CMU.[2010] 17 (1),60-71. 116 ARSENIC, CHROMIUM, AND COPPER LEACHING FROM CCA-TREATED WOOD AND THEIR POTENTIAL IMPACTS ON LANDFILL LEACHATE IN A TROPICAL COUNTRY Somjai Karnchanawong1 and Chuleeporn Veerakajohnsak2 1 Department of Environmental Engineering, Faculty of Engineering/National, Centre of Excellence for Environmental and Hazardous Waste Management (NCE-EHWM), Chiang Mai University, Chiang Mai 50200, Thailand. 2 National Centre ofExcellence for Environmental and Hazardous Waste Management (NCEEHWM), Chulalongkorn University, Bangkok 10400, Thailand. ABSTRACT This study looks into the potential risks of arsenic, chromium, and copper leaching from disposed hardwoods treated with chromated copper arsenate (CCA) in a tropical climate. The Toxicity Characteristic Leaching Procedure (TCLP) and the Waste Extraction Test (WET) were employed to examine new CCA-treated Burseraceae and Keruing woods, weathered CCA-treated teak wood, and ash from new CCA-treated Burseraceae wood. In addition, a total of six lysimeters, measuring 2 m high and 203 mm in diameter were prepared to compare the leachate generated from the wood monofills, construction and demolition (C&D) debris landfills and municipal solid waste (MSW) landfills, containing CCA-treated Burseraceae wood. The TCLP and WET results showed that the CCA-treated Burseraceae wood leached higher metal concentrations (i.e. 9.19-17.70 mg/L, 1.14-5.89 mg/L and 4.83-23.89 mg/L for arsenic, chromium, and copper, respectively) than the CCA-treated Keruing wood (i.e. 1.74-11.34 mg/L, 0.26-3.57 mg/L and 0.82-13.64 mg/L for arsenic, chromium and copper, respectively). Ash from the CCA-treated Burseraceae wood leached significantly higher metal concentrations (i.e. 108.5-116.9 mg/L, 15223862 mg/L and 84.03-114.4 mg/L for arsenic, chromium and copper, respectively), making this type of ash of high concern. The lysimeter study results showed that the MSW lysimeter exhibited higher reducing conditions, more biological activities and more dissolved ions in their leachates than the wood monofill and C&D debris lysimeters. All leachates generated from the lysimeters containing the CCAtreated Burseraceae wood contained significantly higher concentrations of arsenic in comparison to those of the untreated wood: in the range of 0.53-15.7 mg/L. It can be concluded that the disposal of CCA-treated Burseraceae wood in an unlined C&D landfill or a MSW landfill has the potential to contaminate groundwater. Key words: leaching test; CCA-treated hardwood; arsenic; chromium; copper Published in Environmental Technology, Vol. 31, No. 4, 1 April 2010, 381-394. 117 Assessment of Lubricating Oil Degradation in Small Motorcycle Engine Fueled with Gasohol Nakorn Tippayawong and Pongpan Sooksarn Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Assessment of the degradation of lubricating oil was performed on the lubricant which had been used in a small motorcycle engine fueled with gasohol in comparison with that run by normal gasoline. The lubricant properties examined in the assessment were lubricating capacity, viscosity and stability to oxidation. Lubricating capacity was evaluated by accelerated wear test on the Timken tester. Lubricating oils from gasohol-run engine appeared to produce about 10% greater wear than that made in oils from gasoline-run engine. There was no significant difference between the effect of gasohol and gasoline on the viscosity of the used lubricating oils. Moreover, no oxidation products in any used oil samples could be detected. Published in Maejo International Journal of Science & Technology, Vol. 4 n. 2, pp. 201209, 2010. 118 BIO-ETHANOL PRODUCTION FROM POTATO WASTE Thanaporn Ngarmprapasom1 and Sumpun Chaitep2 1 Division of Energy Engineering Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Division of Agricultural Engineering Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Possibility of bio-ethanol production from potato waste was investigated. The optimum process was determined by comparing 2 sizes of potato (Atlantic variety), i.e., sliced potato and mashed potato. These 2 sizes of potatoes were then boiled for 30 minutes before added with α- amylase enzyme and glucoamylase. Two varieties of powder active yeast of the Saccharomyces cerevisea, i.e., Fermivin and V1118 were inoculated and fermented at 25 30 and 35°C for 4 days. The result show that the optimum condition was using mashed potato with Fermivin yeast and fermentation at 30°C after fermentation in fermented tank for 3 days, output product was alcohol content of 7.20% (v/v). Published in the Proceedings of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 119 Biogas Production from Animal Waste Using Hybrid Reactor Aekarin Inpramoon, Rotsukon Jawana, Phruk Aukkarungsri and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Biogas from anaerobic digestion of organic materials is a main source of renewable energy in Greater Mekong Subregion. In this study, a hybrid reactor (an upflow anaerobic sludge blanket, UASB, reactor combined with an anaerobic filter reactor) was used. Wastewater from pig farm at 2% total solid was used as feedstock. Experiments were undertaken in two 40 L reactors to compare biogas yields and methane content. The first reactor was UASB, and the second reactor was hybrid reactor. The hybrid reactor has bioball as supporting media filled up to a third of its reactor height. Hydraulic retention times (HRT) used were two and four days. Upflow feeding velocity was controlled at 0.5 m/hr. It was found that HRT of 4 days gave maximum biogas production rate of 17.5 and 21.5 L/day at an average methane content of 76.2% for the UASB, and the hybrid, respectively. The hybrid reactor appeared to give higher efficiency than the UASB. Published in the Proceeding on GMSTEC 2010: International Conference for a Sustainable Greater Mekong Subregion, Bangkok, Thailand, 2010. 120 Biogas Quality Upgrade by Simultaneous Removal of CO2 and H2S in a Packed Column Reactor N. Tippayawong and P. Thanompongchart Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Biogas from anaerobic digestion of biological wastes is a renewable energy resource. It has been used to provide heat, shaft power and electricity. Typical biogas contains 50-65% methane (CH4), 30-45% carbon dioxide (CO2), moisture and traces of hydrogen sulphide (H2S). Presence of CO2 and H2S in biogas affects engine performance adversely. Reducing CO2 and H2S content will significantly improve quality of biogas. In this work, a method for biogas scrubbing and CH4 enrichment is presented. Chemical absorption of CO2 and H2S by aqueous solutions in a packed column was experimentally investigated. The aqueous solutions employed were sodium hydroxide (NaOH), calcium hydroxide (Ca(OH)2) and mono-ethanolamine (MEA). Liquid solvents were circulated through the column, contacting the biogas in countercurrent flow. Absorption characteristics were examined. Test results revealed that the aqueous solutions used were effective in reacting with CO2 in biogas (over 90% removal efficiency), creating CH4 enriched fuel. H2S was removed to below the detection limit. Absorption capability was transient in nature. Saturation was reached in about 50 min for Ca(OH)2, and 100 min for NaOH and MEA, respectively. With regular replacement or regeneration of used solutions, upgraded biogas can be maintained. This technique proved to be promising in upgrading biogas quality. Published in Energy, Vol. 35 n. 12, pp. 4531-4535, 2010. 121 Chemical and physical properties of rice straw waste and hospital sewage sludge in turned windrow aeration system Apisit Sancom1 and Khajornsak Sopajaree2 1 The Joint Graduate School of Energy and Environment, King Mongkut’s University of Technology, Thonburi, Bangkok 10140, Thailand. 2 Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT In this paper, we present the main goal of optimum quality ratio of cocomposting with rice straw waste and hospital sewage sludge. The experiments were designed by varying the initial C/N ratio and moisture content in the range of 25-45 and 30-70% w/w., respectively. The results clearly showed that the losses of Kjeldahl-N increased when the amounts of sludge in the mixtures were increased. The highest carbon decomposition of 49% occurred in the system at a C/N ratio of 35 and 60% of moisture content, which coincided with high nitrogen content in this treatment. In addition, heavy metals and pathogens had lower concentrations than the maximum allowable for final compost for USEPA standards. Key words: co-composting; rice straw waste; hospital sewage sludge; turned windrow aeration; composting; carbon decomposition; moisture content; nitrogen content; heavy metals; pathogens; USEPA standards. Published in International Journal of Environment and Pollution 2010 - Vol. 42, No.1/2/3 pp. 125-135 DOI: 10.1504/IJEP.2010.034230. 122 COMPARISON OF COOLING SYSTEMS WITH CELLULOSE VERSUS GUNNYSACK USING EXCESS COOL WATER FROM ICE FACTORY Insom Norkaew1 and Sumpun Chaitep2 1 Department of Energy Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mechanical Engineering, Faculty of Enginerring, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This research have purpose to compare capability and property between Cellulose and Gunnysack with exceed water from Ice Factory to control temperature in Greenhouse. The Greenhouse made by 2x4x2 meter Plastic polypropylene. The research experiment conditional were using water inlet at 10, 20°C at 1 m/s air speed through wet media and 5 L/min of mass flow rate. At water inlet 10°C the result found that: Cooling pad system can decrease temperature to 2-3°C at 90.96% humidity but in same condition with Gunnysack can decrease temperature to 1-2°C at 86.67 %. At water inlet 20°C the result found that: Cooling pad system can decrease temperature to 3-4°C at 88.54% humidity but in same condition Gunnysack can decrease temperature to 1-2°C at 84.25%. Published in the proceedings of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 123 Continuous Biodiesel Production from Transesterification of Jatropha Oil with Microwave Heating Poramate Sittisun and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Biodiesel (methyl or ethyl ester) is one of major renewable energy sources in Thailand. Typically, biodiesel is produced by transesterification of vegetable oils in a batch reactor, a process which is discontinuous and has low production yield. This study was about laboratory scale, continuous biodiesel production from jatropha oil by transesterification with microwave as a heat source. Sodium methoxide was used as a catalyst at concentrations between 0.25-1.5 wt % oil, with reaction time in microwave irradiation between 10-40 s and oil to methanol molar ratio of 1:3-1:9, respectively. The biodiesel product was analyzed by a thin layer chromatography technique with mobile phase hexane/ ethyl acetate/acetic acid of 90:10:1 by volume. Preliminary results showed that jatropha oil can be converted to methyl ester (96.1%) within 30 s under oil/methanol molar ratio of 1:6 and 1.0% catalyst. The findings indicated that this method can offer alternative means to produce biodiesel continuously. Published in the Proceeding on GMSTEC 2010: International Conference for a Sustainable Greater Mekong Subregion, Bangkok, Thailand, 2010. 124 Control Strategy for Biogas’s Continuous Stirred Tank Reacto Thanakron Paboug and Chatchawan Chaichana Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This article presented the analysis of temperature reaction in a 1-cubic meter Continuous Stirred Tank Reactor used for biogas fermentation. The examination was divided into 2 cases which are regulation problem caused by disturbance from transferring lower-temperature mass into the tank and out of the tank and tracking problem, based on 3 forms of control, these being: feedback control, feed forward control, and combined feed forward - feedback control. The system was modeled by using matlab program which maintained the temperature in the tank at 50°C through the flow rate of constant hightemperature water that went through a heat exchanger. The results of the regulation problem analysis showed that the combined feed forward - feedback control produced a reaction to disturbance better than the feed forward control did alone, while the feedback control caused the worst reaction. For the tracking DRC 44 problem, the experiment found that the combined feed forward -feedback control and feedback control produced a similar reaction to the change of controlled value, and the feed forward control showed the best reaction for this case. Key words: Biogas, Continuous Stirred Tank Reactor, Control Published in the Proceeding on The 24th Conference of the Mechanical Engineering Network of Thailand, October 20-22, 2010, Ubon Ratchathani. 125 Conversion of Bamboo into Glucose by Dilute Acid and Enzymatic Hydrolysis Nuttida Chanhom and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Lignocellulosic biomass is an important alternative energy source to be utilized for ethanol production. In this work, bamboo (Dendrocalamus asper Backer) was used as biomass feedstock. A full grown bamboo with height over 7 m was used. Pretreatment was carried out with dilute sulfuric acid at concentrations between 0.4-1.6% w/w, and residence time between 45-135 min at stable temperature of 140°C. Prehydrolyzate was later analyzed for total sugars by high performance liquid chromatography. For the conditions considered, maximum xylose and glucose yields were obtained from bamboo at 78.83 and 25.83 mg/ml, respectively. Water insoluble solids obtained were hydrolysed with cellulase (Trichoderma reesei) and β-glucosidase (Novozyme 188) for 72 h. It was found that increasing yield was obtained with increasing acid concentration and residence time. In addition, the effects of pretreatment severity and high consistency enzymatic hydrolysis greatly increased the sugar concentrations after the hydrolysis. Published in the Proceeding on GMSTEC 2010: International Conference for a Sustainable Greater Mekong Subregion, Bangkok, Thailand, 2010. 126 Co-operational Optimization of Two Cascade Hydropower Plants Bounchon Soukchaleun and Thawan Sucharitakul Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT In this research, the co-operational optimization of two cascaded hydropower plants in Lao PDR, Xeset1 and Xeset2, was investigated. For Xeset1, there are 5 units which are 2 units of 3.1 MW and 3 units of 13.1 MW. In case of Xeset2 hydro power plant, there are 2 units of 38 MW each, Xeset1 is located downstream from Xeset2 along the Xeset River. The main constraints of the optimization were the inflow of water into the dam and the vibration of each turbine unit. Since, Xeset 1-2 hydro power plants are the run-of-river power plant, therefore, the inflow of water equals to the outflow with no water storage in the reservoir. For the vibration, the main source of vibration of turbine comes from the cavitations. Therefore, the vibration of each turbine was measured at various water flow rate and power output whil the head of water was kept constant. A computer programming was developed to find the optimum operation of each turbine unit to meet the requirement of the highest power and efficiency at various inflows of water while the vibration does not exceed the limit. The output of this research could be applied to find out the most suitable operating condition of Xeset1 and Xeset2 hydro power plant. Key words: Optimization, Hydraulic turbine, Turbine vibration, Turbine efficiency. Published in the Proceeding on The 24th Conference of the Mechanical Engineering Network of Thailand, October 20-22, 2010, Ubon Ratchathani. 127 Design and Theoretical Investigation of a PM2.5 Impactor for Airborne Particulate Matter Sampling Panich Intra1, Artit Yawootti1, Usanee Vinitketkumnuen2 and Nakorn Tippayawong3 1 College of Integrated Science and Technology, Rajamangala University of Technology Lanna, Chiang Mai 50300, Thailand. 2 Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT PM2.5, called a particles less than 2.5 µm in diameter, suspended in the air have adverse PM2effects on human health, air quality and visibility, as well as processes in various industries. Its level of mass concentration is an important parameter in evaluating the degree of hazard it poses to the atmosphere. In this study, a PM2.5 impactor for airborne particulate matter sampling was designed and theoretical investigated. The PM2.5 impactor design can be described as an assembly of an acceleration nozzle and a flat plate, called an impaction plate. In PM2.5 impactor, particles with sufficient inertia are unable to follow the streamlines and will impact on the impaction plate. Smaller particles will follow the streamlines and not be collected on the impaction plate. Analytical and numerical model were developed to prediction of collection efficiency, fluid flow field and vector, and particle trajectory in the PM2.5 impactor under various design parameters. The modeling results suggest that an optimal operational domain exists for the PM2.5 impactor. Finally, a prototype of the PM2.5 impactor is planned to be constructed and tested, based on the results of this model. Published in the Proceeding on The 24th Conference of the Mechanical Engineering Network of Thailand, October 20-22, 2010, Ubon Ratchathani. 128 DESIGN OF LOW SPEED PERMANENT MAGNET GENERATOR Wirat Nakkrongdee1, Nokorn Thonglek1 and Tanongkiat Kiatsiriroat2 1 Department of Electrical Engineering, Faculty of Engineering, Rajamangala University of Technology Lanna, Chiang Mai, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, Thailand. ABSTRACT This paper describes a design of low speed permanent-magnet generator of which the values of voltage, speed, magnetic force and number of coils in the stator are given. In this study a unit at a speed of 600 rpm, 8 poles with the rating of 32 V 3A was designed and constructed. The value of the calculated voltages is close to that of the measured data with a difference of 2.46% average. The constructed model was tested it efficiency when there is a feed current of 3A at a speed of 600 rpm. The output voltage was 33.2 V and the efficiency was 60.4. Published in the proceedings of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 129 Development and Validation of a Battery Model Useful for Discharging and Charging Power Control and Lifetime Estimation Vivek Agarwal1, Kasemsak Uthaichana2, Raymond A. DeCarlo3 and Lefteri H. Tsoukalas1 1 School of Nuclear Engineering, Purdue University, Indiana, USA. Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 School of Electrical and Computer Engineering, Purdue University, Indiana, USA. 2 ABSTRACT Accurate information on battery state-of-charge, expected battery lifetime, and expected battery cycle life is essential for many practical applications. In this paper, we develop a nonchemically based partially linearized (in battery power) input-output battery model, initially developed for lead-acid batteries in a hybrid electric vehicle. We show that with properly tuned parameter values, the model can be extended to different battery types, such as lithium-ion, nickel-metal hydride, and alkaline. The validation results of the model against measured data in terms of power and efficiency at different temperatures are then presented. The model is incorporated with the recovery effect for accurate lifetime estimation. The obtained lifetime estimation results using the proposed model are similar to the ones predicted by the Rakhmatov and Virudhula battery model on a given set of typical loads at room temperature. A possible incorporation of the cycling effect, which determines the battery cycle life, in terms of the maximum available energy approximated at charge/discharge nominal power level is also suggested. The usage of the proposed model is computationally inexpensive, hence implementable in many applications, such as low-power system design, real-time energy management in distributed sensor network, etc. IEEE Transactions on Energy Conversion, 25 (3), pp. 821-835. This work is supported in part by TRF Research Fund. 130 Development of Drop-on-Demand Printer for Rapid Prototyping Applications Mana Saedan Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT There are several technologies introduced to rapid prototype research field. The drop-on-demand printing is one of those technologies in which it is quite new and promises to give lower owner cost than other technologies. Furthermore, the ability to incorporate varieties of materials attracts researchers across the field. However, the current commercialized machines are still expensive and very limited in term of types of materials usage. In addition, they do not allow the use of materials from other vendors beside their own. This inspired us to develop a drop-on-demand printing machine from scratch. The report discusses the major components developments include the print-head in which it is a key component of the drop-on-demand printing system. Furthermore, the paper opens the idea of material development that can be used in our machine. We hope our development will extend the opportunity for advanced research that requires layer-by-layer such as drug delivery devices, sensors and tissue scaffold. Published in the Proceedings on The 24th Conference of the Mechanical Engineering Network of Thailand, October 20-22, 2010, Ubon Ratchathani. 131 DEVELOPMENT OF DRYING KINETICS EQUATIONS OF BANANA SLICE Jutharat Boonpanya and Siva Achariyaviriya Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This research were to study effect of hot air drying and relative humidity on drying rate and quality of dried sliced banana, and to develop drying kinetic equation of slice banana. The banana(Musaaspientumlinn. Musaceae) was used in this study. The sample size of 25-30 mm. in diameter and cut into 5 mm. thick. Sample were dried in the thin layer dryer. The experiments were carried out at constant air velocity of 2 m/s. ,drying air temperature of 50-70°C and relative humidity of 10-40 %. Two types of drying drying kinetic equations were employed for this study: theoretical model and semi - theoretical model. The effective diffusion coefficient and drying constant were determine by fitting the experimental data to drying kinetic equation using least square method. The quality of product was evaluated in term of color. The L*, a*, b* values of sample were measured after drying. It was found that the quality of product was acceptable at drying temperature of 50-60°C and the kinetic model in term of polynomial equation provided the best fit of the data. For the influence of drying conditions on drying rate of sliced banana, it was found that the drying rates increased as the temperature increased at a constant RH, and decreased as the RH increased at a constant temperature. The effect of RH on the apparent moisture content was found that smaller than that of temperature. Published in the Proceedings of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 132 DEVELOPMENT OF MANGO WOOD-CARVING DRYING SYSTEM USING HEAT EXCHANGER FROM BIOMASS FUEL Chakkrawut Taecho1, Sumpun Chaithep2, Natthawud Dussadee3 and Thanes Chaichara3 1 Energy Engineering, Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, Thailand. 3 Energy Research Center, Maejo University, Nongharn, Sansai, Chiang Mai, Thailand. ABSTRACT The objective of this research was developing of mango wood-carvings drying system using heat exchanger from biomass fuel. There were 2 processes; i.e., measurement thermal efficiency of the existing dry mango wood-carvings plant, comparison to the performance of the designed and constructed another new thermal dry mango woodcarvings plant. All 4 sides of wall and a roof are insulating sheet. This dry mango wood-carvings plant is was used water heat exchanging techniques. Hot air was blown into drying room, and then some portion was be recirculated. The smoke would be trapped before they release to the ambient air. Capacity approximately 1,600 kg. per batch of drying. Measurement of thermal efficiency and exhaust emission data between an existing plant compared to the new one, showed with following improvements; thermal efficiency was increased from 4.36% to 9.73% and carbon emissions was reduced from 4,282.56 ppm. to only 10 ppm (decreased 99.76%). Published in the Proceeding of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 133 Development of Monitoring and Control System Using Distributed Embedded System Via Can Bus Pongpol Prompa and Nipapon Siripon Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract This paper presents the node monitoring and control system using distributed embeeded system and CAN bus. Each node adopts 8-bit microcontroller with built-in CAN controller to function as a node’s controller. The CAN highter layer protocol is developed to communicate the data among nodes with CAN’s baud rate of 500 kbps and to handle node’s applications. The proposed system was tested by the on-line car’s position monitoring system. It is found that the last car’s position is reported in every period of 15-30 s via GPRS network. Furthermore, more applications can be created by connecting the devices with the node-such as temperature, humidity and pressure sensor to be the weather analyzer node. Key words: CAN, Higher Layer Protocol, Distributed Embedded System. Published in the proceeding of The 33rd Electrical Engineering Conference, 1-3 December 2010. Organized by KMITL, CMU, MUT. 134 Dilute Acid Pretreatment of Bamboo for Fermentable Sugar Production Wilaiwan Leenakul and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Biochemical conversion of lignocellulosic biomass to ethanol provides a sustainable energy production system. Bamboo is a fast growing woody grass that has great potential to be used as a domestic feedstock for fuel ethanol production. It consists of about 40% cellulose, and 27% hemicelluloses. In this investigation, bamboo (Dendrocalamus asper) was pretreated with dilute sulfuric acid prior to enzymatic hydrolysis process to produce fermentable sugars. The amount of dry feedstock solid/liquid loading at 10% w/w was pretreated in an autoclave at different temperatures (120, 140°C) with different residence times (30, 60, 90 min) and different sulfuric acid concentrations (0.6, 0.9, 1.2% w/w). Maximum glucose and xylose yields were achieved at 140°C, 1.2% sulfuric acid concentration and 90 min. After enzymatic saccharification with cellulase and β-glucosidase at the same pretreatment conditions, the yields of total reducing sugars were found to be low (56 mg/g). On the other hand, the maximum yields of total reducing sugar (85 mg/g) were obtained for the pretreatment conditions at 120°C, 1.2% sulfuric acid concentration and 60 min. Within these conditions, increasing temperature, residence time and acid concentration led to higher total sugar yields and cellulose conversion rates. Published in Journal of Sustainable Energy & Environment, Vol. 1 n. 3, pp. 117-120, 2010. 135 Domestic wastewater treatment by a constructed wetland system planted with rice S. Kantawanichkul and W. Duangjaisak Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The experiments were conducted in four laboratory scale free water surface constructed wetland units made of concrete with the dimensions of 1 m wide, 1.5 m long and 0.8 m deep. The paddy field soil was filled to a depth of 0.4 m and rice seedlings (Oryza sativa L.) were transplanted into the constructed wetland unit at a density of 25 plants/m2. The domestic wastewater from Chiang Mai University was applied into each unit with two different modes to evaluate suitable conditions for wastewater treatment and rice yield. In the first experiment, the wastewater was fed intermittently (7 hours/day) with a hydraulic loading rate of 2, 4, 6 and 8 cm/day. The maximum removal efficiencies for COD, BOD, TKN and SS were only 49.1, 58.7, 64.0 and 59.4% due to short hydraulic retention time for biodegradation of organic substances. In the second experiment, the wastewater was inundated to a depth of 15 cm for 10, 15, 20 and 25 days in each unit and then the wastewater was drained out and flooded again. The removal efficiencies in terms of COD, BOD, TKN and SS were increased especially at 25 day retention time and met the national effluent standard except for SS. The study reveals that apart from wastewater treatment, wastewater can be replaced natural water for rice growing in the dry season or rice can be grown throughout the year. Moreover, nutrients in wastewater can be a substitute for chemical fertilizers. Rice grain production was 4,700 Kg and only 6% less than the production from the conventional paddy field. Published in a proceedings on the 12th International Conference on Wetland Systems for Water Pollution Control. pp.23,Venice, Italy, October, 2010. 136 Drop-on-Demand Printer for Micro Ion-Selective Electrode Fabrication Mana Saedan Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Drop-on-demand (DOD) printing technology has been applied across various fields. Flexibility and efficient use of printing materials attracts applications where object accretion layer-by-layer is essential. This paper presents the application of DOD in manufacturing planar ion-selective electrodes (ISE) from all liquid materials. The custom development of key hardware lowers the cost of DOD machine besides enables direct control to each device. The printing materials are specifically formulated to meet functional demands of ISEs while not compromise dispenser capability. Key words: Drop-on-demand, layer-by-layer, ion-selective electrode, photopolymer, and dispenser Published in Proceedings of the 4th Asia International Symposium on Mechatronics (AISM 2010) Copyright © AISM 2010 Organizers ISBN: 978-981-08-7723-1 137 DRYING OF BANANA USING SUPERHEATED STEAM FOLLOWED BY HOT AIR Nathawit Pongpun, Aree Achariyaviriya, Siva Achariyaviriya and Kodwan Namsanguan Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT An innovative two-stage drying concept is presented in this article. The work considered banana drying using SSD/HAD (superheated steam drying followed by hot air drying) both from drying kinetics and dried product quality points of view. The experiments were performed in the two different stages of SSD/HAD using the superheated steam temperatures of 120-160°C in the first stage and then the drying air temperatures of 80°C in the second stage. The final moisture content of banana was about 6% dry basis. The effects of drying medium temperatures on the drying kinetics and quality of dried banana were investigated. The quality of banana was evaluated in terms of its color characteristics and shrinkage. The results showed that SSD/HAD dried banana had much lower degree of shrinkage, better color and higher degree of rehydration when the steam temperature used in the fires-stage SSD increased. From the experiments, the two-stage SSD/HAD at the steam temperature of 160°C and then the air temperature of 80°C provided the suitable results of drying banana. Key words: Color, Hot air, Shrinkage, Superheated steam, Two-stage drying Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.284, Chiang Mai, Thailand, November 2009. 138 Durability of a Small Agricultural Engine on Biogas/Diesel Dual Fuel Operation Nakorn Tippayawong, Anucha Promwungkwa and Prasert Rerkkriangkrai Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Biogas from anaerobic digestion of organic waste is a potential alternative to the partial substitution of petroleum derived fuels because it is from renewable resources that are widely available. Effect of long term durability tests using biogas/ diesel dual fuel operation on wear characteristics is evaluated and presented in this paper. Steady state tests were performed on a small, single cylinder, naturally aspirated, 4-stroke, direct injection diesel engine at a speed of 1500 rpm, coupled to a generator set to generate electricity for over 3500 hours. Lubricating oil samples were collected during the test run and were subjected to analysis of various wear metal traces present and change in properties. After completion of the endurance test, the engine was dismantled for physical inspection and wear assessment of vital parts. Formation of carbon deposits on in-cylinder surfaces was not found to be problematic. Coking of injector tip did not occur. Surface wear and accumulations of metal debris in crankcase lubricating oil samples were analyzed and found to increase with time, but not in an unusual rate. Properties of used lubricating oils did not alter significantly from their original values. Wear was not significantly different in the test engine fueled with biogas/diesel combination. An overall evaluation of results indicated that the biogas/diesel duel fuel operation could be substituted for diesel fuel in electricity generation and worked satisfactorily under long term engine operation without any major troubles. Published in Department of Mechanical Engineering, Chiang Mai University, 2010. 139 EFFECT OF BOUND NUMBER ON HEAT TRANSFER OF THERMOSYPHON HEAT PIPE UNDER SOUND WAVE Sompon Wongtom1 and Tanongkiat Kiatsiriroat2 1 Department of Mechanical Engineering, Faculty of Engineering, Rajamangala University of Technology Lanna, Chiang Mai, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, Thailand. ABSTRACT Boiling phenomenon of working fluid in thermosyphon heating under sound wave has been caried out.The study has considered the effect of working fluid and inlet water at the evaporator section on heat transfer rate. In the experiment, the heat transfer medium of the thermosyphon is water. The heat pipe is copper tube with 22 mm inner diameter and 1 m length.The evaporator section, the adiabatic section and the condensing section are 0.45, 0.10 and 0.45 m respectively. The working fluid in the heat pipe is ethanol or R123 with a volume of 60%, 70%, 80% of the evaporator. Cold water 25°C and hot water at 50-90°C are used to transfer heat at the heat pipe which is oriented 15, 20, and 25 degree from horizontal plane. A sound wave generator is attached at bottom of the evaporator and it generates wave at 70, 80, 90, and 100 Hz. The input power is 110 W. It could be found that the sound wave could enhance heat transfer, at 15 degree inclination, with hot water temperature at 80°C , sound wave at 90 Hz, filling ratio 80% of evaporator length and R123 as working fluid, the heat transfer rate could be enhanced 26%. Key words: Heat Transfer Rate, Heat Transfer Coefficient, Sound Wave, Working Fluid Published in the Proceeding of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachubkirikhan, 2010. 140 Effect of Needle Cone Angle and Air Flow Rate on Electrostatic Discharge Characteristics of a Corona-needle Ionizer Panich Intra and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT In this study, the corona-needle ionizer was designed, constructed, and characterized. Experimental characterizations of the electrostatic discharge in terms of current-voltage relationships of the corona ionizer, including the effects of discharge electrode cone angle and air flow rate were presented. It was found that the charging current and ion concentration in the charging zone increased monotonically with corona voltage. Conversely, discharge currents decreased with increasing angle of the needle cone. The negative corona was found to have higher current than the positive corona. At higher air flow rates, the ion current and concentration were found to be relatively high for the same corona voltage. The effect of air flow rate was more pronounced than the corona voltage. It was also shown that the ion penetration through the ionizer decreased with increasing corona voltage, and increased with increasing air flow rate. The highest ion penetration through the ionizer of the 10° needle cone angle was found to be about 95.1 and 7.8% for positive and negative coronas, respectively. The highest ion penetration for the needle cone angle of 20° was found to be 46.3 and 4.7% for positive and negative coronas, respectively. Published in Journal of Electrostatics, Vol. 68 n. 3, pp. 254-260, 2010. 141 Effects of Aspect Ratios and Number of Meandering Turns on Performance Limit of an Inclined Closed-Loop Oscillating Heat Pipe N. Panyoyai, P. Terdtoon and P. Sakulchangsatjatai Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Problem statement: The purpose of this research was to determine the effects of aspect ratios and number of meandering turns on performance limit of an inclined closed-loop oscillating heat pipe. Approach: The Closed-Loop Oscillating Heat Pipe (CLOHP) was made of a long copper capillary tube. The geometrical sizes, which were the variable parameters of this study, were as follows; the internal diameter of 0.66, 1.06 and 2.03 mm, the evaporator section length of 50, 100 and 150 mm, the adiabatic and condenser section length of each set was equaled to the evaporator length and the numbers of meandering turn of 5, 10 and 15 turns. The experiments were conducted by setting the inclination angles at 0-90° adjusted by 10°, there totally were 9 sets and each set was tested with R123 as working fluid. The adiabatic temperature was controlled at 50±5°C. The low-voltage and high-current power transformer was used as the heat source and heat transfer rate was obtained by using calorific method at the condenser section. Results: It was found from the experiments that, in case of the CLOHP with 10 turns and the internal diameter of 2.03 mm, the maximum heat flux increases from 10-35.5 kW m-2 when the aspect ratio decreases from 151.5-49.26 and the highest maximum heat flux occurs at inclination angle about 70-90°. Conclusion: The result indicated that the aspect ratio, the ratio of evaporator length by internal diameter and number of meandering turns significantly affect the maximum heat flux and inclination angle. Key words: Inclination, closed-loop oscillating heat pipe, performance limit. Published in Energy Research Journal 1 (2): 91-95, 2010. 142 EFFECTS OF LIP THICKNESS ON ALVEOLAR BONE GRAFT OUTCOMES IN PATIENTS WITH COMPLETE UNILATERAL CLEFT LIP AND PALATE : FINITE ELEMENT ANALYSIS Siraseth Mongkolupatham1, Thongchai Fongsamootr2 and Marasri Chaiworawitkul1 1 Department of Orthodontics and Pediatric Dentistry, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Cleft lip and/or palate are the most common craniofacial anomalies. One step in the treatment protocol is alveolar bone grafting in cleft area. Bone graft resorption always occurs after bone grafting procedure. Objective is to define the effects of lip thickness on alveolar bone graft outcomes in patients with complete unilateral cleft lip and palate. Topographic occlusal radiographs and lateral cephalograms, at 1 month and 1 year after alveolar bone grafting of 10 patients were assessed. Grafted bone heights percentages were measured on the topographic occlusal radiographs whereas lip thicknesses were measured on the lateral cephalograms. A three-dimensional finite element (FE) model of the maxilla was created to investigate the stress affected from the upper lip. Clinical results shown the statistically significant correlation between lip thickness and postsurgical alveolar bone graft height : the less lip thickness, the less alveolar bone graft height remaining. FE analysis results indicated stress-distribution patterns stresses of the maxilla were influenced by lip pressure. These concluded Lip thickness is a factor affecting alveolar bone graft outcome. Key words: lip thickness, alveolar bone graft, cleft lip and palate Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.171, Chiang Mai, Thailand, November 2009. 143 Effects of Reaction Forces at Clamped Edge on Deflection and Stress of Thin Plate Jirasak Panya and Thongchai Fongsamootr Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The objective of this research is to study the effects of reaction forces of clamped edges on the deflection and stress distribution in the thin plate. The deflection and stress equations that include the effect of the reaction forces of the clamped edges, were created. The calculation results of deflection and stress of the thin plate were compared with the results calculated by Lévy - Nádai method. In both cases the boundary conditions of the thin plate were two clamped edges and other two was simply supported edges. Two parameters considered here were aspect ratio (b/a) and applied forces. 14 aspect ratios are studies. The applied forces were varied for 5 values. The results showed that the analyses deflection result were less than the results from Lévy - Nádai in every aspect ratio. And the stress calculated by the new equation were more than the results from Lévy - Nádai for the cases that the aspect ratio was less than 0.8 with 6.946% maximum difference. Otherwise, the calculated results will be less than the Lévy - Nádai results and were constant after aspect ratio greater than 2.5. Finally, the results showed that with increases of applied forces, the deflection of the thin plate would be increases. Published in the Engineering Journal.CMU.[2010] 17 (2),29-40. 144 Effects of Seeding on Performance of Household Organic Waste Composting Using Passive Aeration Siriwan Nissaikla, Somsiri Sattayapanich, Tinnapat Mongkontep and Somjai Karnchanawong Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The objective of this study was to study the effects of seeding, i.e. LDD.1, EM, and mature compost on performance of household organic waste composting using 200 liter passive aeration composting bin. Food wastes and dry leaves were added to each bin once a day for 60 days. The temperature in each bin was recorded daily. A sample of compost from each bin was analyzed for C, N and a germination index were determined once a week, numbers of bacteria fungi actinomycetes were determined twice a month until the 90th day of the composting. It was found that the seeding enhanced the degradation of the organic matter. The composts in un-seeding bin and seeded bin were stable at 88th day and 67th-74th day, respectively. The lowest volatile solids was achieved within a shortest period in the bin with addition of 5% compost. It took about 67 days for composting of waste in this bin with 71.2% decreasing of mass and contained 88.0% proportion of the size smaller than 12.5 mm. Key words: Household organic waste, passive aeration bin, seeding, microorganisms Published in Thai Environmental Engineering Journal, 24(3): pp. 99-109 (2010). 145 EFFECTS OF TEMPERATURE, VELOCITY AND RELATIVE HUMIDITY OF HOT AIR ON SLICED BANANA DRYING Nattawut Kulsatien and Aree Achariyaviriya Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand Abstract The objectives of this research were to study the effects of temperature, velocity and relative humidity of hot air on sliced banana drying and to develop drying kinetic equation of sliced banana. The sample was half ripe peeled banana with 30-35 mm. in diameter and the initial moisture content of 200-220% db., and size of 3 millimeter thickness. The experiments were carried out in an air conditioning dryer at the drying air temperature in a range of 60-100°C, relative humidity in a range of 2.1-26% and air velocity in a range of 0.5-2 m/s. The effective diffusion coefficient was determined by fitting the experimental data to theoretical drying kinetic equation for an infinite slab using least square method. It was found that the effective diffusion coefficient increased and drying time decreased with increasing air temperature and air velocity and with decreasing relative humidity. Also, it was found that model 2, which is in Arrhenius form with linear and exponential equation, provided the best to fit of the data. Published in the Proceedings of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipments and Process. Prachuabkirikhan, 2010. 146 ELECTRIC CYLINDER ENGINE Jirawat Chiatrakul, Viboon Changrue and Sumpun Chaitep Division of Agricultural Engineering, Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT The objective of this study was to propose a new technique of machine able to generate power like the internal combustion engine. This machine was designed using solenoid to produce electromagnetic as an energy resource. Solenoid was installed in the middle of cylinder. It would pull the piston from bottom dead position to center of cylinder. Then the electricity was cut off while piston still keep moving to top dead position and return to bottom dead position by inertia force. The electromagnetic was energized by a limited switch to control magnetic contactor process with power supply of alternating current, AC and the magnetic contactor, was controlled by limited switch, providing DC current to the solenoid (direct current, DC). Testing was conducted by varying voltage from 20, 30, 40, 50, 60, 70, 80 and 90 volt DC. The electric current, shaft speed and torque of the electric cylinder engine was recorded. The result revealed that this machine provided complete cycle moving when voltage reach 50 volt DC while electric current in solenoid was 4.7 ampere and provided shaft speed was 159.5 rpm. At 60 volt DC, electric current in solenoid was 5.8 ampere and shaft speed was 235.1 rpm. At 70 volt DC electric current in solenoid was 7.0 ampere and shaft speed was 270.8 rpm. At 80 volt DC electric current in solenoid was 7.8 ampere and shaft speed was 313.3 rpm and at 90 volt DC electric current in solenoid was 8.8 ampere and shaft speed was 352.9 rpm. highest efficiency rate was 2.98%. and output torque at the rotating crankshaft at 0.62 N-m. Key words: Electromagnetic, engine Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.280, Chiang Mai, Thailand, November 2009. 147 ENHANCMENT OF ELECTROSTATIC PRECIPITATOR FOR SUBMICRON PARTICLE COLLECTION USING NON-THERMAL PLASMA TECHNIQUE Nakhorn Thonglek1 and Tanonkiat Kiatsiriroat2 1 Department of Energy Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50202, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai 50202, Thailand. ABSTRACT Removing submicron particles from air is very important because most of them, with a size smaller than 1 .m have a detrimental effect on human health. High particle collection efficiency in terms of particle weight/volume is well achieved by a conventional electrostatic precipitator (ESP). However, the collection efficiencies in terms of number density for the submicron particles by conventional ESP are still relatively low. Therefore, it is necessary to improve the collection efficiency for submicron particles. By forming large particles, plasma agglomeration of submicron particles is presented as a promising process for enhancing the efficiency of the current electrostatic precipitation for small size particle removal. This research is to study the effect of flow rate of combustion gas, pulse peak voltage and pulse frequency on the plasma agglomeration of carbon particles in nanometer size range. For the agglomeration test, the experimental consists of a wire-plate electrostatic precipitator with a saw-tooth electrode. For the ESP under pulse corona energization, the experimental results show that the particle reduction efficiency curve has the highest reduction efficiency for particles with a diameter near 200 nm. The particle reduction efficiency increases with increasing pulse peak voltage and pulse frequency, but decreases if the gas velocity is high. Key words: plasma technique Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.401, Chiang Mai, Thailand, November 2009. 148 EVALUATION OF ELECTRICITY GENERATION POTENTIAL FROMWIND TURBINE FARM AT MHAE ROYAL PROJECT SAMOENG DISTRICT, CHIANG MAI PROVINCE,THAILAND Wasan Pinatae1, Tanongkiat Kiatsiriroat1, Natthawud Dussadee2 and Kittikorn Sasujit2 1 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Energy Research Center, Maejo University, Nongharn, Sansai, Chiang Mai 50210, Thailand. ABSTRACT Potential on electricity generation from wind turbine with the measured wind data at Mae Hae Royal Project has been considered. The wind speeds at altitudes of 20, 40 and 80 m above ground and wind direction every 10 minutes for a period of 1 year (June 2008- May 2009) were measured and recorded. The recorded data at the altitudes of 80 m were used to analyze the wind climate by WAsP 9.1 computer software and generate Weibull distribution with parameters K and A. A vector map covering the area 10x10 km2 around each meteorological station was produced. The appropriate wind turbine farm scales of 0.3 0.45 0.6 1.0 and 1.3 MW were considered from annual energy production (AEP), the capacity factor (C.F.), the cost of energy (CoE), and the CO2 emission avoidance from the wind power plant installation. The results showed that the size of 0.6 MW were utmost possible due to the highest AEP which was 9.87 GWh/year. The corresponding capacity factor was 37.65%. The CoE was the 2.576 Baht/kWh and the payback period was 6.60 years. Finally, it was found that the 3 MW wind power plant operation 5 units of 0.6 MW wind turbine could avoid CO2 emission about 2,183.10 ton CO2/year. Key word: Wind Energy/ Wind Farm Turbine/power potential Published in the Proceedings of the 9th Conference of Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachubkirikhan, 2010. 149 Evaluation of Energy Absorption Capability of Light Truck’s Front Nudge Bar Paccamon Thammitsakun and Anucha Promwangkwa Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A light truck’s front nudge bar is an optional structure which be chosen by customer satisfier and plays a great role to protect vulnerable car parts. The bar may not design to protect passengers from impact. In order to evaluate the energy absorbing capabilities of front nudge bar during the frontal crash into rigid wall, this study uses non-linear finite element analysis to simulate the crash and to compare the effects of three main factors: bar shape, thickness of bar material and crash velocity. The simulation results showed that crash velocity is the most important factor in energy absorption. Front nudge bars can absorb most of energy if vehicle velocity is less than 18.5 km/h. At higher vehicle speed, percentage of energy absorbing capability decreases. Deformation energies of nudge bars at 30 and 56 km/h are 64.4% and 23.33% of vehicle kinetic energy before impact, respectively. The study of bar shapes and thickness of bar material show less significant to energy absorbing values. Analysis of maximum acceleration during impact shows mainly depends on crash speed. Published in the Engineering Journal.CMU.[2010] 17(3),43-49. 150 Experimental Investigation of an Automotive Air Conditioning System Driven by a Small Biogas Engine Det Damrongsak and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A compact air conditioning module run on biogas for rural use is proposed. The research study is to investigate the use of small biogas engine to drive the automotive vapour-compression air-conditioning system. The engine used is singlecylinder, four-stroke gasoline engine with capacity of 125 cm3 and compression ratio of 11:1. The biogas engine can be used to run the air-conditioning system with acceptable operation over a range of speeds and loads. The modular system can operate at a range of cooling loads above 3.5 kW at high coefficient of performance, with the proper speed ratio between the engine and the compressor. Overall primary energy ratio of the modular refrigeration system driven by the biogas engine was found to be maximum at about 1.0-1.2. The performance of the modular system tends to decrease with an increase in engine speed. Published in Applied Thermal Engineering, Vol. 30 n. 5, pp. 400-405,2010. 151 Finding All DC Solutions of Diode and BJT Circuits by Interval Method and Modified LP-Narrowing Technique Sermsak Uatrongjit Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The method based on interval algorithm is one of techniques to find all direct current operating points of nonlinear circuits. Many approaches have been proposed to enhance the efficiency of the interval method. Amongst them, the linear programming narrowing (LP-narrowing) strategy has been proposed and it shows very promising results. In this paper, a modification of the LP-narrowing technique is proposed. The proposed technique is applied with the interval based method to find all direct current solutions of a circuit whose nonlinear elements are only diodes and bipolar junction transistors. It employs the voltage-current characteristic of diode to contract the size of the box under consideration before applying the interval operator. The proposed technique has been implemented by using C-XSC toolbox and GNU Linear Programming Kit package. Its performance is evaluated by testing with some nonlinear circuits. The results indicate that the proposed technique can reduce the number of search boxes and improve the computation time. Published in IEEJ Transections on Electrical and Electronic Engineering, Vol. 5, No. 6 Nov. 2010, pp.621-626. 152 Gasification of Giant Sensitive Plants in a Fixed Bed Reactor Thanasit Wongsiriamnuay and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A giant sensitive plant (Mimosa pigra L.) is a fast growing and spreadable weed. It infests strongly along the rivers, surrounding large reservoirs, wetland reserves and agricultural fields. Its invasion threatens the production and preservation values of wetlands, and poses a major problem in agricultural areas. To avoid food-fuel dilemma, the weed may be utilized as a biorenewable energy source. In this study, it was used as feedstock for generation of producer gas. Mimosa sample were collected, and air dried. They were subsequently milled, sieved and classified into fractions of uniform particle size between 0.3 and 0.6 mm. Fuel characterization was performed using proximate and ultimate analyses. Gasification of giant sensitive plants was carried out at atmospheric pressure in a laboratoryscale fixed bed reactor to investigate the effect of reactor temperature and catalyst biomass ratio on gas yields and product gas composition. The product gas from thermochemical process was found to contain high CO and H2 which was a useful low heating value gaseous fuel. With an increase in temperature, gas yield was found to increase while char and tar were found to decrease. Increasing catalyst to biomass ratio was found to result in an increase in hydrogen, and a decrease in carbon monoxide. It appeared that the weed can be utilized as a useful renewable fuel. Published in the Proceeding on 23rd International Conference on Efficiency, Cost, Optimization, Simulation and Environmental Impact of Energy Systems, Lausanne, Switzerland 2010. 153 Heat and mass transfer in combined convective and far-infrared drying of fruit leather Somkiat Jaturonglumlert and Tanongkiat Kiatsiriroat Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Combined convective and far-infrared drying is a challenging assignment due to complex relationship between heat and mass transfer. In this paper, heat and mass transfer of fruit leather drying with combination of hot air and far-infrared has been carried out. The heat and the mass transfer coefficients were analyzed by heat-mass analogy. It could be found that the ratio between heat and mass transfer coefficients for the combination technique could not be obtained from the heat-mass analogy classical model and a modification is needed. The modified correlations for predicting ratio of heat and mass transfer coefficients and the heat transfer coefficient in term of heat transfer Nusselt number are developed. The model could fit the experimental data quite well within ±10% deviation. Key words: Heat-mass analogy; Heat and mass transfer coefficient; Far-infrared radiation;Hot air drying; Fruit leather Published in Journal of Food Engineering, Volume 100, Issue 2, September 2010, Pages 254-260. 154 Heat extraction from salinity-gradient solar ponds using heat pipe heat exchangers Sura Tundee1, Pradit Terdtoon1, Phrut Sakulchangsatjatai1, Randeep Singh2 and Aliakbar Akbarzadeh2 1 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Energy Conservation and Renewable Energy Group, School of Aerospace, Mechanical and Manufacturing Engineering, RMIT University,Bundoora East Campus, Bundoora, Victoria 3083, Australia. ABSTRACT This paper presents the results of experimental and theoretical analysis on the heat extraction process from solar pond by using the heat pipe heat exchanger. In order to conduct research work, a small scale experimental solar pond with an area of 7.0 m2 and a depth of 1.5 m was built at Khon Kaen in North-Eastern Thailand (16270N102E). Heat was successfully extracted from the lower convective zone (LCZ) of the solar pond by using a heat pipe heat exchanger made from 60 copper tubes with 21 mm inside diameter and 22 mm outside diameter. The length of the evaporator and condenser section was 800 mm and 200 mm respectively. R134a was used as the heat transfer fluid in the experiment. The theoretical model was formulated for the solar pond heat extraction on the basis of the energy conservation equations and by using the solar radiation data for the above location. Numerical methods were used to solve the modeling equations. In the analysis, the performance of heat exchanger is investigated by varying the velocity of inlet air used to extract heat from the condenser end of the heat pipe heat exchanger (HPHE). Air velocity was found to have a significant influence on the effectiveness of heat pipe heat exchanger. In the present investigation, there was an increase in effectiveness by 43% as the air velocity was decreased from 5 m/s to 1 m/s. The results obtained from the theoretical model showed good agreement with the experimental data. Key words: Solar pond; Thermosyphon; Heat pipe heat exchanger; Heat recovery; Renewable energy Published in Solar Energy, Volume 84, Issue 9, September 2010, Pages 1706-1716. 155 Identification of hyperelastic material parameters using Virtual Fields Method in case of large deformations Nattawit Pomma Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This present paper deals with the identification of constitutive parameters of a hyperelastic law from heterogeneous stress and strain fields using the Virtual Fields Method (VFM). The main idea consists in the exploitation of heterogeneous kinematic fields in order to directly identify all the constitutive parameters from only one test. The main drawback of such an approach is the fact that no direct link between displacement measurement data and unknown parameters is available in the general case. In this paper, the VFM is applied for the first time in the case of large deformations. The approach is developed here for a hyperelastic law in order to test the performances of this method in case of large deformations. Results found in terms of constitutive parameters are in good agreement with usual values of the selected materials. Key words: Virtual Fields Method, large deformations, hyperelastic material, Mooney law Published in the Proceeding on The 24th Conference of the Mechanical Engineering Network of Thailand, October 20-22, 2010, Ubon Ratchathani. 156 Image Restoration Based on a Pair of Noisy and Motion Blurred Images Karn Patanukhom Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A image restoration scheme using a pair of noisy and motion blurred images is proposed. The restoration scheme combines a deconvolution algorithm with a denoising technique. The denoising technique is initially applied to reduce noises. After the blur function has been identified, the residual noise from the denoising process is estimated by using deconvolution scheme. Unlike conventional schemes using the pair of images, the proposed scheme tries to estimate an optimal restoration result in consideration of small noises in the blurred images. An iterative deconvolution algorithm with optimal regional stopping rules is proposed. The images are decomposed into several regions based on a variance of the residual noise. Optimal regional terminating points are independently assigned in order to minimize a restoration error in each region. The optimal terminating point can be decided by analyzing a relation between a discrepancy error and the restoration error. The results from simulation are given to demonstrate the performance of the proposed method. Published in the Proceedings of Electrical Engineering/Electronics Computer Telecommunications and Information Technology CECTI-CON), 2010 International Conference on 19-21 May 2010, pp. 693-697, Chiang Mai. 157 In vivo characterization of the aortic wall stress-strain relationship Asawinee Danpinid1,2, Jianwen Luo2, Jonathan Vappou2, Pradit Terdtoon1 and Elisa E. Konofagou2 1 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Biomedical Engineering, Columbia University, New York, NY, USA. ABSTRACT Arterial stiffness has been shown to be a good indicator of arterial wall disease. However, a single parameter is insufficient to describe the complex stress-strain relationship of a multi-component, non-linear tissue such as the aorta. We therefore propose a new approach to measure the stress-strain relationship locally in vivo noninvasively, and present a clinically relevant parameter describing the mechanical interaction between aortic wall constituents. The slope change of the circumferential stress-strain curve was hypothesized to be related to the contribution of elastin and collagen, and was defined as the transition strain (eTh). A two-parallel spring model was employed and three Young’s moduli were accordingly evaluated, i.e., corresponding to the: elastic lamellae (E1), elastin-collagen fibers (E2) and collagen fibers (E3). Our study was performed on normal and Angiotensin II (AngII)-treated mouse abdominal aortas using the aortic pressure after catheterization and the local aortic wall diameters change from a cross-correlation technique on the radio frequency (RF) ultrasound signal at 30 MHz and frame rate of 8 kHz. Using our technique, the transition strain and three Young’s moduli in both normal and pathological aortas were mapped in 2D. The slope change of the circumferential stress-strain curve was first observed in vivo under physiologic conditions. The transition strain was found at a lower strain level in the AngII-treated case, i.e., 0.029±0.006 for the normal and 0.012±0.004 for the AngII-treated aortas. E1, E2 and E3 were equal to 69.7±18.6, 214.5±65.8 and 144.8±55.2 kPa for the normal aortas, and 222.1±114.8, 775.0±586.4 and 552.9±519.1 kPa for the AngII-treated 158 aortas, respectively. This is because of the alteration of structures and content of the wall constituents, the degradation of elastic lamella and collagen formation due to AngII treatment. While such values illustrate the alteration of structure and content of the wall constituents related to AngII treatment, limitations regarding physical assumptions (isotropic, linear elastic) should be kept in mind. The transition strain, however, was shown to be a pressure independent parameter that can be clinically relevant and noninvasively measured using ultrasound-based motion estimation techniques. In conclusion, our novel methodology can assess the stress-strain relationship of the aortic wall locally in vivo and quantify important parameters for the detection and characterization of vascular disease. Published in Ultrasonics, Volume 50, Issue 7, June 2010, Pages 654-665. 159 Incorporating SIFT with Hard C-Means Algorithm Wattanapong Suttapak1, Sansanee Auephanwiriyakul1,2 and Nipon Theera-Umpon2,3 1 Computer Engineering Department, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Biomedical Engineering Center, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Electrical Engineering Department, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The scale invariant feature transform (SIFT) has been used widely as a tool in object recognition. However, when there are several keyframes for one object in the training database, the number of keypoint descriptors for that object might be huge. The matching process of a test keypoint has to be done on all keypoints in the training database, hence, the amount of matching time is huge. Since the keyframes in the training database are from the same object, there must be some keypoints that are similar. In this paper we incorporate SIFT with the Hard C-Means (HCM) algorithm to group keypoint descriptors and then utilize the prototypes in the matching process instead. We implement this algorithm with three data sets, i.e., bottle, MPEG 7 and Thai hand gesture. We found that on the bottle and MPEG 7 test data sets, the algorithm outperform the one with SIFT with much smaller matching computation time. For the Thai hand gesture data set, the correct classification with much less matching times on the test data set from the proposed algorithm is comparable with that of SIFT. Key words: Scale Invariant Feature Transform (SIFT), Hard C-Means (HCM), Object Recognition, Thai Hand Gesture Published in the Proceedings on Computer and Automation Engineering (ICCAE), 2010 The 2nd International Conference on 26-28 Feb. 2010, pp.437-441, Singapore. 160 Investigation of Light Tar Cracking in a Gliding Arc Plasma System Nakorn Tippayawong and Passakorn Inthasan Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Utilization of biomass gasification technology is not widely adopted due largely to tars contained in the product gas that pose serious problems, especially in engine use. In this paper, a laboratory scale plasma system for light tar cracking was developed. The system was based on non-equilibrium plasmas generated from two gliding arc plasma in series. Light tar components were represented by naphthalene in nitrogen flow, generated at constant rates between 130-690 mg/m3. Destruction efficiency and specific energy use were evaluated. It was found that destruction efficiency in the range of 70-95% could be achieved with a simple, single stage reactor. Plasma assisted tar decomposition was found to increase with applied electrical energy. Complete destruction (>99%) of light tars was obtained with the two-stage, gliding arc plasma system with applied electrical energy of about 1.8-2.9 kWh/m3. Published in International Journal of Chemical Reactor Engineering, Vol. 8 (2010). 161 Kinetics and modeling of whole longan with combined infrared and hot air Paradorn Nuthong, Aree Achariyaviriya, Kodkwan Namsanguan and Siva Achariyaviriya Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT The aim of this research was to evaluate the effects of operating variables on the drying behavior of whole longan undergoing a combined infrared and hot air drying process, to determine its kinetic parameters, and to develop drying kinetic models. The single-layer drying experiments were carried out at infrared powers of 300, 500 and 700 W, drying air temperatures of 40, 60 and 80°C, and air velocities of 0.5, 1.0 and 1.5 m/s. The samples were dried until attaining a final moisture content of 0.2 kg water/kg dry solid. The results show that the drying had a short constant rate period followed by a falling rate period in all cases. The drying rate and product temperature were significantly influenced by infrared power, temperature and velocity of ambient air. In the constant rate period, the mass transfer coefficient varied from 3.646 103 to 1.914 102 m/s. It increased with increasing infrared power, but decreased as air velocity and air temperature increased. In the falling rate period, theoretical and semi-empirical drying kinetic equations were used to describe the drying kinetics of the product. It was found that the overall effective diffusion coefficient and drying constant varied from 7.012 1011 to 6.681 1010 m2/s and 0.026 to 0.234 h1, respectively. Both parameters increased with increasing infrared power and air temperature, but decreased with increasing air velocity. Combined regression equations developed to predict the drying kinetic parameters (hD, Deff and k) for all three models gave a fairly good fit. Published in Journal of Food Engineering, Volume 102, Issue 3, February 2011, Pages 233-239. 162 LEACHING OF HEAVY METAL FROM SPENT HOUSEHOLD BATTERIES Pawena Limpiteeprakan1 and Somjai Karnchanawong2 1 Collage of Medicine and Public Health, Ubon Rajathanee University, Ubon Rajathanee 34190, Thailand. 2 Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The objective of this study was aimed to conduct the leaching test from 36 pieces of 4 types of spent batteries, i.e. Zinc-carbon, Alkaline, Ni-Cd and Ni-MH and from 5 sizes, i.e. AA, AAA, C, D and 9V. TCLP was performed on the leaching test. in order to address the concerns associated with the leaching of chemicals from batteries into the landfill leachate. The results showed that types of spent batteries has influenced on concentration of heavy metal in leaching solution. Zinc-carbon batteries and alkaline batteries released high amounts of Mn and Zn at 0.12-55.7 mg./l. and 7-711 mg./l., respectively. As for the rechargeable battery, Ni-Cd, it generated a leaching solution containing a high Cd concentration at 8.48-46.9 mg./l. Only the Ni-MH battery produced amounts that were lower than the regulatory limits for all the analyzed heavy metals. These results indicate that the direct disposal of some type of spent household batteries into a municipal solid waste landfill can increase the heavy metal contents in the landfill leachate. Key words: TCLP leaching test; spent household battery; heavy metals; leachate Published in the Proceedings on the 9th National Environmental Conference, 24-27 March 2010. Ubonratchatani. 163 Life Cycle Assessment of Frozen Okra Production by Parameter Screening Method Supalak Palee, Sate Sampattagul, Natanee Vorayos and Tanongkiat Kiatsiriroat Energy Engineering, Department of Mechanical Engineering, Faculty of Engineer, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Different systems and methodology of Life Cycle Assessment (LCA) has been developed all over the world. The life cycle assessment at SMEs is quite difficult due to complexity and high cost of the LCA process. This problem was addressed on the experts’ experience in the development of Streamlined LCA for SMEs. In this project, the screening life cycle assessment was applied for an agricultural product in Thailand, which is frozen okra. The environmental impact of this product is assessed by the screening LCA and the evaluation method is Environmentally Responsible Product Assessment: ERPA method. The purpose is to identify the principle parts of life cycle that are important to the environmental impact. The system investigated includes 5 life stages; cultivation, manufacture, transportation, product use, and disposal. The environmental impact is classified into 5 environmental stressors, which are material choices, energy use, solid waste, waste water, and air pollution. The emissions are assessed by rating from collected highest impact level of each parameter such as fertilize, chemical use, heavy oil, and emission from energy use. All of the rating values were calculated by simple equation. Finally, the environmental stressor of the whole life cycle stage was showed in a format of target plots for a 25-element matrix. The overall rating (RERP) is 55.93; coolant (ammonia and R-22) and energy use in manufacture stage are found to be hot-spots. Air pollutions especially green house gas from manufacture and transportation stages are found to be a critical parameter. Key words: Environmental stressors, ERPA, Frozen Okra, Life Cycle Assessment, Screening LCA Published in the Proceeding on International Conference the 17th Tri-University, International Joint Seminar and Symposium, 9-13 November 2010, Chiang Mai, Thailand. 164 Mathematical Model of the Optimum Heat Pipe Heat Exchanger for a Condenser of Vapor-Compression Refrigeration Cycle P. Yeunyongkul, P. Sakulchangsatjatai and P. Terdtoon Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Problem statement: This study theoretically investigated applying heat pipe as a heat exchanger in the condenser of vapor compression refrigeration system for sustainable well-being. Splittype air conditioner for residential propose was considered. To reduce pressure drop and recover heat from the condensing process of the refrigeration cycle, this investigation tried to use Closed Loop Oscillating Heat Pipe (CLOHP) instead of the conventional condenser in split-type air conditioner. Approach: The system was single stage with reciprocating compressor which operated at steady state. The refrigerating capacity was 12,500 Btu h-1 and refrigerant was R22. The vapor compression refrigeration system was simulated to determine effect of mass flow rate of refrigerant on various parameters; such as refrigerating capacity, compressor power, heat rejection of condenser and Coefficient of Performance. Results: It was found that, at the normal operating and 3,663 W of the cooling load, mass flow rate of refrigerant, compressor power, heat rejection of condenser and Coefficient of Performance were 0.031 kg sec-1, 1,174 W, 4,837 W and 3.1, respectively. In addition, an increase in evaporating temperature or a decrease in condensing temperature results in increase of refrigerating capacity. CLOHP heat exchanger was simulated to predict optimum sizing on the basis of thermo-economical method. It was found that the optimum sizing of CLOHP heat exchanger with R123 as working fluid were; 0.1 m of evaporator section Length (Le), 0.1 m of condenser section Length (Lc), 2.03 mm of inner Diameter (Di) and 218 turns of number of turn (N). The optimum sizing when water was used as working fluids were 0.1 m of Le, 0.1 m of Lc, 2.03 mm of Di and 176 of N. Finally, the optimum sizing when ethanol was used as working fluids were 0.1 m of Le, 0.1 m of Lc, 2.03 mm of Di and 243 of N. Moreover, net saving of R123, water and ethanol systems at the optimum size were 9,095, 9,117 and 9,082 USD, respectively. Conclusion: The optimum AHE, N, Le, Lc and Di are 0.45 m2, 176 turns, 0.1, 0.1 and 0.00203 m, respectively. Key words: Heat pipe heat exchanger, vapor compression refrigeration, closed loop oscillating heat pipe, optimum, thermo-economical method Published in Energy Research Journal 1 (2): 104-110, 2010. 165 Medical Consult Base System for Diagnosis on WiMAX System K. Noimanee, S. Noimanee, P. Khunja and P. Keawfoonrungsie Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT WiMAX (Worldwide Interoperability for Microwave Access) is a high-speed wireless broadband technology that was developed on the IEEE 802.16 standards and has developed to support mobile usage by setting a new standard IEEE 802.16e, the ability to send signals to spread from point-to-multipoint simultaneous with the ability to work in support Non-Line-of-Sight. This paper presents a measurement of ECG signals with teleconsult using IP video camera when the patient has symptom. The patient attached the sensor for measure ECG signals. The abnormal ECG signals of patient will send to IP video camera near patient in order to open for panning video camera of patient and being monitored by the physicians. The physicians can monitor patient using IP video camera through the web browser and ECG signals through the application via WiMAX technology. Key word: WiMAX, ECG Signal, IP Video camera Published in International Journal of Applied Biomedical Engineering.Vol.3, No.1, January-July 2010. pp. 51-55. 166 Microcalcification Detection in Mammograms Using Interval type-2 Fuzzy logic System with Automatic Membership Function Generation Suraphon Chumklin1, Sansanee Auephanwiriyakul2 and Nipon Theera-Umpon3 1 Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Computer Engineering, Faculty of Engineering, and is with Biomedical Engineering Center, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Electrical Engineering, Faculty of Engineering, Biomedical Engineering Center, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Breast cancer is an important deleterious disease. Mortality rate from this cancer is effectively high and rapidly increasing. The detection at the earlier state can help to reduce the mortality rate. In this paper, we apply the interval type-2 fuzzy system with automatic membership function generation using the Possibilistic C-Means (PCM) clustering algorithm. We utilize four features, i.e., B-descriptor, D-descriptor, average intensity of the inside boundary, and intensity difference between the inside and the outside boundaries. We also compare the result with the result from the interval type-2 fuzzy logic system with automatic membership function generation using the Fuzzy C-Means (FCM) clustering algorithm. The interval type-2 fuzzy system with PCM membership functions generation yields the best result, i.e., 89.47% correct classification with only 6 false positives per image. Published in the proceeding of Fuzzy Systems (FUZZ), 2010 IEEE International Conference on 18-23 July 2010, pp. 1-7, Barcelona, Spain. 167 Modeling of a rigid-flexible manipulator using Hamilton’s principle Santi Chanwikrai and Matthew O. T. Cole Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This paper presents a mathematical model of a two-link rigid-flexible manipulator, derived by Hamilton’s principle. The paper shows how the dynamic equations of motion can be simplified and converted to a form suitable for time-step integration. The equation describing deflection of the flexible end-link is discretized using the assumed modes method and a finite number of low frequency modes retained in the conversion to state-space form. A set of simulation results showing behavior of the model is presented. With the inclusion of a simple damping model, the simulation model gives physically realistic motion behavior and is therefore considered suitable for predicting vibration behavior of a rigid-flexible manipulator and as a tool for designing and testing controllers for reduction of flexible link vibration. Published in the Engineering Journal.CMU.[2010] 17 (3),19-27. 168 Modified Kinetic Model of Particle Detachment by Aerodynamic Drag and Vibration Nakorn Tippayawong and Ittichai Preechawuttipong Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This work is about detachment of deposited particles in turbulent air flow with external excitation. A kinetic model was modified and applied to the resuspension of microparticles. The modified model was based on the energy accumulation approach with combined aerodynamic drag and in-plane vibration to separate particles from a surface. Moments of adhesion, aerodynamic drag, and vibration acting on particles deposited to the surface were considered. An expression was obtained for the resuspension rate from surfaces where a spread of adhesive forces due to surface roughness was taken into account. The model prediction showed similar trends to the experiments. Frequency of particle-surface interaction was found to influence removal rate of particles from the surface. Published in the Proceeding on World Congress on Engineering and Computer Science (International Conference on Chemical Engineering), San Francisco, USA, 2010. 169 Moisture Content Prediction of Dried Longan Aril from Dielectric Constant Using Multilayer Perceptrons and Support Vector Regression Sanong Amaroek1, Nipon Theera-Umpon2,3, Kittichai Wantanajittikul3 and Sansanee Auephanwiriyakul3,4 1 Postharvest Technology Institute, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Biomedical Engineering Center, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Problem statement: Estimation of moisture contents of dried food products from their dielectric constants was an important step in moisture measurement systems. The regression models that provide good prediction performance are desirable. Approach: The Multilayer Perceptrons (MLP) and Support Vector Regression (SVR) were applied in this research to predict the moisture contents of dried longan arils from their dielectric constants. The data set was collected from 1500 samples of dried longan aril with five different moisture contents of 10, 14, 18, 22 and 25% Wet basis (Wb.) Dielectric constant of dried longan aril was measured by using our previously proposed electrical capacitance-based system. The results from the MLP and SVR models were compared to that from the linear regression and polynomial regression models. To take into account the generalization of the models, the four-fold cross validation was applied. Results: For the training sets, the average mean absolute errors over three bulk densities of 1.30, 1.45 and 1.60 g cm-3 were 1.7578, 0.6157, 0.3812, 0.3113, 0.0103 and 0.0044% Wb for the linear regression, second-, third-, fourth-order polynomial regression, MLP and SVR models, respectively. For the validation sets, the average mean absolute errors over the three bulk densities were 1.7616, 0.6192, 0.3844, 0.3146, 0.0126 and 0.0093% Wb for the linear regression, 2nd, 3rd and 4th-order polynomial regression, MLP and SVR models, respectively. Conclusion: The regression models based on MLP and SVR yielded better performances than the models based on linear regression and polynomial regression on both training and validation sets. The models based on MLP and SVR also provided robustness to the variation of bulk density. Not only for dried longan aril, the proposed models can also be adapted and applied to other materials or dried food products. Published in American Journal of Applied Sciences, Vol. 7, Issue 11, pp. 1387-1392, July 2010. 170 Non-isothermal pyrolysis characteristics of giant sensitive plants using thermogravimetric analysis Thanasit Wongsiriamnuay and Nakorn Tippayawong Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A giant sensitive plant (Mimosa pigra L.) or Mimosa is a fast growing woody weed that poses a major environmental problem in agricultural and wet land areas. It may have potential to be used as a renewable energy source. In this work, thermal behaviour of dried Mimosa was investigated under inert atmosphere in a thermogravimetric analyzer at the heating rates of 10, 30, and 50°C/min from room temperature to 1000°C. Pyrolysis kinetic parameters in terms of apparent activation energy and pre-exponential factor were determined. Two stages of major mass loss occurred during the thermal decomposition process, corresponding to degradation of cellulose and hemicellulose between 200-375°C and decomposition of lignin around 375-700°C. The weed mainly devolatilized around 200-400°C, with total volatile yield of about 60%. The char in final residue was about 20%. Mass loss and mass loss rates were strongly affected by heating rate. It was found that an increase in heating rate resulted in a shift of thermograms to higher temperatures. As the heating rates increased, average devolatilization rates were observed to increase while the activation energy decreased. Published in Bioresource Technology 101 (2010) 5638-5644. 171 Numerical Computation of Fluid Flow and Aerosol Transport in a Long Electrical Mobility Spectrometer P. Intra, L. P. Purba and N. Tippayawong Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Size distribution of submicron airborne particles can be effectively determined using electrical mobility technique. In this study, a numerical computation model for prediction of fluid flow and aerosol transport in a long column, electrical mobility spectrometer (EMS) has been developed. The internal 3D structure of an EMS [Intra and Tippayawong (2009), Korean J. Chem. Eng., 26(1), 269] was employed to simulate the complex flow patterns and aerosol particle trajectories in the EMS, including the swirling flow developed near the sheath air inlet slit. The incompressible Navier-Stokes equations were numerically calculated for the gas flow and particle trajectories, with a commercial computational fluid dynamics software package, FLUENT 6.3. The calculated results were found to agree well with previously published results in the literature. Prediction of fluid flow and aerosol transport was particularly useful in the EMS design and development. Published in International Journal of Engineering & Applied Sciences, Vol. 2 n. 1, pp. 16-26, 2010. 172 Optimal Choice and Allocation of Distributed Generations Using Evolutionary Programming Rungmanee Jomthong and Peerapol Jirapong Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT In this paper, evolutionary programming (EP) is proposed to determine the optimal choice and allocation of multi-type distributed generations (DG) to enhance power transfer capability and minimize system power losses of power transactions between source and sink areas in power systems. The optimal allocation includes the optimal type, size, and location. Two types of DG including photovoltaic (PV) and wind turbine (WT) are used in this study. The objective function is formulated as maximizing the benefit to cost ratio. The benefit means increasing in total transfer capability (TTC) with deducting system losses while the costs are the investment and operating costs of the selected DG units. Power transfer capability determinations are calculated based on the optimal power flow (OPF) technique. Test results on the modified IEEE 30-bus system show that the proposed EP can determine the optimal choice and allocation of DG to achieve the best TTC in the power system with the highest benefit to cost ratio. Key words: Distributed power generations, evolutionary programming, optimal power flow, optimal allocation Published in Proceedings of the IASTED International Conference, Power and Energy Systems (AsiaPES 2010) Phuket, Thailand. November 24-26, 2010. 173 Particulate Emission Reduction from Biomass Burning in Small Combustion Systems with a Multiple Tubular Electrostatic Precipitator Panich Intra1, Pravit Limueadphai2 and Nakorn Tippayawong2 1 College of Integrated Science and Technology, Rajamangala University of Technology Lanna, Chiang Mai 50200, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Biomass utilization via combustion is gaining an increasingly important role worldwide in heat and power production. Emissions of particulate matter from biomass burning have drawn considerable attention because of concern over their possible toxicity and implications for human health. For small-scale applications, it is desirable to employ a simple, compact, and inexpensive solution to avoid air quality problems related to biomass combustion. Electrostatic precipitation is a very reliable method to control particulate emissions from boilers, incinerators, and other industrial processes. It is therefore applied to small combustors. In this work, a simple, compact, and cost-effective multiple tubular electrostatic precipitator was designed and evaluated theoretically and experimentally for removal of particulate matter from a small biomass combustor. The precipitator consisted of a simple array of coaxial discharge electrodes placed along the axis of each cylindrical collection tube. The discharge electrodes were connected to the positive high-voltage supply, while the cylindrical collection tubes were grounded. The positive high-voltage supply was used to produce the corona discharge field between individual discharge electrode and collection tube. The particle-laden exhaust gas flow from the small biomass furnace was directed across the corona discharge field and then charged. The charged particles were deflected outward in a radial direction and deposited on the collection tube wall. The overall collection efficiency of the electrostatic device was evaluated as a mass loading ratio of the difference at inlet and outlet to the particle loading at the inlet of the device. It was found that about 70% overall collection efficiency can be achieved with a relatively simple multiple tubular electrostatic precipitator design. The device appeared to be a promising tool for reduction of particulate matter in flue gas from a small biomass combustion system. Key words: biomass burning, electrostatic precipitator, flue gas cleaning, particulate emissions, small combustor Published in Particulate Science and Technology, 28: 547-565, 2010. 174 PERFORMANCE AND EMISSION OF AGRICULTURAL DIESEL ENGINE USING PALM-DIESEL EMULSION AS FUEL Kitipong Nakpakdee1 and Tanonkiat Kiatsiriroat2 1 Department of Energy Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50202, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50202, Thailand. ABSTRACT Experimental study on agricultural diesel engine performance with emulsified oil has been carried out. In the study, palm-diesel oil with 5%v/v water, diese oil with 5% v/v water and pure diesel oil were tested in a single piston diesel engine having an auxiliary combustion chamber at a speed of 1000-2000 rpm. The results showed that the torques and the other performancefron all fuels gave similar values but CO, NOx and black smoke from the two emulsified oils were less than these of the diesel oil. The rate of fuel combustion from the palm-diesel emulsion was highest followed by diesel emulsion due to the higher viscosity and lower heating value compared with pure diesel. Key words: Palm-Diesel Emulsion, Diesel Engine, Performance, Emission Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.403, Chiang Mai, Thailand, November 2009. 175 Performance Evaluation of NTPv4 When SPIK State Cannot Step Frequency Yuthapong Somchit Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, Thailand. Abstract Network time protocol version 4 (NTPv4) is widely used to keep the time of NTPv4 client machine to be synchronized with the NTPv4 time server. NTPv4 does not adjust only the time but it also adjusts the frequency of the clock on client machine correspondingly to the NTPv4 server. In the NTPv4 specification, when time difference on NTPv4 client and server is greater than a threshold for a period of time, the NTPv4 is able to largely adjust the frequency at once. This action is called frequency stepping. However, in the real NTPv4 implementation, the frequency stepping was removed under this condition. This paper studies the effects of this missing action focusing on the time that NTPv4 client spends to adjust its clock frequency. The experiment was done under the scenario that NTPv4 client had to readjust its frequency. The results show that without frequency stepping, the NTPv4 spends time noticeably long to readjust its frequency. This ensures that NTPv4 should still use the frequency stepping for frequency readjustment in the real implementation. Published in ECTI-CON 2010, May 19-21, 2010. 176 Performance of Wire-on-tube Heat Exchanger Using OscillatingHeat Pipe Fin, A Case Study of Free Convection Tawat Samana1, Tanongkiat Kiatsiriroat1 and Atipoang Nuntaphan2 1 Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Thermal Technology Research Laboratory, Mae Moh Training Center, Electricity Generating Authority of Thailand, Mae Moh, Lampang 52220, Thailand. ABSTRACT The purpose of this research is to study the performance of wire-on tube heat exchanger using an oscillating heat pipe as its extended surface. R123 was selected as a working fluid of the oscillating heat pipe. The experiments were performed in a natural convection mode by exchanging heat between the hot water flowing inside the heat exchanger tube and the surrounding air. The inlet water temperature was varied between 40 and 85°C. The ambient temperature was kept constant around 25°C. It is found that the performance of wire-on-tube heat exchanger using an oscillating heat pipe fin is higher than that of a conventional wire-on-tube heat exchanger. Moreover the effect of heat exchanger setting position is also investigated. The result shows that horizontal plane heat exchanger has higher heat transfer performance than that of vertical plane. Published in the proceeding of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 177 Piezo Actuated Print Head System for Drop-On-Demand Printer Manatpong Mongkrai and Mana Saedan Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This paper discusses the development of the print-head system for drop-ondemand printer. Our drop-on-demand printer is being used for rapid prototyping applications that fabricate objects layer-bylayer. The advantage of the drop-on-demand printing over other existing techniques is that it can incorporate multi-materials to create a single object. Our print-head is made based on “squeeze mode” actuation that utilizes a piezo tube as an actuator. The print-head is designed to be nozzle or reservoir interchangeable. The result of experimental, print head can dropped on generate voltage = ±120 Volt. The print-head functional is verified using pure water is a printing media. The success of our development can be applied directly to multi-material dispensing. Key words: Print Head, Drop-On-Demand system, Rapid Prototype Published in the Proceeding on The 24th Conference of the Mechanical, Engineering Network of Thailand, October 20-22, 2010, Ubon Ratchathani. 178 POTENTIAL OF DENDROTHERMAL POWER GENERATION IN THAILAND AND ITS CONCERNS Abhinav Kulkarni1, Jenjira Piamdee2, Nat Vorayos2 and Weerachai Arjharn3 1 International Program Student, Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Agricultural Engineering, Suranaree University of Technology, Nakorn Rachasrima 30000, Thailand. ABSTRACT Fast rotation woods can grow rapidly within 3-5 years in plantation area and they give large amount of yield. Their high heating values are a good choice to use as fuel for electrification and energy. By using fast rotation wood as fuel in a form of woodchips, the Downdraft Gasification and Rankine Steam Power of approximately 100kw can sufficiently serve the demand in small villages of Thailand of around 100 dwellings. The environmental concerns are also needed to be taken into consideration and also the selling cost of electricity to the government power line. As fuel is taken from plantation instead of fossil-based source, this definitely can reduce greenhouse gas emissions in total. However, biodiversity can be taken into consideration on environmental impact if single species of fast rotation wood is used in vast area of wood fuel plantation. The results from basic calculation based on work at Chiang Mai University and Suranaee University of Technology in Thailand indicate that the Dendrothermal Power plant has high potential of being a success for sustainable development in small communities when the fuel wood plantation and transportation are well managed. Additionally, rotation of wood fuel plantation does help to cope with carbon dioxide in the Earth’s atmosphere but also helps to generate extra income to the agricultural community. Key words: Dendrothermal Powerplant, Rankine Steam System, Downdraft Gasifier, Mixed Plantation, Biodiversity Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.278, Chiang Mai, Thailand, November 2009. 179 Processing of hornblende syenite for ceramics Chairoj Rattanakawin1, Suraphol Phuvichit2, Yuenyong Panjasawatwong3 and Siwadol Supapia4 1 Department of Mining Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mining and Petroleum Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand. 3 Department of Geological Sciences, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Department of Mining Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this research is to preliminarily study of hornblende syenite processing. The study includes characterization, separation and evaluation. Characterization has been carried out using thin section, X-ray diffraction, X-ray fluorescence and electrokinetic measurement. A variety of techniques such as magnetic separation, froth flotation and combination of these techniques were used to separate feldspar from syenite. Evaluation of the separations has been done using data from yield of feldspar, X-ray fluorescence and cone firing test. The feldspar yield was used to evaluate the process efficiency. Besides, the chemical analysis, cone shrinkage, fired color and degree of vitrification were used to monitor the quality of the recovered feldspars. The feldspars were furthermore compared with the standard feldspar samples obtaining from a ceramic manufacturer. Finally, the processed feldspars were graded for using in various kinds of ceramics. Key words: ceramics, feldspar, hornblende syenite, mineral processing Published in Processing of hornblende syenite for ceramics Songklanakarin J. Sci. Technol., 32(2), 189-195, Mar.-Apr. 2010. 180 Production of Charcoal from Woods and Bamboo in a Small Natural Draft Carbonizer Nakorn Tippayawong, Nakarin Saengow, Ekarin Chaiya and Narawut Srisang Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT There is a strong domestic market for charcoal in Thailand and many developing countries. Charcoal is usually made from biomass materials in small scale, simple kilns. Traditional charcoal making kilns adopts a process that is very inefficient, and damaging to the environment. In this work, an alternative charcoal reactor based on natural draft, pyrolysis gas burning concept was proposed and demonstrated. Tests with woods and bamboo showed that good quality charcoal can be produced in shorter time with lower pollution emissions, compared with traditional kilns. The proposed carbonizer proved to be suitable for small scale, charcoal production in rural areas. Published in International Journal of Energy & Environment, Vol. 1 n. 5, pp. 911-918, 2010. 181 Production Cost Analysis in an Aluminum Die-Casting Department Using Activity-Based Costing System Dararat Pankan and Rungchat Chompu-inwai Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Product costs calculated through the use of traditional accounting methods are not based on criteria appropriate to the actual use of resources. As a result, employing activity-based costing will ensure that product costs are more accurate and are consistent with the use of resources. The method of analysis of this research began with the study of a traditional accounting system, in order to understand traditional product costing methods, then moved on to an analysis of product costing using an activity-based costing methodology, one which involves carrying out activity analysis, resource driver identification, activity-based costing, activity driver specification and activity cost allocation within the cost objects. By employing activity-based costing for those five products with the highest sales, it was found that two products incurred lower costs and the other three incurred higher costs than when using traditional costing methods. In addition, those products with the same production process incurred similar production costs. Product costs calculated using activity-based costing, are mainly based on actual activities, and this method recognizes that activities consume production resources. As a result, any product associated with a high number of activities will, in turn, incur higher production costs than one associated with a lower number of activities. Employing activity-based costing methods for product costing; therefore, is more consistent with the actual use of resources than traditional costing methods. Published in Engineering Journal CMU Vol. 17, No. 2, May-August, 2010. 182 Quantification of Liquid Water Saturation in a Transparent Single-Serpentine Cathode Flow Channel of PEM Fuel Cell by Using Image Processing S. Nirunsin and Y. Khunatorn Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The objective of this research is to quantify the water content on the cathode side in various PEM fuel cell operations. It can be revealed by direct visualization in an operational transparent single-serpentine PEM fuel cell. Images of liquid water accumulated inside the cathode flow channel were recorded by a digital camera to study water flooding in PEM fuel cell. The water coverage area in the cathode flow channel was estimated by an image processing technique. The effects of oxygen flow rate, cell temperature and time development on the water flooding were studied. The results indicated that excessive low or high cell temperature caused water to flood into the PEM fuel cell. The increasing of oxygen flow rate can remove more liquid water out of the cathode flow channel. However, too high oxygen flow rate caused the insufficient water content to maintain the membrane in the hydrated state and the dramatic decrease of fuel cell’s performance. The water flooding did not appear in a single-serpentine cathode flow channel when a transparent PEM fuel cell has operated within 40 minutes. Key words: PEM fuel cell; water management; direct visualization; transparent single-serpentine, image processing. Published in Journal of Sustainable Energy & Environment 1 (2010) 129-135. 183 RECOVERING AND UPGRADING WASTE HEAT OF AIR-CONDITIONER BY COMBINING R-123 VAPOR COMPRESSION HEAT PUMP Nattaporn Chaiyat and Tanongkiat Kiatsiriroat Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Experimental study of a vapor compression heat pump to recover waste heat from water-cooled condensers of 2 air-conditioners each of 12,000 BTU/h cooling capacity has been carried out. The waste heat could be upgraded for generating hot water up to 70°C. The AC EER of the airconditions for the water-cooled condenser could be 20% higher than that of the air-cooled units. But when the generating hot water is not used, the cooling water temperature should not be higher 45°C, otherwise, the water-cooled unit could not get an advantage in term of AC EER. A simplified model of the heat pump for recovering the waste heat from watercooled air conditioners in a hospital that need hot water around 815 litre/d. The payback is found to be 1.4 y compared with that the electric heater. Published in the proceeding of The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipment and Process. Prachuabkirikhan, 2010. 184 Selection of efficient wavelengths in NIR spectrum for determination of dry matter in kiwi fruit Lu Qiang1, Tang Mingjie1, Cai Jianrong1, Lu Huazhu1 and Sumpun Chaitep2 1 School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. 2 Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The feasibility of using efficient wavelengths in the near-infrared (NIR) spectrum for the rapid determination of the dry matter (DM) in kiwi fruit was investigated. Partial least squares (PLS), synergy interval PLS (siPLS) and genetic algorithm siPLS (GA-siPLS) were comparatively performed to calibrate regression models. The number of wavelengths and the number of PLS components were optimised as per the root mean square error of cross-validation (RMSECV) in the calibration set. The performance of the final model was evaluated by the root mean square error of prediction (RMSEP) and the correlation coefficient (r) in the prediction set. Results indicate that the performance of GA-siPLS model is the best one compared to PLS and siPLS models. The optimal model was achieved with r = 0.9020 and RMSEP = 0.5315 in the prediction set. This work shows that it is feasible to determine DM in kiwi fruit using NIR spectroscopy and that GAsiPLS algorithm is most suitable in solving the problem of selection of efficient wavelengths. Published in Maejo Internaitonal Journal of Science and Technology, Vol. 4 n. 1, pp. 113-124, 2010. 185 Simulation of Cassava Chip Drying using Convective Hot-Air Tossaporn Wimuktacom and Aree Achariyaviriya Department of Mechanical Engineering, Facutly of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this research was to determine the equation of drying constant and to develop a model for the drying system of cassava chip using hot air. Cassava chip was classified into three sizes (small, medium and large). Thin-layer drying experiments were carried out at drying air temperatures of 40, 50, 60, 70 and 80°C with an air velocity of 2.3 m/s. The drying equation evaluated in this study included the semi - theory and empirical equations (Page and Henderson & Pabis models) where theirs drying constants were represented as a function of drying air temperature and product size. From the results, it was found that Page model provided the best to fit of data. A near -equilibrium model of drying system of cassava chip was used to predict the drying rate and specific energy consumption and validate with experimental data. Comparisons showed that simulated results were in agreement with experimental values. The simulated results also indicated that the drying rate decreased and specific energy consumption increased as the drying air temperature and cassava chip size increased. Published in the Engineering Journal.CMU.[2010] 17 (1),32-41. 186 Temperature Reduction in Hydroponic Planting System Using Heat Pipe Paramet Suttiprapa, Phurt Sakulchangsatjatai, Nednapa Insalud and Pradit Terdtoon Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract This paper presents an establishment of a mathematical model to determine the optimal size of heat pipe (thermosyphon) heat exchanger for educe the inlet air temperature in evaporative cooling system of greenhouse. The condition of a mathematical model is the total length of heat pipe is 1.5 m. A mathematical model will determine the optimal value of evaporator section and condenser section. The range of evaporator length is 0.5-1.4 m with an icrement of 0.1 m and condenser length is in range of 0.1-1.0 m with an increment of 0.1 m. The inlet air temperature in evaporative cooling of greenhouse is depend on ambient temperature about 35 degree Celsius. While, the cooling water temperature is 20 degree Celsius. Evaporative pad of evaporative cooling system is 2 m in width and 7 m in leghth. From the conditions above, the optimize results from a mathematical model are as follows: evaporater section length is 0.9 m and condense section length is 0.6 m moreover, it was shown that investment, net saving and maximum heat rate are 79.83 Thousand Baht, 0.552 Million Baht and 15.6 kW respectively. Key Words: Evaporative Cooling, Greenhouse, Heat Pipe, Hydroponic System. Thermosyphon. Published in the Proceedings of The 2nd RMUTP International Conference Green Technology and Productivity, pp 90-95, Bangkok, Thailand, 29-30 June 2010. 187 THE ANALYSIS OF ENERGY CONSUMPTION AND CARBON DIOXIDE EMISSIONS IN THAI MANUFACTURING SECTOR BY DECOMPOSITION TECHNIQUE Yasintinee Aimyuak, Wongkot Wongsapai and Chatchawan Chaichana Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Muang, Chiang Mai 50200, Thailand. ABSTRACT Manufacturing sector is the most effect in Thai energy consumption and CO2 emissions for many years. This paper shows an analysis of the change in energy intensities and CO2 emission intensities from manufacturing sector energy consumption by decomposition method type AMD(Arithmetic Mean Divisia index) and LMD (Logarithmic Mean Divisia index). These methods present the effect of energy intensities, the effect of the changes in structure and the effect of the economic growth on the energy consumption data from 1981 to 2007. The analysis is carried out in four phases; i.e. (i)pre-economic boom (ii)economic boom, (iii) Asian economic crisis, (iv)third world energy crisis. The results indicate that both energy and CO2 intensities have increased in period of economic boom (1989 to 1996). Then, during the economic crisis (1997 to 2004) both intensities declined due mostly to a fall in energy intensities. The last period, both intensities fell due to structural fuel mix effects and the rising of the energy price. The analysis also indicated that non-metallic and basic metal manufacturing sub-sector play an important role to energy consumption and the change in both intensities. Hence, the national energy conservation play should be placed on the energy intensive sector by proper policy recommendation and measures implementation. Key words: Energy decomposition, Carbon dioxide decomposition, Manufacturing sector, Thailand Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.287, Chiang Mai, Thailand, November 2009. 188 The design and performance of constructed wetlands for wastewater treatment in tropical regions S. Kantawanichkul Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Tropical climate provides good opportunity for the success of treatment wetlands as high temperature influencing the biological degradation and the growth of macrophytes. Therefore, high temperature could be beneficial for less land requirement and higher hydraulic loading application to the system. In the last 10 years the number of constructed wetlands are increasing in tropical region especially in Asia. Up to 2006, approximately 82 full scale constructed wetlands have been implemented in Taiwan. The design of constructed wetlands in Taiwan has been integrated into the development of green architecture so they are also provided as a recreation areas. In Singapore, there are 19 wetland projects and most of them are for storm water and reservoir water treatment. The largest constructed wetland in the tropic is in Malaysia called Putrajaya and was implemented for storm water treatment. In Thailand, the constructed wetland was introduced by the king Bhumibol in 1992. However, it has not become very popular. It was found that most of the constructed wetlands have been left without good operation and maintenance. The failures were caused by many reasons such as the lack of local people participation, regular budget for operation and maintenance and knowledge for the design and construction. Published in a proceedings on the 12th International Conference on Wetland Systems for Water Pollution Control. pp.23, Venice, Italy, October, 2010. 189 THE STUDY OF BIOCHAR PROPORTY FROM SLOW PYROLYSIS OF ALGAE Kanyaporn Chaiwong1, Tanongkiat Kiatsiriroat2 and Churat Thararux3 1 Energy Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Faculty of Engineering, Rajamangala University of Technology Lanna, Chiang Mai, Thailand. ABSTRACT Biochar is an acceptable technology for carbon storage in the earth produce. It has a potential decrease CO2 in the atmosphere and solve the global warming situation. This research proposes the biochar production from algae with slow pyrolysis process in a bed reactor at 550°C. The results are found that the produced biochar from this process has a property nearly to that from other biomass resource at the same condition. The comparison results is found the fixed carbon composition in the biochar of Cladophora spp. gives high component of carbon of 59.66%, this value is higher than those of Spirulina spp. and Sprogyra Spp.,. Moreover, the fuel properties are similar to those of fossil fuel and Oak biochar. Published in the Proceedings of the 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipments and Process. Prachuabkirikhan, 2010. 190 The Study of Maximum Load of Thin-wall Paper Tube Under Axial Compressive Load Piyaphong Pantana and Thongchai Fongsamootr Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This paper presents the study on the maximum static axial compressive load of thin-wall paper tube. The experiment, which used universal materials testing machine: Instron series 5566, with a constant compression rate 2 mm/ min, and theory calculation will be studies and then the results were compared. The specimens were created from Kraft-Paper. There were 3 values of radii, which are 25, 35 and 50 millimeters, and height that are 100, 200 and 300 millimeters. The specimens were divided to 3 types: 125, 185 and 230 gram. The thicknesses of each type were 0.157, 0.236 and 0.298 mm, respectively. And compressive load were applied in machine and cross machine direction of paper. The results showed that the maximum compressive load of experiment and theory calculation were consistent trend. And thickness had the most influenced on the maximum compressive load. Published in the Engineering Journal.CMU.[2010] 17 (2),1-9. 191 THE TEST OF A MODULAR CHEMICAL ENERGY STORAGE SYSTEM USING SODIUMSULPHIDE-WATER AS WORKING PAIR Prachaya Siriya and Wipawadee Wongsuwan Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Energy storage system is one that relevant to the increasing of energy efficiency of industrial sector using thermal energy. This research reported the test results of operating cycle of a chemical energy storage using sodium sulfide-water working pair to enhance compactness. The analysis focus on the effect on system performance, i.e., coefficient of performance for heating (COPh), specific heat storage power (SHP) and volumetric heat production (VHP), if varying evaporator temperature (25-45°C) and temperature of heat transfer fluid during decomposition process (80, 90°C). The operating pressure was varied in the range of 3-10 kPa. In summary, raising evaporator temperature results in higher the COPh and SHP, however, in contrary to the effect of increasing heat source temperature. The maximum obtained COPh was 1.77. Therefore, this small chemical energy storage module has possibility to be enlarged as greater system having several modules. Published in the proceeding of the 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipments and Process. Prachuabkirikhan, 2010. 192 Thermal characteristics of louvered fins with a low-reynolds number flow Nat Vorayos and Tanongkiat Kiatsiriroat Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A heat recovery system is crucial for the effective use of energy where heat rejection from production processes is unavoidable and must be reused. The response of the louvered fins to the low-Reynolds number hot gas is yet to be reported in the literature for the application of a heat exchanger on low-speed hot plume arising from heat sources in production processes. This study focuses on the effects of the louvered fin heat exchangerís design parameters, which include the louver pitch and louver angle, on the convective heat transfer, which defines the thermal interaction between the hot, buoyant, naturally-induced air and the louvered fins. The resulting Colburn factors (j) are compared with those derived under forced convection with a similar range of low Reynolds number (233 to 1024). All experiments are done on a 15:1 scaled-up model. The fin aspect ratios between the fin spacing and louver pitch are set at 0.75, 1, and 1.5, while the louver angles are set at 18°, 23°, 30°, 35°, and 40°. The Colburn factor strongly depends on the louver angle, especially at the lower range of the Reynolds number. The decreasing aspect ratio induces more hot buoyant air into the louver-formed channels, increasing the heat transfer rate. When the fin angle increases towards 30°, a larger Colburn factor is produced. However, the heat transfer characteristic drops as the angle goes beyond 30°. The highest j for the low speed flow is attained when the louver angle is 30° and the fin aspect ratio is 1. Published in Journal of Mechanical Science and Technology, Vol. 2010 n. 24, pp. 1-6, 2010. 193 THERMAL EFFICIENCY OF FIRE TUBE BOILER USED MAYAOHIN BIODIESEL AS A FUEL Kitipong Nakpakdee1, Tanongkiat Kiatsiriroat2, Vorapoj Pothacharoen3, Kanyaporn Chaiwong3 and Nuttawut Dussadee4 1 Department of Mechanical Engineering, Faculty of Engineering, Rajamangala University of Technology Lanna, Chiang Mai, Thailand. 2 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Energy Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Energy Research Center, Maejo University, Chiang Mai 50210, Thailand. ABSTRACT Vernicia montana Lour, named in Thai as Mayaohin, is an oil free of which the crude oil could be transformed into bio-diesel. This work applies the crude oil in the forms of bio-diesel. This work applies the crude oil in the forms of bio-diesel and emulsion are used as fuel in a boiler for studying the thermal efficiency and emission. The results show that the emulsified fuel give the boiler efficiency of 66.94% compared with 69.00% for diesel oil and 62.28% from the biodiesel. Besides, the nitrogen oxide emission (NOx) from the emulsified oil and the biodiesel could be reduced 15.58%, and 5.20%, respectively, from that from the diesel fuel. However, the fuel consumption of the biofuels in both forms are higher than that from the diesel. Published in the Proceedings of the 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipments and Process. Prachuabkirikhan, 2010. 194 Thermogravimetric analysis of giant sensitive plants under air atmosphere Thanasit Wongsiriamnuay and Nakorn Tippayawong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT The aim of this work is to utilise thermal analysis to study the thermal degradation of giant sensitive plants (Mimosa pigra L.) or Mimosa under oxidative environment. Thermogravimetric method was used under air sweeping in dynamic conditions at the heating rates of 10, 30, and 50°C/min, from room temperature to about 725°C. Starting with dehydration step between 30 and 150°C, the main thermal decomposition process under air showed two distinct degradation zones, corresponding to devolatilisation step between 200 and 37°C and combustion step around 375-500°C. Kinetic parameters in terms of apparent activation energy and pre-exponential factor were determined. Comparison was made against other biomass materials. Mass loss and mass loss rates were strongly affected by heating rate. It was found that an increase in heating rate resulted in a shift of thermograms to higher temperatures. As the heating rates increased, average devolatilisation and combustion rates were observed to increase while the activation energy showed slight increase. Published in Bioresource Technology 101 (2010) 9314-9320. 195 UPGRADING OF LOW TEMPERATURE SOLAR HEAT FOR HIGHER TEMPERATURE APPLICATION AND COOLING Nattaporn Chaiyat and Tanongkiat Kiatsiriroat Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Solar energy is a clean energy and its application could reduce global warming potential (GWP) effect. In this study, improvement of low temperature heat at 60-90 from common flat-plats solar collector is upgraded to around 120 is by Water-Liber heat transformer is presented. The high temperature heat could be taken as a heat source for electricity generation through an organic rankine cycle (ORC) or for cooling by an absorption chiller. Key words: Solar energy, heating, cooling, Absorption chiller, Heat transformer Published in the Proceedings of the 5th Academic Days “Research Path: The Fifth Decade Towards a University of Excellence” pp.402, Chiang Mai, Thailand, November 2009. 196 Use of oscillating heat pipe technique as extended surface in wire-on-tube heat exchanger for heat transfer enhancement Atipoang Nuntaphan1, Sanparwat Vithayasai2, Nat Vorayos2, Nattanee Vorayos2 and Tanongkiat Kiatsiriroat2 1 Electricity Generating Authority of Thailand, Mae Moh, Lampang, Thailand. Technology Licensing Office, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT This paper presents the performance of a wire-on-tube heat exchanger of which the wire is an oscillating heat pipe. The experiments for this heat exchanger were performed in a wind tunnel by exchanging heat between hot water flowing inside the heat exchanger tubes and air stream flowing across the external surface. R123, methanol and acetone were selected as working fluids of the oscillating heat pipe. The inlet water temperature was varied from 45 to 85°C while the inlet air temperature was kept constant at 25°C. The results show that the oscillating heat pipe technique for all working fluids could increase around 10% of the heat transfer rate obtained from that without the refrigerant flow in the capillary tube when the inlet water temperature is at 60°C. Moreover, the heat transfer models for evaluating the heat exchanger effectiveness and the air-side heat transfer coefficient, are also developed in this work. These results of the models agreed very well with the experimental data. Published in Heat Transfer Engineering, Vol. 2010, 2010. 197 WATER QUALITY VARIATION IN A POND RAISING TILAPIA CAGE CULTURE Somjai Karnchanawong1, Nantawan Saengroj1, Prapatsorn Lohwanta1 and Noppadol Kongsricharoen2 1 Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Thai Environmental and Energy Development Company, 28/6 Thavornvilla, Moo 7, Lumlooka Road, Tumbol Ladsavai, Amphoe Lumlooka, Pathumthani 12150, Thailand. ABSTRACT The objectives of this study were to determine the water quality in the pond raising tilapia culture in floating net cage. In addition the BOD, N and P generation rates from fish raising were also evaluated. Five hundreds of Tilapias with 5 centimeter length were released into the cage (2.0 m width, 6.0 m length, and 1.0 m depth) installed in a pond (18 m width, 18 m length, and 3.0 m depth) and were fed twice a day with commercial floating granule food for 7 months. Twelve cubic meters of water from Kuang River was pumped into the pond once a week from the 3rd week after raising. The water in the pond were sampled and analyzed for temperature, pH, conductivity, dissolved oxygen, BOD, NO2-N, NO3-N, NH3-N, Org-N and TP. The results revealed that the Ti lapia culture in floating net cage resulted in the decreasing of dissolved oxygen and increasing of BOD and N in the water. However, the increasing of TP content in the water was not observed. The BOD and N generation rate from the tilapia culture were 4.34 and 3.4 g/kg fish production, respectively. Key words: Tilapia, water quality, dissolved oxygen, BOD, nitrogen Published in the Proceedings on the 9th National Environmental Conference, 24-27 March 2010. Ubonratchatani. 198 2-[3-(2-Aminoethylsulfanyl)propylsulfanyl]ethanamine Bearing Dansyl Subunits: An Efficient, Simple, and Rapid Fluorometric Sensor for the Detection of Mercury(II) Ions Wanichacheva Nantanit1, Kamkaew Anyanee1, Watpathomsub Supranee1, Lee Vannajan Sanghiran2,3 and Grudpan Kate3 1 Silpakorn University, Department of Chemistry, Faculty of Science, Nakhon Pathom 73000, Thailand. 2 Communication of Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 3 Department of Chemistry, Center of Innovation in Chemistry, Faculty of Science, Chiang Mai 50200, Thailand. Abstract A novel fluorometric sensor possessing two dansyl moieties based on 2-[3-(2-aminoethylsulfanyl)propylsulfanyl]ethanamine 1 was prepared by a wady synthetic approach using a conventional two-step synthesis I exhibits highly sensitive and selective on-off Hg(2+) fluorescence quenching behavior in aqueous acetonitrile solutions and is shown to discriminate various competing metal ionssuch as Cu(2+), Ag(+), Ca(2+), Mn(2+), Zn(2+), Ba(2+), Cd(2+), Co(2+), Na(+), and K(+) exhibiting a detection limit of 7 nM or 1.4 ppb with a working range of 1.8-15 ppb. Key words: SELECTIVE FLUORESCENT CHEMOSENSOR; PHOTOPHYSICAL PROPERTIES; CATION-BINDING; AQUEOUS-MEDIA; FLUOROIONOPHORE; FLUOROPHORE; CROWN; FISH; CHROMOIONOPHORE; BEHAVIORS CHEMISTRY LETTERS Volume: 39 Issue: 10 Pages: 1099-1101 DOI: 10.1246/cl.2010.1099 Published: OCT 5 2010. 199 A Barium-Calcium Silicate Glass for Use as Seals in Planar SOFCs Namwong Prachaya2, Laorodphan Nattapol1, Thiemsorn Worapong1, Jaimasith Manat1, Wannakon Anucha3 and Chairuangsri Torranin1 1 Department of Industrial Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 National Metal and Materials Technology Center, Bangkok 12120, Thailand. Abstract A barium-calcium silicate glass for use as seals in planar SOFCs with a chemical composition of 40.78mol%BaO-4.36mol%CaO-0.62mol%Al(2)O(3)-0.83mol%B(2) O(3)-47.41mol% SiO(2) was studied. Its coefficient of thermal expansion (CTE) between 25-640 degrees C, glass transformation temperature (T g) and softening temperature (T(s)), determined by dilatometry, were 10.6x10(-6)degrees C(-1), 646 degrees C and 694 degrees C, respectively. Isothermal devitrification heat treatment at 800 degrees C, which is in the intermediate operation temperature range of planar SOFCs, revealed surface crystallization of various crystalline phases identified by XRD and SEM-EDS as BaAl(2)Si(2)O(8) (hexacelsian), BaSiO(3) and BaCa(2)Si(3)O(9). Kinetics of devitrification followed the Johnson-Mehl-AvramiKolmogorov relationship with the Avrami exponent of 1.4. The overall CTE of the obtained glass-ceramic after the isothermal devitrification heat treatment was comparable to that of the glass. Adhesion test between the glass and the other components including YSZ and stainless steel grade AISI430 revealed that good union was achieved. The interfacial phenomena were studied by SEM-EDS and the mechanism of bonding were discussed. Key words: OXIDE FUEL-CELLS; CRYSTALLIZATION KINETICS; CERAMIC SEALANT; NUCLEATION; GROWTH; ZN; MG CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 2 Pages: 231-242 Published: MAY 2010. 200 A Hybrid Method for a Countable Family of Multivalued Maps, Equilibrium Problems, and Variational Inequality Problems Cholamjiak Watcharaporn1,2 and Suantai Suthep1,2 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Mahidol University, PERDO National Center of Excellence in Mathematics, Faculty of Science, Bangkok 10400, Thailand. Abstract We introduce a new monotone hybrid iterative scheme for finding a common element of the set of common fixed points of a countable family of nonexpansive multivalued maps, the set of solutions of variational inequality problem, and the set of the solutions of the equilibrium problem in a Hilbert space. Strong convergence theorems of the purposed iteration are established. Key Words: STRONG-CONVERGENCE THEOREMS; NONEXPANSIVE-MAPPINGS; BANACH-SPACES; FIXED-POINTS; MANN; WEAK; SEMIGROUPS DISCRETE DYNAMICS IN NATURE AND SOCIETY Article Number: 349158 DOI: 10.1155/2010/349158 Published: 2010 201 A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer Thongchai Wisanu2, Liawruangath Boonsom2, Liawruangrath Saisunee3,4,5 and Greenway, Gillian M.1 1 University of Hull, Department of Chemistry, Kingston Upon Hull HU6 7RX, N Humberside, England. 2 Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Center of Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 5 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A novel chemiluminescence (CL) microfluidic system incorporating a molecularly imprinted polymer (MIP) preconcentration step was used for the determination of chloramphenicol in honey samples. The MIP was prepared by using chloramphenicol as the template, diethylaminoethyl methacrylate (DAM) as the function monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking monomer, 2, 2’-dimethoxy-2-phenylacetophenone (DMPA) as the free radical initiator and toluene and dodecanol as the solvent. The MIP was pre-loaded into a 10 mm long, 2 mm wide and 1501,,m deep channel in a planar glass microfluidic device. When the sample containing chloramphenicol was introduced into the microfluidic device it was first preconcentrated on the MIP then detected by an enhancement effect on the chemiluminescence reaction of tris(2, 2’-bipyridyl) ruthenium(II) with cerium(IV) sulphate in sulphuric acid. A micro-syringe pump was used to pump the reagents. The CL intensity was linear in relationship to the chloramphenicol concentrations from 1.55 x 10(-4) to 3.09 x 10(-3) mu mol L(-1) (r(2) = 0.9915) and the detection limit (3 sigma) and the quantitation limit (10 sigma)were found to be 7.46 x 10(-6) and 2.48 x 10(-5) mu mol L(-1), respectively. This method offered a high selectivity and sensitivity for quantitative analysis of chloramphenicol in the honey samples. (C) 2010 Elsevier B.V. All rights reserved. Key words: Chloramphenicol; Molecularly imprinted polymer (MIP); Chemiluminscence; Microfluidics; MicroFIA TALANTA Volume: 82 Issue: 2 Pages: 560-566 DOI: 10.1016/j.talanta.2010.05.007 Published: JUL 15 2010. 202 A Multiple Processing Hybrid Flow System for Analysis of Formaldehyde Contamination in Food Teerasong Saowapak2, Amornthammarong Natchanon3, Grudpan Kate4, Teshima Norio5, Sakai Tadao5, Nacapricha Duangjai6,7 and Ratanawimarnwong Nuanlaor1 1 Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand. 2 Institute for Innovation Learning, Mahidol University, Nakhon Pathom 73170, Thailand. 3 NOAA, Ocean Chemistry Division AOML, Miami, FL 33149 USA. 4 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 5 Department of Applied Chemistry, Aichi Institute of Technology, Toyota 4700392, Japan. 6 Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. 7 Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Abstract This work proposes a flow system suitable for the rapid screening of formaldehyde contaminated in food. The system is based on the concept of a flow analyzer with a Hantzsch reaction. An operating procedure was developed for multiple tasking and high sample throughput. This resulted in a significant sample throughput of 51 samples h(-1). Under the optimized conditions, linear calibration from 10 to 100 mu M was obtained. The system gave a limit of detection and a limit of quantitation of 0.06 and 0.10 mg kg(-1), respectively. The system was successfully applied to re-hydrated dry squids, vegetables and mushrooms. Key Words: SENSITIVE DETERMINATION; RAPID-DETERMINATION; TRACE FORMALDEHYDE; INJECTION ANALYSIS; BEVERAGES; ANALYZER; FISH; GAS; AIR ANALYTICAL SCIENCES Volume: 26 Issue: 5 Pages: 629-633 Published: MAY 2010. 203 A new approximation method for common fixed points of a finite family of asymptotically quasi-nonexpansive mappings in Banach spaces Kettapun Atichart, Kananthai Amnuay and Suantai Suthep Deparment of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In this paper, we consider a new iterative scheme to approximate a common fixed point for a finite family of asymptotically quasi-nonexpansive mappings. We prove several strong and weak convergence results of the proposed iteration in Banach spaces. These results generalize and refine many known results in the current literature. (c) 2010 Elsevier Ltd. All rights reserved. Key words: Modified Mann and Ishikawa iterations; Asymptotically quasi-nonexpansive mappings; Common fixed points; Uniformly convex Banach spaces COMPUTERS & MATHEMATICS WITH APPLICATIONS Volume: 60 Issue: 5 Pages: 14301439 DOI: 10.1016/j.camwa.2010.06.025 Published: SEP 2010. 204 A New Approximation Method for Common Fixed Points of a Finite Family of Generalized Asymptotically Quasinonexpansive Mappings in Banach Spaces Yatakoat Pornsak and Suantai Suthep Deparment of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract We introduce a new iterative scheme to approximate a common fixed point for a finite family of generalized asymptotically quasinonexpansive mappings. Several strong and weak convergence theorems of the proposed iteration in Banach spaces are established. The main results obtianed in this paper generalize and refine many known results in the current literature. Key Words: QUASI-NONEXPANSIVE MAPPINGS; IMPLICIT ITERATION PROCESS; STRONG-CONVERGENCE THEOREMS; VISCOSITY APPROXIMATION; ERRORS; MAPS ABSTRACT AND APPLIED ANALYSIS Article Number: 706587 DOI: 10.1155/2010/706587 Published: 2010. 205 A New Azwafluorenone from the Roots of Polyalthia cerasoides and its Biological Activity Pumsalid Kanchana1,2, Thaisuchat Haruthai1,2, Loetchutinat Chatchanok3, Nuntasaen Narong4, Meepowpan Puttinan5,6 and Pompimon Wilart1,2 1 Natural Products Laboratory, Faculty of Science, Lampang Rajabhat University, Lampang 52100, Thailand. 2 Center for Innovation in Chemistry, Lampang Rajabhat University, Lampang 52100, Thailand. 3 Laboratory of Physical Chemistry Moleculan and Cellular Biolgy (PCMCB), Center of Excellence for Molecular Imaging, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Department of National Park Wildlife & Plant Conservation, Ministry of Natural Resources & Environment, Forest Herbarium, Bangkok 10900, Thailand. 5 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50300, Thailand. 6 Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50300, Thailand. Abstract Chromatographic separation of the ethyl acetate extract of roots of Polyalthia cerasoides has led to the isolation of the new compound, 6,8-dihydroxy-7-methoxyl-methyl-azafluorenone. This compound exhibited potent cytotoxic activities with IC(50) values in the range of 2.64-3.58 mu g.mL(-1) for A549, GLC4 and GLC4/Adr cells, but was not recognized by ABCC1/MRP1 protein. The compound also showed very strong inhibition of M tuberculosis using a broth microdilution method, with an MIC value of 0.78 mu g.mL(-1), which was equal to that of ofloxacin, one of the four antibiotic drugs used as a positive control. Key Words Plus: CONSTITUENTS NATURAL PRODUCT COMMUNICATIONS Volume: 5 Issue: 12 Pages: 1931-1934 Published: DEC 2010. 206 A New General Iterative Method for a Finite Family of Nonexpansive Mappings in Hilbert Spaces Singthong Urailuk1 and Suantai Suthep1,2 1 Departmant of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Faculty of Science, PERDO National Center of Excellence in Mathematics, Mahidol University, Bangkok 10400, Thailand. Abstract We introduce a new general iterative method by using the K-mapping for finding a common fixed point of a finite family of nonexpansive mappings in the framework of Hilbert spaces. A strong convergence theorem of the purposed iterative method is established under some certain control conditions. Our results improve and extend the results announced by many others. Key Words: STRONG-CONVERGENCE; THEOREMS FIXED POINT THEORY AND APPLICATIONS Article Number: 262691 DOI: 10.1155/2010/262691 Published: 2010. 207 A role of BNLT compound addition on structure and properties of PZT ceramics Jaita P., Watcharapasorn A. and Jiansirisomboon S. Department of Physic & Meterials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In this research, effects of lead-free bismuth sodium lanthanum titanate (BNLT) addition on structure and properties of lead zirconate titanate (PZT) ceramics were investigated. PZT ceramics with addition of 0.1-3.0 wt%BNLT were fabricated by a solid-state mixed oxide method and sintering at 1050-1200 degrees C for 2 h to obtain dense ceramics with at least 96% of theoretical density. X-ray diffraction indicated that complete solid solution occurred for all compositions. Phase identification showed both tetragonal and rhombohedral perovskite structure of PZT with no BNLT phase detected. Scanning electron micrographs of fractured PZT/BNLT ceramics showed equiaxed grain shape with both transgranular and intergranular fracture modes. Addition of BNLT was also found to reduce densification and effectively limited grain growth of PZT ceramic. Optimum Hv and K(IC) values were found to be 4.85 GPa and 1.56 MPa.m(1/2) for PZT/0.5 wt%BNLT sample. Among PZT/BNLT samples, room temperature dielectric constant seemed to be improved with increasing BNLT content. The maximum piezoelectric coefficient values were observed in pure PZT ceramic and were slightly decreased in BNLT-added samples. Small reduction of remanent polarization and coercive field in hysteresis loops was observed in BNLT-added samples, indicating a slightly suppressed ferroelectric interaction in this material system. (C) 2010 Elsevier Masson SAS. All rights reserved. Key words: PZT; BNLT; Mechanical properties; Dielectric; Piezoelectric; Ferroelectric SOLID STATE SCIENCES Volume: 12 Issue: 9 Pages: 1608-1614 DOI: 10.1016/j.solidstatesciences.2010.07.008 Published: SEP 2010. 208 Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production Ruanpanun Pornthip1,2, Tangchitsomkid Nuchanart3, Hyde Kevin D.4 and Lumyong, Saisamorn1,2 1 Division of Microbiology, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Biotechnology Program, Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Agriculture, Nematology Section, Plant Protection Research & Development Office, Bangkok 10900, Thailand. 4 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Abstract Root-knot nematodes are serious pathogens that severe damage to major crops. They damage plant root system that caused significant yield losses. Moreover, the predisposition of nematode-infected plants is secondary infection from fungal plant pathogen that additional adverse effects on plant growth. Our target is to find the antagonist for control nematode, and secondary infection agents and stimulate plant growth. Twenty-three plant-parasitic nematode infested soils were taken from some provinces in the northern and center of Thailand and actinomycetes and fungi were isolated. Eighty-three isolates belong to actinomycete and 67 isolates were fungi. The predominant actinomycete taxa was Streptomyces (97.6%). The predominant fungal taxa were Penicillium (37.3%) and Fusarium (32.8%). All actinomycete and fungal isolates were subjected for primary screening in vitro for their effects on egg hatching and juvenile mortality of Meloidogyne incognita. Secondary screening was evaluated for antagonist effect on plant pathogenic fungi collected from nematode-infected plant, plant growth hormone (indole-3-acetic acid; IAA) and siderophore production. From primary screening, 7 actinomycete and 10 fungal isolates reduced egg hatching and kill juveniles of M. incognita after 7 days incubation. In secondary screening, 10 nematophagous microbes produced IAA and 9 isolates produced hydroxamate siderophore. Streptomyces sp. CMU-MH021 was selected as a potential biocontrol agent. It reduced egg hatching rate to 33.1% and increased juvenile mortality rate to 82% as contrasted to the control of 79.6 and 3.6%, respectively. This strain had high activity to against tested fungi and high ability on IAA (28.5 mu g ml(-1)) and siderophore (26.0 mu g ml(-1)) production Key words: Actinomycetes; Fungi; Root-knot nematode; Biocontrol; Siderophore; Indole-3-acetic acid WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY Volume: 26 Issue: 9 Pages: 1569-1578 DOI: 10.1007/s11274-010-0332-8 Published: SEP 2010. 209 Age Hardening and Precipitation Behavior of an Experimental Cast Al-Mg-Si Alloy Treated by T6 and T6I6 Heat Treatments Imurai Suttawan1, Kajornchaiyakul Julatep2, Thanachayanont Chachana2, Pearce, John T. H.2 and Chairuangsri Torranin1 1 Department of Industrial Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 National Metel & Materials Technology Center, Bangkok 10400, Thailand. Abstract studied. Age hardening was performed by two artificial ageing heat treatments, T6 and T6I6. Vickers microhardness was measured within the dendritic regions. It was found that peak ageing was reached after about 24 hours. T6 and T6I6 increased the hardness from the as-cast value by 52% and 60%, respectively. The peak hardness achieved via T6I6 is approximately 8% higher than that obtained by T6. Overall microstructure was studied by scanning electron microscopy and energy dispersive x-ray spectrometry. Eutectic constituents were revealed as Si flakes and Fe-bearing intermetallic compounds including pi-Chinese script phase (Al(8)Mg(3)FeSi(6)) and beta(Al(5)FeSi). Precipitation behavior was investigated by transmission electron microscopy and the precipitation sequence found was somewhat in agreement with those reported previously for wrought and cast Al-Mg-Si alloys. However, the peak hardness ageing period coincided with the presence of beta ‘phase or its precursors, not beta’ phase. Key Words Plus: TRANSMISSION ELECTRON-MICROSCOPY; ALUMINUM-ALLOY; A356/357; SEQUENCE; TENSILE; SYSTEM; PHASE CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 2 Pages: 269-281 Published: MAY 2010. 210 An iterative method for equilibrium problems and a finite family of relatively nonexpansive mappings in a Banach space Cholamjiak Prasit and Suantai Suthep Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In this paper, we introduce a new iterative method for finding a common element of the set of fixed points of a finite family of relatively nonexpansive mappings and the set of solutions of an equilibrium problem in uniformly convex and uniformly smooth Banach spaces. Then we prove a strong convergence theorem by using the generalized projection. (C) 2010 Elsevier Inc. All rights reserved. Key Words: STRONG-CONVERGENCE THEOREMS; WEAK; ERRORS APPLIED MATHEMATICS AND COMPUTATION Volume: 217 Issue: 8 Pages: 3825-3831 DOI: 10.1016/j.amc.2010.09.041 Published: DEC 15 2010. 211 Analysis of lead molybdate and lead tungstate synthesized by a sonochemical method Phuruangrat Anukorn1, Thongtem Titipun2,3 and Thongtem Somchai1 1 Department of Physic & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Lead molybdate and lead tungstate nanoparticles were successfully synthesized by a sonochemical method for 1 h. XRD patterns showed the body-centered tetragonal structures of PbMoO(4) and PbWO(4), and were in accordance with those of the Simulation and JCPDS software. Calculated lattice parameters are a = b = 5.4233 angstrom and c = 12.1253 angstrom for PbMoO(4), and 5.4570 angstrom and 12.0995 angstrom for PbWO(4). They are in accordance with those of the corresponding JCPDS software. TEM images show that the particles were 29.09 +/- 5.22 nm and 21.05 +/- 2.68 nm for PbMoO(4) and PbWO(4), respectively. Raman and FTIR vibrations were investigated to identify a definite existence of the structures. (C) 2009 Elsevier B.V. All rights reserved. Key Words: SINGLE-CRYSTALS; OPTICAL-PROPERTIES; SPECTRA; POWDERS CURRENT APPLIED PHYSICS Volume: 10 Issue: 1 Pages: 342-345 DOI: 10.1016/j. cap.2009.06.024 Published: JAN 2010. 212 Annotation and in silico localization of the Affymetrix GeneChip Porcine Genome Array Naraballobh Watcharapong2,3, Chomdej Siriwadee3, Murani Eduard2, Wimmers Klaus2 and Ponsuksili Siriluck1 1 Leibniz Institute for Farm Animal Biology, Research Group of Functional Genome, D-18196 Dummerstorf, Germany. 2 Leibniz Institute Farm Animal Biology, Research Unit of Molecular Biology, D-18196 Dummerstorf, Germany. 3 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. Abstract Expression microarrays including the Affymetrix Gene Chip Porcine Genome Arrays are valuable tools for studying genes and functional networks relevant for the expression of complex traits and the responsiveness of the organism to various treatments. An updated annotation and, for the first time, localization on the porcine physical genome map of “Affymetrix GeneChip Porcine Genome Array probe sets” was made through a workflow of 3 pipelines of comparisions addressing various NCBI (National Center for Biotechnology Information) and EnsEMBL (Ensembl project) databases. “BLAST” (Basic Local Alignment Search Tool) comparisons of Affymetrix probe set consensus sequences with the EnsEMBL Sscrofa 9 cDNA database provided 23 799 probe sets with hits. After annotation 19 730 gene symbols were obtained using the data management system BioMart. Comparison of the Affymetrix probe set consensus sequences with the porcine genome sequence (EnsEMBL Sscrofa 9 LatestGP database) revealed 23 298 probe sets with BLAST hits. In the third pipeline in addition to EnsEMBL Sscrofa 9 cDNA and genomic sequence databases also human, mouse and pig NCBI reference sequence RNA databases were interrogated in an integrated approach where also a threshold of bit score >50 or >90% identity over >100 bp was applied in order to filter questionable annotations and localizations. Gene symbols and gene names were queried from HGNC (human genome organization (HUGO) gene nomenclature committee), EASE (EASE: the Expression Analysis Systematic Explorer) and Entrez Gene revealing 20 269 annotated probe sets. 20 467 probe sets were in silico mapped addressing various sources: EnsEMBL Sscrofa 9 LatestGP, preEnsEMBL Sscrofa 8.52 LatestGP, NCBI pig reference sequence RNA and genomic databases and PigQTLdb (Pig Quantitative Trait Locus [QTL] database). Using the new annotation and localization data in functional genomics studies will facilitate improving the understanding of the control of quantitative traits in pigs. Key Words: WATER-HOLDING CAPACITY; CDNA MICROARRAY; EXPRESSION; MUSCLE; GENES; PATHWAYS; RESOURCE; ENSEMBL; CATTLE; PIGS ARCHIV FUR TIERZUCHT-ARCHIVES OF ANIMAL BREEDING Volume: 53 Issue: 2 Pages: 230-238 Published: 2010. 213 Anti-Cancer Compound Screening and Isolation: Coscinium fenestratum, Tinospora crispa and Tinospora cordifolia Tungpradit Rudeewan1,2,3, Sinchaikul Supachok2,3, Phutrakul Suree1, Wongkham Weerah4 and Chen Shui-Tein2,3,5 1 Department of Chemistry, Facutly of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan. 3 Academia Sinica, Genomic Research Center, Taipei 11529, Taiwan. 4 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 5 Institute of Biochemical Science, National Taiwan University, Taipei 10617, Taiwan. Abstract The isolations of active anti-cancer compounds from Coscinium fenestratum, Tinospora crispa and Tinospora cordifolia are rarely reported. In this study, the isolation of anti-cancer compound from these herbs was performed. Cytotoxic activity of the water and methanol extracts of these herbs were evaluated. The methanol extract of C. fenestratum showed the most cytotoxic activity, and contained a major compound of berberine with 3.68%. Cytotoxic screening of berberine against ten cancer cell lines and one normal cell (PMBC) showed the most cytotoxic activity against HL-60 leukemia cells with an IC(50) of 1.41 +/- 0.7 mu g/mL. The selective index (SI) value of berberine against HL-60 cells and PBMC cells were 0.142, indicating the selective cytotoxicity to cancer cells. Interestingly, berberine uptake by cancer cell correlated with cytotoxic activity. In addition, the apoptosis of HL-60 cells against berberine were detected by DAPI staining, and quantitated by Annexin V-FITC staining. Berberine, the effective compound from C. fenestratum, appears to possess anticancer potential. Key words: coscinium fenestratum; tinospora crispa; tinospora cordifolia; cancer; cytotoxicity CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 3 Pages: 476-488 Published: SEP 2010. 214 Antioxidant and Anti-inflammatory Activities of Brown Marine Alga, Padina minor Yamada Peerapornpisal Yuwadee2, Amornlerdpison Doungporn1, Jamjai Utan3, Taesotikul Tawat3, Pongpaibul Yanee4, Nualchareon Mantana5 and Kanjanapothi Duangta3 1 Faculty of Fisheries Technology & Aquatic Resources, Maejo University, Chiang Mai 20290, Thailand. 2 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Phamacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Faculty of Pharmacy, Payap University, Chiang Mai 50000, Thailand. 5 Department of Biology, Faculty of Science & Technology Rajabhat Phuket University, Phuket 83000, Thailand. Abstract Padina minor Yamada is a brown marine alga found in abundance at the coastal area of the Gulf of Thailand and the Andaman Sea. The aqueous extract of P. minor (Aq. P) showed antioxidant activity when tested in superoxide and hydroxyl radical scavenging, metal chelating and reducing capacity assays. Although the concentrations of Aq P. for antioxidant activity were much higher than that of standard drugs when tested in various assays, their maximal effects were approximately the same. Topical application of Aq. P on the rat ear could lessen the ear edema induced by ethyl phenylpropiolate (EPP), suggesting it possesses an anti-inflammatory activity. Interestingly, safety of the Aq. P to be used as a nutraceutical and a cosmeceutical were demonstrated according to the acute toxicity tests in rats and the irritation tests in rabbits, respectively. No signs of acute toxicity and skin irritation were observed. The findings therefore provide the evidence to substantiate the nutraceutical and cosmeceutical potential of P. minor. Key words: Padina minor; antioxidant activity; anti-inflammatory activity; nutraceutical; cosmeceutical CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 3 Pages: 507-516 Published: SEP 2010. 215 AZO/Ag/AZO multilayer films prepared by DC magnetron sputtering for dye-sensitized solar cell application Sutthana S., Hongsith N. and Choopun S. 1 Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 2 Abstract Ag films were deposited on Al-doped ZnO (AZO) films and coated with AZO to fabricate AZO/Ag/AZO multilayer films by DC magnetron sputtering on glass substrates without heating of glass substrates. The best multilayer films have low sheet resistance of 19.8 Omega/Sq and average transmittance values of 61% in visible region. It was found that the highest figure of merit (F(TC)) is 6.9 x 10(-4) Omega(-1). For the dye-sensitized solar cell (DSSC) application, the multilayer films were used as transparent conductive electrode (multilayer films/ZnO + Eosin-Y/ LiI+I(2)/Pt/FTO). The best DSSC based on the multilayer films showed that open circuit voltage (V(oc)) of 0.47 V, short circuit current density (J(sc)) of 2.24 mA/ cm(2), fill factor (FF) of 0.58 and incident photon-to-current conversion efficiency (eta) of 0.61%. It was shown that the AZO/Ag/AZO multilayer films have potential for application in DSSC. (c) 2009 Elsevier B.V. All rights reserved. Key Words: OXIDE THIN-FILMS; INDIUM-TIN-OXIDE; DOPED ZNO FILMS; RESISTANCE; ELECTRODE CURRENT APPLIED PHYSICS Volume: 10 Issue: 3 Pages: 813-816 DOI: 10.1016/ j.cap.2009.09.020 Published: MAY 2010. 216 Botanical notes Maxwell James F. Department of Biology, Faculty of Science, Chiang Mai University Herbarium, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Indigofera caudata Dunn (Leguminosae, Papilionoideae) is noted as a new record for Thailand. Two new combinations in Hedyotis from Oldenlandia (Rubiaceae) are made, viz. Hedyotis krewanhensis (Pierre ex Pit.) Maxw. and Hedyotis chevalieri (Pit.) Maxw. Key words: Indigofera caudata Dunn; Oldenlandia krewanhensis Pierre ex Pit.; Oldenlandia chevalieri Pit.; Hedyotis krewanhensis (Pierre ex Pit.) Maxw.; Hedyotis chevalieri (Pit.) Maxw. MAEJO INTERNATIONAL JOURNAL OF SCIENCE AND TECHNOLOGY Volume: 4 Issue: 3 Pages: 547-551 Published: SEP-DEC 2010. 217 Calculation of Weighted Averages Approach for the Estimation of Ping Tolerance Values Silalom Somyot1,2, Carter James L.3 and Chantaramongkol Porntip1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Queen Sirikit Botanic Garden, Chiang Mai 50180, Thailand. 3 US Geological Survey, Menlo Park, CA 94025 USA. Abstract A biotic index was created and proposed as a tool to assess water quality in the Upper Mae Ping sub-watersheds. The Ping biotic index was calculated by utilizing Ping tolerance values. This paper presents the calculation of Ping tolerance values of the collected macroinvertebrates. Ping tolerance values were estimated by a weighted averages approach based on the abundance of macroinvertebrates and six chemical constituents that include conductivity, dissolved oxygen, biochemical oxygen demand, ammonia nitrogen, nitrate nitrogen and orthophosphate. Ping tolerance values range from 0 to 10. Macroinvertebrates assigned a 0 are very sensitive to organic pollution while macroinvertebrates assigned 10 are highly tolerant to pollution. Key Words: BIOTIC INDEX CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 1 Pages: 150-158 Published: JAN 2010. 218 Can leaf degrading enzymes provide evidence that endophytic fungi becoming saprobes? Promputtha Itthayakorn1, Hyde Kevin D.2, McKenzie Eric H. C.3, Peberdy John F.4 and Lumyong Saisamorn5 1 School Cosmetic Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand. School Science, Muang, Mae Fah Luang University, Chiang Rai 57100, Thailand. 3 Landcare Research, Auckland, New Zealand. 4 University Nottingham, School Business, Institute Enterprise & Innovation, Nottingham NG8 1BB, England. 5 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract The diversity of endophytic and saprobic fungi from Magnolia liliifera leaves were observed and analyzed to establish relationships. Nine endophytes were morphologically and phylogenetically similar to the saprobes; Colletotrichum gloeosporioides, Colletotrichum sp. 2, Corynespora cassiicola, Fusarium sp. 1, Guignardia mangiferae, Leptosphaeria sp., Phomopsis sp. 2, Phomopsis sp. 6, and Phomopsis sp. 10. The endophytes were found to produce the same degrading enzymes as their saprobic counterparts. The isoform of beta-mannanase produced from each of endophyte and saprobe counterparts were similar. Fungal succession and enzyme production patterns during leaf decomposition were correlated. The occurrence of saprobes was found to be related to the enzymes that the fungi produce. The study provides further compelling evidence that endophytes can switch lifestyle to saprobes. Key Words: PUI NATIONAL-PARK; TEMPERATE DECIDUOUS FOREST; SENESCENT LEAVES; PECTINOLYTIC ACTIVITY; WOOD-DECAY; ABIES-ALBA; LITTER; SUCCESSION; DIVERSITY; THAILAND FUNGAL DIVERSITY Volume: 41 Issue: 1 Pages: 89-99 DOI: 10.1007/s13225-010-0024-6 Published: MAR 2010. 219 Carboxymethyl Cellulose-Assisted Hydrothermal Synthesis of PbS with Nano- and Micro-Crystals Thongtem Titipun1, Kaowphong Sulawan2 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Meterial Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract PbS with nano- and micro-crystals was hydrothermally synthesized from Pb(NO(3))(2) and thiosemicarbazide using carboxymethyl cellulose (CMC) as a template at 140, 180 and 200 degrees C for 12 h. CMC, NaOH and hydrothermal temperatures have the influence on the product morphologies characterized using a scanning electron microscope (SEM) and a transmission electron microscope (TEM). PbS (cubic) composing of Pb and S was detected using an X-ray diffractometer (XRD), a selected area electron diffraction (SAED) technique and an energy dispersive X-ray (EDX) analyzer. The interpreted patterns are in accordance with those of the simulations. Raman spectrometer revealed the presence of the vibration modes at 136, 278, 432, 602 and 967 cm(-1). Emission spectra of the products were detected at 384-388 nm using a photoluminescence (PL) spectrometer. Key Words: SHAPE-CONTROLLED SYNTHESIS; RAMAN-SPECTRA; NANOCUBES; NANOSTRUCTURES; MICROCRYSTALS; DENDRITES; EVOLUTION; NANOBELTS; GROWTH JOURNAL OF NANOSCIENCE ANDNANOTECHNOLOGY Volume: 10 Issue: 4 Pages: 2853-2857 DOI: 10.1166/jnn.2010.1375 Published: APR 2010. 220 Changes in ferroelectric properties of lead indium niobate-lead titanate ceramics under compressive stress applied perpendicular to an electric field Unruan Muangjai1, Wongsaenmai Supattra2, Ngamjarurojana Athipong1, Laosiritaworn Yongyut1, Ananta Supon1, Guo Ruyan3, Bhalla Amar3 and Yimnirun Rattikorn4 1 Department of Physics & Material Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand. 3 Department of Electrical & Computer Engineering, College of Engineering, University of Texas San Antonio, San Antonio, TX 78249 USA. 4 Institute of Science, School of Physics, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Abstract The influences of perpendicular compressive stress on the ferroelectric properties of (1 - x)Pb(In(1/2)Nb(1/2))O(3)-xPbTiO(3) (x = 0.1-0.4) ceramics were investigated in this study. In general, with increasing compressive stress the coercive field and the dissipation energy of the ceramics were seen to increase. The stress-induced changes in the polarization, however, depended significantly on ceramic compositions. The observations were mainly interpreted in terms of competing influence of the domain switching through non-180 degrees domain walls, clamping of domain walls, and the stress-induced decrease in the switchable part of spontaneous polarization. (C) 2009 Elsevier B.V. All rights reserved. Key Words: UNIAXIAL-STRESS; DIELECTRIC-PROPERTIES; ELECTROMECHANICAL PROPERTIES; PIEZOELECTRIC PROPERTIES; SINGLE-CRYSTALS; SOFT PZT; RELAXOR; PHASE; DEPENDENCE; PRESSURE PHYSICS LETTERS A Volume: 374 Issue: 9 Pages: 1147-1153 DOI:10.1016/j.physleta.2009.12.064 Published: FEB 15 2010. 221 Changes in the 2DE protein profiles of chilli pepper (Capsicum annuum) leaves in response to Fusarium oxysporum infection Wongpia Aphinya1 and Lomthaisong Khemika2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. Abstract Wilt disease caused by Fusarium oxysporum f. sp. capsici is a major problem of chilli pepper production worldwide that calls for a better understanding of defensive mechanisms in the chilli plant. We used a proteomic technique to investigate protein responses of chilli pepper to E oxysporum f. sp. capsici. Two cultivars of resistant (Mae Ping 80) and susceptible (Long Chilli 455) plants were cultured in vitro. Chilli plants at 6-week growth were then infected with a suspension of F oxysporum f. sp. capsici or distilled water used as a control. After 48 h of infection, proteins were extracted and analysed using 2DE to identify the responsive proteins. At least 9 spots were differentially expressed in the resistant cultivar (5 increasing, 4 decreasing) and 1 supplementary; while 15 increasing, 11 decreasing, and 11 supplementary protein spots were found in the susceptible cultivar. These proteins were then identified by MALDI-TOF MS combined with bioinformatics methods. Some of the induced proteins e.g., NADPH HC toxin reductase, serine/ threonine protein kinase, and 1-aminocyclopropane-1-carboxylate synthase 3 are involved in plant defence mechanism. In order to determine the Fusarium wilt protective proteins in chilli plant, the protein patterns of healthy resistance were compared with those of susceptible cultivars. Interestingly, resistance showed higher expression of proteins related to ROS detoxification. Moreover, the ability of chilli plant to resist Fusarium wilt disease was related to the expression of non-inducible immunity 1 protein. Key Words: SYSTEMIC ACQUIRED-RESISTANCE; PLANT-DISEASE RESISTANCE; PROGRAMMED CELL-DEATH; PROTEOMIC ANALYSIS; SUPEROXIDE-DISMUTASE; DEFENSE PROTEINS; WHEAT SPIKES; RICE; GENE; ARABIDOPSIS SCIENCEASIA Volume: 36 Issue: 4 Pages: 259-270 DOI: 10.2306/scienceasia 15131874.2010.36.259 Published: DEC 2010. 222 Characterization of AgBiS(2) nanostructured flowers produced by solvothermal reaction Thongtem Titipun1, Tipcompor Narongrit1 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Material Sceince, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract AgBiS(2) nanostructured flowers were produced from CH(3)COOAg, Bi(NO(3)) (3 center dot)5H(2)O and thiosemicarbazide (NH(2)CSNHNH(2)) using different solvents [ethylene glycol (EG). water (H(2)O), polyethylene glycol with molecular weight of 200 (PEG200), and propylene glycol (PG)] in Teflon-lined stainless steel autoclaves. The phase and purity were detected using X-ray diffraction (XRD), controlled by the solvents. The product was purified AgBiS2 produced by the 200 degrees C and 24 h reaction in EG, corresponding to selected area electron diffraction (SAED) and simulation patterns. Scanning and transmission electron microscopies (SEM and TEM) revealed the formation of nanostructured flowers - enlarged by the increase in the lengths of time and temperature. Their photoluminescence (PL) emissions were detected at the same wavelength of 382 nm (3.24 eV), although they were produced under different conditions. (C) 2010 Elsevier B.V. All rights reserved Key Words: POLYOL ROUTE; NANOWIRES; NANORODS MATERIALS LETTERS Volume: 64 Issue: 6 Pages: 755-758 DOI: 10.1016/j.matlet.2010.01.003 Published: MAR 31 2010. 223 Characterization of Ba(0.77)Sr(0.23)TiO(3) powder prepared from an oxalate co-precipitation and an impregnation method Pookmanee Pusit1 and Phanichphant Sukon2,3 1 Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand. Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 NANOTEC Center of Excellence, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract Barium titanate (BaTiO(3)) powder was prepared from an oxalate co-precipitation method with the starting precursors of barium chloride dihydrate and potassium titanium oxalate dihydrate with mole ratio of 1: 1. Precipitated powder was obtained after adding oxalic acid until the pH of the final solution was 2. The precipitated powder was milled and calcined at 700 degrees C for 2 h. Sr-doped barium titanate (BaSrTiO(3)) powder was prepared by an impregnation method. Barium titanate calcined at 700 degrees C for 2 h was mixed with 2 and 4 mole % of Sr from strontium chloride hexahydrate. The mixed powder was calcined at 900 degrees C for 2 h. The phase of Ba(0.77)Sr(0.23)TiO(3) powder was studied by X-ray diffraction (XRD) and found to have a tetragonal structure after calcination at 900 degrees C for 2 h. The morphology and chemical composition of Ba(0.77) Sr(0.23)TiO(3) powder were investigated by a scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS). The particle size of Ba(0.77) Sr(0.23)TiO(3) powder was in the range of 0.2-0.3 mu m with an irregular shape. The elemental composition of barium, strontium, titanium and oxygen showed the characteristic X-ray energy values. Key words: Ba(0.77)Sr(0.23)TiO(3); Oxalate co-precipitation; Impregnation JOURNAL OF CERAMIC PROCESSING RESEARCH Volume: 11 Issue: 3 Pages: 384-387 Published: JUN 2010. 224 Characterization of Bi(2)S(3) with different morphologies synthesized using microwave radiation Thongtem Titipun1, Phuruangrat Anukorn2, Wannapop Surangkana2 and Thongtem Somcha2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Material Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Bi(2)S(3) with different morphologies (nanoparticles, nanorods and nanotubes) was synthesized using bismuth nitrate pentahydrate (Bi(NO(3))(3)center dot 5H(2) O) and two kinds of sulfur sources (CH(3)CSNH(2) and NH(2)CSNH(2)) ill different solvents (water, ethylene glycol and propylene glycol) via a microwave radiation method at 180 W for 20 min. X-ray powder diffraction (XRD). scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicated that all of the products are orthorhombic Bi(2)S(3) phase of nanoparticles, nanorods and nanotubes, influenced by the sulfur sources and solvents. Formation mechanisms of the products with different morphologies are also proposed. (C) 2009 Elsevier B.V. All rights reserved. Key Words: NANORODS MATERIALS LETTERS Volume: 64 Issue: 2 Pages: 122-124 DOI: 10.1016/j.matlet.2009.10.006 Published: JAN 31 2010. 225 Characterization of copper sulfide nanostructured spheres and nanotubes synthesized by microwave-assisted solvothermal method Thongtem Titipun1, Phuruangrat Anukorn2 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Material Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Copper Sulfide nanostructured spheres and nanotubes were successfully synthesized. using a microwave-assisted solvothermal method, by the decomposition of [Cu(CH(3)CSNH(2))(2)]Cl(2) complexes. formed by the reaction of CuCl(2) center dot 2H(2)O and CH(3)CSNH(2) in ethylene glycol at different pH values, and identified by CHNS/O and FTIR analyses. The decrease in bonding energy of N-H revealed the coordination of copper ions and thioacetamide molecules. It was specified that nitrogen atoms of thioacetamide molecules were used to form Cu-thioacetamide complexes. XRD, SEM, TEM and SAED analyses show that the products were hexagonal CuS spheres in an extremely low pH solution, and hexagonal CuS nanotubes at a pH 13. Their Raman spectra show sharp peaks at 473 cm(-1), identified as the S-S stretching mode Of S(2) ions at the 4e sites. (C) 2009 Elsevier B.V. All rights reserved. Key Words: CUS; NANOBELTS; POWDERS MATERIALS LETTERS Volume: 64 Issue: 2 Pages: 136-139 DOI:10.1016/j.matlet.2009.10.021 Published: JAN 31 2010. 226 Characterization of micro-crystalline lead tungstate with different morphologies produced by the sonochemical process Kaowphong Sulawan1, Thongtem Titipun2 and Thongtem Somchai1 1 Department of Physics & Material Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Micro-crystalline PbWO(4) was sonochemically produced using Pb(CH(3) COO)(2) center dot 3H(2)O and Na(2)WO(4) center dot 2H(2)O in aqueous solutions with and without anionic surfactant (sodium dodecyl sulphate or sodium lauryl sulphate). The product morphologies were influenced by the pH values and the surfactant. Micro-crystalline PbWO(4) composing of Pb, W and O, and W-O stretching vibration band of WO(4) tetrahedrons were detected using XRD, SEM, EDX, TEM, SAED and FTIR. Photoluminescent intensities, caused by the electronic transition from (1)T(2) to (1)A(1) states, shown to increase with the increase in the pH values of the solutions. Key Words: MICROWAVE-ASSISTED SYNTHESIS; PBWO4 NANO; SCHEELITE; ROUTE; LUMINESCENCE; CRYSTALS; MINERALS; POWDERS; CA; SR RUSSIAN JOURNAL OF INORGANIC CHEMISTRY Volume: 55 Issue: 4 Pages: 577-582 DOI: 10.1134/S0036023610040145 Published: APR 2010. 227 CHARACTERIZATION OF MULTIPOD CADMIUM SULFIDE NANOSTRUCTURES SYNTHESIZED BY AMINETHERMAL METHOD Phuruangrat A.1,3, Thongtem T.2,3 and Thongtem S.1,3 1 Department of Physics & Material Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Material Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Multipod (monopod, bipod, tripod and tetrapod) CdS nanostructures have been successfully synthesized under aminethermal method at 200 degrees C for 10 h without using any templates and surfactants. The product was characterized by X-ray diffraction (XRD), Raman spectroscopy, fast Fourier transformation (FFT), transmission electron microscopy (TEM), and high resolution TEM (HRTEM). XRD pattern shows wurtzite CdS structure. Its Raman spectrum respectively shows the LO(1) and LO(2) of A(1) vibration mode at 300 and 601 cm(-1), which are polarized in the x-z face, and strongly couple to the excitons along the c axis. The structures of multipod CdS were characterized using TEM and HRTEM. Key words: Multipod CdS nanostructures; Aminethermal method CHALCOGENIDE LETTERS Volume: 7 Issue: 10 Pages: 605-608 Published: OCT 2010. 228 Characterization of nanostructured ZnO produced by microwave irradiation Thongtem Titipun1, Phuruangrat Anukorn2 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Material Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Different molar ratios of Zn(NO(3))(2) to NaOH were dissolved in de-ionized water, mixed to form solutions with different pH values and heated using 180 W microwave power (80 degrees C) in ambient atmosphere for 20 min. Wurtzite ZnO nanostructure was detected using an X-ray diffractometer (XRD) and a selected area electron diffraction (SAED) technique. The patterns were in accordance with those of the simulation. Scanning and transmission electron microscopes (SEM and TEM) revealed their nanostructures with different morphologies controlled by molar ratios of the starting agents as well as pH values of the solutions. High resolution transmission electron microscopic (HRTEM) technique shows that the crystallographic planes are aligned in lattice array. Seven different Raman wavenumbers at 334, 378,410, 440, 541, 575 and 660 cm(-1) were used to specify that the products were wurtzite structured ZnO. Photoluminescence (PL) spectra show their emission peaks at 385-394 nm due to the recombination process of free excitons. (C) 2009 Elsevier Ltd and Techna Group S.r.l. All rights reserved. Key Words: LOW-TEMPERATURE SYNTHESIS; HYDROTHERMAL PROCESS; LARGE-SCALE; FLOWERS; GROWTH; MICROSPHERES; ROUTE CERAMICS INTERNATIONAL Volume: 36 Issue: 1 Pages: 257-262 DOI: 10.1016/j.ceramint.2009.07.027 Published: JAN 2010. 229 Characterization of SrCO(3) and BaCO(3) nanoparticles synthesized by sonochemical method Thongtem Titipun1, Tipcompor Narongrit1, Phuruangrat Anukorn2 and Thongtem Somchai2 1 Chiang Mai University, Faculty of Science, Department of Chemistry, PERCH CIC, Chiang Mai 50200, Thailand. 2 Chiang Mai University, Faculty of Science, Department of Physics & Material Science, Chiang Mai 50200, Thailand. Abstract SrCO(3) and BaCO(3) nanoparticles were synthesized using Sr(NO(3))(2) or Ba(NO(3))(2) and Na(2)CO(3) as starting materials in ethylene glycol by ultrasonic irradiation at 80 degrees C for 1-5 h. Their phases, vibration modes and morphologies were characterized using X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, selected area electron diffraction (SAED) and transmission electron microscopy (TEM). These products were found to be orthorhombic SrCO(3) and BaCO(3) nanoparticles with 20-50 nm and 40-100 nm ranges, respectively. Asymmetric stretching, symmetric stretching, and out of plane and in plane bending vibrations of CO(3)(2-) complexes were also detected. (C) 2009 Elsevier B.V. All rights reserved. Key Words: NANOSTRUCTURES MATERIALS LETTERS Volume: 64 Issue: 4 Pages: 510-512 DOI: 10.1016/j.matlet.2009.11.060 Published: FEB 28 2010. 230 Clubionidae from Laos and Thailand (Arachnida: Araneae) Jaeger Peter1 and Dankittipakul Pakawin2 1 Senckenberg Research Institute, D-60325 Frankfurt, Germany. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract Clubionidae collected in Laos and Thailand were investigated. Seven new species are described: Clubiona kai spec. nov. (male: Laos, Luang Prabang Prov.), Clubiona lala spec. nov. (female: Laos, Luang Nam Tha Prov.), Clubiona maipaispec. nov. (male: Thailand, Mae Hong Son Prov.), Clubiona kuu spec. nov. (male: Laos, Luang Prabang Prov.), Clubiona vukomi spec. nov. (male: Laos, Luang Nam Tha Prov.), Malamatidia zu spec. nov. (male: Laos, Luang Prabang Prov.) and Malamatidia christae spec. nov. (female: Laos, Luang Nam Tha Prov.). The genus Malamatidia, previously known from Sumatra, Sarawak, the Malaysian peninsular, Kalimantan, and Sulawesi is recorded from Laos (Luang Nam Tha Province) as northernmost distribution locality and is re-diagnosed. Cheiracanthium insulanum (Thorell, 1878), Cheiracanthium unicum Bosenberg and Strand, 1906, Clubiona abnormis Dankittipakul, 2008, Clubiona filicata O. Pickard-Cambridge, 1874, Clubiona melanothele Thorell, 1895 are recorded for the first time from Laos. Cheiracanthium insulanum, Clubiona abnormis, and Clubiona melanothele are additionally illustrated. A map with all records is provided. Key Words: SPIDERS ZOOTAXA Issue: 2730 Pages: 23-43 Published: DEC 24 2010. 231 Comparison of different methods for determination of Pt surface site concentrations for supported Pt electrocatalysts Punyawudho K.2, Blom D. A.3, Van Zee J. W. and Monnier J. R.1 1 Department of Chemistry Engineering, Swearingen Engineering Center, College of Engineering & Computing Science, University South Carolina, Columbia, SC 29208 USA. 2 Department of Industrail Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. 3 Electron Microscopy Center, University of South Carolina, Columbia, SC 29208 USA. Abstract Platinum surface atom (or site) concentrations for a series of commercially available 10, 20, and 40 wt% Pt/C electrocatalysts have been determined using Xray diffraction (XRD), high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM), selective chemisorption, and cyclic voltammerry (CV) methods. Each method of analysis was repeated for a sufficient number of times to determine reproducibility and standard deviation limits. Comparison of the results shows that XRD and STEM methods give Pt surface site concentrations much higher than for chemisorption analysis due to assumptions regarding Pt particle shapes and particle size distributions. The results from CV analysis agree reasonably well with those from chemisorption if the sample amounts and methods of sample deposition preceding CV analysis can be well-controlled and there is no loss of surface exposure by the Nation over-layer. Because both chemisorption and CV analyses more directly measure actual site concentrations with fewer assumptions, these methods should be considered superior to XRD and STEM analyses. Further, since chemisorption uses substantially larger sample sizes (up to 0.25 g) compared to CV (<0.01 g), reliability of chemisorption data is much more reliable and should be considered as the metric for surface Pt site determination. (C) 2010 Elsevier Ltd. All rights reserved. Key words: Pt surface sites; Pt/C electrocatalysts; Cyclic voltammetry; Chemisorption; X-ray line broadening ELECTROCHIMICA ACTA Volume: 55 Issue: 19 Pages: 5349-5356 DOI: 10.1016/j.electacta.2010.04.062 Published: JUL 30 2010. 232 Comparison of gamma-aminobutyric acid production in Thai rice grains Jannoey Panatda1,2, Niamsup Hataichanoke1,2, Lumyong Saisamorn3, Suzuki Toshisada4, Katayama Takeshi4 and Chairote Griangsak1,2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Department of Biochemistry & Food Science, Faculty of Agriculture, Kagawa University, Kagawa, Japan. Abstract Gamma-aminobutyric acid (GABA) has many pharmacological functions including being a major inhibitory neurotransmitter. Two comparative methods for GABA production in rice grains as main food source in Thailand were investigated in this study. Fermentation and germination method were separately carried out using seven selected local grain cultivars in northern Thailand. Red yeast rice, obtained from the fermentation method, gave the higher GABA concentration than the germinated rice produced from the germination method in most rice cultivars. The highest GABA concentration was 28.37 mg/g at 3 weeks fermentation time of glutinous rice, O. sativa L. cv. Sanpatong 1 cultivars, while germinated rice from glutinous rice; O. sativa L. cv. Korkor6 (RD6) cultivars contained the highest GABA concentration of 3.86 mg/g. These results provide information for the basis of an appropriate method for GABA production. The fermentation produced higher GABA concentration but required longer production period and red yeast rice was obtained as product. On the other hand, the germination method yielded rice grains with lower GABA but in more suitable form for consumption. Both methods are considered to be economical and efficient methods to increase GABA in rice grains, providing alternative products with higher nutritional values. Key Words: GERMINATED BROWN RICE; SOLID-STATE FERMENTATION; GLUTAMATE-DECARBOXYLASE; ANAEROBIC ACCUMULATION; LACTOBACILLUS-BREVIS; MONASCUS-PURPUREUS; MONACOLIN-K; GABA; CALMODULIN; SEEDLINGS WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY Volume: 26 Issue: 2 Pages: 257-263 DOI: 10.1007/s11274-009-0168-2 Published: FEB 2010. 233 Complexity of terms, superpositions, and generalized hypersubstitutions Puninagool Wattapong and Leeratanavalee Sorasak Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In this paper, we consider the four useful measurements of the complexity of a term, called the maximum depth, the minimum depth, the variable count, and the operation count. We construct a formula for the complexity of the superposition S(m)(s, t(1), ..., t(m)) in terms of complexity of the inputs s, t(1), ..., t(m) for each of these measurements. We also obtain formulas for the complexity of (sigma) over cap [t] in terms of the complexity where t is a compound term and sigma is a generalized hypersubstitution. We apply these formulas to the theory of M-strongly solid varieties, examining the k-normalization chains of a variety with respect to these complexity measurements. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved. COMPUTERS & MATHEMATICS WITH APPLICATIONS Volume: 59 Issue: 2 Pages: 10381045 DOI:10.1016/j.camwa.2009.06.033 Published: JAN 2010. 234 Comprehensive Two-Dimensional Gas Chromatography-Mass Spectrometry Analysis of Volatile Constituents in Thai Vetiver Root Oils Obtained by Using Different Extraction Methods Pripdeevech Patcharee1, Wongpornchai Sugunya1 and Marriott Philip J.2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Applied Science, Australian Center for Research on Separat Science, RMIT University, Melbourne, Vic 3001, Australia. Abstract Introduction: Vetiver root oil is known as one of the finest fixatives used in perfumery. This highly complex oil contains more than 200 components, which are mainly sesquiterpene hydrocarbons and their oxygenated derivatives. Since conventional GC-MS has limitation in terms of separation efficiency, the comprehensive two-dimensional GC-MS (GC x GC-MS) was proposed in this study as an alternative technique for the analysis of vetiver oil constituents. Objective: To evaluate efficiency of the hyphenated GC x GC-MS technique in terms of separation power and sensitivity prior to identification and quantitation of the volatile constituents in a variety of vetiver root oil samples. Methodology: Dried roots of Vetiveria zizonioides were subjected to extraction using various conditions of four different methods; simultaneous steam distillation, supercritical fluid, microwaveassisted, and Soxhlet extraction. Volatile components in all vetiver root oil samples were separated and identified by GC-MS and GC X GC-MS. The relative contents of volatile constituents in each vetiver oil sample were calculated using the peak volume normalization method. Results: Different techniques of extraction had diverse effects on yield, physical and chemical properties of the vetiver root oils obtained. Overall, 64 volatile constituents were identified by GC-MS. Among the 245 well-resolved individual components obtained by GC x GC-MS, the additional identification of 43 more volatiles was achieved. Conclusion: In comparison with GC-MS, GC x GC-MS showed greater ability to differentiate the quality of essential oils obtained from diverse extraction conditions in terms of their volatile compositions and contents. Copyright (C) 2009 John Wiley & Sons, Ltd. Key Words: SUPERCRITICAL CARBON-DIOXIDE; GRASS; RESOLUTION; SOXHLET PHYTOCHEMICAL ANALYSIS Volume: 21 Issue: 2 Pages: 163-173 DOI: 10.1002/pca.1173 Published: MAR-APR 2010. 235 Compressive strength and microstructure of carbon nanotubes-fly ash cement composites Chaipanich Arnon, Nochaiya Thanongsak, Wongkeo Watcharapong and Torkittikul Pincha Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In this work, carbon nanotubes of 0.5 and 1% by weight were added for the first time in a fly ash cement system to produce carbon nanotubes-fly ash composites in the form of pastes and mortars. Compressive strengths of the composites were then investigated. It was found that the use of carbon nanotubes resulted in higher strength of fly ash mortars. The highest strength obtained for 20% fly ash cement mortars was found at 1% carbon nanotubes where the compressive strength at 28 days was 51.8 MPa. This benefit can clearly be seen in fly ash cement with fly ash of 20% where the importance of the addition of carbon nanotubes means that the relative strength to that of Portland cement became almost 100% at 28 days. In addition, scanning electron micrographs also showed that good interaction between carbon nanotubes and the fly ash cement matrix is seen with carbon nanotubes acting as a filler resulting in a denser microstructure and higher strength when compared to the reference fly ash mix without CNTs. (C) 2009 Elsevier B.V. All rights reserved. Key Words: REINFORCED POLYMER COMPOSITES; MECHANICAL-PROPERTIES; CORROSION-RESISTANCE; FRACTURE-TOUGHNESS; MATRIX COMPOSITES; SILICA FUME; BEHAVIOR; FABRICATION; CONCRETE; EPOXY MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING Volume: 527 Issue: 4-5 Pages: 1063-1067 DOI: 10.1016/j. msea.2009.09.039 Published: FEB 15 2010. 236 Compressive strength, microstructure and thermal analysis of autoclaved and air cured structural lightweight concrete made with coal bottom ash and silica fume Wongkeo Watcharapong and Chaipanich Arnon Cement & Concrete Research Laboratory, Construction & Materials Research Unit, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract This research investigated the compressive strength, microstructure and thermal analysis of autoclaved and air cured structural lightweight concrete made with coal bottom ash and silica fume. The results show that bottom ash lightweight concrete autoclaved for 6 h gives compressive strength similar to the bottom ash lightweight concrete air cured for 28 days and found that the compressive strength of both bottom ash lightweight concrete increased when silica fume was added to the mix. The highest compressive strength obtained for all mixes was found when coal bottom ash was used at 20% with the addition of silica fume at 5% and that this strength value is significantly higher than that of Portland cement control. The thermal conductivity of all bottom ash lightweight concrete at 28 days and those autoclaved for 6 h were found to be slightly higher than that of Portland cement control concrete. Air cued hydration products such as ettringite, calcium silicate hydrate and gehlenite hydrate were detected using thermogravimetric analysis. The tobermorite phase detected in autoclaved bottom ash concrete with silica fume was found to give denser microstructure than the fibrous-like C-S-H phases detected in Portland cement control concrete. (C) 2010 Elsevier B.V. All rights reserved. Key words: Compressive strength; Thermal conductivity; Microstructure; TGA; Bottom ash MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING Volume: 527 Issue: 16-17 Pages: 3676-3684 DOI: 10.1016/j.msea.2010.01.089 Published: JUN 25 2010. 237 Controlled Gd(2)O(3) nanorods and nanotubes by the annealing of Gd(OH)(3) nanorod and nanotube precursors and self-templates produced by a microwave-assisted hydrothermal process Thongtem Titipun1, Phuruangrat Anukorn2,3, Ham Dong Jin3, Lee Jae Sung3 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Pohang University Science & Technology, Ecofriendly Catalysis & Energy Loboratory NRL, Department of Chemistry Engineering, School Environmental Science & Environmental, Pohang 790784, South Korea. Abstract One dimensional Gd(OH)(3) nanorods and nanotubes were successfully produced through a microwave-assisted hydrothermal process of Gd(NO(3))(3) solutions at pH 10, adjusted using 3 M NaOH and NH(4)OH as buffer solutions. Subsequently, the Gd(OH)3 nanorods and nanotubes, functioned as both the precursors and self-templates, were annealed at 450 degrees C for 2 h to form the respective Gd(2)O(3) nanorods and nanotubes whilst retaining their shapes. X-Ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and selected area electron diffraction (SAED) were used to characterize these Gd(OH) (3) and Gd(2)O(3) nanorods and nanotubes, of which the formation mechanisms were also proposed. Key Words: HYDROXIDE NANORODS; OPTICAL-PROPERTIES; NANOPARTICLES; PHOSPHOR; NANOCRYSTALS; NANOWIRES CRYSTENGCOMM Volume: 12 Issue: 10 Pages: 2962-2966 DOI: 10.1039/b925097b Published: 2010. 238 Convergence Analysis for a System of Equilibrium Problems and a Countable Family of Relatively Quasi-Nonexpansive Mappings in Banach Spaces Cholamjiak Prasit and Suantai Suthep Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract We introduce a new hybrid iterative scheme for finding a common element in the solutions set of a system of equilibrium problems and the common fixed points set of an infinitely countable family of relatively quasi-nonexpansive mappings in the framework of Banach spaces. We prove the strong convergence theorem by the shrinking projection method. In addition, the results obtained in this paper can be applied to a system of variational inequality problems and to a system of convex minimization problems in a Banach space. Key Words: WEAK-CONVERGENCE; THEOREMS; OPERATORS ABSTRACT AND APPLIED ANALYSIS Article Number: 141376 DOI: 10.1155/2010/141376 Published: 2010. 239 Cultivation of Spirulina platensis using Anaerobically Swine Wastewater Treatment Effluent Cheunbarn Siraporn1 and Peerapornpisal Yuwadee2 1 Faculty of Science, Maejo University, Chiang Mai 50290, Thailand. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50290, Thailand. 2 Abstract In this study, Spirulina platensis was cultivated by using effluent from anaerobically treated swine wastewater at concentration of 0, 10, 20, 40, 60, 80 and 100% dilution. Results showed that the most suitable concentration for maximum growth of S. platensis was 10% dilution with NaHCO(3) and NaNO(3) at 8.0 g L(-1) and 1.5 g L(-1), respectively. From a batch culture with 10% dilute concentration and added nutrients, highest wastewater treatment efficiency occurred after 12 days retention time when maximum removal efficiency was measured at 23, 45, 49, 92 and 67% for COD, BOD, NO(3)(-), NH(4)(+) and PO(4)(3-), respectively. Maximum growth of S. platensis was measured by 17.8x10(4) cells mL(-1) and value of OD(560) at 1.09 with 55.88 % protein content by dry weight. This suggested that S. platensis could be applied for use as a food nutrient in the farm that could decrease farm cost. (C) 2010 Friends Science Publishers Key words: Spirulina platensis; Swine wastewater; Treatment efficiency; Nutrient INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY Volume: 12 Issue: 4 Pages: 586-590 Published: JUL 2010. 240 Defect-free Poly(9,9-bis(2-ethylhexyl)fluorene-2,7-vinylene) for Polymer Light-Emitting Diode (PLED) Devices Auragudom Piched1,2, Tangonan Andrew A.3, Namboothiry Manoj A. G.4, Carroll David L.4, Advincula Rigoberto C.3, Phanichphant Sukon1,2 and Lee T. Randall3 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Faculty of Science, Center for Innovation in Chemistry PERCH CIC, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, University of Houston, Houston, TX 77204 USA. 4 Department of Physics, Center for Nanotechnology & Molecular Materials, Wake Forest University, Winston Salem, NC 27109 USA. Abstract The pi-conjugated light-emitting polymer poly(9,9-bis(2-ethylhexyl)fluorene2,7-vinylene) (PEHFV), was synthesized in defect-free form via Horner-Emmons coupling. The structure and properties of the polymer were characterized by (1) H NMR, (13)C NMR, UV-vis, photoluminescence (PL), and electroluminescence (EL) spectroscopies as well as gel permeation chromatography (GPC) and thermogravimetric analysis (TGA). The weight-average molecular weight (M(w)) and polydispersity of the PEHFV were 34,000 g/mol and 2.3, respectively. The UV-vis spectra showed absorption maxima at 425 and 452 nm, and the PL emission spectra showed a maximum at 505 nm with a shoulder at 541 nm. The polymer was soluble in common organic solvents and easily spin-coated on indium-tin oxide (ITO)-coated glass substrates. A double-layer light-emitting device with an ITO/PEDOT:PSS/PFV/Al configuration was fabricated. The turn-on voltage for the PEHFV device was observed at 3.0 V. Key Words: POLYFLUORENE DERIVATIVES; ELECTROLUMINESCENT DIODES; PPV DERIVATIVES; POLY(9,9-DI-N-OCTYLFLUORENYL-2,7-VINYLENE); COPOLYMERS; EFFICIENCY JOURNAL OF POLYMER RESEARCH Volume: 17 Issue: 3 Pages: 347-353 DOI: 10.1007/ s10965-009-9321-8 Published: MAY 2010. 241 Demiclosed Principle for Asymptotically Nonexpansive Mappings in CAT(0) Spaces Nanjaras B. and Panyanak B. Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract We prove the demiclosed principle for asymptotically nonexpansive mappings in CAT(0) spaces. As a consequence, we obtain a Delta-convergence theorem of the Krasnosel’skii-Mann iteration for asymptotically nonexpansive mappings in this setting. Our results extend and improve many results in the literature. Key Words: FIXED-POINT SETS; BANACH-SPACES; INVARIANT APPROXIMATIONS; METRIC-SPACES; CONVERGENCE; BEHAVIOR; CONTRACTIONS; THEOREMS; CAT(0)-SPACES; SEMIGROUPS FIXED POINT THEORY AND APPLICATIONS Article Number: 268780 DOI: 10.1155/2010/268780 Published: 2010. 242 Determination of nitrofurans residues in animal feeds by flow injection chemiluminescence procedure Thongsrisomboon Pawinee1, Liawruangrath Boonsom1, Liawruangrath Saisunee2 and Satienperakul Sakchai3 1 Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand. Abstract A simple flow injection chemiluminescence (Fl-CL) method was developed for the rapid and sensitive determination of nitrofurans, including furazolidone, nitrofurantoin and nitrofurazone, in animal feeds based on its chemiluminescence induced by potassium permanganate in sulphuric acid medium. The method involves the injection of nitrofuran samples or standards into H(2)SO(4) carrier stream, which then merges at a T-piece with a reagent stream consisting of KMnO(4) in the H(2)SO(4) carrier solution. The elicited chemiluminescence intensity of the resulting reaction mixture was measured by photomultiplier tube operated at a voltage of 950 V. Optimum CL signals were given using 2.5 x 10(-5) mol L(-1) potassium permanganate in 0.1 mol L(-1) sulphuric acid as an oxidant stream and a carrier stream of 0.1 mol L(-1) sulphuric acid with a total flow rate of 7.0 mL min(-1). Results detailing the optimisation of the analytical signal, calibration, and common interferences of animal feeds were also discussed. The proposed Fl-CL method was successfully applied to the determination of nitrofurans in animal feeds, with excellent recoveries, as the determination is free from interference. The method validation has been compared versus HPLC method for animal feed samples. (C) 2010 Elsevier Ltd. All rights reserved. Key Words: TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; CHLORAMPHENICOL RESIDUES; BIOLOGICAL-FLUIDS; LC-MS/MS; HONEY; METABOLITES; FURAZOLIDONE; UV FOOD CHEMISTRY Volume: 123 Issue: 3 Pages: 834-839 DOI: 10.1016/j.foodchem. 2010.05.018 Published: DEC 1 2010. 243 Dielectric and Ferroelectric Properties of Pb(Zr(1/2)Ti(1/2))O(3)-Pb(Ni(1/3)Nb(2/3))O(3) Ceramics Under Perpendicular Compressive Stress Unruan Muangjai1, Prasatkhetragarn Anurak2, Ngamjarurojana Athipong1, Laosiritaworn Yongyut1, Ananta Supon1, Guo Ruyan3, Bhalla Amar3 and Yimnirun Rattikorn4 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Materials Science, School of Science & Technology, Naresuan University, Phayao, Thailand. 3 Department of Electrical & Computer Engineering, College of Engineering, University Texas San Antonio, San Antonio, TX 78249 USA. 4 School of Physics, Institute of Science, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract The dielectric and ferroelectric properties of (1-x)Pb(Zr(1/2)Ti(1/2))O(3)-(x) Pb(Ni(1/3)Nb(2/3))O(3) or (1-x)PZT-(x)PNN(x = 0.1-0.5) ceramics were measured under compressive stress applied perpendicular to an electric field direction. For the dielectric properties, the experimental results revealed that the superimposed perpendicular compression stress reduced the dielectric constant and the dielectric loss tangent of all compositions. The stress-induced changes the dissipation energy, the polarizations, however, depended significantly on ceramic compositions, but increases the coercive field of all the compositions. These results were interpreted through the non-180 degrees ferroelastic domain switching processes, the stress clamping of domain walls, de-aging mechanism, and the stress induced enhance in switchable part of spontaneous polarization. Key Words: ZIRCONATE-TITANATE CERAMICS; UNIAXIAL-STRESS; PIEZOELECTRIC PROPERTIES; SINGLE-CRYSTALS; DEPENDENCE INTEGRATED FERROELECTRICS Volume: 114 Pages: 25-34 DOI: 10.1080/10584587. 2010.496600 Published: 2010. 244 Dielectric properties of lead-free solid solution of Bi0.487Na0.487La0.017TiO3 and BaTiO3 Pisitpipathsin Nuttapon, Pengpat Kamonpan, Kantha Puripat, Leenakul Wilaiwan, Eitsayeam Sukum, Rujijanakul Gobwute and Tunkasiri Tawee Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Bismuth sodium titanate (Bi0.5Na0.5TiO3; BNT) is one of the most preferred materials for the preparation of lead-free ceramics; however its dielectric property is lower than that of lead-based materials. Thus, the substitution of A and B-site cations is considered to be one of the best solutions in order to improve both the dielectric and piezoelectric properties. In this study, lead-free ceramics from bismuth sodium lanthanum titanate (Bi0.487Na0.487La0.017TiO3; BNLT) and barium titanate (BaTiO3; BT) systems were prepared by a modified two-step mixed oxide method. BT powder was added to BNLT powder with the desired compositions of (1 - x) BNLT-xBT, where x = 0.00, 0.02, 0.04, 0.06, 0.08, and 0.10. It was found that the addition of BT in the BNLT improved the electrical properties of ceramics. Phase transition of rhombohedral to tetragonal structure was significantly dependent on the amount of BT added. It was also found that the change in crystal structure affected the ferroelectric property of the ceramics where the transition from ferroelectric to antiferroelectric started from the samples with x epsilon 0.06 mol% and higher. This may be useful in terms of using these materials in the desired applications. Key Words: FREE PIEZOELECTRIC CERAMICS; MORPHOTROPIC PHASEBOUNDARY; FERROELECTRIC PROPERTIES; ELECTRICAL-PROPERTIES; SYSTEM PHASE TRANSITIONS Volume: 83 Issue: 10-11 Special Issue: SI Pages: 875-883. Article Number: PII 929858389 DOI:10.1080/01411594.2010.509165 Published: 2010. 245 Dispersion of Particulate Matter (PM10) from Forest Fires in Chiang Mai Province, Thailand Amnauylawjarurn Teerachai1, Kreasuwun Jiemjai2, Towta Sripen1 and Siriwitayakorn Kingkeo1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Graduate Program in Physics & Environmental Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract This research investigates the factors affecting dispersion of particulate matter (PM10) released from forest fires in Chiang Mai province during March 9-13, 2007 and 2008. Atmospheric initial and boundary conditions for this area were generated by the mesoscale model, MM5. Dispersion of the PM10 at the resolution of 1 square kilometer was performed by the air pollution model, CALPUFF. Atmospheric stability over Chiang Mai is clearly indicated by the temperature inversion mid wind velocity shown on Skew-T diagrams derived from the MM5. Dispersion of the PM10 over Chiang Mai vicinity was found to depend on atmospheric stability, wind direction and velocity and its topography. The simulated PM10 concentrations in Chiang Mai were 161-401 mu g/m(3) during March 9-13, 2007 and 32-80 mu g/m(3) during March 9-13, 2008 consistent with the observed values. The PM10 affected areas in Chiang Mai were defined according to the concentrations of the air pollutant. CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 1 Pages: 39-47. Published: JAN 2010. 246 Diversity of Arbuscular Mycorrhizal Fungi in Mimosa invisa and Effect of the Soil pH on the Symbiosis Kittiworawat Saengdao1, Youpensuk Somchit1 and Rerkasem Benjavan2 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Agronomy, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract Mimosa invisa is widely distributed in Northern Thailand. M. invisa is a leguminous weed which is used as green manure for increasing soil fertility and organic matter. They are colonized by arbuscular mycorrhizal (AM) fungi which increase nutrient uptake by the plant. In addition, roots of M. invisa are nodulated with Rhizobium bacteria that fix N(2) from the atmosphere. The objectives of this research are (1) to study diversity of AM fungi in M. invisa in cultivated, uncultivated and seasonally wet areas in Chiang Mai province (2) to evaluate the effect of soil pH (4.0, 5.0 and 6.0) on AM spore production in two varieties of M. invisa (thornless and thorny mimosa) and (3) the effect of AM fungi on the host plant in a pot experiment. Twenty-three species of AM fungi were found in the root zones of M. invisa in the study areas. The highest spore density occurred in uncultivated areas, slightly lower in cultivated areas and lowest in seasonally wet areas. In the pot experiment, AM fungi significantly increased dry weight, nutrient contents (N, P and K) in shoot, root and N in nodule in all treatments. The soil pH had effect on root colonization and spore density. The optimal soil pH for root colonization and spore production of AM fungi was pH 5.0 associated with both varieties of M. invisa. Root colonization in thornless mimosa and thorny mimosa in the soil pH 5.0 was 88% and 80%, respectively. The highest spore density in soil pH 5.0 of thornless mimosa was 16.3 spores g(-1) soil while thorny mimosa was 7.3 spores g(-1) soil. Thornless mimosa had root colonization and spore density of AM fungi higher than thorny mimosa. Therefore, in addition to the ease in handling without the sharp little thorns, the thornless mimosa is more suitable for using as green manure and as natural inoculum of AM fungi in agriculture than thorny mimosa. Key words: arbuscular mycorrhizal fungi; Mimosa invisa; soil pH; Rhizobium CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 3 Pages: 517-527. Published: SEP 2010. 247 Doped Soap Membranes Selectively Permeate a Chiral Isomer Kanyanee Tinakorn1,2,3, Jakmunee Jaroon2,3, Grudpan Kate2,3 and Dasgupta Purnendu K.1 1 Department of Chemistry & Biochemistry, University of Texas Arlington, Arlington, TX 76019 USA. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Center of Excellence for Innovation Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Soap films can not only be made with sub-mu m thickness, they can have intrinsic permeabilities comparable to many commercial membranes, permitting very high transmembrane flux. Soap films readily incorporate a wide range of modifiers to allow highly tailored selectivity: We show that incorporating alpha-cyclodextrin in an 890 nm thick Triton-X-100 film allows, in a single stage, a high degree of chirally selective transport (1.6:1) of alpha(+)-pinene over alpha(-)-pinene. Key Words: ALPHA-CYCLODEXTRIN; LIQUID-CHROMATOGRAPHY; ANALYTICALCHEMISTRY; TRITON X-100; PERVAPORATION; SEPARATION; PHASE; (+)-ALPHAPINENE; THERMODYNAMICS; COMPLEXATION Journal of the American Chemical Society, 2010, 132(51), pp.18045-18047. Published: DEC 2010. 248 Ecological status of the lowland deciduous forest in Chang Kian Valley, Chiang Mai, northern Thailand Vaidhayakarn Chawapich and Maxwell James F. Department of Biology, Faculty of Science, CMU Herbarium, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract An ecological assessment of lowland deciduous dipterocarp-oak, seasonal, hardwood forest on the base of the east side of Doi Sutep-Pui, Chiang Mai province, was conducted. Seven selected sites representing the most intact condition to the worst one were surveyed. Plant species diversity and abundance declined as fire damage increased. Details on the number of species, their habits, size classes of trees, pioneer vs climax species, woody seedlings, coppices, and herbaceous ground flora are presented. Species lists for all these categories and their abundance are also included. Profile diagrams and photographs of some sites are shown. The conservation value of lowland forests has been largely neglected and now many places require reforestation. The degree of degradation is serious and will continue to deteriorate unless effective protective and remedial action is done. Key Words: TOPOGRAPHIC POSITION; SURVIVAL MAEJO INTERNATIONAL JOURNAL OF SCIENCE AND TECHNOLOGY. Volume: 4 Issue: 2 Pages: 268-317 Published: MAY-AUG 2010. 249 Effect of compressive stress on the ferroelectric hysteresis behavior in 0-3 PZT-cement composites Chaipanich Arnon1, Jaitanong Nittaya1 and Yimnirun Rattikorn2 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract Lead zirconate titanate (PZT)-cement composites of 0-3 connectivity were produced using 50% PZT vol. content. PZT-cement based composites are developed for use as sensors for smart concrete structures, however, these sensors would in real life be under a certain stress. The effect of stress on the ferroelectric polarization-electric field (P-E) hysteresis of the composites is reported in this present work. It was found that there was a small but noticeable reduction in the remnant polarization (P(ir)) by approximate to 0.3-0.7 mu C/cm(2) while the coercive field (E(ic)) remained approximately the same when the stress was increased from 0 to 57 MPa. (C) 2009 Elsevier B.V. All rights reserved. Key Words: PIEZOELECTRIC PROPERTIES; PARTICLE-SIZE; MICROSTRUCTURE; POLARIZATION MATERIALS LETTERS Volume: 64 Issue: 5 Pages: 562-564 DOI: 10.1016/j.matlet.2009.11.050 Published: MAR 15 2010. 250 Effect of electrolyzed oxidizing water and continuous ozone exposure on the control of Penicillium digitatum on tangerine cv. ‘Sai Nam Pung’ during storage Whangchai K.1, Saengnil K.1, Singkamanee C.2 and Uthaibutra J.1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract The effect of electrolyzed oxidizing (EO) water in combination with ozone to control postharvest decay of tangerine cv. “Sai Num Pung” was investigated. The spore suspension containing 10(5) conidia ml(-1) of Penicillium digitatum was prepared. EO water was generated by electrolysis of various concentrations of NaCl solution (5, 25, 50% and saturated NaCl). The spore suspension was inoculated into EO water and incubated at 27 degrees C for 1, 2, 4, 8 and 32 min. It was found that the EO water with saturated NaCl completely inhibited the spore germination of the fungus within 1 min. When the fruits inoculated with P. digitatum were washed in EO water at the same concentrations as previous experiment for 4, 8 and 16 min and stored at 5 degrees C for 18 days, it was found that immersion of the fruit in EO water for 8 min was the most effective to reduce disease incidences. Moreover, washing fruit in EO water and kept in a refrigerated chamber at 5 degrees C with continuous ozone exposure at a concentration of 200 mg l(-1) for 2 h day(-1) to extend storage life suppressed the disease incidence until 28 days. However, none of the treatments had any effect on the quality of fruit such as total soluble solids, titratable acidity, percent weight loss and peel color. Therefore EO water may be useful for surface sanitation and ozone has potential to control the recontamination of postharvest diseases in tangerine fruit in storage room. (C) 2009 Elsevier Ltd. All rights reserved. Key Words: POSTHARVEST DECAY; TABLE GRAPES; FRUIT; PATHOGENS; CHLORINE; ROT CROP PROTECTION Volume: 29 Issue: 4 Pages: 386-389 DOI: 10.1016/j.cropro.2009.12.024 Published: APR 2010. 251 Effect of feeding Spirulina platensis on growth and carcass composition of hybrid red tilapia (Oreochromis mossambicus x O. niloticus) Ungsethaphand Thepparath1,3, Peerapornpisal Yuwadee2, Whangchai Niwoot3 and Sardsud Uraporn2 1 Biotechnology Program, Graduated School, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Faculty of Fisheries Technology & Aquatic Resources, Maejo University, Chiang Mai 50290, Thailand. 2 Abstract The present study was designed to evaluate the use of spirulina (Spirulina platensis) as a protein source for hybrid red tilapia. Spirulina was incorporated into four isonitrogenous and isocaloric diets at 0, 5, 10 and 20% of the fishmeal-based diet. The test diets were fed to the fish at 2% body weight twice a day for 120 days. The final weight gain, specific growth rate, feed conversion ratio and survival rate of fish were not affected by spirulina supplementation (p>0.05). There was no significant difference (p>0.05) in carcass proximate composition of the fish fed on spirulina diets as compared to those on control diet. This study demonstrates that up to 20% of spirulina can be substituted for fishmeal in a fishmeal-based diet for hybrid red tilapia without any adverse effect on fish growth. Key Words: RAW SPIRULINA; QUALITY MAEJO INTERNATIONAL JOURNAL OF SCIENCE AND TECHNOLOGY. Volume: 4 Issue: 2 Pages: 331-336 Published: MAY-AUG 2010. 252 Effect of Particle Size on Dielectric and Ferroelectric Properties of 0-3 Lead Magnesium Niobate Titanate-Portland Cement Composites Potong R.1, Rianyoi R.1, Jareansuk L.1, Jaitanong N.1, Yimnirun R.2 and Chaipanich A.1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract The effect of lead magnesium niobate titanate (PMNT) particle size on the dielectric and ferroelectric properties of PMNT- Portland cement (PC) composites was investigated. PMNT of various particle sizes (75-425m) were used at 50% by volume to produce the composites using the mixing and pressing method. Dielectric properties at various frequencies (0.1-20 kHz) and ferroelectrics behavior of the PMNT-Portland cement composites were investigated at room temperature. The results show that the dielectric constant of PMNT- Portland cement composite are found to increase ([image omitted] = 154 for particle size 75 m and [image omitted] = 275 for particle size 425 m (at 1 kHz)), while dielectric loss decreased with increasing particle size of ferroelectric PMNT ceramics. Furthermore, the oinstantaneouso remnant polarization (Pir) of composites was found to increase with increasing PMNT particle size. Key Words: PIEZOELECTRIC PROPERTIES; CERAMICS; TIME FERROELECTRICS Volume: 405 Pages: 98-104 Article Number: PII 930405944 DOI: 10.1080/00150193.2010.482899 Published: 2010. 253 Effect of plasma treatment on hydrophobicity and barrier property of polylactic acid Chaiwong C.1,2, Rachtanapun P.3, Wongchaiya P.3, Auras R.4 and Boonyawan D.1,2 1 Department of Physics & Materials Science, Plasma & Beam Physics Research Facility, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ThEP Center, Bangkok 14000, Thailand. 3 Department of Packaging Technology, Faculty of Ago-Industry, Chiang Mai University, Chiang Mai 50200, Thailand. 4 School of Packaging, Michigan State University, E Lansing, MI 48824 USA. Abstract In this work, we investigated the influence of SF(6) plasma generated by an inductively coupled discharge on the hydrophobicity and barrier properties of poly(lactic acid), PIA. It was found that SF(6) plasma enhanced the hydrophobicity of PLA as observed in the increase of the water contact angle. Water absorption time of the plasma-treated PLA was two-fold increased. However, the plasma treatment did not have significant influence on water vapor permeability of PEA. The differential scanning calorimetry results revealed that the bulk structure which controls transportation property of PLA remained unchanged after plasma treatment. Atomic force microscopy showed topographical modification after plasma treatment. Attenuated total reflectance Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy showed the bounding of fluorines in the PEA structure, which leads to the increase in hydrophobicity of PLA. (C) 2010 Elsevier B.V. All rights reserved. Key words: Plasma treatment; Polylactic acid; Hydrophobicity; Barrier property SURFACE & COATINGS TECHNOLOGY Volume: 204 Issue: 18-19 Pages: 2933-2939 DOI: 10.1016/j.surfcoat.2010.02.048 Published: JUN 25 2010. 254 Effect of Si(3)N(4) nanoparticulates on the mechanical and electrical properties of PZT ceramics Namsar Orapim, Watcharapasorn Anucha and Jiansirisomboon Sukanda Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract PZT/xSi(3)N(4) ceramics (when x = 0, 0.1, 0.5 and 1 wt.%) were prepared by a solid-state mixed-oxide method and sintered at 1125 degrees C for 2 h. X-ray diffraction results suggested that the addition of Si(3)N(4) nanoparticulates did not significantly affect the unit cell and tetragonality of PZT. The addition of 0.1 wt.% Si(3)N(4) effectively increased the density and reduced the grain size of PZT ceramics. These changes played an important role in hardness and fracture toughness improvement. The maximum room temperature dielectric constant was achieved in a PZT/0.1 wt.% Si(3)N(4) sample. Within the Si(3)N(4)-containing samples, the high-temperature dielectric values and ferroelectric properties seemed to increase with increasing concentrations of Si(3)N(4). Key Words: ZIRCONATE-TITANATE CERAMICS; FERROELECTRIC PROPERTIES; COMPOSITES; NANOCOMPOSITES PHYSICA SCRIPTA Volume: T139 Article Number: 014001. DOI: 10.1088/0031-8949/2010/ T139/014001 Published: MAY 2010. 255 Effect of solvents on the microstructure of CaWO(4) prepared by a solvothermal synthesis Kaowphong Sulawan1, Thongtem Titipun2 and Thongtem Somchai3 1 Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Different sizes of nanostructured CaWO(4) have been prepared by a simple solvothermal synthesis at a low temperature of 160 degrees C for 6 h through the reaction between Ca(NO(3))(2)center dot 4H(2)O and Na(2)WO(4)center dot 2H(2) O using various solvents as the reaction medium. The products, characterized by X-ray diffraction (XRD), were specified as pure CaWO(4) with the scheelite structure. Scanning electron microscope (SEM) and transmission electron microscope (TEM) analysis showed that the particle sizes are mainly related to the physical and chemical properties of the solvents used in the solvothermal synthesis. Photoluminescence properties were also investigated. Key words: Nanocrystalline materials; Solvothermal synthesis; CaWO(4) JOURNAL OF CERAMIC PROCESSING RESEARCH Volume: 11. Issue: 4 Pages: 432-436 Published: AUG 2010. 256 Effect of Temperature on the Dielectric Properties of 0-3 PZT-Cement Composites Chaipanich A., Rianyoi R., Potong R. and Jaitanong N. Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Abstract In this work, 0-3 lead zirconate titanate (PZT) was mixed with normal Portland cement to produce 0-3 connectivity composites. The effect of temperature on the dielectric properties such as the dielectric constant and dielectric loss was determined. It was found that with increasing PZT content the T(c) increases where the optimum dielectric constant was observed and that at the temperature up to 100 degrees C there is a significant change in the dielectric properties in PZT-cement composites. This is due to the loss of water molecules at up to 100 degrees C. At above 100 degrees C, the dielectric properties of the composites were found to have a similar behavior to that of PZT ceramic with Tc being approximate to 420 degrees C. Key Words: PIEZOELECTRIC PROPERTIES; PARTICLE-SIZE FERROELECTRICS LETTERS SECTION Volume: 37 Issue: 4 Pages: 76-81. DOI: 10.1080/07315171.2010.527799 Published: 2010. 257 Effects of Beryllium on Microstructure and Resiliency of Silver-Copper Alloy Chairuangsri Torranin2 and Nisaratanaporn Ekasit1 1 Department of Metallurgical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand. 2 Department of Industrial Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Effects of beryllium on microstructure and resiliency of cast Ag-Cu alloy containing 93.5 wt% Ag were investigated. Beryllium has an effect on modification of the eutectic structure from the typical lamellar structure to be apparently discrete Ag-rich phase encapsulated in the Cu-rich constituent. Transmission electron microscopy revealed that precipitation of fine (>20nm) fcc precipitates were observed in the dendritic areas of the Be-containing alloy after ageing heat treatment. Modulus of resilience was used to determine resiliency of the alloys. The modulus of resilience of the Be-containing alloy was about two times that of the alloy without Be addition. This is believed to relate to the precipitation of fine fcc precipitates in the dendritic areas and the modification of eutectic structure in the Be-containing alloy. CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 2 Pages: 260-268 Published: MAY 2010. 258 Effects of bromelain on cellular characteristics and expression of selected genes in canine in vitro chondrocyte culture Siengdee P.2, Nganvongpanit K.1, Pothacharoen P.3, Chomdej S.2, Mekchay S.4 and Ong-Chai S.3 1 Department of Veterinary Biosciences & Public Health, Bone & Joint Research Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand. 2 Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The purpose of this study was to determine the effect of bromelain treatment on canine articular chondrocytes in vitro. This research evaluated cell viability, levels of apoptotis and mitotis, proteoglycan concentrations and the expression of certain genes. Chondrocytes were exposed to 50 mu g/ml bromelain for 4, 16 and 32 h. The rate of apoptotis in the treatment groups was significantly lower than in the control groups that were incubated with media only (P < 0.05); and the mitotic rate in treatment groups was significantly higher than in the control groups (P < 0.05), at all durations of exposure. The effect of bromelain on gene expression was measured by the real-time PCR technique. It was found that bromelain significantly decreased (P < 0.05) TIMP-1 and MMP-3 expression. These experimental bromelain treatments have shown positive results, and have increased the basic knowledge in regard to the healing and modulation of osteoarthritis, prior to the general use of bromelain in clinical practice. Key Words: COMBINATION THERAPY; SURFACE MOLECULES; CYCLOSPORINE-A; T-CELLS; ACTIVATION; OSTEOARTHRITIS; ARTHRITIS; MODULATION; CARTILAGE; DISEASE VETERINARNI MEDICINA Volume: 55 Issue: 11 Pages: 551-560 Published: 2010. 259 Effects of Mn and Cu Doping on Electrical Properties of 0.75BiFeO(3)-0.25BaTiO(3) Ceramics Chandarak S.2, Ngamjarurojana A.3, Pojprapai S.2, Srilomsak S.2, Rujirawat S.1,4 and Yimnirun R.1 1 Institute of Science, School Physics, Suranaree University Technology, Nakhon 30000, Ratchasima, Thailand. 2 Institute of Engineering, School of Ceramic Engineering, Suranaree University Technology, Nakhon 30000, Ratchasima, Thailand. 3 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Public Organization, Synchrotron Light Research Institute, Nakhon 30000, Ratchasima, Thailand. Abstract In this study, 0.75BiFeO(3)-0.25BaTiO(3) ceramics doped with Mn and Cu (0, 1, 2, 3, 4 and 5 mol%) have been fabricated with an effective solid-state reaction method developed for high purity ceramics. Attention has been focused on relationships between phase formation, density, dielectric and ferroelectric properties of the sintered ceramics. It was found that Mn and Cu doping affected the dielectric and ferroelectric properties of these ceramics. In addition, Cu and Mn doping was also found to improve the sintering ability and change the structural symmetry of this system from rhombohedral to tetragonal. Key Words: CRYSTAL; BIFEO3 INTEGRATED FERROELECTRICS Volume: 114 Pages: 100-107 DOI: 10.1080/10584587. 2010.488192 Published: 2010. 260 Effects of Parallel and Perpendicular Compressive Stresses on the Dielectric and Ferroelectric Properties of Soft PZT Ceramics Unruan Muangjai1, Ananta Supon1, Laosiritaworn Yongyut1, Ngamjarurojana Athipong1, Guo Ruyan2, Bhalla Amar2 and Yimnirun Rattikorn3 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Electrical & Computer of Engineering, College of Engineering, University of Texas San Antonio, San Antonio, TX 78249 USA. 3 Institute of Physics, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract Effects of compressive stress on the dielectric and ferroelectric properties of commercial soft PZT ceramics were investigated. The dielectric and ferroelectric properties were measured under compressive stress applied parallel and perpendicular to an electric field direction. The results showed that the dielectric properties; i.e. the dielectric constant (epsilon(r)) and dielectric loss tangent (tan delta), and the ferroelectric characteristics; i.e. the area of ferroelectric hysteresis (P-E) loops, the maximum polarization (P(max)), the coercive field (E(c)) and the remanent polarization (P(r)), changed significantly with increasing compressive stress. These changes depended greatly on direction of the applied stress. The stress clamping of domain wall, de-ageing and non-180 degrees ferroelectric domain switching processes are responsible for the changes observed. In addition, a significant decrease in those parameters after a full cycle of stress application has been observed and attributed to the de-poling and stress-induced decrease in switchable part of spontaneous polarization at high stress. This study clearly showed that the applied stress has significant influence on the electrical properties of soft PZT ceramics. Key Words: ZIRCONATE-TITANATE CERAMICS; UNIAXIAL-STRESS; ELECTROMECHANICAL PROPERTIES; PIEZOELECTRIC PROPERTIES; DEPENDENCE; BEHAVIOR; CRYSTALS; FIELD FERROELECTRICS Volume: 400 Pages: 144-154 DOI: 10.1080/00150193.2010.505517 Published: 2010. 261 Effects of vibro-milling on relaxor ferroelectric behavior and phase transition of lead-free Ba(Zr0.25Ti0.75)O3 ceramics Kruea-In C.1, Eitssayeam S.1, Pengpat K.1, Rujijanagul G.1,2 and Tunkasiri T.1,3 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Science & Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Abstract Lead-free Ba(Zr0.25Ti0.75)O3 ceramics were synthesized from powders prepared by a conventional mixed oxide and vibro-milling method to investigate the phase transition, dielectric response, and ferroelectric properties of the prepared samples. Compared to a conventional sample, the samples prepared by the vibro-milling method showed a higher dielectric constant at the ferroelectric-toparaelectric transition temperature. The vibro-milling method also produced a stronger frequency dependence on the dielectric constant. To confirm the dielectric properties and phase transition behavior, ferroelectric hysteresis measurements were carried out in the temperature range -40 degrees C to 80 degrees C. The microstructural properties of the samples were investigated and the results were then correlated with the characteristics of the milled and calcined powder as well as the grains of the ceramics. Key Words: TITANATE CERAMICS; GRAIN-SIZE PHASE TRANSITIONS Volume: 83 Issue: 10-11 Special Issue: SI Pages: 942-949. Article Number: PII 929856420 DOI: 10.1080/01411594.2010.509604. Published: 2010. 262 Effects of vibro-milling time on phase formation and particle size of Zn(3)Nb(2)O(8) nanopowders Prasatkhetragarn A.1, Ketsuwan P.1, Ananta S.2 and Yimnirun R.3 1 School of Science & Technology, Naresuan Phayao University, Phayao 56000, Thailand. Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. 2 Abstract A monoclinic phase of zinc niobate, Zn(3)Nb(2)O(8), nanopowder was synthesized by a solid-state reaction via a rapid vibro-milling technique. The effect of milling time on the phase formation and particle size of Zn(3)Nb(2)O(8) powder was investigated. The formation of the Zn(3)Nb(2)O(8) phase in the calcined powders has been investigated using DTA and XRD techniques. Morphology, particle size and chemical composition have been determined via a combination of SEM and laser diffraction techniques. It was found that an average particle size was achieved at 20 h of vibro-milling after which a higher degree of particle agglomeration was observed on continuation of milling to 30 h. In addition, by employing an appropriate choice of the milling time, a narrow particle size distribution curve was also observed. (C) 2010 Elsevier B.V. All rights reserved. Key Words: MICROWAVE DIELECTRIC-PROPERTIES; SOLID-STATE REACTION; CALCINATION CONDITIONS; POWDERS; CERAMICS MATERIALS LETTERS Volume: 64 Issue: 9 Pages: 1113-1116. DOI: 10.1016/j.matlet.2010.01.088 Published: MAY 15 2010. 263 Effects of ZnO Nanowhiskers Addition on Microstructure and Dielectric Properties of Lead Titanate Ceramics Wongmaneerung R.1, Choopan S.2, Yimnirun R.3 and Ananta S.2 1 Department of Physics, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand. Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 School of Physics, Institute of Science, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. 2 Abstract Ceramic nanocomposites of zinc oxide (ZnO) nanowhiskers in lead titanate (PbTiO3 or PT) matrix were successfully fabricated by a pressureless sintering technique. Phase formation, densification, microstructure and dielectric properties of the composites were investigated as a function of ZnO content. It has been found that a densification of the ceramic nanocomposites fabricated in this work was significantly enhanced, as compared to the conventional PT ceramics. Moreover, the high dielectric constant, which is higher than that of the monolithic PT ceramics, was found in the PT/ZnO ceramic nanocomposites. Key Words: COMPOSITES; FABRICATION FERROELECTRICS Volume: 403 Pages: 150-157 Article Number: PII 930389663. DOI: 10.1080/00150191003750853 Published: 2010. 264 Efficacy and Safety of 1-Month Postpartum ZidovudineDidanosine to Prevent HIV-Resistance Mutations after Intrapartum Single-Dose Nevirapine Lallemant Marc1,21,3, Ngo-Giang-Huong Nicole1,21,3, Jourdain Gonzague1,21,3, Traisaithit Patrinee1,3,4, Cressey Tim R.1,21,3, Collins Intira J.1,21,3, Jarupanich Tapnarong6, Sukhumanant Thammanoon7, Achalapong Jullapong8, Sabsanong Prapan9, Chotivanich Nantasak10, Winiyakul Narong, Ariyadej Surabon13, Kanjanasing Annop14, Ratanakosol Janyaporn12, Hemvuttiphan Jittapol15, Kengsakul Karun16, Wannapira Wiroj19, Sittipiyasakul Veerachai5, Pornkitprasarn Witaya11, Liampongsabuddhi Prateung20, McIntosh Kenneth22, Van Dyke Russell B.23, Frenkel Lisa M.24,25, Koetsawang Suporn17, Le Coeur Sophie2, Kanchana Siripon18 and PHPT-4 Study Team 1 University of Paris 05, Institute of Research for Development, U174, Institut National d’Études Démographiques, F-75006 Paris, France. 2 University of Paris 05, Unite Mixte Research 196, Institute of Research for Development, Institut National d’ Études Démographiques, Center Français de la Population et du Developpement, F-75006 Paris, France. 3 Chiang Mai University, Faculty of Associated Medical Sciences, Chiang Mai 50200, Thailand. 4 Chiang Mai University, Department of Statistics, Faculty of Science, Chiang Mai 50200, Thailand. 5 Health Promotion Center Region 10, Chiang Mai, Thailand. 6 Hat Yai Hospital, Hat Yai, Songkla, Thailand. 7 Samutsakhon Hospital, Samutsakhon, Thailand. 8 Chiangrai Prachanukroh Hospital, Chiangrai, Thailand. 9 Samutprakarn Hospital, Samut Prakan, Thailand. 10 Chonburi Hospital, Chon Buri, Thailand. 11 Somdej Pranangchao Sirikit Hospital, Chon Buri, Thailand. 12 Khon Kaen Hospital, Khon Kaen, Thailand. 13 Rayong Hospital, Rayong, Thailand. 14 Chacheongsao Hospital, Chacheongsao, Thailand. 15 Phayao Provincial Hospital, Phayao, Thailand. 16 Bhumibol Adulyadej Hospital, Bangkok, Thailand. 17 Mahidol University, Family Health Research Center, Bangkok 10700, Thailand. 18 Ministry of Public Health, Bangkok, Thailand. 19 Buddhachinaraj Hospital, Phitsanulok, Thailand. 20 Lampang Hospital, Lampang, Thailand. 21 Harvard University, School of Public Health, Department of Immunology and Infectious Diseases, Boston, MA 02115 USA. 22 Harvard University, School of Medicine, Department of Pediatrics, Childrens Hospital Boston, Boston, MA 02115 USA. 23 Tulane University, Health Sciences Center, Department of Pediatrics, New Orleans, LA 70118 USA. 24 University of Washington, Childrens Hospital, Seattle, WA 98195 USA. 25 University of Washington, Regional Medical Center, Séattle, WA 98195 USA. 265 Abstract Background: Intrapartum single-dose nevirapine plus third trimester maternal and infant zidovudine are essential components of programs to prevent motherto-child transmission of human immunodeficiency virus (HIV) in resource-limited settings. The persistence of nevirapine in the plasma for 3 weeks postpartum risks selection of resistance mutations to nonnucleoside reverse-transcriptase inhibitors (NNRTIs). We hypothesized that a 1-month zidovudine-didanosine course initiated at the same time as single-dose nevirapine (sdNVP) would prevent the selection of nevirapine-resistance mutations. Methods: HIV-infected pregnant women in the PHPT-4 cohort with CD4 cell counts >250 cells/mm(3) received antepartum zidovudine from the third trimester until delivery, sdNVP during labor, and a 1-month zidovudine-didanosine course after delivery. These women were matched on the basis of baseline HIV load, CD4 cell count, and duration of antepartum zidovudine to women who received sdNVP in the PHPT-2 trial (control subjects). Consensus sequencing and the more sensitive oligonucleotide ligation assay were performed on samples obtained on postpartum days 7-10, 37-45, and 120 (if the HIV load was >500 copies/mL) to detect K103N/Y181C/G190A mutations. Results: The 222 PHPT4 subjects did not differ from matched control subjects in baseline characteristics except for age. The combined group median CD4 cell count was 421 cells/mm(3) (interquartile range [IQR], 322-549 cells/mm3), the median HIV load was 3.45 log(10) copies/mL (IQR, 2.79-4.00 log(10) copies/mL), and the median duration of zidovudine prophylaxis was 10.4 weeks (IQR, 9.1-11.4 weeks). Using consensus sequencing, major NNRTI resistance mutations were detected after delivery in 0% of PHPT-4 subjects and 10.4% of PHPT-2 controls. The oligonucleotide ligation assay detected resistance in 1.8% of PHPT-4 subjects and 18.9% of controls. Major NNRTI resistance mutations were detected by either method in 1.8% of PHPT-4 subjects and 20.7% of controls (P<.001). Conclusions: A 1-month postpartum course of zidovudine plus didanosine prevented the selection of the vast majority of NNRTI resistance mutations. Key Words: TO-CHILD TRANSMISSION; ACTIVE ANTIRETROVIRAL THERAPY; HEPATITIS-B; VERTICAL TRANSMISSION; RECEIVING NEVIRAPINE; RANDOMIZED-TRIAL; SUBTYPE-A; WOMEN; EXPOSURE; DISCONTINUATION CLINICAL INFECTIOUS DISEASES Volume: 50 Issue: 6 Pages: 898-908. DOI: 10.1086/ 650745 Published: MAR 15 2010. 266 Electrical and Aging Properties of Doped Barium Titanate Ceramics Dechakupt T.1,2, Tangsritrakul J.3, Unruan M.1, Ketsuwan P.4, Srisombat L.5, Ananta S.1 and Yimnirun R.6 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 3 Department of Physics, Faculty of Science & Technology, Thammasat University, Prathumtani, Thailand. 4 Department of Physics, School of Science & Technology, Naresuan University, Phayao, Thailand. 5 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 6 School of Physics, Institute of Science, Suranaree University Technology, Nakhon Ratchasima, Thailand. Abstract The tetragonal structured Ba(Ti1-xFex)O3 ceramics where x = 0, 0.005 and 0.01 were prepared by conventional mix-oxide method. Aging of dielectric properties and ferroelectric hysteresis loop was studied as a function of time. It was found that two aging stages might be existed in the aging process. The effect of Fe doping in barium titanate is not clearly seen in the aging of the dielectric properties while the effect obviously has influence on the P-E hysteresis loops. It is shown that the P-E loop of Fe doped compositions become constricted after aging. The remanent polarization and coercive field decreases as a function of time. The decreasing rate is higher for higher content of Fe dopant. Key Words: BaTiO3; FERROELECTRICS; BEHAVIOR FERROELECTRICS Volume: 403 Pages: 97-103 Article Number: PII 930401359. DOI: 10.1080/00150191003748980. Published: 2010. 267 ELECTRICAL AND MECHANICAL PROPERTIES OF PZT/PVDF 0-3 COMPOSITES Thongsanitgarn Pailyn, Watcharapasorn Anucha and Jiansirisomboon Sukanda Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Granules of in-house prepared PZT ceramic and powder of commercially available PVDF polymer were used as starting materials to form a series of xPZT/ (1 - x) PVDF composites (where x = 0, 0.1, 0.3, 0.5, 0.7, 0.9, and 1.0) with 0-3 connectivity. Densities of the composites tended to increase with increasing PZT ceramic content. Phase and microstructure of the composites revealed homogeneous mixture between PZT and PVDF phases. The composites with higher ceramic content had higher dielectric constant and dielectric loss tangent. Ferroelectric measurement revealed the effect of PZT phase connectivity in 0.9PZT/0.1PVDF ceramic in which a sudden jump in ferroelectric properties was observed. Mechanical properties in terms of hardness, Young’s modulus and fracture toughness were also improved when PZT content was increased. Key Words: POLYMER COMPOSITES SURFACE REVIEW AND LETTERS Volume: 17 Issue: 1 Pages: 1-7. DOI: 10.1142/ S0218625X10013540. Published: FEB 2010. 268 Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films Sriwichai Saengrawee1,2,3,4, Baba Akira1,2, Phanichphant Sukon3,4, Shinbo Kazunari1,2, Kato Keizo1,2 and Kaneko Futao1,2 1 Center for Transdisciplinary Research, Niigata University, Nishi Ku, Niigata 9502181, Japan. Graduate School of Science & Technology, Niigata University, Nishi Ku, Niigata 9502181, Japan. 3 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Faculty of Science Center for Innovation in Chemistry, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract An electrochemically controlled surface plasmon resonance (SPR) immunosensor based on carboxylated polyaniline was constructed for detecting human immunoglobulin G (IgG) without using further labeled molecules. The carboxylated polyaniline, poly(3-aminobenzoic acid) (PABA), was prepared by electropolymerization of its monomer, 3-aminobenzoic acid (ABA), on a gold-coated high-refractive-index glass slide that was also used as a working electrode for the electrochemical system. The kinetic properties during electropolymerization were monitored in situ with electrochemical-surface plasmon resonance (EC-SPR) spectroscopy. The obtained PABA film, which was used to construct the immunosensor, showed electroactivity in neutral phosphate-buffered saline solution. The carboxylic acid surface of the PABA film was then activated to immobilize anti-human IgG. Finally, the immunosensor electrode was used to probe the binding reaction of anti-human/human IgG with various concentrations of human IgG at different constant applied potentials. The immobilization and binding reaction processes were controlled and also studied in situ by EC-SPR spectroscopy. The binding process at different applied potentials was studied and fitted using the Freundlich model. The surface morphology of the films after the immobilization of anti-human IgG was studied by atomic force microscopy. (c) 2010 Elsevier B.V. All rights reserved. Key words: Surface plasmon resonance immunosensor; Electrochemical-surface plasmon resonance (EC-SPR) spectroscopy; Poly(3-aminobenzoic acid) (PABA); Anti-human IgG/human IgG binding; Human IgG; Label-free detection SENSORS AND ACTUATORS B-CHEMICAL Volume: 147 Issue: 1 Pages: 322-329. DOI: 10.1016/j.snb.2010.03.031 Published: MAY 18 2010. 269 Endophytic actinomycetes isolated from Aquilaria crassna Pierre ex Lec and screening of plant growth promoters production Nimnoi Pongrawee1, Pongsilp Neelawan2 and Lumyong Saisamorn1 1 Department of Biology, Division Microbiology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand. Abstract A total of 10 endophytic actinomycete strains were successfully isolated from healthy shoots and roots of Aquilaria crassna Pierre ex Lec (eaglewood). Analysis of 16S rDNA sequencing of those isolates showed that they belong to members of the genera Streptomyces (2 isolates), Nonomuraea (1 isolate), Actinomadura (1 isolate), Pseudonocardia (1 isolate) and Nocardia (3 isolates). The remaining 2 isolates were unidentified. All of isolates produced the amount of indole-3-acetic acid (IAA) and ammonia ranging between 9.85 +/- A 0.31 to 15.14 +/- A 0.22 mu g ml(-1) and 2 to 60 mg ml(-1), respectively. Among 10 isolates tested, the amount of hydroxamate-type siderophore produced by 2 isolates was undetectable. While the remaining 8 isolates produced the amount of hydroxamate-type ranging between 3.21 +/- A 0.12 and 39.30 +/- A 0.40 mu g ml(-1). Also, catechols-type siderophore produced by 9 isolates was undetectable. Actinomadura glauciflava is only one isolate that produced catechols-type 4.12 +/- A 0.90 mu g ml(-1). In addition, 10 endophytic actinomycetes showed protease activity ranging from undetectable to 8.16 +/- A 0.15 unit ml(-1). Genetic relatedness amongst these isolates was determined base on Random amplified polymorphic DNA (RAPD) and Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC PCR). Both methodologies generated specific patterns corresponding to particular genotypes. RAPD fingerprinting proved to be slightly more discriminatory than ERIC PCR. This study is the first published report that actinomycetes can be isolated as endophytes within this plant. It is also the first published report that endophytic actinomycetes are capable of producing IAA and siderophores. Key Words: ROOTS; STREPTOMYCETE; BIOCONTROL; BACTERIA; FUNGI; SOIL; RHIZOSPHERE; DIVERSITY; SEQUENCES; SOFTWARE WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY Volume: 26 Issue: 2 Pages: 193-203. DOI: 10.1007/s11274-009-0159-3. Published: FEB 2010. 270 Enhanced Photocurrent Generation in Nanostructured Chromophore/Carbon Nanotube Hybrid Layer-by-Layer Multilayers Baba Akira1,2, Matsuzawa Taihei1,2, Sriwichai Saengrawee1,2,3, Ohdaira Yasuo1,2, Shinbo Kazunari1,2, Kato Keizo1,2, Phanichphant Sukon3,4 and Kaneko Futao1,2 1 Center for Transdisciplinary Research, Niigata University, Nishi Ku, Niigata 9502181, Japan. Graduate School of Science & Technology, Niigata University, Nishi Ku, Niigata 9502181, Japan. 3 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Center for Innovation Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract In this paper, we demonstrate photocurrent generation from nanostructured layer-by-layer (LbL) ultrathin films consisting of chromophores and single-walled carbon nanotubes (SWNT). We fabricated 5,10,15,20-tetrakis(1-methy1-4-pyridinio) porphyrin tetr (p-toluenesulfonate) (TMPyP)-SWNT/sodium copper chlorophyllin (SCC)-SWNT LbL film from noncovalently adsorbed composites. SWNT were dissolved in water-soluble cationic TMPyP and anionic SCC, and the resulting solutions were used for electrostatic LbL multilayer fabrication. The solubility of SWNT in water was studied by UV-vis absorption spectroscopy. The composites were highly dispersed owing to the pi-pi interactions. The fluorescence spectroscopy measurements showed efficient quenching of TMPyP and SCC fluorescence, which was due to the interaction with SWNT. In situ surface plasmon resonance spectroscopy during the LbL multilayer fabrication indicated a stepwise increase in reflectivity, implying the successive formation of nanostructured hybrid ultrathin films. Cyclic voltammetry revealed that the electroactivity of the hybrid film was enhanced by the incorporation of SWNT. The composite LbL film electrode exhibited an enhancement of photocurrent compared to a TMPyP/SCC (no SWNT) film electrode, suggesting efficient charge separation and electron transfer in the system. Key Words: WALLED CARBON NANOTUBES; SURFACE-PLASMON RESONANCE; PHOTOINDUCED ELECTRON-TRANSFER; QUARTZ-CRYSTAL MICROBALANCE; DONOR-ACCEPTOR NANOHYBRIDS; ULTRATHIN POLYMER-FILMS; POLY(3,4ETHYLENEDIOXYTHIOPHENE) FILMS; ASCORBIC-ACID; SPECTROSCOPY; PORPHYRIN JOURNAL OF PHYSICAL CHEMISTRY C Volume: 114 Issue: 35 Pages: 14716-14721 DOI: 10.1021/jp103121m. Published: SEP 9 2010. 271 Enhanced piezoelectric properties and lowered sintering temperature of Ba(Zr(0.07)Ti(0.93))O(3) by B(2)O(3) addition Jarupoom P., Pengpat K. and Rujijanagul G. Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The effects of B(2)O(3) doping on densification, ferroelectric, and piezoelectric properties of Ba(Zr(0.07)Ti(0.93))O(3) (BZT) ceramics were investigated. The addition of B(2)O(3) to the ceramics lowered the sintering temperature by similar to 200 degrees C as well as changed their microstructures. Higher B(2) O(3) concentration caused a decrease in remanent polarization and coercive field, while the piezoelectric coefficient d(33) remained at a high value of 291 pC/N for the ceramic sample with 2 wt.% B(2)O(3). The relationship between piezoelectric properties and ferroelectric constant was examined. (C) 2009 Elsevier B.V. All rights reserved. Key Words: DIELECTRIC-PROPERTIES; FERROELECTRIC PROPERTIES; BA0.7SR0.3TIO3 CERAMICS; TITANATE CERAMICS; BARIUM-TITANATE; BEHAVIOR; SYSTEM; VAPOR CURRENT APPLIED PHYSICS Volume: 10 Issue: 2 Pages: 557-560. DOI: 10.1016/j. cap.2009.07.020. Published: MAR 2010. 272 Enhancement of Ethanol Sensing Properties by Alloying TiO(2) With ZnO Tetrapods Santhaveesuk Theerapong1,2, Wongratanaphisan Duangmanee1,2 and Choopun Supab1,2 1 Department of Physics & Materials Science, APRL, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. Abstract The Ti(x)Zn(1-x)O tetrapods were synthesized using simple thermal oxidation method from Zn and TiO(2) mixture. The tetrapods exhibited single crystalline hexagonal wurtzite structure with the prefer growth direction of [0002] along the legs. The sensors based on Ti(x)Zn(1-x)O tetrapods were fabricated and investigated the ethanol sensing properties. The FE-SEM, HRTEM, SAED, XRD, and RS results suggested that Ti(x)Zn(1-x)O alloy was formed with a slightly decrease of c-axis lattice parameter. The decrease of sensor resistance under ethanol atmosphere was observed and suggested that the Ti(x)Zn(1-x)O tetrapods possessed n-type property of semiconductor similar to ZnO. The Ti(x)Zn(1-x)O tetrapod sensors exhibited higher sensitivity than that of pure ZnO tetrapod sensors for entire ethanol concentration with optimum operating temperature of 300 degrees C. Thus, the enhancement of sensitivity due to alloying TiO(2) with the ZnO tetrapods was observed and maybe explained by an increase of adsorbed oxygen ions due to substitution of Ti atom into Zn atom. Also, the slope value of the plot between log(S - 1) and log C suggested that adsorbed oxygen ion species at the surface of the Ti(x)Zn(1-x)O tetrapods was O(2)-which was same as pure ZnO tetrapods. Finally, these results have an important implication for a development of ethanol sensors based on metal oxide semiconductors for alcohol breath analyzer. Key Words: RAMAN-SCATTERING; DOPED ZNO; FILMS; PHOTOLUMINESCENCE; NANOSTRUCTURES; SENSOR IEEE SENSORS JOURNAL Volume: 10 Issue: 1 Pages: 39-43. DOI: 10.1109/JSEN.2009. 2036048. Published: JAN 2010. 273 Enhancement of Ethanol Sensing Properties by Impregnating Platinum on Surface of ZnO Tetrapods Hongsith Niyom1,2 and Choopun Supab1,2 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. Abstract ZnO tetrapods with a cross-sectional size of about 200-1000 nm were synthesized via an oxidation reaction technique. The sensors based on ZnO tetrapods and platinum impregnated ZnO tetrapods were fabricated and investigated for ethanol sensing properties. The gas sensing properties of the sensors were investigated for ethanol concentration of 50-1000 ppm at different operating temperatures. It was found that the sensitivities of platinum impregnated ZnO tetrapod sensors were higher than that of pure ZnO tetrapod sensors. The enhancement of sensitivity due to platinum impregnation to ZnO tetrapods may be explained either by an increase of adsorbed oxygen density or an increase of reaction rate coefficient in a rate equation for an ethanol adsorption reaction on the ZnO surface. Also, the slope value of the plot between log (S-1) and log C suggested that adsorbed oxygen ion species at the surface of the platinum impregnated ZnO tetrapods was which was O(2-) the same as pure ZnO tetrapods. Finally, these results have an important implication for a development of ethanol sensors based on metal oxide semiconductors for alcohol breath analyzers. Key Words: SENSOR; NANORODS; FABRICATION; NANOSTRUCTURES; NANOBELTS; WHISKER; OXIDES IEEE SENSORS JOURNAL Volume: 10 Issue: 1 Pages: 34-38. DOI: 10.1109/JSEN.2009. 2035746. Published: JAN 2010. 274 Enhancement of sensor response by TiO(2) mixing and Au coating on ZnO tetrapod sensor Santhaveesuk Theerapong1,2, Wongratanaphisan Duangmanee1,2 and Choopun Supab1,2 1 Department of Physics & Materials Science, Faculty of Science, Applied Physics Research Laboratory, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. Abstract The sensors based on ZnO tetrapods (T-ZnO) and ZnO tetrapods with TiO(2) mixing (T-TiZnO) and Au coating (Au/T-TiZnO) were investigated. The T-ZnO and T-TiZnO were synthesized by a simple thermal oxidation technique. The leg of the tetrapod is about 1-10 mu m in length and 160-500 nm in diameter. Au coating was performed by sputtering technique and annealed at 550 degrees C for 6 h under normal atmosphere. The ethanol sensor response of the T-ZnO, T-TiZnO and Au/TTiZnO sensors was tested at the operating temperature of 260-360 degrees C with the ethanol concentration of 50, 100, 200, 500, and 1000 ppm. It was found that at the optimum temperature the Au/T-TiZnO sensor exhibited the highest sensor response and the T-TiZnO sensor exhibited higher sensor response than that of the T-ZnO sensor. The Au/T-TiZnO sensor exhibited the highest sensor response up to 240 at 340 degrees C under the ethanol concentration of 1000 ppm. The enhancement of the sensor response based on the T-ZnO by TiO(2) mixing and Au coating can be explained by alloying effect and metal catalytic effect, respectively. The response enhancement can be explained in terms of electron concentration, no in air and reaction rate constant, k(Eth). The evidence for both effects can be found in higher sensor resistance in air and lower sensor resistance in the ethanol ambient. (C) 2010 Elsevier B.V. All rights reserved. Key words: Gas sensor; ZnO; TiO(2); Tetrapod; Sensor response SENSORS AND ACTUATORS B-CHEMICAL Volume: 147 Issue: 2 Pages: 502-507. DOI: 10.1016/j.snb.2010.03.081 Published: JUN 3 2010. 275 Environmental and biological factors affect desiccation tolerance of algae from two rivers (Thailand and New Zealand) with fluctuating flow Bergey Elizabeth A.1, Bunlue Pensri2, Silalom Somyot2, Thapanya Decha2 and Chantaramongkol Porntip2 1 Department of Zoology, University of Oklahoma, Norman, OK 73019 USA. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract Flow variation associated with hydropower production causes periodic exposure of zones along the banks of regulated rivers. These zones have reduced algal biomass and lower productivity. We investigated whether algal assemblages in regulated rivers differed in tolerance to aerial exposure because understanding such tolerance could be used to alter water releases to improve downstream productivity. In field experiments, we tested algal tolerance to aerial exposure in 3 assemblages: green filamentous and cyanobacterial assemblages in Thailand and a mixed (filamentous green + cyanobacteria) assemblage in New Zealand. Algaebearing stones were exposed at night, during the day (in sun or shade, with or without simulated rain), or continuously, or were continuously submerged (with a handling control). Exposure reduced cyanobacterial chlorophyll a, and reduction was greater with day than with night exposure. Mixed algal assemblages showed a similar pattern in chlorophyll a, but ash-free dry mass (AFDM) did not change (bleached algae remained). In contrast, green filamentous algae survived better when exposed than when submerged, and when exposed during the day than at night. Snails consumed green filamentous algae, and submersion resulted in high grazing loss of filamentous algae but not cyanobacteria. Grazing pressure was presumably lower at night during water release. For cyanobacteria, shade and rain slightly increased chlorophyll a retention. For filamentous green algae, shade had no effect, and rain was beneficial in the shade but not the sun. Our experiments demonstrated an interaction among variable hydraulic conditions, activity of grazing snails, and availability of grazing-susceptible algae. A longer wet dry cycle, daytime water release, and a shaded riparian zone reduced aerial exposure effects on algal assemblages, indicating that water releases can be regulated to improve productivity in the downstream exposed zone. Key words: hydropower peaking; varial zone; regulated rivers; stream drying; benthic algae; periphyton ecology; algal grazing JOURNAL OF THE NORTH AMERICAN BENTHOLOGICAL SOCIETY Volume: 29 Issue: 2 Pages: 725-736. DOI: 10.1899/09-068.1 Published: JUN 2010. 276 Estrogen bioassy of Pueraria mirifica Airy Shaw and Suvatabandhu Siangcham Tanapan, Saenphet Supap and Saenphet Kanokporn Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50000, Thailand. Abstract Pueraria mirifica Airy Shaw & Suvatabandhu has been used by Thai women for centuries as a rejuvenating herb. The scientific claim for its phytoestrogenic property leads to the popular use of this plant in several herbal formulations. This research project was carried out to examine estrogenic activity of P. mirifica from Lampang Province, Thailand, one of the areas that it is harvested for using as raw material in herbal industries. Estrogen bioassay of aqueous extract from P. mirifica at concentrations of 0.1 and 0.2 g/ml was performed in immature ovariectomized mice in comparison to both the negative and positive control groups. Vaginal orifice of mice treated with the extract in all groups and with the positive control group which injected with estradiol benzoate (EB) opened earlier than the negative control mice and vaginal cornification was also found in these 3 groups only. Moreover, the extract significantly increased uterine weight and uterine epithelium height as compared to negative control group with the comparable degree of increment to those of EB treated group. The data of this present study indicated that dried powder of P. mirifica which is used as raw material for herbal products of Lampang Province, exhibited the potent estrogenic activity. Key Words: FEMALE JOURNAL OF MEDICINAL PLANTS RESEARCH Volume: 4 Issue: 9. Pages: 741-744. Published: MAY 4 2010. 277 Ethanol-Promoted Fabrication of Tungsten Oxide Nanobelts with Defined Crystal Orientation Daothong Suphaporn2,3, Songmee Naratip2, Dejang Nuchjira2, Pichler Thomas1, Shiozawa Hidetsugu4, Jia Yan5, Batchelor David6, Kauppinen Esko3, Thongtem Somchai2, Ayala Paola1 and Singjai Pisith2 1 Faculty of Physics, University Vienna, A-1090 Vienna, Austria. Department of Physics, Nanomaterials Research Unit, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Helsinki University Technol, FI-02015 Helsinki, Finland. 4 Advanced Technology Institute, University of Surrey, Guildford GU2 7XH, Surrey, England. 5 NE Normal University, School of Physics, Changchun 130024, People’s Republic of China. 6 BESSY II, D-12489 Berlin, Germany. 2 Abstract A controlled method for the production of tungsten oxide nanobelts through metal oxidation in presence of ethanol is proposed. At the optimal synthesis conditions, up to 20 mu m long vertically aligned ribbon-like structures with a narrow rectangular cross section can be obtained in a tuned manner with preferential formation of WO(2) in the presence of graphitic like carbon. Bulk and local-scale studies suggest that carbon diffusion to the surface of the material leads to the formation of mainly WO(3) nanobelts from simple annealing treatments at 450 degrees C. This represents one alternative method to the common tungsten oxidation in air, opening the possibility to use C-containing compounds with negligible formation of carbide traces. An in-depth characterization of these materials has been performed, and the possible growth mechanisms are here discussed. Key Words: CARBON NANOTUBES; ELECTROCHROMIC DEVICES; NANOWIRES; GROWTH; NANORODS; ARRAYS; VAPOR JOURNAL OF PHYSICAL CHEMISTRY C Volume: 114 Issue: 1 Pages: 10-14. DOI: 10.1021/ jp9085975 Published: JAN 14 2010. 278 Extended X-Ray Absorption Fine Structure and X-Ray Diffraction Studies of Mn-Doped PZN-PZT Ceramics Ngamjarurojana Athipong1,4, Srisombat La-Ongnuan2, Yimnirun Rattikorn3 and Ananta Supon1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 School of Technology, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. 4 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. Abstract In this work, the selected compositions of a combination between perovskite piezoelectric ceramics lead zinc niobate (PZN) and lead zirconate titanate (PZT), close to the morphotropic phase boundary (MPB) i.e. the 0.2PZN-0.8PZT, doped with MnO2 concentrations of 0.0-0.9 wt% were fabricated by a simple solid-state reaction and a pressureless sintering techniques. X-ray diffraction (XRD) spectra from these materials reveal transformation of the tetragonal into the rhombohedral structure. The local structure of Mn was analyzed by mean of synchrotron extended X-ray absorption fine structure (EXAFS) measurements at the Mn K-edge. The correlation between the structural changes and the Mn content was analyzed and compared. The EXAFS analysis indicates that Mn ions should occupy the B-sites in PZN-PZT structure and plays a critical role for the hard ferroelectric behavior of the materials. Key Words: LEAD-ZIRCONATE-TITANATE; ELECTRICAL-PROPERTIES; PIEZOELECTRIC CERAMICS FERROELECTRICS Volume: 405 Pages: 50-56. Article Number: PII 930390286. DOI: 10.1080/00150193.2010.482888. Published: 2010. 279 Fabrication and Electrical Properties of Lead Zirconate Titanate-Cement-Epoxy Composites Rianyoi R.1, Potong R.1, Jaitanong N.1, Yimnirun R.2 and Chaipanich A.1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Institute, Institute of Science, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract In this study, epoxy modified lead zirconate titanate-cement 0-3 and 1-3 composites were fabricated via two different techniques with epoxy added as the third phase. The 0-3 composites were fabricated by the normal paste mixing, while the 1-3 composites were fabricated using a dice-and-fill technique. The dielectric and ferroelectric properties of the composites were examined. The results showed that dielectric constant (epsilon r) at frequency of 1 kHz of 1-3 composites was higher and dielectric loss was lower than those of the 0-3 composite. The ferroelectric hysteresis loops agree with the results showing reduction area in lossy characteristic and the loops become more apparent in 1-3 composites. The use of epoxy results in a lower Pir value but a reduction in the lossy appearance of P-E loops is observed. Key Words: PZT PARTICLE-SIZE; PIEZOELECTRIC PROPERTIES; DIELECTRICPROPERTIES; POLARIZATION; BEHAVIOR FERROELECTRICS Volume: 405, Issuse 1, 2010. Pages: 154-160. DOI: 10.1080/000150193. 2010.483194. Published: 2010. 280 Fabrication and properties of plasma-sprayed Al(2)O(3)/TiO(2) composite coatings: A role of nano-sized TiO(2) addition Dejang N.1, Watcharapasorn A.1, Wirojupatump S.2, Niranatlumpong P.3 and Jiansirisomboon S.1 1 Department of Physics & MMaterials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 National Metal & Materials Technology Center, Pathum Thani 12121, Thailand. Abstract Al(2)O(3)/xTiO(2) (where x = 0, 3, 13 and 20 wt%) composite coatings were deposited onto mild steel substrates by atmospheric plasma spraying of mixed micron-sized Al(2)O(3) and nano-sized TiO(2) powders. Phase transformation from mainly stable alpha-Al(2)O(3) and anatase-TiO(2) in the powders to predominant metastable gamma-Al(2)O(3) and rutile-TiO(2) in the coatings was observed. Reaction between Al(2)O(3) and TiO(2) phase also occurred producing Al(2)TiO(5) phase. Microstructural investigation showed that well separated TiO(2) lamellas were homogeneously dispersed between Al(2)O(3) lamellas. Hardness was found to decrease with increasing TiO(2) content while fracture toughness increased. The average wear rates of composite coatings determined by sliding wear test were lower than that of monolithic Al(2)O(3) coating by approximately 40%. This was mainly attributed to an increase in fracture toughness and reduction of friction coefficient due to a presence of TiO(2) splats. (C) 2009 Elsevier B.V. All rights reserved. Key Words: TITANIA COATINGS; MICROSTRUCTURE; BEHAVIOR; POWDERS; ALUMINA SURFACE & COATINGS TECHNOLOGY Volume: 204 Issue: 9-10 Pages: 1651-1657. DOI: 10.1016/j.surfcoat.2009.10.052. Published: JAN 25 2010. 281 Fallow to forest: Applying indigenous and scientific knowledge of swidden cultivation to tropical forest restoration Wangpakapattanawong Prasit1, Kavinchan Nuttira1, Vaidhayakarn Chawapich1, Schmidt-Vogt Dietrich2 and Elliott Stephen3 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Center of Mountain Ecosystem Studies, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People’s Republic of China. 3 Department of Biology, Faculty of Science, Forest Restoration Ressearch Unit FORRU CMU, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract Rotational swidden cultivation systems, with fallow periods long enough for the regeneration of secondary forests are capable of maintaining forest cover and plant diversity in a dynamic balance in swidden cultivation landscapes. Regeneration of secondary forests through several successional stages and by a combination of coppicing and seedling development is still poorly understood, especially the influence of different swiddening practices and the role of animals as seed dispersers. Swidden cultivators possess a vast knowledge of plants growing in swidden fallows and of fallow dynamics as well. Forest restoration in Thailand has been carried out mainly on the basis of experimental research on the potential of indigenous tree species to promote natural forest regeneration and biodiversity recovery; the so-called framework species. Another viable source of knowledge for forest restoration can be the study of the semi-natural revegetation processes in fallows and the indigenous knowledge of swiddeners of these processes. The research presented here was carried out to attain a better understanding of forest regeneration on fallow swiddens under different swiddening regimes and how it may be applied to practical forest restoration, We investigated the vegetation characteristics of from various stages of secondary succession in fallow swiddens of the Karen and Lawa ethnic groups in the Mae Chaem watershed, Chiang Mai province, northern Thailand. Indigenous knowledge on the use of species and traditional ecological processes in swiddening was recorded by questioning key informants. The data were analyzed and discussed with respect to their application in forest restoration and participatory forest management. (C) 2010 Elsevier B.V. All rights reserved. Key words: Ethnobotany; Northern Thailand; Secondary succession; Swidden cultivation; Traditional knowledge FOREST ECOLOGY AND MANAGEMENT Volume: 260 Issue: 8 Pages: 1399-1406. DOI: 10.1016/j.foreco.2010.07.042 Published: SEP 15 2010. 282 Fast Processing Technique for TiC Coatings on Titanium Boonruang Chatdanai and Thongtem Somchai Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Coatings of TiC on titanium by current heating technique was studied. The titanium rod was cut into the disks with about 20 mm diameter and about 1.5 mm thick. The disks were ground with SiC papers and polished with 0.05 mu m alumina slurry, and then they were cleaned with acetone. In the coating process, titanium disks were placed amidst 20 mm diameter graphite powders pressed against them in the glass tube. The electric current was applied across the graphite matrix with the fixed electric powers from 100 to 180 W for 20 min in argon atmosphere. The titanium disks were characterized using XRD, SEM, EDS and microhardness tester. The coating temperature, mass change, carbon concentration and hardness increased as the applied electric power increased. TiC was detected on the titanium coated at 120 to 180 W. The hardness of the titanium coated at 180 W is the highest at 6.53 GPa. Key Words: PULSED-LASER DEPOSITION; ION-IMPLANTATION; FILMS; CARBON CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 2 Pages: 206-212. Published: MAY 2010. 283 Ferroelectric Hysteresis Behavior of 0-3 PMNT-Cement Composites Jaitanong N.1, Vittayakorn W. C.1, Yimnirun R.2 and Chaipanich A.1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract Lead magnesium niobate titanate (PMNT)Portland cement (PC) composites were produced. PMNT amount used in this work was 50% by volume. The ferroelectric (P-E) hysteresis behaviors were investigated as function of both frequency and electric field for all composites. From the results, it was found that there was an increase in both the instantaneous remnant polarization (Pir) and coercive field (Eic) when the applied field increased from 5 kV/cm to 20kV/cm, Pir was found to increase from 1.5 to 9.8 C/cm2 and Eic was found to increase from 3.4 to 13.8 kV/cm. On the other hand, Pir and Eic were found to reduce from 11.1 to 8.8 C/ cm2 and from 10.8 to 15.3 kV/cm respectively, when the frequency was increased from 30-90 Hz; ascribed to the delayed response of the polarization reversal to the varying external field. Key Words: PZT PARTICLE-SIZE; PIEZOELECTRIC PROPERTIES; DIELECTRICPROPERTIES; POLARIZATION; SYSTEMS FERROELECTRICS Volume: 405. Pages: 105-110 Article Number: PII 930373757. DOI: 10.1080/00150193.2010.482900 Published: 2010. 284 Fifty Three New Record Species of Benthic Diatoms from Mekong River and Its Tributaries in Thailand Suphan Sutthawan and Peerapornpisal Yuwadee Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A study on the diversity of benthic diatoms in Mekong River and its tributaries in Thailand was carried out during July 2005 to April 2007. Two hundred and fifty three species of benthic diatoms were found. Fifty three species have never been previously recorded in Thailand. It could be classified into 3 classes, 11 orders, 22 families and 32 genera. The detailed descriptions of these new records were reported. CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 2 Pages: 326-343. Published: MAY 2010. 285 Five new Mallinella species from the Sundaland of Indonesia (Araneae, Zodariidae) Dankittipakul Pakawin1, Jocque Rudy2 and Singtripop Tippawan1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand 2 Department of African Zoology, Royal Museum for Center Africa, B-3080 Tervuren, Belgium Abstract Five new spider species belonging to the genus Mallinella Strand, 1906 (Araneae, Zodariidae) are described from the Greater Sunda (Sumatra, Borneo, Java) and adjacent smaller islands (Bali, Palau Belitung). These taxa are M. abdita sp. nov. (male female, East Kalimantan, Borneo), M. bifida sp. nov. (male female, East and South Kalimantan, Borneo), M. insulana sp. nov. (male, Bali), and M. wiputrai sp. nov. (male female, Palau Belitung). Males that were considered by Simon to be conspecific with Storena melanognatha van Hasselt, 1882 prove to belong to an unknown species and here described as Mallinella simoni sp. nov. (male, Java and Palau Belitung). New collections from Sundaland confirm that the first species is confined to Sumatra whereas M. simoni sp. nov. is hitherto known from Java and Palau Belitung. The known distribution of Mallinella albomaculata Bosmans & Hillyard, 1990, mentioned from Sulawesi, is here extended to the island of Borneo; the internal epigyne structure of this species is described and illustrated for the first time. Key words: Java; Bali; Sumatra; Kalimantan; Borneo; Palau Belitung; biodiversity; new record; Asceua; Storena ZOOTAXA Issue: 2636 Pages: 21-36 Published: OCT 5 2010. 286 Fixed point theorems for alpha-nonexpansive mappings Klin-eam Chakkrid2,3 and Suantai Suthep1,3 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mathematics, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand. 3 Faculty of Science, PERDO National Center of Excellence in Mathematics, Mahidol University, Bangkok 10400, Thailand. Abstract In this paper, we prove some fixed point theorems of alpha-nonexpansive mappings introduced by Goebel and Pineda (2007) [1]. Moreover, the demiclosedness principle for the a-nonexpansive mappings is also established. (C) 2010 Elsevier Ltd. All rights reserved. Key words: alpha-nonexpansive mapping; Fixed point; Lipschitzian mapping; Contraction APPLIED MATHEMATICS LETTERS Volume: 23 Issue: 6 Pages: 728-731. DOI: 10.1016/j. aml.2010.02.018 Published: JUN 2010. 287 FIXED POINT THEOREMS FOR NONLINEAR MAPPINGS AND STRICT CONVEXITY OF BANACH SPACES Dhompongsa S.1, Fupinwong W.1, Takahashi W.2 and Yao J. C.2 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Applied Mathematics, Natlional Sun Yat Sen University, Kaohsiung 80424, Taiwan. Abstract In this paper, we first prove a fixed point theorem for generalized nonexpansive type mappings in a Banach space by using Kohsaka and Takahashi’s fixed point theorem [10] for nonspreading mappings. Then using Takahashi. Yao and Kohsaka’s result [21], we obtain a necessary and sufficient condition for the existence of fixed points of generalized nonexpansive type mappings. Further, we prove a fixed point theorem for nonspreading mappings with compact domains in a Banach space. Using this result, we give a necessary and sufficient condition for strict convexity of Banach spaces. Key Words: FIRMLY NONEXPANSIVE-MAPPINGS; UNBOUNDED SETS; HILBERTSPACE; PROPERTY JOURNAL OF NONLINEAR AND CONVEX ANALYSIS Volume: 11 Issue: 1. Pages: 175183. Published: 2010. 288 Flame-Spray-Made Undoped Zinc Oxide Films for Gas Sensing Applications Tamaekong Nittaya1, Liewhiran Chaikarn2, Wisitsoraat Anurat3 and Phanichphant Sukon1 1 Department of Chemistry, Faculty of Science, Nanoscience Research Laboratory, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 National Electronics & Computer Technology Center, Pathum Thani 12120, Thailand. Abstract Using zinc naphthenate dissolved in xylene as a precursor undoped ZnO nanopowders were synthesized by the flame spray pyrolysis technique. The average diameter and length of ZnO spherical and hexagonal particles were in the range of 5 to 20 nm, while ZnO nanorods were found to be 5-20 nm wide and 20-40 nm long, under 5/5 (precursor/oxygen) flame conditions. The gas sensitivity of the undoped ZnO nanopowders towards 50 ppm of NO(2), C(2)H(5)OH and SO(2) were found to be 33, 7 and 3, respectively. The sensors showed a great selectivity towards NO(2) at high working temperature (at 300 degrees C), while small resistance variations were observed for C(2)H(5)OH and SO(2), respectively. Key words: undoped ZnO; flame spray pyrolysis; NO(2); C(2)H(5)OH; SO(2); gas sensor SENSORS Volume: 10 Issue: 8 Pages: 7863-7873 DOI: 10.3390/s100807863. Published: AUG 2010. 289 Flow based immuno/bioassay and trends in micro-immuno/biosensors Hartwell Supaporn Kradtap1,2 and Grudpan Kate1,2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The term immuno/bioassay refers to analytical techniques that utilize the specific molecular recognition between antibodies and antigens or between biomolecules and specific receptors. Features such as high selectivity and low sample consumption make them very useful for analysis of samples with complex matrices. However, immuno/bioassays involve time-consuming (multi-step) operations which usually consist of steps of multiple incubation and washing. These are tedious and may result in large errors. Automatted immuno/bioassay systems can ease and shorten these processes and thus are highly beneficial. The hyphenation of flow-based techniques (i.e. flow injection related techniques and micro-fluidic systems) with immuno/bioassay protocols paves a new way for performing such assays. Compared to conventional micro-plate formats, flow-based immuno/bioassays can reduce the time needed for analysis, the volumes of samples and reagents consumed, and the need for trained personnel. In order to transform immuno/ bioassays from conventional to flow-based formats, the solid surfaces used for the immobilization step has to be changed in order to meet the specific requirements of flow systems. To further develop the on-site analytical systems in micro-fluidic platforms, improvements in detection methods are necessary for high-sensitivity and rapid measurement. This review overviews the advantages and disadvantages of flow-based immuno/bioassay formats, the various types of solid surfaces for immobilization, and the methods of detection. Trends to improve sensitivity, speed and robustness are emphasized. Key words: Flow injection; Immunoassay; Bioassay; Sensors MICROCHIMICA ACTA Volume: 169 Issue: 3-4 Pages: 201-220 DOI: 10.1007/s00604-0100333-1. Published: JUN 2010. 290 Flow Injection Determination of Iron Ions with Green Tea Extracts as a Natural Chromogenic Reagent Pinyou Piyanut, Hartwell Supaporn Kradtap, Jakmunee Jaroon, Lapanantnoppakhun Somchai and Grudpan Kate Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A green chemistry system that utilizes natural extract as an alternative reagent for determination of metal ions has been investigated. The extract of green tea, a common beverage, is used for quantification of iron. The green tea extract was prepared in acetate buffer pH 4.8 and could be used without further purification with a simple flow injection (FI) system. The intensity of iron-green tea extract complexes was monitored using a green LED photometer. The use of natural reagent combined with the simple FI-LED photometer set up offers an alternative low cost and rapid system for the determination of metal ions. Detection limit of 0.05 ppm of Fe(III) ion and sampling rate of 180 injections h(-1) were achieved. Application of this system to the analysis of iron in pharmaceutical samples was demonstrated, and the results agreed well with those from the AOAC method. Key Words: CRUDE EXTRACT; POLYPHENOLS; IRON(III); HEALTH; MECHANISMS; CHEMISTRY; KINETICS; OXIDASE ANALYTICAL SCIENCES Volume: 26 Issue: 5 Pages: 619-623 Published: MAY 2010. 291 Flow injection spectrophotometric determination of lead using 1,5-diphenylthiocarbazone in aqueous micellar Ruengsitagoon Wirat1, Chisvert Alberto2 and Liawruangrath Saisunee3,4 1 Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen 40002, Thailand. 2 Department of Analytical Chemistry, University of Valencia, E-46100 Valencia, Spain. 3 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Center of Excellence for Innovation Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A simple flow injection colorimetric procedure for determining lead was established. It is based on the reaction of lead in sulfuric acid with 1,5-diphenylthiocarbazone and sodium dodecyl sulfate, resulting in an intense red-blue complex with a suitable absorption at 500 nm. A standard or sample solution was injected into the sulfuric acid stream (flow rate of 2.0 ml min(-1)), which was then merged with sodium dodecyl sulfate stream (flow rate of 2.0 ml min(-1)) and 1,5-diphenylthiocarbazone stream (flow rate of 1.5 ml min(-1)). Optimum conditions for determining lead were investigated by univariate method. Under the optimum conditions, a linear calibration graph was obtained over the range 1.0-12.01 mu g ml(-1) and the detection limit was 0.027 mu g ml(-1) (s/n=3). The relative standard deviation of the proposed method calculated from 12 replicate injections of 4.0 and 8.0 mu g ml(-1) of lead was 0.42% and 0.38%, respectively. The sample throughput was 80 h(-1). The proposed method has been satisfactorily applied to the determination of lead in water samples. (C) 2010 Elsevier B.V. All rights reserved. Key Words: ATOMIC-ABSORPTION-SPECTROMETRY; PLASMA-MASS SPECTROMETRY; TRACE-LEVEL LEAD; CAPILLARY-ELECTROPHORESIS; SPECIATION ANALYSIS; RAPID-DETERMINATION; SAMPLES; MERCURY; ION; CHROMATOGRAPHY TALANTA Volume: 81 Issue: 1-2 Pages: 709-713. DOI: 10.1016/j.talanta.2010.01.001 Published: APR 15 2010. 292 Frequency dependence of the Ising-hysteresis phase-diagram: Mean field analysis Punya Atchara1, Yimnirun Rattikorn2, Laoratanakul Pitak3 and Laosiritaworn, Yongyut1,4 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. 3 National Metal & Materials Technology Center, Klongluang 12120, Pathumthani, Thailand. 4 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. Abstract In this study, the magnetic hysteresis properties of the Ising model in an oscillating external magnetic field were evaluated using mean field analysis. The average magnetization over a full oscillating field-cycle, as a function of the field amplitude, field frequencies and temperature, was investigated. The dynamic phase transition boundaries between the dynamic ferromagnetic and paramagnetic phases were defined on the field amplitude and temperature plane for each field frequency, in order to study the effect of frequency on the hysteresis dynamic phase transition. For the results, the hysteresis behaviors across the dynamic phase transition boundary were observed using varying field frequencies. The frequency dispersion of the hysteresis area, the remanence and the coercivity were categorized into three distinct types. These phenomena can be explained through an understanding of the role of frequency in the alteration of the dynamic phase boundaries, something which results in anomalous hysteresis properties as a function of frequency. (C) 2010 Elsevier B.V. All rights reserved. Key words: Mean field; Ising model; Hysteresis; Dynamic phase transition PHYSICA B-CONDENSED MATTER Volume: 405 Issue: 16 Pages: 3482-3488, DOI: 10.1016/j.physb.2010.05.028 Published: AUG 15 2010. 293 gamma-Aminobutyric Acid (GABA) Accumulations in Rice During Germination Jannoey Panatda1,2,3, Niamsup Hataichanoke1,2, Lumyong Saisamon4, Tajima Shigeyuki5, Nomura Mika5 and Chairote Griangsak1,2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center for Innovation Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, Faculty of Science & Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, Thailand. 4 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 5 Department of Life Science, Faculty of Agricuture, Kagawa University, Kagawa, Japan. Abstract GABA (gamma-Aminobutyric acid)-enriched rice becomes a popular healthy food nowadays. It has a major inhibitory neurotransmitter function. It inhibits cancer cell proliferation and also reduces blood pressure. GABA concentrations in rice grains and leaves of five well-known rice cultivars were investigated during germination. After germination of whole rice grains, hull and young leaves were removed and grains was used for consumption. Young leaves, waste from rice milling process, and germinated rice grains were collected to determine GABA concentrations by LC-MS after 2-hydroxynaphthaldehyde (HN) derivative formation. Although all of the cultivars have different initial glutamic acid concentrations, GABA concentrations in rice were not different among them (p>0.05). The GABA concentrations in rice grains and leaves were dramatically increased with germination days. However, rice leaves contained more GABA than rice grains by 2-3 folds in all rice cultivars. The highest GABA concentrations in rice grains and young leaves were found at 20 and 30 germination days, respectively. After 20 days of germination, GABA concentrations in rice grains were decreased. GABA concentrations in germinated rice grains were found to be 0.19-1.25 mg/g in Pitsanulok2 (PL2) rice; 294 0.30-2.01 mg/g in Chainat1 (CN1) rice; 0.51-2.45 mg/g in Kawdokmali 105 (KDML 105) rice; 0.34-1.74 mg/g in Supan 1 (SP1) rice and 0.39-1.59 mg/g in Patum1 (PT1) rice cultivars during germination. In contrast, rice leaves showed increased GABA concentrations until 30 germination days. The GABA concentrations were shown to be 1.45-3.14 mg/g, 1.36-2.85 mg/g, 2.39-2.52 mg/g, 0.82-2.09 mg/g and 1.33-1.50 mg/g in normal rice PL2, CN1, KDML 105, SP1 and PT1, respectively. Data of the GABA accumulation and disappearance in rice produced by germination method were presented. These results support effective uses of germinated rice grains for consumption and rice leaves for pharmaceutical application. Key Words: BROWN RICE; GLUTAMATE-DECARBOXYLASE; AMINO-ACIDS; SEEDLINGS; SOAKING CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 1 Pages: 124-133 Published: JAN 2010. 295 Gel-carbon nanotube materials: the relationship between nanotube network connectivity and conductivity Songmee Naratip1,2, Singjai Pisith2 and Panhuis Marc In Het1 1 School of Chemistry, Soft Materials Group, University of Wollongong, Wollongong, NSW, Australia. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Nanomaterials Research Unit, Chiang Mai 50200, Thailand. Abstract The electrical resistance of carbon nanotube networks (NNs) prepared from combinations of gellan gum, xanthan gum, Triton X-100, SWNT and MWNT is reported. It is demonstrated that the NN conductivity can be obtained by analysing the resistance of two overlapping NN as a function of their overlap distance. Unexpectedly, the connectivity between two overlapping NN was found to scale with the electrical conductivity over 4 orders of magnitude. Insights into the dependence of inter-NN contact on applied pressure were obtained. Key Words: EXTRACELLULAR POLYSACCHARIDE; TRANSPARENT; FILMS; ELECTRONICS; GUM; TRANSISTORS; PERCOLATION; COMPOSITES NANOSCALE Volume: 2 Issue: 9 Pages: 1740-1745. DOI: 10.1039/c0nr00259c. Published: 2010. 296 Genetic diversity and community of endophytic actinomycetes within the roots of Aquilaria crassna Pierre ex Lec assessed by Actinomycetes-specific PCR and PCR-DGGE of 16S rRNA gene Nimnoi Pongrawee1, Pongsilp Neelawan2 and Lumyong Saisamorn1 1 Department of Biology, Division Microbiology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand. Abstract PCR denaturing gradient gel electrophoresis (DGGE) was used to determine diversity and community of endophytic actinomycetes distributed within the roots of Aquilaria crassna Pierre ex Lec (eaglewood) DNA was extracted from plant roots collected from one plantation in Nakhonnayok province and three plantations in Phetchabun province of Thailand A nested-PCR was used to specifically amplify all actinobacterial groups PCR-DGGE analysis of a variable region 3 (V3) of 16S rDNA confirmed the presence of endophytic actinomycetes in genera Nocardia Pseudonocardia Streptomyces and Actinomadura within the roots of eaglewood from Phetchabun province Actinomycetes in genera Nocardta Nonomuraea Pseudonocardia and Actinomadura were found to inhabit abundantly in the roots of eaglewood from Nakhonnayok province Actinobacterial community structures within the roots of this plant grown in two provinces were different from each other based on the generated dendrogram and Sorensen s index These results suggest that different locations resulted in different endophytic actinomycetes communities within the plant Besides actinobacterial community structure genetic diversity was analyzed based on species diversity and simple index DGGE exhibited many species of actinomycetes inhibited as endophytes The highest diversity of endophytic actinomycetes was found in the roots nom a plantation in Nakhonnayok province and one of the plantations in Phetchabun province This is the first report of the ecology and the community of endophytic actinomycetes colonized and imbedded within the roots of eaglewood plant (C) 2010 Elsevier Ltd All rights reserved Key words: PCR DGGE; Endophytic actinomycetes; Community; Diversity BIOCHEMICAL SYSTEMATICS AND ECOLOGY Volume: 38 Issue: 4 Pages: 595-601. DOI: 10.1016/j.bse.2010.07.005. Published: AUG 2010. 297 Genetic evidence supports linguistic affinity of Mlabri-a hunter-gatherer group in Thailand Xu Shuhua1,2, Kangwanpong Daoroong3, Seielstad Mark4, Srikummool Metawee3, Kampuansai Jatupol3 and Jin Li1,2,5,6,7,8 1 Chinese Academy of Sciences, Chinese Academy Sciences & Max Planck Society CAS MPG Partner, Shanghai Institute for Biology Sciences, Shanghai 200031, People’s Republic of China. 2 Chinese Academy of Sciences, CAS MPG Partner Institute for Computational Biology, Key Lab of Computational Biology, Shanghai 200031, People’s Republic of China. 3 Department of Biology, Faculty of Sciences, Chiang Mai University, Chiang Mai 50202, Thailand. 4 Genome Institute of Singapore, Singapore 138672, Singapore. 5 State Key Lab of Genetic Engineering, Fudan University, Shanghai 200433, People’s Republic of China. 6 Minitry of Education, Key Lab of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200433, People’s Republic of China. 7 Institute Biomedioal Sciences, Fudan University, Shanghai 200433, People’s Republic of China. 8 Institute of Health Sceinces, Taizhou 225300, Jiangsu, People’s Republic of China. Abstract Background: The Mlabri are a group of nomadic hunter-gatherers inhabiting the rural highlands of Thailand. Little is known about the origins of the Mlabri and linguistic evidence suggests that the present-day Mlabri language most likely arose from Tin, a Khmuic language in the Austro-Asiatic language family. This study aims to examine whether the genetic affinity of the Mlabri is consistent with this linguistic relationship, and to further explore the origins of this enigmatic population. Results: We conducted a genome-wide analysis of genetic variation using more than fifty thousand single nucleotide polymorphisms (SNPs) typed in thirteen population samples from Thailand, including the Mlabri, Htin and neighboring populations of the Northern Highlands, speaking Austro-Asiatic, TaiKadai and Hmong-Mien languages. The Mlabri population showed higher LD and lower haplotype diversity when compared with its neighboring populations. Both model-free and Bayesian model-based clustering analyses indicated a close genetic relationship between the Mlabri and the Htin, a group speaking a Tin language. Conclusion: Our results strongly suggested that the Mlabri share more recent 298 common ancestry with the Htin. We thus provided, to our knowledge, the first genetic evidence that supports the linguistic affinity of Mlabri, and this association between linguistic and genetic classifications could reflect the same past population processes. Key Words: MAXIMUM-LIKELIHOOD-ESTIMATION; MULTILOCUS GENOTYPE DATA; HUMAN GENOME; LANGUAGE REPLACEMENT; TESTING HYPOTHESES; HUMANPOPULATIONS; HAPLOTYPE MAP; Y-CHROMOSOME; ADMIXTURE; EUROPE BMC GENETICS Volume: 11 Article Number: 18. DOI: 10.1186/1471-2156-11-18. Published: MAR 19 2010. 299 Graft polymerization of flame-retardant compound onto silk via plasma jet Chaiwong C.1,2, Tunma S.1,2, Sangprasert W.3, Nimmanpipug P.3 and Boonyawan D.1,2 1 Department of Physics & Materials Science, Plasma & Beam Physics Research Facil, Faculty of Science, Chiang Mai Univeristy, Chiang Mai 50200, Thailand. 2 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 3 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract We investigated the application of an atmospheric pressure plasma jet to impart flame retardancy to silk fabrics. Argon plasma jet was used to graft nondurable phosphorus-based flame retardant agent (Pyrovatim (R) PBS) onto silk. The treated fabric showed a higher level of flame retardancy as evaluated by using 45 degrees flammability test. To evaluate the durability, the treated silk was submitted to washing process. It was found that Ar plasma conferred durable flame retardancy to the treated silk fabric since the flame retardant character retained after the washing process. Scanning electron microscopy revealed that the yarn of the Ar treated silk was uniformly covered with the PBS particles whereas PBS deposited locally on the untreated silk. Energy dispersive X-ray spectroscopy showed the presence of phosphorus up to 11 wt.% in the Ar treated silk. Fourier transform infrared spectroscopy exhibited the bondings between phosphorus and the silk molecular chains. The molecular dynamics simulations affirmed the incorporation of phosphorus in the structure of silk. (C) 2010 Elsevier B.V. All rights reserved. Key words: Silk; Flame retardancy; Plasma jet; Graft polymerization; MD simulations SURFACE & COATINGS TECHNOLOGY Volume: 204 Issue: 18-19. Pages: 2991-2995 DOI: 10.1016/j.surfcoat.2010.02.044. Published: JUN 25 2010. 300 Grain growth behavior in bismuth titanate-based ceramics Watcharapasorn A., Siriprapa P. and Jiansirisomboon S. Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Abstract Bismuth titanate and lanthanum-doped bismuth titanate ceramics were prepared from freeze-dried powders employing conventional solid state reaction and sintering procedures. The sintering process was carried out at 1150 degrees C from 4 up to 48 h. X-ray diffraction analysis showed that preferred orientation was reduced in bismuth titanate ceramic as sintering time increased while lanthanum-doped sample showed much less degree of preferred orientation and was independent of sintering time. Grain growth studies also showed that initial anisotropic grain growth rate was the main factor controlling the grain morphology, rendering the plate-shaped grain in both pure and lanthanum-doped bismuth titanate ceramics. Based on established grain growth law, pore-controlled diffusion could be the major mechanism determining the observed microstructure in these layered compounds. (C) 2009 Elsevier Ltd. All rights reserved. Key Words: DOPED BI4TI3O12 CERAMICS; SOL-GEL METHOD; FERROELECTRIC PROPERTIES; MAGNETIC ALIGNMENT; CRYSTAL-STRUCTURES; POWDERS; FILMS JOURNAL OF THE EUROPEAN CERAMIC SOCIETY Volume: 30 Issue: 1 Pages: 87-93. DOI: 10.1016/j.jeurceramsoc.2009.07.031. Published: JAN 2010. 301 haracteristics of Trapiche Blue Sapphires from Southern Vietnam Khotchanin Kanyarat, Thanasuthipitak Panjawan and Thanasuthipitak Theerapongs Department of Geological Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Trapiche patterns are common in emeralds but less common in corundum, and are typically restricted to ruby. Trapiche sapphire is comparatively rare. Blue sapphires of the trapiche type from southern Vietnam reveal a distinct core and fixed six-rayed star, similar in appearance to the patterns found in some rubies from Mong Hsu, Myanmar. The trapiche consists of dark brown to brown core, six blue sectors, separated by six brown to brownish yellow arms of the star. Some samples also show a distinct colour zoning. A massive concentration of needle-like inclusions is seen in the arms under the microscope. These are oriented perpendicular to the hexagonal crystal faces. A number of inclusions are also observed in the core area. Gemmological, microscopic, spectroscopic, and chemical properties are presented to describe the characteristics of the blue sapphires from southern Vietnam. CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 1 Pages: 64-73. Published: JAN 2010. 302 Harmonic Analysis of Dynamic Hysteresis Respons of BaTiO3 Bulk Ceramics Kanchiang K.1,2, Yimnirun R.3, Wongdamnern N.1, Ngamjarurojana A.1,2 and Laosiritaworn Y.1,2,4 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 3 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. 4 Science & Technology Reseach Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In this work, the dynamic ferroelectric hysteresis properties in response to dynamic electric field of BaTiO3 bulk ceramics was investigated using the harmonic analysis approach. Fourier transformation was used to analyze the periodic polarization signal on frequency domain via each discrete harmonic. From the results, the hysteresis area is found to depend only on the first harmonic of the real part. On the other hand, the remnant polarization depends on all odd harmonics of the real part. Further, the coercive field can be found from the phase-lag between the inverse Fourier signals re-calculated from the first harmonic of the real part and that of the imaginary part. The hysteresis properties from the harmonic analysis match well with those of the original measurement. This suggests that the harmonic analysis is one of the powerful techniques which can be used to predict hysteresis behavior. Key Words: AC FERROELECTRICS Volume: 401 Pages: 123-128. Article Number: PII 930404371. DOI: 10.1080/00150191003672792. Published: 2010. 303 High-performance thin-layer chromatographic determination of ketoconazole in pharmaceutical formulations Saysin Suwanna1, Liawruangrath Boonsom1 and Liawruangrath Saisunee2,3 1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Center for Innovation in Chemistry, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Institute of Science & Technology Research & Development, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A high-performance thin-layer chromatographic method was developed for the determination of ketoconazole. The sample was separated on a silica gel 60 F(254) plate and developed in ethanol-acetone-1.0 mol l(-1) H(2)SO(4) by means of an automatic multiple-development system. The area of the spot was quantified by a TLC scanner at a wavelength of 298 nm. A linear calibration curve was established over the range of 3-20 mu g/ml of ketoconazole, with a correlation coefficient of 0.9992. The relative standard deviations for intraday and interday precisions, for three replicate determinations, were found to be 1.72% and 0.69% for 5 mu g/ml and 2.18% and 0.94% for 10 mu g/ml of ketoconazole, respectively. The average percentage recoveries of ketoconazole shampoos (Nora, Kenalyn, and Nizoral) and ketoconazole creams (Nizoral, Fungasin, and Ketazon) were found to be 96.10, 97.06, and 99.58, and 96.77, 97.26, and 95.74, respectively. This method has been applied to the determination of ketoconazole in various pharmaceutical dosage forms. Common excipients in formulations do not interfere. This method is simple, precise, accurate, and inexpensive. It should be used for routine analysis. Key Words: LIQUID-CHROMATOGRAPHY; ANTIFUNGAL DRUGS; HUMAN-PLASMA; CLOTRIMAZOLE; SEPARATION; HPLC; FORMALDEHYDE; VALIDATION; SHAMPOO; AGENT JOURNAL OF COSMETIC SCIENCE Volume: 61 Issue: 5 Pages: 367-376. Published: SEP-OCT 2010. 304 HRTEM and ADF-STEM of precipitates at peak-ageing in cast A356 aluminium alloy Chomsaeng N.1, Haruta M.2, Chairuangsri T.3, Kurata H.2, Isoda S.2 and Shiojiri M.4 1 Chiang Mai University, Faculty of Science, Department of Physics, Chiang Mai 50200, Thailand. 2 Kyoto University, Institute for Chemical Research, Kyoto 6110011, Japan. 3 Chiang Mai University, Faculty of Science, Department of Industrial Chemistry, Chiang Mai 50200, Thailand. 4 Kyoto Institute Technology, Kyoto 6180091, Japan. Abstract Precipitates at peak-ageing in an A356 Al-Mg-Si alloy cast by a semi-solid process have been studied by high-resolution transmission electron microscopy (HRTEM) and annular dark-field scanning transmission electron microscopy (ADFSTEM). The major precipitate (ppt) at peak-ageing is the monoclinic beta “coincide or pre-beta”. Its orientation relationship with the fcc-Al matrix is [0 0 1](Al)//[0 1 0](ppt), (0 2 0)(Al)(coincide)//(6 0 1)(ppt) and (2 0 0)(Al)(coincide)//((4) over bar 0 3)(ppt), equivalent to [0 0 1](Al)//[0 1 0](ppt), ((1) over bar 3 0)(Al)//(1 0 0)(ppt) and (3 2 0)(Al)//(0 0 1)(ppt). The habit direction of this precipitate is [0 0 1](Al)// [0 1 0](ppt) forming facet planes on ((1) over bar 3 0)(Al)//(1 0 0)(ppt) and (3 2 0) (Al)//(0 0 1)(ppt), in which very good atomic matching was found. The contrast of the precipitate in ADF-STEM is reversed to the typical atomic number contrast. To understand such contrast, a dynamical simulation based on the multi-slice method was therefore performed using different atomic stacking models and successfully explain the reverse contrast of the experimental images of the precipitate. (C) 2010 Elsevier B.V. All rights reserved. Key Words: MG-SI ALLOYS; TRANSMISSION ELECTRON-MICROSCOPY; CRYSTALSTRUCTURE; BETA’’-PHASE; AL; SYSTEM; ZONES; INTERFACES JOURNAL OF ALLOYS AND COMPOUNDS. Volume: 496 Issue: 1-2. Pages: 478-487 DOI: 10.1016/j.jallcom.2010.02.084. Published: APR 30 2010. 305 Hydrothermal synthesis of double sheaf-like Sb(2)S(3) using copolymer as a crystal splitting agent Pilapong Chalermchai1, Thongtem Titipun1 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Double-sheaves of antimony sulfide were synthesized by the 200 degrees C and 24 h hydrothermal reaction in the acidic solution, containing copolymer-a crystal splitting agent. Using X-ray technique, electron microscopy, and Raman as well as UV-visible NIR absorption spectroscopy, the products were specified as orthorhombic Sb(2)S(3), composing of Sb and S with five vibration modes, in the shape of spears in bundles with the [001] growth direction and 2.38 eV direct energy gap. A diffraction pattern was also simulated, and is in good accordance with that obtained by the interpretation. (C) 2010 Elsevier B.V. All rights reserved. Key Words: GROWTH JOURNAL OF ALLOYS AND COMPOUNDS. Volume: 507 Issue: 2 Pages: L38-L42 DOI: 10.1016/j.jallcom.2010.08.003. Published: OCT 8 2010. 306 Hydrothermal Synthesis of Lead Titanate Fine Powders at Water Boiling Temperature Chankaew Chaiyos1,2 and Rujiwatra Apinpus1,2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Ministry of Education, Commission on Highter Education, Center of Excellence Innovation in Chemistry, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Lead titanate fine powders comprising of a single tetragonal phase and well characterized particles were successfully synthesized at an exceptionally low temperature of 100 degrees C. The important role of the ultrasonication in reducing the thermodynamic barrier of the hydrothermal reaction, and the slow rare of hydrothermal reaction at 100 degrees C were underlined. The synthesized particles were revealed to be tetragonal in shape, mostly submicrometers, in size, and generally present as large aggregates. The layer-by-layer model was also proposed as the particle growth mechanism. Key Words: PHASE-FORMATION; NANOPOWDERS CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 1 Pages: 92-98. Published: JAN 2010. 307 Hydrothermal synthesis of monoclinic WO(3) nanoplates and nanorods used as an electrocatalyst for hydrogen evolution reactions from water Ham Dong Jin2, Phuruangrat Anukorn1,2, Thongtem Somchai1 and Lee Jae Sung2 1 Department of Physics & Materials Science, Faculty of Sceince, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Pohang University of Science & Technology, School of Environmental Science & Engineering, Department of Chemical Engineering, Eco Friendly Catalysis & Energy Laboratory NRL, Pohang 790784, South Korea. Abstract Monoclinic WO(3) (m-WO(3)) nanoplates and nanorods were successfully synthesized by a simple hydrothermal process using sodium tungstate dihydrate (Na(2)WO(4) 2H(2)O) ammonium nitrate (NH(4)NO(3)) and polyethylene glycol (PEG) as initial precursors Phase morphologies and electrochemical properties of the products were characterized by X-ray diffraction (XRD) scanning and transmission electron microscopy (SEM TEM) high-resolution transmission electron microscopy (HRTEM) cyclic voltammetry (CV) and linear sweep voltammetry (LSV) The effect of NH(4)NO(3) concentration on the formation of the pure phase of m-WO(3) nanomaterial was studied The product synthesized under NH(4)NO(3)-free condition was pure orthorhombic WO(3) 0 33H(2)O (o-WO(3) 0 33H(2)O) phase By adding and increasing the amount of NH(4)NO(3) to the solution m-WO(3) phase started to form and became pure m-WO(3) phase when 1 50 g NH(4)NO(3) was used The morphology of m-WO(3) was nanoplates and became nanorods by PEG adding The nanostructured m-WO(3) showed much higher electrocatalytic activity for hydrogen evolution from water than that of the commercial bulk m-WO(3) including the m-WO(3) nanorods with slightly better than the m-WO(3) nanoplates (C) 2010 Elsevier B V All rights reserved Key Words: TUNGSTEN-OXIDE; NANOWIRES; GROWTH; ROUTE CHEMICAL ENGINEERING JOURNAL Volume: 165 Issue: 1 Pages: 365-369. DOI: 10.1016/j.cej.2010.09.003. Published: NOV 15 2010. 308 Hysteresis scaling relations in polycrystalline BaTiO(3) bulk ceramics Wongdamnern N.1, Tangsritragul J.2, Ngamjarurojana A.1, Ananta S.1, Laosiritaworn Y.1 and Yimnirun R.3 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics, Faculty of Science & Technology, Thammasat University, Pathum Thani 12121, Thailand. 3 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract The scaling behaviors of the dynamic ferroelectric hysteresis of polycrystalline BaTiO(3) bulk ceramics were investigated. To enhance the accuracy of prediction, not only sets of the scaling relation of hysteresis area < A > against frequency integral and field amplitude E(0) were established, but also were sets of scaling relation of remnant polarization (P(r)) and coercive field (E(c)) against frequency integral and field amplitude E(0). All scaling relations of hysteresis parameters, (A), Pr, and Ec, displayed obviously the inter-correlation between integral- and E(0)exponents in both sub-coercive field and above coercive field conditions. By first approximation, the scaling relations took a form of < A > alpha integral(-0.39)E(0) (1.06), P(r) alpha integral(-184)E(0)(0.47), and E(c) alpha integral(-0.33)E(0)(0.46) above the coercive field condition: whereas the scaling relations in the form of < A > alpha integral(-0.55)E(0)(3.40), P(r) alpha integral(-0.43)E(0)(1.73), and E(c) alpha integral(-0.27)E(0)(1.35) were obtained under a sub-coercive field condition. The frequency observation range affected directly the scaling exponents. Over the same frequency range, the scaling relations obtained in this study are generally comparable to previously reported scaling behavior in BaTiO(3) single crystals, as well as in other polycrystalline bulk ceramics, suggesting that materials (both single and polycrystalline) with similar domain switching mechanisms should have comparable dynamic hysteresis and scaling behavior. 2010 Elsevier By. All rights reserved. Key Words: SRBI2TA2O9 THIN-FILMS; FERROELECTRIC SRBI2TA2O9; DYNAMIC HYSTERESIS; FREQUENCY-RESPONSE; SPIN SYSTEMS; DISPERSION; BEHAVIOR; MODEL; TEMPERATURE; LOOPS MATERIALS CHEMISTRY AND PHYSICS Volume: 124 Issue: 1 Pages: 281-286. DOI: 10.1016/j.matchemphys.2010.06.032. Published: NOV 1 2010. 309 Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities Thitilertdecha Nont1, Teerawutgulrag Aphiwat2, Kilburn Jeremy D.3 and Rakariyatham Nuansri2 1 Division Biotechnology, Graduated School, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 School of Chemistry, University of Southampton, Southampton SO17 1BJ, Hants, England. 2 Abstract Nephelium lappaceum is a tropical fruit whose peel possesses antioxidant properties. Experiments on the isolation and identification of the active constituents were conducted, and on their antioxidant activity using a lipid peroxidation inhibition assay. The methanolic extract of N. lappaceum peels exhibited strong antioxidant properties. Sephadex LH-20 chromatography was utilized in the isolation of each constituent and the antioxidant properties of each was studied. The isolated compounds were identified as ellagic acid (EA) (1), corilagin (2) and geraniin (3). These compounds accounted for 69.3% of methanolic extract, with geraniin (56.8%) as the major component, and exhibited much greater antioxidant activities than BHT in both lipid peroxidation (77-186 fold) and DPPH(center dot) (42-87 fold) assays. The results suggest that the isolated ellagitannins, as the principal components of rambutan peels, could be further utilized as both a medicine and in the food industry. Key Words: MODELS IN-VITRO; ELLAGIC ACID; HYDROLYZABLE TANNIN; PHYLLANTHUS-AMARUS; PELARGONIUM-RENIFORME; LIPID-PEROXIDATION; EXTRACTS; GERANIIN; ELLAGITANNINS; URINARIA MOLECULES Volume: 15 Issue: 3 Pages: 1453-1465. DOI: 10.3390/molecules15031453. Published: MAR 2010. 310 Immunoproteomic Assay of Streptococcus suis Serotype 2 Isolated from Patients Wongsawan Kanruethai1, Wongpia Aphinya2, Lomthaisong Khemika3, Boonthum Anuson1, Supjatura Volaluck1 and Thraravichitkul Prasit1 1 Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. Abstract Antigenic proteins of Streptococcus suis serotype 2 was investigated using an immunoproteomic technique. The whole cell proteins of two Streptococcus suis strains, LPH02 and MNCM43, isolated from patients with severe toxic shock syndrome and endocarditis were separated by two-dimensional gel electrophoresis (2-DE). Proteins were blotted onto nitrocellulose membrane. Immunodetection was done by individually incubating the membrane with either human serum from infected S. suis patients suffering from meningitis and septic shock or immunized rabbit serum. After excluding antigenic proteins found in serum of patients infected with closely related microorganisms Streptococcus spp., Staphylococcus spp. and Enterococcus spp., a total of 29 and 30 antigenic protein spots of LPH02 and MNCM43 strains were found, respectively. To compare the antigenicity between LPH02 and MNCM43 strains, the number of antigenic protein spots found by reacting with the same serum was considered. The antigenic protein patterns from different patient sera were also compared. Three specific antigenic protein spots were successfully identified by matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-TOF-MS), which matched to o-acetylserine lyase, phosphomannose isomerase, and acyl-ACP thioesterase, respectively. Key Words: GROUP-A STREPTOCOCCUS; TOXIC-SHOCK-SYNDROME; PHOSPHOMANNOSE ISOMERASE; HELICOBACTER-PYLORI; SURFACE PROTEIN; IDENTIFICATION; TYPE-2; GENE; MENINGITIS; STRAINS CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 1 Pages: 134-150. Published: JAN 2010. 311 Impedance study of giant dielectric permittivity in BaFe(0.5)Nb(0.5)O(3) perovskite ceramic Intatha Uraiwan2, Eitssayeam Sukum1, Wang John3 and Tunkasiri Tawee1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50202, Thailand. 2 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. 3 Department of Materials Science & Engineering, Faculty of Engineering, National University Singapore, Singapore 117576, Singapore. Abstract Single-phase BaFe(0.5)Nb(0.5)O(3) (BFN) ceramics were prepared by solidstate reaction method and were chatacterized by X-ray Diffraction (XRD) technique. Then, impedance spectroscopy measurements were conducted in a frequency range from 100 Hz to I MHz and in a temperature range from 293 to 600 K. Relaxation phenomena of non-Debye type have been observed in the BFN ceramics, as confirmed by the Cole-Cole plots. The higher values of epsilon’ at the lower frequencies are explained on the basis of the Maxwell-Wagner (MW) polarization model. Complex impedance analysis enables us to separate the contributions from grains and grain boundaries Of Our samples. We found that at higher temperatures grain boundary resistance is higher than grain resistance, irrespective of composition. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved. Key Words: DISPERSION; TEMPERATURE; ABSORPTION; SR CURRENT APPLIED PHYSICS Volume: 10 Issue: 1 Pages: 21-25. DOI: 10.1016/j. cap.2009.04.006. Published: JAN 2010. 312 In vitro cultivation and fruit body formation of the black bolete, Phlebopus portentosus, a popular edible ectomycorrhizal fungus in Thailand Sanmee Rarunee1, Lumyong Pipob2, Dell Bernard3 and Lumyong Saisamorn1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. 3 School of Biological Science & Biotechnology, Murdoch University, Perth, WA, Australia. 2 Abstract The tropical black bolete Phlebopus portentosus is highly favored in the cuisine of northern Thailand. It is suspected to form ectomycorrhizae with many host trees. Mycelium of P. portentosus isolated from a basidiome in Chiang Rai Province in 2003 grew well on modified Gamborg, modified Melin-Norkans, and Murashige and Skoog media at 30A degrees C and at pH 4. In vitro fructification of P. portentosus on sorghum grain medium without a host plant is presented for the first time. Basidiomes emerged 3 months after inoculation on the medium, and the produced basidiospores germinated on agar, indicating the completion of its life cycle in vitro without a host. Five putative host plants (Castanopsis tribuloides, Dipterocarpus alatus, Dimocarpus longan, Pinus kesiya, and Syzygium cumini) were inoculated with mycelium on sorghum grain medium in a greenhouse to confirm its ectomycorrhizal status. Ectomycorrhizal roots were observed only on Pinus kesiya, suggesting that P. portentosus may be facultatively ectomycorrhizal. Identification of the synthesized ectomycorrhizae was confirmed by PCR amplification of ITS with a designed specific primer (HAR2A). Key Words: ELECTRON MICROSCOPY; IDENTIFICATION; RUBINELLUS; CULTURE; ROOTS MYCOSCIENCE Volume: 51 Issue: 1 Pages: 15-22 DOI: 10.1007/s10267-009-0010-6. Published: JAN 2010. 313 Infections of Nosema ceranae in four different honeybee species Chaimanee Veeranan1, Warrit Natapot2,3 and Chantawannakul Panuwan1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Biology, Faculty of Science, Center of Excellence Entomology, Chulalongkorn University, Bangkok 10330, Thailand. 3 Department of Entomology, National Museum Natural History, Smithsonian Institute, Washington, DC 20013 USA. 2 Abstract The microsporidium Nosema ceranae is detected in honeybees in Thailand for the first time. This endoparasite has recently been reported to infect most Apis mellifera honeybee colonies in Europe, the US, and parts of Asia, and is suspected to have displaced the endemic endoparasite species, Nosema apis, from the western A. mellifera. We collected and identified species of microsporidia from the European honeybee (A. mellifera), the cavity nesting Asian honeybee (Apis cerana), the dwarf Asian honeybee (Apis florea) and the giant Asian honeybee (Apis dorsata) from colonies in Northern Thailand. We used multiplex PCR technique with two pairs of primers to differentiate N. ceranae from N. apis. From 80 A. mellifera samples, 62 (77.5%) were positively identified for the presence of the N. ceranae. Amongst 46 feral colonies of Asian honeybees (A. cerana, A. florea and A. dorsata) examined for Nosema infections, only N. ceranae could be detected. No N. apis was found in our samples. N. ceranae is found to be the only microsporidium infesting honeybees in Thailand. Moreover, we found the frequencies of N. ceranae infection in native bees to be less than that of A. mellifera. (C) 2010 Elsevier Inc. All rights reserved. Key words: Apis mellifera; Asian honeybee; Nosema ceranae; Nosema apis; Microsporidia JOURNAL OF INVERTEBRATE PATHOLOGY Volume: 105 Issue: 2 Pages: 207-210. DOI: 10.1016/j.jip.2010.06.005 Published: OCT 2010. 314 Influence of B(2)O(3) on electrical properties and phase transition of lead-free Ba(Ti(0.9)Sn(0.1))O(3) ceramics Tawichai N.1, Intatha U.2, Eitssayeam S.1, Pengpat K.1, Rujijanagul G.1 and Tunkasiri T.1 1 Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. School of Science, Mae Fah Luang University, Chiang Rai 57000, Thailand. 2 Abstract The phase transition and electrical properties of Ba(Ti0.9Sn0.1)O3 ceramics with B2O3 added were investigated to explore the effect of B2O3 addition on enhanced densification and dielectric constant of these ceramics. With increasing B2O3 content, a linear reduction of ferroelectric to paraelectric transition temperature was observed. In addition, higher B2O3 concentrations enhanced a ferroelectric relaxor behavior in the ceramics. Changes in this behavior were related to densification, second-phase formation and compositional variation of the ceramics. Key Words: SOL-GEL PROCESS; DIELECTRIC-PROPERTIES; BARIUM-TITANATE; FERROELECTRIC PROPERTIES; BA0.7SR0.3TIO3 CERAMICS; THIN-FILMS; BEHAVIOR; FABRICATION; ADDITIONS; BOUNDARY PHASE TRANSITIONS Volume: 83 Issue: 1 Pages: 55-63. Article Number: PII 918913209. DOI: 10.1080/01411590903549005. Published: 2010. 315 Influence of metal cofactors and water on the catalytic mechanism of creatininase-creatinine in aqueous solution from molecular dynamics simulation and quantum study Lee Vannajan Sanghiran1,2, Kodchakorn Kanchanok3, Jitonnom Jitrayut1,2, Nimmanpipug Piyarat1,2, Kongtawelert Prachya3 and Premanode Bhusana4 1 Department of Chemistry, CSML, Chiang Mai University, Chiang Mai 50200, Thailand. Center Innovation Chemistry, Faculty of Science, Thailand Center Excellence for Physics ThEP, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Biochemistry, Faculty of Medicine, Thailand Excellence Center Tissue Engneering & Stem Cells, Chiang Mai University, Chiang Mai 50200, Thailand. 4 University London Imperial College of Science Technology & Medicine, Institute Biomedical Engineering, London, England. 2 Abstract The reaction mechanism of creatinine-creatininase binding to form creatine as a final product has been investigated by using a combined ab initio quantum mechanical/molecular mechanical approach and classical molecular dynamics (MD) simulations. In MD simulations, an X-ray crystal structure of the creatininase/ creatinine was modified for creatininase/creatinine complexes and the MD simulations were run for free creatininase and creatinine in water. MD results reveal that two X-ray water molecules can be retained in the active site as catalytic water. The binding free energy from Molecular Mechanics Poisson-Boltzmann Surface Area calculation predicted the strong binding of creatinine with Zn(2+), Asp45 and Glu183. Two step mechanisms via Mn(2+)/Zn(2+) (as in X-ray structure) and Zn(2+)/Zn(2+) were proposed for water adding step and ring opening step with two catalytic waters. The pathway using synchronous transit methods with local density approximations with PWC functional for the fragment in the active region were obtained. Preferable pathway Zn(2+)/Zn(2+) was observed due to lower activation energy in water adding step. The calculated energy in the second step for both systems were comparable with the barrier of 26.03 and 24.44 kcal/mol for Mn(2+)/Zn(2+) and Zn(2+)/Zn(2+), respectively. Key words: Creatininase-creatinine; Catalytic mechanism; Molecular dynamics simulation; Quantum mechanics JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN Volume: 24 Issue: 10 Pages: 879-886. DOI: 10.1007/s10822-010-9380-2. Published: OCT 2010. 316 Interfacial morphology and domain configurations in 0-3 PZT-Portland cement composites Jaitanong N.1, Zeng H. R.2, Li G. R.2, Yin Q. R.2, Vittayakorn W. C.1, Yimnirun R.3 and Chaipanich A.1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Shanghai Institute of Ceramics, Key Laboratory of Inorganic Functional Materials & Integrated Devices, Chinese Academy of Science, Shanghai 200050, People’s Republic of China. 3 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract Cement-based piezoelectric composites have attracted great attention recently due to their promising applications as sensors in smart structures. Lead zirconate titanate (PZT) and Portland cement (PC) composite were fabricated using 60% of PZT by volume. Scanning Electron Microscope and piezoresponse force microscope were used to investigate the morphology and domain configurations at the interfacial zone of PZT-Portland cement composites. Angular PZT ceramic grains were found to bind well with the cement matrix. The submicro-scale domains were clearly observed by piezoresponse force microscope at the interfacial regions between the piezoelectric PZT phase and Portland cement phase, and are clearer than the images obtained for pure PZT. This is thought to be due to the applied internal stress of cement to the PZT ceramic particle which resulted to clearer images. (C) 2009 Elsevier B. V. All rights reserved. Key Words: SCANNING FORCE MICROSCOPY; PB(MG1/3NB2/3)O-3-PBTIO3 SINGLE-CRYSTALS; FERROELECTRIC THIN-FILMS; PIEZOELECTRIC PROPERTIES; DIELECTRIC-PROPERTIES; SMART MATERIALS; PARTICLE-SIZE; BEHAVIOR; MICROSTRUCTURE; PIEZORESPONSE APPLIED SURFACE SCIENCE Volume: 256 Issue: 10 Pages: 3245-3248. DOI: 10.1016/j. apsusc.2009.12.013 Published: MAR 1 2010. 317 Ishikawa Iterative Process for a Pair of Single-valued and Multivalued Nonexpansive Mappings in Banach Spaces Sokhuma K.2 and Kaewkhao A.1 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mathematics, Faculty of Science, Burapha University, Chon Buri 20131, Thailand. Abstract Let E be a nonempty compact convex subset of a uniformly convex Banach space X, and let t : E -> E and T : E -> KC(E) be a single-valued nonexpansive mapping and a multivalued nonexpansive mapping, respectively. Assume in addition that Fix(t) boolean AND Fix(T) not equal phi and Tw = {w} for all w is an element of Fix(t) boolean AND Fix(T). We prove that the sequence of the modified Ishikawa iteration method generated from an arbitrary x(0) is an element of E by y(n) = (1 - beta(n))x(n) + beta(n)z(n), x(n+1) = (1 - alpha(n))x(n) + alpha(n) ty(n) where z(n) is an element of Tx(n) and {alpha(n)}, {beta n} are sequences of positive numbers satisfying 0 < alpha <= alpha(n), beta(n) <= b < 1, converges strongly to a common fixed point of t and T; that is, there exists x. E such that x = t(x) is an element of T(x). Key Words: FIXED-POINTS; CONVERGENCE; MANN FIXED POINT THEORY AND APPLICATIONS. Article Number: 618767. DOI: 10.1155/ 2010/618767. Published: 2010. 318 Large-scale synthesis of CuS hexaplates in mixed solvents using a solvothermal method Thongtem Titipun1, Pilapong Chalermchai1 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Large-scale covellite CuS hexaplates were successfully synthesized by the 200 degrees C solvothermal reactions of CuCl(2)center dot 2H(2)O and (NH(4)) (2)S in C(2)H(5)OH-H(2)O mixed solvents containing HCOOH as a pH stabilizer, including different amounts and molecular weights (MWs) of polyethylene glycol (PEG). By using XRD and SAED. CuS (hcp) was detected. XRD peaks of the product, synthesized in a solution containing 5 g PEG6000 and 1.5 ml HCOOH for 5 h, are in accordance with those of the simulation and database. The (110) peak shows the preferential growth, corresponding to the hexaplates. characterized using SEM, TEM and HRTEM. CuS hexaplates with the (100) and (010) lattice planes at an angle of 120 degrees were detected oil the flat surface, and the (002) lattice plane on the edge. UV-vis absorption edge was detected at 610 nm (2.03 eV), and the PL emission at 361 nm (3.43 eV). Phase and morphology formations were also explained according to the experimental results. (C) 2009 Elsevier B.V. All rights reserved. Key Words: HYDROTHERMAL SYNTHESIS; OPTICAL-PROPERTIES; ROUTE MATERIALS LETTERS Volume: 64 Issue: 2 Pages: 111-114. DOI: 10.1016/j.matlet.2009.10.004 Published: JAN 31 2010. 319 L-Asparaginase Production by Bipolaris sp BR438 Isolated from Brown Rice in Thailand Lapmak Kodchakorn1, Lumyong Saisamorn1, Thongkuntha Sutheera1, Wongputtisin Pairote2 and Sardsud Uraporn1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Biology, Faculty of Science, Maejo University, Chiang Mai 50210, Thailand. 2 Abstract Thirty-six filamentous fungal isolates obtained from brown rice in Thailand were screened for their ability to produce L-asparaginase. Using modified Czapek Dox (mCD) agar containing L-asparagine and phenol red as indicator, 24 L-asparaginase producing fungal isolates could be preliminary identified by observing pink colour formation. It was found that isolate BR438 exhibited highest activity (6.3 +/- 0.65 U/ml) when cultured in the mCD medium containing 1% L-asparagine and 0.4% glucose at 30 degrees C for 72 h. Its asparaginase was also proved to be non-cytotoxic when tested against Vero cell lines. The identification based on internal transcribed spacer (ITS) regions of ribosomal DNA and morphological study showed that isolate BR438 was Bipolaris sp. and was closely related to Bipolaris australiensis and B. ovariicola. Key Words: ACUTE LYMPHOBLASTIC-LEUKEMIA; SOLID-STATE FERMENTATION; FUNGI; MICROORGANISMS; ACRYLAMIDE; THERAPY; AGENT CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 1 Pages: 159-163. Published: JAN 2010. 320 Luminescence and absorbance of highly crystalline CaMoO(4), SrMoO(4), CaWO(4) and SrWO(4) nanoparticles synthesized by co-precipitation method at room temperature Thongtem Titipun1, Kungwankunakorn Sukjit1, Kuntalue Budsabong2, Phuruangrat Anukorn3 and Thongtem Somchai3 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Electron Microscopy Research & Service Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Highly crystalline CaMoO, SrMoO(4), CaWO and SrWO(4) nanoparticles were successfully synthesized by the co-precipitation of mixtures of Ca(NO(3))(2)center dot 4H(2)O or Sr(NO(3))(2), and Na(2)MoO(4)center dot 2H(2)O or Na(2)WO(4) center dot 2H(2)O dissolved in ethylene glycol at room temperature (30 degrees C). Phases, morphologies, atomic vibrations and optical properties were analyzed by X-ray diffraction, transmission electron microscopy. Fourier transform infrared and Raman spectrophotometry, and ultraviolet-visible and photoluminescent spectroscopy. All products were proved to be MXO(4) (M = Ca and Sr, and X = Mo and W) with body-centered tetragonal scheelite structures, having round nanoparticles with the average sizes of 12.06 +/- 1.65, 16.40 +/- 2.44, 15.49 +/- 2.19, and 15.40 +/- 2.30 nm for CaMoO(4), SrMoO(4), CaWO(4) and SrWO(4), respectively. Their v(1)(A(g)), v(3)(B(g)), v(3)(E(g)), v(4)(B g), v(2)(Ag) and v(f,r,)(A(g)) vibration modes were also detected - being shifted to lower wavenumbers from MMoO(4) to MWO(4), due to the change of efficient atomic mass of the oscillating ions between X(6+) and O(2-) in the [XO(4)](2-) complexes. Band gaps of CaMoO(4), SrMoO(4), CaWO(4) and SrWO(4) were determined to be 5.07, 3.72, 5.40, and 4.47 eV, respectively. Photoluminescent (PL) emissions were at 414, 413, 418, and 414 nm for CaMoO(4), SrMoO(4), CaWO(4) and SrWO(4), respectively. 0 2010 Elsevier BM. All rights reserved. Key words: Nanostructured materials; Co-precipitation; Luminescence; Optical spectroscopy JOURNAL OF ALLOYS AND COMPOUNDS Volume: 506 Issue: 1 Pages: 475-481. DOI: 10.1016/j.jallcom.2010.07.033. Published: SEP 10 2010. 321 Mesoscale Simulation of a Very Heavy Rainfall Event over Mumbai, Using the Weather Research and Forecasting (WRF) Model Kirtsaeng Sukrit1,2,3, Chantara Somporn2,3 and Kreasuwun Jiemjai2,3 1 Thai Meteorological Department, Bangkok 10260, Thailand. Environmental Science Program, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Faculty of Science, Center for Environm Health Toxicology & Management of Chemical, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract The simulation of a severe weather phenomenon, in this case the unprecedented heavy rainfall over Mumbai in India on July 26, 2005, was selected for this study. The mesoscale numerical weather prediction model used here utilized the Advanced Research Weather Research Forecast model (version 3.0.1), as developed at the National Center for Atmospheric Research (NCAR) in the USA. The study used the Kian-Fritsch (KF), Betts-Miller-Janjic (BMJ) and Grell-Devenyi ensemble (GD) cumulus parameterization schemes across three nested domain configurations. The precipitation simulation results were compared with rainfall observation data from the Tropical Rainfall Measuring Mission. The NCEP analyses, with a 1 x 1 degree resolution and 26 levels, were utilized to verify the simulation’s resulting large-scale circulation pattern, moisture content and relative humidity fields. The 24-hour simulated cumulative rainfall data was created from the different measurements taken at 0300 UTC on July 27, 2005 over Mumbai. It can be observed that the maximum rainfall simulated from the KF, BMJ and GD schemes at 0000 UTC on July 25, 2005, under the initial conditions, was 48, 64 and 32 cm respectively, while the TRMM shows a maximum rainfall of 32 cm at that time. The centre of maximum rainfall was reduced drastically for all 0000 UTC measurements taken on July 26 (the following day), where the initial condition experiments simulated a rainfall amount of only 16 cm. The specific location of the intense rainfall around Mumbai was very-well simulated in the BMJ for 0000 UTC July 25 initial conditions. Key words: Heavy rainfall; Cumulus parameterization; WRF model CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 3 Pages: 429-442. Published: SEP 2010. 322 Microstructural, physical, and thermal analyses of Portland cement-fly ash-calcium hydroxide blended pastes Nochaiya T.1, Wongkeo W.1, Pimraksa K.2 and Chaipanich A.1 1 Department of Physics & Materials Science, Cement & Concrete Research Laboratory, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Industrial Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The effect of calcium hydroxide (CH) on the properties of Portland-fly ash cement pastes, at up to high-volume fly ash mixes has been investigated using normal consistency, setting time, compressive strength, thermal analysis and scanning electron microscope. CH as an additive material (5 and 10 wt%), lignite fly ash (FA) up to 50 wt% was used to produce Portland cement (PC)-FA-CH pastes at w/PC + FA ratio of 0.5. Water requirement for normal consistency was found to increase with increasing CH content while a decrease in initial setting time was found. Furthermore, the compressive strengths of all FA mixes with CH were found to be higher than the mixes without CH. Thermal analysis and scanning electron microscope were used to study the hydration of PC-FA-CH system. The results showed that the first phase transition detected by thermal analyses was attributed to ettringite, calcium silicate hydrate, gehlenite hydrate and was found to be higher in PC-FA-CH mixes than in pure Portland-FA cement paste resulting in an increase in compressive strength. Moreover, the hydration phases were also found to increase with increasing curing time. Overall, the results show that the additional of 5 wt% CH in Portland-FA mixes especially at high-volume FA mixes was found to accelerate FA pozzolanic reaction at early ages (7 and 28 days), resulting to an increase in compressive strength. Key Words: CONCRETE; STRENGTH; REACTIVITY; ACTIVATION JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY Volume: 100 Issue: 1 Pages: 101-108. DOI: 10.1007/s10973-009-0491-8. Published: APR 2010. 323 Microwave-assisted hydrothermal synthesis of Bi(2)S(3) nanorods in flower-shaped bundles Thongtem Titipun1, Pilapong Chalermchai1, Kavinchan Jutarat1, Phuruangrat Anukorn2 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Bi(2)S(3) nanorods in flower-shaped bundles were successfully synthesized from the decomposition of Bi-thiourea complexes under the microwave-assisted hydrothermal process. X-ray powder diffraction (XRD) patterns and field emission scanning electron microscopy (FE-SEM) show that Bi(2)S(3) has the orthorhombic phase and appears as nanorods in flower-shaped bundles. A transmission electron microscopic (TEM) study reveals the independent single Bi(2)S(3) nanorods with their growth along the [0 0 1] direction. A possible formation mechanism of Bi(2) S(3) nanorods in flower-shaped bundles is also proposed and discussed. Their UVvis spectrum shows the absorbance at 596 nm, with its direct energy band gap of 1.82 eV. (C) 2010 Elsevier B.V. All rights reserved. Key words: Semiconductors; Chemical synthesis; Luminescence; Scanning electron microscopy; Transmission electron microscopy; X-ray diffraction JOURNAL OF ALLOYS AND COMPOUNDS Volume: 500 Issue: 2 Pages: 195-199. DOI: 10.1016/j.jallcom.2010.03.240 Published: JUN 25 2010. 324 Microwave-assisted Pt-Co-Cr/C ternary compound preparation applied as a cathode catalyst for PEMFC Sarakonsri T.1, Thungprasert S.1, Klysubun W.2 and Vilaithong T.3 1 Center of Excellence for Innovation in Chemistry PERCH CIC, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima 30000, Thailand. 3 Department of Physics, Plasma & Beam Physics Research Facility, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The present study describes the preparation of catalyst nanocomposites comprised of 20 wt.% Pt-Co-Cr (2 : 1 : 1) particles attached on the surface of carbon Vulcan XC-72R by microwave radiation; cases of carbon being chemically treated and untreated are considered. Ethylene glycol was used as the solvent and electron source for the microwave-assisted reduction reaction, whereas H(2)PtCl(6) center dot xH(2)O, Co(NO(3))(3) center dot 6H(2)O and Cr(NO(3))(3) center dot 9H(2)O were used as metal precursors. The C powder surface was chemically modified by stirring the C in 8N H(2)O(2) for 48 h. For the nanocomposite in which C was not treated, EDS analysis showed a content of 4.9 wt.% Pt and 1.2 wt.% Cr with only a trace amount of Co. Higher Pt and Cr contents were observed in the catalyst sample prepared from treated carbon (5.6% Pt and 2.2% Cr), but no Co was detected. Chromium appeared as Cr(3)O(4) in both samples confirmed by the XAS spectrum. The obtained phase was therefore Pt-Cr(3)O(4)/C for both samples. The TEM results indicated that the average particle size of Pt-Cr(3)O(4) was 2.22+/-0.41 nm on treated C and 1.93+/-0.34 nm on untreated C. By the CV technique, it was observed that the catalytic activity of the treated carbon Pt-Cr(3)O(4) catalyst was not only higher than that of the untreated carbon Pt-Cr(3)O(4) catalyst, but also higher than that of the standard platinum catalyst. Key Words: OXYGEN REDUCTION; CARBON NANOTUBES; ELECTROCATALYSTS PHYSICA SCRIPTA Volume: T139 Article Number: 014007. DOI: 10.1088/0031-8949/2010/ T139/014007. Published: MAY 2010. 325 Microwave-assisted synthesis and characterization of SrMoO(4) and SrWO(4) nanocrystals Thongtem Titipun1, Phuruangrat Anukorn2 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Abstract SrMoO(4) and SrWO(4) nanocrystals have been synthesized using Sr(NO(3)) (2) and Na(2)MeO(4) (Me = Mo and W) in ethylene glycol by a microwave irradiation method at 50% of 180 W for 20 min. The product phases were detected using X-ray and selected area electron diffractions. They show the body-centered primitive tetragonal structure with the lattice parameters of a = b = 5.4007 and c = 12.0330 for SrMoO(4), and a = b = 5.4274 and c = 11.9241 for SrWO(4). X-ray diffraction patterns of the products, obtained by simulation using CaRIne version 3.1 program, are in accordance with those of the corresponding experiment and International Centre for Diffraction Data. A transmission electron microscope revealed the presence of nanocrystals composing the products, with their sizes in the ranges of 14-40 and 14-38 nm for SrMoO(4) and SrWO(4), respectively. Six different vibrations of nu (1)(A (g)), nu (3)(B (g)), nu (3)(E (g)), nu (4)(B (g)), nu (2)(A (g)), and nu (f.r.)(A (g)) were detected using a Raman spectrometer, and the nu (3)(F (2)) antisymmetric stretching and nu (4)(F (2)) bending modes of [MeO(4)](2-) tetrahedrons using a Fourier transform infrared spectrometer. Photoluminescence emissions of SrMoO(4) and SrWO(4), caused by the (1) T (2) -> (1) A (1) transition of electrons, were detected at 410 and 418 nm, respectively Key words: SrMoO(4); SrWO(4); Scheelite structure; Microwave irradiation; Luminescence JOURNAL OF NANOPARTICLE RESEARCH Volume: 12 Issue: 6 Pages: 2287-2294. DOI: 10.1007/s11051-009-9797-5. Published: AUG 2010. 326 Molecular dynamics simulations of Krytox-Silica-Nafion composite for high temperature fuel cell electrolyte membranes Yana Janchai1,2,3, Nimmanpipug Piyarat1,2,3, Chirachanchai Suwabun4, Gosalawit Rapee4, Dokmaisrijan Supaporn5, Vannarat Sornthep6, Vilaithong Thiraphat3,7 and Lee Vannajan Sanghiran1,2,3 1 Department of Chemistry, Faculty of Science, CSML, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center of Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 4 Petroleum & Petrochem College, Chulalongkorn University, Bangkok 10330, Thailand. 5 Division of Chemistry, School of Science, Walailak University, Nakon Si Thammarat 80161, Thailand. 6 National Electronics & Computer Technology Center, Large Scale Simulat Research Laboratory, Klongluang 12120, Pathumthani, Thailand. 7 Department of Physics, Fast Neutron Research Facility, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A five percent by weight of carboxylic acid terminated perfluoropolyether hybrid with silica (Krytox-Silica) in Nation composite polymer was used in the modification of a polymer electrolyte fuel cell membrane in order to improve its efficiency at high operating temperatures. Molecular dynamics (MD) simulations were carried out in order to understand the microscopic properties of two systems, Krytox-Silica in Nafion and pure Nafion. A model of five percent Krytox-Silica in a Nation composite polymer consisting of 15 Nafion side chains, 15 hydronium ions and one of Krytox-Silica was used. In another system, pure Nation was modeled without Krytox-Silica. Models with various amounts of water molecules and temperatures were simulated to study the water content and temperature effects. The results were in good agreement with the experiments and could be used to describe the application of Krytox-Silica Nafion composite at high temperatures. The effect of the amount of water molecules on the diffusion coefficient or proton 327 conductivity showed more deviations between 5% wt of Krytox-Silica Nafion composite and pure Nation system at lower water content (or higher temperature) than at high water content (or low temperature). According to the diffusion coefficient results, the percentage of water molecules at each temperature corresponded to the known experimental trend. Silica, as the water absorbent in the hybrid polymer membrane, did not have a strong interaction with water molecules or H(3)O(+) ions; thus the proton conductivities will not be highly affected by adding KrytoxSilica to the Nation. (C) 2010 Elsevier Ltd. All rights reserved. Key words: Molecular dynamic simulations; Krytox-Silica nafion composite; High temperature fuel cell membranes POLYMER Volume: 51 Issue: 20 Pages: 4632-4638. DOI: 10.1016/j.polymer.2010.07.036 Published: SEP 17 2010. 328 Molecular Markers for Identification of Stellantchasmus falcatus and a Phylogenic Study using the HAT-RAPD Method Wongsawad Chalobol and Wongsawad Pheravut Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Stellantchasmus falcatus is a minute intestinal fluke in the family Heterophyidae. Metacercariae, the infective stage, were reported in a marine fish, mullet Liza subviridis, and a fresh water fish, Dermogenus pusillus, in Thailand. Adults were found in chicks, rats, cats, and humans. Morphological studies were done for comparing Stellantchasmus sp. worms found in 2 different fish hosts; their shapes and organ arrangements were very similar except for the prepharynx length. Therefore, the present study aimed to compare their DNA fingerprints using the HAT-RAPD method for both types of Stellantchasmus and several other related species. Ten arbitrarily selected primers (OPA-04, OPA-09, OPN-02, OPN-03, OPN-09, OPN-12, OPP-11, OPR-15, OPX-13, and OPAD-01) were used. It was found that OPA-09, OPN-03, and OPAD-01 were able to generate S. falcatus specific fragments in mullets which consisted of 200, 760, and 280 bp, respectively. In addition, the results of morphologic, DNA fingerprinting, and phylogenetic analyses strongly suggest that the fresh water and marine specimens of Stellantchamus may be different species. Key Words: POLYMERASE-CHAIN-REACTION; AMPLIFIED POLYMORPHIC DNA; SCHISTOSOMA-MANSONI; OPISTHORCHIS-VIVERRINI; REACTION ASSAY; MULTIPLEX-PCR; COPRODIAGNOSIS; HAEMATOBIUM; SNAILS; WATER KOREAN JOURNAL OF PARASITOLOGY Volume: 48 Issue: 4 Pages: 303-307. DOI: 10.3347/kjp.2010.48.4.303. Published: DEC 2010. 329 Molecular Modeling of Peroxidase and Polyphenol Oxidase: Substrate Specificity and Active Site Comparison Nokthai Prontipa2, Lee Vannajan Sanghiran1,3,4,5 and Shank Lalida3,4,6 1 Faculty of Science, CSML, Chiang Mai University, Chiang Mai 50200, Thailand. Faculty of Science, Bioinformatics Research Laboratory (BiRL), Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Faculty of Science, Center of Innovation in Chemistry, Chiang Mai University, Chiang Mai 50200, Thailand. 5 Minister Education, Thailand Center of Excellence in Physics ThEP, Bangkok 10400, Thailand. 6 Faculty of Science, Phytochem Research Unit, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract Peroxidases (POD) and polyphenol oxidase (PPO) are enzymes that are well known to be involved in the enzymatic browning reaction of fruits and vegetables with different catalytic mechanisms. Both enzymes have some common substrates, but each also has its specific substrates. In our computational study, the amino acid sequence of grape peroxidase (ABX) was used for the construction of models employing homology modeling method based on the X-ray structure of cytosolic ascorbate peroxidase from pea (PDB ID:1APX), whereas the model of grape polyphenol oxidase was obtained directly from the available X-ray structure (PDB ID:2P3X). Molecular docking of common substrates of these two enzymes was subsequently studied. It was found that epicatechin and catechin exhibited high affinity with both enzymes, even though POD and PPO have different binding pockets regarding the size and the key amino acids involved in binding. Predicted binding modes of substrates with both enzymes were also compared. The calculated clocking interaction energy of trihydroxybenzoic acid related compounds show high affinity, suggesting specificity and potential use as common inhibitor to grape ascorbate peroxidase and polyphenol oxidase. Key words: peroxidase; polyphenol oxidase; browning reaction; molecular docking INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. Volume: 11 Issue: 9. Pages: 3266-3276. DOI: 10.3390/ijms11093266. Published: SEP 2010. 330 Molecular simulations of ultra-low-energy nitrogen ion bombardment of A-DNA in vacuum Ngaojampa Chanisorn1,2, Nimmanpipug Piyarat1,2, Yu Liangdeng3,4, Anuntalabhochai Somboon5 and Lee Vannajan Sanghiran1,2 1 Department of Chemistry, CSML, Chiang Mai University, Chiang Mai 50200, Thailand. Center of Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Physics & Materials Science, Plasma & Beam Physics Research Facility (PBP), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Commission Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 5 Department of Biology, Faculty of Science, Molecular Biology Laboratory, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract For investigating mechanisms involved in low-energy ion beam induced mutation, besides experiments using low-energy and low-fluence ions to bombard naked DNA, molecular simulations were carried out as an effort towards the insight in molecular interactions between ions and DNA. In the current study, Monte Carlo (MC) and molecular dynamics (MD) simulations were applied. The results of MC simulations provide some clues about the interaction energies and sites of preference of N-ion bombardment on an A-DNA short duplex strand. MD Simulations of a single N-ion moving towards the same DNA strand with different linear velocities corresponding to bombardment energies of 0.1, 1, 10 and 100 eV revealed information about changes in bond lengths and visibly distorted structures of bombarded nucleotides. The simulations demonstrated that ion-bombardment-induced DNA change in structure was not a random but preferential effect. (C) 2009 Elsevier Inc. All rights reserved. Key Words: STRAND BREAKS; FORCE-FIELD; GAS-PHASE; PLASMID; DAMAGE; IRRADIATION; DYNAMICS; ELECTRON; SPECTRUM; MODEL JOURNAL OF MOLECULAR GRAPHICS & MODELLING. Volume: 28 Issue: 6. Pages: 533-539. DOI: 10.1016/j.jmgm.2009.11.009. Published: FEB 26 2010. 331 Monitoring of arsenic in aquatic plants, water, and sediment of wastewater treatment ponds at the Mae Moh Lignite power plant, Thailand Nateewattana Jomjun1,4, Trichaiyaporn Siripen2, Saouy Maliwan3, Nateewattana Jintapat, Thavornyutikarn Prasak5, Pengchai Petch6 and Choonluchanon Somporn3 1 Gradute School, Inter-department of Environmental Management International, Chulalongkorn University, Bangkok 10330, Thailand. 2 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Soil Science & Conservation, Faculty of Agricuture, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Toxicol Program, Mahidol University, Bangkok 10700, Thailand. 5 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 6 Faculty of Engineering, Lab of Environmental Engineering, Mahasarakham University, Maha Sarakham, Thailand. Abstract Mae Moh is a risky area for arsenic contamination caused by the effluent from biowetland ponds in Mae Moh lignite-fuelled power plant. The objective of this study was to investigate the arsenic concentrations of Mae Moh biowetland ponds and determine the main factors which are important for arsenic phytoremediation in the treatment system. The result revealed that arsenic concentrations in the supernant were in the range of less than 1.0 mu g As L (-aEuro parts per thousand 1) to 2.0 mu g As L (-aEuro parts per thousand 1) while those in the sediment were in the range of 25-200 mu g As kg soil (-aEuro parts per thousand 1). Both values were below the Thailand national standard of 0.25 mg As L (-aEuro parts per thousand 1) for water and 27 mg As kg soil (-aEuro parts per thousand 1) for the soil. Arsenic accumulation in the biomass of 5 aquatic plants at the biowetland ponds ranged from 123.83 to 280.53 mg As kgPlant (-aEuro parts per thousand 1). Regarding the result of regression analysis (R (2) = 0.474 to 0.954), high concentrations of organic matter and other soluble ions as well as 332 high pH value in the sediment could significantly enhance the removal of soluble arsenic in the wetland ponds. From the regression equation of accumulated arsenic concentration in each aquatic plant, Eichhornia crassipes (Mart.) Solms. (R (2) = 0.954), Ipomoea aquatica Forsk. (R (2) = 0.850), and Typha angustifolia (L.) (R (2) = 0.841) were found to be preferable arsenic removers for wastewater treatment pond in the condition of low Eh value and high content of solid phase EC and phosphorus. On the other hand, Canna glauca (L.) (R (2) = 0.749) appeared to be favorable arsenic accumulator for the treatment pond in the condition of high Eh value and high concentration of soluble EC. Key words: Arsenic; Typha angustifolia (L.); Eichhornia crassipes (Mart.) Solms.; Canna glauca (L.); Ipomoea aquatica Forsk.; Colocasia esculenta (L.) Schott; Wetland pond; Mae Moh; Phytoremediation ENVIRONMENTAL MONITORING AND ASSESSMENT Volume: 165 Issue: 1-4. Pages: 585-594 DOI: 10.1007/s10661-009-0970-1. Published: JUN 2010. 333 Monte Carlo Investigation of Ferromagnetic Properties Under Compressive Stress Laosiritaworn Yongyut1,2, Ananta Supon1 and Yimnirun Rattikorn3 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Highter Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 3 Institute of Science, School Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract In this work, we used Monte Carlo simulations to model the three-dimensional ferromagnetic Ising spins under the influence of uniaxial and hydrostatic compressive mechanical stresses. The study was performed with a cluster-flip algorithm to investigate how the magnetic properties including critical behaviors depend on temperatures and the applied mechanical stresses. From the results, the magnetic profiles as a function of temperature were obtained and it was found that the applied stresses lowered the magnetic phase transition point. However, the critical exponents to the relevant observables did not significantly change. This suggests universality behavior in magnetic systems under loading environment. Key Words: 3-DIMENSIONAL ISING-MODEL; HIGH-PRESSURE; CRITICALBEHAVIOR; ULTRATHIN FILMS; TRANSITION; TEMPERATURE; LATTICE; FUTURE CHIANG MAI JOURNAL OF SCIENCE Volume: 37 Issue: 2. Pages: 252-259. Published: MAY 2010. 334 Muscodor cinnamomi, a new endophytic species from Cinnamomum bejolghota Suwannarach Nakarin1, Bussaban Boonsom1, Hyde Kevin D.2 and Lumyong Saisamorn1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Abstract Muscodor cinnamomi is described as a new species, endophytic within leaf tissues of Cinnamomum bejolghota (Lauraceae) in Doi Suthep-Pui National Park, Northern Thailand. Molecular analysis indicated differences from the five previously described Muscodor spp. Volatile organic compounds analysis showed that M. cinnamomi produced azulene (differentiating it from M. crispans) but did not produce naphthalene (differentiating it from M. albus, M. roseus, and M. vitigenus). Key words: sterile ascomycete; cinnamon; endophytes; volatile compounds MYCOTAXON Volume: 114 Pages: 15-23 DOI: 10.5248/114.15. Published: OCT-DEC 2010. 335 Nanostructured carbon nanotubes/copper phthalocyanine hybrid multilayers prepared using layer-by-layer self-assembly approach Baba Akira1,2, Kanetsuna Yoshinori1,2, Sriwichai Saengrawee1,2, Ohdaira Yasuo1,2, Shinbo Kazunari1,2, Kato Keizo1,2, Phanichphant Sukon3 and Kaneko Futao1,2 1 Center for Transdisciplinary Research, Nishi Ku, Niigata University, Niigata 9502181, Japan. Gradute School of Science & Technology, Nishi Ku, Niigata University, Niigata 9502181, Japan. 3 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract In this report, we demonstrate a convenient method of fabricating singlewalled carbon nanotubes/organic semiconductor hybrid ultrathin multilayers using a layer-by-layer self-assembly approach. Single-walled carbon nanotubes were solubilized by water-soluble cationic alcian blue pyridine variant and anionic copper phthalocyanine-3,4’,4 “,4” -tetrasulfonic acid tetrasodium salt, which were then utilized for electrostatic layer-by-layer multilayer fabrication. The solubilization ability of single-walled carbon nanotubes was studied in water by UV-vis absorption spectroscopy. The composites were highly dispersed owing to the pi-pi interactions. In situ surface plasmon resonance spectroscopy during the layer-by-layer multilayer fabrication indicated a stepwise increase in reflectivity, indicating the successive formation of nanostructured hybrid ultrathin films. Cyclic voltammetry revealed that the electroactivity of the hybrid film was enhanced by the incorporation of single-walled nanotubes. (C) 2009 Elsevier B.V. All rights reserved. Key Words: PLASMON RESONANCE SPECTROSCOPY; PHOTOINDUCED ELECTRONTRANSFER; DONOR-ACCEPTOR NANOHYBRIDS; ULTRATHIN POLYMER-FILMS; SINGLE-WALL; POLY(3,4-ETHYLENEDIOXYTHIOPHENE) FILMS; ASCORBIC-ACID; PORPHYRIN; SOLUBILIZATION THIN SOLID FILMS Volume: 518 Issue: 8 Pages: 2200-2205. DOI: 10.1016/j.tsf.2009.09.052 Published: FEB 1 2010. 336 Oblongolides from the Endophytic Fungus Phomopsis sp BCC 9789 Bunyapaiboonsri Taridaporn1, Yoiprommarat Seangaroon1, Srikitikulchai Prasert1, Srichomthong Kitlada1 and Lumyong Saisaimorn2 1 National Center for Genetic Engineering & Biotechnology (BIOTEC), Klongluang 12120, Pathumthani, Thailand. 2 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Six new oblongolides, W1, W2, X, Y, and Z (1-3, 6, 7) and 2-deoxy-4 alphahydroxyoblongolide X (4), and the known compounds oblongolide (8), oblongolides T, C, and Q (5, 9, 10), and (-)-5-methylmellein were isolated from the endophytic fungus Phomopsis sp. BCC 9789. Compound 7 showed anti-HSV-1 activity (IC(50) = 14 mu M) and cytotoxic activities against KB, BC, NCI-H187, and nonmalignant (Vero) cell lines with respective IC(50) values of 37, 26, 32, and 60 mu M. Cytotoxic activity against the BC cell line was also observed for compound 6, with all IC(50) value of 48 mu M. Key Words: METABOLIC PRODUCTS; ASSAY; OBLONGA; DIMERS JOURNAL OF NATURAL PRODUCTS Volume: 73 Issue: 1 Pages: 55-59. DOI: 10.1021/ np900650c. Published: JAN 2010. 337 Occurrence and diversity of basidiomycetous endophytes from the oil palm, Elaeis guineensis in Thailand Pinruan Umpava1,2, Rungjindamai Nattawut1, Choeyklin Rattaket1, Lumyong Saisamorn2, Hyde Kevin D.3 and Jones E. B. Gareth1 1 NSTDA, National Center for Genetic Engineering & Biotechnology, BIOTEC Bioresources Technology Unit, Khlong Luang 12120, Pathum Thani, Thailand. 2 Department of Microbiology, Faculty of Science, Chiang Mai University, Chiang Mai 50000, Thailand. 3 School for Science, Mae Fah Luang University, Chiang Rai, Thailand. Abstract Fungal endophytes associated with the oil palm, Elaeis guineensis, at an oil palm plantation in Trang Province, Thailand were investigated. Two samplings at the same site yielded 162 and 178 morphotypes. The overall colonization rate was 81 and 87%, respectively, with non-sporulating species being most common. Twenty strains were identified as basidiomycetes using morphological characteristics and confirmed at the molecular level using ribosomal DNA, LSU and ITS sequence data. Species included Fomitopsis cf. meliae, F. cf. ostreiformis, F. cf. pinicola, Perenniporia sp., Pycnoporus sanguineus, Trametes lactinea, and Schizophyllum commune. Leaves supported the greatest number of basidiomycetous taxa (11 strains) with five and four taxa from petioles and rachides, respectively. Twelve taxa produced poroid basidiomes in PDA culture, while the remainder had clampconnections. Basidiomycetes occurring as saprobes on oil palm were also recorded and compared with those present as endophytes. Key Words: FOLIAR FUNGAL ENDOPHYTES; MULTIPLE SEQUENCE ALIGNMENT; ATTACHED BEECH BRANCHES; MOLECULAR CHARACTERIZATION; TAXONOMIC PLACEMENT; MYCORRHIZAL FUNGI; FUCUS-SERRATUS; RDNA SEQUENCES; LEAVES; IDENTIFICATION FUNGAL DIVERSITY Volume: 41 Issue: 1 Pages: 71-88. DOI: 10.1007/s13225-010-0029-1 Published: MAR 2010. 338 Patterns of occurrence of myxomycetes on lianas Ko Thida Win Ko1,4, Stephenson Steven L.2, Hyde Kevin D.3, Rojas Carlos2 and Lumyong Saisamorn1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Biological Science, University of Arkansas, Fayetteville, AR 72701 USA. 3 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. 4 Mushroom Research Foundation, Chiang Mai 50150, Thailand. Abstract Patterns of occurrence of myxomycetes on different types of lianas were assessed at the Mushroom Research Centre in northern Thailand during the rainy season of Jun.-Sep. 2008. A total of 30 species representing, 15 genera of myxomycetes, were obtained from a series of moist chamber cultures prepared with samples of living and dead lianas. The results of the present study appear to indicate that such biodiversity parameters of myxomycete communities as species richness, species diversity, taxonomic diversity and species composition vary on lianas as a result of the differences that exist in height above the ground, bark texture and liana diameter. Three of the species (Physarum didermoides, Physarum aeneum and Perichaena dictyonema) recorded in the present study are new for Thailand. (C) 2009 Elsevier Ltd and The British Mycological Society. All rights reserved. Key Words: BIODIVERSITY ASSESSMENT; TROPICAL FORESTS; ECOLOGY; MICROHABITAT; LITTER; THAILAND; ECUADOR; REGIONS FUNGAL ECOLOGY Volume: 3 Issue: 4 Pages: 302-310. DOI: 10.1016/j.funeco.2009.11.005 Published: NOV 2010. 339 Pervaporation flow injection analysis for the determination of sulphite in food samples utilising potassium permanganaterhodamine B chemiluminescence detection Satienperakul Sakchai1, Phongdong Pornthana1 and Liawruangrath Saisunee2,3 1 Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand. Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Postgraduate Education & Research Program Chemistry PERCH, Faculty of Science, CIC, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract A simple pervaporation flow injection chemiluminescence (PFI-CL) procedure was utilised as an on-line separation for the analysis of contaminated sulphite in food samples. The method involves the injection of standard and/or sulphite sample solutions into a 0.20 M sulphuric acid donor stream. Sulphite is converted to sulphur dioxide and transported to the donor chamber of a pervaporation module. The sulphur dioxide gas then evaporates into the headspace and diffuses across a semi-permeable PTFE membrane into an acceptor stream containing 0.75% (m/v) sodium hexametaphosphate and 1.0 mg L(-1) rhodamine B in 0.02 M H(3)PO(4), which functions as a carrier solution for the chemiluminescence detection. The sulphur dioxide in the acceptor stream merges at a T-piece with a reagent stream consisting of potassium permanganate (8.0 x 10(-5) M) prepared in the acidic sodium hexametaphosphate carrier solution. The elicited chemiluminescence intensity of the resulting reaction mixture was measured at a red sensitive photomultiplier tube operated at a voltage of 1.00 kV. Optimal experimental conditions for an on-line determination of sulphite were investigated. The second-order polynomial calibration curve was developed over the concentration range of 0.5-10.0 mg L(-1) sulphite with a resulting equation of I = -0.239C(2) + 4.846C - 1.64, r(2) = 0.9997. The detection limit was found to be 0.2 mg L(-1) with a sampling frequency of 30 h(-1). The effects of common anionic and cationic interferences were also investigated. The proposed PFI procedure was successfully applied to the determination of sulphite in different food samples. The PFI data was validated versus standard differential pulse polarography. (C) 2010 Elsevier Ltd. All rights reserved. Key words: Sulphite; Flow injection; Pervaporation; Chemiluminescence; Acidic potassium permanganate; Rhodamine B FOOD CHEMISTRY Volume: 121 Issue: 3 Pages: 893-898. DOI: 10.1016/j.foodchem. 2010.01.013 Published: AUG 1 2010. 340 Phase development and dielectric responses in PMN-BNT ceramics Jaitanong N., Vittayakorn W. C. and Chaipanich A. Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract (1 - x)Pb(Mg(1/3)Nb(2/3))O(3)-x(Bi(0.5)Na(0.5))TiO(3) ceramics were prepared by the conventional mixed-oxide method. All compositions show complete perovskite solid solutions and the structure to change from cubic to rhombohedral at x = 0.5. The dielectric constant and dielectric loss tangent were measured as a function of both temperature and frequency. The results indicated a relaxor ferroelectric behavior for all ceramics. The temperature at maximum of the dielectric constant of PMN-BNT ceramics were seen to increase with increasing BNT content. Moreover, the broadest dielectric peak occurs at x = 0.9, which leads to a tnorphotropic phase boundary in this system. (C) 2010 Elsevier Ltd and Techna Group S.r.l. All rights reserved. Key Words: FREE PIEZOELECTRIC CERAMICS; LEAD-FREE CERAMICS; (1-X) BATIO3-XBI(0.5)NA(0.5)TIO(3) CERAMICS; FERROELECTRIC PROPERTIES; ELECTRICAL-PROPERTIES CERAMICS INTERNATIONAL Volume: 36 Issue: 4 Pages: 1479-1483 DOI: 10.1016/j. ceramint.2010.01.003 Published: MAY 2010. 341 Phase transition anomaly observed in PbZrO3/Al2O3 nanocomposites Puchmark C.2, Rujijanagul G.1 and Milne S. J.3 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand. 3 Institute for Research, University of Leeds, Leeds LS2 9JT, W Yorkshire, England. Abstract Effects of Al2O3 nanoparticle additions on the phase transitions of PbZrO3/ Al2O3 nanocomposites were investigated. The nanocomposites were prepared by a solid-state reaction method. The phase transition behaviour of the composites was studied by dielectric measurements and differential scanning calorimetry. Nanoparticle additions produced a significant reduction in the temperature of the phase transition from ferroelectric to antiferroelectric phase during the cooling measurement. This resulted in a large thermal hysteresis. The results indicated that the intermediate ferroelectric phase of PbZrO3 is very sensitive to the Al2O3 impurity. It is proposed that changes in defec Key Words: FERROELECTRIC PHASE; (PB0.90BA0.10)ZRO3 CERAMICS; LEAD ZIRCONATE PHASE TRANSITIONS Volume: 83 Issue: 10-11 Special Issue: SI Pages: 868-874. Article Number: PII 929908112 DOI:10.1080/01411594.2010.509163. Published: 2010. 342 Phase transition behavior in (1-x)PZT-xBiAlO3 ceramics Tawichai N.1, Tunkasiri T.1,2, Eitssayeam S.1, Pengpat K.1 and Rujijanagul G.1,3 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. 3 Science & Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In this study, a new ceramic with the composition (1 - x)Pb(Zr0.52Ti0.48)O3xBiAlO3 was fabricated using the solid state method. Phase transition characteristic and dielectric response were investigated. With increase in the content of BiAlO3, a transformation from the tetragonal to the rhombohedral phase was observed. The addition also enhanced the degree of phase transition diffuseness and produced a decrease in the ferroelectric-to-paraelectric phase transition temperature. The results suggested that BiAlO3 has a strong effect on the transition behavior of the solid solution. It is proposed that lattice distortion and compositional fluctuation are responsible for these effects. Key Words: DIELECTRIC-PROPERTIES; TITANATE; BIALO3 PHASE TRANSITIONS Volume: 83 Issue: 10-11 Special Issue: SI Pages: 994-1000. Article Number: PII 929905534 DOI:10.1080/01411594.2010.509612. Published: 2010. 343 Phenolic content and antioxidant properties of green chilli paste and its ingredients Ruanma Kamonra, Shank Lalida and Chairote Griangsak Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Green chilli paste and its ingredients (chilli, red onion and garlic) from different stages of processing were analysed for total phenolic content and antioxidant properties, i.e. total antioxidant capacity, DPPH radical scavenging activity, and beta-carotene bleaching activity. The effects of processing stage on total phenolic content and antioxidant properties of green chilli paste and its ingredients were discussed, along with the correlation between the total phenolic content and the antioxidant properties. Key Words: COOKING; ONION; L.; FLAVONOIDS; VEGETABLES; EXTRACTS; MATURITY MAEJO INTERNATIONAL JOURNAL OF SCIENCE AND TECHNOLOGY. Volume: 4 Issue: 2 Pages: 193-200. Published: MAY-AUG 2010. 344 Plasma immersion low-energy-ion bombardment of naked DNA Sarapirom S.1, Sangwijit K.2, Anuntalabhochai S.2 and Yu L. D.1,3 1 Department of Physics & Materials Science, Faculty of Science, Plasma & Beam Physics Research Facility, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Biology, Faculty of Science, Molecular Biology Laboratory, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Commission Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. Abstract Low-energy ion irradiation of DNA is of great interest in fundamental studies on mechanisms involved in low-energy ion beam induced mutation, plasma sterilization and ionizing radiation risk of lives. We have made the first attempt to use low-energy ions in plasma immersion ion implantation and deposition (PIII-D) to bombard naked plasmid DNA to investigate effect on the DNA structural modification and mutation. Naked DNA samples were immersed in either argon or nitrogen plasma in low pressure and then bombarded by ions in the plasma in different conditions, namely, using a low bias of -2.5 kV, or no bias, in which the sample holder was either grounded or not grounded, to low fluences of 10(11), 10(12) and 10(13) ions/cm(2). The plasma-treated DNA was transferred into bacteria E. coli. Mutation was found from the bacterial colonies when DNA was bombarded with the bias, but not found when DNA was bombarded without a bias. This indicates that ions with energy only at the order of the thermal energy cannot induce mutation but with low-energy of key the ions can. Subsequent gel electrophoresis and DNA sequencing analyzed the DNA structural changes and found certain modifications in the DNA forms. (C) 2010 Elsevier B.V. All rights reserved. Key words: Plasma immersion ion implantation and deposition (PIII-D); Low-energy ion; Naked DNA; Mutation SURFACE & COATINGS TECHNOLOGY. Volume: 204 Issue: 18-19. Pages: 2960-2965 DOI: 10.1016/j.surfcoat.2010.02.036 Published: JUN 25 2010. 345 Polyethyleneimine/sodium dodecyl sulphate adsorbed silica particles and their adsorption properties Janhom Sorapong1,2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center for Innovation Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Silica particles were modified in aqueous solution with polyethyleneimine (PEI) and sodium dodecyl sulphate (SOS), in order to introduce a hydrophobic surface on the silica particles. The properties of adsorbed layers have been explored using spectrophotometry and the factors affecting PEI adsorption on silica particles, such as material liquor ratios (MLR), PEI concentration, pH in the range of 3-12 and electrolyte concentration of 0-0.100 M NaCl. The PEI adsorption on silica particles at various pHs indicated that the adsorbed amount of PEI increased with pH. The influence of NaCl on PEI adsorption revealed that increasing NaCl concentration decreased the absorbed amount of PEI. The adsorption of SDS on PEI-modified silica particles was then investigated using nephelometry. The influence of NaCl and pH on SDS adsorption on the PEI-modified silica particles was also studied. The increasing NaCl concentration decreased the adsorbed amount of SDS. The SDS adsorption at various pH indicated that the adsorbed amount of SOS increased with the decrease in pH. In addition, the surface charge of the PEI-modified and PEI/SDS-modified silica particles were investigated via zeta potential measurements. The Langmuir adsorption isotherm of the soybean oil on the PEI-modified silica particles with corresponding was determined. The oil sorption amount on the PEI-modified and PEI-SDS-modified silica particles were increased in comparison to the unmodified silica surface with the highest adsorbed amounts of soybean oil of a 2 g(oil)/g(silica). The size and morphology of the resulting particles were examined by polarizing microscope and stereo microscope. (C) 2010 Elsevier B.V. All rights reserved. Key Words: SURFACES; PH; POLY(ETHYLENEIMINE); POLYETHYLENIMINE COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS Volume: 369 Issue: 1-3 Pages: 186-190 DOI: 10.1016/j.colsurfa.2010.08.025 Published: OCT 20 2010. 346 Polymer-assisted hydrothermal synthesis of Bi(2)S(3) nanostructured flowers Pilapong Chalermchai1, Thongtem Titipun1 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Nanostructured Bi(2)S(3) was hydrothermally produced from Bi(2)O(3) and thiocarbohydrazide in acidic solutions containing PVA, PEG and PVP. By using XRD. SAED and Raman spectrometry, the products were orthorhombic Bi(2)S(3), with four vibration modes at 139.6, 253.7, 310 and 968.9 cm(-1). The phase was also in accordance with the diffraction patterns obtained by simulation. SEM, TEM and HRTEM show that the products are clusters of nanorods produced in polymerfree solution, and nanostructured flowers of nanospears, nanorods and nanoplates in the respective PVA-, PEG- and PVP-added solutions, with their growths in the same direction of [0 0 1]. A formation mechanism was also proposed according to their phase and morphologies. (C) 2009 Elsevier Ltd. All rights reserved. Key Words: FACILE SYNTHESIS; NANORODS; MORPHOLOGIES JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS Volume: 71 Issue: 4. Special Issue: SI Pages: 712-715. DOI: 10.1016/j.jpcs.2009.12.073. Published: APR 2010. 347 Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach Chontananarth Thapana and Wongsawad Chalobol Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50202, Thailand. Abstract The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5’ -GGCCAACGCAATCGTCATCC-3’) and Hapt_R (5’ -GCGTCGGGTTTCAGACATGG-3’), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%. Key Words: OPISTHORCHIS-VIVERRINI; CHIANG-MAI; THAILAND; FISH; IDENTIFICATION; HETEROPHYIDAE; MORPHOLOGY; TREMATODES; INFECTION; CERCARIAE KOREAN JOURNAL OF PARASITOLOGY. Volume: 48 Issue: 4 Pages: 343-346. DOI: 10.3347/kjp.2010.48.4.343 Published: DEC 2010. 348 Processing and properties of nanoporous hydroxyapatite ceramics Raksujarit A.2, Pengpat K.1, Rujijanagul G.1 and Tunkasiri T.1 1 Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Science, Faculty of Science & Technology, Chiang Mai Rajabhat University, Chiang Mai 50300, Thailand. 2 Abstract Method for fabrication and properties of nanoporous hydroxyapatite (HA) ceramic were described in the present work. The nanoporous hydroxyapatite was derived from nano hydroxyapatite powder and polyvinyl alcohol (as a pore former). The HA nanopowder was obtained from vibro-milling for 4 h. The nanoporous ceramics were sintered at 1200 degrees C. Properties of the nanoporous ceramics were investigated using various methods. Average porosity of the final product was found to be 64.6 +/- 1.4%. Open and interconnected pores were obtained with an average pore size less than 100 nm, confirming the nanoporous structure of this ceramic. A high bending strength of 14.7 +/- 3.2 MPa for the nanoporous ceramic, shows significant promise as a potential bone repairing material. (C) 2009 Elsevier Ltd. All rights reserved. Key Words: SCAFFOLD; APATITE; COMPOSITES; BONE MATERIALS & DESIGN. Volume: 31 Issue: 4 Pages: 1658-1660. DOI: 10.1016/j.matdes.2009.06.050. Published: APR 2010. 349 Production of Monoclonal Antibody to Acaricide Dicofol and Its Derivatives Hongsibsong Surat4, Prapamontol Tippawan5, Suphavilai Chaisuree3, Wipasa Jiraprapa3, Pattarawarapan Mookda2 and Kasinrerk Watchara1 1 Faculty of Associated Medical Science, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Research Institute for Health Science (RIHES), Center Molecular & Cell Biology Infectious Diseases, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Gradute School, Health Science Institute Program, Chiang Mai University, Chiang Mai 50200, Thailand. 5 Research Institute for Health Science (RIHES), Center for Pollution & Environmental Health Research, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract In Thailand detection of acaricide dicofol residues has been sporadically performed due to the limitation of analytical techniques. Conventional analytical methods for detecting dicofol residues most often use chromatographic-based techniques. Our ultimate aim is to develop an alternative method for rapidly analyzing dicofol residues in vegetables and fruit samples. Here we report the production of monoclonal antibodies specific to dicofol and its derivatives. Hapten-protein carriers were prepared by linking succinic anhydride to dichlorobenzhydrol (DCBH), which was then conjugated to bovine serum albumin (BSA) and oval albumin (OVA). DCBH-BSA conjugate was used as immunogen while DCBH-OVA conjugate was used as capture antigen for competitive inhibition assay. Female BALB/c mice were immunized with DCBH-BSA conjugate subcutaneously, and antibody (Ab) level was determined 2 weeks after the last immunization. Spleen cells producing high titer antibody were isolated and fused with myeloma cells of P3.X6.Ag8.653. After limiting dilutions, antibody produced by one clone had high affinity, which was found to be of IgG1 with kappa light chain. Specificity and inhibition concentrations of the monoclonal antibody (MAb) were determined by competitive indirect ELISA with dicofol, and its 50% (IC(50)) was 0.28 mu g/mL. Working ranges of the developed immunoassay were from 0.07 to 25 mu g/mL. Hence, the prepared MAb will be able to be applied for immunoassay development for detecting dicifol residue in vegetables and fruits far below the maximum residue limit such that 5 g of fruits and berries can be detected below 0.1 mg/kg. Key Words: HAPTEN SYNTHESIS; IMMUNOASSAY; DDT; PESTICIDES; INSECTICIDES; RESIDUES HYBRIDOMA. Volume: 29 Issue: 6 Pages: 495-500. DOI: 10.1089/hyb.2010.0051. Published: DEC 2010. 350 Quantitative analysis of a complex metal carbide formed during furnace cooling of cast duplex stainless steel using EELS and EDS in the TEM Kuimalee Surasak2, Chairuangsri Torranin1, Pearce John T. H.3, Edmonds David V.4, Brown Andrew P.4 and Brydson Rik M. D.4 1 Department of Industrial Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 National Metals & Materials Technology Center, Pathum Thani 12120, Thailand. 4 Institute for Materials Research, School Process Environmental & Materials Engineering, University Leeds, Leeds LS2 9JT, W Yorkshire, England. Abstract In this work, a method to determine the atomic ratio of Mo and C within complex metal carbides using EELS in the TEM has been developed. The method is based on the determination of k-factors for given experimental conditions from the EEL spectra of Mo(2)C and MoO(3) standards, which had been independently checked by XRD and EPMA. Factors affecting the k(Mo/C) value of the Mo(2) C standard were also investigated and the value was shown to be insensitive to background subtraction window width but sensitive to prolonged irradiation and specimen thicknesses above a critical value. The method and k-factor obtained from the Mo(2)C standard was applied to spectra from a complex metal carbide precipitate formed during furnace cooling of a cast duplex stainless steel. Using EELS and EDS in the TEM, the composition was estimated to be (Cr(1.52)Fe(2.33) Mo(1.25)Ni(0.17)Si(0.46))C, which is close to M(6)C stoichiometry, and the structure was confirmed by electron diffraction. (C) 2010 Elsevier Ltd. All rights reserved. Key words: Duplex stainless steel; Microstructure; Carbide; Quantitative analysis; Transmission electron microscopy (TEM); Electron energy loss spectroscopy (EELS) MICRON. Volume: 41 Issue: 5 Pages: 423-429. DOI: 10.1016/j.micron.2010.02.009 Published: JUL 2010. 351 Randomized controlled trial of live lactobacillus acidophilus plus bifidobacterium bifidum in prophylaxis of diarrhea during radiotherapy in cervical cancer patients Chitapanarux Imjai1, Chitapanarux Taned2, Traisathit Patrinee3, Kudumpee Sudkaneung1, Tharavichitkul Ekkasit1 and Lorvidhaya Vicharn1 1 Department of Radiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Statistics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Background: Radiation-induced diarrhea is frequently observed during pelvic radiotherapy. This study was performed to determine the ability of a probiotic containing live lactobacillus acidophilus plus bifidobacterium bifidum to reduce the incidence of radiation-induced diarrhea in locally advanced cervical cancer patients. Methods: Patients who were undergoing pelvic radiotherapy concurrent with weekly cisplatin were randomly assigned to a study drug or placebo, in a double-blind study. Diarrhea was graded weekly according the Common Toxicity Criteria (CTC) system. Stool consistency and white and red blood cell count in stool were also assessed. The primary endpoint was to reduce the incidence of diarrhea, defined by a CTC grade 2 or more, and the need for anti-diarrheal medication. Results: A total of 63 patients were enrolled. Grade 2-3 diarrhea was observed in 45% of the placebo group (n = 31) and 9% of the study drug group (n = 32) (p = 0.002). Anti-diarrheal medication use was significantly reduced in the placebo group (p = 0.03). The patients in the study drug group had a significantly improved stool consistency (p < 0.001). Conclusions: Live lactobacillus acidophilus plus bifidobacterium bifidum reduced the incidence of radiation-induced diarrhea and the need for anti-diarrheal medication and had a significant benefits on stool consistency. Key Words: ANTIBIOTIC-ASSOCIATED DIARRHEA; FERMENTED MILK; PELVIC RADIOTHERAPY; RADIATION ENTERITIS; DOUBLE-BLIND; PREVENTION; MANAGEMENT; SYMPTOMS; CULTURES; CHILDREN RADIATION ONCOLOGY. Volume: 5 Article Number: 31 DOI: 10.1186/1748-717X-5-31. Published: MAY 5 2010. 352 Remarks on Asymptotic Centers and Fixed Points Kaewkhao A.1 and Sokhuma K.2 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mathematics, Faculty of Science, Burapha University, Chon Buri 20131, Thailand. Abstract We introduce a class of nonlinear continuous mappings defined on a bounded closed convex subset of a Banach space X. We characterize the Banach spaces in which every asymptotic center of each bounded sequence in any weakly compact convex subset is compact as those spaces having the weak fixed point property for this type of mappings. Key Words: NONEXPANSIVE-MAPPINGS; PROPERTY ABSTRACT AND APPLIED ANALYSIS. Article Number: 247402 DOI: 10.1155/2010/247402 Published: 2010. 353 Reverse flow injection spectrophotometric for determination of aluminium (III) Norfun Poachanee1,2, Pojanakaroon Teraboon1,2 and Liawraungrath Saisunee1 1 Department of Chemistry, Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Institute of Science & Technology, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A reverse flow injection analysis (rFIA) spectrophotometric method has been developed for the determination of aluminium(III). The method was based on the reaction of Al(III), quercetin and cetyltrimethylammonium bromide (CTAB), yielding a yellow colored complex in an acetate buffer medium (pH 5.5) with absorption maximum at 428 nm. The rFIA parameters that influence the FIA peak height have been optimized in order to obtain the best sensitivity and minimum reagent consumption. A linear relationship between the relative peak height and Al(III) concentrations were obtained over the concentration range of 0.02-0.50 mg L(-1) with a correlation coefficient of 0.9998. The limit of detection (LOD, defined as 3 sigma) and limit of quantification (LOQ defined as 10 sigma) were 0.007 and 0.024 mg L(-1), respectively. The repeatability was 1.10% (n = 11) for 0.2 mg L(-1) Al(III). The proposed method was applied to the determination of Al(III) in tap water samples with a sampling rate of 60 h(-1). Results obtained were in good agreement with those obtained by the official ICP-MS method at the 95% confidence level. (C) 2010 Elsevier By. All rights reserved. Key words: Reverse flow injection; Aluminium; Quercetin; Tap waters TALANTA. Volume: 82 Issue: 1 Pages: 202-207. DOI: 10.1016/j.talanta.2010.04.019. Published: JUN 30 2010. 354 SCHUADER’S CONJECTURE ON CONVEX METRIC SPACES Butsan T., Dhompongsa S. and Fupinwong W. Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract We first prove that the Schauder’s conjecture holds for convex metric spaces, thus compact convex subsets of a CAT(0) space have the fixed point property for continuous mappings. We then obtain a continuous selection of a lower semicontinuous mapping with compact convex values defined on a compact convex subset of a convex metric space. Consequently, the Kakutani fixed point theorem is extended to a convex metric space. Key Words: FIXED-POINT PROPERTY; SCHAUDERS CONJECTURE; COMPACT; SETS JOURNAL OF NONLINEAR AND CONVEX ANALYSIS. Volume: 11 Issue: 3. Special Issue: SI Pages: 527-535. Published: 2010. 355 Selection of Starter Cultures for the Production of Vegetarian Kapi, a Thai Fermented Condiment Wittanalai Suttida1, Chandet Nopakarn2 and Rakariyatham Nuansri2 1 Division Biotechnology, Graduate of School, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Kapi is a traditional fermented shrimp paste that serves as a flavoring in various Thai foods. 7 kapi samples from local markets in Chiang Mai, Trad, Rayong, Chon Buri and Nakhon Sawan, Thailand, were used as a source for the isolation of their bacterial strains. The total viable count of the 7 samples was in the range of 8.48 - 9.30 log cfu/g. A total of 23 isolates were obtained, 10 of which had the ability to produce both proteolytic and amylase activity and these were selected to produce vegetarian kapi using mung bean protein as a substrate. Among the 10 strains, isolate RY1 which was identified as Bacillus subtilis by 16s rDNA analysis, was selected to be the starter culture due to its ability to produce the highest total quantities of nitrogen (110.07 +/- 2.76 g/kg), free amino acids (7.92 +/- 1.34 mg/g) as well for its low ammoniacal nitrogen content (5.63 +/- 0.20 g/kg). The quality of the vegetarian kapi produced by isolate B. subtilis RY1 was compared to that of the commercial vegetarian kapi samples. There were significant differences (p<0.05) in moisture, protein, pH, free amino acid and ammoniacal nitrogen between the vegetarian kapi produced from B. subtilis RY1 and the commercial vegetarian kapi. In addition, organoleptic evaluation scores showed no significant differences between laboratory vegetarian kapi and commercial vegetarian kapi. With the use of the starter culture B. subtilis RY1, the vegetarian kapi could be produced in a shorter period compared with the commercial vegetarian kapi. Key Words: LACTIC-ACID BACTERIA; SHRIMP PASTE; BACILLUS-SUBTILIS; TEMPEH FERMENTATION; SP-NOV.; PROFILE; GROWTH; NATTO; SOUP; FISH CHIANG MAI JOURNAL OF SCIENCE. Volume: 37. Issue: 2. Pages: 314-325. Published: MAY 2010. 356 Self-Reduction of Gold on Activated Carbon Cloth Saipanya Surin and Sarakonsri Thapanee Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Activated carbon cloths (ACCs) were used as a support for preparation of Au nanoparticles. Pretreatment of ACCs was performed by using oxidizing agent HNO(3) in order introduce surface oxygen complex. Au nanoparticles were prepared by impregnation of tetrachloroauric acid (HAuCl(4)) without reducing agents in deinoized water and ethanol. It was found that the preparation in ethanol solution achieved uniformly dispersed Au nanoparticles with diameter 10-100 nm on the support surfaces. X-ray diffraction (XRD) was employed to verify structural characteristics of Au metal adsorption on oxidized ACC surfaces. Morphology and composition were carried out by Scanning electron microscopy (SEM) and Energy dispersice spectroscopy (EDS), respectively. Key Words: CATALYSTS CHIANG MAI JOURNAL OF SCIENCE. Volume: 37 Issue: 1 Pages: 99-105. Published: JAN 2010. 357 Sensor response formula for sensor based on ZnO nanostructures Hongsith Niyom1,2, Wongrat Ekasiddh1,2, Kerdcharoen Teerakiat3 and Choopun Supab1,2 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Higher Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. 3 Department of Physics, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Abstract In this paper, we propose a new and general formula to describe ethanol adsorption mechanism underlying the response enhancement of ZnO nanostructure sensors. The derivation of sensor response formula based on basic chemical reaction at the sensor Surface is presented. The formula can be used to explain response enhancement due to effect of metal doping, surface-to-volume ratio, and surface depletion layer. Thus, it can be regarded as a general formula to describe the sensor response characteristics of ZnO sensors. This general formula is a powerful tool for designing ZnO sensor at my desired sensor response. Furthermore, it is reasonable to expand this formula to explain other sensing materials and also to explain for different active gases. (C) 2009 Elsevier B.V. All rights reserved. Key Words: ETHANOL SENSING CHARACTERISTICS; GAS SENSORS; GROWN ZNO; NANORODS; NANOPARTICLES; FABRICATION; NANOBELTS; FILMS SENSORS AND ACTUATORS B-CHEMICAL. Volume: 144 Issue: 1 Pages: 67-72. DOI: 10.1016/j.snb.2009.10.037. Published: JAN 29 2010. 358 Sequential injection analysis with lab-at-valve (SI-LAV) for the determination of solasodine in Solanum species Thongchai Wisanu1, Liawruangrath Boonsom1,4 and Liawruangrath Saisunee2,3,4 1 Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Institute of Science & Technology Research & Development, Applied Chemistry & Environment Research Unit, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The development of sequential injection analysis with lab-at-valve (LAV) semiautomated system online liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various Solanum species fruits. The main proposed is semi-automated extractive determination of solasodine using methyl orange as colorimetric reagent. After optimization of the system, sample, reagent and organic solvent were sequentially aspirated into an extraction coil connected to the center of a selection valve, where extraction took place by flow reversal. The aqueous and organic phases were separated in a lab-at-valve unit attracted to one of the ports of the selection valve. The absorption of ion-pair solasodine-methyl orange complex in the organic phase was measured spectrophotometrically at 420 nm. The method performances, including reproducibility, linearity, sensitivity and accuracy, were also evaluated. The proposed method is simple, reproducible and accurate. It was successfully applied to the determination of solasodine in Solanum aculeatissimum Jacq.. Solarium violaceum Ortega., Solanum melongena Linn. and Solanum indicum Linn, fruits in Solanaceae family. Results obtained were in good agreement with those obtained by batch wise spectrophotometric method. It is also suitable and useful for determination of solasodine in other medicinal plants. (C) 2009 Elsevier B.V. All rights reserved. Key Words: THIN-LAYER-CHROMATOGRAPHY; NONAQUEOUS CAPILLARY-ELECTROPHORESIS; PERFORMANCE LIQUID-CHROMATOGRAPHY; STEROIDAL ALKALOIDS; FLOW-INJECTION; SOLVENT-EXTRACTION; GAS-CHROMATOGRAPHY; ON-VALVE; GLYCOALKALOIDS; SEPARATION TALANTA Volume: 81 Issue: 1-2 Pages: 565-571. DOI: 10.1016/j.talanta.2009.12.044 Published: APR 15 2010. 359 Sequential Injection-Cation Exchange Micro-column System for Hemoglobin Typing to Differentiate HbE Carriers Hartwell Supaporn Kradtap1,2, Sripaoraya Worathip1,2, Lapanantnoppakhun Somchai1,2, Sanguansermsri Torpong3 and Grudpan Kate1,2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center for Innovation Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A weak cation exchange micro-column was incorporated into a sequential injection (SI) system to perform automatic hemoglobin (Mb) typing as an alternative way to measure HbE. Separation of HbF, HbA and HbA(2)/HbE was performed using phosphate buffer solutions in the pH range of 6 - 7 to create pH gradient mobile phase. The resultant chromatogram showed relative amounts of HbE to other types of hemoglobins in more quantitative detail than the conventional techniques such as dichlorophenol indophenol precipitation and micro-column anion exchange. The system is more economical than a commercially available ion-exchange HPLC analyzer for hemoglobin testing, though analysis time per run is longer due to the aspiration operation of the syringe pump of the SI system. It has been demonstrated that the system can differentiate negative (normal) from positive (HbE carriers) subjects. Key Words: THALASSEMIA; HPLC; VARIANTS ANALYTICAL SCIENCES. Volume: 26 Issue: 3 Pages: 361-365. Published: MAR 2010. 360 Sequential Injection-Immunoassay System with a Plain Glass Capillary Reactor for the Assay of Hyaluronan Hartwell Supaporn Kradtap1, Boonmalai Apichart1, Kongtawelert Prachya2 and Grudpan Kate1 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Biochemistry, Faculty of Science, Thailand Excellence Center for Tissue Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A sequential injection capillary immunoassay system (SI-CI) was developed to automate the multi-step competitive bioassay procedures for hyaluronan (HA), a potential biomarker for liver and cancer diseases. Biotinylated HA-binding proteins (b-HABPs), anti-biotin conjugated with horseradish peroxidase (anti-b-HRP) and substrate 3,3’,5,5’ tetramethylbenzidene (TMB) were employed. An ordinary glass capillary was utilized as a bio-reactor where competitive assay took place. Standard HA was immobilized onto an unmodified glass capillary through simple physical adsorption. This glass capillary is much lower cost compared to a fused-silica capillary, and is well suited for use as part of the tubing of a flow system. Using the proposed system, the average amount of HA in human serums from patients with liver disease and healthy subjects could be differentiated with comparable efficiency as the conventional micro-plate assay (as compared by t-test), with an improved assay time per sample (20 min vs. 5 h), and with approximately 10 times less reagent consumption (10 - 80 mu l vs. 100 - 350 mu l). Key Words: LAB-ON-VALVE; ENZYME-IMMUNOASSAY; CHEMILUMINESCENT IMMUNOSENSOR; ELECTROCHEMICAL DETECTION; IMPRINTED POLYMER; FLOW IMMUNOASSAY; SERUM; ACID; MEMBRANE; ANTIBODY ANALYTICAL SCIENCES. Volume: 26 Issue: 1 Pages: 69-74 Published: JAN 2010. 361 Short Communication: Hematological Safety of Perinatal Exposure to Zidovudine in Uninfected Infants Born to HIV Type 1-Infected Women in Thailand Briand Nelly1,2, Le Coeur Sophie2,3,4, Jourdain Gonzague2,3,5, Hotrawarikarn Somboon2,6, Sirinontakan Surat2,7, Hinjiranandana Temsiri2,8, Kanjanavanit Suparat2,9, Traisathit Patrinee10,2, McIntosh Kenneth11,5 and Lallemant Marc2,3,5 1 INED, Paris, France. IRD, UMI 174, Chiang Mai, Thailand. 3 Faculty of Associated Medical Science, Chiang Mai University, Chiang Mai 50000, Thailand. 4 UMR Paris Descartes IRD INED, CEPED, Paris, France. 5 Harvard University, School of Public Health, Department of Immunology & Infectious Diseases, Boston, MA 02115 USA. 6 Klaeng Hospital, Rayong, Thailand. 7 Health Promotion Hospital Regional Center I, Bangkok, Thailand. 8 Somdej Pranangchao Sirikit Hospital, Chon Buri, Thailand. 9 Nakornping Hospital, Chiang Mai, Thailand. 10 Department of Satatistics, Faculty of Science, Chiang Mai University, Chiang Mai 50000, Thailand. 11 Harvard University, School of Medical, Department of Pediatrics, Boston, MA 02115 USA. 2 Abstract The evolution of hematological parameters in HIV-1-exposed uninfected infants according to various durations of perinatal zidovudine exposure was studied. We used data prospectively collected among 1122 HIV-uninfected formula-fed infants born to HIV-infected mothers who participated in a clinical trial to prevent perinatal transmission in Thailand (PHPT-1). Infants were exposed to different durations of zidovudine both in utero and after birth. Hemoglobin level and leukocyte, absolute neutrophil, and lymphocyte counts were measured at birth and at 6 weeks of age. The association between hematological parameters at birth and the duration of zidovudine exposure in utero was studied using a linear regression model, and 362 changes between birth and 6 weeks of age and the duration of postnatal zidovudine exposure using mixed effects models. At birth, the hemoglobin level was lower in newborns exposed to zidovudine for more than 7.5 weeks in utero (adjusted regression coefficient: -0.6 g/dl; 95% confidence interval: -1.1 to -0.1). Six weeks after birth, the hemoglobin level had decreased faster in infants administered zidovudine for more than 4 weeks (adjusted regression coefficient: -0.1 g/dl; 95% confidence interval: -0.2 to -0.1). The duration of perinatal zidovudine exposure was not associated with the evolution of leukocyte, neutrophil, and lymphocyte counts. Despite the differences in hemoglobin levels, grade 3 or 4 anemia did not significantly differ by maternal or infant zidovudine duration. The clinical impact appeared modest, but longer exposure may warrant close monitoring Key Words: IMMUNODEFICIENCY-VIRUS TYPE-1; TO-CHILD TRANSMISSION; TRIAL AIDS RESEARCH AND HUMAN RETROVIRUSES. Volume: 26 Issue: 10 Pages: 1163-1166. DOI: 10.1089/aid.2010.0034. Published: OCT 2010. 363 Simulating Pesticide Transport from a Sloped Tropical Soil to an Adjacent Stream Kahl G.1, Ingwersen J.1, Totrakool S.2, Pansombat K.3, Thavornyutikarn P.4 and Streck T.1 1 Institute of Soil Science & Land Evaluation, Biogeophysics Section, University of Hohenheim, D-70593 Stuttgart, Germany. 2 EHWM CU, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Faculty of Agricuture, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Preferential flow from stream banks is an important component of pesticide transport in the mountainous areas of northern Thailand. Models can help evaluate and interpret field data and help identify the most important transport processes. We developed a simile model to simulate the loss of pesticides from a sloped litchi (Litchi chinensis Sonn.) orchard to an adjacent stream. The water regime was modeled with a two-domain reservoir model, which accounts for rapid preferential flow simultaneously with slow flow processes in the sod matrix. Preferential flow is triggered when the topsoil matrix is saturated or the infiltration capacity exceeded. In addition, close to matrix saturation, rainfall events induce water release to the fractures and lead to desorption of pesticides from fracture walls and outflow to the stream. Pesticides undergo first order degradation and equilibrium sorption to soil matrix and fracture walls. The model was able to reproduce the dynamics of the discharge reasonably well (model efficiency [EF] = 0.56). The cumulative pesticide mass (EF = 0.91) and the pesticide concentration in the stream were slightly underestimated, but the deviation from measurement data is acceptable. Shape and timing of the simulated concentration peaks occurred in the same pattern as observed data. While the effect of surface runoff and preferential interflow on pesticide mass transport could not be absolutely clarified, according to Our simulations, most concentration peaks in the stream are caused by preferential interflow pointing to the important role of this flow path in the hilly areas of northern Thailand. Key Words: ECOSYSTEM MANAGEMENT MODEL; PLAIN RIPARIAN SYSTEM; OPEN-FRACTURED SOIL; WATER-FLOW; PREFERENTIAL FLOW; NORTHERN THAILAND; CONCEPTUAL EXAMINATION; RAINFALL VARIABILITY; SOLUTE TRANSPORT; MACROPORE FLOW JOURNAL OF ENVIRONMENTAL QUALITY. Volume: 39 Issue: 1 Pages: 353-364. DOI: 10.2134/jeq2008.0460. Published: JAN-FEB 2010. 364 Sorption Characteristics of Indigo Carmine as a Blue Colorant for Use in One-bath Natural Dyeing Komboonchoo Sunsanee1,2 and Bechtold Thomas1 1 Research Institute of Textile Chemistry Physics, Innsbruck University, A-6850 Dornbirn, Austria. 2 Department of Industrial Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Indigo carmine can be used as model for a blue natural colorant for wool and hair dyeing in a one-bath procedure. The sorption and dyeing properties of indigo carmine on wool are studied as a prerequisite for the successful application in combination with natural dye. To evaluate the suitable conditions for the dyeing process, different pH, dye concentrations and temperatures were studied. The dyeing and sorption characteristics on wool were determined on the basis of spectrophotometry and color measurement. Both high dye sorption and color strength were obtained when dyeing at elevated temperature, e.g. 80 degrees C, and lower pH, e. g. pH 3. However, there is significant potential to apply the dyestuff also at temperatures in the range of 40-60 degrees C and pH values of 4-5. Depending on the dyebath pH the sorption isotherms follow the Langmuir- or Freundlich-type isotherm. At pH 3 and 4 Langmuir isotherms are observed, while at pH 5, 6 and 7 sorption on wool follows the Freundlich isotherm behavior. From the dyeing isotherms the standard affinity (Delta mu) of the dyeing step was determined. At pH 3 the heat of dyeing (Delta H) and entropy of dyeing (Delta S) were calculated as 40.22 kJ mol(-1) and 0.19 kJ mol(-1) K(-1), respectively. Key Words: DE-OILED SOYA; WASTE-WATER; HAZARDOUS DYE; BOTTOM ASH; MALACHITE GREEN; ACID DYES; ADSORPTION; REMOVAL; RECOVERY; THERMODYNAMICS TEXTILE RESEARCH JOURNAL. Volume: 80 Issue: 8 Pages: 734-743. DOI: 10.1177/ 0040517509342319 Published: MAY 2010. 365 Spatial and temporal variations of ambient PM(10)-bound polycyclic aromatic hydrocarbons in Chiang Mai and Lamphun Provinces, Thailand Chantara Somporn1,2, Wangkarn Sunanta1, Sangchan Walaya1 and Rayanakorn Mongkon1 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Environmental Science Program, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Respirable airborne particulate matters (PM(10)) were collected using high volume air samplers for 24 h every three days from four sampling stations in Chiang Mai and Lamphun Provinces, Thailand The maximum PM(10) concentration for each sampling station ranged from 140 to 182 mu g/m(3) PM(10)-bound polycyclic aromatic hydrocarbons (PAHs) were extracted from quartz fiber filters and analysed by gas chromatography-mass spectrometry (GC-MS). Mean concentration of total PAHs was in a range from 1 7 to 12.2 ng/m(3). Ratio of non-carcinogenic and carcinogenic PAHs found was approximately 1:3 or 1:4 depending on sampling sites. Concentrations of most of carcinogenic PAHs were found to be positively correlated to PM(10) concentrations. Seasonal variation of PM(10)-bound PAHs was obviously seen in all sites. The highest concentration was found in dry season (December March). Spatial variation was observed in one sampling site, where PAHs content was significantly higher than other sites. Apart from traffic congestion, this was probably due to specific local activities i.e. biomass burning. Key words: Air pollution; Biomass burning; GC-MS; PAHs; PM(10) DESALINATION AND WATER TREATMENT Volume: 19 Issue: 1-3 Pages: 17-25. DOI: 10.5004/dwt.2010.1891. Published: JUL 2010. 366 Spatial and Temporal Variations of Physical-Chemical Water Quality and some Heavy Metals in Water, Sediments and Fish of the Mae Kuang River, Northern Thailand Chitmanat Chanagun1,2 and Traichaiyaporn Siripen3 1 NCE EHWM, Chulalongkorn University, Bangkok, Thailand. Faculty of Fisheries Technology & Aquatic Resources, Maejo University, Chiang Mai, Thailand. 3 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 4 The International Postgraduate Program in Environmental Management, Graduate School, Chulalongkorn University, Bangkok, Thailand. 2 Abstract The present study was conducted to investigate heavy metal (Cd, Pb & Zn) contaminants in water, sediments and fish of the Mae Kuang River, Northern Thailand during July, 2008-June, 2009 It was found that the worst water qualities in dry seasons were caused by low water flow, municipal effluents and industrial discharges The surface water in the river was classified into class 3-4 referring to medium-fairly clean water used for consumption after special water treatment Pb and Cd in water were below detection limits, while Zn concentrations in water ranged 0 01-0 11 mg L (1) The Pb, Cd and Zn concentrations in sediment were 3 13-27 56, <0 02-0 43 and 3 42-10 32 mg kg (1), respectively No Cd and Pb residues were found in Henicorhynchus siamensis and Puntioplites proctozysion flesh, while the concentrations of Zn in these fish were 4 57-6 58 mg kg (1) On the other hand, Pb and Cd residues in snakehead fish (Channa striata) were <005-2 13 and <0 02-0 24 mg kg (1) wet weight, while the concentrations of Zn in these fish were 3 37-12 19 mg kg (1) This information provides a useful reference in heavy metal contamination in the Mae Kuang River for river management (C) 2010 Friends Science Publishers Key Words: ENGLAND; ESTUARY; TISSUES INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY. Volume: 12. Issue: 6. Pages: 816-820. Published: NOV 2010. 367 Spray Forming of Bulk Ultrafine-Grained Al-Fe-Cr-Ti Banjongprasert C.1,2, Hogg S. C.3, Liotti E.3, Kirk C. A.4, Thompson S. P.5, Mi J.2 and Grant P. S.2 1 Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Materials, University Oxford, Oxford OX1 3PH, England. 3 Department of Materials, University Loughborough, Loughborough LE11 3TU, Leics, England. 4 Department of Chemistry, University Loughborough, Loughborough LE11 3TU, Leics, England. 5 Diamond Light Source, Didcot OX11 0DE, Oxon, England. 2 Abstract An Al-2.7Fe-1.9Cr-1.8Ti alloy has been spray formed in bulk and the microstructure and properties compared with those of similar alloys produced by casting, powder aomization (PA), and mechanical alloying (MA) routes. In PA and MA routes, a nanoscale metastable icosahedral phase is usually formed and is known to confer high tensile strength. Unlike previous studies of the spray forming of similar Al-based metastable phase containing alloys that were restricted to small billets with high porosity, standard spray forming conditions were used here to produce a similar to 98 pct dense 19-kg billet that was hot isostatically pressed (“HIPed”), forged, and/or extruded. The microstructure has been investigated at all stages of processing using scanning electron microscopy (SEM), electron backscatter diffraction (EBSD), and synchrotron X-ray diffraction (XRD) at the Diamond Light Source. Consistent with the relatively low cooling rate in spray forming under standard conditions, the microstructure showed no compelling evidence for the formation of metastable icosahedral phases. Nonetheless, after downstream processing, the spray-formed mechanical properties as a function of temperature were very similar to both PA rapid solidification (RS) materials and those made by MA. These aspects have been rationalized in terms of the typical phases, defects, and residual strains produced in each process route. Key Words: AMORPHOUS PHASE-FORMATION; MECHANICAL-PROPERTIES; THERMAL-STABILITY; HYBRID COMPOSITES; ALLOYS METALLURGICAL AND MATERIALS TRANSACTIONS A-PHYSICAL METALLURGY AND MATERIALS SCIENCE. Volume: 41A Issue: 12 Pages: 3208-3215. DOI: 10.1007/s11661-0100386-0. Published: DEC 2010. 368 Stemphol Galactoside, a New Stemphol Derivative Isolated from the Tropical Endophytic Fungus Gaeumannomyces amomi Jumpathong Juangjun1, Abdalla Muna Ali2, Lumyong Saisamorn1 and Laatsch Hartmut2 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Institute for Organic & Biomology Chemistry, University of Gottingen, D-37077 Gottingen, Germany. 2 Abstract A new stemphol derivative, stemphol 1-O-beta-D-galactopyranoside (1b), together with three known metabolites, stemphol (1a), indole-3-carboxylic acid, and kojic acid, has been isolated from the ethyl acetate extract of cultures of Gaeumannomyces amomi BCC4066, an endophytic fungus found on healthy parts of ginger (Alpinia malaccensis; Thai name Kha-pa). The structure of 1b was established via spectroscopic methods, including 2D NMR measurements and GC/ MS experiments. Key Words: NATURAL-PRODUCTS NATURAL PRODUCT COMMUNICATIONS. Volume: 5 Issue: 4 Pages: 567-570. Published: APR 2010. 369 Stress-dependent dielectric and ferroelectric properties of sol-gel-derived PZT/WO(3) ceramics Sreesattabud Tharathip1, Unruan Muangjai1, Watcharapasorn Anucha1, Jiansirisomboon Sukanda1 and Yimnirun Rattikorn2 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract In this study, PZT/xWO(3) (where x = 0,0.5,1 and 3wt.%) ceramics were prepared from powders derived from a triol sol-gel process. The powders were pressed and sintered at 1100 degrees C for 6 h. Phase characterization by XRD indicated that the concentration of WO(3) affected the content of tetragonal and rhombohedral phases. The ferroelectric and dielectric properties of the ceramics were determined under compressive stress in the range of 0-130MPa. The ferroelectric properties were reduced with increasing stress. Stress-induced domain wall motion suppression and non-180 degrees ferroelectric domain switching processes were responsible for the changes observed for ferroelectric parameters. In addition, the dielectric constant was found to increase with stress, which could be explained by a change in domain structures and the de-aging phenomenon. Key Words: ZIRCONATE-TITANATE CERAMICS; MECHANICAL-PROPERTIES; PZT NANOCOMPOSITES; MICROSTRUCTURE; COMPOSITES PHYSICA SCRIPTA. Volume: T139 Article Number: 014002. DOI: 10.1088/0031-8949/2010/ T139/014002. Published: MAY 2010. 370 Strong convergence of a new iterative scheme for a finite family of strict pseudo-contractions Kangtunyakarn Atid and Suantai Suthep Department of Mathhematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The purpose of this paper is to introduce a new iterative scheme for finding a common fixed point of a finite family of strict pseudo-contractions. The results obtained in this paper extend and improve the corresponding results of Marino, Colao, Qin and Kang [G. Marino, V. Colao, X. Qin, S. M. Kang, Strong convergence of the modified Mann iterative method for strict pseudo-contractions, Comput. Math. Appl. 57 (2009) 455-465]. (C) 2010 Elsevier Ltd. All rights reserved. Key words: Iterative scheme; Strict pseudo-contractions; S-mapping; Fixed points COMPUTERS & MATHEMATICS WITH APPLICATIONS. Volume: 60 Issue: 3 Pages: 680694. DOI: 10.1016/j.camwa.2010.05.016. Published: AUG 2010. 371 Strong convergence of composite iterative schemes for a countable family of nonexpansive mappings in Banach spaces Klin-eam Chakkrid2 and Suantai Suthep1 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mathematics, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand. Abstract In this paper we propose a new modified viscosity approximation method for approximating common fixed points for a countable family of nonexpansive mappings in a Banach space. We prove strong convergence theorems for a countable family nonexpansive mappings in a reflexive Banach space with uniformly Gateaux differentiable norm under some control conditions. These results improve and extend the results of Jong Soo Jung [J.S. Jung, Convergence on composite iterative schemes for nonexpansive mappings in Banach spaces, Fixed Point Theory and Appl. 2008 (2008) 14 pp., Article ID 167535]. Further, we apply our result to the problem of finding a zero of an accretive operator and extend the results of Kim and Xu [T.H. Kim, H.K. Xu, Strong convergence of modified Mann iterations, Nonlinear Anal. 61 (2005) 51-60], Ceng, et al. [L.-C. Ceng, A.R. Khan, Q.H. Ansari, J.-C, Yao, Strong convergence of composite iterative schemes for zeros of m-accretive operators in Banach space, Nonlinear Anal. 70 (2009) 1830-1840] and Chen and Zhu [R. Chen, Z. Zhu, Viscosity approximation methods for accretive operator in Banach space, Nonlinear Anal. 69 (2008) 1356-1363]. (C) 2010 Elsevier Ltd. All rights reserved. Key words: Viscosity approximation; Strong convergence; Countable family nonexpansive mappings; Composite iterative schemes; Fixed points NONLINEAR ANALYSIS-THEORY METHODS & APPLICATIONS. Volume: 73. Issue: 2. Pages: 431-439. DOI: 10.1016/j.na.2010.03.034. Published: JUL 15 2010. 372 Strong Convergence Theorem for a New General System of Variational Inequalities in Banach Spaces Imnang S.1,2 and Suantai S.1,3 1 Commission on Higher Education, Center of Excellence in Mathematics, Bangkok 10400, Thailand. 2 Department of Mathematics, Faculty of Science, Thaksin University, Phatthalung 93110, Thailand. 3 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract We introduce a new system of general variational inequalities in Banach spaces. The equivalence between this system of variational inequalities and fixed point problems concerning the nonexpansive mapping is established. By using this equivalent formulation, we introduce an iterative scheme for finding a solution of the system of variational inequalities in Banach spaces. Our main result extends a recent result acheived by Yao, Noor, Noor, Liou, and Yaqoob. Key Words: FIXED-POINT PROBLEMS; NONEXPANSIVE-MAPPINGS; EQUILIBRIUM PROBLEMS; MONOTONE MAPPINGS; ITERATIVE METHOD; HILBERT SPACE; SEMIGROUPS FIXED POINT THEORY AND APPLICATIONS. Article Number: 246808. DOI: 10.1155/2010/246808. Published: 2010. 373 STRONG CONVERGENCE THEOREMS BY HYBRID METHODS FOR FAMILIES OF RELATIVELY NONEXPANSIVE MAPPINGS IN HILBERT SPACES Butsan T.2, Dhompongsa S.2 and Takahashi W.1 1 Department of Mathematics & Computer Science, Tokyo Institute of Technology, Tokyo 1528552, Japan. 2 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In 2008, Takahashi, Takeuchi and Kubota [10] proved a strong convergence theorem by the hybrid method for a family of nonexpansive mappings which generalized Nakajo and Takahashi’s theorems [6]. Furthermore, they obtained another strong convergence theorem for the family of nonexpansive mappings by a hybrid method which is different from Nakajo and Takahashi. In this paper, we extend Takahashi. Takeuchi and Kubota’s results for a single relatively nonexpansive mapping or a family of relatively nonexpansive mappings in a Hilbert space. Using these results, we obtain some new strong convergence theorems in a Hilbert space. Key Words: BANACH-SPACES JOURNAL OF NONLINEAR AND CONVEX ANALYSIS. Volume: 11 Issue: 2. Pages: 215227. Published: 2010. 374 Strong convergence theorems for a general system of variational inequality problems, mixed equilibrium problems and fixed points problems with applications Imnang S.2 and Suantai S.1 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mathematics, Faculty of Science, Thaksin University, Phattalung 93110, Thailand. Abstract In this paper, we introduce a new iterative scheme for finding a common element of the set of solutions of a general system of variational inequalities, the set of solutions of a mixed equilibrium problem and the set of common fixed points of a finite family of nonexpansive mappings in a real Hilbert space. Using the demi-closedness principle for nonexpansive mapping, we prove that the iterative sequence converges strongly to a common element of the above three sets under some control conditions. Our main result extends a recent result of Ceng, Wang and Yao [L.C. Ceng, C.Y. Wang and J.C. Yao, Strong convergence theorems by a relaxed extragradient method for a general system of variational inequalities, Math. Meth. Oper. Res. 67 (2008) 375-390]. (C) 2010 Elsevier Ltd. All rights reserved. Key Words: NONEXPANSIVE-MAPPINGS; MONOTONE MAPPINGS; ITERATIVE METHOD MATHEMATICAL AND COMPUTER MODELLING. Volume: 52. Issue: 9-10 . Pages: 16821696. DOI: 10.1016/j.mcm.2010.06.037. Published: NOV 2010 375 STRONG CONVERGENCE THEOREMS FOR MAXIMAL MONOTONE OPERATORS AND GENERALIZED NONEXPANSIVE MAPPINGS IN BANACH SPACES Inthakon W.1, Dhompongsa S.1 and Takahashi W.2,3 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Mathematics & Computer Science, Tokyo Institute of Technology, Meguro Ku, Tokyo 1528552, Japan. 3 Department of Applied Mathematics, National Sun Yat Sen University, Kaohsiung, Taiwan. Abstract In this paper, we prove strong convergence theorems by two hybrid methods for finding a common element of the set of zero points of a maximal monotone operator and the set of fixed points of a generalized nonexpansive mapping in a Banach space. Using these results, we obtain new convergence results for resolvents of maximal monotone operators and for generalized nonexpansive mappings in a Banach space. Key Words: PROXIMAL POINT ALGORITHM; HILBERT-SPACES; HYBRID METHODS; FIXED-POINTS; RESOLVENTS JOURNAL OF NONLINEAR AND CONVEX ANALYSIS Volume: 11 Issue: 1. Pages: 45-63 Published: 2010. 376 Sub-coercive field dynamic hysteresis in morphotropic phase boundary composition of Pb(Zr(1/2)Ti(1/2))O(3)-Pb(Zn(1/3) Nb(2/3))O(3) ceramic and its scaling behavior Wongdamnern N.1, Triamnak N.1, Unruan M.1, Kanchiang K.1, Ngamjarurojana A.1, Ananta S.1, Laosiritaworn Y.1 and Yimnirun R.2 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Institute of Science, School of Physics, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Abstract Sub-coercive field dynamic ferroelectric hysteresis of a morphotropic phase boundary composition of the PZT-PZN ceramic was investigated under influence of the compressive stress. The scaling relation of hysteresis area < A > against frequency f. field amplitude E(0), and stress sigma took a form of < A - A(sigma=0)> alpha f(-0.33)E(0)(3.75)sigma(0.32), which is not different significantly to that of other PZT-PZN compositions with pure tetragonal or rhombohedral structure, as well as to that of soft and hard PZT bulk ceramics. This study suggested that the domain structures, not ceramic compositions, played a key role in controlling dynamic hysteresis behavior of ferroelectric materials. (C) 2009 Elsevier B.V. All rights reserved. Key Words: FERROELECTRIC THIN-FILMS; ELECTROMECHANICAL PROPERTIES; UNIAXIAL-STRESS; SOFT PZT; COMPRESSIVE STRESS; ELECTRIC-FIELD; POLARIZATION; SYSTEMS; MODEL; DEPENDENCE PHYSICS LETTERS A Volume: 374 Issue: 3 Pages: 391-395. DOI: 10.1016/j.physleta. 2009.11.019. Published: JAN 4 2010. 377 Sulfur hexafluoride plasma surface modification of Gly-Ala and Ala-Gly as Bombyx mori silk model compounds: Mechanism investigations Sangprasert W.1, Lee V. S.1, Boonyawan D.2, Tashiro K.3 and Nimmanpipug P.1 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Graduate School of Engineering, Department of Future Industry Oriented Basic Science & Materials, Tempaku Ku, Toyota Technological Institute, Nagoya, Aichi 4688511, Japan. Abstract Low-pressure plasma has been used to improve the hydrophobicity of Thai silk. In this study, Glycine-Alanine (GA) and Alanine-Glycine (AG) were chosen to represent model compounds of Bombyx mori silk. Single crystals of the simplified model compounds were characterized by polarizing microscopy and X-ray diffraction. The space groups of P2(1)2(1)2(1) and P2(1) were found for AG and GA, respectively. The initial structures for calculation were obtained from the experimental crystal structures. Density functional theory at the BH and HLYP levels was used to investigate possible mechanisms of fluorine radicals reacting with AG and GA in the SF(6) plasma treatment. The results indicate that hydrogen atoms of silk model compounds were most likely to be abstracted from the alanine residue. (c) 2009 Elsevier B.V. All rights reserved. Key Words: BOND NETWORK STRUCTURE; L-ALANINE HYDROCHLORIDE; CRYSTAL-STRUCTURE; POLY(M-PHENYLENE ISOPHTHALAMIDE); SF6 PLASMA; DENSITY; SERIES; HYDROPHOBICITY; ALANYLGLYCINE JOURNAL OF MOLECULAR STRUCTURE. Volume: 963 Issue: 2-3 Pages: 130-136. DOI: 10.1016/j.molstruc.2009.10.025. Published: JAN 29 2010. 378 Surface Characterization of PZN Ceramics Prepared by a Columbite B-Site Precursor Srisombat La-Ongnuan1, Ngamjarurojana Athipong2, Yimnirun Rattikorn3 and Ananta Supon2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Institure of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. Abstract Pyrochlore-type PZN ceramics have been obtained from a two-stage process with columbite B-site precursor. In order to determine the chemical composition of these samples, a combination between X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) techniques has been performed. XRD analysis revealed the coexistence of unreacted PbO starting precursor and major phase of the pyrochlore-type PZN phase as average composition from the bulk volume. While different binding energies (multicomponents) of each chemical element e.g. Pb, Zn, Nb and O from the surface of PZN ceramics were identified by XPS, indicating inhomogeneous surface chemical distribution within these columbiteroute samples. Key Words: RAY PHOTOELECTRON-SPECTROSCOPY; NICKEL NIOBATE POWDERS; FERROELECTRIC CERAMICS; CHEMICAL-STATES; PHASE-FORMATION; PARTICLE-SIZE; OXYGEN; XPS FERROELECTRICS. Volume: 405 Pages: 76-81. Article Number: PII 930389692 DOI: 10.1080/00150193.2010.482895. Published: 2010. 379 Synthesis of a Novel Fluorescent Sensor Bearing Dansyl Fluorophores for the Highly Selective Detection of Mercury (II) Ions Wanichacheva Nantanit1, Watpathomsub Supranee1, Lee Vannajan Sanghiran2,3 and Grudpan Kate2,3 1 Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A new macromolecule possessing two dansyl moieties and based on 2-[4-(2-aminoethylthio)butylthio]ethanamine was prepared as a fluorescent sensor and its mercury sensing properties toward various transition metal, alkali, and alkali earth ions were investigated. The designed compound exhibited pronounced Hg(2+)-selective ON-OFF type fluorescence switching upon binding. The new compound provided highly selective sensing to Hg(2+) in acetonitrile-water solvent mixtures with a detection limit of 2.49 x 10(-7) M or 50 ppb. The molecular modeling results indicated that ions-recognition of the sensor originated from a self assembly process of the reagent and Hg(2+) to form a helical wrapping structure with the favorable electrostatic interactions of Hg(2+) coordinated with sulfur, oxygen, nitrogen atoms and aromatic moieties. Key Words: FLUOROIONOPHORIC BEHAVIORS; CATION-BINDING; CHEMOSENSOR; CROWN; HG2+; 8-HYDROXYQUINOLINE; CHROMOIONOPHORE; CARRIER; CYCLAM; ETHERS MOLECULES. Volume: 15 Issue: 3 Pages: 1798-1810. DOI: 10.3390/molecules15031798. Published: MAR 2010. 380 Synthesis of CdIn(2)Se(4) compound used as thermoelectric materials via the solution method Adpakpang Kanyaporn1, Sarakonsri Thapanee1, Isoda Seiji2, Shinoda Yasuhiro2 and Thanachayanont Chanchana3 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Kyoto University, Institute for Chemical Research, Kyoto 611, Japan. 3 National Metal & Materials Technology Center, Klongluang 10120, Pathumthani, Thailand. Abstract CdIn(2)Se(4) semiconductor is known as a high performance electrical material. In this study, CdIn(2)Se(4) powder was synthesized via an aqueous chemical reduction, or a solution method, at low temperature, using Se metal, InCl(3), and CdCl(2)center dot 2.5H(2)O as precursors, NaBH(4) as a reducing agent, and water as a solvent. Preparative parameters have been considered; reaction temperature at 100 and 130 degrees C, reaction time at 30 min and 6 h. Finally, product powders were characterized by X-ray diffraction, scanning electron microscopy and transmission electron microscopy techniques. The CdIn(2)Se(4) phase was observed to occur in some preparative conditions with In(OH)(3) contaminating phase. However, In(OH)(3) impurity has disappeared when the reaction temperature was 130 degrees C with a reaction time of 30 min, confirmed by XRD patterns. Particle size of product powders was measured from TEM micrographs to be 9.17 +/- 0.94 nm. (C) 2010 Elsevier B.V. All rights reserved. Key words: Cadmium indium selenide; Thermoelectric materials; Solution method JOURNAL OF ALLOYS AND COMPOUNDS. Volume: 500 Issue: 2 Pages: 259-263. DOI: 10.1016/j.jallcom.2010.04.023. Published: JUN 25 2010. 381 Synthesis of hexagonal WO(3) nanowires by microwave-assisted hydrothermal method and their electrocatalytic activities for hydrogen evolution reaction Phuruangrat Anukorn1,2, Ham Dong Jin1, Hong Suk Joon1, Thongtem Somchai2 and Lee Jae Sung1 1 Department of Chemical Engineering, School Environmental Science & Engineering, Ecofriendly Catalysis & Energy Laboratory NRL, Pohang University of Science & Technology, Pohang 790784, South Korea. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Hexagonal WO(3) (hex-WO(3)) nanowires with high aspect ratio and crystallinity have been prepared for the first time by a microwave-assisted hydrothermal method. By using X-ray diffraction, scanning electron microscopy,transmission electron microscopy and high resolution transmission electron microscopy, the phase and morphology of the products were identified, which were controlled by reaction temperature, holding time and added salts. Uniform hex-WO(3) nanowires with a diameter of 5-10 nm and lengths of up to several micrometres were synthesized by a microwave-assisted hydrothermal process at 150 degrees C for 3 h in a solution containing (NH(4))(2)SO(4) as a capping reagent and Na(2)WO(4) as a starting material. The aspect ratio and specific surface area of hex-WO(3) nanowires were 625 and 139 m(2) g(-1), respectively, which represented one of the highest values reported for WO(3). The electrocatalytic activity for hydrogen evolution reaction of hex-WO(3) nanowires was also investigated by cyclic voltammetry and linear sweep voltammetry. The results demonstrated that hex-WO(3) nanowires were a promising electrocatalyst for the hydrogen evolution reaction (HER) from water. Key Words: THIN-FILMS; ELECTROCHROMIC DEVICES; TUNGSTEN-OXIDE; NANORODS; GROWTH; WATER; NANOSTRUCTURES; DEPOSITION; CATALYSTS; SYSTEM JOURNAL OF MATERIALS CHEMISTRY. Volume: 20 Issue: 9 Pages: 1683-1690. DOI: 10.1039/b918783a Published: 2010. 382 Synthesis of nanocrystalline metal molybdates using cyclic microwave radiation Phuruangrat A.2, Thongtem T.1 and Thongtem S.2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Nanocrystalline compounds MMoO(4) (M = Ca, Sr and Ba) were synthesized in propylene glycol using cyclic microwave radiation. XRD, TEM, SAED and EDX analyses revealed the presence of nanocrystallites of the phases containing the corresponding alkaline earth metals, Mo and O. The calculated lattice parameters and the crystallite sizes both increased with the increase in the atomic masses and the ionic radii of the divalent metals. Six different vibrations were detected using Raman spectroscopy, and very strong Mo-O stretching mode of [MoO(4)](2-) tetrahedrons was observed using FTIR. Key Words: CITRATE COMPLEX METHOD; ASSISTED SYNTHESIS; ETHYLENEGLYCOL; SPECTRA; CAMOO4; CRYSTALS; SRMOO4 MATERIALS SCIENCE-POLAND. Volume: 28 Issue: 2 Pages: 557-563. Published: 2010. 383 Synthesis of novel ZnS/ZnAl(2)S(4) core/shell nanocomposites using a facile solvothermal route Thongtem Titipun1, Pilapong Chalermchai1 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Novel ZnS/ZnAl(2)S(4) core/shell nanocomposites were successfully synthesized by a surfactant-free solvothermal reaction at 200 degrees C for 10 h. XRD, FESEM, TEM, and photoluminescence (PL) were used to characterize these novel nanocomposites-build-up of zinc blende ZnS nanorod cores and wurtzite ZnAl(2) S(4) shells. PL emission of ZnS nanorod cores were influenced by ZnAl(2)S(4) shells covered around them. A formation mechanism of ZnS/ZnAl(2)S(4) core/shell nanocomposites was also explained to relate with these results. (C) 2010 Elsevier B.V. All rights reserved. Key Words: ZNIN2S4; NANOPARTICLES; PHOTOCATALYST; MORPHOLOGY; NANOBELTS; NANORODS; GROWTH JOURNAL OF ALLOYS AND COMPOUNDS. Volume: 496 Issue: 1-2 Pages: L29-L32. DOI: 10.1016/j.jallcom.2010.02.134 Published: APR 30 2010. 384 Synthesis of zeolite phases from combustion by-products Pimraksa Kedsarin1, Chindaprasirt Prinya2 and Setthaya Naruemon3 1 Department of Industrial Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Civil Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen, Thailand. 3 School of Science & Technology, Naresuan University, Phayao, Thailand Abstract Synthesis of zeolites from combustion by-products, including fly ash, bottom ash and rice husk ash, was studied. A molar ratio of SiO(2)/Al(2)O(3) of 1.5 was used for the syntheses. Refluxing and hydrothermal methods were also used for synthesis for comparison. The reaction temperatures of refluxing and hydrothermal methods were 100 degrees C and 130 degrees C, respectively. Sodalite, phillipsite-K, and zeolite P1 with analcime were obtained when fly ash, bottom ash and rice husk ash were used as starting materials, respectively. With rice husk ash as a starting material, zeolite P1 was produced. This result had advantages over previous studies as there was no prior activation required for the synthesis. The concentrations and types of alkaline used in the synthesis also determined the zeolite type. The different zeolites obtained from three systems were measured for specific surface area and pore size by using BET and Hg-porosimetry, respectively. Ammonium exchange capacities of the synthesised powders containing zeolites, sodalite, zeolite P1 and phillipsite-K were 38.5, 65.0 and 154.7 meq 100 g (1), respectively. Key Words: COAL FLY-ASH; RICE HUSK ASH; HYDROTHERMAL SYNTHESIS; POTENTIAL APPLICATIONS; ACTIVATION; MECHANISM; REMOVAL; ZSM-48 WASTE MANAGEMENT & RESEARCH. Volume: 28. Issue: 12 Pages: 1122-1132. DOI:10. 1177/0734242X09360366. Published: DEC 2010. 385 Synthesis, characterisation and photoluminescence of nanocrystalline calcium tungstate Phuruangrat Anukorn1, Thongtem Titipun2 and Thongtem Somchai1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Calcium tungstate nanocrystalline was successfully synthesised via a microwave irradiation method using Ca(NO3)2 and Na2WO4 in ethylene glycol, without the requirement of any calcination. The product was analysed by X-ray diffraction (XRD), Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and photoluminescence spectrometry. XRD, SAED and TEM revealed that the product was body-centred tetragonal CaWO4 with an average particle size of 12 nm. Different oscillation modes were detected by Raman spectroscopy and FTIR. They showed the strong W-O stretching in [WO4]2- tetrahedrons at 711-933 cm-1. Photoluminescence (PL) of CaWO4 nanocrystalline showed the maximum emission peak at 422 nm. Key Words: MICROWAVE-ASSISTED SYNTHESIS; CAWO4 THIN-FILMS; CA JOURNAL OF EXPERIMENTAL NANOSCIENCE. Volume: 5 Issue: 3 Pages: 263-270. Article Number: PII 923564681. DOI: 10.1080/17458080903513276. Published: 2010. 386 Taxonomic characterization of Streptomyces strain CH54-4 isolated from mangrove sediment Srivibool Rattanaporn2, Jaidee Kanpicha2, Sukchotiratana Morakot1, Tokuyama Shinji3 and Pathom-aree Wasu1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Institute of Marine Science, Burapha University, Chon Buri 20131, Thailand. 3 Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 4228529, Japan. 2 Abstract An actinobacterium, designated as strain CH54-4, was isolated from mangrove sediment on the east coast of the Gulf of Thailand using starch casein agar. This isolate was found to contain chemical markers typical of members of the genus Streptomyces: This strain possessed a broad spectrum of antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. In addition, this strain also showed strong activity against breast cancer cells with an IC(50) value of 2.91 mu g ml(-1). Phylogenetic analysis of a 16S rRNA gene sequence showed that strain CH54-4 forms a distinct clade within the Streptomyces 16S rRNA gene tree and closely related to Streptomyces thermocarboxydus. Key words: Mangrove sediment; Streptomyces; Taxonomy ANNALS OF MICROBIOLOGY. Volume: 60 Issue: 2 Pages: 299-305. DOI: 10.1007/s13213010-0041-4. Published: JUN 2010. 387 The case for the use of unrefined natural reagents in analytical chemistry-A green chemical perspective Grudpan Kate1,2, Hartwell Supaporn Kradtap1,2, Lapanantnoppakhun Somchai1,2 and McKelvie Ian3 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center for Innovation Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Water Studies Center, School Chemistry, Monash University, Clayton, Vic 3800, Australia. Abstract An important part of the Green Chemistry philosophy is the need to develop and adopt green analytical techniques and procedures. These include the reduction of reagent and solvent usage, the minimization of solid, liquid and gaseous materials produced by analytical processes, the replacement of reagents and solvents of high occupational or environmental toxicity with much more innocuous materials, and the reduction of energy use in analytical processes. One aspect that has received little attention in this context is the use of unrefined natural reagents derived from plant and animal tissues or microbial cells. Crude plant extracts may contain chemical compounds that enable their use as indicators in acid-base or redox titrations, or as chromogenic or fluorogenic agents. Enzymes extracted from plants may be used directly in soluble form, or incorporated in biosensors or solid phase reactors, for analytical measurements, or as a means of removing interferences or performing speciation studies. The use of natural reagents in conjunction with a flow injection system can confer a number of advantages. The enhanced kinetic control that flow analysis offers may assist in avoiding undesirable side reactions that would otherwise occur using unpurified reagents. The lifetime of natural reagents may be prolonged when used in a flow analysis system because their exposure to light or air can be controlled. Any changes in response that do occur may be readily corrected by regular standard checking and recalibration. This article reviews the use of natural reagents with an emphasis on flow-based analytical systems, and makes the case for further research in this latent area of green analytical chemistry. Key Words: INJECTION SPECTROPHOTOMETRIC DETERMINATION; BATATAS L. LAM.; SWEET-POTATO TISSUE; CRUDE EXTRACT; ENZYMATIC SOURCE; WASTEWATER; PHOTOGRAPHIC PROCESS; POLYPHENOL OXIDASE; FLOW-ANALYSIS; ANALYTICAL METHODS. Volume: 2. Issue: 11. Pages: 1651-1661. DOI: 10.1039/c0ay00253d Published: NOV 2010. 388 The chemical composition and antioxidant activities of basil from Thailand using retention indices and comprehensive two-dimensional gas chromatography Pripdeevech Patcharee1, Chumpolsri Watcharapong2, Suttiarporn Panawan2 and Wongpornchai Sugunya2 1 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Department of Chemistry, Center of Excellence Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract The chemical compositions of essential oils obtained from Ocimum basilicum var. thyrsiflora (1.39% dry weight) and Ocimum basilicum (0.61%) were analyzed by GC-MS. Seventy-three constituents representing 99.64% of the chromatographic peak area were obtained in the O. basilicum var. thyrsiflora oil, whereas 80 constituents representing 91.11% observed in the essential oil of O. basilicum were obtained. Methyl chavicol (81.82%), beta-(E)-ocimene (2.93%) and alpha-(E)bergamotene (2.45%) were found to be the dominant constituents in O. basilicum var. thyrsiflora oil while O. basilicum contained predominantly linalool (43.78%), eugenol (13.66%) and 1,8-cineole (10.18%). The clear separation of the volatiles in all samples, demonstrated by the application of GCxGC, resulted in significantly different fingerprints for the two types of basil. The O. basilicum oil showed strong antioxidant activity while the oil of O. basilicum var. thyrsiflora exhibited very low activity, which was attributed to the significant differences in linalool and eugenol contents in these essential oils. Key Words: ESSENTIAL OIL COMPOSITION; FLIGHT MASS-SPECTROMETRY; OCIMUM-BASILICUM; CALLOSOBRUCHUS-MACULATUS; VOLATILE COMPONENTS; L.; LEAVES; GRATISSIMUM; VARIABILITY; CULTIVARS JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. Volume: 75. Issue: 11. Pages: 15031513. DOI: 10.2298/JSC100203125P. Published: 2010. 389 The Debye Dielectric Behavior of Mixed Normal and Relaxor-Ferroelectrics: Monte Carlo Investigation Laosiritaworn Yongyut1,2,3, Kanchiang Kanokwan1,2, Ngamjarurojana Athipong1,2, Yimnirun Rattikorn4, Guo Ruyan5 and Bhalla Amar S.5 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Thailand Center of Excellence in Physics, Commission on Higher Education, Bangkok 10400, Thailand. 3 Science & Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Institute Science, School of Physics, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. 5 Department of Electrical & Computer Science, University of Texas at San Antonio, San Antonio, TX 78249 USA. Abstract In this work, Monte Carlo simulation was used to investigate the Debye dielectric behavior of mixed normal- and relaxor-ferroelectrics. The results show that, with increasing temperature, the dielectric permittivity of the mixed system sharply increases at low temperatures but slowly decays at high temperatures, while still presenting the dielectric peaks close to the relaxor- and normal-ferroelectrics transition temperatures. Further, it is found that the sharpness, the magnitude, and the frequency dispersion of the dielectric peaks can be finely tuned by adjusting relevant normal-ferroelectric parameters in the model. However, the modified Curie-Weiss law shows that its exponent is governed by the inversetemperature dependence as the exponent is very close to 2 regardless of normalferroelectric content. The functionalities of the Debye-type dielectric permittivity are then discussed along with the comparison with experiments. Key Words: TECHNOLOGY; CERAMICS FERROELECTRICS. Volume: 401. Pages: 239-245. Article Number: PII 930375294. DOI: 10.1080/00150191003677114. Published: 2010. 390 The effect of H(2)O and PEG on the morphologies of ZnO nanostructures synthesized under microwave radiation Thongtem Titipun1, Jattukul Siriprapha1, Phuruangrat Anukorn2 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract ZnO nanoparticles and nanorods were synthesized from Zn(NO(3))(2)center dot 6H(2)O and NaOH in H(2)O and in different molecular weights (MWs) of polyethylene glycol (PEG) using 180W microwave radiation. The phase was detected using X-ray diffraction (XRD) and selected area electron diffraction (SAED). The diffraction pattern was in accordance with that obtained by simulation. Scanning and transmission electron microscopy (SEM and TEM) revealed the presence of nanoparticles with an average size of 12.7 +/- 2.7 nm in water; these gradually changed into nanorods in PEG with different MWs. Four Raman vibrations were detected, with the strongest intensity at 439cm(-1), specified as the optical phonon E(2H) high frequency mode. Luminescence emission was detected at 366-374 nm, caused by the recombination of free excitons in the products. Emission intensity was highest for the product synthesized in PEG600. (C) 2009 Elsevier B.V. All rights reserved. Key Words: LOW-TEMPERATURE; HYDROTHERMAL SYNTHESIS; NANORODS; NANOPARTICLES; FABRICATION; FLOWERS; ARRAYS; ROUTE JOURNAL OF ALLOYS AND COMPOUNDS. Volume: 491 Issue: 1-2 Pages: 654-657. DOI: 10.1016/j.jallcom.2009.11.033. Published: FEB 18 2010. 391 The Effects of Sugar Application on the Concentrations of Anthocyanin and Flavonol of ‘Mahajanaka’ Mango (Mangifera indica Linn. cv. Mahajanaka) Fruit Lueangprasert Kanyarat1, Uthaibutra Jamnong1, Saengnil Kobkiat1 and Arakawa Osamu2 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Faculty of Agricuture & Life Science, Hirosaki University, Aomori 0368561, Japan. Abstract The relationship between contents of anthocyanin, flavonol, sugars and sunlight were explored in Mahajanaka mango (Mangifera indica Linn. cv. Mahajanaka) fruit exocarp in Thailand. Sugars including fructose (5 or 10% weight/volume; w/v) and sucrose (10% w/v) were applied three times at 77, 84 and 91 days after full bloom before red colour development. Anthocyanin and flavonol contents increased throughout fruit development, especially after the application of 10% fructose with a greater response in the sunny exposed sections of the fruit than in shaded sections. The concentrations of sucrose and fructose were generally higher in the exposed sections after applications of the sugars compared with the control. There was a positive correlation between anthocyanin and flavonol contents across the sugar treatment in the exposed side of the fruit. However, there were strong correlations between the concentrations of anthocyanin and sugars in the shaded side of the fruit. These results suggest a strong relationship between colour development, light and exposure to exogenous sugars in this mango cultivar. Key Words: GENE-EXPRESSION; APPLE FRUIT; LIGHT; SKIN; BIOSYNTHESIS; COLOR; ACCUMULATION; PATHWAY CHIANG MAI JOURNAL OF SCIENCE. Volume: 37 Issue: 2. Pages: 355-362. Published: MAY 2010. 392 The Fixed Point Property of Unital Abelian Banach Algebras Fupinwong W. and Dhompongsa S. Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract We give a general condition for infinite dimensional unital Abelian Banach algebras to fail the fixed point property. Examples of those algebras are given including the algebras of continuous functions on compact sets. Key Words: SPACES FIXED POINT THEORY AND APPLICATIONS. Article Number: 36282. DOI: 10.1155/ 2010/362829. Published: 2010. 393 The gas phase conformers and vibrational spectra of valine, leucine and isoleucine: An ab initio study Dokmaisrijan Supaporn1, Lee Vannajan Sanghiran2,3 and Nimmanpipug Piyarat2,3 1 School of Science, Division of Chemistry, Walailak University, Nakhon Si Thammarat 80161, Thailand. 2 Center for Innovation Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, CSML, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The present work reports the results of a conformational study performed on three essential non-polar amino acids: valine, leucine and isoleucine. Ab initio calculations were done at the MP2/6-311++G(2d,2p) level in the gas phase. The 6, 8 and 12 conformers served as a basis to represent the shapes of valine, leucine and isoleucine in the gas phase, respectively, with the relative energies (Delta E(Total)) below 1,25 kcal mol(-1) compared to the most stable conformer of each amino acid. The Delta E(Total) values are reported at the MP2/6-311++G(2d,2p) level with corrections for zero-point vibrational energies. The lowest energy conformers of each amino acid contained the intramolecular hydrogen bond (H-bond) interactions between the NH(2) and COOH groups which are the N-H center dot center dot center dot O = C, N-H center dot center dot center dot O-H and O-H center dot center dot center dot N-H H-bonds. It is obvious that the H-bond interaction plays an important role in the conformational energy and harmonic vibrational frequencies. From the theoretical vibrational spectra, the O-H out of plane and in-plane bending shift ranged from similar to 1100 cm(-1) to similar to 1360 cm(1) and the O-H stretching shifts from similar to 3600 cm(-1) to similar to 3400 cm(-1) due to the O-H center dot center dot center dot N-H H-bond interaction. In addition, the side-chain orientations can also affect the conformational stability and diversity, resulting in a large number of low energy conformational minima of amino acid. (C) 2010 Elsevier B.V. All rights reserved. Key words: Valine; Leucine; Isoleucine; Conformer; Conformational study; Vibra- JOURNAL OF MOLECULAR STRUCTURE-THEOCHEM Volume: 953. Issue: 1-3. Pages: 28-38. DOI: 10.1016/j.theochem.2010.04.033. Published: AUG 15 2010. 394 The Interactive Effects of Inorganic Carbon and Light on a Tropical Submerged Macrophyte: Egeria densa Planchon (Hydrocharitaceae) Jampeetong Arunothai Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The interactive effects of carbon dioxide (CO(2)) and bicarbonate (HCO(3) (-)) on a tropical submerged species: Egeria densa Planchon (Hydrocharitaceae) was studied. A factorial combination of two concentrations of CO(2) (370 ppm and 2,000 ppm) and three concentrations of HCO(3)(-) (0.1 mM, 0.85 mM and 1.7 mM) were set at the same condition of light intensities (300 mu mol m(-2) s(-1)) and temperature (23 degrees C). The relative growth rate (RGR) and some morphological characteristics were measured after 14 days. A significant difference in RGR, shoot growth rate based on shoot length (SGR), number of new roots, and new shoots between plants treated by low and high CO(2) concentration were observed. The results indicate that the concentrations of both CO(2) and HCO(3) (-) affected growth. At low concentrations of CO(2) and HCO(3)(-) there was the lowest RGR, growth rate, and production of new shoots and roots. At the high CO(2) concentration, the results showed the growth of plants will decrease when the HCO(3)(-) concentration was low. The ability of E. densa to use HCO(3)(-) as an inorganic carbon source for photosynthesis was studied by a pH-drift experiment. Small E. densa shoots (1-1.5 cm long) were incubated in 30 ml glass-stoppered bottles with a growth medium containing 0.8 mmol l(-1) of HCO(3)(-) at 23 degrees C and 300 mu mol m(-2)s(-1) of light for 20 hours. The final pH, which was 10.1310.78, showed that E. densa can take up HCO(3)(-), after an attended treatment at a high CO(2) concentration. Moreover, the effect of HCO(3)(-) on photosynthetic oxygen production was studied at low (50 mu mol m(-2)s(-1)) and high (300 mu mol m(-2)s(-1)) light intensities. The rate of photosynthesis increased when the concentration of HCO(3)(-) was increased. Furthermore, the photosynthetic rate at the high light intensity condition was 10 times greater than at the low light intensity condition. Key words: Egeria densa; bicarbonate user; photosynthesis; inorganic carbon CHIANG MAI JOURNAL OF SCIENCE. Volume: 37. Issue: 3. Pages: 528-535. Published: SEP 2010. 395 The occurrence of Melissococcus plutonius in healthy colonies of Apis mellifera and the efficacy of European foulbrood control measures Budge Giles E.1, Barrett Ben1, Jones Ben1, Pietravalle Stephane1, Marris Gay1, Chantawannakul Panuwan2, Thwaites Richard1, Hall Jayne1, Cuthbertson Andrew G. S.1 and Brown Mike A.1 1 Food & Environment Research Agency, York YO41 1LZ, New Yorkshire, England. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Abstract European foulbrood (EFB) persists in England and Wales despite current treatment methods, all of which include feeding honey bee colonies with the antibiotic oxytetracycline (OTC). A large-scale field experiment was conducted to monitor a husbandry-based method, using comb replacement (known as Shook swarm), as a drug free EFB control option. The understanding of EFB epidemiology is limited, with little information on the presence of Melissococcus plutonius in disease free colonies. Additional samples were collected from diseased and disease free apiaries to identify symptomless infection. EFB reoccurrence was not significantly different between OTC and husbandry methods and real-time PCR data demonstrated that fewer Shook swarm treated colonies contained M. plutonius carryover to the Spring following treatment. Asymptomatic colonies from diseased apiaries showed an increased risk of testing positive for M. plutonius compared to asymptomatic colonies from disease free apiaries. The probability of a sample being symptomatic increased when a greater quantity of M. plutonius was detected in adult bees and larvae. The possibility of treating EFB as an apiary disease rather than a colony disease and the implications of a control strategy without antibiotics are discussed. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved. Key words: Honey bee; Disease control; qPCR; Epidemiology; Detection; Oxytetracycline; Treatment JOURNAL OF INVERTEBRATE PATHOLOGY. Volume: 105. Issue: 2. Pages: 164-170. DOI: 10.1016/j.jip.2010.06.004. Published: OCT 2010. 396 The solution route synthesis of the Cu(0.5)Ag(1.5)InSe(3) compound for thermoelectric applications Chansaenpak Kantapat and Sarakonsri Thapanee Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Cu(0.5)Ag(1.5)InSe(3) compound which is used as a thermoelectric material was successfully synthesized by a simple solution route. The metal ions were reduced simultaneously under alkaline conditions by NaBH(4). The types of solvent, reaction temperature, indium precursors, and reaction time were studied as the parameters of the synthesis method. The prepared powders were investigated by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDS). The results show that the reaction temperature is the main factor affecting Cu(0.5) Ag(1.5)InSe(3) formation. Finally, suitable conditions to prepare Cu(0.5)Ag(1.5) InSe(3) particles were ethylene glycol as a solvent, 200 degree Celsius as a reaction temperature, indium nitrate as an indium precursor, and 72 h as a reaction time. Key Words: AQUEOUS CHEMICAL-SYNTHESIS; DEVICES; MERIT JOURNAL OF CERAMIC PROCESSING RESEARCH. Volume: 11. Issue: 6. Pages: 742-746 Published: DEC 2010. 397 The spitting spider family Scytodidae in Thailand, with descriptions of three new Dictis species (Araneae) Dankittipakul Pakawin and Singtripop Tippawan Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The spitting spider family Scytodidae in Thailand, with descriptions of three new Dictis species (Araneae). - Nine spider species belonging to the genus Dictis L. Koch and presumably to the genus Scytodes Latreille were collected from forests of Thailand. Three of them are new (Dictis elongata sp. n., D. denticulata sp. n., and D. thailandica sp. n.), two are known species for which morphological variation and extended geographic ranges are recorded (Scytodes fusca and S. mawphlongensis), and a further four species known only from one sex are illustrated and provisionally described using open nomenclature (three Dictis and one Scytodes species). REVUE SUISSE DE ZOOLOGIE. Volume: 117. Issue: 1. Pages: 121-141. Published: MAR 2010. 398 Theoretical Description of Electromagnetic Nonbonded Interactions of Radical, Cationic, and Anionic NH(2)BHNBHNH(2) Inside of the B(18)N(18) Nanoring Monajjemi M.5, Lee V. S.1,2, Khaleghian M.3, Honarparvar B.5 and Mollaamin F.4 1 Department of Chemistry, CSML, Faculty of Science, Thailand Center of Excellence in Physics (ThEP), Chiang Mai University, Chiang Mai 50200, Thailand. 2 Faculty of Science, Thailand Center of Excellence in Physics (ThEP), Center for Innovation in Chemistry, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Chemistry, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran. 4 Department of Chemistry, Qom Branch, Islamic Azad University, Qom, Iran. 5 Department of Chemistry, Science & Research Branch, Islamic Azad University, Tehran, Iran. Abstract The electromagnetic nonbounded interactions of the NH(2)BHNBHNH(2) molecule inside of the B(18)N(18) ring have been investigated with hybrid density functional theory (B3LYP) using the EPR-III and EPR-II basis sets for a physicochemical explanation of electromagnetic nonbounded interactions within these nanosystems. Optimized structures and hyperfine spectroscopic parameters such as total atomic charges, spin densities, electrical potential, and isotropic Fermi coupling constants of radical, cationic, and anionic forms of the NH(2) BHNBHNH(2) molecule in different loops and bonds of the B(18)N(18)-NH(2) BHNBHNH(2) systems have been calculated. The correlations between structural, electronic, and spectral properties have been contributed to identify the characteristics of hyperfine electronic structure. Besides structural characteristics, the lowest unoccupied molecular orbital and the highest occupied molecular orbital for the lowest energy have been derived to examine the structural stability of the B(18)N(18)-NH(2)BHNBHNH(2) systems. We have also carried out the calculation for the alanine-glycine amino acids coupled with the NH(2)BHNBHNH(2) molecule inside of the B(18)N(18) ring (ALA-NH(2)BHNBHNH(2)-GLY) and obtained quantized transitional frequencies among the forms of radical, anionic, and cationic. In a similar way, in B(18)N(18)-NH(2)BHNBHNH(2), the three frequencies have been yielded as nu(r-c) = 486948.498 GHz, nu(a-c) = 1792900.812 GHz, and nu(r-a) = 399 2507076.816 GHz. It can be seen that all observed frequencies appeared in the IR and macrowave regions. It seems that the B(18)N(18)-NH(2)BHNBHNH(2) nonbonded system can be used for the measurement of rotational spectra related to electrical voltage differences existing in a part of biomacromolecules. The radial coordinate of the dipole moment vector (r) as well as the voltage differences (Delta V) and relative energies (Delta E) of the radical, anionic and cationic forms of the NH(2) BHNBHNH(2) in the B(18)N(18)-NH(2)BHNBHNH(2) system exhibited Gaussian distribution. The expectations of the Delta E and Delta V and r have been calculated from the Gaussian curves, which have been fitted by various eigenvalues. In addition, the natural bond orbital (NBO) analysis has been performed, which was informative to reveal some important atomic and structural features. Also, analysis of the NQR hyperfine structure of the B(18)N(18)-NH(2)BHNBHNH(2) system has been performed in terms of the electric field gradient at each nitrogen nucleus, and the changes in the extent of electric charge distribution that accompanies complex formation have been explored. JOURNAL OF PHYSICAL CHEMISTRY C. Volume: 114. Issue: 36. Pages: 15315-15330 DOI: 10.1021/jp104274z. Published: SEP 16 2010. 400 Theoretical Study of the Bombyx mori Silk Surface Functionalization: Quantum Mechanical Calculation of the Glycine-Alanine Unit Reacting with Fluorine and Molecular Dynamic Simulation of Wettability Khomhoi Padungsee, Sangprasert Waleepan, Lee Vannajan S. and Nimmanpipug Piyarat 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract SF(6) plasma has been used to improve the hydrophobic properties of Thai Silk. In this study, possible reactions were inveatigated via a glycine-alanine (GA) model; the main component that dominates intermolecular interactions reflecting the physical properties of solk. Quantum mechanical (QM) calculations using density functional theory (DFT) and molecular dynamic (MD) simulations were utilized to investigate possible mechanisms for the interaction between GA, fluorine radicals (F*) and fluorine anions (F(-)). The hydrogen abstarction reactions of radicals are the lowest activation energy pathways and should be the most preferable pathway in the plasma treatment process. From the MD simulation, the interaction energies of water with the silk surface and irradiated surface were -4.65 and -2.63 kcal/ (mole of water), respectively. Key Words: PLASMA TREATMENT; WETTING BEHAVIOR; SF6 PLASMA; AIR PLASMA; FIBERS; HYDROPHOBICITY; IMPROVEMENT; ADHESION CHIANG MAI JOURNAL OF SCIENCE. Volume: 37. Issue: 1. Pages: 106-115. Published: JAN 2010. 401 Thermal analysis and microstructure of Portland cement-fly ash-silica fume pastes Chaipanich Arnon and Nochaiya Thanongsak Cement & Concrete Research Laboratory, Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Thermal analysis (thermogravimetry and differential thermal analysis) was used with scanning electron microscopy technique to investigate the hydration mechanisms and the microstructure of Portland cement-Fly ashsilica fume mixes. Calcium silicate hydrate (C-S-H), ettringite, gehlenite hydrate (C(2)ASH(8)), calcium hydroxide (Ca(OH)(2)) and calcium carbonate (CaCO(3)) phases were detected in all mixes. In the mixes with the use of silica fume addition, there is a decrease in Ca(OH)(2) with increasing silica fume content at 5 and 10% compared to that of the reference Portland-fly ash cement paste and a corresponding increase in calcium silicate hydrate (C-S-H). Key Words: HIGH-STRENGTH CONCRETE; MINERAL ADMIXTURES; CORROSION; HYDRATION JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY. Volume: 99. Issue: 2. Pages: 487-493. DOI: 10.1007/s10973-009-0403-y. Published: FEB 2010. 402 Thermal expansion behavior and estimated total polarizations of lead zirconate titanate-lead nickel niobate ceramics Unruan Muangjai1, Prasatkhetragarn Anurak2, Laosiritaworn Yongyut1, Ananta Supon1, Khamman Orawan1, Yimnirun Rattikorn3, Guo Ruyan4 and Bhalla Amar4 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Materials Science, School of Science & Technology, Naresuan University Phayao, Phayao, Thailand. 3 Institute of Science, School of Physics, Suranaree University Technology, Nakhon Ratchasima 30000, Thailand. 4 Department of Electrical & Computer Engineering, College of Engineering, University of Texas San Antonio, San Antonio, TX 78249 USA. Abstract Thermal expansion properties of lead zirconate titanate-lead nickel niobate ((1-x)PZT-xPNN, x=0.1-0.5) ceramics were determined in the temperature range of approximately -100 to + 450 degrees C by dilatometer thermal expansion measurement. The observed deviation from the straight line below a Bums-like temperature for all the compositions was interpreted as due to the contribution from the dynamic polarization fluctuations of the relaxor part of the compositions. The Burns temperature was determined and found to increase with increasing PZT concentration. The local polarization was calculated from the thermal expansion data. Various aspects of understanding the polarization behavior and other effects in this ferroelectric system were also examined and discussed. (C) 2010 Elsevier B.V. All rights reserved. Key words: Thermal expansion; Polarization; Relaxor ferroelectric; PZT-PNN MATERIALS LETTERS Volume: 64. Issue: 18. Pages: 1960-1963. DOI: 10.1016/j.matlet.2010.06.020. Published: SEP 30 2010. 403 Thermal sprayed stainless steel/carbon nanotube composite coatings Kaewsai D.1, Watcharapasorn A.1, Singjai P.1, Wirojanupatump S.2, Niranatlumpong P.3 and Jiansirisomboon S.1 1 Chiang Mai University, Faculty of Science, Department of Physics & Materials Science, Chiang Mai 50200, Thailand. 2 Chiang Mai University, Faculty of Science, Department of Chemistry, Chiang Mai 50200, Thailand. 3 National Metal & Materials Technology Center, Pathum Thani 12121, Thailand. Abstract Stainless steel/carbon nanotube (SS/CNT) composite coating was prepared by thermal spray from the feedstock powder synthesized by chemical vapor deposition at a synthesis temperature and time of 800 degrees C and 120 min under ethanol atmosphere. Microstructural investigation by TEM and SEM revealed that grown CNTs covering the surface of stainless steel particles were multi-walled type with an average diameter of about 44 nm. Microstructures of pure stainless steel and SS/CNT composite coatings similarly showed splat characteristic and lamellar structure. Incorporation of CNTs was clearly observed in the composite coating. Hardness of SS/CNT composite coating (480 +/- 36 HV(0.3)) was higher than that of pure stainless steel coating (303 +/- 33 HV(0.3)). Coefficient of friction of the SS/CNT coating was almost 3 times lower than that of stainless steel coating which resulted in reduction of sliding wear rate of nearly 2 times. This research thus demonstrated a new composite coating with better wear resistive performance compared to a coating deposited by commercially available stainless steel powder. (C) 2010 Elsevier B.V. All rights reserved. Key Words: REINFORCED ALUMINUM NANOCOMPOSITE; CHEMICAL-VAPORDEPOSITION; CARBON NANOTUBES; MECHANICAL-PROPERTIES; MATRIX NANOCOMPOSITES; ELECTRICAL-PROPERTIES; WEAR-RESISTANCE; NANOCRYSTALLINE; GROWTH 404 TPA-ZrO(2)-SiO(2)-Catalyzed Polyesterification of TMP Polyester Fang Li1,2, Geng Xiang-Dong1, Liu Diansheng1, Gao Jian1, He Li-Juan1, Hao Ya-Juan1 and Saipanya Surin3 1 School of Chemistry & Chemical Engineering, Shanxi University, Taiyuan 030006, People’s Republic of China. 2 Engineering Research Center for Fine Chemical, Ministry of Education, Taiyuan 030006, People’s Republic of China. 3 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A series of tungstophosphoric acid (TPA)-ZrO(2)-SiO(2) particles (30 wt% TPA) with various ZrO(2) contents were prepared by sol-gel method. Using heptanoic acid (HA) as an end-capping agent, trimethylolpropane (TMP) polyester plasticizers were prepared by the TPA-ZrO(2)-SiO(2)-catalyzed polyesterification of TMP and adipic acid (AA). The particles were characterized by means of inductively coupled plasma atomic emission spectroscopy (ICP-AES), X-ray diffraction (XRD), fourier transform infrared (FT-IR), ultraviolet-visible diffuse reflectance (UV-VIS DR) and transmission electron microscopy (TEM). TPA with 200 nm microspheric particles were mostly embedded into the network of ZrO(2)-SiO(2) mixed oxide. Mole ratio of ZrO(2):SiO(2) was 3:7 resulted in the most suitable composition for the catalyzed polyesterification. Excess ZrO(2) induced destruction of the ZrO(2)-SiO(2) framework whilst some TPA only adsorbed on the support surfaces. TPA-3Z-7S exhibited high catalytic activity and better controllable of the weight average molecular weight (MW) distribution of the TMP polyesters. Under the optimal reaction condition the TMP polyester with MW between ca. 600-3800 was achievable prepared with excellent yield (up to 93%). Key words: TPA-ZrO(2)-SiO(2); sol-gel method; TMP polyester; polyesterification CHIANG MAI JOURNAL OF SCIENCE. Volume: 37. Issue: 3. Pages: 397-409. Published: SEP 2010. 405 Tris(ethylenediamine) cobalt(II) sulfate Yotnoi Bunlawee, Seeharaj Athittaya, Chimupala Yothin and Rujiwatra Apinpus Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The structure of the title compound, [Co(II)(C(2)H(8)N(2))(3)]SO(4), the cobalt example of [M(C(2)H(8)N(2))(3)]SO(4), is reported. The Co and S atoms are located at the 2d and 2c Wyckoff sites (point symmetry 32), respectively. The Co atom is coordinated by six N atoms of three chelating ethylenediamine molecules generated from half of the ethylenediamine molecule in the asymmetric unit. The O atoms of the sulfate anion are disordered mostly over two crystallographic sites. The third disorder site of O (site symmetry 3) has a site occupancy approaching zero. The H atoms of the ethylenediamine molecules interact with the sulfate anions via intermolecular N-H center dot center dot center dot O hydrogen-bonding interactions. ACTA CRYSTALLOGRAPHICA SECTION E-STRUCTURE REPORTS ONLINE. Volume: 66 Pages: M628-U333. DOI: 10.1107/S1600536810016168. Part: Part 6. Published: JUN 2010 406 TWO-STEP SYNTHESIS OF CdS (hcp) NANORODS USING SONOCHEMICAL AND HYDROTHERMAL PROCESSES Phuruangrat Anukorn1, Thongtem Titipun2 and Thongtem Somchai1 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract CdS (hcp) crystals were synthesized using CdCl(2 center dot)2H(2)O in C(4) H(11)N containing 20 % (NH(4))(2)S by the 5 h sonochemical process followed by the hydrothermal reaction at different temperatures and times. CdS (hcp) crystals composing of Cd and S were detected using XRD, SAED and EDX. A Raman spectrometer revealed the presence of fundamental and overtone modes at 299 and 596 cm(-1), respectively. The crystals are nanorods growing in systematic array analyzed using TEM and HRTEM. Their photoluminescence is in the blue spectral region at 409-422 nm (2.94-3.03 eV). Key words: CdS; Nanorods; Sonochemical and hydrothermal processes CHALCOGENIDE LETTERS. Volume: 7. Issue: 9. Pages: 552-558. Published: SEP 2010. 407 Utilization of ceramic waste as fine aggregate within Portland cement and fly ash concretes Torkittikul Pincha and Chaipanich Arnon Construct Materials Research Unit, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The aim of this research work was to investigate the feasibility of using ceramic waste and fly ash to produce mortar and concrete. Ceramic waste fragments obtained from local industry were crushed and sieved to produce fine aggregates. The measured concrete properties demonstrate that while workability was reduced with increasing ceramic waste content for Portland cement concrete and fly ash concrete, the workability of the fly ash concrete with 100% ceramic waste as fine aggregate remained sufficient, in contrast to the Portland cement control concrete with 100% ceramic waste where close to zero slump was measured. The compressive strength of ceramic waste concrete was found to increase with ceramic waste content and was optimum at 50% for the control concrete, dropping when the ceramic waste content was increased beyond 50%. This was a direct consequence of having a less workable concrete. However, the compressive strength in the fly ash concrete increased with increasing ceramic waste content up to 100%. The benefits of using ceramic waste as fine aggregate in concrete containing fly ash were therefore verified. (C) 2010 Elsevier Ltd. All rights reserved. Key words: Ceramic waste; Fly ash; Fine aggregate replacement; Compressive strength CEMENT & CONCRETE COMPOSITES. Volume: 32. Issue: 6. Pages: 440-449. DOI: 10.1016/j.cemconcomp.2010.02.004. Published: JUL 2010. 408 Utilization of fly ash with silica fume and properties of Portland cement-fly ash-silica fume concrete Nochaiya Thanongsak, Wongkeo Watcharapong and Chaipanich Arnon Construct Materials Research Unit, Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract This paper reports the normal consistency, setting time, workability and compressive strength results of Portland cement-fly ash-silica fume systems. The results show that water requirement for normal consistency was found to increase with increasing SF content while a decrease in initial setting time was found. Workability, measured in term of slump, was found to decrease with silica fume content (compared to blends without silica fume). However, it must be noted that despite the reduction in the slump values, the workability of Portland cement-fly ash-silica fume concrete in most cases remained higher than that of the Portland cement control concrete. Furthermore, the utilization of silica fume with fly ash was found to increase the compressive strength of concrete at early ages (pre 28 days) up to 145% with the highest strength obtained when silica fume was used at 10 wt%. Moreover, scanning electron micrographs show that utilization of fly ash with silica fume resulted in a much denser microstructure, thereby leading to an increase in compressive strength. (C) 2009 Elsevier Ltd. All rights reserved. Key Words: HIGH-STRENGTH CONCRETE; COMPRESSIVE STRENGTH; MINERAL ADMIXTURES; ACTIVATION FUEL. Volume: 89. Issue: 3. Pages: 768-774. DOI: 10.1016/j.fuel.2009.10.003. Published: MAR 2010. 409 Weak and strong convergence theorems for a finite family of generalized asymptotically quasi-nonexpansive mappings Cholamjiak Watcharaporn and Suantai Suthep 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 PERDO National Center for Excellence in Mathematics, Mahidol University, Bangkok 10400, Thailand. Abstract In this paper, we introduce a new iterative scheme for finding a common Fixed point of a finite family of generalized asymptotically quasi-nonexpansive mappings in a uniformly convex Banach space. We establish weak and strong convergence theorems. Our main results improve and extend the corresponding ones obtained in Schu (1991) [J. Schu, Iterative construction of fixed points of asymptotically nonexpansive mapping, J. Math. Anal. Appl. 159 (1991) 407-413] and many others. (C) 2010 Elsevier Ltd. All rights reserved. Key words: Generalized asymptotically quasi-nonexpansive mapping; Iterative method; Common fixed point; Banach space; Strong convergence COMPUTERS & MATHEMATICS WITH APPLICATIONS. Volume: 60. Issue: 7. Pages: 1917-1923. DOI: 10.1016/j.camwa.2010.07.025. Published: OCT 2010. 410 Weak Convergence Theorems for a Countable Family of Strict Pseudocontractions in Banach Spaces Cholamjiak Prasit1 and Suantai Suthep1,2 1 Department of Mathematics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Higher Education, Center of Excellence in Mathematics, Bangkok 10400, Thailand. Abstract We investigate the convergence of Mann-type iterative scheme for a countable family of strict pseudocontractions in a uniformly convex Banach space with the Frechet differentiable norm. Our results improve and extend the results obtained by Marino-Xu, Zhou, Osilike-Udomene, Zhang-Guo and the corresponding results. We also point out that the condition given by Chidume-Shahzad (2010) is not satisfied in a real Hilbert space. We show that their results still are true under a new condition. Key Words: COMMON FIXED-POINTS; NONEXPANSIVE-MAPPINGS; PSEUDOCONTRACTIONS; LIPSCHITZ PSEUDOCONTRACTIONS; ITERATION PROCESS; HILBERT-SPACES; FINITE FAMILY; CONSTRUCTION FIXED POINT THEORY AND APPLICATIONS. Article Number: 632137. DOI: 10.1155/ 2010/632137. Published: 2010. 411 Webcam camera as a detector for a simple lab-on-chip time based approach Wongwilai Wasin1,2,3, Lapanantnoppakhun Somchai1,2,3, Grudpan Supara4 and Grudpan Kate1,2,3 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Research Laboratory for Development of Analytical Instrumentation, Center for Instrument & Process, Science & Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 4 College of Art Media & Technology, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract A modification of a webcam camera for use as a small and low cost detector was demonstrated with a simple lab-on-chip reactor. Real time continuous monitoring of the reaction zone could be done. Acid-base neutralization with phenolphthalein indicator was used as a model reaction. The fading of pink color of the indicator when the acidic solution diffused into the basic solution zone was recorded as the change of red, blue and green colors (%RBG.) The change was related to acid concentration. A low cost portable semi-automation analysis system was achieved. (c) 2010 Elsevier B.V. All rights reserved. Key Words: CCD-CAMERA; PROTEIN; BINDING TALANTA. Volume: 81. Issue: 3. Pages: 1137-1141. DOI: 10.1016/j.talanta.2010.01.058. Published: MAY 15 2010. 412 yclic microwave assisted synthesis of Sb(2)S(3) dumb-bells using polyvinylpyrrolidone as a template and splitting agent Kavinchan Jutarat1, Thongtem Titipun1 and Thongtem Somchai2 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Antimony sulfide (Sb(2)S(3)) was successfully synthesized from antimony chloride (SbCl(3)) and sodiumthiosulfate (Na(2)S(2)O(3)) in ethylene glycol (EG) containing different polyvinylpyrrolidone (PVP) masses under cyclic microwave radiation. XRD and SEM show that the products were orthorhombic Sb(2)S(3) nanorods forming the dumb-bells. TEM study reveals Sb(2)S(3) single-crystal nanorods, growing along the [001] direction. The dumb-bell formation is also proposed, according to the splitting ends and a middle tie-up. Its direct band gap, calculated from UV-visible absorption, is 1.52 eV. (C) 2010 Elsevier B.V. All rights reserved. Key Words: ROUTE MATERIALS LETTERS. Volume: 64. Issue: 21. Pages: 2388-2391. DOI: 10.1016/j.matlet.2010.07.075. Published: NOV 15 2010. 413 ZnO Nanobelts as a Photoelectrode for Dye-Sensitized Solar Cell Hongsith Niyom and Choopun Supab 1 Department of Physics & Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Commission on Highter Education, Thailand Center of Excellence in Physics, Bangkok 10400, Thailand. Abstract ZnO nanobelts layer of the dye-sensitized solar cell were prepared by RF sputtered ZnO target onto a copper substrate and characterized by FE-SEM. The structures of solar cells based on ZnO as a photoelectrode, Eosin-Y as dye sensitizer, iodine/iodide solution as an electrolyte and Pt/TCO as a counterelectrode. The photoelectrochemical characteristics of ZnO DSSCs were tested under simulated sulight AM 1.5 came from a solar simulator with the radiant power of 100 mW/ cm(2). It was found that DSSCs based on ZnO nanobelts can generate photocurrent with photoconversion efficiency of 0.6% (J(SC) = 2.11 mA cm(2), V(OC) = 0.52, FF = 0.61). The higher J(SC) in ZnO nanobelt DSSC sample indicates that larger amount of dye adsorbed on surface of ZnO nanobelts than that of ZnO powder. The J(SC) increases with increasing a thickness of the ZnO photoelectrode for both powder and nanobelt. Moreover, the of ZnO DSSCs is independent on morphology and dye adsorption surface area of ZnO. The obtained photoelectrochemical results can be explained by using energy band diagram. Key Words: FILMS; ELECTRODES CHIANG MAI JOURNAL OF SCIENCE. Volume: 37. Issue: 1. Pages: 48-54. Published: JAN 2010. 414 Artwork and Community History around Burmese-Tai Yai Temples in Lampang Suebsak Sanyakiatikun1, Supaporn Nakbunlung2, Usanee Thongchai3, Chatkaew Simarak3, Sineenad Somboonanek4, Woralun Boonyasurat1, Thianchai Aksrondit1, Anukoon Siripan6, Metee Medhasith Suksumret5, Thapakron Kruaraya5, Nuttapon Sukitsuksawas5, Thapanee Kruaraya5, Medapon Bomkao5 and Jutamas Suvimolcharoen5 1 Faculty of Fine Arts, Chiang Mai University, Chiang Mai 50200, Thailand. Faculty of Social Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Faculty of Humanities, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Chiang Mai University Library, Chiang Mai University, Chiang Mai 50200, Thailand. 5 Northern Archaeology Center, Chiang Mai University, Chiang Mai 50200, Thailand. 6 Lampang Inter-Tech College, Lampang 52100, Thailand. 2 ABSTRACT The study of 15 Burmese-Tai Yai Temples in Lampang reveals that most of them were built by Burmese-Tai Yai merchants who had business with Western company during 25th BE. The Art Style of the temples reflected Burmese influence, with their belief system embedded on the designs, inherited by the artisans. As they are not community temples, and the conflict between some temples and their communities about the land lead to the decaying of the temples. This study also assisted the community to understand about the important of the religious monument and to realize about the need to participate in the temple activities. During the process of field data collecting, young researchers has been trained in terms of conservation and development. Published in Artwork and Community History around Burmese-Tai Yai Temples in Lampang Research, Northern Archaeology Center, 2010. 415 Prehistoric Culture of Pra Tu Pha Limestone Hills in Mae Moh District, Lampang Province Supaporn Nakbunlung1, Valery Zeitoun2, Piyawit Moonkham3, Nuttapon Sukitsuksawas3 and Jutamas Suvimolcharoen3 1 Faculty of Social Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Institut de Recherche pour le Développement, French. 3 Northern Archaeology Center, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT The archaeological site of Ban Tha Si is located 10 km south of the famous painting rock art site of Pra Tu Pha (Mae Mo District, Lampang Province). The excavation was undertaken to understand the use of rock-shelter during the prehistoric time and to bring knowledge of the very old period of Thai heritage.The rock-shelter of Ban Tha Si is situated at the western wall of Doi Pha Kan. Several red painted figures of human hands and also figures of animal (an elephant and a sun bear) are present. After the mapping of the topography of the site we collect 48 stone tools, 5 sherds and one cowrie during the surface survey of the site. A first trench of 5 meters long and 1 meter wide excavated down to the elephant painting provided only 4 stone tools and show that the soil is very eroded. Nevertheless, a second test-pit undertaken under the eastern arch of the rock-shelter provided 10 pebbles, 202 flakes, 20 chunks, and 54 stone tools (scrapper, denticulated and retouched flakes) made of silicified limestone and 85 sherds (less than 1 cm wide) of a black ceramic. Small remains of animals show the presence of turtle, varan, snake, crab, cowrie, suid, cercopithecid and large bovid showing that human live at that place for eating after hunting. But other activities are present. Indeed, the burial of an adult male provide half body of an individual. His body is laying on his right side. His head is not present anymore but the arms are flexed with the hands close to the chin. His legs are also in flexed position. The stature of the individual is around 1.73 meters tall. The dating of the charcoal suggests a human occupation of this place between 12,000 and 6,000 years ago. The burial belongs to the youngest period. The prehistoric site of Ban Tha Si is very similar to the famous Moh Khiew site from southern Thailand and suggests that human have the same way of life from southern to northern Thailand at prehistoric times. Published in Prehistoric Culture of Pra Tu Pha Limestone Hills in Mae Moh District, Lampang Province Research, Northern Archaeology Center, 2010. 416 BrimA, total Acidity and total soluble solid correlate to total carotenoid contents as indicators of the ripening process of six Thai mango fruit cultivars Anyarat Wongkhot1,2, Nithiya Rattanapanone1,2,3 and Usawadee Chanasut1,2,4 1 Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 3 Food Science and Technology Department, Agro-Industry Faculty, Chiang Mai University, Chiang Mai 50200, Thailand. 4 Biology Department, Science Faculty, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT The investigated was carried out to correlate BrimA values with total carotenoid content in comparison to TSS/TA ratios and total acidity (TA) with total carotenoid content of six mango (Mangifera indica L.) cultivars. The cultivars of these mangoes were “Kaew”, Khew Sawoey”, “Chok-Anan”, “Nam Dok Mai”, Maja-Chanok” and “Nang Klangwan”. Mature green mango fruits were selected based on their specific gravity which was greater than 1.0 and then kept at 25±2ºC with 70-80 % RH. The sampling was carried out randomly on daily basis until mango fruits ripened. The result showed that BrimA value, TSS/TA ratios with total carotenoids content (TCC) of all cultivars increased duting ripening process. The subsequent determination of correlation coefficient (r2) suggested that the TCC were highly related to BrimA values rather that the TSS/TA ratios and TA, in some cultivars. The best corresponding r2 values of BrimA and total carotenoids content were 0.88 and 0.99 for cv. “Kaew” and “Nang Klangwan”, respectively. Therefore, correlation between BrimA and TCC may possibly be used as an index for the ripening stage, flesh color and sweetness of mango fruit. Published in Chiang Mai University Journal of Natural Science, Volume 10 Number 1 (January-June 2011). 417 Comparative Characterisation of Durian, Mango and Avocado Sumitra Poovarodom1, Ratiporn Haruenkit2, Suchada Vearasilp3, Jacek Namiesnik4, Milena Cvikrova5, Olga Martincova5, Aviva Ezra6, Milan Suhaj7, Pramoj Ruamsuke8 and Shela Gorinstein6 1 Department of Soil Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand. 2 Faculty of Agro-Industry, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand. 3 Postharvest Technology Research Institute, Chiang Mai University 50200, Thailand. 4 Department of Analytical Chemistry, Chemical Faculty, Gdansk University of Technology, 80 952 Gdansk, Poland. 5 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic. 6 School of Pharmacy, Institute for Drug Research, The Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel. 7 Food Research Institute, 824 75 Bratislava, Slovakia. 8 Faculty of Agricultural Technology, Rambhai Barni Rajabhat University, Chanthaburi 220000, Thailand. ABSTRACT The aim of this investigation was to determine the nutritional and bioactive properties of relatively less investigated exotic fruit durian (Durio zibethinus Murr.) and to compare these indices with widely used mango (Mangifera indica L.) and avocado (Persea Americana). For this purpose HPLC, three-dimensional fluorescence (3D-FL), several radical scavenging assays and multivariate factor analysis were used. It was found a similarity in acetone extracts between durian and mango in contents of polyphenols (1.66±0.08, 1.48±0.05, mg GAEg-1 DW, respectively), and in some antioxidant assays such as ABTS (11.98±0.5, 12.24±0.5, µM TEg-1DW, respectively and DPPH (5.61±0.3, 5.22±0.2, µM TEg-1DW, respectively). Durian and avocado were similar in the contents of polyphenols, and ABTS and DPPH values in water and in methanol extracts, respectively. Base on the obtained results the nutritional and bioactive properties of durian are comparable with those indices in mango and avocado. In conclusion, durian can be recommended as a part of disease prevented diets. Published in International Journal of Food Science and Technology 45, 921-929. 418 Diversity of Moulds in Fresh Longan Piyanat Chang-ngern1,2,3, Uraporn Sardsud1,2,3, Wasu Pathom-aree1, Parinya Chantrasri4 and Ekachai Chukeatirote5 1 Biology Department, Science Faculty, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 4 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 5 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. 2 ABSTRACT The objective of this study was to determine the diversity of moulds on harvested fresh longan for dried product preparation. Initially, fresh longan were collected from Chiang Mai in July 2007; the fungi were then isolated from pericarp, fruit, seed and stem-end by tissue transplanting technique and mould identification was performed based on morphological characteristics. A total of 121 isolates were obtained in which 83 isolates (68.59%) were from pericarp; 32 (26.45%) from fruit; and 6 (4.96%) from seed and stem-end, respectively. The mycobiota was dominated by Lasiodiplodia (30.60%), Aspergillus (14.10%), Penicillium (11.00%), Xylaria (14.10%), Fusarium (5.00%), Rhizopus (4.5%), Pestalotiopsis (4.5%) and Trichoderma (3.50%). Besides, unidentified fungi were also present and accounted for 11.60%. Published in Agricultural Science Journal. 41 (1) (Suppl.): 322-324. 419 Drying Kinetics and Dried Product Quality of Rosemary Leaves, Lavender Flowers and Rose Petals Pichaya Boonprasom Poonlarp1,2,3, Autcharaphorn Apiwongngam1 and Yongyuth Chalearmchart1,2,3 1 Food Engineering Department, Agro-Industry Faculty, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. ABSTRACT The research was aimed at studying the drying kinetics of rosemary leaves, lavender flowers and rose petals. Three drying kinetics model were employed, namely Lewis, Henderson & Pabis, and Page Model. Rosemary leaves, lavender flowers and rose petals were dried at temperatures of 50°C with air velocities of 0.5 m/s using tray dryer. Initial moisture content of rosemary leaves, lavender flowers and rose petals were around 316.67%, 354.55% and 455.56% dry basis, respectively. The Page model was found to be the best model for describing the characteristics of rosemary leaves, lavender flowers and rose petals for drying at the temperatures of 50°C. Color assessment, moisture content, water activity (aw) total ash, tannin, total phenolic compounds, total plate count, yeastmold, coliform and E. coli were analyzed as the quality parameters. Dried product qualities of rosemary leaves, lavender flowers and rose petals using solar dryer with moisture content of less than 7% were comparable to those dried in tray dryer. In addition, the dried products from three different dryers had the moisture content less than 7%. Published in Agricultural Science Journal. 41 (1) (Suppl.). 420 Effect of electrolyzed oxidizing water and continuous ozone exposure on the control of penicillium digitatum on tangerine cv. ‘Sai Nam Pung’ during storage Kanda Whangchai1,2,3, Kobkiat Saengnil1, Chananchida Singkamanee2,3 and Jamnong Uthaibutra1,2,3 1 Biology Department, Science Faculty, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 2 ABSTRACT The effect of electrolyzed oxidizing (EO) water in combination with ozone to control postharvest decay of tangerine cv. “Sai Num Pung” was investigated. The spore suspension containing 105 conidia ml-1 of Penicillium digitatum was prepared. EO water was generated by electrolysis of various concentrations of NaCl solution (5, 25, 50% and saturated NaCl). The spore suspension was inoculated into EO water and incubated at 27°C for 1, 2, 4, 8 and 32 min. It was found that the EO water with saturated NaCl completely inhibited the spore germination of the fungus within 1 min. When the fruit inoculated with P. digitatum were washed in EO water at the same concentration as previous experiment for 4, 8 and 16 min and stored at 5°C for 18 days, it was found that immersion of the fruit in EO water for 8 min was the most effective to reduce disease incidences. Moreover, washing fruit in EO water and kept in a refrigerated chamber at 5°C with continuous zone exposure at a concentration of 200 mgl-1 for 2 h day-1 to extend storage life suppressed the disease incidence until 28 days. However, none of the treatments had any effect on the quality of fruit such as total soluble solids, titratable acidity, percent weight loss and peel color. Therefore EO water may be useful for surface sanitation and ozone has potential to control the recontamination of postharvest diseases in tangerine fruit in storage room. Published in Crop Protection 29: 386-389. 421 Effect of Hydrogen Peroxide on the Quality of Abalone Mushroom Thanyaporn Boonphokaew1,2,3 and Uraporn Sardsud1,2,3 1 Biology Department, Science Faculty, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 2 ABSTRACT Abalone mushroom is one of the favourite edible mushrooms due to its firm texture and characteristic flavor. However, fresh mushroom has short shelf life because it quickly loss of quality after harvest. So, it decays easily and its market could not be expanded fully. The mean of extending shelf life has to be looked for. At present, the addition of food preservative is rather limited for the safety of consumers. Hydrogen peroxide is regarded as Generally Recognized As Safe (GRAS) and is added to some food and it is generally acceptable as safe for consumption. The objective of this research was to study the effect of hydrogen peroxide on the quality of abalone mushroom. The mushroom was sliced lengthwise, 0.5 cm thick and dipped in hydrogen peroxide at the concentrations of 0, 5, 10, 15, 20, 25, 30 and 35% respectively, each concentration for 0, 10, 20, 30, 40, 50 and 60 minutes and kept at 10°C. The changes in color and firmness were determined every 4 days. It was found that the shelf life of mushroom dipped in 5 and 10% hydrogen peroxide for 10 minutes was extended up to 16 days. Whereas those dipped in water and the non-dipped mushroom could be kept for only 12 days This research may provide a mean of extending shelf life of abalone mushroom and increase the possibility of expanding the mushroom market. Published in Agricultural Science Journal. 41 (1) (Suppl): 122-125. 422 Effect of Radio Frequency Heat Treatment to Control Aspergillus flavus in Maize (Zea mays) Kulthida Chaisathidvanich1,2, Wolfgang Lücke3, Nattasak Krittigamas1,2,5, Sa-Nguangsak Thanapornpoonpong1,2,4, Sangtiwa Suriyong4 and Suchada Vearasilp1,2,4 1 Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 3 Institute of Agricultural Engineering, University of Göttingen, Germany. 4 Plant Science and Natural Resources Department, Agriculture Faculty, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT The use of radio frequency (RF) heat treatment to eliminate Aspergillus flavus in maize seeds was investigated in this study. The spore suspension of A. flavus isolated from infected maize seeds was inoculated in the maize seed lot. The inoculated seed samples were treated with an RF application of (27.12 MHz) at 80, 85 and 90°C for both 1 min and 3 min. The percentage of fungal infection detected by the blotter and Potato Dextrose agar (PDA) methods.The percentage of moisture content, grain cracking and aflatoxin contamination was also determined and evaluated. The results showed that using heat treatments of more than 85 and 90°C, at duration times of 1 minute and 3 minutes significantly affected the seed moisture content. Seed moisture was decreased significantly by more than 0.50 percent. The percentage of cracking was increased by 33% and 38% when the maize grains were treated with 90°C for 1 min and 3 min, respectively. Radio frequency heat treatment, at the temperature of 90°C for 3 minutes, completely eliminated A. flavus when determined by the blotter method, whereas using the PDA method, the fungi remained contaminated at about 1%. In addition, aflatoxin contamination was not detected from all treated maize samples. Therefore, RF heat treatment at the temperature of 90°C for 3 min is suggested for eliminating A. flavus in maize seed. Published in Agricultural Science Journal. 41 (1) (Suppl.): 341-344. 423 Inhibitory Effect of Titanium Dioxide (TiO2) Photocatalytic Ozonation on Controlling Anthracnose Disease of Bird Chilli (Capsicum fruescens L.) After Harvest Pattraporn Chutidumrong1,2,3, Kanda Whangchai1,2,3, Satit Phiyanalinmat3 and Jamnong Uthaibutra1,2,3 1 Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 3 Biology Department, Science Faculty, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT The effect of titanium dioxide photocatalysis and ozone on growth inhibition of Colletotrichum capsici causing anthracnose disease of bird chilli was studied. The mixture of 2.6 x 106 spores/milliliter spore suspension of C. capsici and 10 milligram/milliliter of titanium dioxide powder were added and then exposed to ozone at the concentration of 200 ppm. After that, the samples were analyzed for fungal development (percent of mycelial inhibition and spore germination) every 10 minutes for 1 hour. The results showed that the titanium dioxide photocatalytic ozonation and ozonation alone were the most effective to inhibit fungal development. For the second experiment, bird chilli with artificial inoculation were washed in titanium dioxide photocatalysis-containing water combined with ozone for 25, 50, 75 and 100 minutes. The results showed that all treatments had effect on growth inhibition of fungi with 25 minutes, when compared with the control (distilled water). Published in the Proceeding on the 9th Horticultural Congress 2010/11-14 May 2010. 424 PCR-based Detection and Differentiation of Anthracnose Pathogen isolates from ‘Nam Dok Mai Si Thong’ Mang Orchards at Prao, Chiang Mai Parinya Chantrasri1,2,3 and Pongsathorn Dhumtanom1 1 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 2 ABSTRACT The trials was conducted in ‘Nam Dok Mai Si Thong’ mango orchards at Prao district, Chiang Mai Province, from October 2009 to February 2010 with 5 year-old trees of the mango to study the quiescent of anthracnose disease of mango caused by Colletotrichum gloeosporioides form pruning stage to flowering stage. More than 2 species of the fungal pathogens were isolated from leaf and branch samples by using paraquat technique to induce the growth of the fungi. Polymerase chain reaction technique was used for detection of the plant pathogen. For early detection of anthracnose pathogen of mango, two sets of oligonucleotide primers, ITS1-4 and ITS4-5, specific to Genus Colletotrichum were used. PCR products were subsequently sequencing and at least 2 isolates of Colletotrichum spp. were confirmed. Species-specific primers pairs will design for further detection of Colletotrichum gloeosporioides in the mango orchards. Published in the Proceeding on the 9th Horticultural Congress 2010/11-14 May 2010. 425 The Decrease of Quiescent and Anthracnose Disease for Increasing Export Potential of “Nam Dok Mai Si Thong” mango at Prao in Chiang Mai Parinya Chantrasri1,2,3, Vicha Sardsud2,3, Uraporn Sardsud2,3 and Rattapol Pornprasit2,3 1 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 2 ABSTRACT Assessment of the quiescent of Colletotrichum gloeosporioides causing anthracnose disease of mango and its control was conducted in Prao district of Chiang Mai Province, from October 2008 to April 2009 with 4-5 year-old trees of the mango variety “Nam Dok Mai Si Thong”. The effects of pre-harvest spray program every two week interval of copper oxychloride, mancozeb, carbendazim, azoxystrobin and prochloraz on post-harvest development of anthracnose disease was also studied. Eight pre-harvest applications of the fungicides from prunin to bagging stage could control the disease on fruit harvested 110 days after floral induction and incubated at ambient temperatures for 14 days. Chemical sprayed trial farm show less disease than the nearby orchard where only azoxystrobin and mancozeb was applied. Data from in vitro test by using paraquat stimulating technique showed that the management of pre-harvest applications had significantly reduced the number of anthracnose pathogen compared with those samples recovered from control trees. Dipping mangoes in prochloraz and hot water (at 55°C for 5 minutes) reduced decay caused by the anthracnose pathogen to levels similar to those attained by using hot prochloraz. When fruits were stored for longer periods of time, prochloraz was more effective than the hot water treatment alone. Published in Agricultural Science Journal. 41 (1) (Suppl.): 307-310. 426 The Abstracts Presented in the conferences CORRELATION OF DELIVERY MODES AND EARLY CHILDHOOD CARIES Sakornrat Khongkhunthian1, Prakaimuk Saraithong2, Komkham Pattanaporn3, YiHong Li4, Pannipa Laohapensang5 and Wisut Pattanaporn5 1 Department of Restorative Dentistry and Periodontology, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 University of British Columbia, Vancouver, Canada. 4 The college of Dentistry, New York University, New York, USA. 5 Health Promotion Hospital, Chiang Mai, Thailand. ABSTRACT The mode of delivery and other maternal factors contribute to the acquisition of S. mutans in infants, which may lead to differences in caries experience in later years. Objectives: To determine the association between the mode of delivery and other maternal factors with the colonization of S. mutans and caries experience in a group of Thai children. Methods: A total of 350 mothers and their 3- or 5-year-old children were randomly selected from the Health Promotion Hospital in Chiang Mai, Thailand; 182 were born vaginally and 168 were born by caesarean section. Caries experiences of the mothers and the children were examined. S. mutans colonization was assessed by using Dentocult® SM Strip Mutans method (Orion Diagnostica) and quantitative real-time PCR. Information on childcare history, dietary, and oral health practices were obtained by a questionnaire survey of the mothers. Results: A proportion of children with very high S. mutans colonization (Strip mutans score = 3) was found in 3-year-old (45.4%) vaginally-born children (P = 0.017). Three-year-old vaginally born children also experienced more caries (64.9%) compared with age-matched caesarean born children (45.9%) (P <0.001) with higher mean dmfs score (6.8±10.3 vs. 4.3±7.6; P = 0.023). A significant association was found between S. mutans colonization and caries prevalence for both 3- and 5-year-old groups (P <0.001). Other maternal factors associated with more caries in the children included chewing food to feed their child (P = 0.023), bottle feeding frequency at bed time (P = 0.015), soft-drink consumption (P = 0.04), and use of fluoride toothpaste (P = 0.021). Findings from qPCR also confirmed that vaginally delivered children had higher prevalence of S. mutans 429 than that C-sectionally delivered children. The difference was highly significant for the 3-year-old group and was diminished by the age of 5, the delivery mode had no effect on the colonization of S. sobrinus in the children. There were correlation of S. mutans and S. sobrinus colonization and dental caries in children. Mothers’ S. mutans and S. sobrinus colonization did show effects on children’s mean dmft scores. There was no significant correlation of S. mutans and S. sobrinus colonization between the mothers and the children from both qualitative, melting curve, and quantitative, Ct value and mean DNA levels, determinations were based on qPCR results. Conclusion: This preliminary study demonstrates that maternal mode of delivery and feeding practices were significantly associated with S. mutans colonization and caries status in a population of Thai children. Presented in the 9th World Congress on Preventive Dentistry, Phuket, Thailand, September 7-10, 2009 and The AADR Annual Meeting & exhibition, Washington. DC, USA March 3-6, 2010. Supported by Faculty of Dentistry, Chiang Mai University, The college of Dentistry of New York University and Colgate-Palmolive Company, USA. 430 CRUDE MALVA NUT GUM AFFECTS PASTING AND TEXTURAL PROPERTIES OF WHEAT FLOUR IN THE PRESENCE OR ABSENCE OF SODIUM CHLORIDE Yuthana Phimolsiripol1, Ubonrat Siripatrawan2 and C. Jeya K. Henry3 1 Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. 2 Department of Food Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. 3 School of Life Sciences, Oxford Brookes University, Oxford, UK. ABSTRACT Malva nut seed contains a large amount of mucilaginous substance. However, information of the application of crude malva nut gum (CMG) in starch-based food system is limited. Understanding of the interaction phenomena between the mixtures will be useful for the development of starch-based products with better quality. This research aims to investigate the effect of CMG addition on pasting and textural properties of wheat flour in the presence or absence of sodium chloride (NaCl). CMG was extracted by soaking in water and then precipitating with 95% ethanol. The mixtures were prepared by adding 0, 2.5, 5, 7.5 and 10% of CMG into wheat flour. Pasting results revealed that addition of CMG significantly elevated (p<0.05) the peak viscosity (128-669 RVU), hot paste viscosity (77-363 RVU), breakdown (50306 RVU) and final viscosity (157-556 RVU). Pasting temperature (59-85oC) of the pastes decreased with increasing CMG content. The textural parameters including hardness, springiness, cohesiveness, gumminess and chewiness of the mix gels decreased with the addition of CMG. The presence of 0.75% NaCl in the mixtures had higher viscosity than the presence of 0 and 1.5% NaCl in the mixtures. The hardness of gel mixed with 0-2.5% CMG increased with increasing NaCl levels, while no significant effect (p≥0.05) at higher level of CMG (7.5-10%). Higher paste viscosity, softer gel texture of wheat flour could be altered using CMG and the presence of NaCl affects the pasting and textural properties. Presented in International Conference on Agriculture and Agro-Industry (ICAAI2010): Food, Health and Trade. Chaing Rai, Thailand, November 2010. 431 DEVELOPMENT OF COCONUT MILK ICE CREAM MIXED WITH MALVA NUT JELLY Panjarus Wongyai, Supansa Dudngon and Yuthana Phimolsiripol Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. ABSTRACT The objective of this research was to apply malva nut jelly (MJ) in coconut milk ice cream. Mixture experiment was designed to investigate the ratio of coconut milk, skim milk and malva nut jelly. Results showed that optimal ratio was 44.4% coconut milk, 5% skim milk and 5% MJ. In addition, increasing MJ content reduced sweetness of ice cream. Then, CRD experiment was done at 5, 15, 25 and 35% MJ based on the total mixture. Total soluble solid of ice cream was controlled at 25oBrix. Use of 25% MJ and 21% sugar resulted in a high hedonic score. However, higher viscosity and less sweetness were recommended by consumers. Factorial experiment was used to study sucrose and stabilizer (mixtures of xanthan gum and guar gum at 1:3) contents. It was found that use of 21% sugar and 0.2% stabilizer in ice cream had the highest overall-liking score. Optimum formula of developed product consisted of 34.6% coconut milk, 20.2% sugar, 19.2% water, 18.3% MJ, 3.8% skim milk, 1.9% stabilizer, 1% carboxymethylcellulose and 1% glyceryl monostearate. Hundred percent of consumers accepted the developed product. Presented in the 2nd Agro-Industry Chiang Mai University Conference. Chiang Mai Thailand, November 2010. 432 DEVELOPMENT OF GLUTEN-FREE BREAD MIXED WITH JIAOGULAN Krongkarn Pugpayappysal, Pornthiva Takwang and Yuthana Phimolsiripol Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. ABSTRACT The objective of the research was to develop gluten-free bread mixed Jiaogulan for providing higher phenolic content. Mixture experiment was designed to study type and ratio of appropriate hydrocolloids. Results showed that 1% of both guar gum and hydroxypropyl methylcellulose had significantly different effects (P<0.05) on the loaf specific volume. The effects of protein types (egg white powder and soy protein isolate) and dough proving processes (proving one time for 60 minutes and proving two times for 40 and 45 minutes) were studied using 2x2 factorial in CRD. It was found that use of egg white powder and dough proving for 60 minutes resulted in increasing loaf specific volume. The influences of water (100-150% of flour) and Jiaogulan powder (0-1% of flour) contents were investigated using CCD. Results demonstrated that the appropriate amount of water and Jiaogulan powder was 100% and 0.22%, respectively. Optimal formula of developed glutenfree bread mixed with Jiaogulan consisted of 45% rice flour, 45% corn flour and 10% soy flour. Other ingredients based on flour content included 100% water, 1.5% yeast, 1.75% salt, 10% sugar, 10% egg white powder, 10% shortening, 2% emulsifier, 0.22% Jiaogulan powder, 1% sodium bicarbonate, 1% guar gum, 1% hydroxypropyl methylcellulose and 1% Lecitex. The loaf specific volume and total phenolic compound of the developed bread were 2.42 mL/g and 0.675 mgGAE/g dry weight, respectively. Presented in The 2nd Agro-Industry Chiang Mai University Conference. Chiang Mai, Thailand, November 2010. 433 EFFECT OF CRUDE MALVA NUT GUM ON PASTING BEHAVIOR AND FREEZE-THAW STABILITY OF TAPIOCA STARCH Yuthana Phimolsiripol1, Suthat Surawang1 and Ubonrat Siripatrawan2 1 Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. 2 Department of Food Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. ABSTRACT This research is aimed to investigate the effect of CMG on pasting behavior and freeze-thaw stability of tapioca starch. CMG was extracted by soaking malva nut seed in water and then precipitating with 95% ethanol. Yield of CMG was about 19.4% based on dry weight of the seeds with 12.5% moisture content. Tapioca starch was replaced by different concentrations of CMG at 0, 0.5, 1, 2, 3 and 5% w/w. Different concentrations of CMG resulted in significant difference (p<0.05) in pasting behavior of tapioca starch. Peak viscosity and final viscosity significantly increased (p<0.05) with increasing in CMG concentrations which ranged from 362-815 RVU and 226-391 RVU, respectively. Setback from peak of tapioca starch replaced with 5% CMG decreased by about 2.23 times when compared to non-CMG sample. Freeze–thaw stability measurement revealed that the tapioca starch gel had no syneresis after freezing but syneresis percentage increased with increasing freeze-thaw cycles. Higher level of CMG in tapioca starch gel could decrease syneresis. When 3-5% CMG concentrations were used, syneresis of tapioca starch gel was lower than 3%. Therefore, CMG could be applied in frozen product to improve freeze-thaw stability. Presented in The 6th Chiang Mai University Academic Days: “Research Path: Innovation for Creative Economy and Sustainability”. Chiang Mai, Thailand, November 2010. 434 EFFECT OF SOAKING CONDITIONS ON PHYSICOCHEMICAL PROPERTIES OF RED-JASMINE GERMINATED BROWN RICE FLOUR Supanuch Saipang, Niramon Utama-ang and Yuthana Phimolsiripol Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. ABSTRACT The objective of this research was to study the effects of soaking temperature and soaking time on physicochemical properties of red-jasmine germinated brown rice flour. The experiment was conducted with the variation of soaking temperature (30 and 40°C) and soaking time (1, 3 and 6 hr). Results demonstrated that soaking conditions had no significantly effect (P≥0.05) on the chemical composition including moisture, protein, fat and ash content but resulted in increased crude fiber and decreased carbohydrate content from fermentation. The pH of soaking water decreased from 6.29 to 6.23-5.71 and affected to the change of pasting properties of germinated brown rice flour. Increase in soaking time resulted in lower pasting temperature of germinated flour than non-soaking flour and led to retrogradation reduction. Therefore, soaking rice with different conditions provided property modification of rice flour for further development of product from germinated brown rice. Presented in the 48th Kasetsart University Annual Conference. Bangkok, Thailand, February 2010. 435 PASTING BEHAVIOR AND FREEZE-THAW STABILITY OF WHEAT, RICE AND GLUTINOUS RICE FLOURS MIXED WITH MALVA NUT GUM SYSTEMS Yuthana Phimolsiripol1, Suthat Surawang1 and Ubonrat Siripatrawan2 1 Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. 2 Department of Food Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. ABSTRACT The objective of this research was to investigate the effect of crude malva nut gum (CMG) addition on pasting behavior and freeze-thaw stability of wheat, rice and glutinous rice flours. CMG was extracted by soaking in water and then precipitating with 95% ethanol. Pasting behavior and freeze-thaw stability of different flour mixtures with 0, 0.5, 1, 2, 3 and 5% of CMG were determined. Pasting temperature (60-87°C) of the pastes significantly decreased (p<0.05) with increasing CMG content for wheat and rice flours, while no significant effect (p≥0.05) for glutinous rice flour. Addition of CMG in all flours significantly elevated (p<0.05) the peak viscosity by about 0.9-2.6 folds when compared to non-CMG samples. Hot paste viscosity, breakdown and final viscosity for all flour mixtures significantly increased (p<0.05) with increasing of CMG which ranged from 81-427, 37-559 and 152-463 RVU, respectively. Freeze-thaw stability measurement showed that higher level of CMG in wheat and rice gel mixtures could decrease syneresis. However, CMG had no effect in retarding retrogradation of glutinous rice gel. Adding CMG into flours alters the pasting properties and syneresis effect. It is revealed that higher viscosity and lower syneresis of gels could be modified using CMG. Presented in the 3rd CMU-KU Joint Symposium, Chiang Mai, Thailand, August 2010. 436 QUALITY PARAMETERS AND GLYCEMIC INDEX OF GLUTEN-FREE COOKIE USING DIFFERENT SWEETENERS Suchada Nokthuan and Yuthana Phimolsiripol Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. ABSTRACT Special diets including low-glycemic index, low-calorie or gluten-free are increasingly interested by consumers in terms of mitigating many diseases or health problems. This study aimed to investigate the influence of three types of sweeteners (sucrose, maltitol and xylitol) on the quality parameters and glycemic index of gluten-free cookie made from rice flour. Sucrose in normal cookie formula was replaced by maltitol and xylitol as sweetness equivalence of sucrose concentration. Use of maltitol in cookie resulted in significantly higher (p<0.05) L* value than others, but no significant differences (p≥0.05) were observed in a* and b* values. Cookie with xylitol had significantly greater (p<0.05) moisture content and water activity than others which ranging between 2.87-3.68 and 0.2-0.3, respectively. In addition, cookie with xylitol had the lowest in hardness value. Replacing sucrose with maltitol and xylitol in cookie resulted in lower glycemic index. Sensory evaluation revealed that mean hedonic scores of cookie with maltitol were not significant differences (p≥0.05) in aroma, taste, hardness and overall-liking from cookie with sucrose. Replacing sucrose with maltitol in gluten-free cookie had similar physical properties to cookie with sucrose but it is more nutritious by lower glycemic index. Presented in the 8th Kaset Naresuan Conference. Phitsanulok, Thailand, August 2010. 437 A Novel Neuro-Fuzzy Method for Linguistic Feature Selectionand Rule-Based Classification Narissara Eiamkanitchat1, Nipon Theera-Umpon1,2 and Sansanee Auephanwiriyakul3 1 Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Biomedical Engineering Center, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Computer Engineering, and Biomedical Engineering Center, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This paper proposes a new interpretable neuro-fuzzy classification mechanism. The proposed neuro-fuzzy structure is different from other data analysis mechanisms previously invented in pattern recognition. General mechanisms focus mainly on creating predictive data models whereas some useful information inside the process may be ignored. The proposed mechanism is designed based on the consideration of feature selection and rule extraction. It is a three-layer feedforward network. Its structure can be comprehended to logical rules using only selected important features. We construct a new classification algorithm by using a small number of features that represent an informative subset of a given dataset. This classifier can produce good classification results from the direct calculation or from logical rule extraction. Pleasant performance of classification results are acquired from 10-fold cross validation testing on several standard datasets. Presented at International Conference on Computer and Automation Engineering, Oct. 27-29, 2010. 438 Application of Cleaner Technology and Experimental Design for the Reduction of Chemical Substance Consumption in the Hard Disk Drive Arm Coil Assembly Process Premchai Moolla and Rungchat Chompu-inwai Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The objective of this research is to apply the Cleaner Technology (CT) and Design of Experiment (DOE) concepts in the hard disk drive arm coil assembly process, in order to reduce chemical substance (i.e. epoxy and Isopropyl Alcohol (IPA)) consumption. In the production process, epoxy is used to fix the arms and coils, whereas IPA is used to clean the arms before they are delivered to the production process. These chemicals are toxic and cause irritation to workers if they are not careful. Furthermore, the ineffective use of these chemicals adversely affects production costs. The research methodology was divided into two main parts. The first part employed the CT concept to analyze the loss during production, as well as the causes of loss. Opportunities to reduce those losses could then be recommended. The next step was to further analyze and evaluate a particular CT opportunity using DOE. In this second part of the research, DOE was used to reduce the chemicals used in the process by finding the optimum amount of chemicals used which does not affect the quality of the product. Full factorial experiment 2k, with two replicates was used for screening the four factors, these being: IPA, work-piece curing, epoxy usage and air pressure. The response variable was the arm coil bonding force. The analysis of variance (ANOVA) was conducted. The results of the study show that the optimal operating conditions are: 1) to clean the arms without using IPA, 2) to cure the work-pieces, 3) the filling of 65 mg of epoxy between the arm and the coil, and that 4) there is no significant impact of the air pressure on the arm coil bonding force. The results of the confirmation test show that the arm coil bonding force is in accordance with customer specifications. The results of the experiment have subsequently been put into practice. Thus, reducing the amount of epoxy wasted in the production process helps save approximately 70,208 Baht per annum, while cleaning without using IPA can save 2,154,240 Baht per annum. In conclusion, applying CT and DOE can help to make overall cost savings of 2,224,448 Baht per annum. Presented at the 40th International Conference on Computers and Industrial Engineering 2010, July 25-28, 2010. 439 Application of Cleaner Technology Concepts in the Arm Coil Assembly Process of Hard Disk Drive Manufacturing Rungchat Chompu-inwai and Premchai Moolla Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT The objectives of this research are to improve raw material and energy consumption efficiency, as well as reduce defects and the use of chemicals in the arm coil assembly process of hard disk drive manufacturing in the case study company by applying the Cleaner Technology concepts. The four main sequential steps used in this research were: (1) pre-assessment, (2) assessment, (3) feasibility study, and (4) implementation. In the first step, raw data, such as process flows, raw material usage and defects data were collected. In the second step, the loss during production and causes of loss were analyzed. Opportunities to reduce raw material, chemical and energy wastage could then be recommended. The next step was to evaluate the feasibility and potential benefits of a particular Cleaner Technology opportunity. Finally, in the last step, after a thorough evaluation and implementation of the opportunities to apply clean technology, the results showed that Arm Coil defects could be reduced by improving the production process using the ECRS technique. ECRS stands for Eliminate, Combine, Rearrange and Simplify. This improvement reduced arm coil defect rates from 0.48% to 0.15%, thus saving approximately 139,638 Thai Baht per month. In addition, production stoppage decision made by workers was used to increase employee involvement in defect detection. Allowing workers to participate in such a decision was an effective way to reduce defect rate and could motivate workers to produce a better quality job. This resulted in arm coil defects reducing from 0.41% to 0.025%, with about 74,562 Thai Baht per month saving. Additionally, an increase in the efficiency of electricity consumption occurred, by increasing the speed of the infrared oven conveyor belt, improving average productivity from 533 pieces/hour to 560 pieces/hour, without adversely affecting product costs and quality, thus producing products of up to the value of 206,242 Thai Baht per month. Furthermore, the new 2-layer curing equipment was designed, developed and used. The upper layer of the equipment was used for arm curing and the lower layer was used for arm coil curing. The result of using such equipment led to a saving of approximately 25,502 Thai Baht per month in electricity costs, without affecting product quality. The outcome of these Cleaner Technology applications was that total costs of approximately 239,702 Thai Baht per month were able to be saved. Presented at the International MultiConference of Engineering and Computer Sciencetists 2010, March 17-19, 2010. 440 Application of Fuzzy Analytic Hierarchy Process for Supplier Selection of Automobile and Electronic Industries Pongsak Holimchayachotikul1, Komgrit Leksakul2 and Surakrit Nattaradol2 1 College of Arts, Media and Technology, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Industrial Engineering, Faculty of Engineering , Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT This paper has three main objectives, the first is to know the priority of each industry under the same criteria. Secondly, we have constructed the systematic purchasing evaluation. Lastly, this information can be used for suppliers’ improvement process. Sub-criteria of the paper are Product price, product quality, process technology, process capacity, delivery time, and credit period. After we have collected all essence data, gathered, and evaluated them, the result of this thesis presents a priority of each criterion. In electronic industries, they will focus on product quality, delivery time and product prices, respectively, while automobile industries will focus on product prices, product quality and process capacity respectively. After conducting case studies, the appraises can prove that the theory was absolutely accurate. Thus, this paper has also illustrated the potential of FAHP to solve multi-criteria decision problems. Key words: Fuzzy Analytic Hierarchy Process, Supplier Selection, Automobile and Electronic Industries. Presented at 2010 2nd International Conference on Information and Multimedia Technology (ICIMT 2010). 441 Automatic Detection of Electricity Pylons in Aerial Video Sequences Jittichat Tilawat1, Nipon Theera-Umpon1 and Sansanee Auephanwiriyakul2 1 Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Automatic power transmission line inspection with aerial video surveillance requires that the vehicle-mounted camera can automatically locate electricity pylons. In this paper, a new approach for locating electricity pylons within video sequences is proposed. Straight lines in each video frame are extracted with a two-dimensional separable infinite impulse response (IIR) filter and the Hough transformation. A technique for selection of lines representing the electricity pylons is also proposed. The experimental results from a set of real-world video images are shown in term of the receiver operating characteristic (ROC) curve to verify the algorithm’s detection performance. The ROC curve shows that the proposed algorithm performs very well and can very much help in the automatic electricity pylon detection system. Presented at International Conference on Electronics and Information Engineering 2010, Aug.1-3, 2010. 442 Bus Stop Selection for Bus System of Large IndustrialFactory Raweeroj Jintawiwat Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200 Thailand. Abstract This research is to select bus-stop for bus route system of large scale factory using clustering techniques. This problem's solution is to find optimal number and position of bus-stop. Moreover, another objective is to minimize total distance between residence and bus-stop and number of bus-stop while distance between residence and bus-stop must less than 2 km. In addition, Global positioning system (GPS) is used to indentify factory, bus stops and possengers' residence. The Problem instances were demonstrated by real data, 5232 workers of large scale factory of Thailand. K-Mean, Fuzzy C-Mean, Competitive Learning and Maximin are perform to solve this problem by changing the number of bus-stop into 100-1000 and use center of group of workers to be the bus-stop. The experimental results show that the best solution is K-mean, clustering center initialed by Maximin, with 500 bus-stop. Furthermore, the best solution show total distance is 1,063.65 km/shift and reduce 79 percentages, compare with present solution. Presented at 2010 2nd International Conference on Information and Multimedia Technology (ICIMT 2010). 443 Characterization on Properties of Modification Gelatin Films with Carboxymethylcellulose Fasai Wiwatwongwana1 and Somchai Pattana1,2 1 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Biomedical Engineering Center, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Effects of carboxymethylcellulose (CMC), a derivative of cellulose, blended with biopolymer gelatin films has been studied. The films were fabricated by blending CMC with gelatin solution in various ratio and casted on glass cover slips. Thermal and chemical crosslinking techniques were used to induce conjugation of free amide and carboxyl groups in protein structures of the films. Physical and mechanical properties of different gelatin/CMC films were characterized by Atomic Force Microscope (AFM) which scans on film surfaces and evaluates their elasticity. The physical structures of the films from AFM analysis indicated that increasing of CMC ratio effected in more aggregated of the protein structures of all the films. The analysis mechanical properties demonstrated that increasing of CMC ratio in gelatin/CMC films resulted gradually increasing in modulus of elasticity compared to pure gelatin films. The physical and chemical crosslinking EDC/NHS in 50 mM MES buffer in 40% ethanol improved in mechanical strength of all the gelatin/ CMC films by increasing in modulus of elasticity with an average at 62.71±1.69 kPa and 63.24±0.92 kPa, respectively compared to pure gelatin film. These results suggested that using CMC as an additive and crosslinking techniques including thermal treatment and EDC/NHS as a crosslinking agent strengthened in protein structures which enhanced in mechanical properties of gelatin. The additive of CMC had tendency to display some interesting properties for applying in biomedical applications. Key words: gelatin, carboxymethylcellulose, film, Atomic Force Microscope, Modulus of Elasticity Presented at the First TSME International Conference on Mechanical Engineering, 20-22 October, 2010, Ubon Ratchathani. 444 Design of Signal Control by Using FPGA for Indirect X-Ray Detector Couple CIS Image Sensor Suranan Noimanee Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract This research is the simulation phase for a design of indirect x-ray detector by using contact image sensor (CIS). In this phase present how to create signal controls. First, SP signal MCLK signal will be created for contact image sensor part. In addition, ADCCLK signal, SCLK signal and CDSCLK2 will be generated for analog front-end part. This research was selected Field Programmable Gate Array (FPGA) for generate signal control which created by counter method from oscillator (50MHz). All signal control of this phase will lead to the second phase which should record raw data from contact image sensor and transfer convert raw data by analog front-end. Then displayed all signal in computer. Key word: Indirect X-ray Detector, Signal Control, Contact Image Sensor (CIS), Field Programmable Gate Array (FPGA). Presented at the 3rd Biomedical Engineering International Conference (BMEiCON 2010) 27-28 August, 2010. Kyoto, Japan. 445 Development of Wireless Videonystagmography Recording for Medical Diagnosis Suranan Noimanee Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract We designed and development of IEEE802.15.4 standard wireless videonystagmography recording interface to notebook computer for helping the physician to diagnosis his/her patient, which is based on Electro-oculography transceiver module with very small transmission power. In this paper we use wireless videonystagmography (WVNG) technologies for testing inner ear and central motor functions. WVNG testing is considered the new standard for testing inner ear functions over electro-nystagmography, because WVNG measures the movements of the eyes directly through wireless cameras. WVNG testing is more accurate, more consistent, and more comfortable for the patient. By having the patient more comfortable and relaxed, consistent and accurate test results are more easily achieved. We presented and active eye tracking system that users can use it in unrestricted posture with different angle and distance to notebook computer. It can calculate the camera target position. We can choice any user to track eye gazing. The results provide a useful wireless CCD camera for large motion and long distance usage. Key words: IEEE802.15.4 standard, WVNG (wireless videonystagmography), EOG (Electro-oculography), active eye tracking system, notebook computer, wireless CCD camera. Presented at Biomedical Engineering International Conference, The 3nd BMEiCON 2010 BMEiCON-2010, Kyoto, Japan. August 27-28, 2010. 446 Drying kinetics of Longan Flesh at High Temperature Sarapee Chunthawom, Siva Achariyaviriya, Aree Achariyaviriya and Kodkwan Namsanguan Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The objectives of this research were to study the theoretical drying kinetic equation of longan flesh drying at high temperature and to develop the diffusion models with Armenius-type equation. The longan of E-dor variety (Dimocarpus Longan Lour.) was used in this study. The sample sized of 25-30 mm. in diameter and the initial moisture content of 470-510 %db were tested. The experiments were carried out in a hot air dryer at the drying air temperature in a range of 100-130°C and a constant air velocity of 0.7 m/s. This longan was dried until final moisture content of 18 %db. The effective diffusion coefficient was determined by fitting the experimental data to drying kinetic equation using least square method. It was found that Model 2, which the Arrhenius factor (DO) was a second-degree polynomial function of moisture content, gave the highest value of R2 and the lowest value of MRS. The moisture ratios calculated from each model were compared with experimental result. It could be concluded that the moisture ratios calculated by using Model 2 were accurate throughout the drying process. Presented at the 6th Conference on Energy Network of Thailand, 5-7 May 2010, Srinakharinwirot University, Bangkok. 447 DYNAMIC BEHAVIOR OF ELECTROCNIC CABINET Olaree Nawikkawanit and Chayanon Hansapinyo Department of Civil Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Cabinet for electronic equipment is required to protect internal electrical equipments from damage. Especially in seismic zone, structural safety and serviceability of cabinet during a potential earthquake has to be investigated. This paper presents vibration test for frequency response function (FRF) to determine natural frequencies of the cabinet. Three dimensional finite element analysis was also adopted to study dynamic response of the cabinet. The dynamic characteristics of the cabinet from the two results were then compared with that value recommended in standard of practice in producing the cabinet for seismicity zone. From the vibration test, the natural frequencies of the first three modes are 9.2 Hz, 19.2 Hz, and 23.6 Hz. The finite element analysis shows the similar trend of the natural frequencies of the first three modes which are 10.9 Hz, 23.8 Hz and 32.9 Hz. From the vibration study, it can be said that the studied cabinet can be used in a seismicity area in which the fundamental natural frequency is higher than the required minimum standard frequency of 6 Hz. Key words: Electronic cabinet, Earthquake, Finite element analysis, Mode shape Presented at 3rd International Conference on Geoinformation Technology for Natural Disaster Management & Rehabilitation, Oct. 19-20, 2010. 448 EARTHQUAKE LOSS ESTIMATION FOR CHIANG MAI CITY Chayanon Hansapinyo and Bhuddarak Charatpangoon Department of Civil Engineering, Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Chiang Mai, the second largest city in Thailand, is located about 700 kilometers north of Bangkok. The city has a very strategic position, because its location is at the center of Upper Northern Region, connected to many important cities and countries in Mekong region i.e. Burma, Laos and China. Seismically, although the country has long been realized as low seismicity, the present historical seismicity has proved the city to be classified in moderate risk zone. However, Chiang Mai has never experienced devastating earthquake in at least the past 100 years, resulting in a very low awareness to earthquake risks. Hence, this paper presents a spatial study on seismic performance of buildings in Chiang Mai city to establish earthquake scenario under a magnitude of 6.0 with two assumed epicenters leading to two corresponding seismic scenarios. The results on the building damages show that the most suffered area was occurred in a high dense building stock zone. In the 1st scenario, the damage of the building is about 400,000 m2 in every 1 km2 in or 32% of the entire area. In 2nd scenario, the building’s damage is about 1,000,000 m2 in every 1 km2 or 80% of the study area. As a result, this information is good sources to the government officer to manage and used as a tool to prepare the preparedness plan against this kind of disaster. Key words: Loss estimation, Earthquake, Preparedness plan, Chiang Mai, Building damage Presented at Third International Conference on GIT4NDM 2010. October 19-20, 2010, Chiang Mai, Thailand. 449 Effect of DSM in Cold Storage on Product Properties and Electricity Usage Chutchawan Tantakitti1 and Preecha Sriprapakhan2 1 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Energy Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Frozen packages of shrimp and bean are stored in a cold room where temperature is set at -20°C during on-peak and switched to -40°C during offpeak period to reduce energy demands. In this study, effects on frozen products properties and electricity requirement were investigated. Temperature at various positions in the cold room as well as core and surface temperature of products and electricity requirement are monitored. All measurements have been undertaken and recorded every 6 minutes for at least 9 months. Products are sampled at 0, 1, 3, 6 and 9 month to evaluate physical and chemical properties, and against both control and international food standards. Preliminary results show that significant electrical peak reduction of about 54% can be achieved while frozen products quality remained acceptable, in accordance to international standard. Key words: Frozen shrimp, Bean, DSM, Thermal storage. Presented at 23th International Conference on Efficiency Cost Optimization Simulation and Environmental Impact of Energy System, June 14-17, 2010. 450 Effect of Powder Sized on Heat Transfer Characteristics of Miniature Sintered-Wick Heat Pipe Sophon Sinsang1, Phrut Sakulchangsatjatai1, Pradit Terdtoon1 and Nattapong Sangsirakoup2 1 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Thermal Solution Department, Fujikura Electronics (Thailand) Ltd., Navanakorn Factory 1, 101/2 Navanakorn Industrial Estate, Paholyothin Rd., Klongnung, Klongluang, Patumthani 12120, Thailand. Abstract The objective of this study is to experimentally and numerically detemine the effect of powder sizes on the void fraction, which subsequently affect the heat transfer characteristics, of miniature sintered-wick heat pipe usually applied in the notebook computer. The powder size of the wick is a profound influence on the heat transfer performance, as well as the economic aspect, of the heat pipe. By using computer program to simulate the void fraction of the sintered wick made of two sizes of metal powder, and accordingly the mixture fraction of such metal powder, the optimum throughput heat transfer of the miniature heat pipe can be predicted. The inputs of the simulation program are size specification, the thickness of copper powder and powder fraction. The heat transfer characteristics of the heat pipe will be dtermined by firstly obtaining the void fraction of such sintered wick. Then the heat transfer rate will be calculated from the void fraction. The optimum void fraction can be solved as the point at which the void fraction and heat rate are appropriate on the basis of economic consideration. In order to validate such calculation, the experiments have been conducted with several kinds of metal powders. The wick empolys the copper powder with range of diameter of 150-212 and 250-297 micron respectively by wick molding with sintering process. The miniature heat pipe with outside diameter of 6 mm and the total lengths of 200 mm was used. The wick thickness was fixed of 0.46 mm. Water was used as a working fluid. From the result, the heat transfer performance at normal state of the heat pipe with powder fraction of 1:3 has found to be as hish as 25 W which is quite closed to the predicted one. Key words: heat transfer characteristics, miniature sintered, wick, heat pipe Presented at the 2nd RMUTP International Conference 2010. 451 EFFECTS OF SEISMIC FORCE ON MAE THALOP LUANG DAM Bancha Sinsuksate1, Chayanon Hansapinyo1, Kyung-Ho Park2 and Pennung Warnitchai2 1 Department of Civil Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Engineering and Technology, Asian Institute of Technology, Thailand. ABSTRACT This study is aimed to investigate the effects of earthquake loading on Mae Thalop Luang Dam by using 2D plane strain nonlinear finite element analysis. The finite element modeling adopting gravity turn-on method and the incremental method were implemented for this study. Dynamic analysis under three selected time history records, i.e. long duration, short duration and impulsive, were performed. From the results, the gravity turn-on method gives about 2~3 times higher responses compared to those from the incremental method. For example, under the three earthquakes, the maximum crest settlement indicating water overflow during an earthquake are respectively 11.9, 11.5 and 5.6 cm for the gravity turnon model and 4.5, 2.8, 2.1 cm for the incremental model. Moreover, the long duration earthquake created higher effects compared to other shorter duration earthquakes Key words: Earthdam, Earthquake, Safety, Chiang Mai, Mae Thalop Luang dam Presented at Third International Conference on GIT4NDM 2010. October 19-20, 2010, Chiang Mai, Thailand. 452 Experimental Investigation of Closed Loop Oscillating Heat Pipe as the Condenser for Vapor Compression Refrigeration Pracha Yeunyongkul1, Phrut Sakulchangsatjatai1 and Afshin J. Ghajar2 1 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 School of Mechanical and Aerospace Engineering, Oklahoma State University, Stillwater, Oklahoma, USA, 74078-5016 ABSTRACT The aim of this article is to experimentally investigate the application of a closed loop oscillating heat pipe (CLOHP) as the condenser for a vapor compression refrigeration system. Split type air conditioner for residential use has two major disadvantages. First, it has a large pressure drop in the condenser caused by the flow of refrigerant inside a very small tube which affects compressor power and results in a decrease in the coefficient of performance (COP). Second, a large amount of heat is rejected to the surroundings since the refrigerant has to condense after passing through the condenser. To decrease pressure drop and recover heat rejection from the condensing process, this study considered using CLOHP instead of the conventional condenser in the split type air conditioner. The refrigeration capacity was set at 12,500 Btu/h (3.663 kW) with R22 as the refrigerant. The simulation of CLOHP condenser for the establishment of the optimum size of the vapor compression refrigeration system was performed using the thermoeconomical method. For the optimum system size, it was found that water as the working fluid provided the highest net savings. The optimum size of the system with water as the working fluid consists of a 0.08 meter of evaporator section length, a 0.1 meter of condenser section length, pipe with an inner diameter of 2.03 millimeter, and 250 turns. Therefore, these sizes were selected to construct the CLOHP condenser. The experimental results were obtained and compared with the conventional condenser. It was found that COP of CLOHP condenser with a heat load of 800 W was decreased by about 32.4 but the pressure drop of the CLOHP condenser was lower than that of the conventional condenser by about 91.2... In addition, the energy efficiency rating of the CLOHP condenser was higher than the conventional condenser by about 13.4%. Finally, the outlet temperature of the cooling water which recovers heat from the condenser section of CLOHP, was increased by about 3 ..C. The same trend was also observed for the heat loads of 900 W and 1,000 W. Presented at International Refrigeration and Air Conditioning Conference at Purdue, July 12-15, 2010. 453 Impact of Work-Rest Period on Mental Fatigue in Inspection Task with Microscope: Case Study of Hard Disk Drive Component Manufacturing Company Rungchat Chompu-inwai and Kanitha Yajom Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract In recent years, inspection has declined in importance as statistical process control has assumed a more prominent role in the quality function. However, product quality inspection is still an important task in the production of hard disk drive component manufacturing. In the case study company, which produces hard disk drive component, inspection occurs at many points in a process. Inspectors perform 100% inspection using microscope for many hours without adequate break. After continually monitoring the performance of inspectors, it was found that certain nonconformities were more difficult to find, the number of nonconformities reported was unusual, and inspectors varied in their abilities. Turnover rate of the quality inspection division in this company is high. Therefore, this research aims at determining the impact of working duration and resting time on reducing the mental fatigue in inspection task with microscope in the hard disk drive component manufacturing. Five types of working duration and resting time were studied and compared using Randomized Complete Block Design of Experiment with two replicates. A variety of data collection tools were used to assist in the data collection. The data collection tools used included bipolar questionnaire, reaction time, and critical flicker frequency (CFF). The analysis of variance (ANOVA) was conducted. The result showed that, the work-rest period type significantly affects the mental fatigue reduction as measured by questionnaire scores and reaction time. However, the work-rest period type significantly does not affect the mental fatigue reduction as measured by CFF. Presented at The International MultiConference of Engineering and Computer Sciencetists 2010, March 17-19, 2010. 454 Modeling and Validation of a Single-Cell PEMFC Chaiwat Pumchat1, Taworn Benjanarasuth1, Nirut Naksuk2, Waravut Printrakoon2, and Kasemsak Uthaichana3 1 Faculty of Engineering, King Mongkut’s Institute of Technology, Ladkrabang, Bangkok 10250, Thailand. 2 National Metal and Materials Technology Development Agency, Pathumthani 12120, Thailand. 3 Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT In this paper, the steady-state mathematical model of a single-cell Proton Exchange Membrane Fuel Cell (PEMFC) is derived from the electrochemical reactions. The governing equations are combined to form the overall single-cell PEMEC model using Matlab/Simulink. The experiments are conducted and the cell voltage, power, efficiency and humidity at various operating current levels are investigated and compared to the simulation results. The model has been validated against the obtained experimental results. It was found that the simulations results based on the presented model are consistent with the experimental results with minimal error. Presented at 4th Asia International Symposium on Mechatronics, Singapore, December. This work is supported in part by Engineering Research Fund, Sensor Cluster, NECTEC, Thailand. 455 Noise covariance estimation using dual estimation for disturbance storm time index application Boonsri Kaewkham-ai and Kasemsak Uthaichana Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The disturbance storm time (Dst) index is used for predicting the geomagnetic storm that can affect many systems on earth. The application of the dual unscented Kalman filter (DUKF) to improve the quality of the D st index prediction by simultaneously estimating the process noise covariance is set forth in this paper. In DUKF, two unscented Kalman filters (UKFs) are run in parallel. The UKF applied to a model-based Dst index prediction is so called a state estimator; while the other, a parameter estimator, is for identifying and recursively updating the process noise covariance. The performance comparison between the traditional UKF with fixed constant values of the process noise covariance, and the DUKF are examined. The actual all Dst and the Dst data during the storm (below -80 nT) are used to assess the quality of the predictions. It is found that root mean square error (RMSE) of Dst index prediction using the DUKF is lower than that of the UKF with fixed constant process noise covariances. Specifically, RMSEs of the DUKF are 6.5816 for all Dst and 18.0615 for Dst below -80 nT, whereas, the prediction using a fixed constant process noise covariance yield RMSEs of at least 6.6678 and 19.3954 for all Dst and Dst below -80 nT, respectively. Hence, the DUKF outperforms the traditional UKF with fixed constant process noise covariances in this study. Presented at 11th International Conference on Control, Automation, Robotics and Vision, Singapore, December. This work is funded by Faculty of Engineering, Chiang Mai University. 456 PERFORMANCE COMPARISON OF HEAT PUMP DRYER FOR SMALL INDUSTRY BY DIFFERENT CONTROL METHODS OF HOT AIR TEMPERATURE Eakrit Kaewjaren and Siva Achariyaviriya Department of Mechanical Engineering, Faculty of Engineering, Chaing Mai University, Chiang Mai 50200, Thailand. Abstract The objective of this research is performance comparison of heat pump dryer with two control methods of hot air temperature. The first method is the bypass working fluid heat pump dryer (BWF-HPD) which used for reject the extent heat to external condenser. The second method is the variable speed drive heat pump dryer (VSD-HPD) which used for control the speed of compressor by inverter for adjust a suitable flow rate of refrigerant mass for heat load inside the dry chamber. The material in this research was the 3 mm thickness slide bananas which had 33.6 kg initial weight and the compress cloth fiber sheet which had 31.07 kg initial weight. The working system of heat pump dryer was the closed loop system at fraction of evaporator was 70 % and air velocity was 1.252 m/s. The experiment at 60°C shown that the specific moisture extraction rate (SMER) and coefficient of performance (COP) of VSD-HPD method higher than BWF-HPD method. Presented at The 9th Conference on Energy, Heat and Mass Transfer in Thermal Equipments and Process. Prachuabkirikhan, 2010. 457 Power Transfer Capability Enhancement with Multitype FACTS Controllers Using Particle Swarm Optimization Suppakarn Chansareewittaya and Peerapol Jirapong Department of Electrical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT In this paper, particle swarm optimization (PSO) is proposed to determine the optimal allocation of multitype FACTS controllers to enhance power transfer capability of power transactions between source and sink areas in power systems. The particularly optimal allocation includes optimal types, locations, and parameter settings. Three types of FACTS controllers including unified power flow controller (UPFC), thyristor-controlled series capacitor (TCSC), and static var compensator (SVC) are used in this study. The objective function is formulated as maximizing the benefit to cost ratio. The benefits mean increasing total transfer capability (TTC) with deducting system losses while the cost are the investment of FACTS controllers installation. Power transfer capability determinations are calculated based on optimal power flow (OPF) technique. Test results on the modified IEEE 30-bus system from PSO are compared with those from evolutionary programming (EP). The results show that PSO can be used to determine the optimal allocation of multitype FACTS controllers. The optimally placed OPF with FACTS controllers using PSO enhance higher power transfer capability than those from EP. Moreover, PSO gives higher benefit to cost ratio and faster convergence than EP for all transfer areas. Therefore, this installation is worthwhile and beneficial for the decision making of investment costs and further expansion plans. Key words: particle swarm optimization; FACTS controller; optimal allocation; power transfer capability Presented at TENCON2010-2010 IEEE Region 10 Conference, Fukuoka, Japan. November 21-24, 2010. 458 PREDICTION OF INITIAL NATURAL DETERIORATION OF HISTORICAL BRICK MASONRY STRUCTURES BY ACCELERATION TEST USING SULFURIC ACID Pitiwat Wattanachai and Bancha Jiwdet Department of Civil Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This research is aimed to study deterioration and to formulate the equation for prediction of deterioration of bricksand lime mortar, and to study a relationship between compressive strength and deterioration depth from exposure surface and time.The specimens were closed 5 surfaces with epoxy for one side exposing. Then, they were tested for compressive strength of brick having size of 5×5×5 mm3 at six layer depths and mortar having size of 10×10×10 mm3 at four layer depths for a period of 24 weeks. There were three cases of testing program; which were performed (1) natural deterioration process, (2) accelerated deterioration process and (3) both natural and accelerated processes. After the experiment, the results of chemical and physical properties showed that the sulfur compounds and sulfate salts crystal inside specimens. For the natural deterioration process, it was the weight and compressive strength of brick and lime mortar gradually increased; and for the accelerated deterioration process, the first 12 weeks, it can be indicated that weight and compressive strength increased but the both value decreased after 12 weeks. Additionally, the most-deteriorated layer depth was exposured surface. Thus, deterioration of bricks and lime mortar depend on layer depth and exposured time. Presented at The 6th Asian Concrete Federation International Conference, Oct. 20-20, 2010. 459 PRORISK: A Project Oriented Risk Management Model Boriwat Suebkuna and Sakgasit Ramingwong Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50202, Thailand. ABSTRACT There have been continuous attempts to develop a perfect risk model for software project management. Yet, most of these attempts fail in addressing critical aspects such as lack of practical procedure, incomplete focus on risk facets, or missing links between software project life cycle and risk management. This paper proposes a model and framework to solve this problem by integrating various risk management models on standard project management life cycle. This leads to a practical approach which can be easily understood and efficiently applied in any software projects. Presented at The 2010 International Computer Science and Engineering Conference, Nov. 17-19, 2010. 460 SEISMIC EVALUATION OF SCHOOL BUILDINGS IN CHIANG MAI Parinya Khamwongpin1, Chayanon Hansapinyo1,2 and Bhuddarak Charatpangoon1,2 1 Department of Civil Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Due to the low self-defensive of school children, the seismic safety and collapse mechanism of school building have been wildly raised including those in Chiang Mai, the second largest city in Thailand. It is due to the fact that the city contains a number of schools and the area is prone to some moderate seismicity. In this paper, the seismic capacity evaluation of the existing school buildings is presented. The study first conducts the Rapid Visual Screening (RVS) of existing school building inventory in Chiang Mai to identify and rank buildings that are potentially seismically hazardous. Then, more detailed analysis by using nonlinear pushover analyses under ATC-40 and FEMA-273/274 of a representative building is performed. The RVS results show that most school buildings are concrete frame with unreinforced masonry (C3). The RVS final scores indicate a greater risk with the negative factors i.e. pre-code design, soil condition and irregularity. For the pushover analysis, typical school building with three story high and one-bay lateral frame is selected for the investigation. It can be seen that the failure of school building is initially occurred at 2nd story columns with the occurrence of minor cracking and then propagate to flexural yielding. Finally, the 1st story columns are failed by flexural bending and the capacity curve shows suddenly decrease. The ADRS represents the performance of the typical school building is ranged from Life Safety to Collapse Prevention. This refers to the underperformance of this typical school building. Key words: School buildings, pushover analysis, seismic evaluation, Chiang Mai, Rapid Visual Screening Presented at Third International Conference on GIT4NDM 2010. October 19-20, 2010, Chiang Mai, Thailand. 461 Space Vector Pulse Width Modulation (SVPWM) of Two-Level Inverter Using TMS320F2812 Digital Signal Processor (DSP) Yuttana Kumsuwan Department of Electrical Engineering, Faulty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract This paper describes an investigation of space-vector pulse width modulation (SVPWM) for two-level inverter. The technique is developed by considering both continuous and discontinuous pulse width modulation techniques. The implementation of SVPWM in the V/f control of the induction motor is discussed, and it is found that for SVPWM using currently available TMS320F2812 digital signal processor (DSP), the SVPWM algorithms were directly programmed in MATLAB/ Simulink software package. Smooth operations during transition from continuous mode to discontinuous mode are demonstrated through simulation and experimental results. Presented at The 33rd Electrical Engineering Conference (EECON-33), Chiang Mai, Dec. 1-3, 2010. 462 The Assessment of Carbon Dioxide Elasticity by Using the Input-output Method: The case of Thailand Krissana Romchaiyaphruk, Wongkot Wongsapai and Itthichai Preechawuttipong Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The primary energy supply in Thailand has been mainly depended on imported fossil fuels. In 2005 commercial energy including coal, oil and natural gas shared about 83.5% in the total primary energy supply of the country. To see that current energy production and use patterns rely heavily on the burning of fossil fuels. This means that the Thailand is using mainly energy sources which inevitably produce CO2 emissions, a key factor in the greenhouse effect and of the resulting climate change problem. Thus, the current paper details a proposal to study the assessment of CO2 elasticity for the Thailand economy by using the input-output (IO) analysis. Moreover, this study also analyzes the impacts of the decreasing in final energy demand and changes of energy saving policy (Oil and Coal) in Thailand. For the analysis, all 180 sectors from the Thailand input-output table since 2005 will be carefully rearranged and reduced to about 20 sectors. It is the goal to use the developed model to assess the impacts of the policy on the economy; for instance, the impact on the final energy demand, the impact on the manufacturing and economic sectors. Key words: Input-output analysis, CO2 emissions, CO2 Elasticity, Thailand Presented at The 3rd Technology and Innovation for Sustainable Development International Conference (TISD2010), Faculty of Engineering, Khon Kaen University, Thailand. Mar 4-6, 2010. 463 The Furniture Design Research by Creating with contemporary Cultural Value for Value Added and Market Differentiated Pitsamai Arwakulpanich Thai Art Department, Faculty of Fine Arts, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Phrae Province is very famous in teakwood furniture for a long time. But recently, furniture products face a problem in copying each other because the community can not bring their identity through their furniture products. Causing the problem that furniture products exceed the requirement in the market. This research has the objective for seeking design trend to build the value on their furniture products by using Lan Na wisdom. Enhance the prominent point and value added of furniture products in the sense of designing Lan Na style. The result of the study can summarize the way of designing furniture products in the contemporary way by using the applied geometric analyzing from Lan Na form, which is short, the fort and obtuse go out the top as V-shaped such as Ruen Kalae which is unique to the North. For example, using the concept of living area in Ruen Kalae like toen and porch, designing furniture that use in the modern living room.? Presented at CMU Art Center, Chiang Mai University from 3th-30th October 2011. Honorable mention from Lanna Creative Economy Award under the Creative Economy Project by the group of North province 1 on Friday 5th August 2011. 464 Biomethane: An Alternative Green Fuel to CNG Koonaphapdeelert Sirichai1, Kantha Uthen2 and Aggarangsi Pruk3 1 Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Energy Research and Development Institute-Nakornping, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT A pilot-scale biogas upgrading system based on pressurized water scrubbing technology was installed and tested in a pig farm in Chiang Mai, Thailand, to convert ordinary biogas from pig manure to biomethane at the production capacity of 20 1Nm3raw biogas per hour. The upgrading system was continuously run for six calendar weeks with good stability and was able to produce biomethane containing approximately 85% by vol. of methane with Wobbe Index of 40.35 MJ/ m3. The biomethane properties were tested using ASTM methods and found to meet the regulatory CNG standard adopted by Thailand Ministry of Energy. The capital cost of the biomethane production system was estimated to be 27.7 million Baht for a capacity of 333 Nm3raw biogas/hr producing 186.5 kg of biomethane/ hr at a unit cost of compressed biomethane gas of approximately 12.28 Baht/kg. 1 Nm3 refers to Normal-cubic meter at 0°C 1, atm. Presented at “The 7th International Conference on Automotive Engineering (ICAE7 March 28-April 1, 2011”, Challenger, Impact, Muang Thong Thani, Bangkok, Thailand. Supported by Four-T Farm, Chiang Mai, Thailand. 465 Participation of the Communities to Process Food in GHP / GMP Guidlines : A Case Study of Red Chili Paste and Tua Noa Products Community Enterprise Narong Sikhiram Home & Communities Division, Faculty of Humanities, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The study was conducted at 111 Moo 2, Ban-Mae, San Pa Tong District, Chiang Mai, Thailand. This research aimed to develop a hygienic process of making Nam-Prig-Ta-Dang (red chili paste) and Tua Noa (as Natto) Products. Another aim was to establish a training center. The study was carried out as a participatory study, which included seminars and workshops on hygienic conditions for food production for the participants. The trainings also covered other food production related topics; including, food production and development, food packaging selection, shelf-life, food preservations, and personal hygiene. The participants in the study participated in a focus group in which they collaborated in creating quality manuals. They also took part in analyzing the problems and the possible solutions as well as a SWOT analysis of their community enterprise. In addition, they collaborated in designing a factory building. In the collaborative learning process, they shared and learned from one another, generated new ideas in which they gained confidence in solving problems by process, in the planning and acting processes, as well as sharing the benefits. Furthermore, this learning process has empowered the participants in solving problems by building multilateral networks. The participants also became bonded in the process. In addition, new knowledge was created. Therefore, it could be concluded that this participatory method (i.e. this research study) has empowered and caused sustainability to this Community. Key words: Participation, Supporting, Processed Food, Community Enterprise, GHP, Red Chili Paste Presented at Oral Presentation in 16th ARAHE International Biennial Congress THEME: "The Home Economist: Towards a More Sustainable, Commitment-Based, Family-Focused, Perspective in Addressing Global Issues" on July 25-29, 2011 at the Asian Institute of Management Conference Centerence Center Manila (ACCM) in Makati City, Philippines. 466 The Abstracts unpublished DETERMINATION OF ORGANOCHLORINE PESTICIDES LEVEL IN FARMER SERA FROM CHIANG MAI PROVINCE Phraepakaporn Kunnaja, Khanittha Taneyhill and Warunee Kumsaiyai Department of Clinical Chemistry, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Organochlorine pesticides are a large class chlorinated hydrocarbon chemicals. Organochlorine pesticides break down slowly in the environment and always accumulate in the human body. The objectives of this study were to investigate the normal ranges of organopchlorine pesticides contamination and the differences between the occupational exposed and occupational non-exposed to pesticide. To estimate levels of organochlorine residuals, we determined serum levels of alphabenzenehexachloride (alpha-BHC), gamma-benzenehexachloride (gamma-BHC), beta-benzenehexachloride (beta-BHC), delta-benzenehexachloride (delta-BHC), heptachlor, aldrin, endosulfan II, 4,4’-dichlorodiphenyltrichloroethane (4,4’-DDT), endrin aldehyde, methoxychlor, endosulfan sulfate and endrin ketone in 53 volunteers from Chiang Mai and Lumphun provinces, Thailand. The volunteers were divided into two groups. First group was occupational non-exposed to organochlorine pesticide (4 males, 22 females, 27-69 years old) and was defined as a normal group. The second was investigated in a population of agricultural workers chronically exposed to organochlorine pesticides and was defined as a risk group (8 males, 19 females, 39-64 years old). Serum levels of organochlorine pesticides were measured using gas-chromatography with electron capture detector (GC-ECD). The results showed that organochlorine pesticides levels in serum of the risk group tended to be higher than those of normal group. However, these were not statistically different, excepted for alpha-BHC, methoxychlor and endrin aldehyde. The level of alpha-BHC (22.5±2.1 ng/ml) and methoxychlor (30.1±8.3 ng/ml) in the risk group were significantly increased (p value <0.05) when compared with normal group (21.3±2.2 ng/ml and 26.3±1.5 ng/ml, respectively) and the level of endrin aldehyde in the risk group (10.9±7.9 ng/ml) significantly increased (p value <0.001.) when compared with normal group (4.3±1.1 ng/ml). Serum organochlorine pesticides levels in both two groups were not associated with the frequency of vegetable and fruit consumption. Research supported by Faculty of Associated Medical Sciences. Chiang Mai University. 469 DEVELOPMENT OF THE OCCUPATIONAL PERFORMANCE EVALUATION FOR DEMENTED ELDERLY Supawadee Putthinoi and Wannipa Bunrayong Department of Occupational Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this research was to develop the Occupational Performance Evaluation for demented elderly according to the cognitive disability frame of reference of Claudia kay Allen for using in occupational therapy service in these patients. The processes of this research were divided in three steps. First step was to construct the assessment. Second step was finding the content validity by considering form five experts in occupational therapy area. After that the assessment was improved according their comments. The last step was try out that two occupational therapist used this assessment in thirty older people with dementia at Chiang Mai Neurological Hospital. Finally, researchers and occupational therapist discussed and improved the content of assessment for using in occupational therapy clinic. The Occupational performance evaluation for demented elderly was developed for occupational therapist to indicate problems based on occupational therapy perspectives. A successful, complete development assessment should come to be the first step in forming an intervention program. 2010 (unpublished). 470 PREVALENCE OF OBESITY AND COMPARISON OF THE CARDIOVASCULAR FITNESS IN FEMALE UNDERGRADUATE STUDENTS, FACULTY OF ASSOCIATED MEDICAL SCIENCES, CHIANG MAI UNIVERSITY Nuanlaor Thawinchai1, Ajchamon Tummachai2 and Pavena Intaraksa3 1 Department of Physical Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. 2 Program in Forensic Science, Graduate Student School, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Bumrungrad International Hospital, 33 Sukhumvit 3 (Soi Nana Nua), Wattana, Bangkok 10110, Thailand. ABSTRACT The purposes of this research project were to study prevalence of obesity and to compare the cardiovascular fitness in female undergraduate students, Faculty of Associated Medical Sciences, Chiang Mai University during academic year of 2008. The students were 20-29 years of age. The study was divided in 2 phases: phase I was the prevalence study of obesity and phase II was the comparison study of the cardiovascular fitness using maximal oxygen consumption (VO2max) between the normal body weight group and overweight and obesity group. The results revealed that there were 39.3% normal body weight, 31.4% overweight and obesity and 29.2% underweight. Fifty-eight and forty-two percent had taken low and high calorie snack respectively, Thirty-one percent sometimes exercised and sixty-nine percent did not exercise. When compared the cardiovascular fitness between the normal body weight group and overweight and obesity group, it was found that the overweight and obesity group was statistical significant lower in VO2max than the normal body weight group (p=0.001). However, both groups had a very low cardiovascular fitness level. Therefore, female undergraduate students, Faculty of Associated Medical Sciences, Chiang Mai University should be encouraged to realize the necessity of the exercise in order to prevent the risks of the cardiovascular diseases in the future Key words: Obesity, cardiovascular fitness, female undergraduate students 2010 (Unpublished). 471 QUALITY OF LIFE AND PERCEIVED HEALTH STATUS AMONG PEOPLE WITH SPINAL CORD INJURY Piya Trevittaya and Porntip Wattanavittawat Department of Occupational Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The study of quality of life and perceived health status among thirty people with spinal cord injury, both genders, who live in their communities was conducted by two questionnaires: SF-36 Thai version and WHOQOL-BREF Thai version. The analysis of Cronbach’s alpha coefficient revealed an acceptable internal consistency of SF-36 Thai version and WHOQOL-BREF Thai version questionnaires in all areas (α = .88 and α = .72 respectively). The results of the study showed the score from SF-36 was lower than Thais’ norm in physical component summary score (PCS) (54.92±14.81 and 63.63 respectively) while as the score of mental component summary score (MCS) was nearly the same level when compare with Thais’ norm (67.73±17.07 and 67.64 respectively). The quality of life among this group of people was classified in the average level (85.03±9.86) when compare to Thais’ norm. The result showed no correlation between general demographic information and the scores from both two questionnaires. However, the correlation between SF-36 and WHOQOL-BREF was found significantly (p≤0.05). These findings were discussed in term of relationship of perceived heath status and quality of life. The hierarchy of components of quality of life among this group of people was also discussed. The suggestion of how to apply the finding in services of occupational therapy was recommend in this study. 2010 (unpublished). 472 THE RESULTS OF THAI ELEPHANT-ASSISTED THERAPY PROGRAM ON BASIC SENSORY-MOTOR PERFORMANCE AND ADAPTIVE BEHAVIOR IN INDIVIDUALS WITH AUTISM: A PILOT STUDY Satiansukpong Nantanee, Vittayakorn Soisuda, Pongsaksri Maethisa, Sung-U Sasitorn, Pedugsorn Mayuree, Phiraban Chaithaya and Sasat Daranee Department of Occupational Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Objectives: The objectives of this pilot study were to examine the result of the Thai Elephant -assisted Therapy Program on (1) basic sensory-motor performance and (2) adaptive behavior in individuals with Autism. Study design: A before and after design. Setting: Thai Elephant Conservation Center, Lampang and Occupational Therapy Clinic, Chiang Mai University. Subjects: Four autistic individuals aged between 11-18 years whose parents gave informed consent. Methods: Sensory-motor performance, including sensory processing, balance and postural control as well as the adaptive behavior before and after providing the treatment program was evaluated. The treatment program was provided under supervision of occupational therapists four days a week continuously for three weeks. Data were analyzed by using descriptive statistics. Results: The basic sensory-motor performance was improved. The mean sensory processing was increased 11.45 percents. The total balance scores of participants were increased 69.16 percents, while the means of postural control in supine flexion and prone extension were increased 6.85 and 12.09 percents, respectively. All four had improvement in adaptive behavior. Conclusion: Under supervision of occupational therapists, the Thai Elephant-assisted therapy program was able to improve the basic sensory-motor performance and adaptive behavior of the four autistic individuals. Key words: Autistic, animal-assisted therapy, occupational therapy, adaptive behavior 2010 (unpublished). 473 USE OF INTERNET FOR EDUCATION OF OCCUPATIONAL THERAPY STUDENTS OF THE FACULTY OF ASSOCIATED MEDICAL SCIENCES, CHIANG MAI UNIVERSITY Supawadee Putthinoi and Jiranan Griffiths Department of Occupational Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this research was to study Internet usage for education of occupational therapy students of the Faculty of Associate Medical Sciences, Chiang Mai University. Data and information were collected from questionnaire of 214 students. The findings demonstrate that the most students had experience in using internet more than 3 years and learning how to use by themselves. Most of the students had own computers with an access to the network at home or residence. The purpose of using the internet was for searching information. The most frequent period of time of the most students for using internet was in the night time and spent time 1-2 hours/each. The popular website is www.google. com. All of student accessed website www.ams.cmu.ac.th with the purpose to use teaching evaluation service and web board service, respectively. About using the computer room service of Associated Medical Sciences, most the students from second year student to fourth year student used it whereas most the students from first year did not use it. The majority of the students were high satisfy in item lighting in computer room, moderately satisfy in items readiness and suitability of computer to service, adequacy of computer to service, the efficiency of the network system, knowledge and efficiency of computer instructor and advice about internet using and computer problem solving from computer instructor. All of student accessed website www.reg.cmu.ac.th with the purpose to use registration service and student grade acknowledgement, respectively. Agreement about accessing this website, most of the students found very easy to access and comfortable, 474 moreover most of them recognized this website were very benefit for education in high and highest level. Most of students accessed website KC (Knowledge Creator), but amount of students accessed less than website www.ams.cmu.ac.th and www.reg.cmu.ac.th. Moreover most of students purposed to use this website for education and had agreement this website very useful for education in moderate and high level. Most of students accessed website http://library.cmu.ac.th. Most of first to third year students used item new book whereas fourth year students used item e-Journal. There existed usefulness of this website for education in high level. However, student found some problems during using for example a slow processing and accessing of internet system, a lack of searching skill and using very long time to download data. All of students accessed general websites which were not belong to Chiang Mai University and government to find information for education. Agreement of most of the students about reliability of information from these websites was in moderate level. The suggestions of the internet usage for education were as follows: Associated Medical Sciences and Chiang Mai University should improve homepage and online classroom according to demand and suggestion form students; and lecturers should recognize to develop online resources for benefits of the internet for education. 2010 (unpublished). 475 ALTERED EXPRESSION OF P-AKT, AKT (3 ISOFORMS), MMP, VIMENTIN AND E-CADHERIN IS ASSOCIATED WITH EPITHELIALMESENCHYMAL TRANSITION IN ORAL SQUAMOUS CELL CARCINOMA Anak Iamaroon and Suttichai Krisanaprakornkit Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Aberration of signal transducers in PI3K/Akt pathway has been found in many human cancers including oral cancer and may play a critical role in carcinogenesis of those cancers. Advanced research on the treatments of oral cancer using novel agents targeting on PI3K/Akt signaling pathway are now investigated in many laboratories with promising results. Objectives: The objectives of the present study were (1) to investigate alterations of the protein expression of pan Akt and its phosphorylated form, p-Akt, in oral squamous cell carcinoma (OSCC) tissues of 20 Thai patients, (2) to analyze alterations of the mRNA expression of three isoforms of Akt; Akt1, -2, and -3 and protein expression of pan Akt, Akt1, and Akt2 in OSCC cell lines and human oral keratinocytes (HOK), (3) to analyze alterations of the protein expression of vimentin, E-cadherin, MMP-2 and MMP-9 in OSCC and HOK cell lines. Methods: The expression of pan Akt and p-Akt in OSCC tissues was studied by immunohistochemistry. The mRNA expression of Akt1, -2, and -3 in OSCC cell lines and HOK was analyzed by RT-PCR and the protein expression of pan Akt, Akt1, Akt2, vimentin, and E-cadherin was studied by Western blot assay. The expression of MMP-2 and MMP-9 was analyzed by zymography. Results: The results showed that pan Akt and p-Akt were overexpressed in 95% and 100% of OSCC cases, respectively. We observed more intense expression of pan Akt and p-Akt at the invasive fronts of some OSCC tissues. Pan Akt protein was also overexpressed in all OSCC cell lines in comparison with HOK. Interestingly, Akt1 and -2 mRNA of OSCC cell lines were only constitutively expressed in comparison with HOK. Akt3 mRNA appeared to be minimally expressed in OSCC cell lines and HOK. The Western blot analysis revealed that Akt2 but not Akt1 was overexpressed. Additionally, vimentin and MMP-2 were up-regulated while E-cadherin and MMP-9 were down-regulated. Conclusions: These findings suggested that overexpression of pan Akt particularly Akt2 and p-Akt may be involved with OSCC carcinogenesis and post-transcriptional modification of the expression of Akt isoforms in OSCC may occur. In addition, OSCC cells may undergo epithelial-mesenchymal transition (EMT) since their epithelial makers/phenotypes (E-cadherin, MMP-9) was reduced while their mesenchymal markers/phenotypes (vimentin, MMP-2) were increased. 2010 (unpublished). 476 ANTI-INFLAMMATORY EFFECT OF THE PURIFIED ACTIVE CHEMICALS FROM BOESENBERGIA PANDURATA ROXB. (THAI GINGER, FINGERROOT, OR GKRA-CHAI) IN CULTURED HUMAN ORAL CELLS Suttichai Krisanaprakornkit1, Siriwan Ong-chai2 and Wilart Pompimon3 1 Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand. Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Faculty of Science, Lampang Rajabhat University, Lampang, Thailand. 2 ABSTRACT The objectives of this in vitro study were to study the anti-inflammatory activity of purified active chemicals, isolated from Boesenbergia pandurata Roxb., including pinocembrin and pinostrobin, by testing on mRNA expression for all three isoforms of hyaluronan synthase (HAS), for matrix metalloproteinase-2 (MMP-2), and for matrix metalloproteinase-9 (MMP-9) in primary cultured oral cells. Moreover, we tested the anti-inflammatory activity of pinocembrin and pinostrobin on hyaluronan production and MMP-2 and MMP-9 activities in cell-free conditioned media as well as their cytotoxicity to oral cells. Methods: Pinocembrin and pinostrobin were isolated from Boesenbergia pandurata Roxb. by different solvents according to a standard protocol, and their molecular structures and purity were confirmed. Two types of oral cells, including oral epithelial cells and oral fibroblasts, were cultured following a standard protocol to expand their cell number until they reached 80% cell confluence prior to experimentation. The cytotoxicity of pinocembrin and pinostrobin at different concentrations were determined by an MTT assay, and the non-toxic doses of pinocembrin and pinostrobin were then tested for their anti-inflammatory activity. Oral cells were stimulated with various doses of PMA, a well-known activator, or interleukin-1β (IL-1 β), a pro-inflammatory cytokine, in the presence or absence of 0-30 µg/ml of either pinocembrin or pinostrobin. Subsequently, total RNA was extracted and mRNA expression was analyzed by reverse transcriptase polymerase chain reaction. Furthermore, conditioned media were collected for analysis of hyarulonan levels by ELISA and for analysis of MMP activity by gelatin zymography. Results: It was demonstrated that the percentage of cell survival after being incubated with either pinocembrin or pinostrobin at 0 to 30 µg/ml was higher than 80 and did not significantly differ from untreated control oral cells (P>0.05), indicating that both chemicals were not toxic to both types of oral cells. Both pinocembrin and pinostrobin could inhibit up-regulation of HAS1, 2, and 3 by PMA and IL-1 β in a dose-dependent manner in oral fibroblasts. In addition, both chemicals blocked 477 induction of MMP-9 mRNA by PMA in oral epithelial cells, whereas expression of MMP-2 mRNA in oral fibroblasts was constitutive although cells were activated with PMA or IL-1 β. Consistent with inhibition of gene expression for HAS and MMP-9, both pinocembrin and pinostrobin inhibited an increase in the hyaluronan levels by PMA and IL-1 β in cell-free conditioned media, collected from oral fibroblasts, and an induction of MMP-9 activity by PMA in cell-free conditioned media, collected from oral epithelial cells, in a dose-dependent fashion. However, either of these chemicals did not affect MMP-2 activity at all. It is interesting to note that pinocembrin exerted its inhibitory effects on gene expression, elevated hyaluronan levels, and induced MMP-9 activity greater than did pinostrobin when the same dose was compared. Summary: Purified chemicals from Boesenbergia pandurata Roxb., including pinocembrin and pinostrobin, possess the anti-inflammatory activity, despite their low concentrations in the µg/ml levels, by diminishing gene expression for hyaluronan and MMP-9 as well as blocking raised hyaluronan levels and induced MMP-9 activity, resulting from an inflammatory process in oral cells. This indicates potential applications for these two chemicals in Dentistry. Suggestions for future studies: It is interesting to study the anti-inflammatory effects of Boesenbergia pandurata Roxb. on other biomolecules involved in inflammation. It is also interesting to study other biological activities of Boesenbergia pandurata Roxb., as well as intracellular signaling pathways and receptors on cell membrane that mediate the inhibitory effects of pinocembrin and pinostrobin. 2010 (unpublished). 478 DEVELOPMENT OF NEWLY REGENERATE DENTOALVEOLAR BONE WITH TWIN-TRACK DISTRACTION COMBINED WITH MINISCREW IMPLANTS Eduardo Yugo Suzuki and Boonsiva Suzuki Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Introduction: The purpose of this study was to analyze the maximum insertion (MIT) and removal (MRT) torque values of orthodontic miniscrews inserted in human subjects. Methods: 280 titanium miniscrews were inserted in several sites in the maxillae and mandibles of orthodontic patients to provide skeletal anchorage. MIT and MRT values were assessed with a digital torque gauge. Miniscrews were divided into two groups; pre-drilling (n = 120) and self-drilling (n = 160), based on the placement procedure. A force of 50 g was applied with NiTi coil springs 1 week after placement. Torque values were analyzed by one-way ANOVA. RESULTS: MIT values were significantly higher for the self-drilling miniscrews than for the pre-drilling miniscrews at the midpalatal suture (21.1±2.2 [mean±SD]; 14.5±1.6 N cm) and at the dentoalveolar bone of the maxilla (12.1±3.1; 7.2±1.4 N cm) and mandible (15.7±2.3; 12.4±1.2 N cm). In contrast, MRT values were significantly lower for the self-drilling miniscrews than for the pre-drilling miniscrews at the midpalatal suture (26.9±2.0; 23.2±2.1 N cm) and at the dentoalveolar bone of the maxilla (21.1±2.5; 15.8±3.6 N cm) and mandible (24.5±2.0; 17.9±2.3 N cm). Accordingly, the torque ratio of pre-drilling miniscrews was 91.2% larger than that of the self-drilling miniscrews. Conclusions: These results indicate that a varied amount of osseointegration occurs between miniscrews and the surrounding bone. Relatively lower MIT values were more favorable to the occurrence of osseointegration than were high values. Excessive removal torque increases the risks of miniscrew fracture during removal procedures. 2010 (unpublished). 479 EFFECTS OF DIFFERENT PRIMERS AND ADHESIVE RESINS ON SHEAR BOND STRENGTH OF ORTHODONTIC BRACKET Supassara Sirabanchongkran and Siriwat Wattanapanich Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this study was to evaluate the mean shear bond strength of metal orthodontic brackets bonded to enamel surfaces using two commonly used light-activated adhesive resins and alternation between primers and adhesive resins. The first brand was Transbond™ XT bonding system, and the second one was Enlight® bonding system. Thermocycling and non-thermocycling processes were included in this study. 120 extracted premolar teeth were randomly divided into four groups of 30. The teeth were pumiced, rinsed, and air dried. There were four bonding approaches, and each group was divided into 15 for thermocycling and non-thermocycling process. The first and the forth bonding appraches used the primers and the adhesives resins from each brand. The second methods used the first primer with the second adhesive resin, while the third method used the second primer and the first adhesive resin. After bonding process, all specimens were stored in distilled water for 24 hours at 37°C. Shear bond strength was performed using an Instron® testing machine in group I, III, V, VII. Meanwhile, thermocycling process with 1,000 cycles between 5±2°C and 55±2°C was operated in group II, IV, VI, VIII, and the shear bond strength was also tested. The cross head speed was 0.5 mm/min. ARI scores were recorded under 3X magnification. The mean shear/peel bond strength in the first method was significantly higher than that of the forth method. However, alternation of primers and adhesive resins produced no significant difference in bond strength related to the adhesive resin, which implies a possible clinical use. 2010 (unpublished). 480 IDENTIFICATION OF PREVOTELLA BARONIAE BY POLYMERASE CHAIN REACTION (PCR) Saengusa Khemaleelakul and Prakaimuk Saraithong Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The purpose of this study was to develop a method for rapid identification of Prevotella baroniae. A pair of specific primers was designed and tested by polymerase chain reactions (PCR). The sensitivity of the PCR was 0.75 picogram. The primers were specific for P. baroniae only. The specific fragment of approximately 848-bp was amplified from both standard strain and clinical strains. The sequences of amplified products were analyzed and found similar to those of P. baroniae. Digestion of the amplified products with a restriction enzyme generated a specific pattern when separated by gel electrophoresis. Our results show that the assay is useful for rapid identification of P. baroniae. 2010 (unpublished). 481 RESONANCE FREQUENCY ANALYSIS OF ORTHODONTIC MINISCREWS SUBJECTED TO IMMEDIATE OR DELAYED LOADING Boonsiva Suzuki and Eduardo Yugo Suzuki Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Purpose: Resonance frequency analysis (RFA) is a reliable, noninvasive method of measuring dental implant stability in vivo. However, the possibility of using this method to assess the stability of miniscrew implant has not been investigated. The purpose of this study was to evaluate the possibility of using RFA to assess the stability of miniscrew implants placed in different sites in the maxillae and mandibles of cadavers. Materials and Methods: Ten pairs of dentate maxillas and mandibles were retrieved from human cadavers. Two hundred self-drilling miniscrew implants of 1.5-mm diameter and 8-mm length were systematically inserted into the dentoalveolar bones aided by a 3-dimensional surgical guide. Maximum insertion torque value was recorded during implantation procedures. Assessment of primary stability was carried out immediately after implantation using the RFA method, and implant stability quotient (ISQ) values were recorded. Analysis of variance, Student’s t test, and generalized estimating equation regression analyses were used to analyze the data. Results: A strong correlation was found between maximum insertion torque and ISQ values (r _ 0.873). Maximum insertion torque and ISQ values were significantly higher in the mandible (15.8 _ 3.3 N-cm and 71.7 _ 12.1) than in the maxilla (11.8 _ 3.8 N-cm and 56.0 _ 15.8). Miniscrews inserted in the posterior region of the maxilla exhibited the lowest ISQ values. No significant difference among ISQ values of miniscrews inserted in the anterior, middle, or posterior regions of the mandible was observed. Conclusions: Estimates of implant stability using resonance frequency analysis are highly correlated with maximum insertion torque. Further studies to assess the primary stability of miniscrew implants in the clinical situation are necessary. 2010 (unpublished). 482 EFFECTS OF USING AN INTERNET-BASED PROGRAM TO PROMOTE HEALTHY EATING BEHAVIOR AMONG THAI EARLY ADOLESCENTS Nongkran Viseskul1, Natthawan Suwan1 and Latthawat Sonsrinusorn2 1 Faculty of Nursing, Chiang Mai University, Chiang Mai 50200, Thailand. The Prince Royal’s College, Chiang Mai 50000, Thailand. 2 ABSTRACT Thailand is similar to many other countries where unhealthy eating behavior among adolescents is an important contributor to nutritional problems among youth. Meanwhile, innovative and effective strategies are needed to promote healthy eating behavior among adolescents. The objective of this quasi-experimental research was to determine the effects of an Internet-based program for promoting healthy eating behavior among Thai early adolescents on knowledge of healthy eating, attitudes towards healthy eating, eating behavior. The study sample consisted of 76 students who studied in grade 7 at a private school in an urban area of Chiang Mai. They were randomly assigned into experimental group and control group, 38 in each group. The experimental group used the Internet-based program during 12 weeks whereas the control group received only the normal lecture in the class and other health promoting activities in the school. This study extended over a period of eleven months from March 2010 to January 2011. The instruments consisted of the test of knowledge in adolescence’s food consumption, the attitude to food consumption questionnaire, the food consumption behavior questionnaire, and the Internet-based program satisfaction questionnaire. Data were analyzed by descriptive statistics and t-test. The findings of this study revealed that: 1. The mean scores of knowledge of food consumption, attitude towards food consumption, and eating behavior among early adolescents after implementing were significantly higher than that before implementing, at the .05 level. 2. After implementing, the mean scores of knowledge of food consumption, attitude towards food consumption, and eating behavior among early adolescents in the experimental group were significantly higher than the control group, at the .05 level. The research findings indicate that the Internet-based program was effective in improving knowledge of food consumption, attitude towards food consumption, and eating behavior among early adolescents. Therefore, the Internet-based program should be applied to promote healthy eating behavior among early adolescents and always updated. Supported by Thai Health Promotion Foundation (ThaiHealth), February 2011. 483 LESSON LEARNED FROM CHILDCARE CENTER MODEL IN FACULTY OF NURSING, CHIANG MAI UNIVERSITY Pimpaporn Klunklin1, Somchit Kiatwattanacharoen1, Nethong Namprom1, Patcharee Woragidpoonpol1, Jutamas Ponmark2, Sureeporn Suwanna-osod3 and Pradchayaporn Thisar2 1 Faculty of Nursing, Chiang Mai University, Chiang Mai 50200, Thailand. School of Nursing University of Phayao, Phayao 56000, Thailand. 3 Faculty of Nursing, Naresuan University, Phitsanulok 65000, Thailand. 2 ABSTRACT The lesson learned from the Child Study Center Project, Faculty of Nursing, Chiang Mai University, was a part of the whole study of “the lesson learned from child care center pilot program” under the supervision of nursing academic institutions in Thailand. The objective of this study was to review or summarize past experiences in various aspects learned from conducting child care under the Child Study Center Project so as to reflect all details of internal and external factors, either successful or unsuccessful. The participants were 58 children, 24 guardians, 16 child caretakers, and two administrators of the Child Study Center Project, in order to have a general view of the system framework of the input-process-output (IPO) model as well as practice guidelines successfully implemented, and guidelines to problem solving. This descriptive research was undertaken using both quantitative and qualitative data collection methods. The quantitative data was analyzed by using number, percentage, and standard deviation; the qualitative data, content analysis. The successful input factors of the Child Study Center Project, were the academic committee from the faculty members of the Department of Pediatric Nursing, a nurse as a project head who could run the project and perform the task independently, together with child care workers having more than 10 years of child care experience, and financial support from the Faculty of Nursing. Additionally, sets of child care plan and child health promotion guidelines were implemented in the center Regarding the process, quality control was performed by a child care team from internal and external agencies under Child Care Center Quality Assessment by conducting an ongoing research and using the research results to develop child care activities, and integrating nursing knowledge into practice for 484 the project operation by having nursing students train at or make study visit to the center. The outcomes revealed that not only the children had benefited from child health promotion practices but also the guardians, and the child care workers expressed high levels of satisfaction. Many child care plans and research projects were developed and emerged continuously. Furthermore, a large number of study visits were from various national and international academic and commercial institutions. This lesson learned from the Child Study Center Project, Faculty of Nursing, Chiang Mai University indicated operational guidelines for child health promotion in child care center which can be the best model for developing more child care centers in nursing academic institutions. Supported by Thai Health Promotion Foundation (ThaiHealth). 485 NORMALCY FROM THE PERSPECTIVES OF CHRONICALLY ILL CHILDREN, FAMILIES, AND NURSING PERSONNEL Kasara Sripichyakan and Jutamas Chotibang Faculty of Nursing, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Normalcy is a concept describing illness in a holistic aspect. However, there is no clear body of knowledge in this concept. This qualitative study aimed to understand normalcy of chronically ill children and families. Research participants included 1) 22 children with cancer or blood diseases (age = 6-14 years, average = 11), 2) 24 parents or child caretakers (1= older brother), 3) 12 all-level-nursing personnel of Pediatric Unit 5, Maharach NaKorn Chiang Mai Hospital, 4) 30 forth year undergraduate nursing students, and 5) 5 nursing instructors. Data were collected through interviews and focus group interviews, as well as drawing or essay-writing in some children. Content analysis was employed to analyze the obtained data. It was found that normalcy of chronically ill children and families was shown from being happy despite of child illness by simultaneously obtaining therapy and living as usual, as needed, or like others, to some extents in spite of not wholeness. Abnormalcy of ill children included 5 dimensions as follows: 1) psychoemotion: irritable, fussy, stressful, quiet, depressed, bored, lonely, homesick, forced, changed body image, concerned with parents’ well-being, scared of dead children, 2) physical health: weak, easily tried, slim, easily infected, discomfort, and painful, 3) decrease intelligence, thinking, and memory, childlike behavior, 4) activities: no schooling, no friends, no playing, no going out to have fun, and limited areas, and 5) daily living: unfamiliarity, inconvenience, non-tasty food, lost privacy, safety concern, and travelling problems. Abnormalcy of families included 7 dimensions: 1) psychoemtion: suffering, passion, fatigue, helplessness, uncertainty, denial, and consideration, 2) physical health: tiredness, rest deprivation, increased illness severity, 3) lost or changed working, decrease incomes, 4) difficulty in caring ill children: spoiling versus being rigid, overload caring, 5) caring of healthy children incompletely, health children’s feeling of inferiority, 6) relationships with spouses: conflicts, disputes, divorce, and 7) daily living: decreased usual activities, decreased self-care, inadequate rest, and inconvenience. Achievement of normalcy from children’s and families’ perspectives included 1) child healing by health personnel, parents, and children, 2) balancing between therapy and child life: middle ways and understanding child life, 3) living normally with changes through doing 486 as usual, as needed, or like others, but modifying or reducing, accepting changes until being familiar with them, being optimistic, relieving suffering, and taking self care of the parents, 4) making a child ‘having funs,’ whereas the parents feeling ‘going to hospital not for funs,’ and 5) obtaining helps. From the nursing personnel’s perspectives, normalcy could be obtained through 1) curing a child, 2) balancing between therapy and living normally: allowing sometimes and understanding child-parent life, 3) living normally with changes through allowing to do as usual, as needed, or like others, reminding or monitoring when unforbidden, 4) giving ‘happiness’ to children, families, (and also personnel) through some activities, such as giving a little gift, organizing play, proving student classes, making handicrafts to earn some money, etc., by the following strategies: do by heart, integrate with usual activities, easy, self and other cooperation, good management, and 5) treating children and families as if own families and living at home through closeness, decorating like a home, reliving suffering, no discrimination, mourning for the deceased, being patient, and helping about financial problems. It is suggested that nursing personnel take care of chronically ill children and their families by balancing between therapy and living a normal life as usual or like others, as much as possible. Supported by Faculty of Nursing, Chiang Mai University. 487 ROLE MODEL BEHAVIORS OF NURSING FACULTY MEMBERS IN THAILAND Areewan Klunklin1, Piyawan Sawasdisingha1, Nongkran Viseskul1, Naomi Funashima2, Tomomi Kameoka3, Yuriko Nomoto4 and Toshiko Nakayama2 1 Faculty of Nursing, Chiang Mai University, Chiang Mai 50200, Thailand. School of Nursing, Chiba University, Chiba, Japan. 3 Department of Nursing Education, National College of Nursing, Tokyo, Japan 4 Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Ehime, Japan. 2 ABSTRACT Being a role model is very important in order for nurse teachers to promote students’ competence and confidence. This descriptive study aimed at exploring the role model behavior of nursing faculty members in Thailand. The Self-Evaluation Scale on Role Model Behaviors for Nursing Faculty (Thai version) was used to collect data from 320 nursing faculty members in eight schools of nursing, four university nursing schools, one college under the Ministry of Public Health, one under the Bangkok Metropolitan Administration, and two private schools of nursing. The results revealed that the mean score of the overall items in the role model behaviors of nursing faculty members in Thailand, as perceived by themselves, was at a high level. The scores on each subscale of the role model behaviors also were high and related to respect for students, enthusiastic and high-quality teaching activities, showing the value of nursing practice and the nursing profession, social appropriateness, and ongoing professional development. The results can be used to further develop nurse professionals and to improve the effectiveness of clinical teaching in Thailand. Supported by Faculty of Nursing, Chiang Mai University. 488 THAI NURSING STUDENTS’ ADAPTION TO PROBLEM-BASED LEARNING: A QUALITATIVE STUDY Areewan Klunklin1, Pornpun Subpaiboongid1, Pongsri Keitlertnapha1, Nongkran Viseskul1 and Sue Turale2 1 Faculty of Nursing, Chiang Mai University, Chiang Mai 50200, Thailand. Faculty of Health Sciences, Yamaguchi University, Ube, Japan. 2 ABSTRACT Student-centred forms of learning have gained favour internationally over the last few decades including problem based learning, an approach now incorporated in medicine, nursing and other disciplines’ education in many countries. However, it is still new in Thailand and being piloted to try to offset traditional forms of didactic, teacher-centred forms of teaching. In this qualitative study, 25 undergraduate nursing students in northern Thailand were interviewed about their experiences with problem-based learning in a health promotion subject. Content analysis was used to interrogate interview data, which revealed four categories: adapting, seeking assistance, self-development, and thinking process development. Initially participants had mixed emotions of confusion, negativity or boredom in the adaption process, but expressed satisfaction with creativity in learning, group work, and leadership development. They described increased abilities to problem solve and think critically, but struggled to develop questioning behaviours in learning. Socio-culturally in Thai education, students have great respect for teachers, but rarely question or challenge them or their learning. We conclude that problembased learning has great potential in Thai nursing education, but educators and systems need to systematically prepare appropriate learning environments, their staff and students, to incorporate this within curricula. Supported by Faculty of Nursing, Chiang Mai University. 489 WOMAN SEXUAL RIGHTS AS PERCEIVED BY YOUNG ADULT MEN IN CHIANG MAI, THAILAND Kasara Sripichyakan and Apirach Indarangkura Na Ayuthaya Faculty of Nursing, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This study identified relationships between men’s perception on woman sexual rights (MPWSR) and general, reproductive, and relationship characteristics of 297 sexually active men (average age = 30.8), through Chi-square test and logistic regression. Significantly (p<.05, Nagelkerke R2 = 0.367, overall statistic = 71.4%), low MPWSR was found among men who were characterized as: young, short period of Chiang Mai residence (OR=2.24), rural lifestyle, less frequent drinking, negative gender equality attitude (equal ability OR=0.54, equal decisionmaking OR=3.21), low sex education (OR=3.59), low woman number in sexual relationships (OR=1.95), and visiting commercial sex workers (0.32). Considering intimate relationships, low MPWSR were associated with short relationship period, relationship dissatisfaction (OR=3.21), sexual dissatisfaction, scarce contraception use (OR=2.78), physical or sexual abuse perpetration, and physical or sexual abuse victimization. Education about woman sexual rights should be provided to at risk men, leading to the promotion of men’s and women’s sexual health. Supported by Faculty of Nursing, Chiang Mai University. 490 Architecture Building Facilities Management Piyaboon Nilkaew, Ekkachai Mahaek and Surachai Singhtoraj Faculty of Architecture, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The principal objective of building management is to control facilities planning and facilities operations and maintenance, that effective criteria in strategic planning about preventive maintenance and predictive maintenance. The key of success in manage the building and facilities is all about collecting and interpreting data on diverse facets of property use. Computer databases are the ideal vehicles in which to log, store and manipulate data; almost unlimited information can be measured and entered en masse. The strength of such information storage is its capacity for expansion and the diversity of subject; it becomes large and requires greater and greater operator familiarity with its structure in order to interrogate successfully. The ultimate solution is to computer-base the entire operation, by using the three-dimensional building modeling to control the operation. This solution will simulate building in virtual environment and the building system data (Architectural part and Engineering part) will collect in digital data type. The digital data will classification and made three - dimensional database relations. This research focus in three section of the operation as three - dimensional database relationship, topological simulation and smart system, that applied to generate the prototype building management application “Architecture Building Facilities Management: ABFM”. Supported by the Thailand Research Fund. 491 Performance of Chimney Techniques: Case of Chiang Mai House Yuttana Tongtuam, Ekkachai Mahaek and Sethapong Sethabouppha Faculty of Architecture, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Design using natural ventilation techniques is one choice that helps people feel comfortable more than. From the influence of wind speed accelerate the evaporation sweat and rancid moisture. But some area can’t get natural ventilation because around the building is near the traffic, air pollution and heat or that has Building density. Then it has a little space and distance sufficient to result from the flow of wind. The objectives of this research are study the potential design of chimney for Thailand in case of Chiang Mai house. And provide guidelines for chimney design for one storey, two storeys and more than two storeys of the house. Those are study for chimney technique that not considers the influence of wind outside the building. This study used weather data of Thailand and used bio climatic chart to compared and evaluated human comfort. This study analyzed data by math model. Consideration compared real condition from operating building for test the possibility. The results showed that height mass building is the best case and low mass building is the worst case for get human comfort compare with outside climate building. Because height mass building affect very different temperatures forward low mass building. When chimney is higher affect speed of wind or ventilation rate is increasing in rate decreased follow from Chimney height. Height mass building has clearly Differences more than low mass building. Ventilation rate in building is 7 times of volume inside enough when design height mass and low mass building. And mass of building affects to decreases heat from outside building (Not hot or cold) so feeling to human comfort more than outside climate. So considered effective of chimney must to has two relative parameters are height and mass of building. Other parameter is area of opening affects to increases ventilation rate follow from area and ratio of inlet and outlet opening. And the best design building in rural area of Chiang Mai is height mass building for every height distance of chimney then felling comfort. Supported by the Chiang Mai University Fund. 492 Application of Lean Thinking for Waste Reduction in Hard Disk Drive Manufacturing Sakulrat Kunyatep, Songkran Panthurat and Rungchart Chompoo-inwai Department of Industrial Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract Hard disk drive (HDD) manufacturing is one of the industries that are highly competitive. Although demand for HDD in mobile and consumer applications is still growing, to survive in the current global economic recession, HDD manufacturing companies have to reduce their cost to the lowest level. In addition, the HDD business is changing all the time due to continuous technological development. The survivors must provide customers good quality, with low cost and with shorter lead time. The objective of this research is to eliminate wastes in the HDD manufacturing process using Lean concepts with the ultimate goal of shortening the production lead time. The studied was carried out in one of the HDD manufacturing companies in Thailand. The study began with the construction of the present and future states of value stream mapping (VSM) to identify waste and its sources. Related activities were analyzed and classified into two groups: value added and non-value added activities. Lean thinking techniques such as Lot Size Reduction and Small Lot-Conveyance, and ECRS were proposed to eliminate non-value added activities, as well as wastes in the process. Related data were obtained from the manufacturing plant, and an Arena simulation model was developed to stimulate the proposed approach. The results indicate that, by applying Lean principles, the case study company’s production efficiency could be improved. Three main wastes in the process consist of waiting, inappropriate processing, and unnecessary or excess motion could be decreased from 75.35% to 58.22%. Production lead time could be reduced from 49 hours, 27 minutes to 40 hours, 59 minutes or about 17.13% Key words: Lean Manufacturing, Waste Elimination, Hard Disk Drive, Production Lead Time 2010 (unpublished). 493 Continuous Combined Infrared and Hot Air Dryer for Laboratory Scale Kittichai Sucomma and Kodkwan Namsanguan Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. Abstract The objectives of this research were to develop and test a laboratory scale, continuous combined infrared and hot air dryer for drying whole longan. The dryer consisted of a 0.60 x 0.60 x 1.50 m3 drying chamber having the product capacity of 15 kg of longan, two components of heat equipments i.e. a 5 kW infrared heater controlled by adjusting the electric voltage and a 15 kW electric air heater, a 0.37 kW centrifugal fan for forcing air into dryer, and a 1.5 kW electric motor for driving the belt conveyer. The flow rate of product was 5 kg/hr. For preliminary tests, dryer was firstly operated without product at air velocities of 0.5-2.0 m/s to study the velocity distribution inside the drying chamber and at drying temperatures of 40-80°C, velocities 0.5-1.5 m/s and infrared power of 1.5-3.5 kW to study the temperature distribution. Drying experiments were then performed on a dryer at drying temperatures of 40 and 80°C, an air flow rate of 1.0 m/s and an infrared power of 2.5 kW to evaluate the dryer performance. Longan with the initial moisture content of approximately 240-280% db. was dried until the final moisture content of around 18% db. Key words: Conveyer/ Dryer/ Hot air/Infrared/Longan 2010 (unpublished). 494 DATABASE FOR LAND USE PLANNING IN CHANGPUAK MUNICIPALITY Sudarat Auttarat1 and Sarawut Pongleerat2 1 Social Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Geo-Informatics, The Far Eastern University, Chiang Mai 50100, Thailand. 2 ABSTRACT Changpuak municipality is the affected area from Chiang Mai city expansion and also relate to major educational institutes cause perpetually more density of residential and commercial activities in this area that initiate rapid land-use transformation without proper urban planning especially in disperse of residential land-use increase number of dormitory, condominium, urban village, etc. These type of improper land-use create problems to overall environment and perspective. This causes led the research to produce database from the study and basic analysis in physical, economic, social, demographic and city labor in consist of survey in current problems in the area to support prospective of urban planning and/or urban design in more proper land-use. The Result of this research obtained from field survey and questionnaire showed that the problems in Changpuak municipality must be resolve urgently has 5 problems; That is flooding and drainage, traffic jams, acoustic pollution, car parking obstruct the traffic flow and high rise building. From the thoroughly analysis found the major cause of problems came from increasing of dormitory, condominium and mansion. Some of these building construct incorrect from the applicant and violated building regulation or even alteration of building without permission and also building owner thrust the burdens to the public such as lack of parking lot in their building cause resident to park their cars in public streets which affect the flow of traffic in street and consist of widen of the street that was not planned for accommodate high volume of traffic. The constructions also impede the flow of drainage resulting in flooding in some area. Therefore it has additional problems such as wastewater and sewage management, Unstable of natural and built environment balance, shortage of water supply, pollutions, rapidly amount of wastes increasing, etc. From the analysis, the mentioned problems indicated that most of the problem derive from improper land-use planning and also affect more problematic conflict between local habitats and entrepreneurs. These problems need a solutions that led by local government agency to provide and declare the proper land-use planning that arise from intrinsic area potentials and public participation to reduce those land-use contradiction. 2010 (unpublished). 495 The Knowledge Management in Lan Na Traditional Medicine: A Case Study of Mareng (Baheng) Illness. Panpen Kruathai1, Nitaya Boontim2 and Amornrath Feungworatham3 1,3 Social Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 2 ABSTRACT Based on the study of 34 Lan Na manuscripts on the traditional medicine, the project titled, “The Knowledge Management in Lan Na Traditional Medicine: A Case Study of Mareng (Baheng) Illness,” has made the 2 conclusions about mareng (‘baheng’ in the Northern Thai dialect). It was divided into 2 groups. First, Mareng (Baheng) which means a disease having symptoms similar to skin disease such as itching, rash, boil, saan. It is not a severe disease, e.g. mareng khai pla, mareng fa-mahn, mareng fai. Second, Mareng Kut (Lom baheng kut) is a disease encompassing all symptoms of the wind element and pain or other symptoms that occur on different spots on the body such as mareng kut headache, mareng kut which appears on the eye, mareng kut sinnibaht klang lai. These kinds of mareng were curable. The mareng therapy included the use of medicinal plants in conjunction with chanting magical formulas and performing a ritual according to traditional belief. As for the use of herbs, it was found that of the 300 tam rab, 155 were used for curing mareng; and of 368 tamrab, 140 were used for curing mareng kut. However, there are 19 types of herbs that are endowed with properties for curing the diseases in the mareng group. We have noted the frequency of herbs mentioned in the aforesaid medicinal texts. Ranking from high to low, we have found the following: khao chao (200), thua poo (130), phuk waan (91), pung dau (71), rang yen (64), ngaa chang (60), phuk diid (50), luem dam (46), joi nang (45), huan moo (41), lab muen luang (41), phuk khao (40), phuk khab (38), jii puk (34), nam man ngaa (33), lom lang (27), khi lek (27), pid piew daeng (26), and khao min (25). The above-mentioned herbs are medicinally important. Thus, related studies should be made of them in pharmaceutical sense. Also, they should be widely cultivated for use in health care, marketing, and sustainable development. 2010 (unpublished). 496 URBAN PLANNING PRACTICE TO SUPPORT CHIANG MAI CITY RENEWAL Duongchan Charoenmuang1, Pranom Tansukanun2, Isara Guntang3, Nantaka Nimmanwattana4, Kanokwan Kotchasee3, Wittaya Daungthima2 and Sudarat Auttarat1 1 Social Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. Faculty of Architecture and Environmental Design, Maejo University, Chiang Mai 50290, Thailand. 3 Faculty of Art and Architecture, Rajamangala University of Technology Lanna, Chiang Mai 50200, Thailand. 4 Independence Researcher. 2 ABSTRACT To provide well-being to public is one of the purpose for good urban plan which led urban planners must focus on people more than building in city; thus this research conformed the concept of healthy city to Chiang Mai city which is urban plan for the people by public role that determined the content and support the discourse that the city will be more liveliness if it have well planned consist of the citizen live in good health and well-being activities. To drive this sustainable urban development idea process with the participation of all sectors. City’s problem and potential was analyzed in this research to distribute concept in city level, district level and local area level. The result indicated that Chiang Mai city have potential in many ways which are 1) Good physical shape of city; Settle on intermountain plain between Suthep hill (DoiSuthep) on the west and Ping river on the east which making Chiang Mai in good water flow that have natural water source and drainage, respectively. 2) Wat phra that DoiSuthep (phra that DoiSuthep temple) the most valuable spiritual meaning and religious place in Chiang Mai either symbolical, mental and settlement-historical values. 3) DoiSuthep (Suthep hill) which is important cognitive meaning element of landmark. 4) Chiang Mai is historic old town that still have dense residential area. It’s an advantage in the way of making livable city; Conform with the healthy city concept. The good historic city must have well-being city life and adjacent of activities. In addition there are problems such as lack of green areas, traffic, pedestrian continuity problem, lack of parking space, high rise building and etc. The analysis of potential and problems led healthy city urban development guideline that provide in three level of target areas which are Chiang Mai municipal city area, Srivichai sub-district area and Suandok - Nimmanhaeminda local areas. According from public forums that cast vote (Selection of target area) and comments derive from governing, private and public sector in each level to guideline the well-being urban development in Chiang Mai healthy city in the future. 2010 (unpublished). 497 Drawing creation of human forms inspired by line colour Form and texture in ceramic art Pakit Bunsut Sculpture Division, Department of Printmaking, Painting and Sculpture, Faculty of Fine Arts Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Drawing is one part of the roughly way to express the idea of an artists before they start to create their own art works. Normally most artists chose a drawing technique to find out their own art style and process to create a real piece so drawing seems as the original idea of visual art such as painting, sculpture and printmaking etc. The drawing may tell the viewers about the emotion and all kinds of people’s feeling including the previous happened stories in the past. Artists normally transfer their own idea into their hands with some movements by using an object around them as a piece of wood, stone, charcoal, soil to memorize and express their imagination. With my own sculpture art experiences cooperated with a ceramic technique. I have a great inspiration to create my own style of drawing. It represents mainly about my imagination of human’s story by using the element as color, form and texture. All of my art pieces express the feeling of pressure, sadness and suffered combined with human’s actions by using clay as a media to transfer all of these idea into the drawing of ceramic art, including the previous ceramic technique to create this project higher value and aesthetics. 2010 (unpublished). 498 Hill Tribes; Beliefs and Ways of Life towards the Creation of Contemporary Sculpture Soontorn Suwanhem Sculpture Division, Department of Printmaking, Painting and Sculpture, Faculty of Fine Arts Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This research titled “Hill Tribes; Beliefs and Ways of Life towards the Creation of Contemporary Sculpture” presents a specific individual view on beauty through a combination of art, culture, customs, and local traditions of people living in the lowlands with the beauty of the ways of life, traditions, arts and culture of the hill tribe people. The hill tribes’ ways of life incorporates values and a philosophy of self-sufficiency in accordance with the circle of life that was founded by the ancestors which should be preserved for the future generations so that they can absorb the civilization of their different tribes. This may be difficult for us, people from the lowlands, to understand. What can still be seen is a residue from a culture that has cultivated pure spirit and has spread towards every corner of life in that society. No matter where, the ways of life, the roots, the beauty, the love, still exist. Life’s aim is to live together in harmony with the world. There are many thinkers, writers, from the humanities, the social sciences, etc...who have taken this as the basic concept for their work. This also goes for the researcher. I use the knowledge I have on sculpture, search for a certain identity, symbols representing beauty, style, form, technique, materials structural method, using the concept as above, and, through my imagination, create and describe certain forms from my dreams, create mysterious images, communicate topics that reflect the soul and identity of local people from the lowlands and the highlands in harmony. I use the language of sculpture, which, even though there may be some confusion concerning information, style, technique and method, the structure of the work reveals the wisdom of the villager. I find it profoundly valuable for the younger generations that they should learn about the cleverness of their Thai ancestors.The researcher has investigated the content of the research “Hill Tribes’ Beliefs and Ways of Life towards the Creation of Contemporary Sculpture” using five chapters. The first chapter deals with the importance, origins and the 499 problems encountered in this research. The second chapter deals with topics on creativity. The third chapter explains the method and proceedings used to create the work. The fourth chapter contains an analysis of the creative work. The fifth topic is a summary of the research. Furthermore, this research, namely a work of sculpture, has been presented to the public at different locations both in Thailand and abroad. This information is gathered in appendix 1 and 2 which completes the research process. The chapters as described above are only topics. For those who are interested, the detailed content must be read and studied. They may get inspired by the content, the style, form, technique, materials, or the different methods used in the creation of this work of sculpture. 2010 (unpublished). 500 SUSTAINABLE EFFICACIOUS THE RENOVATION OF HUMAN MIND Peerapong Doungkaew Sculpture Division, Department of Printmaking, Painting and Sculpture, Faculty of Fine Arts Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT Modern society, especially in big cities where economies continue to progress at a rapid pace, is home to people who have migrated from many different areas, and do not have a close relationship to each other. Instead, they compete with each other in order to gain benefits, leading to stress and sometimes mental illness, as often they live unhappily, and so turn to drugs and violence instead, ruining their own lives, the lives of their families, and eventually the economy and society. In contrast, in rural or agricultural society, the social environment covers paddy fields, rivers, canals, schools, clinics, fresh markets and temples, where people can carry out religious activities. They have a tight-knit relationship, as they see each other every day when they go shopping at nearby of a community, other will help and send information throughout the rest of the community. This kind of society is called and ‘open society’ Open societies can easily be influenced by outside forces and civilizations, particularly if people in such a society do not fully understand the roots of their culture, do not adapt themselves to their changing social environment while still preserving their own identity, of if they do not properly adjust themselves to a dynamic society. The author lives close by an agricultural society, and has been impressed by the closeness shown among people living there, as if they were relatives. Moreover, there people live their lives in harmony with nature and the environment, exploiting resources only as necessary, and live at a slow pace through their agricultural work, based on the philosophies of a sufficiency economy, sharing among, and maintaining a positive mental health. Impressed by the way of life in agricultural society, the author reflects this feeling through the creation of a sculpture that represents love and care, the relationship among humans and the relationship between humans and nature, plus portrays attractive cultural characteristics, and an appropriate adaptation to modern society. This creative research is divided into six chapters, these being : chapter one, which includes the background to and purpose of the research 501 and the research methodology; chapter two, which provides and explanation of the concepts and inspiration for the work, and the includes an analysis of the artistic visual elements; chapter five, which includes an explanation of the reasons behind the selection of the materials and the creation of the work, and chapter six, which concludes on the research required for such an artistic endeavor. 2010 (unpublished). 502 Ta-Lung of Modern Technology Sugree Gasorntgatsara Sculpture Division, Department of Printmaking, Painting and Sculpture, Faculty of Fine Arts Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The creation of an art piece based on traditions from the Southern part of Thailand fused with modern day technology, especially the computer. The uniqueness of the piece lies in the presentation of the story through crafted leather sheet characters that are animated from behind a screen that separates them from the audience, a light source is shone from the back casting the shadows of the characters onto the screen. The story that is narrated through the movement of shadows, instead of being viewed from a screen, is viewed instead through the computer screen. This form of art is termed mixed media art, utilizing materials including rubber, computer screen, wood, light bulb, for instance. This piece is named Ta-Lung of Modern Technology. 2010 (unpublished). 503 Transforming Chiang Mai: Creative Photography by Plastic Camera Holga Assada Porananond Photographic Art Division , Media Arts and Design Department, Faculty of Fine Arts, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The objective of this creative research is to describe the working procedure of photographic project “Transforming Chiangmai : Creative Photographic by Plastic Camera Holga” in 3 sections, which are Conceptual Process, Character of the Camera and Production Stage. It also tends to analyze problems, decisions, and reasons of the working process. The topics of this creative research come from the idea of transforming Chiangmai city by integrating analog process with digital process to create City-ness that researcher interpreted into conceptual framework. 2010 (unpublished). 504 Laws and Custom in the Management of Natural Resources: Comparative Case Studies of the Karen in Mae Wang District and the Community Forests Management of Chiang Dao District, Chiang Mai Province Boochoo Na Pomphet Faculty of Law, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This project compares Case Studies of the Karen in Mae Wang District and the Community Forests Management of Chiang Dao District ,Chiang Mai Province. The results of this study are taken into account when making suggestions for the improvement of legal community right affirmation in Thailand. The study results showed that, in foreign countries, the community right had been legalized in both international and domestic laws, and also in court judgments. This legalization system played important roles in the affirmation of community right based on norms and traditions of the community. In Thailand, although the constitution B.E. 2540 and B.E. 2550 stated the affirmation of community right, there were no laws issued to confirm the right. For example, the community forest law was still not issued. The court judgments according to the written laws and other considerations of technical problems did not involve community rights at all. These practices caused the problems of community right affirmation in Thailand which cannot be further developed. Therefore, the affirmation of community right in Thailand must be processed under some extended laws. The scope of community right affirmation and the court judgment must take norms and traditions of the community into consideration. They must not be too restricted by written laws and technical problems. This project is an example of Laws and Custom in the Management of Natural Resources that will help support the legal community right affirmation and provoke thoughts or concepts for the further development of the issue. 2010 (unpublished). 505 Project on Empowering Community to Combat Smog Crisis: A Research Study for Alternative Solutions to Smog Problems and Watching Network Development Pairat Trakarnsirinont Faculty of Political Science and Public Administration, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT The project has objectives to specify forest fire and man-made burning area using geographic information system and to propose alternative approaches for local citizen dealing with the smog and fire from agricultural remains following harvest season. The project made policy proposals on roles of government agencies especially local government administration and roles of local citizen to make collaborative efforts and consciousness in combating smoke and fire problem within the research areas. The project held 10 workshops in 12 local communities in Chiangmai, Lam Phun, Lam Pang, and Mae Hong Sorn for the participants concerning processes and approaches to prevent and solve the smog crisis. The 1,114 participants comprised of community leaders (692 persons) and youths (422 persons). Further, the project distributed 10 small and medium size grinding machines to potential communities facing smog crisis. Research fund supported by Chiang Mai Province. 506 Project on Empowering Community to Combat Smog Crisis: Community Development Activities to Build Solution Learning Sites within Communities Pairat Trakarnsirinont Faculty of Political Science and Public Administration, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT The project has objectives to select 2 communities in each 25 Amphor of Chiangmai Province facing high smog risk but potentially cooperating with learning networks for preventing and solving smog crisis. The project held workshops promoting alternative approaches deriving from research that has suitability for the local context in avoiding fire following post-harvest season. In the workshops, participants learned useful knowledge, choices of operations, technology know-how and local collaborative management to deal with the smog crisis sustainably. The workshops began January, 27, 2010 and ended in June, 21, 2010 that made a total of 14 events for 1,580 participants all together. The participants comprised of community leaders, civil officers, citizen, and youth. Further, the project provided 50 revolving funds, called “Community Empowering Fund to Combat Smog Crisis” for 25 Amphor in Chiangmai Province (25,000 baht each). Some potential communities also received small and medium size grinding mechanism (12 machines) to network with the project administration. Research fund supported by Chiang Mai Province. 507 The Changes of Working Procedures and Public Service Delivery Process of Regional Public Organizations in Chiang Mai Province to Comply with the Policy on Citizen-Centered Governance Thanyawat Rattanasak School of Public Administration, Faculty of Political Science and Public Administration, Chiang Mai University, Chiang Mai 50200, Thailand. ABSTRACT This research, “The Changes of Working Procedures and Public Service Delivery Process of Regional Public Organizations in Chiang Mai Province to Comply with the Policy on Citizen-Centered Governance”, aimed to: (1) study the strategic planning development process of the Thai public organizations and its implementation; (2) study factors affecting the citizen-centered governance policy implementation of regional public organizations in Chiang Mai Province; (3) study the changes of working procedures and public service delivery process, paradigm, culture, and value of public officials in response to the citizen-centered governance policy; (4) offer recommendations to the Thai public organizations in order to adjust working procedures, public service delivery and government official’s value; and (5) utilize the research results in the teaching and learning in the course of Public Administration study. Qualitative research methods, including the examination of documentary evidences, the survey research of 37 regional public organizations, and the interviews of 18 key informants, were used in this research. The findings revealed that: 1. Following the governance reform in 2002, the Thai public sector has employed the Result-based Management in its new public administration form. According to this reform, the Thai Government and the public organizations have to formulate their four-year strategic plan as well as annual action plan in order to request for the budget from the central government. In addition, each central public agency has to sign performance pledge that will be used to evaluate its performance in order to get incentive from the Government. Therefore, after the governance reform in 2002, the Thai public agencies have to set up clear goals and indicators in their public administration. 2. After the formulation of a fouryear strategic plan, the central public agency will disseminate strategic issues, goals and indicators as a strategy map to its field offices. Field offices in regional 508 area will implement these strategic issues as programs and projects. 3. Regional public organization in Chiang Mai Province has been responsible for implementing strategic issues set by central public agency. Thus, the regional public organization will have to formulate its annual action plan and adjust its working process and public service delivery with the supports from the central public agency in terms of resources and technology. Furthermore, the regional public organization will improve its working process and public service delivery in order to meet its customer satisfaction since all public agencies will be evaluated their performance using Balance Scorecard in the second dimension (i.e. quality of public service). This improvement includes setting projects to adjust paradigm, culture, and values of its staff in order to improve public service delivery quality. It can be said that this change of public organization has clearer goals than the adjustments in the past. 4. The implementation of the citizen-centered governance project has been in accordance with the four-year strategic plan of the of regional public organization in Chiang Mai Province which will improve public service delivery process and solving people’s problem. 5. Even though human resource is an important factor for public administration and public service delivery; members of staff, especially temporary staff who have been assigned to work closely with customers, did not have the opportunity to attend training program. This was due to the public organizations did not have sufficient budget and regulations for professional development. Research fund supported by Faculty of Political Science and Public Administration, Chiang Mai University, Fiscal Year 2009. 509 The Factors affecting tourist decisions in services selection of Boutique Hotels in Chiang Mai Province Pawini Temdi College of Arts, Media and Technology, Chiang Mai University, Chiang Mai 50200, Thailand ABSTRACT Considerate the factor of traveler who becomes the customer of the boutique hotel in Chiang Mai will produce reliable reference to be the guidance for both traveler who interested in the area of service, and the hotel itself. The research has done by questionnaire which collected from Thai and foreigner guess that stay in boutique hotel in Chiang Mai. With statistical method generated us the indicator of factor which encourage being the hotel’s customer. The research has shows obvious distinguishing of guess that prefer to stay in boutique hotel in Chiang Mai. The majority of traveler guess in the hotel expected unique style of hotel decoration, which is an influence from Northern Thailand couture. The product of boutique hotel and aspect are the foremost factor what convincing traveler to come to the hotel. The hotel service which provide the best service always be appreciated from customer, when can understand what customer want and achieve to serve all requests. These are other factors that produce the positive result of the hotel business. To be the reasonable price is most reverent concern for the hotel, boutique hotel should not be exceeding twice price of service than regular hotel style. Promotion package including long-term stay plan would be eligible for gain reservation demand for the business. The hotel necessitates using CRM (Customer Relationship Management) to maintain customer contentment, and inspire them to return again. Not only facilities conventional, cleanness, and truly relaxing environment, but also including well planning public relation is the matter of fact of successfulness of the boutique hotel business. 2010 (unpublished). 510 Index A Aatit Paungmali 5, 6, 9 Abdalla Muna Ali 369 Abhinav Kulkarni 179 Achalapong Jullapong 265 Achara Eksaengsri 13 Adpakpang Kanyaporn 381 Advincula Rigoberto C. 241 Aekarin Inpramoon 120 Afshin J. Ghajar 453 Aggarangsi Pruk 465 Agnes Guerci-Bresler 32 Ajchamon Tummachai 471 Akira Ito 56 Aliakbar Akbarzadeh 155 Amar S. Bhalla 81 Amnauylawjarurn Teerachai 246 Amornlerdpison Doungporn 215 Amornrath Feungworatham 496 Amornthammarong Natchanon 203 Ampica Mangklabruks 3, 4 Ampun Chaitongsri 3, 4 Anak Iamaroon 476 Ananta S. 263, 264, 267, 309, 377 Ananta Supon 221, 244, 261, 279, 334, 379, 403 Anchaya Dutjanuthat 35 Angela Rory 58 Anil Prakash 57 Annette H Sohn 23 Anong Suntranon 3, 4 Anucha Promwangkwa 139, 150 Anuchart Matanasarawoot 35 Anukoon Siripan 415 Anuluck Junkum 29 Anuntalabhochai S. 345 Anuntalabhochai Somboon 331 Anyarat Wongkhot 417 Apichart Chotchuen 110 Apinun Aramrattana 61 Apirach Indarangkura Na Ayuthaya 490 Apisit Sancom 122 Arakawa Osamu 392 Aramrattana A. 50 Aranya Manosroi 65, 66, 68, 69, 70, 73, 76, 82 Aree Achariyaviriya 138, 146, 162, 186, 447 Areewan Klunklin 488, 489 Ariyadej Surabon 265 Arnold Ganser 32 Artit Yawootti 104, 128 Asawinee Danpinid 158 Asit Apornsupavit 106 Assada Porananond 504 Atchariya Jitpakdi 29 Athipong Ngamjarurojana 71, 75, 81 Atipoang Nuntaphan 177, 197 Atiporn Saeung 20, 46 Auragudom Piched 241 Auras R. 254 Autcharaphorn Apiwongngam 420 Aviva Ezra 418 Ayala Paola 278 B Baba Akira Babaranda De Silva Bancha Jiwdet Bancha Sinsuksate Banjongprasert C. Barrett Ben Batchelor David Bechtold Thomas Benchalak Maneeton Benjaporn Chaiwun Benjawan Pitasawat 269, 271, 336 57 459 452 368 396 278 365 33 48 29 511 Benjawan Tuetun 29 Bergey Elizabeth A. 276 Bernard Roubert 32 Bert Brunekreef 17 Bhalla Amar 221, 244, 261, 403 Bhalla Amar S. 390 Bhuddarak Charatpangoon 449, 461 Blom D. A. 232 Boochoo Na Pomphet 505 Boonmalai Apichart 361 Boonruang Chatdanai 283 Boonsiva Suzuki 479, 482 Boonsom Liawruangath 64, 79 Boonsri Kaewkham-ai 456 Boonthum Anuson 311 Boonyawan D. 254, 300, 378 Boosong Kasempitakpong 35 Boriwat Suebkuna 460 Bounchon Soukchaleun 127 Briand Nelly 362 Brown Andrew P. 351 Brown Mike A. 396 Brydson Rik M. D. 351 Budge Giles E. 396 Bunlue Pensri 276 Bunyapaiboonsri Taridaporn 337 Busabongkoch Chawengchao 11 Bussaban Boonsom 335 Butsan T. 355, 374 C Cai Jianrong 185 Carlo Finelli 32 Carroll David L. 241 Carter James L. 218 Catherine Walton 41, 57 Celentano D.D. 50 Chaimanee Veeranan 314 Chaipanich A. 253, 257, 280, 284, 317, 323, 341 Chaipanich Arnon 236, 237, 250, 402, 408, 409 Chairoj Rattanakawin 180 512 Chairote Griangsak 233, 294 Chairuangsri T. 305 Chairuangsri Torranin 200, 210, 258, 351 Chaisiri Angkurawaranon 61 Chaiwat Ngampiyasakul 27 Chaiwat Pumchat 455 Chaiwong C. 254, 300 Chaiyavat Chaiyasut 63 Chakkrawut Taecho 133 Chamchuree Sotthikul 84 Chamnong Kingkeow 3, 4 Chananchida Singkamanee 421 Chandarak S. 260 Chandet Nopakarn 356 Chankaew Chaiyos 307 Chanokporn Chaiwong 93, 96, 102 Chansaenpak Kantapat 397 Chantana Padungtodf 25 Chantaramongkol Porntip 218, 276 Chantara Somporn 322, 365 Chantawannakul Panuwan 314, 396 Charin Ya-In 53 Charuluxananan S. 21 Chatchai Kruea-In 67 Chatchai Muenkonkeaw 3, 4 Chatchawan Chaichana 125, 188 Chatkaew Simarak 415 Chau-in W. 21 Chawasak Rakpenthai 109 Chayanon Hansapinyo 448, 449, 452, 461 Chen Shui-Tein 214 Cheunbarn Siraporn 240 Chimupala Yothin 406 Chindaprasirt Prinya 385 Chirachanchai Suwabun 327 Chisvert Alberto 292 Chitapanarux Imjai 352 Chitapanarux Taned 352 Chitmanat Chanagun 367 Chitsanu Pancharoen 27 Chitsiri Rachtanapun 85 Choeyklin Rattaket Chokchai Wongsinsup Cholamjiak Prasit 211, Cholamjiak Watcharaporn Chomdej S. Chomdej Siriwadee Chomsaeng N. Chontananarth Thapana Choonluchanon Somporn Choopan S. Choopun Supab 273, 274, Chotechuang Panasoponkul Chotivanich Nantasak Christian Rose Christophe Biot Christopher K W Lai Chuchart Pintavirooj Chulabhorn Pruksachatku Chuleeporn Veerakajohnsak Chulin Likasirib Chumpolsri Watcharapong Chuntana Suwanthada Churat Thararux Churdsak Jaikang Chutchawan Tantakitti C. Jeya K. Henry C. Kongnam Collins Intira J. Cressey Tim R. Cuthbertson Andrew G. S. 239, 202, 216, 275, 338 62 411 410 259 213 305 348 332 264 414 56 265 32 76 17 113 55 117 112 389 84 190 63 450 431 105 265 265 396 D Danai Boonyakiat 78 Dankittipakul Pakawin 231, 286, 398 Dany Habr 32 Daothong Suphaporn 278 Dararat Pankan 182 Darin Sosothikul 43 Darin Srisawat 86 Dasgupta Purnendu K. 248 David P Strachan 17 Dechakupt T. 267 Deepak Joshi 46 Dejang N. 281 Dejang Nuchjira 278 Dell Bernard 313 Det Damrongsak 151 Dheerawan Boonyawan 93, 94, 96, 102 Dhompongsa S. 288, 355, 374, 376, 393 Dokmaisrijan Supaporn 327, 394 Dominique Vassilieff 32 Doungrat Riyong 29, 56 D. Singwong 107 Dumrong Jaikeankea 90 Duongchan Charoenmuang 497 Duong Socheat 41, 57 E Eakrit Kaewjaren 457 Edmonds David V. 351 Edmund Capparelli 13 Eduardo Yugo Suzuki 479, 482 Eitsayeam Sukum 245 Eitssayeam S. 262, 315, 343 Eitssayeam Sukum 312 Ekachai Chukeatirote 419 Ekarin Chaiya 181 Ekkachai Mahaek 491, 492 Elisa E. Konofagou 158 Elliott Stephen 282 F Fang Li 404 Fasai Wiwatwongwana 444 Frenkel Lisa M. 265 Friedrich Götz 68, 69, 82 Fupinwong W. 288, 355, 393 G Gabor Domokos Gao Jian Gavivann Veerakul Geng Xiang-Dong German D. Gillian M. 32 405 43 405 50 202 513 Gillian M. Greenway 64 Gi-Sik Min 46 Gobwute Rujijanagul 67, 77 Gonzague Jourdain 39 Gosalawit Rapee 327 Grant P. S. 368 Greenway, Gillian M. 202 Grudpan Kate 199, 203, 248, 290, 291, 360, 361, 380, 388, 412 Grudpan Supara 412 Guo Ruyan 221, 244, 261, 390, 403 H Hai Le Ha 98 Hall Jayne 398 Ham Dong Jin 238, 308, 382 Hao Ya-Juan 405 Hartwell Supaporn Kradtap 290, 291, 360, 361, 388 Haruta M. 305 He Li-Juan 405 Hemvuttiphan Jittapol 265 Hideaki Nakagawa 24, 25 Hideki Sakai 66 Hideyuki Sawada 108 Hinjiranandana Temsiri 362 Hintong T. 21 Hintong Thanoo 59 Hiroyuki Akazaw 65, 76 Hogg S. C. 368 Honarparvar B. 399 Hongsibsong Surat 350 Hongsith N. 218 Hongsith Niyom 274, 358, 414 Hong Suk Joon 382 Hotrawarikarn Somboon 362 H Ross Anderson 17 Hyde Kevin D. 209, 219, 335, 338, 339 I Imnang S. Imurai Suttawan 514 373, 375 210 Ingwersen J. Insom Norkaew Intatha Uraiwan Inthakon W. Isara Guntang Isoda S. Isoda Seiji Itthichai Preechawuttipong 364 123 312, 315 376 497 305 381 169, 463 J Jacek Namiesnik 418 Jaeger Peter 231 Jaidee Kanpicha 387 Jaimasith Manat 200 Jaitanong N. 253, 257, 280, 284, 317, 341 Jaitanong Nittaya 250 Jaita P. 208 Jakmunee Jaroon 248, 291 Jamjai Utan 215 Jamnong Uthaibutra 80, 421, 424 Jampeetong Arunothai 395 Janhom Sorapong 346 Jannoey Panatda 233, 294 Janthawichai K. 30 Jareansuk L. 253 Jarupanich Tapnarong 265 Jarupoom P. 272 Jattukul Siriprapha 391 Jejira Khamsuwan 72 Jenjira Piamdee 179 Jiansirisomboon Sukanda 208, 255, 268, 281, 301, 370, 404 Jianwen Luo 159 Jia Yan 278 Jin Li 298 Jintanat Ananworanich 27, 39 Jiradej Manosroi 65, 66, 68, 69, 70, 73, 76, 82 Jiranan Griffiths 474 Jirasak Panya 144 Jirawat Chiatrakul 147 Jitonnom Jitrayut 316 Jitra Waikagul Jittapa Duangsawat Jittichat Tilawat Jocque Rudy John T. H. Jonathan Vappou Jones Ben Jones E. B. Gareth Jourdain Gonzague Jumpathong Juangjun Jurai Wongsawat Jurmkwan Sangsuwan Jutamas Chotibang Jutamas Ponmark Jutamas Suvimolcharoen Jutharat Boonpanya 56 88 442 286 210 158 396 338 265, 362 369 27 88 486 484 415, 416 132 K Kabkaew L. Sukontason Kaewkhao A. Kaewsai D. Kahl G. Kajornchaiyakul Julatep Kamkaew Anyanee Kamonpan Pengpat Kampuansai Jatupol Kananthai Amnuay Kanchana Siripon Kanchiang K. Kanchiang Kanokwan Kanda Whangchai Kaneko Futao Kanetsuna Yoshinori Kangtunyakarn Atid Kangwanpong Daoroong Kanitha Yajom Kanjanapothi Duangta Kanjana Rueanto Kanjanasing Annop Kanjanavanit Suparat Kannanuch Srikok Kanokporn Niwatananun Kanokwan Kanchiang 34, 36 318, 353 404 364 210 199 67, 77, 101 298 204 265 303, 377 390 421, 424 269, 271, 336 336 371 298 454 215 76 265 365 88 63 71, 81 Kanokwan Kotchasee Kantha Puripat Kantha Uthen Kanyanee Tinakorn Kanyaporn Chaiwong Kaowphong Sulawan Karen Kirkby Karnoksak Loiloes Karn Patanukhom Kasame Thipo Kasara Sripichyakan Kasemsak Uthaichana Kasem Uttawichai Kasinrerk Watchara Katayama Takeshi Kate Grudpan Kato Keizo Katy Morgan Kauppinen Esko Kavinchan Jutarat Kavinchan Nuttira Kazunori Nosaka Kengsakul Karun Kenneth McIntosh Kerdcharoen Teerakiat Kerry Taylor Ketsuwan P. Kettapun Atichart Kevalee Unachak Khajornsak Sopajaree Khaleghian M. Khamman Orawan Khanittha Taneyhill Khomhoi Padungsee Khotchanin Kanyarat Kiat Ruxrungtham Kilburn Jeremy D. Kiran D Pandey Kirk C. A. Kirtsaeng Sukrit Kitipong Nakpakdee Kittichai Sucomma 497 245 465 248 111, 190, 194 220, 227, 256 72 90 157 100 486, 490 108, 130, 455, 456 33 350 233 83 269, 271, 336 41 278 324, 413 282 9 265 13 358 32 264, 267 204 48 122 399 403 469 401 302 27 310 17 368 322 111, 175, 194 494 515 Kittichai Wantanajittikul 170 Kittikhun Moophayak 34, 36 Kittikorn Sasujit 149 Kittinan Noimanee 113 Kittiworawat Saengdao 247 Klin-eam Chakkrid 287, 372 Klysubun W. 325 K. Noimanee 166 Kobkiat Saengnil 421 Kodchakorn Kanchanok 316 Kodchakorn Mahasongkram 51 Kodkwan Namsanguan 162, 447, 494 Kodwan Namsanguan 138 Koetsawang Suporn 265 Komboonchoo Sunsanee 365 Komgrit Leksakul 441 Komkham Pattanaporn 430 Komson Wannasai 26 Kom Sukontason 34, 36 Kongtawelert Prachya 316, 361 Koonaphapdeelert Sirichai 465 Korawinwich Boonpisuttinant 70, 76 Ko Thida Win Ko 339 Kovit Pattanapanyasat 27 Kowit Nambunmee 24 Kreasuwun Jiemjai 246, 322 Krissana Romchaiyaphruk 463 Krit Makonkawkeyoon 38 Krongkarn Pugpayappysal 433 Kruea-In C. 262 Kudumpee Sudkaneung 352 Kuimalee Surasak 351 Kulkanya Chokephaibulkit 13, 23, 39 Kulthida Chaisathidvanich 423 Kumarasamy, Nagalingeswaran 23 Kungwankunakorn Sukjit 321 Kuntalue Budsabong 321 Kurata H. 305 Kusumaphanyo C. 59 Kyung-Ho Park 452 L Laatsch Hartmut Lallemant Marc 516 369 265, 362 Laoratanakul Pitak 293 Laorodphan Nattapol 200 Laosiritaworn 293 Laosiritaworn Y. 303, 309, 377 Laosiritaworn Yongyut 221, 244, 261, 334, 390, 403 Lapanantnoppakhun Somchai 291, 360, 388, 412 Lapmak Kodchakorn 320 Latthawat Sonsrinusorn 483 Le Coeur Sophie 265, 362 Lee Jae Sung 238, 308, 382 Leenakul Wilaiwan 245 Leeratanavalee Sorasak 234 Lee T. Randall 241 Lee Vannajan S. 401 Lee Vannajan Sanghiran 199, 316, 327, 330, 331, 380, 394 Lee V. S. 378, 399 Lefteri H. Tsoukalas 130 Lekprasert V. 21 Lertlakana Bhoopat 26, 53 Liampongsabuddhi Prateung 265 Liawraungrath Saisunee 202, 243, 292, 304, 340, 354, 359 Liawruangath Boonsom 202, 243, 304, 359 Liewhiran Chaikarn 289 Li G. R. 317 Linda Aurpibul 12, 44, 47, 49 Liotti E. 368 Lisa J. Mauer 91 Liu Diansheng 405 Loetchutinat Chatchanok 206 Lomthaisong Khemika 222, 311 Lorvidhaya Vicharn 352 L. P. Purba 172 Lueangprasert Kanyarat 392 Lu Huazhu 185 Lumyong Pipob 313 Lumyong Saisaimorn 209, 219, 233, 270, 294, 297, 313, 320, 335, 337, 338, 339, 369 Lu Qiang 185 M Mana Saedan 131, 137, 178 Manas Sangworasil 113 Manatpong Mongkrai 178 Manee Pinyopornpanish 33 Marasri Chaiworawitkul 143 Mari Pat Toye 13 Marriott Philip J. 235 Marris Gay 396 Mary Elizabeth Smith 13 Masahiko Abe 66 Masahiro Takagi 58 Mathias Schmid 32 Matsuzawa Taihei 271 Matteo Della Porta 32 Matthew Cole 106 Matthew Law 23 Matthew O. T. Cole 168 Maxwell James F. 217, 249 McIntosh Kenneth 265, 362 McKelvie Ian 388 McKenzie Eric H. C. 219 Medapon Bomkao 415 Meepowpan Puttinan 206 Mekchay S. 259 Metee Medhasith Suksumret 415 Mi-Hyun Park 46 Mi J. 368 Milan Suhaj 418 Milena Cvikrova 418 Milne S. J. 342 Moh Seng Chang 57 Mollaamin F. 399 Molwipa Sirihorachai 3, 4 Mom Chandara 27 Monajjemi M. 399 Monnier J. R. 232 Muneko Nishijo 24, 25 Murani Eduard 213 Muthita Trakultivakorn 16 N Nacapricha Duangjai 203 Nagalingeswaran Kumarasamy 23 Nahathai Wongpakaran 33 Nakarin Saengow 181 Nakhorn Thonglek 148 Nakorn Tippayawong 104, 118, 120, 124, 126, 128, 135, 139, 141,151, 153, 161, 169, 171, 174, 181, 195 Namboothiry Manoj A. G. 241 Namsar Orapim 255 Namwong Prachaya 200 Nanjaras B. 242 Nantaka Nimmanwattana 497 Nantana Charoonnate 37 Nantaporn Kumsuk 100 Nantawan Saengroj 198 Naomi Funashima 488 Naraballobh Watcharapong 213 Narawut Srisang 181 Narin Sirikulrat 74 Narinthorn Khositsuntiwong 68 Narissara Eiamkanitchat 438 Narissara Jariyapan 20 Narong Sikhiram 466 Natanee Vorayos 164 Nataya Sutthanont 29 Nateewattana Jintapat 332 Nateewattana Jomjun 332 Nathawit Pongpun 138 Nattanee Vorayos 197 Nattapong Sangsirakoup 451 Nattapong Swangmuang 108 Nattaporn Chaiyat 85, 198 Nattasak Krittigamas 423 Nattawit Pomma 156 Nattawut Kulsatien 146 Nattaya Tawichai 77 Nattee Narkbunnam 43 Natthapong Wongdamnern 71 Natthawan Suwan 483 Natthawud Dussadee 111, 133, 149 517 Nat Vorayos 179, 193, 197 Nednapa Insalud 187 Nethong Namprom 484 Neville R. Watson 109 Ngamjarurojana A. 260, 303, 309, 377 Ngamjarurojana Athipong 221, 244, 261, 279, 379, 390 Nganvongpanit K. 259 Ngaojampa Chanisorn 331 Ngo-Giang-Huong Nicole 265 Nia Kurniati 23 Niamsup Hataichanoke 233, 294 Nicole Ngo-Giang-Huong 27 Nik Khairulddin Nik Yusoff 23 Nimit Morakote 19 Nimmanpipug Piyarat 300, 316, 327, 331, 378, 394, 401 Nimnoi Pongrawee 270, 297 Nipapon Siripon 134 Nipon Theera-Umpon 108, 160, 167, 170, 438, 442 Niramon Utama-ang 435 Niranatlumpong P. 281, 404 Nirun Vanprapar 13 Nirut Naksuk 455 Nisachon Katsamak 95 Nisaratanaporn Ekasit 258 Nitaya Boontim 496 Nithiya Rattanapanone 87, 417 Nochaiya Thanongsak 236, 323, 402, 409 Nokorn Thonglek 129 Nokthai Prontipa 330 Nomura Mika 294 Nongkran Viseskul 484, 489, 490 Nopakoon Koonlertgit 61 Nophadon Luangpirom 97 Nophawan Bunchu 36 Noppadol Kongsricharoen 198 Norbert Gattermann 32 Norfun Poachanee 354 Nottasorn Plipat 13 N. Panyoyai 142 518 N. Siripon N. Tippayawong Nualchareon Mantana Nuanlaor Thawinchai Nuntanee Satiansukpong Nuntasaen Narong Nuthapong Ukarapol Nuttapol Chruewkamlow Nuttapon Sukitsuksawas Nuttawut Dussadee Nuttida Chanhom 107 121, 172 215 471 11 206 45 51 415, 416 194 126 O Ohdaira Yasuo Okorie Anyaele Olaree Nawikkawanit Olga Martincova Ong-Chai S. Opun Supab Orawan Louthrenoo 271, 336 57 448 418 259 358 49 P Paccamon Thammitsakun Pagakrong Lumbiganon Paiboonworachat Sahathaya Pairat Trakarnsirinont Paitoon Narongchai Pakit Bunsut Panchat Wongchaiya Panhuis Marc In Het Panicha Lorwatcharasupaporn Panich Intra 104, 128, Panjarus Wongyai Pannee Visrutaratna Pannipa Laohapensang Panpen Kruathai Pansombat K. Panuwan Chantawannakul Panyanak B. Paradorn Nuthong Paramet Suttiprapa Parichat Choomporn Parinya Chantrasri 419, 150 23 30 506, 507 63 498 93, 94 296 100 141, 174 433 45 430 496 364 89 242 162 187 84 425, 426 Parinya Khamwongpin 461 Paron Dekumyoy 56 Passakorn Inthasan 161 Patcharee Woragidpoonpol 484 Pathom-aree Wasu 387 Patima Silsupadol 8 Pattarawarapan Mookda 350 Pattraporn Chutidumrong 424 Pattra Thanarattanakorn 43, 48, 51 Paul K. Chu 72 Pavena Intaraksa 471 Pawena Limpiteeprakan 163 Pawini Temdi 510 Peanchai Khamwong 5, 6, 9 Pearce John T. H. 210, 351 Peberdy John F. 219 Pedugsorn Mayuree 473 Peerapol Jirapong 173, 459 Peerapong Doungkaew 501 Peerapornpisal Yuwadee 215, 240, 252, 285 Peerasak Lerttrakarnnon 33, 35 Pengchai Petch 332 Pengpat Kamonpan 215, 262, 272, 315, 343, 349 Pennung Warnitchai 452 Penpan Khanrin 69, 82 Pensak Jantrawut 65, 73 Petronella Muresan 13 Pewpan M. Intapan 19, 52 Phanichphant Sukon 224, 241, 269, 271, 289, 336 Phennapha Klangsinsirikul 27 Phiraban Chaithaya 473 Phongdong Pornthana 340 Phongtape Wiwatanadate 16 Phraepakaporn Kunnaja 469 Phruk Aukkarungsri 120 Phrut Sakulchangsatjatai 155, 187, 451, 453 Phuruangrat A. 228, 383 Phuruangrat Anukorn 212, 225, 226, 229, 230, 238, 308, 321, 324, 326, 382, 386, 391, 407 Phutrakul Suree 214 Pichaya Boonprasom 89 Pichaya Boonprasom Poonlarp 420 Pichler Thomas 278 Pierre Fenaux 32 Pietravalle Stephane 396 Pilapong Chalermchai 306, 319, 324, 347, 384 Pimlak Charoenkwan 31, 48 Pimmas Tapanya 49 Pimonrat Sakdasirisathaporn 31 Pimpaporn Klunklin 485 Pimraksa K. 323 Pimraksa Kedsarin 385 Pinruan Umpava 338 P. Intra 172 Pinyou Piyanut 291 Piriya Thongchiew 116 Pisitpipathsin Nuttapon 245 Pisutti Dararutana 74 Pitiwat Wattanachai 459 Pitoon Narongchai 37 Pitsamai Arwakulpanich 464 Piyaboon Nilkaew 491 Piyanan Taweethavonsawat 52 Piyanat Chang-ngern 419 Piyaphong Pantana 191 Piyarat Suntarattiwong 39 Piya Trevittaya 472 Piyawan Sawasdisingha 488 Piyawit Moonkham 416 P. Keawfoonrungsie 166 P. Khunja 166 Pojanakaroon Teraboon 354 Pojprapai S. 260 Polkae Vacharachaisurapol 35 Pompimon Wilart 206 Ponchai Rachtanapun 88 Pongpaibul Yanee 215 Pongpan Sooksarn 118 Pongpol Prompa 134 Pongruk Sribanditmongkol 34 519 Pongsak Holimchayachotikul 441 Pongsaksri Maethisa 473 Pongsathorn Dhumtanom 425 Pongsilp Neelawan 270, 297 Pongsri Keitlertnapha 489 Ponsuksili Siriluck 213 Pookmanee Pusit 224 Pope Kosalaraksa 27 Poramate Sittisun 124 Pornchai Rachtanapun 85, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 100, 101, 102 Pornkitprasarn Witaya 265 Pornpun Subpaiboongid 489 Pornthiva Takwang 433 Porntip Wattanavittawat 472 Posri Leelapat 3, 4 Pothacharoen P. 259 Potong R. 253, 257, 280 Prachaya Siriya 192 Pracha Yeunyongkul 453 Pradchayaporn Thisar 484 Pradit Terdtoon 155, 158, 187, 451 Pradya Somboon 41, 57, 58 Prakaimuk Saraithong 429, 481 Pramoj Ruamsuke 418 Pranee Mahasakpanb 25 Pranom Tansukanun 497 Prapai Dejkhamron 48 Prapamontol Tippawan 350 Prapatsorn Lohwanta 198 Prasanta Saikia 41 Prasatkhetragarn Anurak 244, 263, 403 Prasert Rerkkriangkrai 139 Pravit Limueadphai 174 Preecha Sriprapakhan 450 Preeyaporn Srasuebkul 23 Premanode Bhusana 316 Premchai Moolla 439, 440 Pripdeevech Patcharee 235, 389 Promputtha Itthayakorn 219 P. Sakulchangsatjatai 142, 165 520 P. Terdtoon P. Thanompongchart Puchmark C. Pumsalid Kanchana Puninagool Wattapong Punjasawadwong Y. Punpong Lekhakula Punya Atchara Punyawudho K. Pusadee Kosalaraksa Putipong Bookamana P. Yeunyongkul 142, 165 121 342 206 234 21 89 293 232 39 33 165 Q Quyen Dang Thi Mong 98 R Rachata Lumkul 43 Rachtanapun P. 254 Radchaneekorn Nadsasarn 49 Radom Pongvuthithuma 112 Rafael Auras 93, 102 Rakariyatham Nuansri 310, 356 Raksujarit A. 349 Ralph E. Harbach 58 Ram Yogev 13 Randeep Singh 155 Ratanakosol Janyaporn 265 Ratanawimarnwong Nuanlaor 203 Ratchadawan Ngoen-klan 34 Ratiporn Haruenkit 418 Rattapol Pornprasit 426 Rattika Sae-Tung 31 Rattikorn Yimnirun 71, 75, 81 Raweeroj Jintawiwat 443 Rawee Taweephol 31 Rawiwan Hansudewechakul 23, 27, 39 Rayanakorn Mongkon 366 Raymond A. DeCarlo 130 Rebecca S. Gelman 27 Rerkasem Benjavan 247 Revathy Nallusamy 23 Rianyoi R. 253, 257, 280 Risa Okonogi 26 Rodanant O. 21 Rojas Carlos 339 Rolf G. Werner 68, 69, 82 Rotsukon Jawana 120 Ruanpanun Pornthip 209 Ruengsitagoon Wirat 292 Rujijanagul G. 262, 272, 315, 342, 343, 349 Rujijanakul Gobwute 245 Rujirawat S. 260 Rujiwatra Apinpus 307, 406 Rungchat Chompu-inwai 182, 439, 440, 454, 493 Rungjindamai Nattawut 338 Rungmanee Jomthong 173 Ruth Ruggles 17 Ruyan Guo 81 Ryumond Honda 24, 25 S Sabsanong Prapan 265 Saengnil Kobkiat 251, 392 Saengusa Khemaleelakul 481 Saenphet Kanokporn 277 Saenphet Supap 277 Saipanya Surin 357, 405 Saisunee Liawruangrath 64, 79 Sakai Tadao 204 Sakda Pruenglampoo 3, 4 Sakgasit Ramingwong 460 Sakornrat Khongkhunthian 429 Sakulrat Kunyatep 493 Samart Pakakasama 43, 53 Sangchan Walaya 366 Sangprasert Waleepan 300, 378, 401 Sangtiwa Suriyong 423 Sa-Nguangsak Thanapornpoonpong 423 Sanguansermsri Torpong 360 Sangwijit K. 345 Sanmee Rarunee 313 Sanong Amaroek 170 Sanparwat Vithayasai 197 Sansanee Auephanwiriyakul 160, 167, 170, 438, 442 Santhaveesuk Theerapong 273, 275 Santi Chanwikrai 168 Saouy Maliwan 332 Saowarunee Sangsrijan 62 Sarakonsri T. 325 Sarakonsri Thapanee 357, 381, 397 Sarapee Chunthawom 447 Sarapirom S. 345 Sarawut Pongleerat 495 Sardsud Uraporn 252, 320 Sarinya Sriphetcharawut 10 Sasat Daranee 473 Sasitorn Wongruong 91 Sasiwimol Ubolyam 27 Sate Sampattagul 115, 164 Satiansukpong Nantanee 473 Satienperakul Sakchai 243, 340 Satit Phiyanalinmat 424 Sauwalak Opastirakul 15 Sawaddiruk Passakorn 30 Saweat Intarasiri 72 Sawitree Chiampanichayakul 51 Sayam Kaewvichit 62 Saysin Suwanna 304 Schmidt-Vogt Dietrich 282 Seeharaj Athittaya 406 Seielstad Mark 298 Sein Min 57 Sein Thaung 57 Sermsak Uatrongjit 103, 109, 152 Sethapong Sethabouppha 492 Setthaya Naruemon 385 Shank Lalida 330 Shela Gorinstein 418 Sherman S.G. 50 Shinbo Kazunari 269, 271, 336 Shinoda Yasuhiro 381 Shiojiri M. 305 Shiozawa Hidetsugu 278 Siangcham Tanapan 277 521 Siengdee P. 259 Siew Moy Fong 23 Silalom Somyot 218, 276 Sinchaikul Supachok 214 Sineenad Somboonanek 415 Singjai P. 404 Singjai Pisith 278, 296 Singkamanee C. 251 Singthong Urailuk 207 Singtripop Tippawan 286, 398 Siraseth Mongkolupatham 143 Siriluk Sangsrijan 62 Sirinontakan Surat 362 Sirinun Boripuntakul 7 Siriprapa P. 301 Siripun Narongchai 63 Siriwan Kammuen 84 Siriwan Nissaikla 145 Siriwan Ong-chai 478 Siriwat Wattanapanich 480 Siriwitayakorn Kingkeo 246 Sittipiyasakul Veerachai 265 Sittiporn Suwannamit 20 Sittiruk Roytrakul 20 Siva Achariyaviriya 132, 138, 162, 447, 457 Siwadol Supapia 180 S. Kantawanichkul 136, 189 S. Nirunsin 183 S. Noimanee 166 S. Nuchprayoon 105 Soisungwan Sataruge 25 Sokhuma K. 318, 353 Somboon Sangmaneedet 52 Somchai Lapanantnoppakhun 83 Somchai Pattana 444 Somchit Kiatwattanacharoen 484 Somjai Kanjanapongkul 43 Somjai Karnchanawong 117, 145, 163, 198 Somkiat Jaturonglumlert 154 Sompon Wongtom 140 Somporn Chotinaruemol 48 522 Somporn Sungkarat 7, 8 Somporn Wangruangsathit 43 Somrak Rangkakulnuwat 26, 53 Somruthai Tunma 96, 102 Somsak Wongwaiwate 37 Somsiri Sattayapanich 145 Songkran Panthurat 493 Songmee Naratip 278, 296 Songyot Anuchapreeda 51 Soontorn Suwanhem 449 Sophon Sinsang 451 Sorawat Thongsahuan 20 Sreesattabud Tharathip 370 Srichomthong Kitlada 337 Srikitikulchai Prasert 337 Srikummool Metawee 298 Srilomsak S. 260 Sripaoraya Worathip 360 Srirojn B. 50 Srisombat L. 267 Srisombat La-Ongnuan 279, 379 Srisuda Sae-tang 3, 4 Srivibool Rattanaporn 387 Sriwichai Saengrawee 269, 271, 336 Stephan K Weiland 17 Stephen J. Kerr 27 Stephenson Steven L. 339 Streck T. 364 Suantai S. 373, 375 Suantai Suthep 201, 204, 205, 207, 211, 239, 287, 371, 372, 410, 411 Suchada Nokthuan 437 Suchada Vearasilp 418, 423 Suchat Hongsiriwon 13, 39 Suchaya Silvilairat 38 Sudarat Auttarat 495, 497 Sudchit Chungpivat 52 Sudjai Suanta 31 Sue Turale 489 Suebsak Sanyakiatikun 415 Sugree Gasorntgatsara 503 Sukchareon I. 59 Sukchotiratana Morakot 387 Sukhumanant Thammanoon 265 Sukrapee Insee-ard 49 Sukum Eitssayeam 67, 77, 101 Sumitra Poovarodom 418 Sumpun Chaitep 110, 119, 123, 147, 185 Sumpun Chaithep 133 Sung-U Sasitorn 473 Su-on Chainansamit 43 Supalak Palee 164 Supanee Chounchay 8 Supanida Winitchai 70 Supansa Dudngon 432 Supanuch Saipang 435 Supaporn Chinchai 10, 11 Supaporn Nakbunlung 415, 416 Supara Grudpan 83 Suparat Kanjanavanit 27, 44 Supassara Sirabanchongkran 480 Supatra Sirichotiyakul 31 Supawadee Putthinoi 470, 474 Suphan Sutthawan 285 Suphavilai Chaisuree 350 Supjatura Volaluck 311 Suppakarn Chansareewittaya 458 Surachai Singhtoraj 491 Surakrit Nattaradol 441 Suranan Noimanee 113, 114, 445, 446 Suraphol Phuvichit 180 Suraphon Chumklin 167 Surapon Wiangnon 43 Surasing Visrutaratna 3, 4 Suratsunya T. 59 Sura Tundee 155 Sureeporn Suwanna-osod 484 Surin Jiraniramai 33 Surin, Saipanya 357 Sutcliffe C.G. 50 Sutee Yoksan 12 Suthat Surawang 435, 437 Sutthana S. 216 Suttiarporn Panawan 389 Suttichai Krisanaprakornkit 476, 477 Suttichai Premrudeepreechacharn 109 Suwannarach Nakarin 335 Suzuki Toshisada 233 T Taesotikul Tawat 213 Tajima Shigeyuki 294 Takahashi W. 288, 374, 376 Tamaekong Nittaya 289 Tanakorn Apornpong 27 Tangchitsomkid Nuchanart 209 Tang Mingjie 185 Tangonan Andrew A. 241 Tangsritrakul J. 267, 309 Tanin Bhoopat 26, 54 Tanongkiat Kiatsiriroat 111, 116, 129, 140, 148, 149, 154, 164, 175, 177, 184, 190, 193, 194, 196, 197 Tanudsintum S. 21 Tanyathip Jaimulwong 27 Tarinee Chaiwong 36 Tashiro K. 378 Tavitiya Sudjaritruk 38 Tawan Mengthaisong 27 Tawat Samana 177 Tawee Chotpitayasunondh 13 Tawee Tunkasiri 67, 77 Tawichai N. 315, 343 Taworn Benjanarasuth 455 Teerarat Sattayarak 95 Teerasong Saowapak 203 Teerawutgulrag Aphiwat 310 Teshima Norio 203 Thaisuchat Haruthai 206 Thanachayanont Chanchana 210, 381 Thanakron Paboug 125 Thanaporn Ngarmprapasom 119 Thanasit Wongsiriamnuay153, 171, 195 Thanasuthipitak Panjawan 302 Thanasuthipitak Theerapongs 302 Thanes Chaichara 133 Thanyaporn Boonphokaew 422 523 Thanyawat Rattanasak 508 Thanyawee Puthanakit 12, 23, 27, 39, 44, 47, 49 Thapakron Kruaraya 415 Thapanee Kruaraya 415 Thapanya Decha 276 Tharavichitkul Ekkasit 352 Thaung Hlaing 57 Thavornyutikarn P. 364 Thavornyutikarn Prasak 332 Thawan Sucharitakul 127 Thianchai Aksrondit 415 Thiemsorn Worapong 200 Thienthong S. 59 Thira Sirisanthana 12, 44, 47 Thitilertdecha Nont 310 Thompson S. P. 368 Thomson N. 50 Thongchai Fongsamootr 143, 144, 191 Thongchai Wisanu 202, 361 Thongkuntha Sutheera 320 Thongsanitgarn Pailyn 268 Thongsrisomboon Pawinee 243 Thongtem Somchai 212, 220, 223, 225, 226, 227, 228, 229, 230, 238, 256, 278, 283, 306, 308, 319, 321, 324, 326, 347, 382, 383, 384, 386, 391, 407, 413 Thongtem Titipun 212, 220, 223, 225, 226, 227, 228, 229, 230, 238, 256, 306, 319, 321, 324, 326, 347, 383, 384, 386, 391, 407, 413 Thraravichitkul Prasit 311 Thungprasert S. 325 Thwaites Richard 396 Tim R. Cressey 13 Tinakon Wongpakaran 33 Tinnapat Mongkontep 145 Tipcompor Narongrit 223, 230 Titima Sukmark 85 Tokuyama Shinji 387 524 Tomomi Kameoka 488 Tongjit Thanchomnang 52 Torkittikul Pincha 236, 408 Torpong Sanguansermsri 31 Torsak Bunupuradah 27 To Setha 57 Toshihiro Akihisa 65, 73, 76 Toshiko Nakayama 488 Tossaporn Wimuktacom 186 Totrakool S. 364 Towta Sripen 246 Traichaiyaporn Siripen 367 Traisaithit Patrinee 265 Traisathit Patrinee 352, 362 Triamnak N. 377 Trichaiyaporn Siripen 332 Tuchinda L. 59 Tulathip Suwanlerk 39 Tungpradit Rudeewan 214 Tunkasiri T. 262, 315, 343, 349 Tunkasiri Tawee 245, 312 Tunma S. 300 Tunwadee Klong-klaew 34 U Ubon Pirunsan 5, 6, 9 Ubonrat Siripatrawan 431, 434, 436 Udom Chaithong 29 Umaporn Siangphoe 39 Ungsethaphand Thepparath 252 Unruan M. 267, 377 Unruan Muangjai 221, 244, 261, 370, 403 Uraporn Sardsud 419, 422, 426 Usanee Thongchai 415 Usanee Vinitketkumnuen 128 Usawadee Chanasut 417 Uthaibutra Jamnong 251, 392 V Vaidhayakarn Chawapich Valery Zeitoun Van Dyke Russell B. 249, 282 416 265 Van Zee J. W. 232 Vannarat Sornthep 327 Vas Dev 41 Venediktos Kapetanakis 17 Viboon Changrue 147 Vichai Laosombat 43 Vicha Sardsud 426 Vilaithong T. 325 Vilaithong Thiraphat 327 Viraphong Lulitanond 52 Virat Sirisanthana 12, 13, 23, 38, 39, 44, 47, 49 Visut Baimai 20 Vittayakorn Soisuda 473 Vittayakorn W. C. 284, 317, 341 Vivek Agarwal 130 Vonthanak Saphonn 23, 27 Vorapoj Pothacharoen 111, 194 W Wanchai Maleewong 19, 52 Wandee Taesotikul 62 Wang John 312 Wangkarn Sunanta 366 Wangpakapattanawong Prasit 282 Wanichacheva Nantanit 199, 380 Wannakon Anucha 200 Wannapira Wiroj 265 Wannapop Surangkana 225 Wannipa Bunrayong 470 Warangkana Lohcharoenkal 82 Waravut Printrakoon 455 Warintorn Ruksiriwanich 66 Warrit Natapot 314 Warunee Kumsaiyai 471 Wasana Prasitsuebsai 13 Wasan Pinatae 149 Wasin Wongwilai 83 Wasu Pathom-aree 419 Watana Sukeepaisarnjaroen 19 Watchara Kasinrerk 51 Watcharapasorn A. 208, 281, 301, 404 Watcharapasorn Anucha 255, 268, 370 Watcharee Tantiprabha 31, 48 Watpathomsub Supranee 199, 380 Wattana Chartapisak 15 Wattanapong Suttapak 160 W. Duangjaisak 136 Weerachai Arjharn 179 Wej Choochote 20, 29, 46, 52, 57 Werawan Ruangyuttikarnd 24, 25 Whangchai K. 251 Whangchai Niwoot 252 Wicharn Luesomboon 27 Wichuda Jiraporncharoen 61 Wilaiwan Leenakul 135 Wilart Pompimon 477 Wilawan Kumpoun 78, 80 William T. Shearer 27 Willoughby Tun-Lin 57 Wimalin Laosiritaworn 75 Wimmers Klaus 213 Wimonrat Teeyakasemd 25 Winiyakul Narong 265 Winthana Kusirisin 35, 65 Wipasa Jiraprapa 350 Wipawadee Wongsuwan 192 Wirat Nakkrongdee 129 Wirojanupatump S. 404 Wirojupatump S. 281 Wirongrong Tongdeesoontorn 91, 92 Wisanu Thongchai 64, 79 Wisitsoraat Anurat 289 Wisut Pattanaporn 429 Witaya Swaddiwudhipong 24, 25 Witchapong Issarangporn 76 Wittanalai Suttida 356 Wittaya Daungthima 497 Wittaya Petdachai 39 Wolfgang Lücke 423 Wongchaiya P. 254 Wongdamnern N. 303, 309, 377 Wongkeo W. 323 Wongkeo Watcharapong 236, 237, 409 Wongkham Weerah 214 Wongkot Wongsapai 188, 463 525 Wongmaneerung R. 264 Wongpia Aphinya 222, 311 Wongpornchai Sugunya 235, 389 Wongputtisin Pairote 320 Wongratanaphisan Duangmanee 273, 275 Wongrat Ekasiddh 358 Wongsaenmai Supattra 221 Wongsawad Chalobol 329, 348 Wongsawad Pheravut 329 Wongsawan Kanruethai 311 Wongwilai Wasin 412 Woralun Boonyasurat 415 Worapaka Manosroi 66, 68, 69, 70, 73, 76, 82 X Xu Shuhua 298 Y Yana Janchai 327 Yao J. C. 288 Yasintinee Aimyuak 188 Yasushi Otsuka 20 Yatakoat Pornsak 205 YiHong Li 429 Yimnirun R. 253, 260, 263, 264, 267, 280, 284, 303, 309, 317, 377 526 Yimnirun Rattikorn221, 244, 250, 261, 279, 293, 334, 370, 379, 390, 403 Yin Q. R. 317 Y. Khunatorn 183 Yoiprommarat Seangaroon 337 Yong Poovorawan 20 Yongyuth Chalearmchart 293, 420 Yongyut Laosiritaworn 71, 75, 81 Yoshio Tsuda 58 Yotnoi Bunlawee 406 Youpensuk Somchit 247 Yuenyong Panjasawatwong 180 Yu L. D. 345 Yu Liangdeng 72, 331 Yuriko Nomoto 488 Yuthana Phimolsiripol 432, 433, 434, 435, 436, 437, 438 Yuthapong Somchit 176 Yuttana Kumsuwan 462 Yuttana Tongtuam 492 Yvonne Linton 57 Yvonne-Marie Linton 41 Z Zeng H. R. 317