Institute for Agricultural and Fisheries Research - ILVO
Transcription
Institute for Agricultural and Fisheries Research - ILVO
Institute for Agricultural and Fisheries Research Activity report 2006 Flemish government Institute for Agricultural and Fisheries Research Activity report 2006 Flemish government www.ilvo.vlaanderen.be Flemish government Responsible publisher Prof. dr. ir. Erik Van Bockstaele Coordination Photographs Lay-out Translation How to order dr. ir. Johan Van Waes, Nancy De Vooght, Karin Van Peteghem ILVO Nancy De Vooght Frank Lunn by e-mail [email protected] by telephone +32 (0) 9 272 25 00 by mail Burg. Van Gansberghelaan 96, B-9820 Merelbeke mention “Activity Report 2006” 9 1. Management and Units 12 1.1 Animal Sciences 14 1.1.1 1.1.2 1.1.3 Functional Animal Nutrition Animal Husbandry and Welfare Fisheries 15 16 19 1.2 Social Sciences 20 1.3 Plant Sciences 22 1.3.1 1.3.2 1.3.3 Applied Genetics and Breeding Growth and Development Crop Husbandry and Environment 23 27 29 1.4 Technology & Food 30 1.4.1 1.4.2 1.4.3 1.4.4 Product Quality and Innovation Food Safety Business Unit and Service Centre Agricultural Engineering 32 33 34 34 2. Important research results 37 2.1 Animal Sciences 37 2.1.1 2.1.2 2.1.3 2.1.4 2.1.5 2.1.6 2.1.7 2.1.8 Milk urea concentration as an indicator of the N excretion of dairy cows into the environment 37 Reducing ammonia emissions from pig houses through feeding strategy 38 Research into alternatives for antimicrobial feed additives 39 Qualitative feed restriction in beef cows 40 Early and reliable detection of boar taint 41 Integration of animal welfare in the food quality chain 42 Value creation in Belgian sea fisheries 44 Biological fisheries research: there is more to it than just counting fish 46 2.2 Social Sciences 48 2.2.1 2.2.2 Modelling the agricultural sector Corporate social responsibility (CSR) 48 50 Table of contents Introduction Table of contents 2.3 Plant Sciences 2.3.1 2.3.2 2.3.3 2.3.4 2.3.5 2.3.8 New insights into the origin of the cultivated apple (Malus x domestica) 52 Valorisation of the genetic diversity of wild roses 53 Breeding for resistance against nematodes in green manure crops (catch crops)54 Inventorising predatory mites in tree nurseries 55 Infectivity under field conditions of Clavibacter michiganensis subsp. sepdonicus, causal agent of bacterial ring rot of potato 57 Population dynamics of the rootknot nematode Meloidogyne chitwoodi under different field crops and black fallow 58 Fifteen years of cooperation with the former Eastern Block countries: The democratisation process and the acquis communautaire 61 SANCO EC - trial for grasses 63 2.4 Technology & Food 2.4.1 2.4.2 2.4.3 Residual enzyme activity in UHT-milk and milk powders 64 Effect of a changed milk fat composition on the quality and processability of milk 65 Mechanisation: A good application technique for more efficient entomopathogenic nematodes 67 Livestock engineering and constructions - Automatic detection of lameness in dairy cattle 69 Protection and safety engineering - Ammonia emissions from naturally ventilated buildings 71 PCBs in eggs originating from different production systems 73 Residue formation of sulphonamides in honey by migration from contaminated beeswax 74 Bacteriological contamination and infection of shell eggs in the production chain 75 Identification and harmful effects of the aerobic spore microbiota in raw milk 76 Reduction of Salmonella shedding by mid-chain fatty acids and botanic compounds in a in vitro simulation of the pig’s cecum 77 2.3.6 2.3.7 2.4.4 2.4.5 2.4.6 2.4.7 2.4.8 2.4.9 2.4.10 3. Extra highlighted 3.1 Comparison of hygiene status and animal welfare in furnished cages and noncage systems for laying hens Dietary ω-3 fatty acids: effect on performances and incorporation in milk and meat using the rabbit as model 2006: A productive publication year for ILVO-fisheries Social Sciences Unit: new paradigms Centre for Plant Diagnosis: in the spot Measuring residual nitrates in soils: elevated nitrogen utilisation as solution? Cooperation of ILVO-T&V with Flanders’ FOOD projects 3.2 3.3 3.4 3.5 3.6 3.7 52 64 78 78 79 80 82 84 85 87 3.9 4. Role of ILVO-T&V in the Belgian National Reference Laboratory (NRL) Consortium for GMOs 88 PreventAgri Vorming/Formation encourages safety in agriculture and horticulture 89 Public Service 91 4.1 Reference laboratory for research on animal nutrition and the nutritional value of animal products 91 4.2 Social Sciences 92 4.3 Centre for Diagnosis of Plant Pests 92 4.4 Varieties developed by ILVO-Plant - Cultivars on variety lists and commercialised 94 4.5 TAD FarmCOMPOST 96 4.6 Variety Testing – Post-control trials – Seed Testing Laboratory 98 4.7 Addition of accredited laboratory analyses in the framework of food authenticity and food safety 100 4.8 New hygiene directives in the pilot plant 102 4.9 TAD “Hoevezuivel” - Farm dairying in practice 102 4.10 Agromech 103 4.11 AgriCONSTRUCT 104 4.12 Laboratory for Spray Application Techniques 104 4.13 Quality control in connection with the maintenance of milk instalations 105 5. Publications 106 5.1 Articles published in journals and included in the Science Citation Index 106 5.2 Other scientific articles 107 5.3 Books and chapters in books 108 5.4 Proceedings and abstracts of symposia and congresses 108 5.5 Vulgarised articles 112 5.6 Ph. D. theses 114 5.7 ILVO communications 114 5.8 Policy-relevant notes and reports in the framework of mandated and other tasks 114 6. Theses and training reports 117 7. Communication 118 7.1 7.2 Press contacts and visits of Belgian and foreign delegations 118 Activities, workshops and courses organised by or in cooperation with ILVO 119 Table of contents 3.8 Introduction Dear reader, This is the first activity report of ILVO, the Institute for Agricultural and Fisheries Research. Since 1 April 2006, both the former Agricultural Research Centre (CLO) and Centre for Agricultural Economics (CLE), together with their corporate identities were merged into the newly established ILVO. From 1 January 2007 the funds for developing sustainable agricultural systems were transferred to ILVO (unit Social Sciences). ILVO is an internal independent agency with a corporate identity and comprises four units, each with several research areas. The management agreement with the Minister competent for ILVO provides the contours in which ILVO, as a scientific institute, will carry out its mission of basic and policy-supporting research and services. Performance indicators quantify the results obtained. The structure of ILVO has been elaborated in the past year and efforts were made to organise and develop human resource management, research policy, internal and external communication, cooperation and supporting services. This process is not completed yet, but I am grateful to all staff for their positive attitude and cooperation towards the transformation process that occurred during 2006. The results of a personnel survey showed great satisfaction concerning the labour conditions and value-perceptions. Based on these results we will try, where possible, to set up suitable transformation routes. frastructure and support from the Agency for Facility Management should eventually solve various infrastructure problems. In the past year ILVO was an open house. There were internal team-building sessions per unit, thematic meetings with colleagues from other entities within the Ministry of Agriculture and Fisheries, visits and consultations with Minister Fientje Moerman, ‘Boerenbond’, experimental research and extension centres, cabinet members and students. ILVO was also often referred to in the press. For 2007, we foresee ILVO cooperation in Agriflanders and the Flanders’ Day on 16 April. In June we will celebrate 75 years of agricultural research in Flanders with the anniversary of the founding of the former Plant Breeding Research Station, the activities of which are now integrated in ILVO-Plant Sciences. Finally, I take this opportunity to express my gratitude to all staff and all those who were directly or indirectly supportive in realising the functioning of ILVO and who have put their trust in ILVO. I am also much indebted to all audit, supervisory management and advisory bodies for their essential and much appreciated support for ILVO. Enjoy reading this activity report, ILVO believes in: - a positive future for Flemish agriculture and fisheries - objective, integral and independent research within a scientific organisation - internal and external cooperation. ILVO’s core business is to be found in the area of: - the execution of qualitative prominent research and public service for the policy-makers and the sector - the valorisation of research results - the dissemination of research results through open communication. High quality research relies on both human resources and facilities. Both the expansion of the scientific staff and the ILVO-PhD-fund will strengthen the critical mass. The step-by-step implementation of the Master plan for the inErik Van Bockstaele, Administrator-General of ILVO. Administrator-general (N) Prof. dr. ir. Erik Van Bockstaele COWEDI (N + N-1 + GD + sci. dir.) internal advice Consultation Organ Government/Policy - ILVO Research Director (Prof. dr. ir. Maurice Moens) Research support services - project control, scientific equipment, sci. output - IT and statistics (dr. ir. Koen Grijspeerdt) Communication Director (dr. ir. Johan Van Waes) Communication cel Advisory organ: BOC, Ethical Commission Social Sciences head of the unit (x) Research Area -Functional Animal Nutrion dr. ir. Ludwig Lauwers dr. ir. Gerard Huyghebaert ir. Dirk Van Lierde -Animal Husbandry and Welfare Prof. dr. ir. Daniël De Brabander -Fisheries dr. Kris Cooreman Head of a research area: scientific director (x) function still open 10 Board CP (Corporate Personality) General Director ir. Joris Relaes (detached) Management Committee Animal Sciences head of the unit (x) Advisory Committee with working groups for each knowledge unit - External advice MSS (Management and Supporting Services) - secretariat - management of Staff and Control Financial Services - financial and operational audit (x) - finances en budget, accounting (Funding + CP) Support - environment, safety, welfare (ir. Frank Lagaisse) - IT, facility services, logistics, general infrastructure Plant Sciences head of the unit (x) Research Area -Growth and Development (x) -Applied Genetics and Breeding dr. ir. Johan Van Huylenbroeck -Crop Husbandry and Environment Prof. dr. ir. Lucien Carlier -Crop Protection dr. Martine Maes -Business Unit and Service Centre (x) Technology & Food head of the unit (x) Research Area -Agricultural Engineering Prof. dr. ir. Bart Sonck -Food Safety dr. Marc Heyndrickx -Product Quality and Innovation dr. Marc De Loose -Business Unit and Service Centre dr. Lieve Herman Corporate Personality Management Commission Members ILVO: - Prof. dr. ir. Erik Van Bockstaele, administrator-general, Chairman - dr. ir. Kristiaan Van Laecke, scientific attaché - Prof. dr. ir. Daniël De Brabander, scientific director - dr. Lieve Herman, scientific director - ir. Dirk Van Lierde, scientific director Leading official of the department Agriculture and Fisheries: - Mr. Jules Van Liefferinge, secretary-general Representative of the Flemish Minister competent for science and technology: - dr. Monika Sormann (until 31/08/06) - dr. Kathleen D’Hondt (since 01/09/06) Representative of the Flemish Council for Agriculture and Horticulture: - Mr. Jos Matthys (until 31/12/06) - Mr. Philippe Appeltans (since 01/01/07) Representative of the Inspection of Finances: - Mr. Kurt De Bruyne, inspector-general Expert policy domaine Agriculture and Fisheries: - ir. Hector Willocx, projectleader ALV 11 1. Management and units Management ILVO - Management • • • • • • • • • Prof. dr. ir. Erik Van Bockstaele, administrator-general Prof. dr. ir. Maurice Moens, research director (since 1/04/06) dr. ir. Johan Van Waes, communication director (since 1/04/06) ir. Frank Lagaisse, prevention, safety, environment and welfare dr. ir. Koen Grijspeerdt, IT (since 1/04/06) ing. Steven Cools, IT ing. Sabine Nelis, IT ing. Ellen Claeys, IT André Roelandts, coordination Mission ILVO ILVOs mission consists of the execution and coordination of policy-supporting scientific research and the accompanying public service with a view to sustainable agriculture and fisheries in an economic, ecological and social perspective. Based on scientific disciplines ILVO will build up the necessary knowledge for improving products and production methods, for monitoring the quality and safety of the end products and for improving the policy instruments as a basis for the development of the sector and rural policy. ILVO will inform the authorities, the various sectors and society at regular intervals. 12 Some figures about the staff of ILVO on 31/12/06 Flemish government Male/FTU Female/FTU Total/FTU Average age A-level 55/53.0 27/19.9 82/72.9 44 B-level 16/16.0 20/17.7 36/33.7 36 C/D-level 68/64.9 70/49.6 138/114.5 47 139/133.9 117/87.2 256/221.7 45 Total Corporate personality ILVO Male/FTU Female/FTU Total/FTU Average age A-level 43/42.0 52/49.8 95/91.8 31 B-level 23/21.9 32/26.9 55/48.8 30 C/D-level 55/53.1 28/21.7 83/74.8 38 Total 121/117 112/98.4 233/215.4 34 General total Male/FTU Female/FTU Total/FTU Average age A-level 98.95.0 79/69.7 177/164.7 37 B-level 39/37.9 52/44.6 91/82.5 33 C/D-level 123/118.0 98/71.3 221/189.3 44 Total 260/250.9 229/185.6 489/436.5 39 13 Functional Animal Nutrition Animal Husbandry and Welfare Animal Sciences 1.1 14 Scheldeweg 68 B-9090 Melle Tel.: +32 (0) 9 272 26 00 Fax: +32 (0) 9 272 26 01 [email protected] Functional Animal Nutrition Animal Husbandry and Welfare Scientific director Scientific director dr. ir. Gerard Huyghebaert Prof. dr. ir. Daniël De Brabander Researchers Researchers lic. Johan Aerts dr. ir. Johan De Boever dr. ir. Evelyne Delezie (since 1/10/06) dr. ir. Sam Decampeneere dr. ir. Marijke Lippens (until 30/06/06) dr. Sam Millet ing. José Vanacker dr. ir. Nathalie Warnants (until 30/06/06) ir. Marijke Aluwé (since 01/09/06) ir. Stephanie Buijs (since 06/10/06) ing. Marc De Paepe dr. ir. Leo Fiems dr. Nicoline Geverink ir. Luc Maertens lic. Griet Nijs dr. Frank Tuyttens lic. Els Van Poucke dr. ir. Monique Van Oeckel 1.1.1 Functional Animal Nutrition In the domain of Functional Animal Nutrition applied scientific research on animal production is carried out aimed at matching the supply of nutrients with the requirements of cattle, pigs and poultry, taking into account on the one hand a minimum output of minerals into the environment and on the other hand the production of milk, meat and eggs with a high nutritional and health value. Research into nutrition physiology through digestion and balance trials results in a more correct feed evaluation and contributes to an improved nutrient provision for the different animal species and hence to a better nutrient efficacy and a lower environmental pollution. The development of invitro techniques as fast evaluation tools may reduce the number of experimental animals. In this respect, our reference laboratory plays an important role not only by implementing new analytical techniques but also by the ISO 17025 guarantee for quality analyses on both animal feeds and animal products, which is very important for an overall quality control in the food chain. sion into the environment. At the same time, a residue evaluation will be done for anticoccidials and therapeutic medicines in eggs and broiler meat. Besides, there is a need for new dietary strategies to improve N- and P-utilisation and to reduce N- and P-excretion by poultry (laying hens, broiler chickens, turkeys) through research on the effects of multiphase feeding, precision feeding, adapted amino acid profiles, an adapted Ca/P-balance, vitamin D3 and analogues, exogenous phytase, etc. Another objective of functional animal nutrition is to improve, through dietary manipulations, not only the animal physiology and health but also the general and specific quality (with an additional health value for the consumer) and the image of the final product (meat, milk and eggs). In this way the enrichment is possible with n-3 and n-6 poly-unsaturated fatty acids, anti-oxidants and trace minerals. Research topics with dairy cattle are: the use of grains as concentrate replacement, the incorporation of leguminosae in the ration and the use of by-products originating from the production of biofuel. Besides, a project is started to investigate if and how the rumen degradable protein balance (DPB) of dairy cattle rations can be lowered by, for example, improving the synchronisation of energy and protein in the rumen and/or by using rumen protected protein sources. Lowering the DPB reduces the N excretion into the environment. Moreover, protecting soya protein against rumen degradation and the use of rapeseed meals may strongly reduce the import of soybean meal. A project is running with meat pigs to reduce the ammonia emission from pig houses by means of feeding measures. The intention is to evaluate the impact of different feeding strategies and additives on the true ammonia emission, measured under Flemish conditions. Another research project is looking at the impact of dietary protein levels below the optima for maximal performance on nitrogen excretion and meat percentage of meat pigs, in order to find an optimum that takes into account economy as well as ecology. Because of the recent ban on feed antibiotics, there is a continuous need for an objective evaluation of a range of alternatives, e.g. probiotics, prebiotics, enzymes, acids, yeasts, etheric oils and even anticoccidials. The physiology at intestinal level is thereby very important for both intestinal health and nutrient utilisation with a direct impact on the zootechnical performance and the nutrient emis- 15 1.1.2 Animal Husbandry and Welfare This research domain focuses on key aspects of cattle, pig and poultry husbandry in general as well as on animal welfare in particular. Although the research topics are often related to animal nutrition, they concern the wider perspective of livestock production. 1.1 Functional Animal Nutrition Animal Husbandry and Welfare Animal Sciences An important focus of the research on cattle is on the development of feeding strategies for suckling cows. According to the cross-compliance of the European Common Agricultural Policy, the total area of permanent grassland in Flanders should be maintained. On the other hand, the cattle herd is decreasing. Consequently, more attention has to be paid to a maximum and efficient valorisation of grassland. Following the period with restricted feed intake in winter, suckling cows are able to replenish their body reserves during the pasture period. 16 Concerning pig husbandry, group housing of sows and castration of piglets are important research topics. Legislation stipulates that by 2013 pregnant sows ought to be housed in groups. This implies an adapted feeding strategy. Restricted feed allowance, which is common practice for individually housed sows, can be applied in group housing conditions but requires a high investment. Our research focuses on the ad libitum provision of fibrous feedstuffs, ensiled roughages and wet by-products as an alternative strategy with likely animal welfare benefits. In addition, we investigate on 40 sow units the effects of the environment, management and animal parameters on production data, animal health, animal welfare, dust concentration, sanitary status and ammonia concentration in the sow house to help the pig farmer choosing a group housing system suited to the farm conditions and management. Another hot issue in pig husbandry is the surgical castration of male piglets which is a routine practice to prevent the occurrence of boar taint. Research to reduce boar taint without castration and to reliably detect boar taint in living pigs as well as in pig carcasses is on-going. The extent to which boar taint can be reduced by dietary manipulations, choice of breeds, reduced slaughter weight and hygienic measures is being investigated. Furthermore, we aim to identify behavioural or physiological risk-factors in young pigs that predict the likelihood of developing boar taint when they reach slaughter age. Regarding poultry production, our research focuses on the evaluation of different housing systems for laying hens (furnished cages and non-cage systems) and on reducing the negative consequences of metabolic stress in broiler chickens. The continuous breeding efforts to increase growth have delivered broiler strains with exceptional growth performances (2.0 kg at 5 weeks of age). However, such a selection has also led to some negative responses. Using both a modulation of the growth curve as well as growing strains with a slower growth rate, we aim to improve meat quality characteristics and to contribute to a more sustainable broiler chicken production. Across the main livestock species groups, animal welfare research focuses on the development of objective, valid and innovative indicators of animal welfare. We provided evidence, for example, that the degree of (fluctuating) asymmetry is a promising indicator of how well an animal has been able to cope with stress during its development. These separate welfare indicators are subsequently aggregated into a protocol for evaluating the general state of welfare. Within the EU-project ‘Welfare Quality’, the Animal Science Unit contributes to the development of an EU-standard for evaluating the welfare of farm animals. These protocols are then used for investigating issues that are highly relevant to our agricultural policy, such as the stocking density in livestock production. The stocking density of livestock is of paramount importance for profitability and animal welfare. In an on-going research project we aim to find a compromise between profitability and animal welfare concerns. Therefore, the relationships between stocking density-profitability and between stocking density-animal welfare are being determined for two relevant model animals, broiler chickens and rabbits. The importance Flemish citizens allocate to this topic is being explored too. Calf-hutches 17 Fisheries Animal Sciences 1.1 18 Ankerstraat 1 B-8400 Oostende Tel.: +32 (0) 59 34 22 50 Fax: +32 (0) 59 33 06 29 [email protected] Fisheries Scientific director dr. Kris Cooreman Researchers ir. Karen Bekaert ing. Fernand Delanghe lic. Daan Delbare Ir. Wim Demaré ir. Jochen Depestele ing. Sabrine Derveaux lic. Fanny Dooms lic. Hans Hillewaert MSc. Stefan Hoffman dr. Kristian Hostens lic. Bart Maertens MSc. Ine Moulaert dr. Koen Parmentier dr. ir. Hans Polet MSc. Wang Qian (since 16/10/06) dr. Frank Redant lic. Hendrik Stouten ir. Kris Van Craeynest (since 1/10/06) MSc. Els Vanderperren ing. Johny Vanhee ing. Willy Vanhee lic. Jan Wittoeck 1.1.3 Fisheries The core tasks of the Research Area Fisheries (D-VI) are situated in the field of fisheries biology, aquaculture and restocking, technical fisheries, and the quality of the marine habitat and its biological resources. This mission complies with the provision of scientifically sound policy advice on fisheries, mariculture and the environment, as requested by national and international governmental bodies and professional sectors. The unfavourable economic circumstances (high fuel costs, more rigorous policy measures, and an increasing exploitation of the sea) undermine the fisheries sector thus rapidly that the existence of the Flemish fishery is in danger. On the initiative of the Agriculture and Fisheries Policy Area, a Task Force has been established to work out solutions that can guarantee the liveability of the sector on short, mid and long-term. The initiatives undertaken by the technical fisheries research group of D-VI are focused on energy saving adaptations, reduced by-catches, alternative fishing methods, new target species, quality aspects of landed fish and reconversion. D-VI investigates, in a multidisciplinary approach, how the Belgian fleet composition can be adjusted to a rational and sustainable exploitation model. Besides fisheries, also other anthropogenic activities have a (negative) effect on the marine habitat and especially on marine biodiversity. The studies of the environmental monitoring group of D-VI emphasise the importance of the benthic life in and on the seafloor, because any loss of suitable habitat can induce changes in the diversity and density of the benthos, which leads to changes in the food web structure and to less exploitable fish. These long-term programmes mainly focus on the biological and (bio) chemical effects of dredge dumping, sand and gravel extraction, wind mills and fisheries on the Belgian part of the North Sea. The quality of fisheries products is important to both consumers and producers. Within this theme, D-VI carries out research on the genetic authenticity of fish, shellfish and crustaceans, and on the levels of contaminants in sea fish and fish from the Flemish surface waters (mainly eel). D-VI develops a system to evaluate the freshness, safety and nutritional value of various seafood products. In addition to the accreditation for chemical and biochemical analyses, the implementation of an accreditation system for fish quality and for the assessment of authenticity is worked out in more detail. The national data gathering program in support of the European Common Fisheries Policy is the central theme of the biological fisheries research in D-VI. The research focuses on population dynamics, stock size, discards and exploitation of commercial fish (e.g. sole, plaice, cod and whiting) and crustaceans (mainly langoustines and brown shrimp). Fisheries advice, based on the data thus collected, and the evaluation of the European fisheries advisory process, represent a considerable added value for D-VI. In addition, studies are conducted in the field of aquaculture and restocking, focusing on the cultivation and regeneration of commercial fish species on land (mainly sole, turbot and sea bass), and of commercial shellfish in the open sea (blue mussel). D-VI investigates how wild fish populations can be replenished through the restocking of cultured fish, how cultivation methods can be improved and how the environmental impact from mariculture can be reduced. The genetic research on rotifers as food for larval fish is equally part of this aqua- and mariculture research. 19 Social Sciences Burg. Van Gansberghelaan 109, bus 2 B-9820 Merelbeke Tel.: +32 (0) 9 272 23 40 Fax: +32 (0) 9 272 23 41 L&[email protected] www.ilvo.vlaanderen.be/l&m The new Social Sciences unit was set up in the course of 2006. The scientific part of the former Centre for Agricultural Economics (CAE) is part of ILVO since 1 January 2006. From 1 January 2007 on, part of the staff of the Flemish Policy Research Centre for Sustainable Agriculture will join the unit. Scientific directors In 2006 the research assigned to the CAE was continued, while a vision for the unit’s future was developed (see further in “Extra highlighted”) and new research was started in view of the mission that is put forward for our unit: dr. ir. Ludwig Lauwers ir. Dirk Van Lierde 1.2 Researchers 20 ir. Dakerlia Claeys ing. Anne-Marieke Cools (until 31/03/06) ing. Joost D’Hooghe ir. Lieve De Cock ir. Karen De Mey ir. Sven Defrijn (since 6/11/06) ir. Bruno Fernagut ir. Hadewych Georges (until 31/03/06) ir. Kristof Geutjens (until 31/03/06) ir. Eva Kerselaers ir. Nicole Taragola ir. Jef Van Meensel ir. Anneleen Vandenberghe (until 31/03/06) ir. Hilde Wustenberghs to present and clarify, on a scientific basis, the social choices concerning a sustainable and competitive Flemish agriculture and fisheries. Flemish agriculture and horticulture need to stay competitive, while faced with important economic, technological, institutional, demographic and social developments. Good craftsman-ship no longer suffices. Managerial and entrepreneurial skills have a growing importance. To keep up and improve good entrepreneurship and to meet growing societal demands related to environmental sound production and animal welfare constant innovation is essential. More than other sectors, agriculture and horticulture are allied with the environment, as they work directly with and in nature. Sustainability and corporate social responsibility thus are of vital importance. Therefore the Social Sciences Unit not only works on the economic pillar, but also on the ecological and the social pillar. The fact that the research deals with all social concerns is reflected while naming the unit. Sustainable agriculture needs a multidimensional approach. A holding’s or the sector’s performance needs to be assessed broadly, on the basis of data on all dimensions of sustainable agriculture. In integrated evaluation, the three pillars are weighed against each other. Moreover, attention given to the countryside is growing and the rural area gets attributed a whole range of functions. On the one hand it concerns ‘new’ functions, such as recreation, tourism, biodiversity conservation. On the other hand, the role of agriculture in sustainable rural development remains an important topic. Diversification and multifunctionality are key factors in this matter. Rural development indicators are an interesting tool to gain insight in the specific needs and opportunities of the countryside. In the changing surroundings “What if …?” becomes an often-heard question in the agricultural community. Both policy makers and farmers are wandering about the consequences of certain decisions (changes in subsidies, tradable production rights, stricter environmental regulations, innovative products or production methods, etc.). The Social Sciences Unit is developing simulation models to answer these kinds of questions. Finally, when using models or in innovative research in general, it is very important to have good communication between researchers and their customers (farmers or policy makers). Research is done on the appropriate organisation of knowledge exchange and into the optimisation of the knowledge flow. Research themes 2006 Management and innovation on agricultural and horticultural holdings · Management of glasshouse holdings · Information and Communication Technology (ICT) adoption in horticulture · Radical innovation in agriculture and horticulture: the case of conversion to organic farming Sustainable agriculture: corporate social responsibility · Nutrient and pesticide use: current situation and possibilities for reduction · Study on the socio-economic impact of different housing systems for laying hens Integrated evaluation of economic and environmental aspects · Evaluation of new technologies to improve economic and environmental performance of animal production farms · Ecologically adjusted production analysis models · Space as a meta-dimension over the other functionalities of the environment Rural development and diversification of agriculture · Rural development and policy indicators Models for policy analysis · Farm model to estimate the economic potentiality of conversion from conventional to organic farming · Evaluation of the environmental impact of agriculture with agri-environmental indicators Analysis and optimisation of the knowledge flow between researcher and client · Facilitating knowledge flow in the modelling process · Optimisation and structuring of Flemish research in organic agriculture Service · Spin-off of indicator research to various monitoring assignments, such as the annual Flemish environmen- tal report (chapters in MIRA-T and background documents) and indicator calculations for OECD and EUROSTAT · Advice concerning policy issue · Participation in diverse stakeholder and feedback groups and refereeing of policy preparatory docu- ments 21 Applied Genetics and Breeding Growth and Development Plant Sciences 1.3 22 Caritasstraat 21 B-9090 Melle Tel.: +32 (0) 9 272 29 00 Fax: +32 (0) 9 272 29 01 [email protected] Scientific director dr. ir. Johan Van Huylenbroeck Researchers ing. Wendy Aartsen (until 21/06/06) ir. Joost Baert ing. Evelien Calsyn dr. ir. Els Coart (until 20/10/06) ir. Hervé De Clercq ir. Ellen De Keyser ir. Jelle De Keukeleire dr. ir. Jan De Riek ir. Angelo De Witte ir. Barbara Duquenne (until 30/09/06) dr. ir. Tom Eeckhaut ing. An Ghesquière Msc. Hossein Hosseini dr. ir. Veerle Lamote dr. ir. Leen Leus ing. Marianne Malengier Msc. Lina Maloukh Msc. Khosro Mehdikhanlou dr. ir. Hilde Muylle ir. Georges Rijckaert dr. ir. Isabel Roldan-Ruiz dr. ir. Inge Van Daele ir. Katrien Vandepitte ing. Sabine Van Glabeke dr. ir. Kristiaan Van Laecke ir. Katrijn Van Laere ir. Muriel Vandewalle ir. Gijs Van Ranst dr. Rebecca Zwart 1.3.1 Applied Genetics and Breeding The key research tasks of the unit focus on the generation of new knowledge and policy support in the field of plant genetics and breeding. A multidisciplinary approach and the integration of knowledge from different research domains are essential to achieve the proposed goals. Breeding activities are focused on the development of pre-breeding material. A reduction in the use of chemical pesticides is the basis for a sustainable agriculture and horticulture. Improving the resistance to diseases and pests is a priority in this respect. The selection for improved resistance is among other things based on the natural variation. Diseases of priority for ryegrass and leek are rusts; for clover: Sclerotinia and mildew; for roses: mildew and black spot; for azalea: Phytophtora and Cylindrocladium; for green manure crops: nematode resistance and for wheat: Fusarium head blight. The selection of pest and disease resistance is a combination of a proper knowledge of the pathogen and its interaction with the crop, as well as the availability of efficient screening techniques. This is the basis for the development of DNA-marker assisted selection strategies for ryegrass, wheat and leek. A better use of nitrogen and quality are important points of attention in the creation of new varieties of fodder crops. A higher nitrogen use efficiency by grass and a higher amount of water-soluble carbohydrates leads to smaller N-losses. Clover is an alternative N-source for grassland replacing mineral N-fertilisation. The search for new clover cultivars contributes to the reduction of external N-input in dairy farms and limits N-losses into the environment. In ornamentals, there is a great demand for novelties with clear esthetical benefits. In order to maintain the international position of the Flemish ornamental sector, technological innovations that accelerate or improve the breeding process, are extremely important. Thus, for instance, novel techniques are applied to enable interspecific crosses and/or to circumvent crossing barriers. One of the tasks of the unit is to search for alternative applications of crops. In chicory, we investigate the heritability of inuline chain length, in view of applications in the food and non-food sectors. In hop we try to enhance the biosynthesis of prenylflavonoids. The production of bioactive components with a large economic value in plants can offer important advantages. Furthermore, research is conducted to optimise crops in view of the production of bio-energy. The potential of various woody crops for combustion are studied. Concerning biodiesel, a higher seed production in rapeseed is envisaged using genetic approaches. In the framework of international agreements, maintaining the genetic heritage is an important aspect. For this purpose, gene banks were created for important Flemish agricultural and horticultural crops with the aim of maintaining genetic biodiversity on the one hand and for the exploitation of interesting characteristics in the development of sustainable agriculture and horticulture on the other hand. The application of new developments in biotechnology and bio-informatics in combination with field and glasshouse trials requires a multidisciplinary approach. Close collaboration with other institutes guarantees that optimal results will be obtained. Services It is important that research results are transferred to the sector concerned. Our results are widely disseminated by issuing publications, organising demonstrations and lectures and providing services. Our multidisciplinary and creative approach to breeding contributes to high qualitative seed and starting material for small and alternative crops in Flanders. The benefits of research and selection are also widely distributed internationally. More than one hundred cultivars have plant breeder rights or appear on, at least one variety list. Seed merchants carry out the production of certified seed and the final commercialisation. Cuttings of ornamentals are produced and sold through external companies in collaboration with the azalea innovation fund or Best – Select. In addition to the research activities focused on product improvement and innovation we manage several services for the government and the professional sector. From research the essential expertise is developed for the genetic identification of varieties using molecular markers. These techniques are applied to determine purity, homogeneity 23 and to ascertain whether the cultivar is true to type. Our laboratory is equipped with a flow cytometer to do ploidy analysis specifically adapted to the requirements of third parties (research institutes, universities and companies). Regular contact with different sectors gives valuable feedback about our research, helping to ensure that our efforts are successful. Our unit is involved in EU-framework programmes, R&D projects and the SME-programme of the Flemish government. Research themes 2006 - - - - - - - - - Development of markers for assisted breeding and sustainable agriculture Breeding and molecular genetic research on agricultural and horticultural crops towards the production of biopharmaceutics, functional food and biofuel Alternative induction of polyploidy in ornamentals Improving disease, pest and stress resistance Fast determination methods and DNA-markers for nutritional value and nitrogen use efficiency of Italian ryegrass and grass-clover Genetic diversity of agricultural and horticultural crops and analysis of the potential use of wild types Study of the biodiversity of populations of wild species Pre-breeding and interspecific crosses in diverse crops Research towards optimising seed production techniques Services - - - - 24 Technological advice on applied biotechnology for the ornamental sector Collection and maintenance of the genetic patrimony of fodder crops, open field vegetables and horticultural crops Development of cultivars, production and delivery of starting material Tracing, authenticity control and ploidy analysis. 25 Scientific director dr. Martine Maes Researchers Prof. dr. Bobev Svetoslav (from15/07 until 30/09/06) ir. Casteels Hans ing. Crepel Caroline (until 30/09/06) dr. Cottyn Bart ir. Damme Nicole (since 16/10/06) dr. ir. De Bode Jane (since 01/09/06) ir. De Dobbelare Isabelle dr. De La Peña Eduardo dr. ir. De Wael Lutgart dr. ir. Heungens Kurt ir. Hoedekie Annemie lic. Van Droemme Joachim ing. Van Poucke Kris ir. Van Vaerenbergh Johan ir. Vercauteren Annelies dr. ir. Viaene Nicole lic. Waeyenberge Lieven ir. Wesemael Wim ing. Witters Johan 1.3 Crop Protection Plant Sciences Burg. Van Gansberghelaan 96, bus 2 B-9820 Merelbeke Tel.: +32 (0) 9 272 24 00 Fax: +32 (0) 9 272 24 29 [email protected] 26 1.3.2 Crop Protection There are four disciplines in our Crop Protection research unit: bacteriology, mycology, entomology/acarology and nematology. Our research is targeted on the development of sustainable pest and disease control strategies. The activities can be situated into three domains: 1) development of fast methods for identification and detection of the pathogens, 2) study of the biology and epidemiology of the pests and diseases, and 3) research on alternative pest and disease control methods, such as biological control or the induction of general disease defence through interference with the plant growth process. Artificial inoculation methods are developed to enable the screening of disease resistance levels in plants and the evaluation of control measures. DNAbased methods are developed and routinely applied for identification and detection of specific pests or diseases and for microbial ecology studies. With these methods it is possible to trace the target pathogens in the complex environment of the crops. Specific sampling schemes are developed where needed. The Crop Protection unit also contains a Diagnostic Centre, in which plants and plant products are analysed for the presence of quarantine and regulated pests, and in which disease and pest symptoms as well as non-infectious diseases are diagnosed. The services of the Diagnostic Centre are available to the government, plant breeders, experimental stations and the general public. Indirectly, the Diagnostic Centre also serves a monitoring function for current phytosanitary issues in the sector, and needs for further research can be defined. Research themes 2006 Methods for detection and diagnosis of plant pathogens • Development of molecular diagnostic methods for plant parasitic nematodes, fungi, bacteria and insects • Development and validation of tests for the detection and identification of plant pathogenic bacteria, fungi and nematodes Research on specific pests and diseases • Bacterial diseases of leafy vegetables, with a focus on lettuce and leek • Phytophthora ramorum on Rhododendron and in forestry • Puccinia horiana on Chrysanthemum • Colletotrichum acutatum in strawberry • Xanthomonas fragariae in strawberry • Ecology of Ralstonia solanacearum and Clavibacter michiganensis subsp. sepedonicus • Biology and control of the root knot nematodes Meloidogyne chitwoodi and M. fallax in field production of vegetables • A survey of plant parasitic nematodes in grass fields and cyst nematodes in field cultures • Spider mites in tree-nurseries Disease control • Safe recirculation of nutrient solutions in soilless cultures through effective sterilisation techniques • Enhancement of disease resilience through the use of composts • Inventorising the natural enemies of spider mites in tree nurseries • Inventorising the natural enemies of aphids and the witlof chicory fly in the culture of witlof chicory roots • Scientific support of the warning system for pests in ornamental plants • Optimising the management of insect pests (especially grubs) using entomopathogenic nematodes Explorative basic research in the interface of agriculture and the green environment • Bleeding disease of horse chestnut More expertise • Watermark disease of willows • Interaction between plant parasitic nematodes (Pratylenchus spp.), helm grass and natural nematode antagonists in a dune environment Services • Diagnostic Centre for Plants Artificial inoculation methods for screening of plant resistance and for evaluation of control strategies 27 Crop Husbandry and Environment Plant Sciences 1.3 28 Burg. Van Gansberghelaan 109, bus 1 B-9820 Merelbeke Tel.: +32 (0) 9 272 27 00 Fax: +32 (0) 9 272 27 01 [email protected] www.ilvo.vlaanderen.be/Plant_nl/T&O.htm Scientific director Prof. dr.h.c. dr. ir. Lucien Carlier Researchers dr. ir. Barbara Chaves (since 1/03/06) ir. Sofie Devacht ing. Nadine De Bel (until 31/07/06) dr. ir. Alex De Vliegher ing. Hendrik Engels (until 31/05/06) ir. An Jamart (until 30/06/06) dr. ir. Peter Lootens ing. Bram Marynissen ing. Anja Ritserveldt dr. ir. Bart Vandecasteele (since 1/09/06) ing. Kurt Vandenhecke (since 1/07/06) ing. Walter Van Keirsbulck ing. Chris Van Waes ir. Koen Willekens 1.3.3 Crop Husbandry and Environment The mission of the research domain “Crop Husbandry and Environment” is to explore research activities in order to maintain a sustainable agricultural and horticultural production. The domain examines the impact of agricultural activities on yield and quality and on the environment, in the framework of a multifunctional goal for a sustainable agriculture and horticulture. Caring for quality is a permanent concern and the accreditation of some chemical analyses (crude ash, crude protein, crude fibre, NDF, ADF, ADlignin and starch) according to ISO 17052 is in the final phase. On the social level, there is a permanent concern for a good communication with all the actors: the Government, the producers and the consumers. An annual update of the descriptive and recommended list for agricultural species is edited. The cooperation with the activities of the Agricultural Centre for Forage Crops is very intensive, and advice and support is given. There is an extensive networking on national and international level, covering different EU actions, COST activities, federal and Flemish cooperation programmes with countries in Central and Eastern Europe, etc. The applied fundamental research in crop husbandry pays special attention to the C storage capacity of grasslands and urban ecosystems, the influence of tillage activities on erosion, nitrate leaching, the use of farm-made compost and the influence of a lower input of external production means on yield and quality of agricultural productions and their environment. The further development of organic farming and the co-existence with classic and GMO farming is under investigation. In the frame of the EU Regulation 53/2003, the VCU trials of all agricultural species and the DUS tests of some species for the redaction of the national catalogue of agricultural species are carried out as a mandated activity for the Administration ABKL. For DG SANCO visits to the “comparative trials” for grasses will be organised in 2006-2007. The fundamental ecophysiological research focuses on the photosynthesis of crops (grasses, maize, chicory …) in stress situations. Research is developed for a morphological characterisation of crops and varieties by image analysis. Preparations are made to organise an event in 2007 for ‘75 years of plant breeding research in Merelbeke’ and the 14th EGF symposium in Gent on 3-5 September on the subject ‘Permanent and temporary grassland: plant, environment and economy’. Research themes Crop Husbandry and Environment Sustainable crop production systems • Grass/clover, production and feeding value • N-efficiency of forage crops • Energy crops • Co-existence (GMO, non-GMO, organic farming) • Minimum tillage, soil food web and use of compost. Evaluation of plant species and varieties • Redaction of new criteria in the frame of e.g. changed legislation • Varieties for organic farming. Plant characteristics: morphology, physiology and developing processes • Morphologic characterisation of plant parts via image analyses • Developing screening techniques for cold stress based on plant physiology. Plant quality, water and soil management • Optimising and quantification of C-storage under grassland • Research on chemical, physical and physicochemical and non-destructive analysing techniques for the evaluation of plant material, soil, substrate, manure and water. Services • DUS and VCU research (national and EU variety catalogue for agricultural crops), descriptive and recommended variety list and support of the seed control • Technological Advisory Service FarmCOMPOST • Laboratory: quality/composition of plants, soil, substrate and water • Cooperation Flanders with Central- and Eastern-Europe • Damage on pastures and arable land by wintering geese 29 Technology & Food 1.4 30 Burg. Van Gansberghelaan 115 B-9820 Merelbeke Tel.: +32 (0) 9 272 28 00 Fax: +32 (0) 9 272 28 01 T&[email protected] T&[email protected] Brusselsesteenweg 370 B-9090 Melle Tel.: +32 (0) 9 272 30 00 Fax: +32 (0) 9 272 30 01 T&[email protected] Scientific directors Product Quality and Innovation dr. Marc De Loose Food Safety dr. Marc Heyndrickx Business Unit and Service Centre dr. Lieve Herman Agricultural Engineering Prof. dr. ir. Bart Sonck Researchers Product Quality and Innovation lic. An Braekman dr. Katleen Coudijzer dr. Jan De Block ir. Isabel De Boosere ing. Willy De Ville ir. Sofie De Wispelaere ir. Barbara Duquenne (since 01/10/06) lic. Saskia Leleu (since 13/11/06) lic. Sophie Marchand Msc. Asad Maroufi ing. Martine Merchiers dr. Nina Papazova ir. Karen Smet dr. ir. Isabel Taverniers Researchers Researchers Food Safety Agricultural Engineering dr. Nadine Botteldoorn (until 31/03/06) dr. apr. Els Daeseleire lic. Valerie De Jonghe dr. ir. Koen De Reu ing. Herman De Ridder dr. ir. Hendrik De Ruyck dr. Johan Goris (until 30/06/06) dr. ir. Koen Grijspeerdt dr. ir. Winy Messens ir. Sigrid Ooghe drs. Davy Persoons (since 06/11/06) lic. Bjorn Possé (until 31/01/06) ing. Wim Reybroeck dr. Patsy Scheldeman dr. ir. Els Van Coillie ir. Els Van Pamel (since 01/11/06) ir. Geert Van Royen dr. ir. Geertrui Vlaemynck lic. Hadewig Werbrouck ir. Jeroen Baert ing. Katrien Boussery ir. Pascal Braekman ir. Eva Brusselman ir. Hendrik Cnockaert (until 30/09/06) dr. ir. Peter Demeyer ing. Geert Dhaenens (from 28/08 until 31/10/06) dr. ir. Willy Dierickx dr. Nicoline Geverink (until 30/06/06) ing. Els Goossens ir. Veerle Hostens (until 31/03/06) ir. Nele Lauwers (since 24/07/06) ir. Ludo Maeghe (since 01/07/06) ir. Willem Maertens lic. Griet Nijs (until 30/06/06) ir. David Nuyttens dr. ir. Teunis Bastiaan Rodenburg (until 30/06/06) dr. Frank Tuyttens (until 30/06/06) ir. Jürgen Vangeyte ing. Annelies Van Nuffel lic. Els Van Poucke (until 30/06/06) ir. Stijn Windey 31 1.4.1 Product Quality and Innovation Burg. Van Gansberghelaan 115, bus 2 B-9820 Merelbeke Tel.: +32 (0) 9 272 28 00 Fax: +32 (0) 9 272 28 01 Brusselsesteenweg 370 B-9090 Melle Tel.: +32 (0) 9 272 30 00 Fax: +32 (0) 9 272 30 01 T&[email protected] http://www.ilvo.vlaanderen.be/T&F/Product_quality T&V-PI performs research aimed at improving the functional quality of food as well as the control of the authenticity to better protect the consumer and to improve the market position of the Flemish producer in the framework of durable production and processing methods. For fraud control, methods are tested to determine the species and treatment authenticity and extensive research is carried out concerning genetic modified organisms (GMOs). The last item deals with GMO characterisation and detection, and with the correct implementation of the GMO regulations in the EU, inclusive aspects of co-existence and non-GMO agriculture systems. Chemical-physical and technological research is carried out for innovative functional food products and quality improvements connected with stability, composition, taste, processing and shelf life of dairy products. Product development on the scale of the farm dairy producer is another important aspect. T&V-PI is involved in many bilateral, national and international research projects and its research is based on a multidisciplinary approach and close cooperation with other institutes. The obtained knowledge is valorised by the publication of results and the organisation of seminars and workshops. 32 T&V-PI – Research themes 2006 Authenticity • Methodology development for stability, shelf life and taste of dairy products • Methodology development for authenticity of product composition and processing (milk, milk products, honey) Product quality • Stability of dairy products with long shelf life • Characterisation of psychrotropic Pseudomonas and its heat-resistant enzymes in raw and heat processed milk Product development • Processing and quality of milk with modified milk fat composition (functional food) • Development of functional drinks with health promoting properties • Development of dairy products for dairy farm producers GMOs • • • Integrated approaches for screening and identification of GMOs (including non-approved GMOs) Co-existence and traceability of GMO and non-GMO supply chains Sustainable introduction of GMOs in European agricultural systems 1.4.2 Food Safety Brusselsesteenweg 370 B-9090 Melle Tel.: +32 (0) 9 272 30 00 Fax: +32 (0) 9 272 30 01 T&[email protected] http://www.ilvo.vlaanderen.be/T&F/FS_Food_safety T&V-VV performs research for the improvement of the microbiological and the chemical safety of food products to better protect the consumer and to improve the market position of the Flemish producer in the framework of durable production and processing methods. Food safety is very important for the consumer. Much attention goes into research concerning microbiological safety. Here, molecular identification and typing techniques play an important role. Animal experiments will probably be replaced by in vitro techniques. Research is carried out on the behaviour of zoonotic micro-organisms in the food production chain and the virulence for humans and animals as well as on farm management adaptations reducing pathogens and harmful bacteria. The effect of these different measurements is quantified by risk assessment. Chemical food safety is another important research item. Detection methods for tracing contaminants and residues of veterinary drugs are being developed. New screening methods are tested and chromatographic methods are developed for confirmation. The latter will assist in tracing the contamination source and remediation suggestions will be formulated. The reduction of problems with veterinary drugs at farm level receives much attention and the influence of poultry housing systems on chemical contaminants is also being studied. T&V-VV – Research themes 2006 Microbiological safety of food • Detection, identification and typing of new microbiological risks as Mycobacterium avium subsp. paratuberculosis, pathogenic viruses and shigatoxin-producing Escherichia coli and moulds • In vitro techniques (cell cultures, fermentation) for the study of the interaction of the pathogen-host relation • Differentiation of pathogens based on virulence towards animals and humans and influence of sublethal stress on virulence • Presence of pathogens and spore-forming flora on the farm • Influence of housing systems on the presence of pathogens • Control of the contamination risk of broiler flocks and pigs with Campylobacter and Salmonella • Bacteriological contamination and infection of shell eggs in the production chain Chemical safety of food • Sample preparation based on new technology as “Molecularly Imprinted Polymers” • Pyramid structure for detection of coccidiostatics in eggs • New screening methods for antibiotics • Study of the source of veterinary drug residues in food products and migration of veterinary drugs in honey T&V-VV is involved in many bilateral, national and international research projects and its research is based on a multidisciplinary approach and close cooperation with other institutes. The obtained knowledge is valorised by the publication of results and the organisation of seminars and workshops. 33 1.4.3 Business Unit and Service Centre Brusselsesteenweg 370 B-9090 Melle Tel.: +32 (0) 9 272 30 00 Fax: +32 (0) 9 272 30 01 T&[email protected] http://www.ilvo.vlaanderen.be/T&F/Business_unit In T&V-BU very important activities concern reference working and consultancy towards the government, laboratories, dairy industries, small and medium enterprises, farmers, etc. For this, ring trials (for the dairy sector, spray nozzle manufacturers, etc.) are organised and consultancy is concentrated in dedicated Technological Advisory Services. For the service towards private companies, T&V-BU is equipped with accredited laboratories, test halls, a renewed workshop and a pilot dairy plant. In this pilot plant equipment is available for extrusion and production of cheese, milk powder, drinks, desserts and ice cream and this on a semi-industrial scale. This equipment is used in technological research projects and in private research for the food industry. The test halls can be used for technological research into agricultural engineering. The machine shop is equipped to construct specialised experimental set-ups. T&V-BU – Themes 2006 Reference working and consultancy • Reference working on analyses for food authenticity and food safety (including GMO detection and platform for food safety and GMOs) • Reference working for the “Melkcontrolecentrum- Vlaanderen” • Consultancy for the governments on authenticity (including GMOs), food safety and food quality • Expertise in evaluation and Flemish authorisation of food quality regulations • Spray techniques: Accredited inspection of sprayers and accredited laboratory for spray technology • TAD Hoevezuivel ( Farm Dairying): New practices and technologies on dairy farms • TAD Agriconstruct: Construction and renovation of buildings and materials in agriculture and horticulture • TAD Agromech: Mechanisation in agriculture and horticulture. 34 Public service • Laboratory analyses concerning food authenticity and food safety (including GMO analyses) • Technological trials for feed and food transformation • Technological experiments on agricultural engineering • Control: Management of approved dairy technicians • Preventagri Vorming: Safety and health of workers in agriculture and horticulture. 1.4.4 Agricultural Engineering Burg. Van Gansberghelaan 115, bus 1 B-9820 Merelbeke Tel.: +32 (0) 9 272 28 00 Fax: +32 (0) 9 272 28 01 T&[email protected] http://www.ilvo.vlaanderen.be/T&F/AT_Engineering The Agricultural Engineering Research Group carries out applied scientific research and gives advice and services on the development of advanced techniques, the integration and evaluation of new and existing techniques for innovative animal and environment-friendly agricultural and horticultural production systems. Besides the conventional technical competences (like mechanics, study of the strength of materials, electro mechanics, pneumatics, hydraulics, measuring – steering – controlling techniques, sensor techniques, etc.), modern mathematical and IT competences are used, such as image analysis techniques, simulation models, data-based modelling, computer fluid dynamic models, computer aided design programs, etc. The applied scientific research on agricultural engineering focuses specifically on the following research topics: • Livestock Precision Farming: development of au- tomatic systems for measuring, monitoring and management of the interactions animal-human- environment • Farm Buildings: design and optimisation of farm building constructions applying advanced techno logies; optimisation of animal house equipment and climate according to the requirements of the farmer and the farm animals • • • • Labour Organisation, safety and farm management: improvement in production systems with a view to an efficient and effective labour organisation, safety and management on agricultural farms and horticultural enterprises Environmental Protection: research on the impact of agricultural activities on the environment and research on environment-friendly production systems Agro mechanisation: the development of measuring techniques, procedures and equipment to determine the technical characteristics of agricultural and horticultural machinery. New techno logies are applied for the optimisation of the performance of these machines, for improving the quality of the products and for the protection of the environment Harvesting, post-harvesting, storage and processing technology: the development, the optimisation and evaluation of harvesting and post-harvesting machines. The study of storage and processing of agricultural and horticultural products will be carried out in collaboration with the Product Quality and Innovation Research Group. The main target is to maximise the quality of the harvested, stored and processed products. T&V-AT-Research themes 2006 Techniques for sustainable animal husbandry • Sustainable material use for agricultural construc tions • Continuous monitoring of milking systems • Development of measuring techniques for the de termination of group and animal-related indices (e.g. body dimensions and conformation of farm animals) • Research into the sustainability of elements for construction, equipment and enrichment of animal houses • Automatic detection of lameness with cattle • • • • Group housing of sows Evaluation of the housing of poultry vis-à-vis animal welfare and animal health Cooperation with the development of a European standard for monitoring animal welfare Animal-friendly alternatives for castrating male piglets and other mutilations Environmental technology • Study and prevention of dust emissions in agriculture • Ammonia emission reduction in animal husbandry using new housing concepts • Spreading techniques for animal slurries and mineral fertilisers (slurry injection, rotary disc spreaders for artificial fertilisers) • Reduction of dust and ammonia emissions Sustainable agricultural mechanisation and post-harvest technology • Optimisation of spraying technology with field, orchard and lance sprayers • Harvest, post-harvest and conservation techniques of crops and vegetables • Reduction in non-target contamination by crop protection products (drift reduction with field sprayers, optimisation of spraying techniques in horticulture) • Measuring procedure for orchard sprayers and lance sprayers • Agro textiles and membranes • Soil improvement and erosion control Measuring and encouraging farm animal welfare. The research domain Agricultural Engineering assisted in the following projects that were transferred during the course of 2006 to the research domain Cattle Husbandry and Animal Welfare – Animal Science Unit: • Research on the effects of animal house enrichment on the welfare of farm animals • Research into indicators for animal welfare (fluctuating asymmetry amongst others) 35 36 2. Important research results 2.1.1 Milk urea concentration as an indicator of the N excretion of dairy cows into the environment Animal Sciences Results of recent trials raise questions on the reliability of MUC as sole parameter to estimate N excretion. In a feeding trial, two basal rations with respectively 100% prewilted grass silage (PGS) and 100% maize silage (MS) were compared. Diets were supplemented to achieve for each cow 105% of its energy and protein requirements. MUC was significantly different, viz. 146 mg/l for 100% PGS versus 217 mg/l for 100% MS. Predictions based on these values will result in strongly different N excretions, whereas one would expect similar N excretions from rations with the same nutrient supply. This was confirmed by balance trials with the same two diets. N excretion was almost identical, 392 and 389 g/day for respectively 100% PGS and 100% MS, but again MUC was clearly higher for MS (252 mg/l) than for PGS (168 mg/l). This discrepancy may be due to differences in urine production, caused by the different mineral contents of the diets. Therefore, a trial was set up with 18 Holstein cows where a maize silage ration was supplemented with either 380 g potassium chloride (KCl) or 510 g sodium chloride (NaCl), in order to obtain comparable Na and K contents as in PGS diets. Results indicated that NaCl and KCl decreased MUC with 22% and 10%, respectively. This confirms the need for further research to find one or more additional milk parameters that improve the estimation of N excretion. 2.1 Previous research showed that milk urea content (MUC) is related to the nitrogen (N) excretion of dairy cattle into the environment, more particularly that through urine. On the other hand, it appeared that the N excretion through faeces is strongly correlated with milk production. Based on balance trials a model was developed at the Animal Science Unit to predict total N output through faeces and urine from milk production and MUC. However, there were indications that the model was less accurate for rations deviating from a classic basal ration, consisting of about half maize silage and half prewilted grass silage on dry matter basis. 37 2.1.2 Reducing ammonia emissions from pig houses through feeding strategy Animal husbandry is responsible for 95% of the ammonia emissions in Flanders, 55% of which originates from pig husbandry. According to a European directive (2001/81 EC) the ammonia emission in Belgium has to be reduced with 13% in 2010, relative to 1990, which means a reduction of 40% for Flanders. Twenty-five percent less ammonia emission needs to be realised with low emission spreading of manure and a further reduction with 15% by low emission housing concepts, feed adaptations and other measures. Additionally, the IPPC directive (Integrated Prevention and Pollution Control, directive 96/61/ EG) needs to be fulfilled for farms with more than 2,000 fattening pig places or 750 sow places in 2007. The issuing of a (new) exploitation permit will include among other things emission norms for ammonia. These norms will be based on the European list of Best Available Techniques. Hitherto, the government only takes into account the ammonia emission reductions obtained with adapted manure spreading and storage techniques and by constructional aspects. From the farmers building new pig houses important (financial) efforts are required to build low emission animal houses, while farmers with existing infrastructure remain (temporarily) undisturbed. However, 38 each pig farmer could help to cut down the ammonia emission by non-constructional measures such as through nutrition. Different feeding strategies are currently tested on a pig farm, in cooperation with the Technology and Food Unit. A first strategy is the use of diets with a reduced protein level that meet the daily requirements for ileal digestible amino acids, by supplementation with synthetic amino acids. It has repeatedly been proven that reduced protein diets diminish the nitrogen excretion without impairing animal performances. However, quantitative data concerning the resulting ammonia emission and its reduction in practice are still missing and will be generated in this project. Furthermore, four feed additives are tested which have already demonstrated either in the laboratory or under experimental conditions that they can exert a beneficial effect on the ammonia emission by different mechanisms. Benzoic acid leads to a lowered urinary pH; enzymes result in a better digestion; clay minerals bind ammonia; and saponins inhibit urease activity responsible for the production of ammonia from manure. From the first results under practical circumstances the tested additives seem capable of reducing the ammonia emission with 13 to 24%. However, it is important to confirm these results with the currently running replicates. 2.1.3 Research into alternatives for antimicrobial feed additives Livestock performance and feed efficiency are closely interrelated with the intestinal health status as a basis for the general health. At intestinal level there is a three-way interaction between (1) the host as characterised by its intestinal morphology, its digestion physiology and its immunity, (2) the microbial load and mutual balance and (3) dietary characteristics such as nutrients and feed additives (see figure). Antimicrobial feed additives have made a tremendous contribution to the profitability of intensive husbandry and provided people with healthy and nutritious poultry products. As a consequence of the increasing concern about the potential for antibiotic resistant strains of bacteria, the European Commission decided by invoking the precautionary principle to ban all commonly used feed antibiotics from January 2006 onwards. Besides adapted management practices, there are a number of non-therapeutic alternatives, including enzymes, (in)organic acids, probiotics, prebiotics, herbs and ethereal oils, immuno-stimulants. Exogenous enzymes improve ileal digestibility, thereby limiting indirectly overloading of the microbial flora. The impact of organic acids on the microbial flora depends on the chemical characteristics of the various acids. The probiotic potential of microbial strains depends on their genetic stability and intestinal metabolism and colonising capacity. Prebiotics either as ‘non-digestible feed ingredients’ or as alternative binding sites have some selective effects on the intestinal microflora. The challenge for using herbs and ethereal oils is to relate their anti-microbial activity to some specific components. Some nutritional factors can modulate (in)directly the immuno-reactivity at the intestinal associated lymphoid tissue, providing protection against bacterial adhesion. There might be additional effects by specific combinations of these additives. Other on-farm management practices may be incorporated into a multifaceted intervention programme “biosecurity” that could limit the transfer and colonisation of pathogens. It must be emphasised that some of these strategies are only measures that will help to partially compensate, but not replace, antimicrobial growth promoters, and will work through ‘indirect’ mechanisms. There is, however, no clear view on the ultimate net cost of the ban on zootechnical performance, morbidity and mortality, slaughter-yield and condemnations and return-on-investment for these alternatives. Any additional cost may be, at least partially, offset by the benefits of increased consumer confidence. Research at the Animal Science Unit on broilers and slaughter pigs, however, shows that there is no alternative currently available which is as efficient and consistent as the antimicrobial feed additives. Moreover, there are still many questions concerning the action mechanisms and the circumstances that are required to maximise their effectiveness. More research is needed to establish the interaction between feed additive and diet characteristics and the effect of combinations of different feed additives. Macrostructure of the intestinal epithelium 39 2.1.4 Qualitative feed restriction in beef cows Foreign and also own research has shown that beef cows can be temporarily fed below their energy requirements. Although double-muscled cows have a lower body fat content compared to other cattle breeds, they can mobilise some reserve tissue. Body reserves are replenished without any negative consequences on body weight change, calving interval and milk yield of the cows, and birth weight, development and mortality of the offspring, when a moderate energy restriction is followed by a period where a good diet is fed to appetite. In the first experiment cows were confined in tie stalls and fed a restricted maize silage based diet. However, the practical application of such a feeding system may be difficult. Double-muscled cows are mostly kept in loosehouses, because this is less time-consuming and needs lower investments for housing. When fed in group, some animals with a higher social order consume too much, so restricting severely the feed intake of the other cows. Moreover, this is bad for animal welfare. The intention of the second experiment was to enable a reduction of the dietary energy density, so that an ad libitum intake would still result in a restricted energy intake. Ten non-lactating Belgian Blue double-muscled cows were fed initially a basal diet consisting of 80% maize silage and 20% wheat straw. Animals were not pregnant or less than 3 months. Parity averaged 1.9. Every four weeks the amount of maize silage was reduced by 20%units, while the amount of straw was increased by 20% 40 up to 80%. The basal diet was supplemented with 0.5 kg of a mineral-vitamin premix and a variable amount of protected soybean meal and urea to maintain the dietary concentration of the protein values DVE and OEB. The diet was prepared with a self-feeding wagon. Net energy value for lactation of the diet decreased from 5.74 to 3.54 MJ per kg dry matter (DM) as a consequence of the increasing inclusion rate of straw. Moreover, intake was reduced by up to 25% with increasing level of straw. Energy requirements for maintenance, as applied for dairy cows, were met for 84% when the diet containing 80% straw was fed. This study showed that the coefficient of variation for daily DM and net energy intake was relatively low, ranging between 8 and 11.5% for the consecutive sub-periods. More than 80% of the variation in daily DM and net energy intake could be explained by the net energy content in the DM of the diet, or the concentration of crude fibre or NDF. From these results, we can conclude that poor quality roughages can be utilised by doublemuscled dry cows or during the first months of gestation, knowing that these animals are able to build up their body reserves during the grazing period. The combination of maize silage and straw may be replaced by hay from an agri-environmental management or from grass seed production. 2.1.5 Early and reliable detection of boar taint Animal welfare is gaining more importance in livestock production. Questions have been raised therefore on the ethical aspects of male piglets castration. Nevertheless, castration is necessary to prevent the occurrence of boar taint. Boar taint is a distinct and unpleasant odour, flavour or taste that can be present in pork products and is revealed when the fat fraction in meat is heated. The production of boar taint is influenced by the male sex hormones, and therefore castration is an effective way to prevent the occurrence of boar taint. As boar taint is unwanted by the consumers, farmers have to take measures. Research is performed in the Animal Science Unit to get rid of boar taint without castration. Changing the diet may influence the occurrence of boar taint. Besides, the project looks at the influence of slaughter age in different pig breeds on boar taint production. Finally, the effect of hygiene in the finishing house on the prevalence of boar taint is studied. The effect of the different treatments is tested by laboratory analyses of the meat and the fat as well as by taste panels. The taste and flavour of the meat and the fat is judged by an expert panel, with members trained for the detection of boar taint, as well as by a panel with unbiased consumers. It is expected that the prevalence of boar taint can be diminished by proper feeding, pig breed choice, adapted slaughter weight and hygienic measures. Totally avoiding boar taint with these measures seems unrealistic, and therefore it is necessary to find a way to detect boar taint on living animals as well as on pig carcasses. If a reliable detection of animals with boar taint is possible, it might be sufficient to take these animals out of the normal production line, and minimise the losses in this way. Therefore, this research project will evaluate whether or not physiological parameters and behaviour can predict whether an animal will develop boar taint. Besides, techniques like the use of an e-nose or a soldering iron will be tested for a rapid detection of boar taint in the slaughter line. By diminishing the prevalence of boar taint on the one hand, and by a rapid and reliable detection of the animals still producing boar taint on the other hand, we hope to find alternatives for castration. 41 2.1.6 Integration of animal welfare in the food quality chain Animal welfare is of considerable importance to European consumers. Nowadays, food quality is not only determined by the overall nature and safety of the end product but also by the perceived welfare status of the animals from which the food is produced. A European standard for farm animal welfare could contribute to informing consumers about the production systems, and improve farm animal welfare. The fact that improving the animal’s welfare can positively affect product quality, pathology and disease resistance also has a direct bearing on food quality and safety. One of the aims of the project is to develop an integrated standardised methodology for the assessment of welfare in cattle, pigs and poultry from farm to slaughter, based on animal measures. First step is defining a list of measures (from existing knowledge) that could be used under practical conditions - from farm to slaughter - and that cover the different dimensions of welfare. Next, the validity, repeatability and on-farm feasibility of the measures will be checked. A comprehensive assessment of animal welfare will then be built by integrating the validated measures. This protocol will be tested and validated. The EU funds the Welfare Quality project: a project designed to develop European standards for on-farm welfare assessment and product information systems. The project aims to accommodate societal concerns and market demands, to develop reliable on-farm monitoring systems, product information systems, and practical species-specific strategies to improve animal welfare. The project focuses on three main species and their products: cattle (beef and dairy), pigs, and poultry (broiler chickens and laying hens). Thirty-nine institutes and universities (representing 13 European countries) with specialist expertise participate in this integrated research project. The project started in May 2004 and will take five years to complete. The Animal Science Unit is involved in developing the protocol for pigs. We tested the validity of a lameness score as a parameter for monitoring the welfare of sows and fattening pigs on-farm and at slaughter. This research was conducted together with the Zootechnical Centre, Catholic University of Leuven and IRTA (Monells, Spain). Furthermore, we will be involved in integrating all measurements done on pigs in a draft protocol. Guilts in group housing 42 43 2.1.7 Value creation in Belgian sea fisheries From an economic point of view, the situation of the Belgian sea fishery fleet is clearly unfavourable. According to the industry, the main causes are the high fuel and material costs. The most heard solutions given by the industry are higher quota or subsidising fuel costs (or a combination of both). However, the solution is not as univocal and simple. Subsidising a variable cost like fuel and raising the fishing quota are not sustainable solutions. In other words, it is necessary to search for real long-term solutions for the Belgian fleet by conducting a thorough economic study, focusing on the cost-effectiveness of the fleet and taking into consideration ecological and social issues. ILVO-Fisheries, in collaboration with the faculty of Economics and Business Administration and the Faculty of Bioscience Engineering of Ghent University, has started this determines the value creation strategy that is decisive for fishermen or ship owners. How the value of the captured fish can be ‘silvered’ through selling is no part of the value creation strategy. This study fits in a broader project to develop a long-term strategy for a sustainable Belgian sea fishery. The four cornerstones supporting this strategy are: the profitability of the fleet, the ecological integrity of the marine ecosystem, the application of modern fishery technologies and attention for education and the social life of fishermen. The study of value creation strategies will form the basis of the economic component of the long-term strategy developed by ILVO-Fisheries. The surplus value of this economic study lies in the combination of the two main objectives: How sustainable is the present “industry concept” for fisheries? exercise under the denominator of “value creation strategies” in the Belgian sea fisheries by using fleet dynamics as a tool. In the context of the Belgian sea fisheries, “value creation” means catching fish, but many kinds of fish can be caught in different ways and on a variety of places (the “strategy”). The combination of strategic choices 44 1) the development of a value creation strategy by using fleet dynamics to make the primary sea fishery industry economically sustainable. Several variations of the value creation strategy will be tested by means of a simulation model of the sea fishery. The model will include economic data, costs, revenues and 2) operational characteristics of different fishing methods; the examination of how different stakeholders (industry, administration, science, policy) learn from the solutions that are handed by the simulation models. The final goal is to stipulate how policymakers can ensure the acceptance of the selected value creation strategies by the primary sector. In a first step, the current ‘industry recipe’ (how the fleet operates now) will be mapped to determine its sustainable value creation. This means that ILVO-Fisheries will investigate how the current fleet is structured, and what economic dynamics are active nowadays. Through a simulation model of the fleet activities, the sustainability of its value creation will be evaluated. In a second step, alternative value creation strategies will be examined through the simulation model. These strategies question the current industry recipe, and take into account new fleet structures (with their economic parameters), other vessel types and alternative fishing methods. Again, the main issue is the sustainability of their value creation for the Belgian sea fishery. The comparison of the different strategies should deliver the best solution to reform the industry in a structural way, aiming at a sustainable fishery. However, the selection of the best option does not automatically lead to its implementation. This raises the second research question: to what extent can this type of research contribute to an effective change in the operational management and attitude of the fishing industry? The project investigates how the presented alternative strategies - developed in the simulation model - can be adopted by the industry. Because of its rather traditional and conservative attitude towards changes, the sea fishing industry is a good target group for this kind of studies. To conclude, it can be stated that, besides recognising the economic problems of the sector, the overall aim of ILVOFisheries is to get a clear view on its value creation strategies, with the intention to guide the industry to a more sustainable sea fishery. 45 2.1.8 Biological fisheries research: there is more to it than just counting fish The management measures in support of the European Common Fisheries Policy (CFP) are based on scientific advice that is accomplished through interaction between different advisory bodies. Key players in this process are the International Council for the Exploration of the Sea (ICES, with headquarters in Copenhagen) and the European Commission’s Scientific, Technical and Economic Committee on Fisheries (STECF). ICES was established in 1902, to promote marine research in the North-East Atlantic Ocean and to coordinate the research activities of its member states (20 at present, amongst which Belgium). In the beginning, ICES was a mostly scientific forum with emphasis on fundamental fisheries research, but for some decades now, it has become the major fisheries advisory body for the North-East Atlantic. Each year, ICES issues management advice for over 150 commercial fish and shellfish stocks upon request of, amongst others, the European Commission and the North-East Atlantic Fisheries Council (NEAFC). ICES’ management proposals and the associated catch predictions are based on hundreds of thousands of data collected by its member states (amongst others through market and atsea sampling, and fishery-independent stock surveys with research vessels) and on the use of analytical and predictive models which have largely proven their dependability. In addition, the elaboration of the advice is subjected to strict rules, governed by the principles of the precautionary approach. For its advisory tasks, ICES can rely on a multitude of specialised expert groups, which can roughly be sub-divided into two groups: the permanent, regional assessment working groups on the one hand (stock evaluations and catch predictions) and the supporting, methodological working groups on the other hand (refinement of evaluation techniques). ILVO-Fisheries is actively involved in about ten of these groups, including the Working Group on the Assessment of Demersal Stocks in the North Sea and Skagerrak, the Working Group on the Assessment of Northern Shelf Demersal Stocks (with responsibilities over, amongst others, the Irish Sea) and the Working Group on the Assessment of Southern Shelf Demersal Stocks (with responsibilities over, amongst others, the Celtic Sea). Before ICES releases its fisheries advice (in November), the reports of the assessment working groups are critically evaluated by the Advisory Committee on Fishery Management (ACFM), the highest advisory body on fishery-related 46 Plenary meeting of the STECF Sub-group on Research Needs, presided issues within ICES. At the meetings of the ACFM (which are regularly attended by experts from ILVO-Fisheries), the outcomes of the working groups are checked for quality and mutual consistency, and the advice is compared to the most recent management rules laid down by the endusers (European Commission, NEAFC, etc.). Once released, the management advice goes to the client groups, who then translate the scientific advice into TAC proposals (Total Allowable Catches) in the interest of, for example, the European Council of Ministers. As the geographical and thematic responsibilities of ICES are limited, a second and at least equally important advisory body has been established within the European Commission, viz. the Scientific, Technical and Economic Committee on Fisheries (STECF). The STECF focuses on, amongst others, the mixed fisheries advice (the so-called multi-fleet and multi-species approach to fisheries management) and the economic aspects of the European conservation measures. To that aim, the STECF can count on a number of permanent expert groups (such as the Sub-group on Review of Stock Status and the Sub-group on Economic Affairs), together with a varying number of ad hoc study groups which are convened to advise on specific problems (e.g. the recovery plans for cod, the economic impact of a series of alternative management and especially the smaller member states have difficulties to meet the tight quality requirements. The consequence being, that harmonisation, cooperation and task-sharing have become the new creed to the data collectors. Co-operation in the broad field of data collection is a complex issue, with many players involved. ICES, for example, has dedicated working groups who take care of the planning of the international sea-surveys (such as the Working Group on Beam Trawl Surveys and the International Bottom Trawl Survey Working Group) or the methodological improvement of data collection (such as the Planning Group on Commercial Catch, Discards and Biological Sampling, PGCCDBS). PGCCDBS also organises workshops and ring tests (amongst others on the ageing of fish and the identification of maturity stages) that help standardising the analytical methods used. d by Frank Redant (ILVO-D-VI) plans for plaice, etc.). Also at this level, ILVO-Fisheries is strongly involved, with participants to advisory groups on a wide variety of topics. It goes without saying that international cooperation is a prerequisite to the stock evaluations and the elaboration of management proposals. The vast majority of fish stocks in the European waters are fished by different countries and thus, the merger of national data to international datasets is an essential first step in the process. One of the results being, that international cooperation has become a second nature to assessment biologists. Over the past years however, international cooperation has become increasingly important at a lower level too, viz. at the level of the policy supporting data collection itself. Since 2002, data collection in support of the CFP is subject to a number of EU Regulations (Council Regulation 1543-2000 and Commission Regulations 16392001 and 1581-2004), commonly known as the Data Collection Regulation (DCR). The DCR puts heavy duties onto the member states, with its far-reaching obligations to collect extensive biological and economic data on their national fisheries and fishery-related activities. The stakes are high (which is perfectly defendable, as unreliable or incomplete data are of no use to the stock evaluations) At the regional level, cooperation between the data collectors is ensured through the Regional Coordination Meetings (RCMs), where the task allocation with regard to biological data collection (which member states do what, where and when) is discussed, not only with a view to harmonising data collection but also to make the process more cost- effective. For the moment, there are seven RCMs, viz. for the Baltic Sea, the North Sea, the NorthEast Atlantic, the North-West Atlantic, the Central Atlantic, the Mediterranean and the highly migratory tuna species. Staff members of ILVO-Fisheries take part in two RCMs, viz. the one for the North Sea and the one for the NorthEast Atlantic. Finally, at the level of the European Commission, there is the STECF Sub-group on Research Needs (SGRN), which has an important controlling and advisory task in the fields of biological and economic data collection, on the Commission’s and the member states’ behalf. The SGRN is responsible for the follow-up and the evaluation of the member states’ national data collection programmes and for the triennial revisions of the DCR. Also in this area, ILVO-Fisheries plays a prominent role, with attendants to both the half-yearly plenary meetings of the SGRN and the ad hoc workshops on the revision of the DCR. 47 2.2.1 Modelling the agricultural sector 2.2 Social Sciences For a long time, agricultural policy was focused on adjusting the aggregated outcome of individual farm decisions concerning the supply of commodities and the demand for production factors, in which the game of demand and supply resulted in an equilibrium. Policy intervention was restricted to market and price corrections to safeguard farmer’s income. Econometric sector models were reliable instruments for ex ante evaluation of the impact of these interventions. 48 Over the last decade however, agricultural policy has been changing drastically. With environmental and sustainability concerns entering the public debate, new types of policy instruments emerged. In addition, under pressure of several international negotiation rounds, farmers are increasingly confronted with trade liberalisation: price and production linked intervention decreased and were partly replaced by direct income and rural development support, production rights and other instruments. Instead of the average response of the sector, changes in individual farms’ activity mix and the interactions between different farms become more and more important. Even when policy is organised at a supra national level, outcomes highly differ according to farm type and farm location. The use of farm level models, in contrast with sector models, generates an added value with respect to this evolution. Such models offer more insight in the effectiveness of specific policy measures, taking into account as much farm specific factors as possible. The information obtained can be applied for purposes such as diagnosis and recommendations for farmers, the supplying and processing sectors and policy-makers. Moreover, due to the ever-increasing power of computer processors, the simulation techniques are much improved. As a consequence, models are no longer restricted to the aggregated optimisation of supply and demand anymore. The SEPALE-model (Dutch acronym: Systeem ter Evaluatie van de Politiek aangaande Landbouw en Leefmilieu, system for evaluation of the agricultural and environmental policy) has been developed during an IWT project which ended at the beginning of 2006. The model is based on assumptions of the economic and production-technical choice behaviour of the farmer, with data from arable, cattle and dairy farms of the FADN data network of Flanders. SEPALE is a farm level model. According to the context in which the policy simulations are desired, farms can be selected on specific characteristics such as economic size, geographical position and technical orientation. This flexibility enables to run simulations for certain farm types and regions separately. Although SEPALE is not an aggregate supply model like other well- known models (CAPRI, SELES, etc.), the modelling technique of SEPALE relies on PMP (Positive Mathematical Programming) as well. This modelling technique warrants a reliable reproduction of the supply behaviour of farmers, based on the production behaviour of those farmers. Through farm level optimisation, besides price shocks, quota restrictions, subsidy and income effects, SEPALE also can simulate internal exchange of production rights and factors. Even though not the entire selection of Flemish farms is included in the model, it nevertheless can be considered as a supply model, since aggregating the farms provides a representative picture of the sector. In a first series of model applications, the impact of the Mid-Term Review, the Sugar Reform and a set of rape seed applications for biofuel production were analysed by simulations of the model. The Sugar Reform was an ideal policy case because it enclosed four main policy instruments: intervention price reduction, quota reduction, intervention price compensation and coupling of the compensation. Insight in this complex matter of policy instruments was facilitated by running model simulations. The results showed amongst others that only a quota reduction would have a strong effect on the supply of sugar beets (figure 1). and their farm gate surplus. By integrating a production function, substitution between manure, fertilisation and concentrate use by increasing manuring limits were simulated. The results showed that the environmental pressure (measured as farm gate N surplus) decreases at first, but from a critical threshold on, starts to increase again, in spite of stricter manuring limits. (figure 2). Figure 2: Impact of the manuring limits on the N farm gate surplus of dairy farms. At the end of 2006, a new IWT-project started: Price creation and allocation of rights in agriculture and horticulture. A quantitative descriptive system of all kinds of exchangeable rights will be developed, based on the SEPALE model. Figure 1: Effect of price and quota reduction on supply of sugar beets The most recent developments concerned the integration of environmental indicators related to nitrogen. For arable, cattle and dairy farms, the most important components of the nitrogen flow were incorporated in the model. This enabled simulating the effect of policy instruments on farm gate surplus, split up into soil balance and ammonia emission. Starting from the N flows in the farm gate surplus and the cattle’s feed requirements, more dynamic manure excretion coefficients were estimated. Also, scenarios with respect to the manure policy could be simulated. For this, the dairy farms in the model were divided into four groups, according to their degree of manuring 49 2.2.2 Corporate social responsibility (CSR) As in other economic sectors corporate social responsibility has become an important issue in farm and horticultural business management. In socially responsible or sustainable business management economic results, social and environmental concerns, or the three P’s: “Profit”, “People” and “Planet” are integrated in the business operations. Corporate social responsibility implies that the business manager or farmer takes up responsibilities that go beyond the strictly economic responsibility for his/her business or farm. Research on corporate social responsibility of Flemish farmers and horticultural entrepreneurs is actually rather scarce. The Social Sciences unit tries to meet this problem, as is demonstrated in some recent research projects. As an illustration, two projects of the past research period are highlighted. Both projects were realised for the Department of Agriculture and Fisheries and carried out in close cooperation with stakeholders within the sector and researchers from other institutions. Nutrient and pesticide use in Flemish horticulture: current situation and possibilities for reduction In the first research the current situation on the use of nutrients and pesticides in Flemish horticulture and the possibilities for reduction were studied. This research was done in collaboration with four research centres in Flanders: the research centre of Hoogstraten, the research centre for vegetables of Kruishoutem, the research centre for ornamental plants in Destelbergen and the research centre for fruit in Gorsem. The four centres provided technical expertise for each of the four subsectors of the horticultural sector: greenhouse vegetables, open air vegetables, ornamental plants and fruit. For each of the four subsectors a committee of experts was created, made up of about 15 experts in plant protection and nutrient use per subsector. The same research method was used in each of the four subsectors. First of all an inventory was made of the use of plant protection products and nutrients in Flemish horticulture. The data used were taken from the Flemish horticultural accountancy data network, completed with data from special inquiries. The second, and most important part of the research, made an inventory and evaluation 50 of reduction techniques for nutrients and plant protection products. For each of the four subsectors an inventory was made. From the list of possible reduction techniques in every subsector ten promising techniques were selected. There were seven reduction techniques for plant protection products and three for nutrients. The aim of the research was to make up an accurate image of the actual potential of each technique to reduce the environmental impact for each subsector. The research had to give concrete information on the environmental impact that can be realised on the one hand and the possible impact on the economic, technical and organisational aspects on farm level on the other hand. The collected information allowed an evaluation of the techniques according to their potential to reduce the environmental impact. To collect this information two methods were used: first of all expert groups tried to obtain an insight in the feasibility of each technique and the limiting conditions and thresholds for adoption on farm level, secondly a detailed survey was performed at horticultural holdings in order to identify the perception of horticultural entrepreneurs with regard to the proposed techniques. We found that there are still possibilities to reduce the use of plant protection products and nutrients. Many entrepreneurs did know the proposed techniques, and some of them already introduced them or intended to introduce them at their holding. It appeared that there is a need for demonstrations of these techniques and that the horticultural entrepreneurs are in favour of more research and more information on environment-friendly production. If these demonstrations can prove that these techniques have no negative impact on the economic results of the holding, most horticultural entrepreneurs are willing to apply them and to adapt their management. In this way it is possible to produce in a more socially responsible way. The research demonstrated that a more socially responsible farm management could be applied when it fits within the economic objectives of the horticultural holding. Study about the socio-economic impact of different housing systems for laying hens A second research project dealing with socially responsible farm management was a study about the socio-economic impact of different housing systems for laying hens. This research was done in collaboration with the Animal Science Unit of ILVO and the Provincial Centre for Practical Applied Poultry Research in Geel. The study was carried out to examine the impact of EU-Directive 99/74/EU, which bans conventional cages for laying hens from 1 January 2012. From 2012 on only furnished cages and alternatives - floor housing and aviary systems - will be allowed. First of all a literature review was made to work out the qualitative and quantitative consequences of the different systems on the production results of laying hens. Consequences in the economic field (costs of investments, fixed and variable costs, profitability, etc.), consequences in the social field (working hours, working conditions, sanitary conditions, etc.) and impacts in the fields of food safety, environment, etc. were studied. In a second part of the research an inquiry was made at holdings that already introduced the new systems. Based on the results of the literature review and the results of the inquiry the advantages and disadvantages of the different housing systems for laying hens were formulated. The research also contributes to a better insight in corporate social responsibility. 2 1 3 Furnished cage: 1. perch 2. litter area 3. nestbox 51 2.3.1 New insights into the origin of the cultivated apple (Malus x domestica) 2.3 Plant Sciences Apple (Malus x domestica) is one of the most important fruit crops in the world and also very important for Belgium (the average annual apple production in our country is about 390,000 tonnes). Many varieties were grown in the past in Belgium by a tremendously active peasant population. However, when growers started to produce apples not only for the local market but also for export, the importance of high yielding varieties for production on large scale increased. Nowadays, as a result, apple production is dominated by a few superior cultivars, which have been derived from a limited number of parents. The question has been raised whether this limited genetic basis can jeopardise future breeding efforts in apple. If the genetic basis of the breeding programmes is too limited, inbreeding may become a serious problem making breeding progress impossible. Germplasm that can be used to increase the diversity in apple breeding programmes include both old (obsolete) varieties and wild relatives. Old regional varieties contain useful variability (e.g. with regard to disease resistance) to enrich gene pools of contemporary breeding programmes significantly. At present old apple varieties, which are no longer important in apple production but which have an indisputable cultural-historical value, are maintained in collections. Mainly two organisations focus on the conservation of old regional varieties in Belgium: CRA-W and NBS. 52 one duplication in the chloroplast matK gene in M. sieversii. It has also been suggested that M. sylvestris, a native wild apple species in Western Europe, might have contributed little or even nothing to the origin of the cultivated apple. To unravel the relationship between M. sylvestris and its domesticated relative M. x domestica, we studied chloroplast DNA variation in 634 wild and 422 domesticated accessions originating from different regions. Sixtyeight genotypes belonging to 12 other wild Malus species, including 20 M. sieversii accessions were also included in the analysis of chloroplast diversity. Marker techniques were developed to type a formerly described duplication and a newly detected polymorphism in the matK gene. Chloroplast DNA variation was further investigated using PCR-RFLP as DNA-marker technique and haplotypes were constructed based on all mutational combinations. A closer relationship than presently accepted between M. sylvestris and M. x domestica was established at the cytoplasmic level, with the detection of eight chloroplast haplotypes shared by both species. Hybridisation between M. sylvestris and M. x domestica was also apparent at the local level with sharing of rare haplotypes among local cultivars and sympatric wild trees. Indications of the use of wild Malus genotypes in the (local) cultivation process of M. domestica and cytoplasmic introgression of chloroplast haplotypes into M. sylvestris from the domesticated apple were found. Only one of the M. sieversii trees studied displayed one of the three main chloroplast haplotypes shared by M. sylvestris and M. x domestica. This is surprising as M. sieversii has formerly been described as the main maternal progenitor of the domesticated apple. This study hereby reopens the exciting discussion on the origin of M. x domestica. Also other Malus species can be used to increase the diversity of apple breeding programmes. In this case, the wild species which were involved in the origin of the cultivated apple are the best candidates. But up to now, the origin of the cultivated apple remains unclear. Currently, the most plausible theory, based mainly on morphological and molecular evidence, points to M. sieversii, a wild species from Central Asia, as the most likely maternal ancestor. This is based on the presence of large fruits and the occurrence of Malus sieversii - collection Geneva (US) 2.3.2 Valorisation of the genetic diversity of wild roses Worldwide, roses are the most important cut flower and garden plant. Although the existing variation in colours, flower forms and growth habitus is very broad, the genetic basis of the cultured rose is rather small. Studies have demonstrated that only around ten rose species are at the origin of the cultured roses. Several useful and desirable characteristics not present in modern roses can still be found in wild species. The use of this genetic diversity in rose breeding programmes is an important stimulus for the protection of these wild species in our countries. An increased habitat fragmentation and import of foreign seeds and plants constitute an important threat. The above-mentioned factors were the central idea behind the European project GENEROSE. The latter describes not only the genetic structure and the characteristics of wild roses but also the experiments to transfer the interesting traits of wild roses in modern roses. A first step in this process was the inventorising of the remaining interesting genetic resources within Europe. In Flanders the first inventory was made in 1996. This led to the discovery of some unexpected species, varieties and hybrids and a few valuable locations. In addition it was demonstrated that determination of wild rose species is complex and that correct naming of the species in situ depended on the experience and the insight of the biologist. The complex taxonomic structure of the subgenus Rosa could be confirmed based on an in-depth morphological study and two molecular techniques used viz. AFLP and microsatellites. The Flemish roses can be divided in three sections or groups: Pimpinellifolia, Caninae and Synstylae. The section Caninae is the best represented throughout Europe and can be separated in different subsections. For Flanders these subsections are: Vestitae, Rubigineae, Tomentellae and Caninae. Neither the morphological study nor the genetic analysis supports the splitting- up of the last two subsections. We therefore propose to classify also R. tomentalla under the subsection Caninae. Very typical for the rose family (Rosaceae) is the possibility to cross with each other species belonging to the same genus. In nature several hybrid forms can be found. Some of these spontaneous hybrids are considered as fixed species (i.e. R. stylosa), whereas others are sterile (i.e. R. x irregularis). The genetic techniques used and the performed controlled test crosses could not always give an univocal answer to the formulated hypotheses in relation to the origin of these hybrids. However, the supposed parental species of R. henkeri-schulzei, R. stylosa and R. x irragularis could be confirmed. Furthermore, the clonal behaviour of Rosa canina var. andegavensis R. arvensis and R. spinosissima populations was confirmed, but also the presence of different genotypes within one population seemed to be important. All the collected European genetic material was screened for resistance to the most important rose diseases (black spot, rust, powdery mildew and downy mildew). Adapted bioassays and infection protocols were developed to screen at both laboratory level and under greenhouse conditions. Finally, some promising and interesting genotypes from wild species were selected. When crosses between wild species and modern roses are made, the difference in ploidy level plays an important role. Culture roses are mostly tetraploids (2n=4x), what means that they have from each chromosome four copies. The related wild species are predominantly diploid (2n=2x) or in case of the Caninae pentaploid (2n=5x). Interspecific hybrids give therefore triploid (2n=3x) progenies, which are difficult to use in further breeding programmes. Different strategies as chromosome doubling and haploidisation are currently investigated. The results obtained so far demonstrate that both techniques can be used in roses, but their efficiency is low. The inheritance of traits from wild roses in modern cultured roses was demonstrated both morphologically and using molecular markers. 53 Heterodera schachtii the harvested crop by the distribution chain or the food processing industry. This is the case for potatoes, scorsonera, carrots, etc. Modern Flemish agricultural management uses more green manure crops, which is advantageous because of the fixation of nitrogen at the end of the season, the improvement in soil structure, the support of organic matter and prevention of soil erosion. On the other hand there is an increased risk of pests and diseases by using these catch crops. Breeding for resistance against nematodes in these green manure crops is therefore the appropriate strategy. In the green manure crops such as fodder radish and white mustard, the breeding goals are fast covering of the soil, late flowering and very importantly nematode resistance. Ten years of research at ILVO-PGV has led to a biotest for screening individual plants for their resistance against beet cyst nematodes (Heterodera schachtii). Plants are grown in a pure sand substrate under a controlled environment of temperature, humidity and light. The sand is calibrated and the fraction used has a diameter smaller than those of the cysts. About two weeks after drilling the plants are individually infected with J2-larvae of the beet cyst nematode. Resistance screening itself takes place 54 Our programme uses annual types of brassica green manure crops, thus we can screen a new (already improved) generation every year. Individual resistant plants are selected out of the most resistant populations and are the basis of new varieties. The ability of the plant to increase or to reduce the population of nematodes is given by the multiplying index: the pf/pi-score. This ratio expresses the final population (pf ) to the initial population (pi) and is a measure of the level of resistance of the plant. A pf/pi-score below 1.00 means that the final population is lower than the initial population. A score of 0.10 represents a final population that is 10% of the initial population. In case of a pf/pi-score higher than 1.00 there is an active multiplication of the nematode population. The ranking of resistance in classes by the ‘Deutsche Bundessortenamt’ is the European standard. The highest resistance class is class 1 with pf/pi 0.00 to 0.09. The recurrent family selection method practised at ILVO has resulted in a list of commercially available varieties with a high degree of resistance against the beet cyst nematode (Heterodera schachtii). Resistant cultivars of fodder radish are Maximus (class 1), Nero, Sixtus, Cassius and Sirius. Three new cultivars are actually subjected to official trials. The ILVO varieties of white mustard with high resistance are Rumba, Polka and Chacha. See table below. fodder radish The economic impact of plant parasitic nematodes in agricultural and horticultural crops is often underestimated. In Flanders the root-knot nematodes Meloidogyne chitwoodi and M. fallax on maize and carrots, the cyst nematodes Globodera pallida and G. rostochiensis on potatoes and Heterodera schachtii on beet and spinach, can cause huge yield losses. A low nematode population level retards plant growth and results in yield reduction of the crop. In the case of higher population levels, the damage affects on quality aspects, resulting in the refusal of eight to twelve weeks after drilling, by washing the roots onto a sieve and counting the number of cysts. This biotest is performed three times a year. white mustard 2.3.3 Breeding for resistance against nematodes in green manure crops (catch crops) Cultivar Pf/pi-score Maximus 0.082 Nero 0.106 Sixtus, Cassius and Sirius 0.100 - 0.200 Rumba 0.113 Polka 0.137 Chacha 0.182 With breeding for resistance against nematodes in green manure crops ILVO contributes to the demand for high performance varieties in Flemish agriculture. White mustard 2.3.4 Inventorising predatory mites in tree nurseries Pests of agricultural importance cause yearly a lot of problems for many farmers and nurseries. Also in tree nurseries, which are responsible for half of the total production value of the ornamental plant culture, there are regular problems with injurious insects and mites. In recent years, especially the phytophagous mites, including eriophyoid mites (Eriophyoidea) and spider mites (Tetranychidae), give rise to more problems in tree nurseries. The feeding injury caused by those mites depends on the species and goes from leaf discolouration to total plant deformations. These symptoms lead to unmarketable plants and result in economic losses for the nurseries. Despite the attention that has been paid to environment and biodiversity, application of broad-spectrum pesticides is still common in this sector. Frequent use of these pesticides results in environmental contamination and has to be reduced where possible. In the framework of the national reduction plan, a warning system was set up in 1997. This system enables the grower to apply insecticides at the right moment. Owing to this system of guided chemical control, the traditional calendar spraying is avoided and the numbers of sprayings are drastically reduced. To proceed in reducing pesticides biological control will be the only alternative. To control mites, releasing commercial predator mites can be an option but it’s not evident to use them outdoors. In contrast to the knowledge of biological control of pests in orchards, little is known about the occurrence of natural enemies of mites in tree nurseries. After interviewing growers and personal field observations, a suspicion of the existence of a natural equilibrium of spider mites arises in some production areas of tree nurseries. Further registration of predators and pathogens of spider mites has to lead to a better insight in this matter. A study of natural enemies of spider mites is currently done at Plant-GB. The purpose of this research is to list new and endemic tetranychids and to study the complex of their natural enemies. The emphasis goes in the first place to the main plant inhabiting predatory mites and mite pathogens. The objective is to obtain knowledge about the phenology, the occurrence and parasitism or predation capacity of these natural enemies. Besides the study of their biology, the effect of pesticides on non-target predatory mites will be observed. To establish the potential of predatory mites in tree nurseries, a preliminary study has been performed. During the growing season, tree species (viz. Fagus sylvatica, Carpinus betulus, Tilia cordata, Quercus robur, Salix alba, Acer pseudoplatanus, A. platanoides, Sorbus aucuparia and Taxus baccata) were fortnightly observed by taking leaf samples from an unsprayed field. Besides the survey, attention was also paid to the optimisation of the sampling techniques for further field observations. 55 In the laboratory, mites were extracted from the collected plant material using the Berlese funnel technique. The isolated mites, which belong to the Mesostigmata and Prostigmata, were identified in 12 families: Phytoseiidae, Ameroseiidae, Tydeidae, Cunaxidae, Bdellidae, Tarsonemidae, Tetranychidae, Eriophyidae, Anystidae, Eupodidae, Erythraeidae and Stigmaeidae. Because of the great importance of Phytoseiidae and Stigmaeidae as predatory mites of spider mites they were identified to species level using phase-contrast microscopy. All together 12,105 mites were extracted from the collected plant material. More than 60% of the identified mites belong to the family Phytoseiidae. Representatives of this family were detected in all the samples during the sampling period. Amblyseius andorsoni was the most abundant species and was found on every tree-species. The occurrence of A. andorsoni can be very important, because this predatory mite is already reared for controlling spider mites and eriophyoid mites in greenhouses. Anthoseius rhenanus and A.bakeri were found in huge numbers on F. sylvatica, this in contrast with Typhlodromus pyri, another Typhlodrominea mite. Beside the European beech, A. rhenanus was found on Carpinus betulus. Little is known 56 about the predation of Anthoseius spp. on Tetranychidae in tree nurseries. Because of the high numbers of Anthoseius spp. and the frequency with which they were found, further research has to be done to see which role they can play in biological control. Neoseiulus californicus was found on both Tilia species and Fagus sylvatica. Neoseiulus reductus was determined on Tilia cordata and, according to the literature; this is a new report for Belgium. Euseius finlandicus was only found on Quercus robur. Zetzellia mali, a stimaeid mite, was often determined on Sorbus. This mite is known as a predator of Panonychus ulmi. It feeds on the eggs and immature stages of this spider mite. Because P. ulmi is already reported in tree nurseries (e.g. on Ribes and Prunus), the occurrence of these predatory mites is advantageous. This preliminary study shows the potential of predatory mites in tree nurseries. Further research has to lead to an alternative control of phytophagous mites instead of using acaricides. 2.3.5 Infectivity under field conditions of Clavibacter michiganensis subsp. sepdonicus, causal agent of bacterial ring rot of potato. Potato ring rot is caused by the plant pathogenic bacterium Clavibacter michiganensis subsp. sepedonicus. Specific plant health requirements (Directive 2000/29/EC and Directive 93/85/EC) are imposed in the EU to protect the potato production against the introduction, establishment and spread of this organism. Reliable and relevant information on ecology and epidemiology should be available to set up appropriate quarantine constructions resulting in a sustainable protection. Control strategies implemented after a confirmed presence of the pathogen target functional eradication and may jeopardise operational farm economics. For many years, sporadic interceptions in potato imports were the only encounters with ring rot until the disease was diagnosed in the domestic potato productions of 2002 and 2003. In particular the occurrence in seed potatoes resulted in great concern when infection was confirmed in seven clonally related seed lots from which the origin after five field generations was traced back to a single source produced from mini tubers. Remarkably, not any suspicion was encountered during seven years of multiplication, neither by visual inspection nor by lab testing. Moreover, three seed lots showed levels of contamination far below 0.5% which were almost undetectable at the sampling standard applied at that time. Ring rot tubers were only found in three out of forty-seven contaminated samples which implies that latent infections were common. A major obstacle for the scientific underpinning of eradication regulations is the erratic occurrence and unpredictable nature of the disease which can slumber for many years due to latent infections. Depending on the potato variety, infected seed tubers produce more or less undetectable infection in the progeny tubers. A low transmission rate from one field generation to the next may possibly be caused by the restricted migration of the pathogen in the vascular ring of the seed tuber. The possibilities for survival outside the potato tuber are limited, unless diseased tubers contaminate materials used in potato culture such as crates, jute sacks, rubber from conveyor belts or metal from planting machines, harvesters or grading equipment. The ring rot pathogen remains remarkably persistent in a sort of bacterial paste which sticks firmly to the surface of the material and requires pressurised decontamination. Under dry and cool conditions (below 10°C) the bacterium remains viable and virulent for many months. Contamination of potato tubers through contact is therefore considered high risk for ring rot and potato lots for which contact with an infected lot has been ascertained are also removed from the trade. However, efficiency of disease transmission through contact is lacking substantial evidence. Potato ring rot During the past three years, field studies on infection and transmission of C.m. subsp. sepedonicus were performed under bio-security conditions to elucidate some of the aforementioned blind spots. Each of the experiments was secured by rows of buffer plants which also allowed us to study field transmission due to sprinkler irrigation. Differences in disease transmission via seed tubers were analysed by inoculating low levels of the pathogen on vascular tissues either at the stolon end or at the apical bud end. Infection developing from the apical bud resulted in a substantially higher disease incidence than infection developing from the stolon end: 80% of the plants with infection in the progeny tubers vs. 90% of the plants with no detectable infection in the progeny tubers. This result clearly suggests that seed tubers in which infection has not spread from the heel end into the vascular ring are likely to produce undetectable infection in the following potato generation. The risk of infection through contact contamination was analysed by pressing the cut surface of a ring rot tuber on intact seed tubers either at the stolon end or at the apical bud end. Infection of C.m. subsp. sepedonicus was demonstrated in 3.1% of the potato plants grown from seed tubers which were contaminated at the stolon end and in 12.5% of the potato plants grown from seed tubers which were contaminated at the apical bud end. During the three years of field experiments, all buffer plants remained free of infection which is considered to be 57 indicative of the absence of plant-to-plant transmission. If cutting of seed tubers was prevented, and the mother tuber was the only source of infection then a significant dilution of infection in the progeny tubers occurred with a tenfold reduction of the contamination level after two years of multiplication. A high number of samples is then required to detect these levels of infection in a seed potato lot and testing in the official programmes is now being concentrated on the superior seed potato classes. Plants We are also investigating latent infection of C.m. subsp. sepedonicus during tissue culture multiplication, production of mini tubers and following field generations. After several dose-response experiments, we succeeded in infecting potato plantlets of the varieties Agria, Désirée and Santana using very small quantities of ring rot bacteria to that extent that infection was not visually expressed during the culture period. Shoots were snapped off potato plantlets grown from tissue culture in peat substrate and were placed with the fraction edge in a diluted suspension of C.m. subsp. sepedonicus. These shoots were cultivated under a sub-optimal regime for ring rot expression to finally produce mini tubers. The pathogen was monitored serologically in the stem of the potato plants end at the heel end of the mini tubers. Latent ring rot infection was demonstrated in eighteen mini tubers which were produced by seven plants. Only two mini tubers had infection levels higher than 106. However, infected plants also produced mini tubers without detectable infection. We look forward to analyse the progeny tubers from the first field generation, in particular the productions of negatively tested mini tubers of infected potato plants. The root-knot nematode Meloidogyne chitwoodi is a major problem in the culture of field vegetables for the food canning industry in the Belgian provinces Antwerp and Limburg. Meloidogyne chitwoodi has a wide host range and has multiple generations during a crop-growing season. It causes severe damage to economically important crops such as potato, black salsify and carrot. In 1998 M. chitwoodi was listed as quarantine pest in the EU. To secure the culture of field vegetables against this pest it is important to detect a M. chitwoodi infestation as soon as possible. The multiplication of nematodes strongly depends on the host plant. As the growing season continues, the number of nematodes increases. Meloidogyne chitwoodi can develop several generations per season and therefore, even with low initial densities, can lead to severe infestations. When roots are harvested, they take away a part of the nematode population from the field. Root-knot nematodes are obligate plant parasitic and die when there is no host plant available. Roots or root pieces that remain in the soil can help nematodes to survive adverse conditions. Meloidogyne chitwoodi can build up high population levels but their number decreases markedly during winter and under black fallow. Through frequent soil sampling in infested fields, knowledge of the increase or decrease of the soil population under different field crops and black fallow is amassed. The dynamics of the nematode population are not restricted to the upper soil layers. Nematodes are mobile and can migrate in the soil under the influence of the host plant (deep rooting systems), the water table and the temperature. From these deeper soil layers nematodes can infect subsequent crops. This is important in developing an adequate soil sampling strategy. Cms infected plants bacterial ring rot plants plants with Cms infected mini tubers Dose response of bacterial stem rot infection in potato plants 58 2.3.6 Population dynamics of the root-knot nematode Meloidogyne chitwoodi under different field crops and black fallow. On two natural infested fields, the population dynamics of M. chitwoodi are monitored since spring 2004 through monthly in-depth soil samplings. Soil samples are taken up to a depth of 70 cm. Per soil layer of 10 cm nematodes are extracted with the automated zonal centrifuge technique and counted under a binocular. After summer barley and winter fallow the population of M. chitwoodi was very small in field 1 and was situated mainly in the upper 20 cm of the soil. Under the influence of carrot the population increased significantly and shifted to deeper soil layers. The highest numbers were found immediately after harvest in the soil layer from 20 to 30 cm. In the spring of 2006 a peak in the population occurred after a decrease in the winter. This can be explained by the hatching of juveniles from eggs. The highest increase of the population was recorded in the soil layers from 20 to 40 cm depth. On field 2 the population increased substantially in the deeper soil layers under beet. After the culture of beet a classical soil sampling results in a serious underestimation of the present nematode population. Figure 1A Infestation of M. chitwoodi on scorzonera Figure 1B The population of M. chitwoodi decreased strongly under bean, cv. Polder and this decline continued under marigolds, Tagetes patula cv. Single Gold. More than one and a half year after the culture of beets, the numbers of M. chitwoodi were still the highest in the soil layers from 20 to 40 cm depth. To detect 50% of the actual soil population, soil sampling up to 40 cm depth was necessary. Figure 1C Figure 1: Population dynamics of Meloidogyne chitwoodi on field 1 during 3 years. A: summer barley 2004, B: carrot 2005, C: spring 2006 59 Figure 2A Figure 2B Figure 2C Infestation of Meloidogyne chitwoodi on carot( Daucus carota) Figure 2D Figure 2: Population dynamics of Meloidogyne chitwoodi on field 2 during 3 years. A: beet 2004, B: bean 2005, C: marigolds 2005, D: spring 2006 60 The detection chances for M. chitwoodi are the highest immediately after harvest. However, the history of the field needs to be taken into account. The influence of deep rooting crops on the vertical distribution of M. chitwoodi is still perceptible one year after the crop. Based on the research on the population dynamics of M. chitwoodi under different field crops, a better soil sampling strategy can be developed and the detection changes for this quarantine organism can be enhanced. 2.3.7 Fifteen years of cooperation with the former Eastern Block countries: The democratisation process and the acquis communautaire Framework In 1990 the Belgian Government launched the so-called PHARE help programme for Poland and Hungary, later on extended to all Central and Eastern European countries, by analogy with the same named programme of the European Union and somewhat comparable with the Marshall plan after WW II between America and Western Europe. In 1992, the Flemish Government started a programme for the Cooperation of Flanders with Central and EasternEurope in all sectors where it had authority according to the regionalisation. This programme is still running. Between 1992 and 2006 Flanders spent about 85 million euros in 650 subsidised projects. From the beginning the Research Station of Plant Breeding (RvP) and later the Department of Crop Husbandry and Ecophysiology (DFE) were actively involved in these programmes (see table). Activities Up to now projects are finished or are still in implementation. In the Czech and Slovak Republic, Poland, Hungary, Latvia, Lithuania, Bulgaria, Romania and Slovenia, we stepped in on domains where, based on our experience, we could cooperate to transfer knowledge in the democratisation process and later on concerning the EU acquis communautaire. The thread through all the projects was the “improvement in yield and quality of grassland” related to variety trials, carbon storage, erosion prevention, nutrient management, etc., be it in classic or organic farming. spoken press, Flemish and foreign, was interested in our activities and many times a press article was published or a radio interview was broadcasted. In final project seminars, we always stressed and underlined the Flemish message of cooperation and transfer of knowledge to the audience and to the farmer. Manuals, courses, books and articles about the project subjects were written in English and mostly also translated in a foreign language (Bulgarian, Romanian Latvian, etc.). Conclusion The benefit of these projects is often questioned. They have nothing to do with acquirement of scientific knowledge or with a scientific research programme. It is the transfer of our knowledge to colleagues in C&E Europe for implementation in practice. After 15 years one can observe that a lot has changed in the good sense. Tens of colleagues from C&E Europe visited our institute and learned about our practice of good grassland management in all its aspects. A very important networking and cooperation was built up. The iron curtain between us and them was broken up and despite the completely different backgrounds of centralised versus decentralised economy, we understand each other much better now. It was a fascinating and fruitful experience. Now that Bulgaria and Romania will also become EU members from 1 January 2007, this chapter of cooperation will be closed as the task is fulfilled. The cooperation will be continued, but on another basis and with other financial sources. Projects were only financially supported by the Flemish Government if they were selected in Flanders and in a Central and Eastern European (C&E) country by the selection commission ad hoc. Our networking in and our knowledge of C&E Europe was also appreciated by others and our cooperation was demanded by the Faculty of Bioscience Engineering of the University of Ghent, the Belgian Farmers Union, Semper b.v.b.a., Agriv, v.z.w. We had the opportunity to meet interested ambassadors. Their moral support was well appreciated for the many days and weeks away from home. Also the written and 61 x x Flanders C&E EUR grass and forage crops Flanders C&E EUR variety trials x x Handbook “grass seed multiplication” x x x x 4 trial locations and handbook “variety trials” also in Bulgarian x x x x Flanders C&E EUR organic farming x 4 pilot farms and handbook “growing maize” Contacts with Flemish growers for the Bulgarian market x Flanders C&E EUR erosion prevention 2 trial locations and handbook “postcontrol” NIRS instrument in ABI Sofia Manual “NIRS analyses” x Flanders C&E EUR leguminous crops 8 pilot farms and handbook “Leguminous crops for cattle” 10 pilot farms in Bulgaria with erosion prevention x Furthermore projects in cooperation and coordinated by: The Faculty of Bioscience Engineering of the University of Ghent: 1) Nutrient balances in Hungary 2) Nutrient balances in Bulgaria Agriv v.z.w.: 1) UPOV membership and post-control of seeds in Lithuania 2) Pilot farm “grassland management” in Romania Semper b.v.b.a.: post-control of seed production in Croatia 62 Pilot farms and lucerne variety with decreased lignin content x x Flanders C&E EUR maize Flanders C&E EUR azaleas Networking with scientific institutes Several publications and books “grassland management” “Cultura pajiştilor” Flanders C&E EUR postcontrol Flanders C&E EUR NIRS analyses Output Slovenia Phare programma seed production x Latvia x Romania x Bulgaria Hungary Phare programme grassland improvement Slovakia Poland project Czech Rep. Projects coordinated by ex-RvP-DFE in Central and Eastern Europe Country 2 pilot farms per country and handbook “organic farming” also in Bulgarian and Romenian 2.3.8 SANCO EC- trial for grasses In 2005, ILVO Plant-TO started a trial with grasses on behalf of the EU Commission DG SANCO (Health and Consumer Protection). This project includes comparative tests and trials on seeds/plants that are delivered by the official inspection services of the EU member states. The aim of a project like this is to evaluate the inspection services on the one hand and to introduce the new member states into this kind of inspections on the other hand. The general aims of the project were formulated as followed: Where appropriate, to monitor the action taken by the Member States relating to samples that failed to meet the standards in the previous trial. To monitor whether changes in seed quality result from measures taken under official supervision under Commission Decision 98/320/EC. To develop in a harmonised way an understanding of the requirement that the seed shall have adequate identity and purity with regard to variety, together with the development of appropriate standards for the acceptance and rejection of seed lots. certified seed. Based on these tests, 18 of the delivered seed lots failed: 8 for emergence, 8 for contamination with other seed of other species and 2 for purity. In the field trial, the basic and the certified seed was sown next to the standard seed plots, this made it easier to examine possible off-types. The entire trial was set up in duplicate and every sample was sown in four rows with a length of 4 m and 35 cm between each row. Based on these field trials, 10 samples failed because there was a clear phenotypical difference between the examined sample and the standard sample. Another 11 samples gave cause for concern with regard to varietal and species purity. Parallel trials were set up in the UK and in The Netherlands, respectively by the NIAB institute located in Cambridge and the NAK institute situated in Emmeloord. On 1 July the European experts visited our trials, and our service visited the other trials in Emmeloord and Cambridge, respectively on 30 May and 8 June. The final report was delivered to the commission in October 2006. The practical content of the task was to set up tests and a field trial on seed samples of grasses that are introduced on the market under the provisions of Council Directive 66/401/EEC (dated 14 June 1966). Hereby, standard seed lots of frequently used grass varieties (for pastures and other aims) were compared with basic, pre-basic and certified seed. The standard seed is the seed that is registered and kept by the official authority as the true and original variety. The 293 samples that were sown and tested were delivered by 18 European countries. There were 14 Gramineae species tested: Agrostis capillaris, Agrostis stolonifera, Dactylis glomerata, Festuca ovina, Festuca rubra, Festuca arundinacae, Festuca pratensis, Lolium multiflorum, L. multiflorum westerwoldicum, Lolium x hybridum, Lolium perenne, Poa pratensis and Phleum pratense. Before sowing, the seed samples were analysed and tested for emergence and purity in the accredited laboratory for seed examination of the Flemish Community. For each species of grass there exists a specific norm for purity and emergence, depending on whether it is basic or Visit SANCO-trials in Merelbeke with European experts 63 2.4.1 Residual enzyme activity in UHT-milk and milk powders The refrigerated storage of raw milk creates selective growth conditions for psychrotrophic micro-organisms, which produce heat-resistant extracellular enzymes such as proteinases and lipases. Proteinases from psychrotrophic bacteria (mainly belonging to the genus Pseudomonas) can survive ultra-high temperature (UHT) processing and are associated with several technological problems during processing and storage of dairy products, such as cheese yield losses, formation of bitter off-flavours and gelling of UHT-milk. Lipases are responsible for the hydrolysis of fat, which leads to rancidity. Technology & Food This research is carried out in the framework of an ILVO PhD-scholarship in collaboration with Ghent University under supervision of Prof. K. Dewettinck (Department of Food safety and food quality). The main purpose of this project is the acquisition of knowledge and the development of methods in such a way that recommendations can be made to guarantee and improve the shelf life of UHTmilk and dairy products. In addition, methods will be developed to predict shelflife of dairy products based on the initial quality of raw milk or on the heat-treated product. 2.4 Firstly, it was investigated how the refrigerated storage of raw milk influences the development of psychrotrophic micro-organisms, which can lead to the production of heatresistant enzymes. . Subsequently, an extensive screening of the raw milk samples was carried out (both winter and summer milk). From this screening it was possible to deduce which strains of bacteria produce thermoresistant enzymes and what the relation is between the psychrotropic microbial concentration and the protease activity as function of the refrigerated storage conditions. DNA TNBS measurement 64 2.4.2 Effect of a changed milk fat composition on the quality and processability of milk typing of the proteolytic bacterial strains showed a great diversity in summer as well as in winter milk. In the future, those enzymes will be purified and (partially) characterised. Conditions for inactivation will be determined and an immunological test will be developed. Special attention will be paid to low temperature inactivation, a moderate heating process allowing the inactivation of a major part of this proteolytic activity. The interaction between indigenous (the plasmin system in milk) and bacterial proteases will also be closely evaluated. The determined criteria for heat inactivation of the enzyme will be verified in a pilot-study and by industrial testing. Finally, verification will take place for the most suited method to evaluate the quality of the raw milk and the processed products and for the prediction of the shelf life of these products. This project anticipates the position of the WHO (World Health Organisation, Genéve 2004) about the fact that food, lifestyle and physical activity play an important role as determinants of chronic diseases, such as cardiovascular diseases, cancer, diabetes, hypertension and osteoporosis. Worldwide, one-milliard adults are suffering from overweight, of whom 300 million are obese. In Belgium, about 50% of the adults have overweight of whom 10% are obese. More or less 14% of the Belgian children have a too high a weight and 5 to 10% of the children are obese. In Europe, expenses as a result of obesity are estimated at 2 to 8% of the total health costs (Obesity in Europe, 2002; Obesity in Europe, 2003; European Association for the Study of Obesity). Overweight and obesity are important risk factors for the development of chronic diseases. Key factors for these risk factors are the increased consumption of energy-rich food, rich in saturated fatty acids and sugars combined with the decreased physical activity. Animal fat, including milk fat, is rich in saturated fatty acids which contribute to an increased risk of cardiovascular diseases. In Belgium, milk fat is responsible for almost 25% of the total mean fat intake. This fat fraction is also considered to be co-responsible for the rising obesity epidemic. Moreover, it is suggested that replacing the saturated fatty acids by unsaturated fatty acids would result in a beneficial health effect. To deal with these harmful features, the milk fat composition will be adapted and improved by means of a selective feed system for milk cattle resulting in an increased contribution of unsaturated fatty acids, such as the ω-3 fatty acids and conjugated linoleic acid (CLA). Protealysis of milk isolates The research is performed within the scope of a PhD-scholarship at ILVO in cooperation with the Department of Food Safety and Quality of Ghent University under supervision of Prof. K. Dewettinck and with Dr. ir. K. Raes (Department PIH, West-Flanders). The research is a part of the chain project “From roughage to high quality milk”, running at ILVO. The Plant Science Unit is carrying out research to select grasses and clovers rich in unsaturated fatty acids by means of plant refinement programmes and research into the cultural and practical value of these sorts, respectively. The Animal Science Unit will process these selected grasses and clovers in their feed system. Research will be performed into the effect of the composition of roughage on the composition of the milk fat fraction and on the consequences for the milk production. The Technology and Food Unit is investigating to what extent the quality of the milk with a different milk fat composition differs from that 65 of standard milk. Besides, special attention is paid to shelf life and processability of the milk with changed fatty acid profile. Due to the increase in unsaturated fatty acids in milk fat, the oxidative sensitivity of milk can increase resulting in a possible development of taste defects which can shorten the shelf life of milk and dairy products. In a first phase, methods are finalised to determine detrimental oxidation products in dairy products. Based on lighting experiments, the relevance of two methods, the FRAP and DPPH method, is investigated, which measure the consumption of antioxidants in a very early stage of oxidation. Figures 1 and 2 illustrate that these methods are capable of detecting the consumption of antioxidants after 3 to 4 days of lighting. Figure 1: Determination of oxidation in pasteurised milk by means of the FRAP method Figure 2: Determination of oxidation of pasteurised milk by means of the DPPH method The DPPH method (Figure 2) is based on the reduction of the stable free radical 2.2-diphenyl-1-picrylhydrazyl by antioxidants. The antioxidative capacity is defined as the EC50-value, the amount of milk serum necessary to reduce the initial DPPH concentration with 50%. Figure 2 illustrates that a higher amount of milk serum is necessary to reduce 50% of DPPH as the lighting time increases. Also the physical features of milk fat, and especially the crystallisation pattern can be changed for milk with a changed fatty acid profile, which can influence the processability of this type of milk. A second phase of the research will focus on the extent to which process conditions and possibly the composition of the dairy products need to be adapted in order that dairy products are obtained with comparable physico-chemical features as the ones with an unchanged fatty acid profile. The FRAP method (Figure 1) is based on a redox reaction in which an excess of Fe3+ is reduced to Fe2+ in the presence of antioxidants. Figure 1 illustrates that the capacity of the milk to reduce Fe3+ decreases during storage. 66 Lighting experiments with pasteurised milk 2.4.3 Mechanisation: A good application technique for more efficient entomopathogenic nematodes Entomopathogenic nematodes (EPNs) are deadly pathogens for insects. These nematodes contribute to the regulation of the natural population of insects, but they can also be used as biological insecticides. The advantages of the use of EPNs in comparison with the use of classical synthetic insecticides are not so obvious when only efficacy and labour costs are considered. Concerning the impact on the environment and human health EPNs offer numerous advantages in comparison with synthetic pesticides. Their efficacy is very specific and as a consequence they are harmless to beneficial insects. The user can apply them without any health risk, they are safe for the environment and their use can contribute to lower pesticide residues in food. Moreover, they can offer solutions for specific crop protection problems in organic farming. These advantages make investments in research on optimising the efficacy of these products worthwhile! Contrary to synthetic pesticides, EPNs are living organisms. This poses a great challenge on the level of formulation and application technology, as the nematodes need to survive the application process. For the moment, only few research-based guidelines exist on the application of EPNs while these guidelines are necessary for the acceptance of these products by farmers. the effects of pump type, mixing system, nozzle type and size, and filter size on the survival and deposition of the nematodes (Figure 1). The set-up will also contain an injection system for evaluating the efficiency of this system to inject the nematodes intact in the spray solution. The goal of the experiments is to obtain general guidelines for the application of EPNs. Nematodes The use of different spray application techniques for the application of EPNs is still under discussion. A first group of scientists is proponent of the adoption of existing techniques; the second group believes it is necessary to develop new techniques for the application of EPNs. From a commercial point of view it is obvious that there is a need for products that can be applied with existing techniques, because it is very unlikely that farmers will invest in new application machinery. Anyhow, even if new systems will be developed, firstly and especially there is a need to investigate the restrictions of the existing application techniques. At the Institute for Agricultural and Fisheries Research, a PhD-research is currently undertaken on the optimisation of the application technique of EPNs. A modular spraying system is being developed and will be used to evaluate 67 Figure 1: Modular spraying system Figure 2: Analysis of a sample using image processing Usually, the analysis of nematode samples is carried out microscopically with the use of a count dish. This is however a method that demands a lot of experience and time. To make the analysis of the samples (cfr. experiments with modular spraying system) less labour intensive, an image processing technique is under development (Figure 2). With a digital camera, pictures are taken from the sample and analysed using the Halcon and/or ImageJ software. By means of time lapse photography the difference is made between living and dead nematodes. Besides the effects of the application technique of EPNs, the survival, deposition and distribution of the nematodes on the target (soil or plant) will be investigated. EPNs can only move over a short distance, that is why the application technique needs to bring the EPNs as close as possible to the target organisms. The experimental set-up to evaluate this parameter will be developed next year. At a later stage of the project attention will be paid to the effect of different adjuvants on the survival of EPNs. Especially under field conditions, EPNs suffer from dehydration and UV-damage. Moreover, the droplets of the spray solution deposited on leafs (with foliar applications) are, without any adjuvants in the solution, very sensitive to runoff. On the other hand nematodes can also be captured in droplets that remain on the leafs. Without the addition of surfactants, separate droplets only cover a small part of the leaf surface, and there is only a small chance that the nematodes will meet the target insect. The ultimate goal of this research is to optimise the application of EPNs. Experiments performed with the modular spraying system must enable the development of a prototype spraying machine for the application of these organisms. This prototype will be tested in combination with the selected adjuvants (2nd part of the research) under semi-field and field conditions. Analysis of nematode samples by means of a stereomicroscope 68 2.4.4 Livestock engineering and constructions - Automatic detection of lameness in dairy cattle Monitoring cattle health and behaviour is an essential but time-consuming activity for the dairy farmer. The early detection of cattle diseases or problems has a large influence on management and farmer income as well as on animal welfare. The farmer needs to know whether and when a cow is on heat, suffers from mastitis or another disease and whether she is lame or not. Atypical behaviour may be an indicator for these situations. Observation of these problems by the farmer requires a lot of effort, time and training. Acceptable detection level is often not within reach in normal practice, and very often monitoring technology is used such as e.g. step counters to detect oestrus. Although commercial applications are becoming available, the automatic detection of lameness is still in full development. Lameness may be defined as an abnormal gait (reduced speed and ground contact force of the cow, a back arch curving and a lowering of the head) in an attempt to minimise pain. This pain is often a consequence of claw and feet damage or inflammation. This abnormal gait, unfortunately, is very often only noticeable with acute illness or damage or when the inflammation is in an advanced phase. Only a trained observer may notice the onset of lameness by multiple subtle gait aberrations (for a given cow). Other claw or feet diseases may even not result in an abnormal gait pattern at all. Finally, a healthy cow might be walking “funny” because of a full udder or some stiffness after lying down. It will be an even greater challenge to choose and develop the tools that will facilitate this discrimination between healthy and pre-clinically lame cows automatically. These tools should be able to measure relevant gait-specific parameters in a cost-effective yet repeatable way. A first objective of this research is to determine the effects of claw and leg problems on lame behaviour. This lameness will be assessed both while walking or when the cow stands (loading of the claws). Experiments are done in cooperation with Ghent University, Faculty of Veterinary Sciences. These experiments are done mainly during winter and a few months after calving when claw problems are very common. Figure 1: Experimental barn with walking corridor and camera (upper left), box with pressure mat (middle left) and hoof treatment-box for claw scoring (bottom left). The return alley or waiting/feeding place is at the right. Figure 2 represents a typical pressure distribution record on the medial and lateral claw of both hind feet. Figure 2 shows the pressure mat that shows high (red) and low (blue) pressure zones at the left and right hind feet ground contact zone during a static measurement. Figure 1 shows an overview of the experimental set-up where consecutively, the gait pattern, the pressure distribution beneath the hind feet and overall claw health are being assessed. This is done weekly during the winter cowhousing period for some 20 cows. 69 Distance (m) Analysing the (filmed) gait pattern represented in Figure 3 gives information on cow speed, step length and duration, step overlap, duty cycle etc. Time (S) Figure 3: The longitudinal feet contact coordinates (in walking direction) vs. time measured with a camera on one single walking cow. While walking, the cow is supported by two and three legs alternately. For now, these results are processed manually with our first research objective in mind. If claw and leg health is indeed detectable by measuring gait changes, then a more practical (automatic) measurement and classification technique will be needed to use this as a claw (or cow) health management tool. Figure 4: Concepts for a method to detect lameness automatically: Force or pressure plates: (top left), Vision systems (bottom left). Useful variables (middle) and gait analysis classification systems (right). 70 A second part of the project focuses on the development of an automatic measurement set-up. This is being done in cooperation with the Faculty of Bioscience Engineering at the KULeuven. The concepts that are considered are presented schematically in Figure 4. Only measurement techniques that do not require any sensor or marker to be attached to the cows’ body are considered. While cow feet make ground contact, position and/or force may be measured with respect to time. This is usually performed with position and/or pressure mat systems or force plate measuring devices (high quality weighing balances). With current technology, choices need to be made because there is always a trade-off between measurement range (e.g. total surface area), resolution and accuracy (considering most available and reasonably affordable systems). Some devices appear useful in measuring multiple footsteps in one cycle, whereas other devices provide detailed information of one single footstep. Together, all these systems yield additional information about the overall gait pattern: • • • • Pressure mat (Rsscan): Very useful to measure relative pressure distribution on a restricted area. A pressure mat of 1 m by 0.3 m is available for our research. Force mat (Emfit): A rather affordable alternative to measure the total force applied on a rather large area (3 m by 0.6 m). Position mat (Gaitrite): Very useful to measure positions on a large area (4.9 m by 0.6 m) with 15 mm resolution. A very coarse and relative pressure scaling is also available. The purchase of this device is considered to measure gait without a camera in places where a vision system is (yet) impossible. Force plate (Kistler): A very useful tool to measure all components of the ground reaction force very precisely while a foot strikes the (rather limited) surface of the plate. A force plate of significant size is very heavy and expensive. The measurement of kinematic variables such as head, back arch and feet movement may be done remotely. For now, a vision system has been chosen and the development of this system is a task for the Faculty of Bioscience Engineering at the KULeuven. 2.4.5 Protection and safety engineering - Ammonia emissions from naturally ventilated buildings The acid rain phenomenon disturbs the ecosystem (soil, air, water) seriously. Traffic and industry on the one hand are responsible for NOx and SO2 emissions; agriculture on the other hand also contributes to the pollution through ammonia emission. In Flanders, 95% of the ammonia in agriculture, originates from animal breeding, 40% thereof comes from cattle breeding. Besides damage to the environment, there are also negative effects on the health of people and animals as a result of the excessive ammonia levels inside livestock buildings. In a European context the ammonia emissions in Flanders have to be reduced with 40% by 2010. Low ammonia emission techniques for the application of slurry on land should reduce the ammonia emission with 25%. An additional reduction of 15% can be reached through suitable building techniques, feeding strategies and other measures. Therefore, the Flemish government introduced a “List of Building Techniques for the Reduction of Ammonia Emissions”. This is a dynamic list, so the existing systems will be evaluated and new techniques can be added to the list. However, at the moment there is only a measuring procedure for mechanically ventilated buildings. Low emission building techniques with natural ventilation are not accepted to the “List of Building Techniques” and cannot be applied. Therefore, we examined in this project the possibilities to develop a practical and payable measuring method. This research was done in cooperation with KULeuven that developed a measurement tool for ventilation rate in naturally ventilated buildings. T&V-AT carries out the ammonia concentration measurements. Ammonia concentration measurements were performed in the experimental cubicle house of Agrivet (Ghent University). The main objective of these measurements was to investigate the ammonia concentration gradient in an animal house. Especially the ammonia concentration gradient in the vicinity of air in- and outlets was measured. A good understanding of the ammonia concentration gradients can help to determine the ideal sampling points. The results showed that the outside wind speed had the greatest influence on the ammonia concentration at a specific point in the cubicle house, the wind direction was the second most influential parameter. The other climate parameters (radiation, relative humidity, precipitation and temperature) had no significant influence on the ammonia concentration. The wind direction determined to a large extent the measuring points in the cubicle house where the highest or the lowest ammonia concentration was measured. The (absolute) ammonia concentration gradient became smaller with increasing wind speed. It should be noticed that with higher wind speeds, little differences in ammonia concentration cause immediately (large) diffe- rences in ammonia emission because of the higher ventilation rates. During one month ammonia concentrations were recorded at eight different places (at cubicle frame height) throughout the cubicle house. The maximum difference in mean concentration between two measuring points was 35%. For one specific wind direction the difference increased to more than 100%. A large ammonia concentration gradient was found in the vicinity of the space-boarding. Remarkable is that both leeward and windward the ammonia concentration increased with increasing distance from the space-boarding. However, the equilibrium state of ammonia concentration was reached earlier leeward (usually at 70 cm from the space-boarding) than windward (130 cm). Another conclusion was that with lower wind speeds the equilibrium state was reached earlier. However, the absolute concentration gradient was higher compared with the gradient measured with high wind speeds. Besides ammonia concentration measurements observations on defecating and urinating behaviour were done in the same cubicle house. As generally known, the release of ammonia from slurry is the result of the conversion of nitrogenous compounds present in animal excreta. The major nitrogenous compound in cattle excreta is urea. The conversion from urea to ammonia is catalysed by the enzyme urease. Micro-organisms that are abundantly present in faeces produce urease. So, the defecating and urinating behaviour of cows affects ammonia volatilisation in cow houses. The animal house was divided into several imaginary zones to perform the observations. The moment and the number of times that cows defecated or urinated were recorded. The results show that there are zones where the cows defecate or urinate more. The following factors appear important: the location of the milking robot, feeding automat, feeding fence, water trough, the travelling path of the cows, etc. There was also a variation in time 71 and the moment of feeding for example influenced this time-dependent behaviour. The influence of place and time-dependent defecating and urinating behaviour on the ammonia concentration at a particular location in the stable could not – at least not through this experiment – be examined because of the too great an influence of the outside wind speed. Nevertheless, considering the general patterns (emission surface and amount of urea) regarding ammonia emission the defecating and urinating behaviour must have an influence on the ammonia concentration (gradient) in the cubicle house. In addition to the measurements in the cubicle house T&VAT developed and constructed also a set-up for ammonia emission measurements. The latter (situated in a shed on the site of T&V-AT) consists of three small slurry pit compartments for simulating the ammonia emission from a slurry pit and/or a slatted floor surface under different conditions. Each compartment contains a slurry pit (width = 1.10 m; length = 2.80 m, height = 1.25 m), a slatted 72 floor and a wind tunnel. With fans at one side of the wind tunnels the airflow can be continuously controlled and registered. The set-up can be used to examine the relation between ammonia emission and the following parameters: wind speed over the slatted floors, slurry level in the pit, manure composition and type of slatted floors. 2.4.6 PCBs in eggs originating from different production systems From these results, we conclude that there are no problems with the concentrations of PCBs in eggs in relation to the Belgian norm. 45 40 35 30 25 20 15 10 µg/kg vet (som van de 7 merker congeneren) At ILVO-T&V eggs were gathered originating from different European countries and from different production systems, namely organic eggs, cage eggs, barn eggs, free range eggs and eggs obtained from private breeders. From these eggs a number of samples were selected ad random for the analysis on the presence of PCBs (number per production system is indicated in Figure 1). This analysis was based on the Beltest I14 method where after extraction of fat from the eggs, a given amount of fat was cleaned up on columns filled with sodium sulphate, acidified silica and deactivated aluminium oxide after which the final detection of the seven marker congeners was done by gas chromatography – mass spectrometry. For the organic eggs, cage eggs, barn eggs and free range eggs similar results were obtained with maximum values between 27 and 42 µg/kg fat and average values between 11 and 16 µg/kg fat for the sum of the seven congeners. For the eggs obtained from private breeders, a maximum value of 106 µg/kg fat and an average value of 34 µg/kg fat for the sum of the congeners was obtained. The ANOVA analysis performed on the results obtained showed that there is no statistical significant difference between organic eggs, cage eggs, barn eggs and free range eggs, but there was a significant difference between eggs from these production systems and eggs obtained from private breeders where the values obtained were roughly higher (Figure 1). This can probably be explained by the contamination of the environment (accumulation of difficult biodegradable contaminants in the soil to which hens kept outside are exposed). The exposure of the laying hens to the outside environment is more restricted in professional farms than in private breeding. Moreover, the feed for the hens used by private breeders is less checked. The results of this study confirm the results obtained in a study conducted by CODA-CERVA at Tervuren. µg/kg fat (sum of 7 marker congeners) PCBs (polychlorinated biphenyls) are chemical substances that are used on a large scale since 1930 in products such as insulation or cooling liquids, synthetic materials and polishes. But because they are very soluble in fat and hardly biodegradable they are present in the environment and in the food chain. In 1991, the European Union issued a Directive banning the further commercialisation of these substances, but due to the permitted transition period it will take of course time before the existing PCBs are out of use. As a result of the dioxin and PCB crisis in our country, maximum values were set in 1999 for animal feed and human food substances and control programs are performed on animal feed, eggs, milk, etc. For eggs the Belgian norm is 200 µg/kg fat for the sum of the seven marker congeners. 5 0 bio (n=(n=33) 33) s c harrel (n= 16) private (n=39) partic ulier (n= 39) organic bam (n=16) k ooi (n= 37) vrije uitloop (n= 20) cage (n=37) free range (n=20) ty pe ei type of egg Figure 1: ANOVA analysis of the obtained PCB concentrations in eggs originating from different production systems (95% confidence interval) 73 2.4.7 Residue formation of sulphonamides in honey by migration from contaminated beeswax In the framework of the quality control of Flemish honey, residues of sulphonamides were found in 12 out of 203 tested honey samples. In Europe no maximum residue limit (MRL) for sulphonamides in honey is fixed. The contamination concerned in some cases high concentrations of sulphamethazine (up to 13,000 µg/kg) caused by illegal use of sulphonamides by the beekeeper to prevent or treat nosemosis. In four cases however it concerned a slight contamination of the honey with concentrations of sulphonamides beneath 50 µg/kg. The producers involved all claimed not having used antibiotic substances. When performing residue control on beeswax intended for the production of wax foundations, sulphonamide residues were found in imported wax as well as in beeswax from treated hives. A migration test in collaboration with the Laboratory for Zoophysiology (Prof. F. Jacobs, Ghent University) was set up to study this problem. Beeswax, free from sulphonamides, was spiked with sulphamethazine in three different concentrations. Wax foundations were prepared from this spiked beeswax. A small amount of the wax foundations was sampled for sulphonamide residue analysis by liquid chromatography-tandem mass spectrometry. After framing, the three wax foundations were placed into different hives for building out honeycombs. After bees built the combs the latter were placed in a honey supper during full summer blossoming. Once the cells were filled with honey and sealed by the bees, the combs were removed and further incubated in an incubator at 35°C. At regular intervals the honey was sampled for sulphonamide residue analysis. 74 The results of this study showed that sulphonamides are able to migrate from contaminated beeswax to the honey kept in the contaminated combs. The higher the concentration of the sulphonamides in the wax, the more residues were present in the honey. The carry-over from sulphamethazine from beeswax to honey was about 1%. The concentration of sulphamethazine in the honey was steady during three consecutive samplings with intervals of 1 month. This study showed that the purchase and use of contaminated wax foundations by the beekeeper can lead to measurable contaminations of the honey, which could be a possible explanation for the weak contamination of some honey samples found in 2003. 2.4.8 Bacteriological contamination and infection of shell eggs in the production chain Bacteriological contamination of eggs can have important consequences. Concerning shell eggs, contamination of the egg content can cause rot or food related infections. Eggs are one of the major sources for human salmonellosis. There are two important routes for contaminating the egg content: either vertically or horizontally. Vertical or transovarian transmission occurs when the egg contents are infected during their formation in the hen’s ovaries. Horizontal transmission occurs when freshly laid eggs are subsequently exposed to an environment contaminated with micro-organisms which penetrate the eggshell. The Storing shell eggs, whether temporarily refrigerated or not, resulted in a significant decrease in bacterial eggshell contamination. Eggs collected from non-cage housings were initially more contaminated on the shell compared to cage eggs. UV irradiation seems to be a simple and efficient method to reduce the general bacterial eggshell contamination. In the second stage of the research project bacterial eggshell penetration and egg content contamination were studied. Of the investigated eggshell characteristics, the cuticle deposition seemed to be the only characteristic influencing eggshell penetration. The degree of bacterial contamination of the eggshell influenced not only the eggshell penetration but also the contamination of the egg content. In the study, eggshell penetration and egg content contamination were also correlated with the identity of different bacterial species. The findings of this research supported the hypothesis that the frequently occurring Salmonella Enteritidis contamination of the egg content is mainly due to the vertical transmission route. Finally, the presence of condensation on the eggshell influences eggshell penetration significantly but has a smaller impact on egg content contamination. Candling of the bacterial penetration of the eggshell bacterial load, the bacterial identity, the eggshell quality and extrinsic circumstances are some factors affecting the horizontal transmission. In the research performed at ILVO-T&V some aspects of the above-mentioned factors were studied. This research was subject of a PhD-study (Koen De Reu, 2006) carried out in cooperation with the Department of Food Safety and Food Quality (Ghent University) and promoted by Prof. M. Uyttendaele. In the first stage of the research project the global bacterial eggshell contamination was estimated. The contamination progress in the egg handling chain, the influence of storing shell eggs and the influence of the housing system for laying hens on the bacterial eggshell contamination were studied. In the chain of collecting, packaging and distribution of eggs, few critical points for eggshell contamination were found. Nestbox in a housing system with furnished cages 75 2.4.9 Identification and harmful effects of the aerobic spore microbiota in raw milk Aerobic sporeformers belong to the genus Bacillus and allied genera. These micro-organisms produce spores which survive heating processes such as pasteurisation used in the dairy sector. Spores of some species even survive more severe heating processes such as ultra-high temperature (UHT) processing, which should give commercially sterile dairy products. When spores germinate and grow in heattreated dairy products such as consumer milk, enzymes can be produced which are detrimental to the quality of the product. This spoiling potential is strongly species-dependent. Furthermore, a limited number of sporeforming species such as Bacillus cereus can be pathogenic. As raw milk is a source of sporeformers, it is important to know the identity and the possible hazardous effects of these micro-organisms. This allows to set up “early warning” systems in the dairy sector and can lead via directed measures to the guarantee or even improvement of the quality of the raw milk. In a concluded IWT-agricultural project in collaboration with the Laboratory of Microbiology of Ghent University (Prof. P. De Vos), the representative aerobic spore microbiota was isolated from raw milk samples (periods late summer/autumn and winter) from 5 organic and 5 conventional dairy farms after a heat treatment of 10 min at 80°C. The isolates were picked up on the basis of colony morphology and purified. A first identification of the isolates was achieved through gas chromatographic cellular fatty acid analysis (FAME) via the MIDI-identification system. Further on, some representatives of the different FAME-clusters were identified via 16S rDNA sequencing. The harmful enzymatic properties (proteolysis, lipolysis, phospholipolysis and lactose fermentation) of the isolates were analysed with differential media and quantitative enzymatic tests. Finally, the possible toxin production of the isolates was evaluated with cytotoxicity tests and PCRbased tests. Ultimately, 824 strains were isolated from raw milk. Bacillus licheniformis, B. pumilus, Ureibacillus thermosphaericus, B. clausii, B. amyloliquefaciens, B. subtilis, Spores of Bacillus ruris sp. nov. 76 B. circulans and isolates of the B. cereus group were the most abundant in raw milk. The largest diversity in species was noted in milk from conventional farms. It was demonstrated that 5% of all isolates belonged to currently undescribed new species. The species Ureibacillus thermosphaericus and U. suwonensis seemed almost exclusively to be present in milk from conventional milking farms, while isolates of the B. cereus group were predominantly from milk of organic farms and almost exclusively from the summer period. A possible explanation for this species distribution could be the different feeding strategies of conventional and organic milking farms. From an analysis of the enzymatic properties on the differential media, predominantly the following species showed proteolytic, lipolytic and/or phospholipolytic activity: Paenibacillus spp., B. licheniformis, B. subtilis, B. amyloliquefaciens, B. pumilus, B. clausii, B. circulans and the B. cereus group. From the quantitative enzymatic tests it followed that B. subtilis, B. cereus and P. polymyxa were strong proteolytic organisms, and that some strains of B. subtilis and B. pumilus showed strong lipolytic activity. B. licheniformis, B. clausii and P. polymyxa were able to ferment lactose with gas production. Enterotoxins were detected for B. pumilus, B. amyloliquefaciens, B. subtilis, B. sphaericus and B. cereus. Now that the composition of the most important harmful aerobic sporeformers in raw milk is clear, a detailed study on the factors contributing to the carry-over of these organisms can be initiated. 2.4.10 Reduction of Salmonella shedding by mid-chain fatty acids and botanic compounds in a in vitro simulation of the pig’s cecum Salmonella is a micro-organism that causes many cases of human salmonellosis each year and that can be transferred from animals to humans. As the recent vaccination of laying hens seems to provoke a drastic drop in the number of Salmonella Enteritidis cases caused by the consumption of eggs, it now seems that pork comes more in the picture as possible cause of salmonellosis. As a result, European legislation imposes in the near future testing and certification of the Salmonella status for the community trade of pigs. Pigs can be Salmonella shedders as well as carriers. The reduction in the shedding of Salmonella in pigs could already constitute an important measure to break the contamination cycles on pig farms and hence to lower the infection pressure in the pig production chain. In a PhD-thesis (Nadine Botteldoorn, 2006) performed in collaboration with the Department of Bacteriology and Poultry Diseases of Ghent University with Prof. F. Pasmans as promotor, it was indeed shown that the supply of Salmonella positive pigs to the slaughterhouse is one of the most important contamination sources for the carcasses besides cross contamination from the slaughterhouse environment. A comprehensive typing study of the isolated Salmonella strains showed a strong dependence of the importance of both contamination sources on slaughterhouse management. A further fundamental insight was also accomplished via a real-time RT-PCR study of the expression of a specific gene (mntH) in the survival capacity of Salmonella Typhimurium in the macrophage of the pig; this feature plays an important role in the persistence of Salmonella in the pig and hence in the development of carrier animals. In an IWT-agriculture project in collaboration with Prof. S. De Smet (Department of Animal Production, Ghent University), the effect of mid-chain fatty acids (MCFA) and of botanic compounds was evaluated on the shedding of Salmonella in an in vitro simulation model of the microbiota of the pig’s cecum. MCFA, like caprylic acid, caproic acid and capric acid are promising additives to modulate the gut flora in a manner benefiting gut health as well as zootechnical performances. Furthermore, much attention is recently given to the possible antimicrobial activities of secondary metabolites of plants such as carvacrol, thymol, eugenol and cinnamaldehyde. The in vitro simulation model of the pig’s cecum consists of an anaerobic fermentation system which is inoculated with the cecum content of different slaughter pigs and which is fed at a constant rate with a medium containing cellulose and mucin. The advantage of this system is that many compounds and/or conditions can be screened rapidly without test animals and that animal-to-animal variation is limited because of the controlled fermentation conditions. The cecum was chosen because it is a multiplication site of many bacterial groups amongst which Salmonella so that a reduction in this part of the gut must have an effect on the excretion in the faeces. In the in vitro system an inoculated Salmonella Typhimurium strain could maintain itself at a population density of 5 log units per ml. The effect of MCFAs and some botanicals on this Salmonella strain and on other bacterial groups was followed by means of plating on selective media and via molecular fingerprinting with denaturing gradient gel electrophoresis of the bacterial 16S rDNA. The MCFA effect seemed to be dependent on the chain length and the concentration of the acid. At a dose of 15 mM, corresponding with an economic feasible concentration in pig feed, a clear inhibiting effect on Salmonella and coliforms was noticed with caprylic acid, while the other bacterial groups (lactobacilli, total aerobic and anaerobic count) were not influenced. Of the botanicals, also cinnamaldehyde seemed to have a clear Salmonella inhibiting effect. Caprylic acid and cinnamaldehyde could thus be potential Salmonella inhibiting additives in encapsulated form for pig feed. In vitro simulation model of the pig’s cecum medium waste 77 3.1 Comparison of hygiene status and animal welfare in furnished cages and non-cage systems for laying hens Traditional battery cages for laying hens will be prohibited in the European Union from 2012, following EU-directive 1999/74. From 2012 onwards, only furnished cages and non-cage systems (floor housing and aviaries) will be allowed. In Belgium, the EU-directive was converted into national legislation in October 2005. In this conversion, the European rules were followed, but space was left for adaptation of the national legislation in 2010. Before the decision whether or not to adapt the legislation will be taken, the results from two studies will be analysed: a socioeconomic study and a study on hygiene status and animal welfare of laying hens in furnished cages and non-cage systems. Both studies were conducted by the Institute for Agricultural and Fisheries Research (ILVO) in a co-operation between the Animal Science Unit, the Technology and Food Unit, and the Social Sciences Unit, together with the Provincial Centre for Applied Poultry Research in Geel, Belgium. 3. Extra highlighted Within the study on hygiene status and animal welfare, an international workshop was organised, in which a method was developed to evaluate welfare of laying hens on-farm. This method was used to compare welfare and hygiene status of laying hens in furnished cages (FC) and non-cage systems (NCS). At the Centre for Applied Poultry Research, the effectiveness of cleaning and disinfection in furnished cages and in an aviary system was compared. Furthermore, the presence of probiotic bacteria in the intestine and the vagina and a number of indicators of stress and immunity were studied in laying hens housed in both systems. 78 In the on-farm comparison of hygienic status and animal welfare, NCS performed better than FC regarding behavioural possibilities. Birds in NCS showed more foraging and walking and perched more during the day than birds in FC, the latter spending more time standing and sitting. Birds in NCS were also less fearful than birds in FC. Furthermore, the wing and keel bones of birds in NCS were stronger than those of birds from FC. Mortality was lower in FC than in NCS. Also, the incidence of keel bone fractures was lower in FC and fractures were less severe than in NCS. Furthermore, the dust concentration in the air was lower in FC compared with NCS. The FC also performed better regarding the number of aerobic bacteria in the air and on the eggshell. The number of Enterobacteriaceae in the air and on the eggshell was not significantly different between both systems. When all welfare indicators were combined in an overall welfare score, NCS scored better regarding welfare than FC. However, both systems clearly have their pros and cons regarding welfare. In the study at the Centre for Applied Poultry Research more aerobic bacteria were found on different equipment surfaces and in the air of the aviary systems compared with the FC at the end of the laying period. Still, it proved possible to reduce the number of bacteria by cleaning and, mainly, by disinfection to the same level as in FC. No A non-cage system (floor housing) systematic difference was found in surface contamination with Enterobacteriaceae between both systems. In the study at the Provincial Centre there were no systematic differences between systems in the presence of probiotic bacteria in the intestine or the vagina. Furthermore, no differences were found in the indicators of stress and immunity between the furnished cages and the aviary system. Based on the results of this study, we can conclude that both FC and NCS have pros and cons regarding hygienic status and animal welfare. NCS scored better than FC in the overall welfare score. However, the higher mortality rate, the higher percentage of birds with keel bone fractures and the higher levels of dust and bacteria compared with FC are cause for concern. In the FC, it seems important to provide the birds with enough space and behavioural possibilities. From the study at the Centre for Applied Poultry Research we found that, although the aviary system was more contaminated with germs than the furnished cages, cleaning and disinfection was equally effective in both systems. At the Provincial Centre there were no differences in probiotic bacteria in the intestine and the vagina and no differences in indicators of stress and immunity between the systems. Furnished cages for groups of 40 birds 3.2 Dietary ω-3 fatty acids: effect on performances and incorporation in milk and meat using the rabbit as model lations. Changes in fatty acid profile are partly speciesdependent. However, in a minor number of experiments it has been shown that a diet rich in ω-3 fatty acids favours the performances or animal health (survival, production duration, etc.). The objective of our research was mainly to verify this hypothesis using an animal species with a very fast reproduction cycle. In one year it is possible to obtain 7-8 litters with rabbits. To increase dietary ω-3 fatty acids, extruded linseed known for its high content of linolenic acid (C18:3ω -3) was used. The performances (fertility, prolificacy, milk yield, kit’s weaning weight, etc.) of females fed the ω-3 rich diet were not significantly better than those fed the iso-caloric ω-6 diet. However, per initial female, 10% more kits were weaned during the experiment as a result of the higher number of females that reached the end of the trial. Before weaning, kit mortality was comparable between diets, however after weaning a significant lower mortality was observed in rabbits fed always with the ω-3 diet. The dietary ratio of ω-6/ω-3 fatty acids was clearly reflected in the milk. The diet with 12% extruded linseed tripled the linolenic acid content in leg meat and leads to a ratio ω-6/ω-3 of only 1.3. Switching from one diet to the other two weeks before slaughtering leads to a strong incorporation, respectively reduction in ω-3 fatty acids. The Department of Health recommends a daily intake of 2 grams of linolenic acid. When extrapolating this to our research, then 12.5% of our daily requirement is fulfilled with the consumption of a conventional rabbit leg (weight: 200 grams). The same leg, originating from rabbits fed the ω-3 diet covers approximately 35% of our daily requirement. Consumers’ interest in health- promoting aspects of food is increasing. Fat and its composition play an important role in functional food. In contrast with saturated fats, unsaturated fatty acids and especially those which cannot be synthesised by the body are considered as very valuable in a balanced diet. Sufficient ω-3 fatty acids at the expense of ω-6 fatty acids are recommended in a healthy human diet leading to an increased protection against heart and cardiovascular diseases, against certain cancers and to an improved immunity status and are even linked to overcome depressions. In many experiments with various animal species, it is shown that the fatty acid composition of milk, meat and eggs can largely be modified through nutritional manipu- Meat pieces of a rabbit 79 3.3 2006: A productive publication year for ILVO-Fisheries During the past year, six books were published with contributions of several scientists of ILVO-Fisheries. Reason enough to draw some extra attention to these scientific publications. • Phillips B. (Ed.) (2006) Lobsters - Biology, Management, Aquaculture and Fisheries. Blackwell Publishing, 528 p. Lobsters are one of the most important commercial groups of animals harvested and farmed throughout the world. Extended over 15 chapters, a detailed overview is given about these fascinating creatures. Growth, reproduction, larval development, ecology, behaviour, phylogeny, pathology and nutritional value of both cultured and wild lobsters are covered exhaustively. The second part of the book concentrates on the six most important genera, namely Homarus, Jasus, Panulirus, Palinurus, Nephrops and Scyllarides, with a detailed description of the parameters mentioned above for most species within each of these genera. The lobster book is intended as the unique source of reference for fisheries biologists, fish farmers, marine biologists and ecologists. The book was edited by Bruce Phillips (CUT, AUS), and written by an international team of 38 authors. Together with Mike Bell (CEFAS, UK) and Ian Tuck (FRS, UK), Frank Redant (ILVO-Fisheries, Section Fisheries Biology & Aquaculture) wrote chapter 13 ‘Nephrops species’, on the presence, population dynamics, fisheries and management of langoustines in the world. The book counts 528 pages, contains 178 illustrations and costs 125 £. ISBN: 978-1-405-12657-1 and ISBN10: 1-405-12657-4. More information can be found on www.blackwellpublishing.com. 80 • Motos L. & Wilson D.C. (Eds.) (2006) The Knowledge Base for Fisheries Management. Developments in Aquaculture and Fisheries Science, Vol. 36. Elsevier, 476 p. Fisheries are in a state of crisis throughout the world. While there has been some success, truly effective fisheries management seems beyond our grasp. The knowledge needed for a proper management contains a broad array of facts and connections, from statistical assessments of the fish stocks to the information that allows government agencies to track compliance with rules. This book is quite exceptional as a scientific publication regarding fisheries science as fisheries management is dealt with from different, multidisciplinary perspectives. Leading researchers in fisheries, specialised in biology, ecology, economics, sociology, anthropology and politics, examined how management institutions can learn from their past management, but also how this information can be employed to design a more efficient fisheries management. The seventeen chapters represent a picture of the many issues involved in creating, collating and communicating the knowledge needed for a sound management. Besides an introduction and a summary, the book is divided in 3 sections, covering a wide range of political regi- mes and professional management systems, both in and outside Europe. The book is the main product of the initial phase of the EFIMAS project, a collaboration between 29 European research institutions. It was edited by Lorenzo Motos (AZTI, ESP) and Douglas Wilson (IFM, DK) and written by an international team of 27 experts. Wim Demaré (ILVO-Fisheries, Section Fisheries Biology & Aquaculture) wrote chapter 11 on ‘Fisheries management and advice in Europe’. The book counts 476 pages and costs 145 €. ISBN-13: 978-0-444-52850-6 and ISBN-10: 0-444-52850-4. More information can be found on www.elsevier.com. • Degraer S., Wittoeck J., Appeltans W., Cooreman K., Deprez T., Hillewaert H., Hostens K., Mees J., Vanden Berghe W. & Vincx M. (2006) The macrobenthos atlas of the Belgian part of the North Sea (book and CD-rom). Belgian Science Policy, D/2005/1191/3. 164 p. The macrobenthos atlas is a rich illustrated publication, with an overview of the most important macrobenthic organisms, living in and on the sea bottom of the Belgian part of the North Sea. Benthic organisms are not well known to the general public. Nevertheless, they form an important food source for many fish and bird species. In addition, they can be used as indicators for the ‘health’ of marine ecosystems. The first part gives an extended introduction on the role of the macrobenthos in the ecosystem, the sea bottom as habitat for the different benthic components, the impact of human activities (fisheries, sand extraction and eutrophication) on the benthic fauna, the availability of the density data (Macrobel), and the scientific research methodologies. The second part gives a detailed description of 53 macrobenthos species from the Belgian part of the North Sea (11 bivalve shellfish, 25 polychaete bristleworms, 12 small crustaceans, 4 echinoderms and the lancelet). Based on distribution maps, a clear picture is provided of the mid- term evolution (comparison of 1976-1986 with the period 1994-2001) of the occurrence of these species in the Belgian part of the North Sea. In addition to a short description of the species, illustrated with a clear photograph, the habitat preference for certain sediment types (median grain size of the sand and mud content) is represented. The book and CD-Rom are the main deliverables of the cooperative research project MACROBEL between Ghent University – Marine Biology Section, ILVO-Fisheries – Environmental Monitoring Section, and Flanders Marine Institute (VLIZ). Four scientists from ILVO-Fisheries (Environmental Monitoring Section) contributed to this book: Jan Wittoeck computerised the basis density data, Kris Cooreman was partly responsible for the project, Hans Hillewaert digitally processed the pictures of the organisms, and Kris Hostens contributed to the introduction and the final editing of the book. The book counts 164 pages. Both the book and CD-Rom were financially sponsored by the Belgian Science Policy, and are freely available at the different institutions. ISBN 90-810081-6-1. The macrobenthos atlas is also available in Dutch and French. The titles are ‘De macrobenthosatlas van het Belgisch deel van de Noordzee’ ISBN 90810081-5-3, and ‘l’Atlas du Macrobenthos de la partie Belge de la Mer du Nord’ ISBN 90-810081-7-X. More information can be found on www.belspo.be. • Luten J.B., Jacobsen C., Bekaert K., Sæbø A. & Oehlenschläger J. (Eds.) (2006) Seafood Research from Fish to Dish - Quality, Safety and Processing of Wild and Farmed Fish. Wageningen Academic Publishers, 567 p. The route that is covered before a fish product can finally be consumed by humans, is at least to be called a long one. Therefore, product quality, safety and food processing have always been important issues in seafood research. However, over the past years a marked shift occurred in the way consumers deal with food and health. Consequently, seafood research – traditionally mainly technological oriented – has changed directions, where also the habits and demands of the consumer are taken into account. In this book, scientists from different disciplines discuss the latest developments in seafood research, with special attention for the quality, safety, product technology of both wild and farmed fish, and the consumer’s perception of the whole process. The book consists of 52 papers divided into 8 chapters, dealing with nutrients and contaminants in fish products, as well as with the microbiological quality, freshness and methods to measure fish quality. Also, a chapter is spent on the knowledge of the consumers about seafood and the processing of all kinds of by-products of the fishery. 81 3.4 The Social Sciences Unit: new paradigms The book is the end-result of the 35th WEFTA meeting, which was organised in Ghent in 2005 by IMARES (NL) and ILVO-Fisheries, in combination with the yearly meeting of the European section of the AOCS. Karen Bekaert (ILVO-Fisheries, Product Technology Section) co-organised this international symposium and is co-editor of this book. Additionally, Karen Bekaert wrote the paper ‘Development of a Quality Index Method scheme to evaluate freshness of tub gurnard (Chelidonichthys lucernus)’. Three other scientists from ILVO-Fisheries (Environmental Monitoring Section), Marc Raemaekers, Sabrine Derveaux and Koen Parmentier, together wrote another paper for this book, entitled ‘Polychlorinated biphenyls and organochlor pesticides in brown shrimp (Crangon crangon) off the Belgian Continental Shelf’. The book counts 567 pages and costs 85 €. ISBN-10: 90-8686-005-2; ISBN-13: 978-90-8686-005-0. More information can be found on www.WageningenAcademic.com/seafood. The fourth ILVO pillar, the Social Sciences Unit (SSU), emerged from the former Centre for Agricultural Economics and will be, from 1 January 2007 on, enlarged with the former Flemish Policy Research Centre for Sustainable Agriculture. In an earlier stage, the unit was still assigned with the title “socio-economics”, but expectations soon proved to be much larger. Supported by external consultancy (WS Brussels) the persons and authorities involved defined a mission, research domains and researchers’ profiles for the new unit. In the course of 2006 brainstorming revealed a need for radical changes in paradigms, regarding for example the above-mentioned term “socio-economics”, a number of customary (and often used up) research methods and the governance structure. Point of departure is the specific context of agriculture and horticulture in Flanders. Moreover, the mission starts from the assumption (belief?) that agriculture will continue to contribute to Flemish economy and welfare. However, social and economic constraints force agriculture and horticulture to evolve towards a sustainable and competitive model. These changes can be coordinated and tuned through steering the decisionmaking of all actors involved, given their possibilities and limits. This kind of governance is an ever-lasting questioning process of searching, learning and experimentation. The role of scientific input in this process stands out clearly: it can help to improve decision-making. These basic assumptions are the driving factors to the SSU mission, which is stated as follows: to propose and to clarify, on a scientific basis, social choices concerning a sustainable and competitive agriculture and fisheries in Flanders. The term “choice” has to be considered as the most important of the mission and justifies the “right to exist” of the unit. The mission does not lay down who the decision-maker is, but the adjective “social” shows that the criteria concern various social actors. The main clients of the unit are: the Agriculture and Fisheries policy domain, the other ILVO units and the Flemish agricultural sector. Essentially, the mission is policy-supporting, which can be seen as an attracting force, bringing policy-makers and policy-support researchers closer together. The research model and policy-making process, however, do not share the same logics, which is a repulsive force. Attracting and repulsive forces create the arena, in which the research on choice clarification has to be integrated. The SSU will also support research and development and innovation diffusion through clarifying choices of actors involved in research (the three other ILVO units) and agricultural production. 82 The logic behind SSU’s mission is represented in figure 1. The research model reflects the basic assumptions, not only the above-mentioned client orientation, but also a thematic and a disciplinary orientation. The thematic orientation reflects the temporal and spatial context in which the social choices should be proposed and clarified. A set of research instruments (quantitative, qualitative, participatory and action-research methods) has to make choice clarification operational and leads to the research domain “integration”. The temporal dimension points to the development, through innovations, from a based on the sustainability concept and its three pillars, so integration then means clarifying choices with economic, social and ecological criteria. Vertical integration points to the various stages of the decision-making process: descriptive phase, evaluation of alternatives and presentation of policy options. The vertical integration aims at tuning these stages in a coherent and effective way. The “transition” research domain can be seen as a continuation of “Integration”, but differs from it through emphasising on choice clarification within larger and more Schematic presentation of the research model of the Social Sciences Unit current to a more sustainable situation. This leads to the research domain “transition”. Finally, the spatial-environmental dimension points to the specific regional context of high urbanisation and spatial competition in Flanders and leads to the research domain “Spatial facets”. complex systems in a long-term perspective. Basic assumptions on predictability and controllability are replaced by a more sophisticated framework, formed by cornerstones such as distributed intelligence and power, complexity and adaptive learning processes. To achieve a significant fulfilment of its mission, the unit has a disciplinary orientation that allows for, first, a longterm perspective integrative evaluation framework, second, picturing the systemic coherence between various domains of human activity and, third, exploring social changes as a continuous process of searching, learning, and experimentation. This can be summarised in the keywords transdisciplinary, systemic and prospective. Finally, the “spatial facets” research domain is oriented towards agricultural development within its specific spatial context. Although this research orientation can be seen as an extra dimension of integration, more specific research fields can be distinguished: As stated above, the research model generates three research domains: “integration”, transition” and “spatial facets”. “Integration” predominantly occurs across a horizontal and a vertical axis. The horizontal integration is · · · · quantitative research within the spatial planning arena; qualitative research within the stakeholder arena; research linked to the “integration” and “transition” research domains; research on the role of agriculture in sustainable regional-spatial and rural development. 83 3.5 Centre for Plant Diagnosis: in the spot The way agriculture is challenged by harmful organisms may show dramatic changes due to climate warming. Not only already existing diseases will increasingly be fuelled; but as climate dictates their occurrence, warm temperature diseases may in particular result in failing crop production. The last few years, bacterial stem rot, caused by Erwinia chrysanthemi, shows a remarkable progress in seed potato productions. It is a pathogen with subtropical properties and it affects various crops, e.g. corn on which it was diagnosed for the first time in Flanders in 2005. Diagnostic research at ILVO and field observations from the certification services of the Flemish Department of Agriculture confirmed the epidemic nature of bacterial stem rot in 2006 which is held responsible for a high number of rejections or declassifications of the seed potatoes produced in Flanders. Expertise and consultancy services from the bacteriology laboratory of the Centre for Plant Diagnosis (CPD) contributed to the management of the disease. In an early stage of the disease, bacterial stem rot is expressed by rotting of the mother tuber, resulting in wilting of the potato plants due to insufficient root development. Ink black stems, the typical symptom of the disease, develop later on. The proliferation of bacterial stem rot in seed potatoes was enhanced by the conducive weather conditions during the first six weeks of the crop in which subtropical temperatures were accompanied by thunderstorms which resulted in waterlogging on the potato fields. Erwinia chrysanthemi also performs well in soils with relatively low oxygen. There are no effective measures to control dissemination. The primary source of bacterial stem rot is presumably the planted seed itself which has become contaminated during the various previous cycles of field multiplication. Contamination mainly occurs during harvest and grading. It is known that one rotten tuber may contaminate 1000 84 kg of seed potatoes on the grader. We have obtained evidence that after tuber contamination the pathogen preferentially persists at the apical buds which is the ideal spot to initiate new infections. CPD provides a sensitive method for detection of Erwinia chrysanthemi based on specific PCR tests after selective enrichment in polypectate medium. The test is however not quantitative and does not allow to determine the percentage of contaminated tubers in a seed potato lot. For several years already, the tissue culture derived nuclear material for seed potato production is analysed. Infections or contaminations of Erwinia chrysanthemi were not detected. Peroxide decontamination of seed potato tubers was tried but found to be ineffective. The effects of refrigeration on the persistence of the pathogen on seed potato tubers are now being examined. 3.6 Measuring residual nitrates in soils: elevated nitrogen utilisation as solution? Farmers may have a management agreement with the Flemish government for reducing nitrogen fertilisation in nitrate-vulnerable zones. These management agreements are a measure for additional actions in the framework of reducing water pollution with nitrates in return for a financial compensation. On demand of individual farmers, Agrolab samples selected parcels and analyses the samples for residual nitrates in the framework of these management agreements. 279, 194, 170 and 161 parcels were sampled at 3 depths and residual nitrate-nitrogen was measured in 2002, 2003, 2004 and 2005, respectively. These parcels are primarily located in the Flemish sandy region, more specifically in the North of the EastFlanders province. In these consecutive sampling years, residual nitrate for respectively 50, 27, 24 and 6% of the parcels exceeded the standard of 90 kg of nitrate-nitrogen per ha for the 0-90 cm soil depth. For 11, 4, 5, and 0% of the parcels a residual concentration higher than 180 kg of nitrate-nitrogen per ha was measured. This data set is not representative for the studied area, but allows for determining the temporal trend in residual nitrate for these parcels sampled in consecutive years. 131 parcels were all sampled in 2002, 2003 and 2004. The average residual nitrate in 2002 for this data set was significantly higher than for sampling in 2003 and 2004 when compared with ANOVA. In 2002 the average nitrate residue was 80 kg of nitrate-nitrogen per ha, while values for 2003 and 2004 were 53 and 60 kg of nitrate-nitrogen per ha. 111 parcels were sampled consecutively in 2003, 2004 and 2005. The average nitrate residue in 2004 (53 kg of nitrate-nitrogen per ha) was significantly higher than in 2005 (42 kg of nitrate-nitrogen per ha). A good crop development requires sufficient plant-available oligo-elements. A balanced fertilisation with attention for all essential macro- and oligo-elements may result in higher yields and thus a higher N uptake by the crops. In a study financed by the Agricultural Centre for Fodder Crops, the effects of several combinations of sulphur and selenium fertilisers on grass production and grass quality were studied in cooperation with the Soil Service of Belgium. Two experimental sites (Hoogstraten and Merelbeke) on mowing fields were fertilised with combinations of potassium sulphate and selenium-containing nitrogen fertilisers. The Merelbeke site was monitored by ILVO. Sefertilisation was sufficient for an optimal Se-concentration in the grass at Hoogstraten, and for the first and second cut at the Merelbeke site. S-fertilisation resulted in a higher crop yield and a higher N export in the grass. Higher S-fertilisation caused higher S-concentrations in the grass, in combination with a lower Se- and Cu-uptake in the grass. A higher crop yield combined with a better N uptake due to a balanced fertilisation can result in reduced nitrate leaching. The residual nitrate concentrations will be measured for the 0-90 cm soil depth in fall 2006. For comparison of the effects of compost fertilising with a more regular application of stable manure or slurry, a medium-term fertilisation experiment was initiated in 2005. The experiment consists of two compost objects, i.e. single and double dose farm compost. The farm compost was made at ILVO. Besides a stable manure object, 2 slurry objects were incorporated in the experiment, with one object in which green compost was added. The green compost was made at a VLACO-certified compost installation. The organic matter-input was equalised for the single dose farm compost object, the stable manure object and the object with slurry combined with green compost. As compost is preferentially incorporated superficially, 2 soil treatments were compared: minimal soil tillage was used for the compost treatments, while more intensive cultivation was applied for objects with stable manure and slurry. The 4-year crop rotation is maize – potatoes – grain – red clover. In 2006 potatoes were grown. Besides the difference in residual nitrate between treatments, also differences in soil structure, soil biology, organic matter content, soil nutrient balance, crop development and pest pressure are monitored. Per replicate the residual amount nitrate-nitrogen was measured at 3 depths (0-30, 30-60 and 60-90 cm). For all treatments, the residual nitrogen at the end of the growing season 2005 and 2006 was lower 85 than 90 kg of nitrate-nitrogen per ha for the 0-90 cm soil depth. In 2006 significant differences (p < 0.01) between treatments were detected by ANOVA. Both farm compost application rates (on average 48 kg of nitrate-nitrogen per ha) resulted in significantly lower residual nitrates than the 3 other objects (on average 73 kg of nitrate-nitrogen per ha). For all treatments, nitrate concentrations in the 030 cm soil layer were significantly higher than for the 3060 cm layer, which in turn were significantly higher than in the 60-90 cm layer. These residual nitrate concentrations should be assessed in combination with the crop yield. Potato production on organic parcels with single (C) and double (2C) dose of farm compost, farmyard manure (SM) and slurry (DM) (situation at end of June 2006) 86 3.7 Cooperation of ILVO-T&V with Flanders’ FOOD projects Flanders’ FOOD is an initiative of FEVIA Flanders, IWT and the Flemish government, with the mission of “the consolidation of the competitiveness of the Flemish Food Industry by directed stimulation and implementation of INNOVATION”. Flanders’ FOOD aims to be a unique knowledge and innovation centre and a central information service for the Flemish Food Industry where the industry can be served with their scientific and technological questions and needs. The general goal is to consolidate, to broaden and to deepen the innovation process in the food industry by development, dissemination and valorisation of knowledge. The target group of Flanders’ FOOD consists of small and medium enterprises as well as large concerns with a production unit and/or R&D centre in Flanders. be carried out in collaboration with Prof. K. Dewettinck (Department of Food Safety and Food Quality, Ghent University) and MSc Tony Ruyssen (Laboratory of Dairy Technology, Association University Ghent). In the second project the psychrotrophic pathogenic sporeforming microbiota, and more specifically Bacillus cereus, will be investigated. In the determination of the shelf life of ready-to-eat meals, it is important to be aware of the possible development of this type of micro-organisms which can survive the applied heating step. More specifically, the effectiveness of heating in suppressing these micro-organisms as well as their characteristics (toxin production, biodiversity) will be investigated. This project will be conducted in collaboration with Prof. F. Devlieghere (Department of Food Safety and Food Quality, Ghent University). Definitely, Flanders’ FOOD is the link between the food industry and the scientific centres (universities and other scientific institutes). Based of the needs of the industry research has been initiated by Flanders’ FOOD by starting up in several Flemish scientific centres a number of selected collective and application-directed projects. ILVO-T&V participates in two such projects, respectively sugar reduction and/or sugar substitution in sour and neutral drinks and in ice cream, and prolongation of the microbial shelf life of foodstuffs. The first project investigates how and to what extent sugar reduction and/or sugar substitution could be realised in several model systems such as sour and neutral drinks and in high viscous liquids such as ice cream mixes. It is the aim to replace the sugars in the different model systems with respect to all of its functionalities necessary to maintain the product properties as much as possible. Proposals for solutions that approach or imitate as much as possible the reference product will be generated and information and/or guidelines will be provided with respect to process conditions and shelf life of the products. This research will Electron microscopic view of a spore of Bacillus cereus 87 3.8 Role of ILVO-T&V in the Belgian National Reference Laboratory (NRL) Consortium for GMOs The production of genetically modified (GM) plants and the market introduction of food and feed products that are derived from GMOs or contain GMOs, must be in compliance with a strict European regulatory framework. The new regulations – “food and feed” regulation 1829/2003/EC and regulation 1830/2003/EC on “traceability and labelling” – enforce that all food and feed products containing GMOs and derived thereof, over the whole chain, must be labelled above a threshold of 0.9% per single ingredient. In addition, for non-authorised GMOs that yet received a positive risk evaluation from the Commission, labelling is obligatory above a relative concentration of 0.5%. Due to this firm legislation on the one hand, and the increasing complexity of GMOs on the other hand, the availability of precise and accurate analytical methods for unique identification and quantification of GMOs is crucial. For GMO control, it is important that governments can rely upon specialised and competent laboratories. Within Regulation 1829/2003/EC, the EU foresees in the establishment of a Community Reference Laboratory (CRL) for GMOs, established in the JRC in Ispra, Italy. In its functioning, the CRL is sustained broadly by the European Network of GMO Laboratories (ENGL), involved with the development of GMO control strategies. The same regulation also describes that EU member states can establish National Reference Laboratories (NRLs) for implementation of the GMO regulations at the national level. The objective of these NRLs for GMOs is to support the CRL and the ENGL, by means of e.g. participation in diverse working and discussion groups. In Belgium, the FPS Health, Food Chain Safety and Environment, and the Federal Agency for the Safety of the Food Chain (FASFC), are the responsible authorities for regulation and control of the food chain, including GMOs. Since 1 July 2006, the NRL for GMOs is established by law as a consortium of three laboratories: the Scientific Institute of Public Health (IPH), the Centre wallon de Recherches agronomiques (CRA-W) and ILVO-T&V. The mission of the NRL is to solve technological problems and to support the application and development of new methods and technologies, reference materials and calibrants for the assessment of GMOs. More in particular, the main tasks of the NRL are the following: 88 • • • • • • • • • To technologically-scientifically carry out GMO analyses under accredited conditions (ISO 17025) and to continuously update the analytical methods; To participate in international interlaboratory trials; To organise ring trials for routine laboratories at the national level, focusing on the implementation of harmonised interlaboratory tests; To ensure efficient and effective communication of documentation and information towards the routine laboratories; To support the quality of external routine laboratories by technically sustaining them as well as communicating the needed knowledge (e.g. by means of a website); To inform the FASFC on the interpretation of analytical results; To maintain contacts with the ENGL and to transfer related information to the FASFC as well as external laboratories; To guide and sustain research and monitoring projects in the involved domain; To actively sustain the FASFC, scientifically and technically, in the case of incidents or crises. 3.9 PreventAgri Vorming/Formation encourages safety in agriculture and horticulture Since already several years, the federal project “PreventAgri Vorming/Formation” is involved in a safety and health project in the Belgian agriculture and horticulture. The prevention of accidents at work consists of three parts: the circulation of information, sensitisation and education. The project was founded in November 2001 and is financially supported by the Federal Public Service: Employment, Labour and Social Dialogue and the European Social Fund. At that time the project was fully coordinated by the University of Liège. Since July 2006 the coordination of the Flemish staff members is carried out by ILVO-T&VAT. The Walloon staff members are since then coordinated by the Mission Wallonne des Secteurs Verts (MWSV). Collection and circulation of information (sensitisation) Between 2001-2003, plenty of useful information was collected with the aim of publishing different brochures, each covering a specific topic of safety or health in Belgian agriculture and horticulture. Topics discussed included among others: statistics of accidents, safe working with tractors, machines or animals, handling of dangerous products, the exposure to physical agents (vibration, dust, noise), etc. Furthermore, PreventAgri Vorming/Formation publishes a monthly e-newsletter that is mailed to about 500 interested persons, in which an actual ‘safety’ topic is treated. Education Since 2003 there is a great demand by the sector for education. Last year some 1,300 interested persons were reached in some hundred training sessions. The purpose of these free trainings is to initiate the creation of safer and healthier working conditions in agriculture and horticulture. During these sessions, interactive working is promoted as much as possible because ‘safety’ is a topic for which enthusiasm and belief is needed, before it can become effective at work. The training sessions encompass a basic formation wherein the methodology of a risk analysis is explained and the major risks on a farm are discussed. We suggest prevention measures and stimulate people to participate actively in the thinking process. Besides this basic formation, other topics can be treated like ‘Safe working with dangerous products’, ‘Safe working with machines’, ‘Traffic safety’, ‘Prevention of back complaints’, ‘Zoonosis’, ‘Fire prevention’, ‘Formation of teachers’. Finally, some specific sector-related topics are available (cattle, poultry, pigs), where an inventory is also made of the specific risks occurring in those sectors (dust production in the poultry and pig sector, exposure to noise pollution, back complaints, unpredictable animal behaviour). Furthermore, appropriate prevention measures are discussed. Risk analysis Upon explicit request, PreventAgri Vorming/Formation performs a free risk analysis on the farm. In this way, the people working in an agricultural or horticultural enterprise are informed about the present risks and in the end, appropriate measures can be proposed and discussed. This is important, because in the long term some possible risks are ignored and forgotten because they have become familiar. To avoid this, the view of an outsider can arouse renewed awareness for these risks. On the other hand, the visits to those farms always yield plenty of practical experience for the project. Moreover, many farm managers already thought about safety on their farm and worked out some unique safety measures. These can then be suggested to other farm managers. Contact www.preventagri.be [email protected] [email protected] Preventagri Vorming Burg. Van Gansberghelaan 115 B-9820 Merelbeke 89 90 4.1 Reference laboratory for research on animal nutrition and the nutritional value of animal products The activities of the reference lab fit in the policy-supporting tasks of the Animal Science Unit, located at Melle, being: - Research for a better feed evaluation and for fine- tuning the nutrient requirements of cattle, pigs, poultry and rabbits - Research into functional animal nutrition which should contribute to animal end-products with a higher health value - Applied research in the framework of sustainable, consumer-directed and socially acceptable animal husbandry In the framework of research on nutrition physiology analyses are carried out on faeces and urine of cattle, sheep, pigs, poultry and rabbits as well as on rumen fluid and intestinal contents. Finally, the chemical composition and fatty acid composition of the animal products milk, meat and eggs are determined. Also the sensory characteristics of meat like tenderness, colour and waterbinding-capacity can be investigated. 4. The laboratory works according to the ISO 17025 standard. Analyses are carried out on animal feeds (compound feeds, feedstuffs, roughages): - chemical composition: moisture, crude ash, crude protein, crude fat, crude fibre, NDF/ADF/ADL, starch, sugars, calcium, phosphorus, gross energy - fermentation characteristics (silages): pH, ammonia fraction, lactic acid, volatile fatty acids, alcohols - composition of higher fatty acids - in vitro digestibility of the organic matter and of the cell walls - cumulative gas production by in vitro incubation as measure for digestion rate - NIRS-analysis for a fast screening of the chemical com position Public service Furthermore, research is carried out on behalf of the feed industry, the analytical laboratories and the sector of animal husbandry. 91 4.2 Social Sciences Services provided by the Social Sciences Unit include: Spin-off towards monitoring • • • Contribution to the annual Flemish environmental reporting (MIRA), chapters Agriculture and Eutrophication and drawing up the corresponding background documents (www.milieurapport.be) Delivery of indicators to the OECD, e.g. the soil nutrient balance Delivery of indicators concerning agriculture, environment and rural development to EUROSTAT Advice and services Advice concerning policy issues, usually on topical matters (in the past recommendations have been formulated concerning organic farming, the manure policy, the sugar policy and rural development) Feedback groups and refereeing • • • Participation in diverse stakeholder and feedback groups Refereeing of several chapters in MIRA (environmental report), NARA (nature report) and LARA (agricultural report) Refereeing of policy preparatory documents, among others concerning the themes mentioned above. 4.3 Centre for Diagnosis of Plant Pests The Centre for Diagnosis of Plant Pests (CDPP) constitutes an operational unit of four plant health laboratories, viz. bacteriology, entomology/acarology, mycology and nematology within the unit Plant-Crop Protection which have joined their expertise in diagnosis and detection of plant pests. Samples of plants and plant products, soil and water as well as imported plant material, stored foodstuffs and materials from houses or other buildings can be submitted for analysis of harmful organisms. Bacteria, fungi, insects and mites, nematodes are accurately detected, isolated and identified using a wide range of conventional as well as innovative techniques. The CDPP offers stateof-the-art services to growers, inspection and extension organisations, research institutes, government agencies, private companies and persons. The laboratory of bacteriology starts diagnostic analysis by dilution-plating infected plant tissues to verify if and to which extent plant pathogenic bacteria are involved in the disease symptoms. Many of the harmful bacteria encountered in diagnosis can be quickly identified with highly specific antibody and PCR tests complemented with a pathogenicity test to prove that the isolated pathogen is really the cause of the disease (Koch’s postulates). However, sometimes it is not straightforward to identify the isolated bacterial pathogen so that phenotype tests are required too. Detection of latent infection in plant material such as potato tubers and seeds and contamination in various matrixes such as water, soil and waste products of transformation processes involve selective plating, serological testing and DNA analysis, in particular after selective enrichment of the target pathogen. Specific plant pathogenic bacteria can be characterised by a molecular fingerprint which may give indications on introduction or distribution. Internationally recognised and standardised detection protocols (EU, EPPO, ISTA) are available for specific bacterial pathogens in potato tubers, seeds and planting material. The laboratory of entomology and acarology provides expertise in detection and identification of harmful insects and mites in crops, stored foodstuffs, in houses and other buildings, public and private gardens, imported and exported plant material. Insects and mites can be identified directly by examination under a binocular microscope or after extraction from the sample by incubation in Berlese funnels (isolation by light and temperature) or, specifically for mites, by a centrifugation/flotation method. The extracted organisms are conserved in alcohol solution for further determination which is performed by examination of morphological properties available in identification 92 keys. A large and extensive collection with a wide diversity of insect and mite specimens is available for reference purposes. In the laboratory of mycology, fungi that are obligate parasites are directly identified on the infected plant tissue by microscopic examination, if necessary after incubation. Other plant pathogenic fungi from diseased plant tissues are determined after isolation on culture media or incubation in a buffered salt solution. Fungal material, including spores and hyphae, are collected from water samples by membrane filtration. Qualitative and (semi-)quantitative analysis by selective plating or by molecular detection, viz. real-time PCR, reveals the presence of specific fungi and, if detected, allows estimation of their density in the sample. This application is used frequently to monitor the performance of disinfection equipment (UV lamps, slow sand filtration) and of recycling nutrient solutions in hydroponic systems. A particular test for analysis of water samples is the leaf bait test in which Rhododendron leaves in a mesh bag are deposited for three to five days in the water sample or in the water tank. Spores of Pythium or Phytophthora are attracted by the leaves and will finally cause infections. The leaves are sent to the lab where the leaf lesions are analysed by plating on selective culture media. The developing fungi are identified microscopically by their morphological characteristics or by molecular analysis. Appropriate isolation techniques are also practised in the laboratory of nematology to extract nematodes from soil, water, various culture substrates, wood and plant tissues. A unique equipment has been constructed to obtain free living nematodes from soil or roots. Using the principle of zonal centrifugation a sample is processed within a few minutes followed by isolation of the nematode specimen which is identified by microscopic examination of its morphological properties. Nematode identification often implies measurement of small morphological details to differentiate accurately between species and this analysis is facilitated by image-processing. Moreover, relevant details are stored as evidence and as reference material which is very useful for plant quarantine nematodes. Molecular identification is performed for species which cannot reliably be differentiated by morphological analysis. A particular test is provided for the potato cyst nematode. Determination of the viability of the isolated nematode cysts, which involves the counting of the number of eggs and juveniles, is used to assist phytosanitary decisions. after a successful pre-audit by the Belgian Accreditation Board (BELAC) in October. Pending the finalisation of laboratory alterations to meet bio-security requirements, the acquisition of the accreditation certificate for each of the four laboratories is acknowledged as official recognition of the technical competence and of the objectivity in performing a wide range of phytosanitary tests using standardised and validated protocols. Accreditation also forces CDPP to engage in a process of continuous improvement and innovation which implies that the tests are performed according to state-of-the-art methodologies and by motivated and conscientious staff which will increase confidence of government services and private operators in the results of the analysis. The acquisition of the ISO accreditation is a strategic objective for CDPP and targets capacity building for further expansion. The analysis of large numbers of samples within the proposed time delay requires a permanent critical evaluation of the daily activities. The ILVO phytosanitary laboratories which perform according to the ISO quality system will be a powerful instrument for the government for making underpinned policy decisions and an important tool to resolve trade arrangements or disputes. The requirements on organisation and performance will also contribute to assure the quality and health of the agricultural and horticultural production in Flanders and to manage crisis situations. Because ISO accreditation is unequivocally identified, it will consolidate and increase confidence of the producer as well as the consumer in a Flemish quality product. ISO 17025 accreditation should allow CDPP to take a leading position in diagnostic research and associated services on the European and the international plant health forum. CDPP is in the final phase of obtaining ISO 17025 accreditation for a number of diagnostic plant pest protocols 93 4.4 Varieties developed by ILVOPlant - Cultivars on variety lists and commercialised Seed multiplied Perennial ryegrass – fodder type diploïd - Achat - Isabel - Melland - Melpico - Merbo - Merganda - Merks - Paddok - Plenty - Odessa - Rebecca - Ritz - Vigor tetraploïd - Ernesto - Floris - Graciosa - Meradonna - Merkator - Merkem - Merlinda - Pandora - Pomerol - Roy Perennial ryegrass – turf type - Olano - Oxiana - Relon Italian ryegrass diploïd - Adin - Bellem - Belluna - Davinci - Lemtal - Melcasso - Melchior - Melclips - Meribel - Merode - Mertaki - Meryl - Muriello - Nadine - Prospect - Romeldo tetraploïd - Gemini - Fedra - Melquatro - Meritra - Meroa - Racine - Salomé Hybrid ryegrass 94 - Hymer - Lemsor - Merini Westerwold ryegrass diploïd tetraploïd - Melworld - Mendoza - Lemnos - Melmondo Meadow fescue - Merifest Timothy - Anjo - Comer - Dolina - Erecta - Tibor Red fescue - Nevski - Rolf Red clover - Global - Lemmon - Mercury - Merian - Merviot - Rotra - Violetta White clover - Merida - Merwi - Merlyn Chicory - Arancha - Arioso - Belcanto - Crescendo - Hera - Melci - Vivace Stubble turnips - Durmelander - Dynamo - Leielander Fodder beets - Adagio - Apex - Bolero - Dana - Gonda - Ribondo - Romeo - Uno White mustard - Chacha - Flamenco - Meringue - Polka - Rumba - Salsa - Solea - Swing Fodder radish - Brutus - Cassius - Dux - Lucas - Nero - Sirius - Sixtus Forage rape - Dino - Napoleon - Wilma Parsley - Mersil Scorzonera - Antonia - Keukenfee Leek - Makostar Vegetatively multiplied Roses - Adolf Papeleu - André Brichet - Annelies - Anton Van Dijk - Balduinus - Benoit Friart - Celientje - Cera - Dream - Floranje - Florizel - Godelieve - Gold Cup - Gomery - Graaf van Vlaanderen - Hertog van Brabant - Jacky’s Favorite - Joke - Kanegem - Kasteel van Ooidonk - Koksijde- Liparfum - Lysa - Margriet Hermans - Marie Louise Velge - Melglory - Melgold - Melrose - Michelle d’Hoop - Nele - Pink Kanegem - Prinses Astrid - Prinses Mathilde - Professor Boesman - Rafael Braeckman - Rivierenhof - Rosarium Den Blakken - Sabine Cut roses Bromelia Hibiscus - Showy gold - Slot van Laarne - Toporanje - Ville du Roeulx - Wettra - White Symphonie - Windekind - Païline - Cathy - Diabolo - Fernanda - Festival - Regine de Ligne - Romero - Melmauve - Melroze - Melwhite Malus- DvP Obel/Red Obelisk® - Pompom Azalea- Cheops - Cupideau - Directeur Van Slycken - Gilbert Mullie - Lara - Laura Ashley - Lara Rood - Mevr. André Heungens - Mevr. Jozef Heursel - Mevr. Marcel Vanbelle - Mevr. Roger De Loose - Mevr. Van Eetvelde - Mistral - Phoenix - Prinses Claire - Roger Raveel - Rolinda - Roxane - Roxette - Schuman - Vinivi Ligustrum - Melgreen/Green Century® - Melblack Begonia - DvP - Optima Savanna - Optima Taiga Chamaecyparis - Melgold Prunus - Melred - Melred Weeping - Melstar Hydrangea paniculata - DvP Pinky/Pinky-Winky® 95 4.5 TAD FarmCOMPOST The consultancy service TAD FarmCOMPOST provides information and advice on farm composting and the use of compost and compost tea for the Flemish agricultural sector. The project is financed by IWT and Symbios, an association of agricultural organisations. On 11 January 2006 FarmCOMPOST organised a seminar ‘Agriculture has a future: even with reduced fertilisation and reduced pesticide use’. The central theme was ‘crop type directed soil management with living inputs: compost and compost tea’. The guest speaker was Dr. Elaine Ingham of the Sustainable Studies Institute, Corvallis, Oregon, United States of America. A hundred participants, civil servants, consultants, researchers and growers were captivated by her explanation about characterising and interpreting cultivation systems based on the soil foodweb and measures that act upon the soil foodweb. A crop- directed soil management that introduces the correct soil biology, results in a sound crop and a high product quality. The next day, 12 January, a second presentation was made for a more limited public. The theme then was ‘Application of compost tea in hydroponics’. Aspects as working mechanisms of compost tea, compost tea technology and application were treated. In 2006, the accent for TAD FarmCOMPOST was on orientating experiments on farms concerning the use of compost and compost tea. An experiment that started in 2005 and continued in 2006 has to reveal whether fertilisation on begonia parcels, more specifically the nature of the organic basic fertilisation influences tuber quality. A fertilisation trial with the following objects was conducted on two parcels: stable manure plus mineral fertiliser versus fungal dominant CMC-compost plus organic fertiliser. The compost objects received no extra fertilisation during the growing season contrary to the objects with stable manure. There was no difference in crop protection measures, water supply and soil tillage between compost and stable manure objects. During the first growing season (2005) and the resulting tuber harvest some striking results were already observed. During the cultivation period the epiphytic infection of Xanthomonas axonopodis pv. Begoniae was determined, 96 the bacteria causing leaf spot disease. There was a significant lower infection with the compost objects. Tuber quality was evaluated on the basis of the percentage of dry rot during the conservation period and the percentage of tubers not sprouting after the conservation period. In the case of the begonia type ‘Dubbel Zalm’, which is sensitive to conservation rot, a more than double percentage loss is assessed for the stable manure object in comparison with the compost object. In 2006, an experiment was set up at the Proefcentrum Hoogstraten in cooperation with TAD FarmCOMPOST and the Acterra company concerning the addition of CMC-compost to the growing medium and different fertilisation variants for a delayed and continued (till May 2007) strawberry crop. This is an extension of the research that was carried out at ILVO during three growing seasons, 2003-2005, where in the case of a compost-based substrate a high suppression of powdery mildew, grey mould, spider mites and aphids was observed. With the assistance of FarmCOMPOST some compost tea applications were tested in Flanders in 2005 and 2006. Compost tea appeared effective against mildew in tree nursery on sensitive species, hawthorn (Crataegus monogyna) and field maple (Acer campestre). A weekly application of compost tea on a tomato crop in hydroponics prevented to a high extent Botrytis cinerea infection (stem rot). After starting the treatment the wounds on the already infected stems dried out preventing plant losses. An explanation for this success is found in literature. The phyllosphere harbours a complex microbial community in which fungi occupy a predominant place. It is likely that the food resources on the leaf surface are abundant enough to sustain a large and diversified number of fungi with different attributes. All fungi that have a saprophytic and epiphytic life phase will invariably have to compete against other fungi for the somewhat limited nutrients on the leaf surface. Hence, the use of saprophytic fungi as biocontrol agents is a logical strategy. They can colonise rapidly host debris that would otherwise serve as food source for plant pathogens. The infection by Botrytis cinerea on a tomato crop is starting on necrotic tissue of the stumps of the stems of the removed leaves. A fertilisation advice by FarmCOMPOST is based on a nutrient balance and a soil foodweb analysis. Since 2006, fertilisation is evaluated and adapted on the basis of plant juice analyses. Different parameters are determined. The brix gives an indication of the amount of solutes in the plant juice. The optimum pH-H2O is 6.2. Also the electrical conductivity (salts in solution) is determined, and separately the elements K+, NO3- en Na+. This strategy was successfully executed on different parcels for various crops (lettuce, fennel, hop, tomato, cucumber and pepper). . On 16 September the Acterra company organised a demonstration on farm composting on the farm ‘Het Zilverleen’ in Alveringem. They use a self-driving compost turning machine for contract work. FarmCOMPOST assisted the composting operations at the demonstration site and gave some explanation during the visit. What the FarmCOMPOST project has shown is that by tuning the cultivation measures to the suitable microbiology, the crop result can be improved dramatically. Compost and compost tea are the outstanding means therefore. Dried wound stem rot infection on tomato plants by using compost tea Trial ILVO-Proefcentrum Hoogstraten: strawberry on compost-based substrate 97 4.6 Variety Testing – Post-control trials - Seed Testing Laboratory In the context of a mandated assignment of the Agency for Agriculture and Fisheries - Product Quality Management Division, ILVO-Plant-TO gives scientific, technical and logistic support for the execution of the EU directives concerning the national variety catalogue of agricultural crops (Directive 2002/53/EU). In case of agricultural crops, a new cultivar can only be traded when it is registered on the Belgian or European variety catalogue. A cultivar which is admitted on a national variety catalogue of a EU-country automatically enters the European catalogue after several months (Directive 70/457 en 98/95). A new cultivar is admitted for inscription on the Belgian variety catalogue when it has an approved name, is sufficiently distinct, uniform and stable (DUS), and has a sufficient value for cultivation and use (VCU). To evaluate the DUS and VCU, a new cultivar is tested during 2 to 4 years in comparison with standard varieties, i.e. Variety Testing. The protocols for the DUS and VCU trials are put forward by the Technical Interregional Working group (TIW). DUS-trials ILVO-PlantTO executes the DUS-trials for chicory and fodder beets, according to the UPOV-criteria (Union pour la Protection des Obtentions Végétales). For other crops, there are bilateral agreements, e.g. with France for maize. At this moment, 2 fodder beet varieties (against 15 standard varieties) and 4 chicory varieties (against 21 standard varieties) are tested in the DUS-trials. A cultivar is sufficiently distinct when it distinguishes itself from any other variety registered in the EU or in trial by one or more important perceptible characteristics, which are based on: - morphology, e.g. root length and shape for chicory - colour, e.g. colour of the root for fodder beets, colour of the flower for chicory - physiology, e.g. inulin content for chicory - disease resistance A cultivar is sufficiently uniform when all the plants of the cultivar are similar or genetic identical for all considered characteristics, taking into account the method of reproduction. A new cultivar should be as homogeneous as all comparable varieties. 98 A cultivar is sufficiently stable when, after repeated reproductions, it still is in agreement with its description. VCU-trials ILVO-Plant-TO executes the VCU-trials for each agricultural crop when there is a new requirement for registration, except for sugar beets. In 2006 trials were carried out for silage maize and corn (123 varieties in trial), ryegrasses (37 varieties in trial), white clover (1 variety in trial), fodder beet (2 varieties in trial), chicory (4 varieties in trial), flax (4 varieties in trial) and cereals (28 varieties in trial). A cultivar has a sufficient value for cultivation and use when it includes a clear improvement compared to the current cultivars either for the cultivation, or for the valorisation of the harvest, or for the obtained products. Sometimes a lower level of certain characteristics (e.g. yield) can be compensated by specific favourable characteristics like disease resistance. At this moment, there are fodder beet cultivars in trial with a possible resistance against rhizoctonia or rhizomania. Depending on the species, 6 to 10 trials are laid out at different locations in Flanders (Merelbeke, Geel, Poperinge, Bassevelde) and the Walloon Region (executed by CRA-Gembloux). In the trials the new cultivars are compared with standard varieties (i.e. the current best varieties in the commercial circuit) according to beforehand determined criteria using a weighting factor. The new cultivar is admitted for inscription on the Belgian variety catalogue when the results of the VCU and DUS trials are positive and when it has an approved name. An important characteristic for VCU-research is the dry matter yield, together with some specific characteristics for each crop, for example: - silage maize and corn: digestibility, lodging and stalk rot - chicory: inulin-content - ryegrasses: rust resistance and persistence - fodder beets: tare - flax: fibre content Annually on average 10% of the tested varieties enter the national variety catalogue. By setting higher requirements for the standard varieties and by using criteria based on sustainability (quality, disease resistance and harvest security) only the best varieties are admitted for inscription to the national catalogue. In 2006 the following criteria were reviewed: - Review of the criteria for silage maize: number of rows, harvest date, precocity, digestibility - International cooperation for flax: comparison of the trial protocols of Belgium and The Netherlands, and working out a proposal for an international network. Post-control trials and Seed Testing Laboratory The following activities were set up for the executive control tasks in connection with the legal quality control of plant material that concerns the whole chain, from testing a new variety to control of the seed production and postcontrol of seed multiplications: - Official pre- and post-control trials for grasses, flax and potatoes - Supporting activities to the Seed Testing Laboratory. 99 4.7 Addition of accredited laboratory analyses in the framework of food authenticity and food safety The Quality Division of the research areas Food Safety and Product Quality and Innovation with its accredited laboratories forms an excellent tool for the food sector and the government. Since 26 September 1995, the laboratories of the Quality Division have the competence to perform their analyses according to BELTEST/BELAC criteria. The functioning of the division is in accordance with the NBN EN ISO/ IEC 17025:2005 standard and the ISO/IEC Guide 431:1997. More than 50 accredited analyses and 6 types of proficiency studies are performed and organised in four different laboratories. The chromatographic laboratory In the chromatographic laboratory, the following analyses are performed under accreditation: determination of aflatoxin M1 in milk, milk powder, yoghurt and cheese by means of immunoaffinity clean-up followed by LC-fluorescence detection - determination of PCBs in milk and milk products and in eggs and egg products by gas chromatography-mass spectrometric detection - determination of chemical coccidiostats in eggs and meat by LC-MS/MS - determination of β-lactam antibiotics in milk by LC-MS/ MS - determination of benzimidazoles in milk by LC-MS/ MS - determination of sulphonamides in honey by LCMS/MS - determination of macrocyclic lactones in milk by LC-fluorescence. In 2006, the scope of accredited analyses has been extended. In the chromatographic laboratory an extension was obtained for the determination of coccidiostats in meat by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two new methods were accredited, namely the determination of macrocyclic lactones in milk by means of LC-fluorescence detection and the determination of sulphonamides in honey by means of LC-MS/ MS. In the laboratory for physical and chemical analyses and the organisation of ring trials, the analysis of alkaline phosphatase and the organisation of an additional ring trial (determination of fat in cream) were added to the scope. Finally, the scope of the microbiological and GMO laboratory was extended in 2006 with the detection of genetically modified (GM) canola and the event-specific detection of GMOs in soybean, maize, and canola. For the latter, a flexible scope was obtained. The laboratory for physical and chemical analyses and the organisation of ring trials This laboratory performs physical and chemical analyses on milk and/or milk products. It concerns the determination of fat, protein, NPN, dry matter, density, freezing point, disinfectants and the analysis of alkaline phosphatase. Comparative studies are organised to give scientific guidance to the Belgian Milk Control laboratories and the Belgian dairy industry. The comparative studies are performed for the determination of the quality and the composition of milk (cfr. Royal Decree of 3 September 2000 concerning the recognition of the interprofessional control laboratories for the determination of the quality and the composition of milk), according to ISO/IEC Guide 43-1: 100 for identification of GMO events in all solid matrices and derived products, the laboratory is allowed to detect new events, listed in the CRL register of EU-authorised events, according to the CRL official detection methods. The laboratory for the detection of antibiotics 1997. The ring trials for the industrial labs are organised for the following parameters: total flora, coliforms, freezing point, inhibitory substances, fat and protein content on raw milk and determination of fat on cream. The microbiological and GMO laboratory Twelve of the fourteen microbiological analyses are performed on all foodstuffs: total (an)aerobic flora or sporeformers, coliforms, β-glucuronidase positive Escherichia coli, Enterobacteriaceae, Staphylococcus aureus, yeasts and moulds, Salmonella, Listeria spp., Listeria monocytogenes, sulphite-reducing anaerobic flora, Clostridium perfringens, Campylobacter, Bacillus cereus and E. coli O157. Yoghurt is examined on the presence and quantity of Lactobacillus bulgaricus and Streptococcus thermophilus. Enterotoxins from Staphylococcus aureus are detected in milk and milk products. In this laboratory 17 accredited analyses are performed. The matrices concern milk and milk products, honey and bee products, eggs, meat and meat products, fishery- and aquaculture products and kidney. In milk and milk products, residues of antibiotics (β-lactams, tetracyclines, quinolones, chloramphenicol, etc.) and sulphonamides can be detected. Honey and bee products can be screened on the presence of streptomycins, tetracyclines, chloramphenicol and sulphonamides. The detection of chloramphenicol in meat (and meat products) and in fishery and aquaculture products is also accredited. Finally, the laboratory has accreditation for the detection of sulphonamides in eggs and the screening of kidney for inhibitory substances. The complete scope of accredited analyses can be found on our Internet site http://www.ilvo.vlaanderen.be/T&V/ documents/Scope_accreditatie.pdf The scope of this laboratory also includes qualitative and quantitative analysis of GMOs in soybean (RRS) and maize derived products (Bt11, Bt176, GA21, MON810, T25); qualitative event-specific detection of GM canola (MS8, RF3, GT73), and qualitative event-specific detection of GM maize (NK603, MON863, TC1507, DAS59122). Matrices are raw materials and all solid derived products. GMO analysis is based on the real-time PCR technology and is performed within the regulatory framework conform 18/2001/EC, 1829/2003/EC, 1830/2003/EC and recommendation 787/2004/EC. Within its flexible scope 101 4.8 New hygiene directives in the pilot plant In the context of the legal prescriptions for the production and processing of food, a new auto-control plan, based on the principles of HACCP, was realised. This procedure included the organisation of the plan concerned but also the guarantee of traceability and a range of adaptations of the infrastructure of the pilot plant. A variety of equipment has been installed such as cleaning systems, registration equipment for the most important parameters during the evaporating and spray-drying process, as well as a pest-control system. In a first phase the evaporating and spray-drying parameters are recorded because in this department products used for human consumption are manufactured or transformed. The next phase involves the production of UHT-drinks and ice-cream; finally all production processes will be covered. A further optimisation of the infrastructure of the pilot plant is essential. In cooperation with the Department of Facilitary Management of the Flemish Government many essential renovations will be realised. 4.9 TAD “Hoevezuivel” – Farm dairying in practice Several dairy farmers, dairy farm producers, dairy goat farmers, dairy horse farmers and small and medium enterprises of the dairy industry, can consult since 1 May 2005 the Technological Advisory Service Farm Dairying of ILVO-T&V. This advisory service cooperates with a variety of organisations (IKM Vlaanderen, Boerenbond, Algemeen Boerensyndicaat, KVLV-Agra Steunpunt Hoeveproducten en Belgisch Paardenmelkerijverbond and is financially supported by IWT. The main target of the TAD Farm Dairying is to advise dairy farmers in case of problems or questions about the following subjects: - Milk product quality; - Hygiene and technology in dairy farm production; - Contamination with paratuberculosis. Several forms of advice and sensitisation are used. Examples are the workshop “Tests to detect antibiotic residues on the farm” and lectures about “Critical control points in cheese production and thermometer calibration” and “Food hygiene, auto-control, traceability, notification duty and labelling”. Experts from ILVO-T&V also visit dairy farms to give individual advice. Several problems and questions were resolved by telephone. The nature of the problems concerned antibiotic screening, contaminants, the presence of coliforms and staphylococci, etc. Information about the development of new products such as cheese with red smear bacteria was provided. For the elaboration of analyses and technological experiments the TAD can rely on the laboratories and pilot plant of ILVOT&V-BU. 102 4.10 Agromech The Technological Advisory Service ‘Agromech’ makes recommendations and provides information to farmers, contractors and developers of agricultural machinery around a number of topics: environmental protection, reduction of quality losses of agricultural and horticultural products, optimisation of the efficiency of machines, reduction of the inputs and an improved safety for the operators of machinery. It is obvious that the nature of the questions and the needs of the different target groups are diverse. Farmers and contractors are mainly users of machines. The recommendations to this group are situated especially in the field of adjusting and improving the use of machines: fertiliser spreaders (spreading pattern), potato harvesters (prevention of discolouration), chicory harvesters, sprayers (incl. lance sprayers), etc. The questions from the constructors of agricultural machines are somewhat different. Agromech can assist this group in the design of new machines (e.g. how to guarantee the safety of the user, testing of sprayers, etc.) and with the acquisition of IWT-financing. This kind of service generally necessitates a visit to the farm. After completing the necessary observations a report is made up for the people concerned. Not all recommendations are of this extent. Most questions can be answered with a short recommendation or by providing the necessary information. Two collaborators operate the TAD Agromech and can use the collective expertise of ILVO-T&V-AT. Besides supplying individual service, in 2006 some 120 recommendations, Agromech tries to disseminate as much as possible information to groups. This is done in workshops, demonstrations, publications and through a newsletter. The service is partly free, and this is made possible by the co-financing of the “Begrotingsfonds voor de Grondstoffen” (Fund for Raw Materials). 103 4.12 Laboratory for Spray Application Techniques 4.11 AgriCONSTRUCT The technological advisory service AgriCONSTRUCT started its activities in 2002. Its main target is to advise farmers, contractors and manufacturers of building materials, on the use of building materials in the agricultural sector. IWT approved in 2006 the extension of the project for the next four years. The new project is mainly a continuation of the activities with an expanding of the target groups such as architects and advisory services. Additional attention will be given to find and to support new developments created by the farmers themselves. 480 individual advices were given to farmers, contractors, manufacturers or advisors with 140 farm visits. Besides individual advice, much attention is paid to the quarterly “AgriCONSTRUCT”. Each issue carries a theme analysed in detail by different specialists from the service. Specific advices are grouped in articles, workshops and courses. The technological advisory service publishes also brochures and papers. 104 The Laboratory for Spray Application Techniques is since spring 2002 BELAC-accredited according to ISO 17025 (Certificate 259-T). This accreditation covers four specific tests concerning spray equipment, namely 1) the flow rate of individual nozzles at a given spray pressure, 2) the liquid distribution of an individual nozzle at a given spray height and pressure, 3) the liquid distribution of a set of nozzles mounted on a standard spray boom at a given spray pressure, height and offset angle and 4) the liquid distribution of a field crop sprayer at a given spray pressure, height and offset angle. Apart from these four accredited measuring set-ups, the laboratory possesses a wide range of measuring and test devices to characterise sprayers and/or sprayer parts. The laboratory performs these accredited tests on the one hand to support 4.13 Quality control in connection with the maintenance of milk installations internal research projects with respect to spray application techniques and on the other hand to serve external customers: manufacturers of spray equipment and accessories, scientific institutes, governmental bodies, plant protection companies, etc. In 2006, the laboratory performed assignments of both Belgian and foreign customers. The main assignments resulted from specific requirements set by the guidelines of ‘Good Experimental Practices’ to which customers want/ need to comply. Sprayers to perform spray jobs on experimental plots were delivered to the laboratory to test their liquid distribution, functionality of the safety valve, pressure regulator and pressure gauges. A validation dossier was prepared to obtain a flexible scope for the determination of the liquid distribution of spray booms of up to 3 m using a patternator (with 50 mm wide channels). Validation trials were carried out and summarised in the dossier. Furthermore, flow rate measurements of nozzles were carried out for internal, external and foreign customers. The project group ‘Control’ coordinates all activities in Belgium concerning the maintenance tests of milk installations (milking installations, refrigerated farm milk tanks) and organises the training and support of milk cooling equipment technicians, milking machine technicians and milk yield specialists. The operation of ‘Control’ takes into account the requirements set out by IKM (Integral Quality Monitoring of Milk) and the international standards for milk installations viz. ISO 3918, ISO 5707 and ISO 6690. All measuring and advisory reports of milk installations and refrigerated farm bulk tanks made in 2005 were inventoried during the past year. Subsequently all technicians were evaluated by means of a fully automated evaluation report made in Excel. Therefore, 5% of all reports from each technician, with a minimum of five, were selected at random and checked for accuracy and correctness. Since the start of this project a clear increase in this score has been observed. The measuring equipment of each technician was also checked: pulsator tester, vacuum meter, air flow meter and thermometer. The above results demonstrate the importance of a periodic maintenance of both milk installation and refrigerated farm bulk tanks. Maintenance must be carried out correctly. Hence, providing a qualitative training and regular specialisation courses for technicians is essential. The annual evaluation of service technicians not only serves the goal of supervising their work but must also solve the technical questions and problems that they may encounter with the maintenance of milk installations or milk cooling equipment. Since the start of the project in 1996 a remarkable improvement in the maintenance of milk equipment has been observed. 105 5. Publications 5.1 Articles published in journals and included in the Science Citation Index de la Peña E., Rodríguez-Echeverría S., Van der Putten W. H., Freitas H, Moens M. (2006) Mechanism of control of root-feeding nematodes by arbuscular mycorrhizal fungi in the dune grass Ammophila arenaria. New Phytologist, 169: 829-840. Duquennne B., Eeckhaut T., Werbrouck S., Van Huylenbroeck J. (2006) In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids., Plant Cell Tiss. Organ Cult., DOI 10.1007/s11240-006-9161-8. Animal Sciences Eeckhaut T., Leus L., Van Huylenbroeck J. (2005) Exploitation of flow cytometry for ornamental breeding. Acta Physiologiae Plantarum 27: 743-750. Daeseleire E., Mortier L., Delahaut P., Huyghebaert G. (2006) Determination of concentration levels of anticoccidials in eggs due to the presence of low levels of those compounds in feed for laying hens caused by carryover at the feeding mill. Accred. Qual. Assur. 11: 44-48. Honnay O., Jacquemijn H., Roldan-Ruiz I., Hermy M. (2006) Consequences of prolonged clonal growth on local and regional genetic structure and fruiting success of the forest perennial Maianthemum bifolium, Oikos 112: 21-30. De Campeneere S., De Boever J., De Brabander D.L. (2006) Comparison of rolled, NaOH treated and ensiled wheat grain in dairy cattle diets. Livest. Sci. 99: 267-276. Honnay O., Coart E., Butaye J., Adriaens D., Van Glabeke S., Roldan-Ruiz I. (2006) Low impact of present and historical landscape configuration on the genetics of fragmented Athyllis vulneria populations. Biological Conservation 127: 411-419. De Campeneere S., De Brabander D.L., Vanacker J. (2006) Milk urea concentration as affected by the roughage type offered to dairy cattle. Livest. Sci. 103: 30-39. Fiems L.O., Van Caelenbergh W., De Campeneere S., De Brabander D.L. (2006) Body condition scoring in relation to changes in body weight and performance in Belgian Blue double-muscled beef cows. Anim. Res. 55: 121-130. Lippens M. (2006) Influence of feed protein content on compensatory growth capacity and carcass composition of feed restricted broiler chickens. Zootechnica Int. 1: 34-36. Maertens L., Lebas F., Szendro Zs. (2006) Rabbit milk: a review of quantity, quality and non-dietary affecting factors. World Rabbit Sci. 14: 205-230. Maertens L., Struklec M. (2006) Technical note: Preliminary results with a tannin extract on the performance and mortality of growing rabbits in an enteropathy infected environment. World Rabbit Sci. 14: 189-192. Prunier A., Bonneau M., von Borell E.H., Cinotti S., Gunn M., Fredriksen B., Giersing M., Morton D., Tuyttens F., Velarde A. (2006) A review of the welfare consequences of surgical castration in piglets and the evaluation of non-surgical methods. Animal Welfare 15: 277-289. Szendro Zs, Gyovai M., Maertens L., Biro-Németh E., Radnai I., Matics Zs, Princz Z., Gerencsér Zs., Horn P. (2006) Influence of birth weight and nutrient supply before and after weaning on the performance of rabbit does to age of the first mating. Livest. Sci. 103: 54-64. Van Laar H., Van Straalen W., van Gelder A.H., De Boever J., D’heer B., Vedder H., Kroes R., De Bot P., Van Hees J., Cone J. (2006) Repeatability and reproducibility of an automated gas production technique. Anim. Feed Sci. Technol. 127: 133-150. Vlaeminck B., Fievez V., Tamminga S., Dewhurst R.J., van Vuuren A., De Brabander D.L., Demeyer D. (2006) Milk odd- and branched-chain fatty acids in relation to the rumen fermentation pattern. J. Dairy Sci. 89: 3954-3964. Plant Sciences Ansari M.A., Farman A., Moens M. (2006) Compared virulence of the Belgian isolate of Steinernema glaseri (Rhabditida: Steinernematidae) and the type population of S. scarabaei to white grub species (Coleoptera: Scarabeidae). Nematology 8: 787-791. Ansari M.A., Shah F.A., Tirry L., Moens M. (2006) Field trials against Hoplia philanthus (Coleoptera: Scarabaeidae) with a combination of an entomopathogenic nematode and the fungus Metharizium anisopliae CLO 53. Biological Control 39: 453-459. Ansari M.A., Casteels H., Tirry L., Moens M. (2006) Biology of Hoplia philanthus (Col., Scarabaeidae, Melolonthinae), a new and severe pest in Belgian turf. Environmental Entomology 1500-1507. Jordaens K., De Wolf H., Van Houtte N., Vandecasteele B., Backeljau T. (2006) Genetic variation in two land snails, Cepaea nemoralis and Succinea putris, from sites differing in heavy metal content. Genetica, 128: 227-239. Jordaens K., De Wolf H., Vandecasteele B., Blust R., Backeljau T. (2006) Associations of shell strength, shell morphology and heavy metals in the land snail Cepaea nemoralis (Gastropoda, Helicidae). Science of the Total Environment, 363: 285-293. Kyndt T., Van Droogenbroeck B., Haegeman A., Roldan-Ruiz I., Gheysen G. (2006) Cross-species microsatellite amplification in Vasconcellea and rlated genera an d their use in germplasm classification.,Genome 49: 786-798. Łączyński A., De Moor A., Dierickx W., Moens M., Darius P., Sonck B., Ramon H.(2006) The effect of hydraulic agitation on the viability of the nematode Heterorhabditis bacteriophora. Crop Protection 25: 1135-1141. Leus L., Dewitte A., Van Huylenbroeck J., Vanhoute N., Van Bockstaele E., Höfte M. (2006) Podosphera pannosa (syn. Spaerotheca pannosa) on Rosa and Prumus spp.: characterisation of pathotypes by differential plant reactions and ITS sequences. , Journal of Phytopathology, 154: 23-28. Mestdagh I., Lootens P., Van Cleemput O. & Carlier L. (2006) Variation in organic-carbon concentration and bulk density in Flemish grassland soils. Journal of Plant Nutrition and Soil Science, 169(5): 616-622. Moerkerke B., Goetghebeur E., De Riek J., Roldan-Ruiz I. (2006) Significance and impotence: towards a balanced view of the null and the alternative in marker selection for plant breeding. Journal of the Royal Statistical Society Series A, J.R. Statist.Soc. A.(2006)169: Part 61-79. Nybom H., Esselinck G.D., Werlemark G., Leus L., Vosman B. (2006) Unique genomic configuration revealed by microsatellite DNA in polyploid dogroses, Rosa sect. Caninae. Journal of Evolutionary Biology 19: 635-648. Phan Ke L., Spiridonov S.E., Subbotin S.A., Moens M. (2006) Four new species of Steinernema Travassos, 1928 with short infective juveniles from Vietnam. Russian Journal of Nematology 14: 11-29. Pudasaini M., Viaene N., Moens M. (2006) Effect of marigold (Tagetes patula) on population dynamics of Pratylenchus penetrans in a field. Nematology 8: 477-484. Pudasaini M.P., Schomaker C.H., Been T.H., Moens M. (2006) The vertical distribution of the plant-parasitic nematode, Pratylenchus penetrans, under four field crops. Phytopathology 96: 226-233. Vandecasteele B., Buysse C.A., Tack F.M.G. (2006) Metal uptake in maize, willows and poplars on impoldered and freshwater tidal marshes in the Scheldt estuary. Soil Use and Management, 22: 52-61. Chaves B., De Neve S., Boeckx P., Berko C., Van Cleemput O., Hofman G. (2006) Manipulating the N release from 15N labelled celery residues by using straw and vinasses. Soil Biology and Biochemistry, 38: 2244-2254. Van der Putten W. H., Raeymakers A., Piskiewicz A., Kerry B.R., Davies K.G., Costa S., Cook R., HolG., Moens M., de la Peña E., Mateille T., Fargette M., Maher N., Van der Wurff A.W.G., Freitas H., Rodríguez-Echeverría S. (2006) Nematode interactions in nature: models for sustainable control of nematode pests of crop plants? Advances in Agronomy, 89: 277-295. Chen S., Xiuhua L., Yan A., Spriridonov S.E., Moens M. (2006) A new entomopathogenic nematode, Steinernema hebeiense sp. n. (Rhabditida: Steinernematidae), from North China. Nematology 8: 563-574. Wesemael W., Perry R., Moens M. (2006) The influence of root diffusate and host age on hatching of the root-knot nematodes, Meloidogyne chitwoodi and M. fallax. Nematology 8 (6): 895-902. Coart E., Van Glabeke S., De Loose M., Larsen A.S., Roldan-Ruiz I. (2006) Chloroplast diversity in the genus Malus: new insights into the relationship between the European wild apple (Malus sylvestris(L.) Mill.) and the domesticated apple (Malus domestica Borkh.), Molecular Ecology 15: 2171-2182. De Keukeleire J., Roldan-Ruiz I., Van Bockstaele E., Heyerick A., De Keukeleire D. (2006) Efficient Extraction of High-Quality total RNA from various Hop Tissues (Humulus lupulus L.), Preparative Biochemistry & Biotechnology 36: 355362. 106 de la Peña E., Moens M., Van Aelst A., Karssen G. (2006) Description of Pratylenchus dunensis sp. n. (Nematoda: Pratylenchidae), a parasite of the dune grass Ammophila arenaria L. Link. Nematology, 1: 79-88. Technology & Food Bohez L., Ducatelle R., Pasmans F., Botteldoorn N., Haesebrouck F., Van Immerseel F. (2006) Salmonella enterica serovar Enteritidis colonization of the chicken caecum requires the HilA regulatory protein. Vet. Microbiol., 166: 220210. Botteldoorn N., Van Coillie E., Grijspeerdt K., Werbrouck H., Haesebrouck F., Donne E., D’haese E., Heyndrickx M., Pasmans F., Herman L. (2006) Real-time reverse transcription PCR for the quantification of the mntH expression of Salmonella enterica as a function of growth phase and phagosome-like conditions. J. Microbiol Methods., 66: 125-135. Werbrouck H., Grijspeerdt K., Botteldoorn N., Van Pamel E., Rijpens N., Van Damme J., Uyttendaele M., Herman L., Van Coillie E. (2006) Differential inlA and inlB expression and interaction with human intestinal and liver cells by Listeria monocytogenes strains of different origins. Appl. Environ. Microbiol., 72: 3862-3871. Daeseleire E., Mortier L., Delahaut P., Huyghebaert G. (2006) Determination of concentration levels of anticoccidials in eggs due to the presence of low levels of those compounds in feed for laying hens caused by carryover at the feeding mill. Accreditation and Quality Assurance, 11: 44-48. 5.2 Other scientific articles De Graaf D.C., De Vos P., Heyndrickx M., Van Trappen S., Peiren N., Jacobs F.J. (2006) Identification of Paenibacillus larvae to the subspecies level: an obstacle for AFB diagnosis. J. Invertebr. Pathol., 91: 115-123. De Reu K., Grijspeerdt K., Herman L., Heyndrickx M., Uyttendaele M., Debevere J., Putirulan F.F., Bolder N.M. (2006) The effect of a commercial UV disinfection system on the bacterial load of shell eggs. Lett. Appl. Microbiol., 42: 144-148. De Reu K., Grijspeerdt K., Heyndrickx M., Messens W., Uyttendaele M., Debevere J., Herman L. (2006) Influence of eggshell condensation on eggshell penetration and whole egg contamination with Salmonella enterica serovar Enteritidis. J. Food Prot., 69: 1539-1545. De Reu K., Grijspeerdt K., Heyndrickx M., Uyttendaele M., Debevere J., Herman L. (2006) Bacterial shell contamination in the egg collection chains of different housing systems for laying hens. Brit. Poultry Sci., 47: 163-172. De Reu K., Grijspeerdt K., Messens W., Heyndrickx M., Uyttendaele M., Debevere J., Herman L. (2006) Eggshell factors influencing eggshell penetration and whole egg contamination by different bacteria, including Salmonella enteritidis. Int. J. Food Microbiol., 112: 253-260. De Schijver A., Devos Y., Van Den Bulcke M., Cadot P., De Loose M., Riheul D., Sneyers M. (2006) Risk assessment of GM stacked events obtained from crosses between GM events. Trends Food Sci. Technol., published online: DOI 10.1016/j.tifs.2006.09.002. Gholamiandekhordi A., Ducatelle, R., Heyndrickx M., Haesebrouck F., Van Immerseel F. (2006) Molecular and phenotypical characterization of Clostridium perfringens isolates from poultry flocks with different disease status. Vet. Microbiol., 113: 143-52. Holst-Jensen A., De Loose M., Van Den Eede G. (2006) Coherence between the legal requirements and approaches for detection of genetically modified organisms (GMOs) and their derived products. J. Agric. Food Chem., 54: 27992809. Klancnik A., Botteldoorn N., Herman L., Smole S. (2006) Survival and stress induced expression of groEL and rpoD of. Campylobacter jejuni from different growth phases. Int. J. Food Microbiol., 112: 200-207. Messens W., Grijspeerdt K., Herman L. (2006) Eggshell penetration of hen’s eggs by Salmonella enterica serovar Enteritidis upon various storage conditions. Brit. Poultry Sci., 47: 554-560. Paoletti C., Heissenberger A., Mazzara M., Larcher S., Grazioli E., Corbisier P., Hess N., Berben G., Lubeck P., De Loose M., Moran G., Henry C., Brera C., Folch I., Ovesna J., Van Den Eede G. (2006) Kernel Lot Distribution Assessment (KeLDA): a study on the distribution of GMO in large soybean shipments. Eur. Food Res. Technol., 224: 129-139. Animal Sciences Hostens K., Moulaert I. (2006) De epi-, macro- en visfauna op de Vlakte van de Raan. In: Coosen J., Mees J., Seys J., Fockedey N. (eds.). Studiedag: De Vlakte van de Raan van onder het stof gehaald, Oostende (B). VLIZ special Publication, 35: 116-135. Milà i Canals L., Bauer C., Depestele J., Dubreuil A., Freiermuth R., Gaillard G., Michelsen O., Müller-Wenk R., Rydgren B. (2006) Key elements in a framework for land use impact assessment in LCA. Version 2, 21/03/06. Discussion paper for the Land Use sub-taskforce within the UNEP/SETAC Life Cycle Initiative Programme on LCIA, Task-Force 2 on natural resources and land use. Available from http://www.lci-network.de/cms/content/pid/591. Vanhonacker F., Van Poucke E., Nijs G., Braeckman J., Tuyttens F., Verbeke W. (2006) Defining animal welfare from a citizen and consumer perspective: exploratory findings from Belgium. Ethics and the Politics of Food. Wageningen Acad. Publ. (NL): 580-582. Van Poucke E., Vanhonacker F., Nijs G., Braeckman J., Verbeke W., Tuyttens F. (2006) Defining the concept of animal welfare: integrating the opinion of citizens and other stakeholders. Ethics and the Politics of Food. Wageningen Acad. Publ. (NL): 555-559. Plant Sciences Barrière Y., Alber D., Dolstra O., Lapierre C., Motto M., Ordas A., Van Waes J., Vlasminkel L., Welcker C., Monod J.P. (2006) Past and prospects of forage maize breeding in Europe. I. The grass cell wall as a basic of genetic variation and future improvements in feeding value. Maydica 50: 259-274. Chaves B., De Neve S., Hofman G., Van Cleemput O. (2006) Conserving N from high N crop residues under field conditions by using on- and off-farm organic waste products. Acta Horticulturae, 700: 249-254 Dewitte A., Eeckhaut T., Van Huylenbroeck J., Van Bockstaele E. (2006) Flow cytometric detection of unreduced gametes in Begonia, Acta Horticuturae 714:107-112. Dewitte A., Eeckhaut T., Van Huylenbroeck J., Van Bockstaele E. (2006) Application of unreduced gametes in ornamentals. , Acta Horticulturae 714: 107112. Eeckhaut T., Van Huylenbroeck J., De Schepper S., Van Labeke M-C. (2006) Breeding for Polyploidy in Belgian Azalea (Rhododendron simsii hybrids). , Acta Horticulturae 714: 113-118. Van Laere K., Van Huylenbroeck J., Van Bockstaele E. (2006) Breeding strategies to increase genetic variability in Hibiscus syriacus. , Acta Horticulturae 715: 75-81. Papazova N., Taverniers I., Degrieck I., Van Bockstaele E., Joost H., De Loose M. (2006) Real Time PCR quantification of T25 maize seeds – influence of the genetic structures in the maize kernel on the quantitative analysis. Seed Sci. Technol., 34: 321-331. Van Waes J. (2006) Harmonization of VCU-testing methods for maize varieties in a European context. Acta Agronomica Hungarica 54(3): 365-377. Papazova N., Windels P., Depicker A., Taverniers I., Roldan-Ruiz I., Milcamps A., Van Bockstaele E., Van Den Eede G., De Loose M. (2006) Sequence stability of the T-DNA/plant junctions in tissue culture in Arabidopsis transgenic lines. Plant Cell Rep., 25: 1362-1368. De Reu K., Grijspeerdt K., Heyndrickx M., Uyttendaele M., Debevere J., Herman L. (2006) Bacterial shell contamination in the egg handling chains of different housing systems for laying hens. World’s Poult. Sci. J., 62 (Suppl.): 562-563. Rajkovic A., Uyttendaele M., Courtens T., Heyndrickx M., Debevere J. (2006) Prevalence and characterisation of Bacillus cereus in vacuum packed potato puree. Int. J. Food Sci. Technol., 41: 878-884. Technology & Food De Reu K., Van Coillie E., Grijspeerdt K., Heyndrickx M., Rodenburg B., Tuyttens F., Zoons J., Herman L. (2006) Microbiological survey of furnished cages and non-cage systems for laying hens. World’s Poult. Sci. J., 62 (Suppl.): p.563. Scheldeman P., Herman L., Foster S., Heyndrickx M. (2006) Bacillus sporothermodurans and other highly heat-resistant spore formers in milk. J. Appl. Microbiol., 101: 542-555. De Reu K., Heyndrickx M., Grijspeerdt K., Rodenburg B., Tuyttens F., Uyttendaele M., Debevere J., Herman L. (2006) Assessment of the vertical and horizontal aerobic bacterial infection of shell eggs. World’s Poult. Sci. J., 62 (Suppl.): p.564. Smet K., Raes K., De Smet S. (2006) Novel approaches in measuring the antioxidative potential of animal feeds: the FRAP and DPPH methods. J. Sci. Food and Agricult., 86: 2412-2416. De Ruyck H., De Ridder H. (2006) Determination of albendazol sulphoxide and netobimin in cow’s milk by liquid chromatography and tandem mass spectrometric detection. Milchwissenschaft, 61 (1): 72-75. Stuyt L.C.P.M., Dierickx W. (2006) Design and performance of materials for subsurface drainage systems in agriculture. Agric. Water Management, 86: 5059. De Zutter L., Heyndrickx M., Herman L., Posse B. (2006) Selective isolation media for Shigatoxin producing Escherichia coli (STEC). Invention disclosure form, Technologie Transfer Ugent: 18 p. Van Canneyt T., Dierickx W., Verschoore R., Ramon H., Sonck B. (2006) Effect of preload, vibration frequency, temperature and specific gravity of potato tissue on visco-elastic vibration damping and complex modulus properties. Biosystems Engineering, 94: 415-427. Messens W., Goris J., Heyndrickx M., Herman L. (2006) Epidemiological study of Campylobacter contamination of poultry farms in Belgium by amplified fragment length polymorphism analysis. World’s Poult. Sci. J., 62 (Suppl.): 558559. 107 Nuyttens D., De Schampheleire M., Baetens K., Sonck B. (2006) PDPA LaserBased Characterisation of Agricultural Spray Nozzles. Agric. Eng. Int.: the CIGR E-journal. Manuscript PM 06 024. Vol. VIII. December. Uyttendaele M., Herman L., Daube G., Lauwers S., Vandeoplas Y., Saegerman C., Debevere J. (2006) Risico’s verbonden aan Enterobacter sakazakii in zuigelingenvoeding. Tijdschrift voor Geneeskunde, 62: 21-27. 5.3 Books and chapters in books Animal Sciences Bekaert K. (2006) Development of a Quality Index Method scheme to evaluate freshness of tub gurnard (Chelidonichthys lucernus). In: Luten J.B., Jacobsen C., Bekaert K., Saebo A., Oehlenschlaeger J. (eds). Seafood research from fish to dish. Quality, safety and processing of wild and farmed fish. Wageningen, Wageningen Academic Publishers: 289-296. Van Gijseghem D., Van Hoof K., Wustenberghs H., D’hooghe J., Fernagut B., Eppinger R., De Simpelaere K., Overloop S. (2006) Vermesting. In: Van Steerteghem M. (ed.). Milieurapport Vlaanderen, MIRA-T 2006, Focusrapport. Vlaamse Milieumaatschappij, Mechelen en LannooCampus, Leuven. Van Passel S., Lauwers L., Van Huylenbroeck G. (2006) Factors of farm performance: an empirical analysis of structural and managerial characteristics. In: Mann, S. (ed.), Causes and impacts of agricultural structures, Nova Science Publishers, Inc., New York: 3-22. Plant Sciences Carlier L. et al. (2006) Manualul sistemilor de producţie ecologică (Risoprint Cluj-Napoca): 114p. Carlier L.et al. (2006) HAPЪЧHИK эa opгaнuчнo зeмegeлue (Diagnosis Press, Sofia): 120 p. Duncan L.A., Moens M. (2006) Migratory endoparasitic nematodes. In: Plant Nematology. Perry, R.N. & M. Moens (Eds). CABI Publishing, Wallingford, UK: 123-152. Bell M., Redant F., Tuck I. (2006) Chapter 13: Nephrops species. In: B.F. Phillips (ed.). Lobsters: Biology, Management, Aquaculture and Fisheries. Blackwell Publishing Limited, Oxford: 412-461. Eeckhout T., Van Laere K., De Riek J., Van Huylenbroeck J. (2006) Overcoming Interspecific Barriers in Ornamental Plant Breeding. Global Science Books Flariculture, Ornamental and Plant Biotechnology volume 1: 540-551. Degraer S., Wittoeck J., Appeltans W., Cooreman K., Deprez T., Hillewaert H., Hostens K., Mees J., Vanden Berghe W., Vincx M. (2006) De macrobenthosatlas van het Belgisch deel van de Noordzee. Federaal Wetenschapsbeleid D/2005/1191/5: 164 p. Karssen G., Moens M. (2006) Root-knot nematodes. In: Plant Nematology. Perry, R.N. & M. Moens (Eds). CABI Publishing, Wallingford, UK: 59-90. Degraer S., Wittoeck J., Appeltans W., Cooreman K., Deprez T., Hillewaert H., Hostens K., Mees J., Vanden Berghe W., Vincx M. (2006) The macrobenthos atlas of the Belgian part of the North Sea. Belgian Science Policy D/2005/1191/3 : 164 p. Degraer S., Wittoeck J., Appeltans W., Cooreman K., Deprez T., Hillewaert H., Hostens K., Mees J., Vanden Berghe W., Vincx M. (2006) L’ atlas du macrobenthos de la partie Belge de la Mer du Nord. Politique Scientifique Fédérale D/2005/1191/4 : 164 p. Demaré W. (2006) Chapter 11: Fisheries-Based Management and Advice in Europe. In: Motos L. and Wilson D.C. (eds.). The Knowledge Base for Fisheries Management. Developments in Aquaculture and Fisheries Science, Vol. 36. Elsevier, Amsterdam: 145-160. Luten J.B., Jacobsen C., Bekaert K., Saebo A., Oehlenschlaeger J. (eds) (2006) Seafood research from fish to dish. Quality, safety and processing of wild and farmed fish. Wageningen: Wageningen Academic Publishers: 567 p. Raemaekers M., Derveaux S., Parmentier K. (2006) Polychlorinated biphenyls and organochlor pesticides in brown shrimp (Crangon crangon) of the Belgian Continental Shelf. In: Luten J.B., Jacobsen C., Bekaert K., Saebo A., Oehlenschlaeger J. (eds). Seafood research from fish to dish. Quality, safety and processing of wild and farmed fish. Wageningen: Wageningen Academic Publishers: 489-496. Struelens E., Tuyttens F., Van Nuffel A., Zoons J., Janssens A., Cox M., De Baere K., Leroy T., Vranken E., Berckmans D., Buyse J., Sonck B. (2006) Huisvesting van leghennen in verrijkte kooien: preferentie voor legnest- en zitstokdesign, invloed op welzijn en gezondheid, geautomatiseerde gedragsanalyse. Federale Overheidsdienst Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu: 158 p. Van Poucke E., Van Nuffel A., Van Dongen S., Huyghebaert G., De Brabander D., Sonck B., Lens L., Tuyttens F. (2006) Fluctuerende asymmetrie bij vleeskippen als een indicator van welzijn. Federale Overheidsdienst Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu: 132 p. Viaene N., Kerry B., Coyne D. (2006) Biological and cultural management. In: Plant Nematology, Perry R. en Moens M., eds. 346-369. Social Sciences Henry de Frahan B., Buysse J., Polomé P., Fernagut B., Harmignie O., Lauwers L., Van Huylenbroeck G., Van Meensel J. (2006) Positive Mathematical Programming for Agricultural and Environmental Policy Analysis: Review and Practice. In: Weintraub A., Bjorndal V, Epstein R., Romero C. (Eds.) Management of Natural Resources: A Handbook of Operations Research Models, Algorithms and Implementations. Kluwer´s International Series in Operations Research and Management Science, Frederick S. Hillier, Series Editor. Kluwer Academic Publishers. Overloop S., Bossuyt M., Buysse M., Ducheyne S., Dumortier M., Eppinger R., Genouw G., Stemgée K., Van Gijseghem D., Van Hoof K., Vogels N., Wustenberghs H., D’hooghe J., Fernagut B. (2006) Milieurapport Vlaanderen MIRA, Achtergronddocument, Thema Vermesting. http://www.milieurapport.be. Overloop S., Wustenberghs H., Hens M. (2006) Landbouw & milieu. In: Platteau J. (ed.) Landbouwrapport 2005. Vlaamse overheid, Departement Landbouw en Visserij: 195-218. Perry R.N. & Moens M. (Eds) (2006) Plant Nematology. CABI Publishing, Wallingford, UK: 432 p. Subbotin S.A., Moens M. (2006) Molecular taxonomy and phylogeny. In: Plant Nematology. Perry, R.N. & M. Moens (Eds). CABI Publishing, Wallingford, UK: 34-58. Technology & Food Dierickx W. (2006) Soil Physics. Land Use and Land Cover, from Encyclopedia of Life Support Systems (EOLSS), Developed under the Auspices of the UNESCO, Eolss Publishers, Oxford, UK [http://www.eolss.net]. 5.4 Proceedings and abstracts of symposia and congresses Animal Sciences Arnouts S., Lippens M. (2006) The effect of globin, a water-soluble emulsifier, on broiler performance. 12th Eur. Poultry Conf., Verona (IT): 4 p. Bekaert K., Derveaux S. (2006) Development of a quality index method scheme to evaluate freshness of tub gurnard (Chelidonichthys lucernus). In: Mees, J., Seys, J. (eds.) (2006). VLIZ Young Scientists’ Day, Brugge (B) book of abstracts. VLIZ Special Publication, 30: p. 23. Byrne D., Nijs G., Millet S., Van Oeckel M.J., Warnants N., Sonck B., Vestergaard J., Haugen J.-E., Verheyden K., De Brabander H., Tuyttens F., De Brabander D.L. (2006) Early and reliable detection of boar taint and its genetic predisposition. Proc. 52th Int. Cong. Meat Science and Technology, Dublin (IE): 73-74. De Boever J., Vanacker J., De Brabander D.L. (2006) Estimation of the protein value of roughages and concentrates: solubility versus washability. Proc. 31ste Studiedag NVO, Blijdorp (NL): 70-71. De Campeneere S., De Boever J., De Brabander D.L. (2006) Comparison of ensiled grass-white clover and grass-red clover mixture for dairy cattle. In: Strandberg E., Moreira O., Fourichon C., Vestergaard M., Lazzaroni C., Gauly M., Wenk C., Martin-Rosset W., Hermansen J., Thomas C., Book of abstracts 57th Ann. Meet. EAAP, Antalya (TR): p. 117. De Campeneere S., De Brabander D.L., Vanacker J. (2006) Evaluation of rolled wheat and CCM as concentrate replacers for dairy cattle. 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(2006) Breeding strategies to increase genetic variability in Hibiscus syriacus, Eucarpia 22th Int. Symp. Section ornamentals: ‘Breeding for Beauty’, San Remo (I). Van Waes C. (2006) Forage quality: from table values until on-line analyses at harvest. Proc. final seminar ‘optimising farm grown legumes in a crop rotation system for cattle feeding in a sustainable agriculture’, Ozolnieku (LT), 12/10/2006: 11p. Viaene N., Eloot B., De Vis R., Decadt R., Vergote N., Bleyaert P., Peeters L., Trybou M., Heungens K. (2006) Critical use of MeBr for soil disinfestation in Belgium during 2005. Proc. 58th Int. Symp. on Crop Prot., Gent: p. 122. Viaene N., Cornelissen K. (2006) Biofumigation as a management tool for Meloidogyne spp. in the glasshouse, a case study. Proc. 58th Int. Symp. on Crop Prot., Gent: p. 207. Viaene N., Moens M. (2006) Host plant status of cover crops for Pratylenchus crenatus. Prog. and Abstr. 28th Int. Symp. ESN, Blagoevgrad (BUL): p. 125. Vidican R., Rotar I., Carlier L. (2006) Perspectives of the organic farming in Romania. Bulitenul, Seria Agricultura, UASVM, Cluj-Napoca (R), 5-6/10/2006, 62: 198-200. Botteldoorn N., Van Coillie E., Heyndrickx M., Haesebrouck F., Pasmans F., Herman L. (2006) Quantification of the mntH gene expression of Salmonella enterica as a function of growth phase and phagosome-like conditions by realtime RT-PCR. Proc. Int. Symp. Salmonella and Salmonellosis, St. Malo (F): 307308. Boyen F., Pasmans F., Botteldoorn N., Adriaensen C., Hernalsteens J.P., Ducatelle R., Van Immerseel F., Heyndrickx M., Haesebrouck F. (2006) A Salmonella Typhimurium ssrA deletion mutant is not impaired in the colonization of pigs. Proc. Int. Symp. Salmonella and Salmonellosis, St. Malo (F): 259-262. Boyen F., Pasmans F., Donné E., Botteldoorn N., Heyndrickx M., Van Immerseel F., Herman L., Decostere A., Ducatelle R., Haesebrouck F. (2006) Role of the mntH gene in the pathogenesis of Salmonella Typhimurium infections in pigs Proc. Int. Symp. Salmonella and Salmonellosis. Proc. Int. Symp. Salmonella and Salmonellosis, St. Malo (F): 305-306. Braekman A., Coudijzer K., De Block J. (2006) Study of the residual proteolytic activity in milk powders of different heat classes. Book of abstracts CIFST/AAFC Joint Conf.: At the forefront of agri-food innovations, Montreal (CAN): p.186. Daeseleire E., Mortier L., Delahaut P., Huet A.C., Huyghebaert G., Van Peteghem C. (2006) Integrated approach for the control on residues of coccidiostats in eggs: results of animal experiments and monitoring of commercially available eggs, Book of abstracts 5th Int. Symp. on Hormone and Veterinary Drug Residues Analysis, Antwerpen (B): p.7. Daeseleire E., Reybroeck. W. (2006) Determination of sulpha drugs in honey by LC-MS/MS. Book of abstracts 5th Int. Symp. on Hormone and Veterinary Drug Residues Analysis, Antwerpen (B): p.79. Daeseleire E., Reybroeck W. (2006) Determination of sulpha drugs in honey by LC-MS/MS. Proc. 2sd Eur. Conf. of Apidology EurBee, Praag (CZ): 118-119. Daeseleire E., Reybroeck W. (2006) Determination of sulpha drugs in honey by LC-MS/MS. Book of abstracts CIFST/AAFC Joint Conf.: At the forefront of agrifood innovations, Montreal (CAN): p.185. Datukishvili N., Zaalishvili T., Taverniers I., De Loose M. (2006) Development and validation of DNA-based methods for GM food analysis. INTAS Workshop – South-Caucasus 2006, Scientific Cooperation and Collaborative Call, Tbilisi, Georgia: 2 p. De Reu K., Grijspeerdt K., Heyndrickx M., Herman L., Uyttendaele M., Debevere J., Putirulan F.F., Bolder N.M. (2006) The effect of UV irradiation on the bacterial load of shell eggs. Book of abstracts CIFST/AAFC Joint Conf.: At the forefront of agri-food innovations, Montreal (CAN): p.180. De Reu K., Grijspeerdt K., Heyndrickx M., Messens W., Uyttendaele M., Debevere J. , Herman L. (2006) Influence of eggshell condensation on the bacterial eggshell penetration and the whole egg contamination with Salmonella enterica serovar Enteritidis. Book of abstracts 20th Int. ICFMH Symp., Bologna (I): p.208. De Reu K., Grijspeerdt K., Heyndrickx M., Uyttendaele M., Debevere J., Herman L. (2006) Bacterial shell contamination in the egg handling chains of different housing systems for laying hens. Proc. 12th Eur. Poultry Conf., Verona (I): 6 p. Wesemael W., Moens M. (2006) Vertical distribution of Meloidogyne chitwoodi under different field crops and black fallow. Prog. and Abstr. 28th Int. Symp. ESN, Blagoevgrad (BUL), p. 73. De Reu K., Van Coillie E., Grijspeerdt K., Heyndrickx M., Rodenburg B., Tuyttens F., Zoons J., Herman L. (2006) Microbiological survey of furnished cages and non-cage systems for laying hens. Proc. 12th Eur. Poultry Conf., Verona (I): 6 p. Zwart R.S., Muylle H., Roldán-Ruiz I., Van Bockstaele E., Van Huylenbroeck J. (2006) Genetic diversity of Fusarium Head Blight QTLs in Western European Wheat. Eucarpia – Cereals Section Meeting. Lleida (SP). De Ruyck H., De Ridder H. (2006) Optimisation and validation of a liquid chromatographic-electrospray tandem mass spectrometric multiresidue method for tetracyclines in cow’s milk. Book of abstracts 5th Int. Symp. on Hormone and Veterinary Drug Residue Analysis, Antwerpen (B): p.84. Zwart R.S., Muylle H., De Loose M., Roldán-Ruiz I. (2006) Genetic diversity of Fusarium Head Blight QTLs among Winter Wheat cultivars and Breeding lines. , Plant & Animal Genomes XIV, San Diego (California, USA). Technology & Food De Ruyck H., De Ridder H. (2006) Optimisation and validation of a liquid chromatographic-electrospray tandem mass spectrometric multiresidue method for tetracyclines in cow’s milk. Book of abstracts CIFST/AAFC Joint Conf.: At the forefront of agri-food innovations, Montreal (CAN): p.184. Baert L., Wobus C.E., Van Coillie E., Thackray L.B., Virgin H.W., Debevere J., Uyttendaele M. (2006) Heat inactivation of Murine Norovirus 1, a surrogate for human noroviruses – Correlation between detection of viral RNA with a novel real-time RT-PCR and infectivity. Symp. Eigen Werk, Wageningen (NL): 1 p. De Schampheleire M., Nuyttens D., Steurbaut W., Baetens K., Verboven P., Nicolaï B., Ramon H., Sonck B. (2006) The assessment of particle spray drift damage for ten major crops in Belgium. Asp. of Appl. Biol., 77(1), Robinson College, Cambridge (GB): 275-280. Baetens K., Nuyttens D., Verboven P., De Schampheleire M., Nicolaï B., Sonck B., Steurbaut W., Ramon H. (2006) The relative importance of environmental and field sprayer parameters for reducing drift: a CFD sensitivity study. Asp. of Appl. Biol., 77(2), Cambridge (GB): 303-311. De Schampheleire M., Spanoghe P., Cornelis W., Nuyttens D., Baetens K. (2006) Classification of spray nozzles based on droplet size distributions and wind tunnel tests. Comm. in Agric. and Appl. Biol. Sciences, Gent (B). Botteldoorn N., Van Coillie E., Goris J., Scheldeman P., Herman L., Heyndrickx M. (2006) The unique association of Salmonella Enteritidis with eggs and the diversity within Salmonella Enteritidis. Proc. Int. Symp. Salmonella and Salmonellosis, St. Malo (F): 373-374. Botteldoorn N., Van Coillie E., Goris J., Scheldeman P., Herman L., Heyndrickx M. (2006) The unique association of Salmonella Enteritidis with eggs and the diversity within Salmonella Enteritidis. Book of abstracts 11th Conf. on Food Microbiology, Gent (B): p.132. De Schampheleire M., Spanoghe P., Steurbaut W., Nuyttens D., Baetens K., Cornelis W., Gabriels D. (2006) The efficiency of drift-reducing measures to protect the Flemish environment. 11th IUPAC Inter. Cong. of Pesticide Chemistry, Kobe (JAP). Donaghy J.A., Rowe M.T., Rademaker J.L.W., Hammer P., Herman L., De Jonghe V., Blanchard B., Vindel E. (2006) An inter-laboratory EU ringtrial for the detection and isolation of Mycobacterium avium subsp. paratuberculosis from faecally contaminated milk. Venomy workshop (Laboratory diagnosis of Mycobacteria), Jena (D): 1 p. 111 Franck A., Sonck B., De Belie N. (2006) Frictional Properties between Bovine claw and Concrete Floor. Book of Abstracts 16th CIGR World cong., AgEng, 64th VDI MEG Inter. Conf. Agricultural Engineering: Agricultural Engineering for a Better World. Bonn (D), 471-472. Ghedira R., Papazova N., Taverniers I., Depicker A., Van Bockstaele E., Bartegi A., De Loose M. (2006) Stabilité et Quantification des OGM. Proc. Les XVIemes Journées Nationales de Biologie “Gestion des Bioressources, Hammamet (TUN), Tunesië : p.54. Goris J., Dierick N., Herman L., Heyndrickx M. (2006) Inhibition of Salmonella Typhimurium by medium chain fatty acids in an in-vitro simulation of the pig intestinal microbial ecosystem. Proc. Int. Symp. Salmonella and Salmonellosis, St. Malo (F): 539-542. Heyndrickx M. (2006) Multiple typing for the epidemiological study of the contamination of broilers with Salmonella from the hatchery to the slaughterhouse. Int. Satellite Cong. Platform for Scientific Concertation: Food safety, Antwerpen (B): 8 p. Huet A.-C., Mortier L., Daeseleire E., Fodey T., Elliott C., Delahaut P. (2006) Development of an ELISA screening test for halofuginone, nicarbazin and nitroimidazoles in egg and chicken muscle. Book of abstracts 5th Int. Symp. on Hormone and Veterinary Drug Residues Analysis, Antwerpen (B): p.124. Messens W., Goris J., Heyndrickx M., Herman L. (2006) Epidemiological study of Campylobacter contamination of poultry farms in Belgium by amplified fragment length polymorphism analysis. Proc. 12th Eur. Poultry Conf., Verona (I): 6 p. Messens W., De Reu K., Grijspeerdt K., Herman L. (2006) Factors influencing eggshell penetration and whole egg contamination with Salmonella Enteritidis. Book of abstracts CIFST/AAFC Joint Conf.: At the forefront of agri-food innovations, Montreal (CAN): 138-139. Missotten J., Michiels J., Goris J., Herman L., Heyndrickx M., De Smet S., Dierick N.A. (2006) Fermented liquid feed for weaning piglets. Proc. 31th NVO Meeting, Rotterdam (NL): 16-17. Missotten J., Michiels J., Goris J., Herman L., Heyndrickx M., De Smet S., Dierick, N. (2006) Fermented liquid feed for weaned piglets. Proc. 12th PhD Symp. Appl. Biol. Sci., Gent (B): 227-230. Missotten J., Michiels J., Goris J., Herman L., Heyndrickx M., De Smet S., Dierick N. (2006) Screening of 2 probiotic products for use in fermented liquid feed. Proc. 10th Int. Symp. on Digestive Physiology in Pigs, Vejle (DK): p.32. Nuyttens D., Baetens K., Nicolaï B., Ramon H., De Schampheleire M., Steurbaut W., Sonck B. (2006) The effect of droplet characteristics on spray drift. Proc. Agricultural Engineering World Cong., Bonn (D), paper No. 859. Nuyttens D., De Schampheleire M., Steurbaut W., Baetens K., Verboven P., Nicolaï B., Ramon H., Sonck B. (2006) Characterization of agricultural sprays using laser techniques. Asp. of Appl. Biol., 77(1), Cambridge (GB):179-188. Nuyttens D., De Schampheleire M., Steurbaut W., Baetens K., Verboven P., Nicolaï B., Ramon H., Sonck B. (2006) Experimental study of factors influencing the risk of drift from field sprayers, Part 1: Meteorological conditions. Asp. of Appl. Biol., Cambridge (GB), 77(2): 321-329. Nuyttens D., De Schampheleire M., Steurbaut W., Baetens K., Verboven P., Nicolaï B., Ramon H., Sonck B. (2006) Experimental study of factors influencing the risk of drift from field sprayers, Part 2: Spray application technique. Asp. of Appl. Biol., 77(2), Cambridge (GB): 331-339. Papazova N. (2006) Applications of Conformation Sensitive Capillary Electrophoresis (CSCE) in the GMO research. International Satellite Conference, Platform for Scientific Concertation: Food safety, Antwerpen, (B): 5 p. Papazova N., Windels P., Depicker A., Taverniers I., Roldan-Ruiz I., Milcamps A., Van Bockstaele E., Van Den Eede G., De Loose M. (2006) Sequence stability of T-DNA/plant junctions in tissue culture in Arabidopsis transgenic lines. 20th Anniversary meeting of the Belgian Plant Tissue Culture group “Tissue culture – facing the future”, Gembloux (B): p.13. Reybroeck W., Ooghe S. (2006) Validation of the TwinSensor Milk for rapid screening of b-lactams and tetracyclines in milk, Book of abstracts 5th Int. Symp. on Hormone and Veterinary Drug Residue Analysis, Antwerpen (B): p. 26. Reybroeck W., Ooghe S., Daeseleire E. (2006) Presence of antibiotics and sulfonamides in honey and royal jelly on the European market. Proc. 2nd Eur. Conf. of Apidology EurBee, Praag (CZ): p.117. Rodenburg T.B., Tuyttens F.A.M., De Reu K., Herman L., Zoons J., Sonck B. (2006) Welfare of laying hens in furnished cages and in non-cage systems. Proc. 40th Int. Cong. ISAE, Bristol (GB): p.102. Rodenburg T.B., Tuyttens F.A.M., De Reu K., Herman L., Zoons J., Sonck B. (2006) Welfare assessment of laying hens in furnished cages and non-cage systems: assimilating expert opinion. Proc. 12th Eur. Cong. on Companion Animal Behavioural Medicine, Gent (B): 75-78. Smet K., De Block J., De Campeneere S., De Brabander D., Herman L., Raes K., Dewettinck K., Coudijzer K. (2006) Onset of oxidation in milk: the FRAP and DPPH methods. Book of abstracts 4th Euro Fed Lipid Cong., Madrid (E): p. 361. 112 Van Coillie E., Van Immerseel F., Botteldoorn N., De Buck J., Grijspeerdt K., Herman L., Ducatelle R., Heyndrickx M. (2006) Screening of in-vitro probiotic activities of lactobacilli isolated from laying hens and in-vivo evaluation of colonization and inhibition of Salmonella Enteritidis. Book of abstracts 11th Conf. on Food Microbiology, Gent (B): p.149. Van den Bulcke M., Leunda Casi A., Mbongolo Mbella G., Lievens A., Sneyers M., Taverniers I., De Loose M., Staelens A., Merckaert C., Van Bockstaele E., Debode F., Berben G. (2006) GMO reference plasmids: development, deposit and use in the detection of GM food/feed. GMO workshop, Platform for scientific concertation: Food safety. Melle (B): 1 p. Vangeyte J., Baert J., Scheers B., Maertens W., Sonck B. (2006) Analyzing particle trajectories: assessing the distribution of the grains in the circumference of the disk. The 2nd Int. Symp. on Centrifugal Fertiliser Spreading, Montoldre (F). Vlaemynck G., Heyndrickx M., Herman L. (2006) Optimised tools/detection methods and an integrated approach, essentials to reduce food-borne pathogens in animal foodstuffs. Book of abstracts CIFST/AAFC Joint Conf.: At the forefront of agri-food innovations, Montreal (CAN): p.92. Vlaemynck G. (2006) Healthy cheeses: what about fat and salt? Cheese and health Seminar, Europel Annual Meeting, Porto (P): 13 p. Werbrouck H., Botteldoorn N., Uyttendaele M., Herman L., Van Coillie E. (2006) Real time reverse transcription PCR for quantification of virulence gene expression of Listeria monocytogenes strains. Convention book of the Annual Flemish Biotech Convention: Knowledge for growth, Gent (B): p. 350. Werbrouck H., Grijspeerdt K., Botteldoorn N., Van Pamel E., Van Damme J., Uyttendaele M., Herman L., Van Coillie E. (2006) Differential inlA and inlB expression and interaction with human intestinal and liver cells by Listeria monocytogenes strains of different origins. Book of abstracts 11th Conf. on Food Microbiology, Gent (B): p.156. 5.5 Vulgarised articles Animal Sciences Cooreman K. (2006) Oostendse tongkwekerij in zicht. Het Visserijblad, 73 (6): 3-6. De Boever J., Vanacker J., De Brabander D.L. (2006) Voederwaarde van erwten-gerstkuil. Rapport LCV: 6 p. De Brabander D.L. (2006) Système d’évaluation de la structure physique pour l’alimentation des vaches laitières. Brochure Solvay - Carbonate - France. Decaestecker E., De Campeneere S., Van Looveren M. (2006) Boer voedt koe goed. Melkureum helpt rantsoen optimaliseren. Brochure van het ALT-demo project “Boer voedt koe goed”: 31 p. Delbare D. (2006) Hangmosselen voor de Belgische kust. De Grote Rede, 16: p. 29. Demaré W., Vanhee W., Redant F. (2006) Vangstvooruitzichten 2007. Rederscentrale, November 2006. Depestele J., Polet H., Van Craeynest N. (2006) Commerciële kieuw- en warrelnetvisserij. De Grote Rede, 17: 2-6. Fiems L.O. (2006) Dikbilkalveren zogen of opfokken? Landbouw & Techniek, 13 jan.: 4-6. Lippens M., Huyghebaert G. (2006) Verbetering van de n-efficiëntie bij vleeskippen: zijn er nog mogelijkheden? In: Infonamiddag. Eenheid DIER, Melle, 15 juni: 13-22. Maertens L. (2006) A házinyúl tejtermelése. Nyúltenyésztés, 2: 68-72. Maertens L. (2006) Nuove conoscenze riguardanti l’alimentazione del coniglio. Revista di Coniglicoltura, 5: 12-15. Maertens L., Aerts J., De Brabander D.L. (2006) Invloed van een voeder rijk aan n-3 vetzuren op prestaties en inbouw in melk en vlees bij het konijn als modeldier. In: Infonamiddag. Eenheid DIER, Melle, 15 juni: 5-12. Nijs G. (2006) Speuren naar varkenswereld zonder berengeur. Varkensbedrijf, 17 (9): 18-19. Tuyttens F., Van Nuffel A., Heyndrickx M., de Boeck M., Moreels A., Van Poucke E., Van Coillie E., Van Dongen S., Lens L. (2006) Biokuiken voelt zich beter - Vergelijkend onderzoek op 14 Belgische bedrijven. Pluimveehouderij, 1 juli: 18-19. Tuyttens F., Van Poucke E., Van Nuffel A., Heyndrickx M., Van Coillie E., de Boeck M., Moreels A., Lens L., Van Dongen S. (2006) Vergelijking van de gezondheid en het welzijn van vleeskippen in biologische versus conventionele productiesystemen. PCBT - Interprovinciaal Proefcentrum voor de Biologische Teelt - Overzicht van het onderzoek 2005 - Provincie West-Vlaanderen: 128130. Van Oeckel M.J., Warnants N., De Paepe M., Vanacker J., De Boever J., De Brabander D.L. (2006) Ad libitum voederopname bij drachtige zeugen: hoe voorspellend zijn de chemische en fysische eigenschappen van de rantsoenen? In: Infonamiddag. Eenheid DIER, Melle, 15 juni: 23-33. Willekens K., Van Waes J., Carlier L. (2006) Resultaten van proeven in 2005 met kuilmaïsrassen op 3 biologische bedrijven. Brochure Landbouwcentrum Voedergewassen, 2006: 51-54. Warnants N., Van Oeckel M.J., De Paepe M., Millet S., De Brabander D.L. (2006) Evolutie van de lichaamssamenstelling van big tot slachtvarken. In: Infonamiddag. Eenheid DIER, Melle, 15 juni: 35-50. Bette L., Lauwers N., Lindebrings A., Maeghe L. (2006) Focus op veiligheid en gezondheid. Landbouw en Techniek, 11(17): 33-47. Social Sciences Van Huylenbroeck G., De Cock L., Krosenbrink E., Mondelaers K., Lauwers L., Kerselaers E., Govaerts W. (2006) Biologische landbouw: Mens, Markt en Mogelijkheden. In: Delanote L., Vuylsteke I., Temmerman F., Danckaert F., Demeulemeester M., Calus A. (Eds) Biologische landbouw in Vlaanderen: Overzicht van het onderzoek 2005, PCBT, Rumbeke-Beitem: 14-15. Van Meensel J., Lauwers L. (2006) Blijven winnen: de uitdaging voor de varkenshouder vandaag. VEVA krant, juni 2006: 8-9. Van Meensel J., Lauwers L. (2006) Verbeteren van economische en milieukundige bedrijfsprestaties. VEVA krant, november 2006. Technology & Food Bette L. & Maeghe L. (2006) In vuur en vlam: liever niet in de land- en tuinbouw. Agriconstruct, 9(3): 16-22. Boussery K. (2006) Mestopslag volgens Vlarem II. Agriconstruct, 9(1): 7-11. Boussery K. (2006) Hoe een mestopslag bouwen voor vaste mest. Agriconstruct, 9(1): 12-16. Boussery K. (2006) Mengmestkelders en mestsilo’s. Agriconstruct, 9(1): 17-19. Boussery K. (2006) De grote evaluatie-enquête in cijfers en figuren. Agriconstruct, 9(1): 26-27. Plant Sciences Boussery K. (2006) Hoe ruw is uw opgeruwde roostervloer? Melkveebedrijf, 6(2): p.33. Coomans D., Rombouts G., De Vliegher A., Martens D. (2006) Een succesvolle weidevernieuwing begint met ene bewuste rassenkeuze. Brochure Landbouwcentrum Voedergewassen, 2006: 78-90. Boussery K. (2006) Comfortabel op stal. Landbouw & Techniek, 11(06): 1114. De Keyser E. (2006) Innovatief (Nieuwsbrief van het Azalea – Innovatiefonds), jg.2 nr. 2. De Keyser E. (2006) Innovatief (Nieuwsbrief van het Azalea – Innovatiefonds), jg. 3 nr. 1. De Keyser E. , De Riek J. (2006) Oosterse schoonheid aan de oevers van het Lago Maggiore, Verbondsnieuws nr.11: 39-40. De Rocker E., Goen K., Van Poucke K., Ingels M. (2006) Ontsmetten van drainwater bij tomaat. Proeftuinnieuws 16 (10): 17-19. De Vliegher A.(2006) Rassenproeven voederbieten. Brochure Landbouwcentrum Voedergewassen, 2006: 98-102. De Vliegher A. (2006) Voederbieten in de rundveevoeding. Landbouwleven, 2584: p.12. (in 3 delen) Huvenne H., Van Vaerenbergh J., Baeyen S., Maes M. (2006) Bloedingsziekten door aantastingen van bast en hout. Vakblad voor Tuinaannemers en Groenverzorgers, april: 11-13. Lamote V. (2006) Nieuwsbrief Sietinet, 2 nr. 1. Boussery K. (2006) Aandachtspunten bij de constructie van een mestopslag. Landbouw & Techniek, 11(8): 24-27. Boussery K. (2006) Hoe het koecomfort in de ligboxstal verbeteren? Drietandmagazine, 18: 1-18. Boussery K. (2006) Dierenwelzijn en ammoniakemissie, twee struikelblokken bij nieuwbouw in de varkenshouderij. Drietandmagazine, 18: 22-24. Boussery K. (2006) Methoden voor opruwen roostervloer. Agrabeton, 16(3): 17-18. Boussery K. & Ployaert C. (2006) Kiezen voor een mestkelder of een externe mestsilo? Landbouw & Techniek, 11(12): 21-22. Boussery K. (2006) Ruimte voor creatieve ideeën. Landbouw & Techniek, 11(12): 44-45. Boussery K. (2006) Is de investering in een robotstal groter dan een visgraatstal? Drietandmagazine, 34: 12-13. De Boosere I., Coudijzer K., De Ville W., De Vreese L. (2006) Sectorgids Hoevezuivel. Drietandmagazine, 34: 8-9. Lamote V. (2006) Nieuwsbrief Sietinet, 2 nr. 2. De Boosere I., Coudijzer K., Vlaemynck G. (2006) Zuursels in zuivelbereiding: Wat zijn ze? Wat doen ze? Landbouw & Techniek, 11: 31-34. Lamote V. (2006) Nieuwsbrief Sietinet, 2 nr. 3. De Boosere I., Coudijzer K., Vlaemynck G. (2006) Pro- en prebiotica in zuivelproducten. Landbouw & Techniek, 18: 12-15. Lamote V. (2006) Sierteelt Technologie en Innovatie Netwerk / Sietinet evalueert 2 jaar werking, Verbondsnieuws 9. Leus L. (2006) Nieuwsbrief Sietinet, 2 nr. 4. Mertens J., Bries J., De Vliegher A. (2006) Zwavel- en seleniumbemesting bij grasland. Brochure Landbouwcentrum Voedergewassen, 2006: 72-77. Recirculatie van water in de glastuinbouw. (2006) Ministerie van de Vlaamse Gemeenschap. Beleidsdomein Landbouw en Visserij. Mede-redacteur Kris Van Poucke. Viaene N., Cornelissen K. (2006) Biofumigatie ter beheersing van nematoden in de biologische teelt van komkommer. In: Biologische landbouw in Vlaanderen. Overzicht van het onderzoek 2005 – PCBT: 102-107. De Boosere I., Wullepit J., Herman L., Coudijzer K., Demeulemeester L. (2006) Bedrijfsmanagement speelt een grote rol in de strijd tegen paratuberculose. Landbouw & Techniek, 13: 32-34. De Rooster L., Spiessens K., Dierickx W, Van Den Berghe (2006) Wildschade bij vollegrondsgroenten: afdekken biedt mogelijkheden. Proeftuinnieuws, 16(8): 31-34. Dierickx W. (2006) Drainage van landbouwgronden. Agromech. Nieuwsbrief 3. Lindebrings A., Bette L., Lauwers N., Maeghe L. (2006) Veilig omgaan met paarden: hoe ervaren zij hun omgeving? Haflinger Magazine, 4: 11-16. Willekens K. (2006) Bodembiologie is hoofdfactor voor land- en tuinbouw. Landbouw&Techniek 4, 21-22. Nuyttens D. (2006) Drift en driftreducerende technieken – Stand van zaken onderzoek. 30ste fytodag ‘Driftreductie en actualiteiten bij gewasbeschermingsmiddelen’, VOLSOG, Gent: 14-17. Willekens K. (2006) Voorstelling van de TAD FarmCOMPOST, Landbouw & Techniek 12: p 46. Nuyttens D. (2006) Driftreductie mogelijk mits goede keuze van doppen en spuitdruk. Landbouw & Techniek, 7: 7-9. Willekens K. (2006) Boerderijcompost - dierlijke mest: 1-0, Boer&Tuinder, 26: p 10. Nuyttens D. (2006) Welke invloed hebben weersomstandigheden op drift? Landbouw & Techniek, 6: 7-9. Willekens K. (2006) Compost en compostthee in de boomkwekerij, Verbondsnieuws, 2: 28-29. Nuyttens D., Windey S., Sonck B. (2006) Report: Comparison of operator contamination between two application methods in greenhouses in Greece. ECPA Safe Use Initiative: 41 p. Willekens, K. (2006) De invloed van de bemesting op de knolkwaliteit, % rot tijdens de bewaring en % niet uitlopers bij de gezonde knollen voor Begonia x tuberhybrida, Sierteeltonderzoek in Vlaanderen 2005, 2005/49. Willekens K., Van Waes J., Carlier L. (2006) Resultaten van proeven in 2005 met kuilmaïsrassen op 3 biologische bedrijven. Biologische landbouw in Vlaanderen: 47-51. Reybroeck W., Ooghe S., Van Hoorde A., Jacobs F.J. (2006) Honinganalyses 2005. Maandblad van de Vlaamse Imkersbond, 92(7): 29-32. Reybroeck W., Ooghe S., Van Hoorde A., Jacobs F.J. (2006) Honinganalyses in Vlaanderen anno 2005. Vlaams Imkersblad, 36(9): 305-312. Rodenburg T.B., Tuyttens F.A.M., De Reu K., Herman L., Zoons J., Sonck B. (2006) Welzijn, gezondheid en sanitaire status van leghennen in verrijkte kooien versus niet-kooisystemen. Agriconstruct, 9(3): 23-27. 113 Rodenburg T.B., Tuyttens F.A.M., De Reu K., Grijspeerdt K., Herman L., Zoons J., Sonck B. (2006) Welzijn, gezondheid en sanitaire status van leghennen in verrijkte kooien versus niet-kooisystemen: resultaten bedrijfbezoeken. Agriconstruct, 9(4): 17-22. Rodenburg T.B., Tuyttens F.A.M., De Reu K., Herman L., Zoons J., Sonck B. (2006) Onderzoek naar welzijn en gezondheid legkippen: Verrijkte kooi versus niet-kooi. Pluimveehouderij, 36(42): 28-29. Rodenburg T.B., Tuyttens F.A.M., De Reu K., Herman L., Zoons J., Sonck B. (2006) Rubriek: Dierenwelzijn. Welzijn, gezondheid en sanitaire status van leghennen in verrijkte kooien versus niet-kooisystemen. Available at: http://www.pluimvee.be/nieuws/pluimvee%2010%20welzijn%20kippen.htm. Rodenburg T.B., Tuyttens F.A.M., De Reu K., Herman L., Sonck B. , Zoons J. (2006) Legkippen in verrijkte kooien versus niet-kooisystemen. Landbouw & Techniek, 11(18): 20-23. Tuyttens F.A.M., Van Nuffel A., Heyndrickx M., De Boeck M., Moreels A., Van Poucke E., Van Coillie E., Van Dongen S., Lens L. (2006) Vergelijking van de gezondheid en het welzijn van vleeskippen in biologische versus conventionele productiesystemen. Agriconstruct, 9(2): 29-31. Vangeyte J., Hubrechts W., Hendrickx K., Aerts R. (2006) Beschadiging van witloofwortels en het optreden van Phoma (zwartrot). Proeftuinnieuws, 23: p. 29. Van Immerseel F., Timbermont L., Van Coillie E., Botteldoorn N., Messens W., De Buck J., Herman L., Haesebrouck F., Heyndrickx M., Ducatelle R. (2006) Pathogenese en bestrijding van Salmonella bij leghennen en vleeskippen. Publicatie van de FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu, Contractueel Onderzoek: 147 p. 5.7 ILVO communications Animal Sciences Moulaert I., Hostens K., Demaerel B., Wittoeck J., Hillewaert H. (2006) Macrobenthos as an indicator of the impact of sand extraction. Mededeling ILVO nr. 6. Moulaert I., Hostens K., Wittoeck J., Hillewaert H., Demaerel B. (2006) Langetermijn variabiliteit van het macro-endo- en epibenthos van het Belgisch Continentaal Plat. Mededeling ILVO nr. 7. Social Sciences Lenders S., Lauwers L., Vervloet D., Kerselaers E. (2006) Afbakening van het Vlaamse platteland –een statistische analyse. Mededeling ILVO nr. 2: 62 p. Plant Sciences Baert J., Van Eekeren N., Ghesquiere A. (2006) Breeding fodder grass and clover for low input/organic conditions in N.W. Europe. Mededeling ILVO nr. 8. Baert J., Ghesquiere A. (2006) Comparison between two breeding methods in perennial ryegrass: polycross versus F2. Mededeling ILVO nr. 9. Carlier L., De Roo I., Vlahova M. (2006) Manuals for the organic production system (Bulgaria). Mededeling ILVO nr. 10: 112 p. Windey S. (2006) Agromech nieuwsbrief 3. Carlier L., De Roo I., Rotar I. (2006) Manuals for the organic production system (Romania). Mededeling ILVO nr. 11: 112 p. Windey S. (2006) Eerste hulp voor machines. Landbouw & Techniek 12: 4243. De Vliegher A., Van Waes J., Carlier L. (2006) Cultivation of red clover and lucerne I. Mededeling ILVO nr. 14: 66 p. Windey S. (2006) Een blik op het voorbije aardappel rooiseizoen: de rooiertesten van 2005. FIWAP jaarverslag 2005. De Vliegher A., Van Waes C., Carlier L. (2006) Metodiskais materiāls precīzai lopbarības analīžu noteikšanai. Mededeling ILVO nr. 16: 46p. Windey S., Goossens E., Nuyttens D. (2006) Spuittechniek: indringing in dichte gewassen. Jaarverslag sierteelt ABKL 2005. Duquenne B., Eeckhaut T., Werbrouck S., Van Huylenbroeck J. (2006) In-vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids. Mededeling ILVO nr. 15. Windey S., Maeghe L. (2006) Van frigo tot oude kast: opslag van gewasbeschermingsmiddelen. Agriconstruct 9(2): 24-28. 5.6 Ph. D. theses Animal Sciences Delezie E. (2006) Manual and mechanical catching and transport of broilers: implications for welfare, physiology and product quality and ethical considerations. Doctoraatsproefschrift nr. 721 aan de faculteit Bio-Ingenieurswetenschappen van de K.U.Leuven: 193 p. Promotoren Prof. E. Decuypere en Prof. J. De Tavernier. Plant Sciences de la Peña E. (2006) Interactions between marram grass (Ammophila arenaria), root-lesion nematodes (Pratylenchus spp.) and plant mutualists in coastal dunes. PhD Thesis Ghent University, ISBN 90-5989-119-8, 148p. Promotor Prof. M. Moens. Rijckaert G. (2006) Selectivity of fluroxypyr + clopyralid + florasulam and reference products to a seed crop of perennial ryegrass (harvest 2005), Confidential report. Mededeling ILVO nr.12. Rijckaert G. (2006) Crop safety in a seed crop of perennial ryegrass (Lolium perenne L.) after application of aminopyralid, Confidential report. Mededeling ILVO nr. 13. Van Bockstaele E. (2006) Moderne plantenveredeling – Relatie kruisingsveredeling en transgenen. Mededeling ILVO nr. 1. Van Waes J., Chaves B., Marynissen B., De Vliegher A., Carlier L. (2006) Belgische beschrijvende en aanbevelende rassenlijst voor voedergewassen en groenbedekkers-2007. Mededeling ILVO nr. 3: 113 p. Van Waes J., Chaves B., Marynissen B., De Vliegher A., Carlier L., Herman J-L. (2006) Catalogue Belge-Description et recommandation-Plantes fourragères et engrais verts-2007. Mededeling ILVO nr. 4: 113 p. Van Waes J., De Bel N., Carlier L., Van Waes C. (2006) Belgische beschrijvende en aanbevelende rassenlijst voor industriële cichorei. Mededeling ILVO nr. 5: 7p. Madani M. (2006) Molecular characterisation, phylogeny and quantification of Heterodera and Globodera species and the Ditylenchus species complex. PhD Thesis Ghent University, ISBN 90-5989-123-6, 156 p. Promotor Prof. M. Moens. Pudasaini M.P. (2006) Interactions between the root lesion nematode, Pratylenchus penetrans, and field crops. PhD Thesis Ghent University, ISBN 90-5989122-8, 185 p. Promotor Prof. M. Moens. Technology & Food Botteldoorn N. (2006) Salmonella in pigs: prevalence and contamination cycles at the slaughterhouse and the differentiation of Salmonella enterica on the basis of the mntH gene expression. Doctoraat, UGent, FDI, 173 p., ISBN 90-5864093-0. Promotoren Prof. F. Pasmans en L. Herman, co-promotor M. Heyndrickx. De Reu K. (2006) Bacteriological contamination and infection of shell eggs in the production chain. Doctoraat, UGent, FBW: 173 p. ISBN 90-5989-124-4. Promotoren Prof. M. Uyttendaele en L. Herman. 5.8 Policy-relevant notes and reports in the framework of mandated and other tasks Animal Sciences Anon. (2006) Datacollectie met betrekking tot de aanlandingen en de teruggooi van de boomkorvisserij in de Ierse Zee - 2005 - Projectverslag. Intern activiteitenverslag MVG (eindredactie Frank Redant, bijdrage Wim Demaré). Anon. (2006) National Data Gathering Programme under EC Regulation 1639/2001 amended by EC Regulation 1581/2004 - Belgium - 2005 - Technical report. Activiteitenverslag ingediend bij de Europese Commissie (eindredactie Frank Redant, bijdrage Wim Demaré en Bart Maertens). Anon. (2006) Reply to the request by the European Commission for further information on the 2005 Technical Report. Aanvulling op het activiteitenverslag ingediend bij de Europese Commissie (eindredactie Frank Redant, bijdrage Wim Demaré). 114 Anon. (2006) Report of the Steering Group on the Discard Atlas. Ispra (IT) (bijdrage Frank Redant). Anon. (2006) Regional Co-ordination Meeting (RCM) for the North Sea and East Arctic, 2006. Den Haag (NL) (bijdrage Frank Redant, Bart Maertens). Anon. (2006) Third Regional Co-ordination Meeting for the North East Atlantic Area. Lisbon (PT) (bijdrage Frank Redant, Bart Maertens). Anon. (2006) Training Workshop on Fleet-based Approach. Nantes (FR), Commission Staff Working Paper (bijdrage Bart Maertens). SEC 2006 XX. Anon. (2006) Report of the Ad Hoc Meeting of Independent Experts on FleetFishery based sampling. Nantes (FR), Commission Staff Working Paper (bijdrage Bart Maertens). (SEC 2006 XX). Anon. (2006) Report of the 2nd Liaison Meeting between the Chairs of the RCMs, the Chair of SGRN and the European Commission. Brussel (B) (eindredactie Frank Redant). De Maersschalck V., Hostens K., Wittoeck J., Cooreman K., Vincx M., Degraer S. (2006) Monitoring van de effecten van het Thornton windmolenpark op de benthische macro-invertebraten en de visfauna van zachte substraten – referentietoestand: Eindrapport september 2006. Rapport Dier-Visserij/Monitoring/2006-02, 136 p. Depestele J., Delanghe F., Vanhee J., Van Craeynest N., Vanden Berghe C. (2006) Tussentijds rapport Project Alternatieve Visserij (PAV). Project nr. VIS/02/ B/07/DIVb,. studie uitgevoerd met financiële steun van de Vlaamse Gemeenschap en de Europese Commissie (FIOV). Promotor: Rederscentrale N.V. 28 p. Depestele J., Stouten H., Van Craeynest K., Van Craeynest N., Vanderperren E., Polet H. (2006) Tussentijds rapport Project Alternatieve Visserij (PAV). Project nr. VIS/02/B/07/DIVb, studie uitgevoerd met financiële steun van de Vlaamse Gemeenschap en de Europese Commissie (FIOV). Promotor: Rederscentrale N.V. 47 p. Derous S., Degraer S., Verfaillie E., Van Lancker V., Courtens W., Stienen E., Hostens K., Moulaert I., Hillewaert H., Mees J., Deneudt K., Deckers P., Cuvelier D., Vincx M. (2006) BWZee: A biological valuation map for the Belgian part of the North Sea. Final report. PODO-II, Belspo-project, 70 p. ICES (2006) Report of the Annual Meeting of Assessment Working Group Chairs (AMAWGC). International Council for the Exploration of the Sea, Advisory Committee on Fishery Management (bijdrage Wim Demaré, D-VI). ICES CM 2006/ACFM:17. ICES (2006) Report of the Benthos Ecology Working Group (BEWG). International Council for the Exploration of the Sea, Advisory Committee on Ecosystems (bijdrage Hans Hillewaert, Ine Moulaert, D-VI). ICES CM 2006/MHC:09. ICES (2006) Report of the ICES Advisory Committee on Fishery Management, the Advisory Committee on the Marine Environment and the Advisory Committee on Ecosystems, 2006 (ACFM, ACME and ACE) (bijdrage Wim Demaré, Willy Vanhee). ICES Advice, Books 1-10. ICES (2006) Report of the ICES-FAO Working Group on Fishing Technology and Fish Behaviour (WGFTFB). International Council for the Exploration of the Sea, Advisory Committee on Fisheries Management (bijdrage Hans Polet, Els Van der Perren, Jochen Depestele, Hendrik Stouten). ICES CM 2006/FTC:06. ICES (2006) Report of the Planning Group on Commercial Catch, Discards and Biological Sampling (PGCCDBS). International Council for the Exploration of the Sea, Advisory Committee on Fishery Management (bijdrage Frank Redant). ICES CM 2006/ACFM:18. ICES (2006) Report of the Study Group on Management Strategies (SGMAS). International Council for the Exploration of the Sea, Advisory Committee on Fishery Management (bijdrage Wim Demaré). ICES CM 2006/ACFM:15. ICES (2006) Report of the Study Group on the North Sea Benthos Project 2000 (SGNSBP). International Council for the Exploration of the Sea, Advisory Committee on the Marine Environment (bijdrage Hans Hillewaert) ICES CM 2006/ MHC:06. ICES (2006) Report of the Working Group on Beam Trawl Surveys (WGBEAM). International Council for the Exploration of the Sea, Living Resources Committee (bijdrage Bart Maertens). ICES CM 2006/LRC:11. ICES (2006) Report of the Working Group on Environmental Interactions of Mariculture (WGEIM). International Council for the Exploration of the Sea, Mariculture Committee (bijdrage Daan Delbare). ICES CM 2006/MCC:03. ICES (2006) Report of the Working Group on Marine Shellfish Culture (WGMASC). International Council for the Exploration of the Sea, Mariculture Committee (bijdrage Daan Delbare). ICES CM 2006/MCC:02. ICES (2006) Report of the Working Group on the Assessment of Northern Shelf Demersal Stocks (WGNSDS). International Council for the Exploration of the Sea, Advisory Committee on Fishery Management (bijdrage Willy Vanhee). ICES CM 2006/ACFM:30. ICES (2006) Report of the Working Group on the Assessment of Demersal Stocks in the North Sea and Skagerrak (WGNSSK). International Council for the Exploration of the Sea, Advisory Committee on Fishery Management (bijdrage Willy Vanhee, Frank Redant). ICES CM 2006/ACFM:35. ICES (2006) Report of the Working Group on the Assessment of Southern Shelf Demersal Stocks (WGSSDS). International Council for the Exploration of the Sea, Advisory Committee on Fishery Management (eindredactie Wim Demaré, bijdrage Willy Vanhee). ICES CM 2006/ACFM:33. ICES (2006) Report of the Working Group on the Effects of Extraction of Marine Sediments on the Marine Ecosystem (WGEXT). International Council for the Exploration of the Sea, Advisory Committee on the Marine Environment (bijdrage Kris Hostens, Hans Hillewaert, Ine Moulaert). ICES CM 2006/MHC:07. ICES (2006) Report of the Workshop on Simple Mixed Fisheries Management Models (WKMIXMAN). International Council for the Exploration of the Sea, Advisory Committee on Fishery Management (bijdrage Willy Vanhee). ICES CM 2006/ACFM:14. ICES (2006) Report of the Workshop on Nephrops Stocks (WKNEPH). International Council for the Exploration of the Sea, Advisory Committee on Fishery Management (bijdrage Frank Redant). ICES CM 2006/ACFM:12. Lauwaert B., De Brauwer D., Fettweis M., Hillewaert H., Hostens K., Mergaert K., Moulaert I., Parmentier K., Verstraeten J. (2006) Syntheserapport over de effecten op het mariene milieu van baggerstortingen (vergunningsperiode 2004’06). Rapport BMM-ILVO-aMT, BL/2006/01, 87 p. Moulaert I., Hostens K., Parmentier K., Bekaert K., Hoffman S., Hillewaert H. (2006) Voortgangsrapportage baggeren Periode 1 Juli 2005 – 31 December 2005. Uitvoering van de werkzaamheden opgenomen in het protocol tussen het Centrum voor Landbouwkundig Onderzoek – Departement Zeevisserij (CLO/ DvZ) en de Administratie Waterwegen en Zeewezen – Afdeling Maritieme Toegang, ondertekend op datum van 5 september 2003. Rapport DVZ-Bagger 2005/2, 7 p. Polet H., Depestele J., Vanderperren E., Buyvoets E., Vanden Berghe C., Vancraeynest N. (2006) Projectverslag SDVO Project “Tweelingboomkor” en SDVO Project “Alternatieve boomkor”. Tussentijds rapport. Studie uitgevoerd met financiële steun van de Stichting Duurzame Visserijontwikkeling vzw. 32 p. STECF (2006) Report of the Scientific, Technical and Economic Committee for Fisheries (spring meeting). Brussel (B), Commission Staff Working Paper (bijdrage Willy Vanhee). SEC 2006 XX. STECF (2006) Report of the Scientific, Technical and Economic Committee for Fisheries (autumn meeting). Ispra (IT), Commission Staff Working Paper (bijdrage Willy Vanhee). SEC 2006 XX. STECF/SGEC-SGRST (2006) Report of Review of Scientific Advice for 2007. Brussel (B), Commission Staff Working Paper (bijdrage Willy Vanhee). SEC 2006 XX. STECF/SGRN (2006) Report of the Sub-Group on Research Needs (summer meeting). Ispra (IT), Commission Staff Working Paper (bijdrage Frank Redant). SEC 2006 XX. STECF/SGRN (2006) Report of the Sub-Group on Research Needs (winter meeting). Brussel (B), Commission Staff Working Paper (bijdrage Frank Redant). SEC 2006 XX. STECF/SGRN (2006) Revision of the Biological Data Requirements under the EU Data Collection Regulation. Brussel (B), Commission Staff Working Paper (eindredactie Frank Redant). (SEC 2006 XX). STECF/SGRST (2006) Report on Fishing Effort Management. Ispra (IT), Commission Staff Working Paper (bijdrage Willy Vanhee). SEC 2006 XX. STECF/SGRST (2006) Report on Mixed Fisheries and Fishing Effort Regimes in Recovery and Management Plans. Ispra (IT), Commission Staff Working Paper (bijdrage Willy Vanhee). SEC 2006 XX. STECF/SGRST (2006) Report on the Impact Assessment of Plaice and Sole Long�term Management. Brussel (B), Commission Staff Working Paper (bijdrage Willy Vanhee). SEC 2006 XX. STECF/SGRST (2006) Report on Mixed Fisheries. Lisbon (PT), Commission Staff Working Paper (bijdrage Willy Vanhee). SEC 2006 XX. Vanaverbeke J., Béllec V., Bonne W., Deprez T., Hostens K., Moulaert I., Van Lancker V., Vincx M. (2006) SPEEK: study of the post-extraction ecologial effects in the Kwintebank sand dredging area. Final Report. PODO-II Belspo project, 80 p. Vanderperren E. (2006) Tussentijds rapport: introductie van bordenvisserij in de boomkorvloot met het oog op brandstofbesparing. Project nr. VIS/06/C/02/ DIV, studie uitgevoerd met financiële steun van de Vlaamse Gemeenschap, Europese Commissie (FIOV) en Stichting Duurzame Visserijontwikkeling vzw. Promotor: Stichting Duurzame Visserijontwikkeling vzw. 38 p. N and P excretion of dairy cows (De Brabander, 4 p.), of suckling cows and growing and finishing cattle (Fiems, 3 p.), of piglets, meat pigs and sows (Van Oeckel, 5 p.), of poultry (Huyghebaert, 3 p.), of rabbits (Maertens, 1 p.). Nota opgemaakt voor het Kabinet van de Vlaams Minister van Landbouw, Zeevisserij en Plattelandsbeleid, maart 2006. 115 Aanpak van de N- en P-excretie via de voeding. Presentatie door D. De Brabander voor de Commissie Leefmilieu van het Vlaams Parlement, 22 juni 2006. N-excretion by dairy cattle. Bemerkingen op het standpunt van de Europese Commissie over de uitscheidingsnormen bij melkvee, opgemaakt door D. De Brabander voor het Kabinet van de Vlaams Minister van Landbouw, Zeevisserij en Plattelandsbeleid, oktober 2006. Justification of the excretion figures of dairy cows. Nota opgemaakt door D. De Brabander voor het Kabinet van de Vlaams Minister voor Leefmilieu en Natuur, 1 december 2006. Bemerkingen en suggesties bij het ontwerp-besluit van Mestdecreet op basis van de opmerkingen van de Europese Commissie. Nota opgemaakt door D. De Brabander voor het Kabinet van de Vlaams Minister voor Leefmilieu en Natuur, 22 december 2006. Chirurgische castratie en bijbehorende problematiek. Nota opgemaakt door Eenheid Dier, Melle voor het Kabinet van de Vlaams Minister van Landbouw, Zeevisserij en Plattelandsbeleid, 28 november 2006. Social Sciences Buysse J., Fernagut B., Van Meensel J., Vanhuylenbroeck G., Lauwers L. Geïntegreerd systeem voor evaluatie van landbouw- en agromilieupolitiek - SEPALE. Activiteitenverslag 2004-2006, IWT-project 020677, Instituut voor Landbouwen Visserijonderzoek, Merelbeke: 74 p. Claeys D., Van Lierde D., Zoons J., Rodenburg B., Tuyttens F. (2006) Studie naar de socio-economische gevolgen van verschillende huisvestingssystemen in de leghennenhouderij. Eindrapport i.o.v. ADLO uitgevoerd door ILVO, Eenheid L&M, Merelbeke in samenwerking met ILVO, Eenheid Dier, Merelbeke en het Proefbedrijf voor de Veehouderij, Geel: 226 p. De Mey K., De Cock L., Van Lierde D. (2006) Studie naar de mogelijkheden tot optimalisatie en structurering van het Vlaamse landbouwonderzoek in de biologische sector en tot het afstemmen van het onderzoek en ontwikkeling in de biologische landbouw op de noden van de Vlaamse biologische landbouw. Eindrapport i.o.v. ADLO uitgevoerd door het ILVO, Eenheid Landbouw en Maatschappij in samenwerking met ILVO, Eenheid Dier, Eenheid Plant en Eenheid Technologie en Voeding, Merelbeke: 119 p. Fernagut B., Wustenberghs H., Lauwers L. (2006) Nutriëntenexcretie door melkvee. Geactualiseerde coëfficiënten in dynamisch perspectief. Studie uitgevoerd in opdracht van de Vlaamse Milieumaatschappij, Instituut voor Landbouw en Visserijonderzoek, Eenheid Landbouw en Maatschappij, Merelbeke: 62 p. Kerselaers E., Lauwers L. (2006) Certificering van het Vlaamse PDPO 20072013. Nota voor Departement Landbouw&Visserij. Instituut voor Landbouw en Visserijonderzoek, Eenheid Landbouw en Maatschappij, Merelbeke: 60 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Inventarisatie van reductiemogelijkheden voor het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten – subsector sierteelt. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 58 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Analyse en evaluatie van reductiemogelijkheden voor het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten – subsector sierteelt. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 218 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Analyse en evaluatie van reductiemogelijkheden voor het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten – subsector fruitteelt. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 153 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Evaluatie van gegevens over het gebruik van gewasbeschermingsmiddelen en nutriënten in de Vlaamse tuinbouw door vergelijking met gegevens uit andere landen. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 40 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Nutriënten en gewasbeschermingsmiddelengebruik in de Vlaamse tuinbouw: stand van zaken en reductiemogelijkheden. Instituut voor Landbouw- en Visserijonderzoek, eindrapport ALT-project 2003/07: 130 p. Plant Sciences Carlier L. & Van Waes J. (2006) Cooperation Flanders C&E Europe (project “Slovarseed” SLO/001/02). Carlier L. et al. (2006) Yearly newsletter 2: “Reduced tillage” project BUL/001/03. Carlier L. et al. (2006) Yearly newsletter 3: “Reduced tillage” project BUL/001/03. Chaves B., Marynissen B. & Carlier L. (2006) Korrelmais CGW proeven 2006. 36 p. Chaves B., Marynissen B. & Carlier L. (2006) Silomais CGW proeven 2006. 54 p. Chaves B., Marynissen B. & Carlier L. (2006) Vezelvlas CGW proeven 2006. 17 p. Kerselaers E., Lauwers L. (2006) Relevance, utility and data availability of agricultural (and forestry) competitiveness indicators and agri-environmental indicators for rural development and policy analysis. TAPAS 2006 Rural Development. Intermediate Report v 0.1. December 2006. Instituut voor Landbouw- en Visserijonderzoek, Eenheid Landbouw en Maatschappij, Merelbeke: 48 p. De Bel N. & Van Waes J. (2006) Voederbieten CGW proeven en OHB-proef 2005. 19 p. Lauwers L., Kerselaers E., Kruseman G., Terluin I. (2006) Contribution for Belgium and Luxembourg to ‘An evaluation of the Less Favoured Area Measure in the 25 member states of the European Union’. Report prepared by the Institute for European Environmental Policy for DG Agriculture. London, UK: 249 p. De Bel N., Van Waes J. & De Vliegher A. (2006) Rassenproeven met witte klaver CGW proeven 2005. 8 p. Vervaet M., Kerselaers E., Claeys D., Vandermersch M., Lenders S., Lauwers L., Wustenberghs H., Fernagut B. (2006) Operationalisation of AEI-calculation models. Execution report, TAPAS 2004 Agri-environmental indicators. Merelbeke, Instituut voor Landbouw- en Visserijonderzoek: 197 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Inventarisatie van gegevens over het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten in de Vlaamse tuinbouw. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 256 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Inventarisatie van reductiemogelijkheden voor het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten – subsector glasgroenten. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 63 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Analyse en evaluatie van reductiemogelijkheden voor het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten – subsector grondloze glasgroenteteelt. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 161 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Analyse en evaluatie van reductiemogelijkheden voor het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten – subsector grondgebonden glasgroenteteelt. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALTproject 2003/07: 149 p. Vandenberghe A., Cools A., Van Lierde D. (2006) Inventarisatie van reductiemogelijkheden voor het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten – subsector vollegrondsgroenteteelt. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 51 p. 116 Vandenberghe A., Cools A., Van Lierde D. (2006) Analyse en evaluatie van reductiemogelijkheden voor het gebruik en/of de milieurisico’s van gewasbeschermingsmiddelen en nutriënten – subsector vollegrondsgroenteteelt. Instituut voor Landbouw- en Visserijonderzoek, tussentijds rapport ALT-project 2003/07: 159 p. De Bel N., Van Waes J. & De Vliegher A. (2006) Rassenproeven met raaigrassen CGW proeven 2005. 64 p. De Vliegher A. (2006) De mogelijkheden van klaver en luzerne op het moderne melkveebedrijf. 82 p. De Vliegher A. (2006) Programma voor reductie van pesticiden en biociden van de werkgroep 10: grasland, braak en diverse teelten. 38 p. De Vliegher A., Danckaert F., Delanote L. & Carlier L. (2006) De teelt van voederbieten op het biologisch bedrijf. 46 p. Heungens K., Viaene N., Eloot B., Peeters L., Trybou M . Critical use of methyl bromide for soil disinfestation during 2005 in Belgium: Final report. 16/1/2006. Opdrachtgever/contactpersoon beleid: FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu / Maarten Trybou. Heungens K. Bespreking van het voorstel tot aanpassing van de EU beschikking rond Phytophthora ramorum. 14/12/06. Opdrachtgever/contactpersoon beleid: FAVV / Nathalie Rombaut, Vera Huyshauwer & Walter Van Ormelingen. Heungens K. Advies rond potentiële herziening van de bijlagen van EU Richtlijn 2000/29/EG m.b.t. Sirococcus clavigignenti-juglandacearum en Cylindrocladium buxicola. 13/10/06. Opdrachtgever/contactpersoon beleid: FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu / Anneleen Sarens. Lootens P., Van Waes J. & Carlier L. (2006) Het uittesten van vormbeschrijvende algoritmen en invloed van het jaareffect op de morfologische kenmerken bij industriële cichorei en knolbegonia. Rapport voor Federale overheidsdienst, Economie, KMO, Middenstand Energie, Dienst Intellectuele Eigendom 48 p. Lootens P., Van Waes J. & Carlier L. (2006) Onderzoek bij vezelvlas naar het onderscheiden van rasechte zaadpartijen en mengsels via beeldanalyse. Rapport voor L&V - ABKL 22 p. Maes M. Gevoeligheid van Rhododendron simsii voor Phytophthora ramorum. 30/05/06. Opdrachtgever/contactpersoon beleid: FAVV / Léonard Bosschaert. Mestdagh I., Lootens P., Carlier L. (2006) Carbon sequestration in grassland soils. In: Van Cleemput O., Hofman G., et al. (2006) Final report: Carbon sequestration Potential in different Belgian terrestrial ecosystems: quantification and strategic exploration (CASTEC), 99 p. 6. Theses and training reports Van Vaerenbergh J. Stengelnatrot in aardappelpootgoed. 30/11/2006. Opdrachtgever/ contactpersoon beleid: Vlaams Verbond van Pootgoedtelers. Bachelor Marynissen B. & Chaves B. (2006) EC Comparative trial for grasses. Interim Report. 54 p. Marynissen B. & Chaves B. (2006) EC Comparative trial for grasses. Final Report. 58 p. Animal Sciences Van Waes J. & De Bel N. (2006) Rassenproeven met industriële cichorei CGW proeven 2005. 25 p. Claeys S. Stage van september 2005 tot april 2006. Graduaat Landbouw – KATHO (Campus Roeselare). Begeleider: Marc De Paepe Technology & Food De Bonte L. Stage van september tot december. Bachelor in Agro- & Biotechnologie. KaHo – Sint-Lieven (Campus Sint-Niklaas). Begeleider: Frank Tuyttens Rapporten in het kader van de wetenschappelijke begeleiding van de Interprofessionele Organismen: ·Tussentijdse rapporten: “januari tot en met april 2006” (10 mei 2006) en “januari tot en met november 2006” (7 december 2006). ·Jaarverslag 2005: Wetenschappelijke begeleiding van de Belgische Interprofessionele Organismen belast met de officiële bepaling van de kwaliteit en samenstelling van rauwe melk geleverd aan kopers (14 februari 2006). Rapporten in het kader van de wetenschappelijke begeleiding van de zuivelindustrie: ·Rapport Ringonderzoek Antibiotica: sneltesten , Delvotest SP & Copan Milk Test, 16 mei 2006. ·Rapport Ringonderzoek Antibiotica: microbiologische testen (Delvotest SP-NT & CMT Copan Milk Test) en sneltesten (ROSA, SNAP, ßeta-s.t.a.r. & Parallux, 12 oktober 2006. ·Maandelijkse rapporten ringonderzoeken “Vet/eiwit in melk” en “Kiem/coli/ vriespunt in melk”. ·Tweemaandelijkse rapporten ringonderzoeken “Vet in room”. Reybroeck W., Ooghe S. (2006) Gebruik van sneltesten als bevestigingstest bij de opsporing van bacteriegroeiremmende stoffen in melk in het kader van de officiële kwaliteitsbepaling van rauwe melk. Rapport voor het Wetenschappelijk Comité van het FAVV, 2006: 22 p. Adviezen van het wetenschappelijk comité van het Federaal Agentschap voor de Veiligheid van de Voedselketen (FAVV) http://www.favv.be/home/com-sci/ avis06_nl.asp door medewerking van L. Herman als lid van het comité en E. Daeseleire, G. Vlaemynck en W. Reybroeck als externe experten in diverse werkgroepen. Adviezen van het wetenschappelijk panel ‘Genetisch gemodificeerde organismen’ van het Europees Voedselagentschap (EFSA) http://www.efsa.europa.eu/en/science/gmo/gmo_opnions.html en http://www.efsa.europa.eu/en/science/feedap/feedap_opinions.html met medewerking van M. De Loose en L. Herman als lid van het panel. Adviezen van het door de Vlaamse Regering opgerichte expertencollege voor de erkenning van voedselkwaliteitsregelingen met medewerking van L. Herman, E. Daeseleire en I. Taverniers als leden van het college. Advies van de Hoge Gezondheidsraad (FOD Volksgezondheid, veiligheid van de voedselketen en leefmilieu) betreffende de microbiologische criteria voor sommige voedingsmiddelen (HGR 8163 revisie van HGR 8114) met medewerking van M. Heyndrickx. Advies van de Hoge Gezondheidsraad (FOD Volksgezondheid, veiligheid van de voedselketen en leefmilieu) betreffende afwijking voor het Salmonella criterium in vleesbereidingen en -producten op basis van pluimveevlees die na verhitting zullen worden verbruikt (HGR 8151) met medewerking van M. Heyndrickx. Advies aan het kabinet van de Vlaams Minister van Landbouw, Zeevisserij en Plattelandsbeleid, betreffende MRSA in de Vlaamse varkenshouderij, opgesteld door M. Heyndrickx en L. Herman. Nota betreffende “Minimum vereisten voor een spoelwatertank van spuittoestellen”, op vraag van het kabinet van de Vlaams Minister van Landbouw, Zeevisserij en Plattelandsbeleid, in het kader van een promotie-actie rond spoelwatertank die zal gelanceerd worden op Agriflanders op 12 januari 2007, opgesteld door P. Braekman en B. Sonck. Verschillende adviezen voor de administratieve en wetenschappelijke commissie voor emissie-arme stalsystemen op vraag van de Vlaamse Landmaatschappij, Departement Leefmilieu van de Vlaamse Gemeenschap, in het kader van aanvragen voor nieuwe emissie-arme stalconcepten, met medewerking van K. Boussery en H. Cnockaert. Advies en medewerking aan een brochure betreffende “Agrarische architectuur” op vraag van het kabinet van de Vlaams Minister van Landbouw, Zeevisserij en Plattelandsbeleid. Lacoere T. Eiwit (IEF) en DNA (PCR-DGGE) onderzoek op vis voor identificatie. Stage 2de jaar Graduaat Chemie, KATHO Roeselare. Begeleiders: Stefan Hoffman, Sabrine Derveaux, Daphné Deloof Vennens S. Stage van september tot december. Bachelor in Agro- & Biotechnologie. KaHo – Sint-Lieven (Campus Sint-Niklaas). Begeleider: Frank Tuyttens Verburgh J. HPLC-methode voor het analyseren van biogene amines in vis. Scholengroep Sint-Rembert, Vrij Land- en tuinbouwinstituut, Torhout. Begeleiders: Sabrine Derveaux, Karen Bekaert Master De Bock G. De invloed van natriumbicarbonaat op de melkproductie en melksamenstelling. Eindwerk Industrieel Ingenieur in Landbouw en Biotechnologie, optie Landbouw, Hogeschool Gent. Promotor: Daniël De Brabander De Marez T. Onderzoek naar het nut van een vachtborstel in een melkveestal. Eindwerk Bio-ingenieur in de Landbouwkunde, Universiteit Gent. Promotoren: Frank Tuyttens en J. Christiaens Van den Bossche F. De bruikbaarheid van melkcomponenten als indicator van slepende melkziekte. Eindwerk Industrieel Ingenieur in Landbouw en Biotechnologie, optie Landbouw, Hogeschool Gent. Promotor: Daniël De Brabander Vergult S. Authenticiteitsonderzoek van commerciële vissen met behulp van PCR en DGGE. Stage 1ste proef Bioingenieur - Cel en Gen technologie. Begeleiders: Stefan Hoffman, Sabrine Derveaux, Daphné Deloof Plant Sciences Bachelor Coghe G. Evaluatie van methoden voor DNA extractie uit sla via real-time PCR detectie van toegevoegde merkerbacteriën. Graduaat Farmaceutische en Biologische Technieken, KAHO Sint-Lieven, Departement Gent. Begeleider: Bart Cottyn Trossaert L. Studie van diverse technieken voor het vermeerderen van het graanwortellesieaaltje Pratylenchus crenatus. Graduaat Cel- en genbiotechnologie, KATHO departement HIVB, Roeselare. Begeleider: Nicole Viaene Van Hoorde R. Mogelijkheden van klaver en luzerne op het moderne melkveebedrijf. Bachelor agro- en biotechnologie, Hogeschool Gent. Begeleider: Alex De Vlieger Master Ahuir J. S. Genetic diversity and phylogeny of Malus populations from Central Asia. Master thesis made in the framework of the Socrates programme. Promotoren: Erik Van Bockstaele, Isabel Roldán-Ruiz & Els Coart Baidya S. Interaction between Pratylenchus penetrans and P. crenatus in maize. Afstudeerwerk Master of Science in Nematology, Universiteit Gent. Begeleider: Nicole Viaene Boone W. Ondersteunende technieken bij de veredeling van sierteeltgewassen. Hogeschool Gent, thesis. Begeleider: Katrijn Van Laere Debeer L. Studie van de genetische diversiteit in het genus Crataegus (Meidoorn): interspecifieke hybridisatie en herkomstanalyse. Universiteit Gent, Bioingenieurswetenschappen, Cel- en genbiotechnologie. Promotoren: Erik Van Bockstaele & Els Coart De Winne N. Interspecifieke hybridisatie bij sierteeltgewassen. HoGent, CTLBME, Ind Ing. Biotechnologie; thesis. Begeleider: Katrijn Van Laere Eyskens K. Veredeling van sierteeltgewassen. KHK-Geel, thesis. Begeleider: Katrijn Van Laere Gobert W. Approche de surveillance du Ralstonia solanacearum dans le district de Beibei, province de ChongQing en Chine. Ingénieur Industriel en Agriculture, Haute Ecole Provinciale de Charleroi. Begeleider: Johan Van Vaerenbergh 117 Kakaire S. Species diversity of root-knot nematodes (Meloidogyne spp.), infecting tomato in Uganda. PINC, Universiteit Gent, Faculteit Wetenschappen. Begeleiders: Maurice Moens en Waeyenberge Lieven Kennis P. Detectie van Phytophthora citricola in water m.b.v. filtratie. Hogeschool Gent. Begeleider: Kris Van Poucke Leurs J. Teeltmethode en rassenonderzoek biologische snijmaïs. Industrieel ingenieur in de Landbouw en de Biotechnologie, optie landbouw, Katholieke Hogeschool Kempen, Dep. Ind. Ing. Biotechniek. Begeleiders: Johan Van Waes en Koen Willekens Merckx K. Invloed van soortkruisingen op de vorming van ongereduceerde gameten bij begonia. Hogeschool Gent, thesis. Begeleider: Angelo Dewitte 7. Communication 7.1 Press contacts and visits of Belgian and foreign delegations 3rd of February: Interview “Landbouw kan niet zonder onderzoek” (Erik Van Bockstaele). Boer en Tuinder nr.5 Saiz Forés, J. M. Development of SSR-markers useful for genetic analysis in Leek (Allium ampeloprasum L. var. porrum). Master thesis made in the framework of the Socrates programme. Promotoren: Erik Van Bockstaele, Isabel Roldán-Ruiz & Hilde Muylle 9th of February: Landbouwpers Boer & Tuinder bij ILVO-T&V voor “Ten dienste van de melkveehouderij”. Promotieartikel over de werking van TAD Hoevezuivel Scariot V. Università di Torino (Italië). Begeleider: Ellen De Keyser 10th of February: Canadese missie. International Scientific Cooperation Bureau for Agriculture and Agrifood Vandendriessche Y. Interspecifieke hybridisatie. HoGent CTL, thesis. Begeleider: Katrijn Van Laere Vermeir N. Overerving en chemische analyse van de geur bij Begonia. Universiteit Gent, Bio-ingenieurswetenschappen. Promotoren: Erik Van Bockstaele, Herman Van Langenhove en Johan Van Huylenbroeck Wiseborn D. B. Survey of plant-parasitic nematodes in turfgrass and sand dunes in Belgium with special emphasis on Meloidoyne spp. Afstudeerwerk Master of Science in Nematology, Universiteit Gent. Begeleider: Nicole Viaene Technology & Food 1st of March: Interview “Zonder landbouwonderzoek geen innovatie” (Erik Van Bockstaele). VILT maart 3rd of March: Kennis- en adviescentrum ‘Spuittechniek’ beloond in Proeftuinnieuws. 5:38 24th of March: Landbouwpers Landbouwleven bij ILVO-T&V-Agrotechniek “Onderzoek naar drift en driftreducerende technieken” 25th of March: “Mosselcrisis - Zorgen om een Zeeuwse industrie” (Daan Delbare). De Morgen, p 22. Bachelor 7th of April: Bezoek Chinese delegatie, ILVO-T&V Melle Bekaert W. Karakterisering van het toxiciteitsprofiel van aërobe sporenvormende stammen uit melk. Gegradueerde/Bachelor in Chemie, optie Biochemie, KaHo Sint-Lieven, Gent. Begeleiders: Marc Heyndrickx en Valerie De Jonghe 2nd of May: Landbouwpers Landbouw & Techniek bij ILVO-T&V voor artikel “Focus op de verschillende TAD-projecten van het ILVO” Boussery K. Invloed van de melkstal op de kostprijs van melkveestallen. Gegradueerde/Bachelor in Landbouw en Biotechniek, KHK Geel. Begeleider: Katrien Boussery Depraetere L. Ontwikkeling van een vloeistofchromatografische-massaspectrometrische methode voor het opsporen van fluoroquinolones in melk. Gegradueerde/Bachelor in Voedings- en dieetkunde, Erasmushogeschool Brussel, Campus Jette. Begeleider: Els Daeseleire De Ridder K. Detectie en kwantificatie van genetisch gewijzigde organismen: enkele screeningstechnieken. Gegradueerde/Bachelor in Laboratorium- en Voedingstechnologie, optie Farmaceutische en Biologische Technieken, stage 2de jaar. KaHo Sint-Lieven, Sint-Niklaas. Begeleider: Isabel Taverniers 4th of May: Landbouwpers Boer & Tuinder bij ILVO-T&V voor artikel “Sectorgids Hoevezuivel” in het kader van de TAD Hoevezuivel 10th of May: Bezoek H.E. Dato’ Seri Mohd Shariff Omar, Deputy Minister of Agriculture and Agro-Based Industry Malaysia en afgevaardigden op ILVO-Visserij, Oostende. Reportage Boer en Buiten 11th of May: Bezoek studiedienst Boerenbond 11th of May: Veeteelt, interview, Heraanleg van sleufsilo’s, Veeteeltvlees 12th of May: Persconferentie Voorstelling ILVO - Merelbeke 14th of May: “Noordzeetong komt aan land” (Kris Cooreman). Het Nieuwsblad, p 1 Prieels V. De opsporing van remstoffen in de melk door middel van de Copan Milk Test. Gegradueerde/Bachelor in Voedings- en dieetkunde, Erasmushogeschool Brussel, Campus Jette. Begeleider: Wim Reybroeck 14th of May: “Je proeft het verschil niet” (Kris Cooreman). Het Nieuwsblad, p 6 Master 14th of May: “Noordzeetong komt aan land” (Kris Cooreman). De Tijd Danneels B. Gezondheidsindicatoren van het dierenwelzijn van legkippen in verschillende huisvestingsystemen. Industrieel Ingenieur in Landbouw en Biotechnologie, optie Landbouw, Hogeschool Gent. Begeleiders: Bart Sonck en Bastiaan Rodenburg 14th of May: “ILVO brengt Noordzeetong aan land” (Kris Cooreman). VILT Dekeyser D. Bepaling van het effect van luchtondersteuning op de eigenschappen van spuitdruppels. Industrieel ingenieur, Hogeschool Gent. Begeleider: David Nuyttens 15th of May: “Noordzeetong komt aan land” (Kris Cooreman). Metro, p 3 Dezeure L. Het gebruik van regenwater als drinkwater in de melkveehouderij. Industrieel Ingenieur in Landbouw en Biotechnologie, optie Landbouw, Hogeschool Gent. Begeleider: Bart Sonck García García Maria Del Carmen. The effect of sun shading and energy saving screens on radiation. M.Sc. made in the framework of the Socrates programme. Faculty of Bioscience Engineering, Ghent University. Promotoren: Reinhart Verschoore en Willy Dierickx Smedts A. Moleculaire typering van Listeria monocytogenes isolaten van diverse oorsprong. Licentiaat/Master in de Biotechnologie, UGent. Begeleiders: Els Van Coillie en Hadewig Werbrouck Van Ham R. Het verband tussen mechanische schade bij witloofwortels en het optreden van Phoma exigua var. exigua tijdens de forcerie. Industrieel Ingenieur in Landbouw en Biotechnologie, optie Landbouw, Hogeschool Gent. Begeleiders: Bart Sonck , Jürgen Vangeyte en Stijn Windey 14th of May: “Vlaanderen gaat Noordzeetong kweken” (Kris Cooreman). VRTNieuws 16th of May: “Vlaamse primeur met aan land gekweekte zeetong” (Kris Cooreman). Het Nieuwsblad 16th of May : “Des soles élevées sur terre“ (Kris Cooreman). Le Soir 19th of May: “Oostendse tongkwekerij op komst” (Kris Cooreman). De Zeewacht, p 9. 1st of June: Interview “ILVO van start. Onderzoek bundelt krachten” (Erik Van Bockstaele). Landgenoten zomer 2006 12th of June: Bezoek Hoofdbestuur Boerenbond met voorzitter Noël Devisch – ILVO-T&V 16th of June: “Eten we volgend jaar Belgische mosselen?”. Boer en Tuinder nr.24 16th of June: “Maatje is een paljas” (Daan Delbare). Het Belang van Limburg, p 39 16th of June: “Belgische Mosselkweek” (Daan Delbare). Interview Focus regionale TV 19th of June : Bezoek Beleidsdomein Landbouw & Visserij (Vlaams viceministerpresident en Vlaams minister van Economie, Ondernemen, Innovatie, Wetenschap en Buitenlandse Handelminister Fientje Moerman) 118 22nd of June: Persconferentie met bezoek aan veldproeven met aardappelen onder conserveringslandbouw ”Spectaculaire verschillen in beginontwikkeling” organisatie van ILVO-Plant-TO. 23rd of June: “Focus op het ILVO en de technologische adviseerdiensten” in Landbouw & Techniek 12, p. 35-38 14th of July: “Boer wordt viskweker” (Daan Delbare). Het Nieuwsblad, p. 1 14th of July: “Kreeft vervangt varken” (Daan Delbare). Het Nieuwsblad, p. 1011 15th of July: “Boeren staan niet te watertanden voor aquacultuur” (Daan Delbare) VILT 7.2 Activities, workshops and courses organised by or in cooperation with ILVO Animal Sciences 9th of September: Stand ILVO-Visserij op de Oostendse Visserijfeesten en Havendag 23rd of November: Depestele J., Polet H., Vanderperren E., Stouten H., Van Craeynest K. (2006) Duurzame vis op ‘t menu. “De Zee op de korrel”, vorming voor zeeanimatoren over duurzame vis. Oostende, Provinciaal Ankerpunt Kust (B). 22nd of September: Formele samenwerking ILVO-Visserij en VLIZ bekrachtigd: Twee toonaangevende Vlaamse zee-instituten bundelen krachten (Kris Cooreman) 15th of Juni: Infonamiddag van ILVO-Dier Melle “Actuele onderzoeksresultaten bij éénmagigen”. 25th of September: “Instituten VLIZ en ILVO werken voortaan samen rond visserij” (Kris Cooreman). Laatste Nieuws-Regio 27th of March and 5th of December: Studienamiddagen van de World Poultry Science Association (WPSA) op ILVO-Dier Melle. 1st of October: Interview “ILVO zet Merelbeke op de wereldkaart!” (Erik Van Bockstaele). Infomagazine Merelbeke oktober 2006 27th December: Workshop “Duurzame en ecologische visserij”. Oostende (B). Ingericht door ILVO-Visserij, Sectie Technisch Visserijonderzoek. 12th of October: AVS: Opname voor het programma “Boer en Buiten”. “Rassenonderzoek” bij ILVO-Plant-TO 18th of October: “Sportvisserij weegt zwaar op de visbestanden” (Frank Redant). VRTNieuws.net 19th of October: “ILVO pleit voor meer beperkingen op sportvisserij” (Frank Redant). VILT 19th of October: “Sportvissers wegen zwaar door op visbestand” (Frank Redant). Metro 19th of October: “Sportvisserij heeft zware impact op visbestand” (Frank Redant). www.biteback.be 31st of October: Bezoek kabinet Beleidsdomein Landbouw & Visserij (Vlaams Minister-President Yves Leterme) 1st of November: “Sportvisserij in België”. In: Sportvisserij weegt op het mariene visbestand: de zware impact van een hobby (Frank Redant). EOS, November 2006, p. 41. 6th of November: Pers KVLV bij ILVO-T&V voor tijdschrift “Vrouwen met vaart” Social Sciences 7th of June: Workshop “Biotechnology: Economic challenges and society’s perceptions”; organisation Belgian Association of Agricultural Economists, in collaboration with the Federal Public Service Economy, Small and Medium Enterprises, Self-employed and Energy and ILVO – Social Sciences Unit 4th of July: Discussion platform “Bottlenecks in research for organic agriculture”, within the framework of the project “Opportunities for optimisation and structuring of organic farming research in Flanders”, ILVO – Social Sciences Unit 26th of September: Discussion platform “Opportunities for optimising knowledge exchange within the organic sector”, within the framework of the project “Opportunities for optimisation and structuring of organic farming research in Flanders”, ILVO – Social Sciences Unit. Plant Sciences De Keyser E., Leus L., Audenaert K., Heungens K., Malengier M., Muylle H. (2006) Resistentieveredeling, cursus, 62, ILVO – Plant-GV, Melle (B). 10th of November: “Straks geen vis meer in zee?” (Wim Demaré). Zeewacht De Riek J., Lamote V., Eeckhaut T., De Keyser E., Van Laere K., Van Huylenbroeck J. (2006) Erfelijkheidsleer in de praktijk. Cursus, 185 p. 17th of November: “Belgian catch tops commercial fishermen” (Frank Redant). Fishing News Van Huylenbroeck J. (2006) Erfelijkheidsleer in de praktijk. Deel veredelingstechnieken. Cursus, Melle (B). 24th of November: Landbouwpers Landbouw & Techniek bij ILVO-T&V voor “Focus op waardecreatie in de zuivelsector” in het kader van de TAD Hoevezuivel 11th of January: seminarie “Landbouw heeft een toekomst: ook met nieuwe MAP en reductieplan bestrijdingsmiddelen! Teeltgericht bodembeheer met levende input: compost en compostthee” te Gent; organisatie van FarmCOMPOST ILVO-Plant-TO. 1st of December: “Expertise is klaar voor de praktijk”. In: “Het begon met een aquarium in ons tuinhuis” (Daan Delbare). Landgenoten, Winter 2006, p. 13. 6th of December: “Sportvisserij” (Frank Redant). Eén – Koppen 12th of January: studienamiddag “Toepassing van compostthee in teelten op hydrocultuur” op ILVO-Plant-GV te Melle; organisatie van FarmCOMPOST ILVO-Plant-TO. 8th of December: “Sportvisserij” (Frank Redant). VRT Radio - Voor de dag 21st - 23rd of January: Agro-Expo Vlaanderen, Roeselare. Deelname ILVOPlant-TO. 15th of December: Persconferentie “Preventagri Nouveau” en aankondiging van de wedstrijd rond veiligheid voor landbouwscholen, samenwerking met FOD Arbeid en Tewerkstelling, in Crown Palace Hotel te Brussel 24th of January - 9th of February (op dinsdag en donderdag): Erfelijkheidsleer in de praktijk (Sietinet, ILVO, Azalea Innovatiefonds) 18th of December: Voorstelling beschrijvende en aanbevelende rassenlijst voor voedergewassen en bodembedekkers – SEMZABEL – ILVO-Directie en Plant, T&V 7th of February: Workshop GMO (ILVO-Plant) 20th of December : “Quotabeleid“ (Frank Redant). L’Echo de la Bourse 27th of April: studiedag KVIV rond energiegewassen 20th of December: “Quotabeleid“ (Frank Redant). Het Belang van Limburg 25th of June: opendag ‘Inspiratie voor Innovatie – 50 jaar POVLT’ RumbekeBeitem. Deelname ILVO-Plant-TO. 21st of December: “Hoe gezond is zalm (nog)?” (Daan Delbare). Het Laatste Nieuws, p. 14. 21st of December: Uitvoering van rookproeven in Boer & Buiten op AVS 22nd of December: “Quotabeleid” (Frank Redant). VRT Radio - Lopende Zaken, Kanaal Z – Journaal, Canvas – Terzake 23rd of December: “Visserijproblemen en quotabeleid” (Frank Redant). De Tijd Landbouwleven 20: 4. Uitreiking getuigschriften Gekwalificeerd Technicus Spuitmachines 20th of March: scientist@work (ILVO-Plant) 29th - 30th of June: Workshop Generose, Genetic evaluation of European rose resources for conservation and horticultural use, (ILVO-Plant) September: Opleiding medewerkers FAVV. Theoretische en practische sessies Casteels Hans, Heungens Kurt, Moens Maurice, Van Vaerenbergh Johan, Viaene Nicole. 19th of September: Workshop ‘Karakterisering van autochtone bomen en struiken in Vlaanderen’ (ILVO-Plant, Sietinet, INBO, Van Hulle B&C Boomkwekerijen) 27th - 29th of September: Grasp-workshop ‘focus on data-analysis’ (ILVOPlant-GV) 119 7th and 14th of November: Cursus resistentieveredeling (Azalea Innovatiefonds, Sietinet, ILVO, Ugent) 30th of November and 1st of December: Workshop on data analysis (Grasp, ILVO-Plant-GV) 18th of December: Voorstelling van de “Belgische beschrijvende en aanbevelende rassenlijst voor voedergewassen en groenbedekkers 2007” op ILVO-Directie met medewerking van ILVO-Plant-TO Technology & Food 24th of November (Marloie): workshop “Veterinary Drugs Residue Analysis and Farm to Fork Approach”, Food Safety Cluster; met medewerking van ILVOT&V. 5th of January (Merelbeke): Workshop statistiek, spreker Prof. Dr. Dirk Berkvens, Tropisch Instituut Antwerpen. 28th of November: infovergadering wetenschappelijke begeleiding industrie vlaanderen; organisatie ILVO-T&V. 11th of January (Melle): Ontbijtsessie “Microbiologische voedselveiligheid en bewaartechnieken”, Food2Know (UGent). 30th of November: Themadag T&V voor Departement en Agentschap Landbouw en Visserij. 27th of January (Tongeren), 2nd of February (Gooik), 23rd of February (Melle), 8th of March (Torhout), 15th of March (Poperinge), 6th of November (Huldenberg), 7th of November (Lichtaart), 8th of November (Sleidinge) en 14th of November (Zele): Testen voor de opsporing van antibiotica op de hoeve. Demosessie voor landbouwers en studenten van het landbouwonderwijs in het kader van de TAD Hoevezuivel; organisatie ILVO-T&V in samenwerking met de landbouworganisaties. 7th of February (Merelbeke): Workshop “GMOs”, Food Safety Cluster; organisatie ILVO-T&V. 9th of February (Melle): Autocontrole paardenmelkpoeder. Infosessie voor paardenmelkers die klant zijn van de pilootfabriek; organisatie ILVO-T&V. 24th of April (Gent): Opleidingsonderdeel Seminaries, Bio-ingenieur Landbouwkunde “Wetenschappelijk onderzoek en kwaliteitsbeleid in landbouw” (UGent). 16th of May (Antwerpen): International Satellite Congress, Food Safety Cluster; organisatie ILVO-T&V en UGent. 16th of May (Veulen): “Huisvesting vleesvee”. Studienamiddag in samenwerking met ADLO. 16th of May (Merelbeke) and 18th of May (Ciney): Studiedag Control; in samenwerking met IKM Vlaanderen, VRV, Boerenbond, QFL Wallonie, AWE. 23rd of May (Lotenhulle): ”Huisvesting vleesvee”, studienamiddag in samenwerking met ADLO. 30th of May (Bocholt): Demonstratie stalknechten op het rundveebedrijf. Demonstratienamiddag in kader van TAD Agromech in samenwerking met landbouwschool Bocholt. 2nd and 6th of June (Gent & Melle): Workshop ‘Quality control of honey. Honey and legislation.’ in samenwerking met het Informatiecentrum voor Bijenteelt (UGent) in het kader van de cursus ‘Beekeeping for Poverty alleviation’. 13th of June (Merelbeke): “Koolzaadolie: van boer tot consument”. Studienamiddag in het kader van ALT-demo KOBRA, in samenwerking met LTCW. 7th of August (Melle): Activities of ILVO with emphasis on dairy technology and bacterial food safety, IPBO summer course; met medewerking van ILVO-T&V. 7th of September (Gent): studiedag “Alternatieven voor voederantibiotica: zoötechnie en voedselveiligheid verenigd?”; met medewerking van ILVO-T&V. 9th - 17th of September: Accenta, Gent. Deelname van ILVO-T&V, Productkwaliteit en –innovatie. 14th and 15th of September (Gent): ‘Eleventh Conference on Food Microbiology’; met medewerking van ILVO-T&V. 26th of September (Aalter): “Wassers & biobedden in het kader van ammoniakemissiereductie”, studiedag in samenwerking met ADLO 28th of September (Poperinge): “Wassers & biobedden in het kader van ammoniak-emissiereductie”, studiedag in samenwerking met ADLO. 24th - 25th of October (Montoldre, France): The 2sd International Symposium on Centrifugal Fertiliser Spreading, ILVO is lid van scientific committee. 7th of November (Zwevegem): “Huisvesting vleesvee”, studienamiddag in samenwerking met ADLO. 14th of November (Beervelde): “Huisvesting vleesvee”, studienamiddag in samenwerking met ADLO. 21st of November (Melle): Workshop ‘Quality control of honey’ in samenwerking met Wageningen Expertise Centre for Chain and Network in het kader van de ‘Supply Chain Course Bosnia and Herzegovina’. 21st of November, 5th of December, 7th of December, 14th of December, 19th of December: Paratuberculose, in het kader van de TAD Hoevezuivel; organisatie ILVO-T&V in samenwerking met Dierengezondheidszorg Vlaanderen en Boerenbond. 120 121 122
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