Marine Biodiversity of Raja Ampat Islands

i
PEER Science Annual Report
1st June 2012- 30th April 2013
Marine Biodiversity of Raja Ampat Islands:
the ARMS, Morphology, and Genetic approaches for
inventorying and monitoring patterns of marine
biodiversity
1
Project Title:
Marine Biodiversity of Raja Ampat Islands: the ARMS, Morphology, and Genetic
approaches for inventorying and monitoring patterns of marine biodiversity
Principal Investigator(s):
Institution(s):
Abdul Hamid Toha
State University of Papua
Reporting Period: June 2012-April 2013
Summary of Activities:
Overview: Outcomes from this project include establishing the Genetics Laboratory of
UNIPA as a site for a field station, the digitization of voucher specimens, a project web,
and for databases of marine biodiversity. Other outcomes from this research also
include a manuscript of Conservation Genetics and a newsletter.
During the first period, research and laboratory equipment have been prepared
and some field activities have been conducted. In Educational activities, we already
held four workshops or training from June 1, 2012 through April 30, 2013 include the
following: 1) Biodiversity, Molecular Ecology and Genetic Conservation, 2) Inventorying
Marine Invertebrates and Microbes of Raja Ampat Islands, 3) Molecular Genetic Basics,
and 4) Conservation Genetics. These courses had a total 129 participants from some
institutions in Manokwari. We also support student’s field work practice in Raja Ampat
along two months.
In Research, we already collected invertebrate specimens included water
sample in order to collect bacteria samples. In total, there are 33 species invertebrate
that identified in this period. Among them, two species has already analyzed and in
preparation of writing article for publication. The researches affair had a total 23
participants from State University of Papua and Brawijaya University. Data base of
these specimens have already prepared and will be published in our website. Research
related to the one species of invertebrate which was T. gratilla from Raja Ampat has
been done by graduate student in Brawjaya University. We also got a new research
grant from State Ministry of Research and Technology of the Republic of Indonesia.
Beside that we are part of a consortium who won new NSF PIRE award to continue our
collaborative biodiversity research in whole Indonesia.
In Publications, we have already published 11 monthly Newsletters
“Konservasi Biodiversitas Raja4” or “Raja Ampat Biodiversity Conservation” from June
2012 through April 2013. We also have a website in www.ibcraja4.org to socialize this
project and other things related by conservations, biodiversity, Raja Ampat, laboratory
and field methods. Book manuscript of “Diversity and Conservation of Sea Urchin” was
in press for publication. Two articles have published in national journal. Beside that we
were also preparing two articles else to published. We also socialized PEER sciences
to all lecturers in UNIPA. The publications affair had a total 17 participants from State
University of Papua and Brawijaya University.
2
Events:
We held four workshops or training from June 1, 2012 through April 30, 2013.
They are 1) training about Biodiversity, Molecular Ecology and Genetic Conservation on
September, 2012 taught by Prof. Sutiman B. Sumitro, Luchman Hakim, Ph.D and
Hamid Toha, 2) workshop the Field Work Practice for Inventorying Marine Invertebrates
and Microbes of Raja Ampat Islands on December, 2012 taught by Hamid Toha and M.
Dailami, 3) training Molecular Genetic Basics (DNA technology for Conservation) taught
by Hamid Toha and Kuncoro, and 4) general lectures of Conservation Genetics on
April, 2013 taught by Hamid Toha. These courses had a total 129 participants from
different institutions in and outside UNIPA. We also support undergraduate student’s
field work practice in Raja Ampat along two months. This field work had a total 10
participants from UNIPA.
As a way of research building capacity for young faculty and students, our
project had facilitated the faculty and students to join in research at Raja Ampat. Six
participants are now conducting research with focus on invertebrates of Raja Ampat. In
addition, we support undergraduate, master and doctoral students in research for their
thesis and dissertation. These events had a total 7 participants from UNIPA and UB.
Below is the detail of the workshops.
Number of
workshops
4
Date of
workshop,
conference, or
visit
April 11-12,
2013
Number of
conferences
0
Number of
exchange
visits with
U.S. partners
0
Location of
workshop,
conference,
or visit
UNIPA,
Manokwari
UNIPA,
Manokwari
UNIPA and
Brawijaya
University
Title of workshop or
conference or name of
visitor
Dec, 2012-Peb
2013
Raja Ampat
Dec 2012March 2013
UNIPA
December 1920, 2012
UNIPA,
Manokwari
September 1012, 2012
UNIPA,
Manokwari
April 11, 2013
March- July
2013
Number of
female
participants
Number of
male
participants
9
8
28
31
Support in research for
thesis and dissertation
3
4
Field Work Practice
8
2
5
1
9
13
15
16
Training of Molecular
Genetic Basics
General Lecture of
Conservation Genetics
Call for proposal
(Capacity building in
research)
Inventory of Raja Ampat’s
Invertebrates Biodiversity;
Work Field Practice
Workshop
Biodiversity, Molecular
Ecology and Genetic
Conservation Workshop
3
Educational Impacts:
Researchers, students, and faculty from different backgrounds and institutions in
and outside UNIPA get benefit from our project through three kinds of the activities
(training and teaching, research, and publication). Educational curricula have been
developed especially for Genetic subject in Fishery Department. We also add a
practicum in existing subject course in Molecular Biology and Genetics for multi
faculties in UNIPA. Formal educational activities included 4 new courses.
We also had facilitated the faculty and student to join research as a way of
research building capacity for young faculty and students in UNIPA and UB. Our project
also supported undergraduate, master and doctoral students in research for their thesis
and dissertation.
Educational Status
Undergraduate (BS)
Masters Student (MS or M Phil)
Doctoral Student (PhD)
Postdoctoral scholars
Other junior collaborators
Laboratory Technicians
Number of
Females
5
0
1
0
5
2
Number of
Males
10
1
0
0
1
3
Other participant involved in our project was detailed in Supplementary information.
Infrastructure Development:
In this period, we got a new research grant from State Ministry of Research and
Technology of the Republic of Indonesia. The title of research is ”Seleksi dan
Domestikasi Kandidat Indukan Udang Galah Macrobrachium rosenbergii (de
Man, 1879) asal Papua berdasarkan Marka Genetik untuk Pengembangan
Indukan Baru”. This research began on January to December 2013. The team of
this research is Robi Binur (Head, UNIPA), Adi Pancoro (Researcher, Bandung
Institute of Technology) and Abdul Hamid Toha (Researcher, UNIPA). Beside
that we are part of a consortium who won new NSF PIRE award to continue our
collaborative biodiversity research in whole Indonesia. The proposal “PIRE:
Assembly of Marine Biodiversity Along Geographic and Anthropogenic Stress
Gradients” was funded by the U.S. National Science Foundation. The grant
funding period will commence on January 1, 2013. Duration program is five
years from 2013-2015.
In this period we have already bought a camera for documentation, laptop for
server database and website. We also purchased new micropipettes for training
and teaching. We used these equipments when the training was running. The
equipments also used in research of student and faculty who wants to finishing
their study and conduct individual research.
4
Below is a list the number of new grants received as a result of PEER Science funding.
International
Number of research grants
received
Number of patent
applications submitted
Local (in PI’s country)
1
1
0
0
We have not yet a patent applications until now. However, we are publishing our book
manuscript that will get a copyright. The manuscript was not fully funded by PEER but
PEER have already made this happen as soon as possible.
Publications:
We already published a monthly newsletter. Until now, we have published eleven
editions of newsletters from June 2012-April 2013. For socializing of our project with the
program, we also launched a website at www.ibcraja4.org (look at Supplementary
Information). We are also preparing manuscript three books and three articles that
related to our project but not include in our proposal before.
Books
1. Toha, A.H.A. Molecular Genetics (Revision Edition). For submission to AlfaBeta
Publisher, Bandung. (in Bahasa)
2. Toha, A.H.A., Sumitro, S.B., Hakim, L., Widodo. “Diversity and Conservation of
Sea Urchin”. For submission to publisher. (in review)
3. Toha, A.H.A., Mahardika, A.P.W. “Sea Urchin Tripneustes gratilla”. For
submission to publisher. (in Bahasa)
Articles
4. Toha, A.H.A, Sumitro, S.B., Hakim, L., Widodo. Genetic connectivity of sea urchin
Tripneustes gratilla in Cenderawasih Bay, Papua-Indonesia. For submission to
International journal (in review).
5. Toha, A.H.A., Sumitro, S.B. L. Hakim., Widodo. 2012. Kondisi habitat bulu babi
Tripneustes gratilla (Linnaeus, 1758). Berk. Penel. Hayati 17 (2): 139-145.
6. Toha, A.H.A., Permana, A., Sumitro, S.B. L. Hakim., Widodo. 2012. Penentuan
jenis kelamin bulu babi Tripneustes gratilla secara morfologi. Berk. Penel. Hayati
17 (2): 211-215.
Type of Technical
Publication
Journal articles
Proceedings
Technical reports
Books
Book chapters
Book editor
International
Publications
1
1
Local
publications
(in PEER PI’s
country only)
2
Number of joint
publications
with U.S. PEER
partner
3
We are also preparing one paper about spiny lobster Panulirus versicolor for national
journal entitled: Genetic structure of Lobster Panulirus versicolor in Raja Ampat waters.
5
Technical Research Presentations:
We attended four conferences and seminars of national or international in June
to September 2012. First, we attended a national seminar (4th Biodiversity National
Seminar) in Airlangga University at Surabaya, East Java on September 15, 2012. We
presented a paper “Genetic diversity of Sea Urchin Tripneustes gratilla (Linnaeus 1758)
in Papua”. Second, we presented a paper “Genetic diversity pattern of Tripneustes
gratilla in Indonesia”. The paper was presented at Indonesian Biodiversity Research
Center Synthesis Meetng in Udayana University at Denpasar on Augusts 7, 2012.
Third, we presented Marine protected areas network, a mtDNA studies for conservation
planning: A case study of Tripneustes gratilla. This paper was presented at the 3rd
International conference on global resources conservation in Brawijaya University at
Malang on July 7, 2012. Lastly, we present Comparative genetic diversity pattern of
Tripneustes gratilla in the Indonesian Archipelago and Indo-Pacific at Coral Triangle
Partnerships for International Research and Education synthesis meeting in Manila
(Philippines) on June 16, 2012.
I also presented a paper “Education and Research for Biodiversity in Raja
Ampat and Indonesia”. Short Review: PEER, PIRE, IBRC, NESCent, etc. in State
University of Papua on April 12, 2013. This seminar was done for monitoring and
evaluation our project for full year.
Number of technical
research presentations
made
5
Linkages and Potential Development Impacts:
We attended a PEER Financial Workshop that held by USAID in Jakarta on
November 20-21, 2012. In this workshop, we shared our activities and financial
management with other institutions that invited by USAID. We also get important things
from this workshop in financial management.
When we held the training in UNIPA, we have any linkages developed with
different backgrounds and institutions outside UNIPA. They are from government
agencies in our country (Center of Research and Development of Agricultural Papua
Barat, Center of Research and Development of Forestry Province Papua Barat,
National Park of Teluk Cenderawasih agency, and Ministry of Marine and Fishery
Province Papua Barat), from private companies (STKIP Muhammadiyah, local private
university in Manokwari), and Community groups or nongovernmental organizations
(Conservation International-Indonesia). We intend to expand this work in the future
especially in working of biodiversity etc.
Our project was visited by IBTC team (International Business and Technical
Consultant Inc.) for monitoring and evaluating the Indonesian Biodiversity Research
Center (IBRC) on November 2-3, 2012. Besides that team of Directorate of Bilateral
Foreign Funding, National Development Planning Agency (BAPPENAS) of Republic of
Indonesia was also visit us on December 10, 2012. Although they weren’t coming solely
for PEER project, however, they also got information about PEER project from us.
These important for us and the project to anticipate what will happen in the future.
6
This project was also result in significant benefits for national co-operation and
collaboration with Brawijaya University, as an state institution poised to be a resource
for scientific collaboration in Indonesia. This partnership creates a strong platform for
research and a direct mechanism into actionable conservation. We also have started to
work together with Warren Wilson College, US, in partnership on educational and
research at Raja Ampat.
Organization
Yes
USAID
Yes
Government agencies in your country
Yes
Community groups or nongovernmental organizations
Yes
Private companies
Yes
No
Problems Encountered:
We ordered ARMS equipments from US then ARMS shipped from Jakarta to
Sorong (West Papua) since 2012. However, the ARMS deployment in Raja Ampat
waters is not yet running until now. The ARMS will be deployed at the Raja Ampat
waters adjust with US our partner’s schedule on summer. Because of ARMS will keep
in a full year in bottom of the sea, we think to get the results of ARMS will need more
time. This condition will make any problems we may have encountered in making
progress on our project objectives.
To resolve the problem, we have to deploy the ARMS in this summer with or
without our U.S. partners. We also tried to conduct research with other method (without
ARMS). By this method we have already collected many specimens from Raja Ampat. I
think we have to run the same ways again in the next period. Although ARMS results
will be taken, the data of this method will still function and could be compared with
ARMS products.
We also have difficulties related by processing ISSN for our monthly newsletter.
Until now, our monthly newsletters have no ISSN. We also have difficulties related by
publishing our books. Recently, UNIPA press (UNIPA publisher) did not work again and
we could not get a support letter for publishing ISSN and ISBN. Therefore, we interest
to set up a publisher for supporting our publications. The publisher will function and
keep on both in the short term and in the long term.
Plans for Activities During the Coming Year:
We intend to deploy ARMS at Raja Ampat waters adjust with our US partner’s
schedule on this summer. We also intend to conduct lab researches with selected
young researcher from our invitation in call for proposal. In addition, we will publish
activities and some of the result from field work practice in posters form, and we will
upload in website and newsletter. We also planned to publish our two books and three
articles. We want to make a copy of our published book and available free for all on our
website.
We intend to held field work with the US students and faculty in Raja Ampat on
May, 2013. We will work together with lecturers of Brawijaya University, professor from
7
Warren Wilson College who will run a field trip in Raja Ampat. Our team will conduct
research about conservation and will collect specimen of invertebrate. Some travel will
run in the second year of this project.
The project is now preparing the second year molecular genetics course, there
are 35 participants from different institutions in and outside UNIPA anticipated to join
2013. Furthermore, in the second year of the project we increasing both the number of
courses taught and the number of students involved. We also will expand our research
projects by partnering with various local and international institutions and project
personnel that have submit their proposal through the national and international
competitions.
Additional Comments:
We intend to revise our budgeting to maximize our project workings. This is including
revise in other direct costs, for instance, publication costs, workshops and conferences,
and digitizing collection. We have no allocate budget for these item before. The fund
will be taken from equipment’s fund that not fully used.
Supplementary Information:
Below is supplementary information of our project.
8
TRAINING AND TEACHING
1. Training Workshop: Biodiversity, Molecular Ecology and Genetic
Conservation
Agenda
Day 1
Time
09:00 – 09:10
09:10 – 09:30
09:30 – 09:45
09:45 – 10:00
10:00 – 11:15
Activities
Welcome
Review of MB-RAI
Speech
Break
Molecular Ecology 1
11:15 – 12:30
Biodiversity 1
12:30 – 13:15
13:15 – 14:00
14:00 – 15:00
15:00 – 15:15
15:15 – 16:00
Break
Extraction Method of DNA
Amplification Method (PCR)
Break
GenBank
Presenter
Ketua panitia
A.Hamid Toha
Rektor
Prof. Sutiman B. Sumitro, SU,
D.Sc (UB)
Luchman Hakim, M.AgrSc,
Ph.D (UB)
Moderator
Committee
Committee
Committee
Hamid
Ema
Hamid Toha
Hamid Toha
Hamid Toha
Day 2
Time
09:00 – 09:30
09:30 – 09:45
09:45 – 10:00
10:00 – 11:15
Activities
MB-RAI website
Review of ctPIRE
Break
Biodiversity 2
11:15 – 12:30
Molecular Ecology 2
12:30 – 13:15
13:15 – 14:00
Break
Agarose Electrophoresis
Method
Clean up amplification
product method
Break
Cycle sequencing
14:00 – 15:00
15:00 – 15:15
15:15 – 16:00
Presenter
Jeni, M.Si/M. Dailami
A.Hamid Toha
Luchman Hakim, M.AgrSc,
Ph.D (UB)
Prof. Sutiman B. Sumitro, SU,
D.Sc (UB)
Moderator
Panitia
Panitia
Panitia
Ema
Sabarita
Hamid Toha
Hamid Toha
Hamid Toha
Day 3
Time
09:00 – 09:45
Activities
Aricle writing techniques
09:45 – 10:00
10:00 – 11:00
Break
Conservation Genetics
11:00 – 12:00
Genetic analysis
12:00 – 13:15
13:15 – 14:00
14:00 – 15:00
15:00 – 15:15
15:15 – 16:00
Break
Mega5
DNAsp 5.10
Break
Arlequin
Presenter
Luchman Hakim, M.AgrSc,
Ph.D (UB)
Widodo, M.Si,
Ph.D.Med.Sc/A. Hamid Toha
Widodo, M.Si,
Ph.D.Med.Sc./A. Hamid Toha
Hamid Toha
Hamid Toha
Hamid Toha
Moderator
Committee
Committee
Jeni
Sabarita
9
Training Workshop: Biodiversity, Molecular Ecology and Genetic Conservation
2. Debriefing Field Work Practice for Inventorying Marine Invertebrates and
Microbes of in Raja Ampat Islands.
Agenda
Time
Activities
Titles
Wednesday, Desember 19, 2012
09.00 –
09.30
09.30 - 10.30
10.30 –
11.30
Debriefing and
Introducing
research
program
Opening
Teknik Pengambilan Sampel
Iinvertebrata dan Mikroba untuk Data
Morfologi dan Genetik
Panduan Penulisan Laporan PKL dan
Kriteria Penilaian PKL
Dinamika Sosial Masyarakat Pesisir
dan Etika Penelitian di Masyarakat
11.30 - 12.30
Presenter
Head of Fishery
Departmen, FPPK
A.Hamid A. Toha
Simon P.O.
Leatemia, S.Pi,
M.Si
Nurhani
Widiastuti, S.Pi,
M.Si
Tuesday, Desember 20, 2012
09.00 –
10.00
10.00 –
11.00
Presentation
of Mangrove
group
Presentation
of
Makrozoobent
os & Mollusca
at Mangrove
groups
1. Inventarisasi dan Pemanfaatan
Jenis-Jenis Mangrove oleh
Masyarakat Lokal di Kabupaten
Raja Ampat
2. Komposisi Jenis dan Kerapatan
Mangrove di Kampung Amdui Selat
Sagawin Kabupaten Raja Ampat
3. Inventarisasi jenis-jenis mangrove
di Pulau Kaki Distrik Manokwari
Utara Kabupaten Manokwari
1. Inventarisasi Makrozoobenthos di
Ekosistem Mangrove pada
Kampung Amdui Distrik Selat
Sagawin Kabupaten Raja Ampat
2. Asosiasi moluska pada ekosistem
Mangrove di Kampung Amdui Selat
Sagawin Kabupaten Raja Ampat
3. Asosiasi Gastropoda pada
Sampari Suruan
Harry Silalahi
Maryo B.
Mandacan
Putri Ngilawane
Deasy Yamco
10
4.
1.
2.
11.00 –
12.00
Presentation
of groups of
3.
sea cucumber,
Kima and
Sepuncula
4.
12.00 –
13.00
13.00 –
14.00
14.00 –
15.00
15.00 –
16.00
Ekosistem Mangrove di Kampung
Sawinggrai Distrik Meosmanswar
Kabupaten Raja Ampat
Kelimpahan jenis Bia Kodok
(Polimesoda spp) di Kampung
Amdui Distrik Selat Sagawin
Kabupaten Raja Ampat
Inventarisasi Jenis-Jenis Teripang
Hasil Tangkapan dan Pemanfaatan
oleh Nelayan
Kelimpahan Jenis Teripang di
Kampung Sawinggrai Distrik
Meosmanswar Kabupaten Raja
Ampat
Kelimpahan Jenis Kima
(Tridacnidae) di Kampung Amdui
Selat Sagawin Kabupaten Raja
Ampat
Sebaran dan Pemanfaatan Filum
Sipuncula di Kampung Amdui
Distrik Selat Sagawin Kabupaten
Raja Ampat
David Lomban
Steven Ayomi
Bayu Pranata
Ronald
Hambuako
Absalom Wakum
Rico R. Mailissa
Break
Presentation
of Group of
Social
economy,
aquaculture
and fisihing
Presentation
of group of
seaweed and
Macroalgae
Presentation
of groups of
Mollusc and
fish at
seaweed
1. Analisis Hasil Pendapatan usaha
budidaya ikan lele dumbo dengan
system kolam di Distrik Masni dan
Distrik Prafi
2. Sasi di Kofiau Kabupaten Raja
Ampat
3. Budidaya kerang mutiara (Pinctada
sp) di Kampung Amdui Distrik Selat
Sagawin Kabupaten Raja Ampat
4. Konstruksi Alat Tangkap Jaring
Payang di Kabupaten Manokwari
1. Inventarisasi Jenis-jenis Makroalga
di Kabupaten Raja Ampat
2. Komposisi dan Kelimpahan Jenis
Lamun di Kabupaten Raja Ampat
3. Inventarisasi jenis-jenis lamun di
Pulau Kaki Distrik Manokwari Utara
Kabupaten Manokwari
1. Asosiasi dan Komposisi Ikan pada
Ekosistem Lamun di Distrik Amdui
Kabupaten Raja Ampat
2. Kelimpahan Jenis-Jenis
Gastropoda Dan Bivalvia (Molusca)
pada Ekosistem Lamun di .
Kampung Amdui Distrik Selat
Sagawin Kabupaten Raja Ampat
3. Kelimpahan jenis Gastropoda dan
Siti Mariyam
Erick Wanggai
Hugo Rumbewas
Wanny Kambu
Marlon Iwanggin
Yerri Mundoni
Lidia Kandipi
Jefri Arizona
M. Fajar Marsaoly
Yulius Tata
11
Bivalvia (Moluska) pada ekosistem
Lamun di Kampung Kapisawar
Distrik Meosmanswar Kabupaten
Raja Ampat
4. Komposisi jenis ikan yang
berasosiasi pada hamparan Lamun
di Pulau Mansinam
Verbelina
Baransano
Closing
Debriefing Practice Field Work for Inventorying Marine Invertebrates and
Microbes of in Raja Ampat Islands.
3 & 4 Genetic Basics, General Course and Training Genetic Basic
Agenda
Time
Activities
Presenter
Thursday, April 11, 2013
09.00 – 09.30
Opening
Head of Fishery
Departmen, FPPK
09.30 – 11.30
General Course: Conservation Genetics
Abdul Hamid Toha
11.30 –12.00
Discussion
Moderator
13.00 – 14.00
Meeting
14.00 – 16.00
Genetic Basics
Friday, April 12, 2013
Fishery Department
Abdul Hamid Toha
09.30 - 10.30
Socialization of PEER project
Abdul Hamid Toha
14.00 – 16.00
Practicum: Extraction, PCR, Electrophoresis
Andry Kuncoro
12
General Course
Training Genetic Basics
13
RESEARCH
Field Work
No
1
2
3
4
Species
n
Ekstraction
PCR
Seq
Note
Acteon tornalitis
3
No
No
No
No
Actinopyga palauensis
1
No
No
No
No
Atilia ocelata
3
No
No
No
No
Bohadsia marmorata
4
No
No
No
No
5
Bolmaguttata millegranosa
1
No
No
No
No
6
Casidula vespertillionis
4
No
No
No
No
7
Chicoreus capucinus
3
No
No
No
No
No
No
No
No
9
Euapta godeffroyi
Fissilabia decollate
5
3
No
No
No
No
10
Herpetopoma atrata
3
No
No
No
No
11
Hippopus hippopus
3
No
No
No
No
12
1
No
No
No
No
13
Holothuria fuscopuntata
Littorina cocinea
5
No
No
No
No
14
Littorina scabra
3
No
No
No
No
15
Littorina sp
3
No
No
No
No
No
No
No
No
17
Nerita costata
Nerita undata
4
3
No
No
No
No
18
Nerita planespira
3
No
No
No
No
19
Opheodesoma sp
5
No
No
No
No
20
Panulirus versicolor
Yes
Yes
Yes, 32
No
21
Polymesoda spp
42
4
No
No
No
No
22
Sipunculus nudus
5
No
No
No
No
Stichopus variegates
1
No
No
No
No
8
16
23
24
Telebraria palustris
7
No
No
No
No
25
Telescopion-telescopion
1
No
No
No
No
26
Terebrallia sulcata
3
No
No
No
No
27
Tripneutes gratilla
Yes
Yes
Yes, 11
No
28
Tridacna crocea
11
3
No
No
No
No
29
Tridacna squamossa
3
No
No
No
No
30
Tridacna maxima
3
No
No
No
No
31
Tridacna gigas
3
No
No
No
No
32
Turritella simplex
1
No
No
No
No
33
Vitoida auqatilis
4
No
No
No
No
151
14
Euapta godeffroyi
E. godeffroyi distributed in Raja Ampat coastal. Habitat of E. godeffroyi is
Intertidal pool, in sand, beneath stones. Ecology: benthic, continental shelf, detritus
feeder, deposit feeder. This species inhabit in depth range 0-77 m. E. godeffroyi or
paraer in local name at Raja Ampat was found in rocky or sandy mud in Waigeo
Island, Raja Ampat
E. godeffroyi. Kingdom Animalia. Phylum Echinodermata. Class Holothuroidea. Order
Apodida. Family Synaptidae. Genus Euapta. E. godeffroyi from Waigeo Island
Specimens in UNIPA Genetic Lab
No ID Sampel
Species
1
UNP.000
E. godeffoyi
Sites
P. Batanta Raja Ampat
n
5
Ext
No
PCR
No
Seq
No
ID Sample
No
1
2
3
4
5
ID Sampel
UNP.000.01
UNP.000.02
UNP.000.03
UNP.000.02
UNP.000.03
Collected Date
08-feb-13
08-feb-13
08-feb-13
08-feb-13
08-feb-13
Locality
P. Batanta
P. Batanta
P. Batanta
P. Batanta
P. Batanta
Exist in
LGU
LGU
LGU
LGU
LGU
Containers
Box R4
Box R4
Box R4
Box R4
Box R4
Fissilabia decollata
F. decollata distributed in Indonesia, including in Raja Ampat. These neatly
spiraled snails sometimes seen, usually in large numbers on rocky shores and seawalls
on our Southern shores. Elsewhere, they are called cluster winks for their habit of
gathering in clusters. But disperse to feed at high tide. The size was about 2cm. The
snails brood their young. Fertilization is internal and fertilized embryos are stored in a
brood pouch behind the head of the female. They are then released into the sea as
free-swimming larvae.
F. decollata found between rocks of Mangroves in Waigeo Island, Raja Ampat. F.
decollata. Kingdom Animalia. Phylum Mollusca. Class Gastropoda. Order
Neogastropoda. Family Planaxidae. Genus Fissilabia.
15
Specimens in UNIPA Genetic Lab
No ID Sampel
Species
1
UNP.000
F. decollata
Sites
P. Batanta Raja Ampat
n
3
Ext
No
PCR
No
Seq
No
ID Sample
No
1
2
3
ID Sampel
UNP.000.01
UNP.000.02
UNP.000.03
Collected Date
08-feb-13
08-feb-13
08-feb-13
Locality
P. Batanta
P. Batanta
P. Batanta
Exist in
LGU
LGU
LGU
Containers
Box R4
Box R4
Box R4
Herpetopoma atrata
H. atrata were distributed in Indonesia including in Raja Ampat water. It
preferred intertidal rocky reefs as its habitat and have size 15–20mm.
H. atrata found in sandy mud (loam) and Mangrove, Batanta Island, Raja Ampat. H.
atrata. Kingdom Animalia. Phylum Mollusca. Class Gastropoda. Order
Archaegastropoda. Family Trochidae. Genus Herpetopoma.
Specimens in UNIPA Genetic Lab
No ID Sampel
Spesies
1
UNP.0040 H. atrata
Lokasi
Raja Ampat
n
3
Eks
No
PCR
No
Seq
No
ID Sample
No
1
2
3
ID Sampel
UNP.0040.01
UNP.0040.02
UNP.0040.03
Collected Date
08-feb-13
08-feb-13
08-feb-13
Locality
P. Batanta
P. Batanta
P. Batanta
Exist in
LGU
LGU
LGU
Containers
Box R4
Box R4
Box R4
Holothuria fuscopunctata
H. fuscopunctata was also found in Raja Ampat. This species is deep
creases in the thick body wall and distinct fine spots easily distinguish this large
holothurian from others. H. fuscopunctata is more common in water deeper than about
10 metres on sand or a mixture or rubble and sand. It occurs at the outer barrier reefs
as well as other mid-shelf reefs.
H. fuscopunctata foun in rocky/sandy mud, Waigeo Island, Raja Ampat. H.
fuscopunctata. Kingdom Animalia. Phylum Echinodermata. Class Holothuroidea. Order
Aspidochirotida. Family Holothuriidae. Genus Holothuria.
16
Specimens in UNIPA Genetic Lab
No ID Sampel
Spesies
1
UNP.0039 H. fuscopuntata
Lokasi
P. Batanta Raja Ampat
n
3
Eks
No
PCR
No
Seq
No
ID Sample
No
1
2
3
ID Sampel
UNP.0039.01
UNP.0039.02
UNP.0039.03
Collected Date
08-feb-13
08-feb-13
08-feb-13
Locality
P. Batanta
P. Batanta
P. Batanta
Exist in
LGU
LGU
LGU
Containers
Box R4
Box R4
Box R4
Hippopus hippopus
Hippopus is a genus of large tropical saltwater clams, marine bivalve mollusks in
the family Tridacnidae, the giant clam family. This species distributed in Raja Ampat
coastal. The shell of this clam is very thick with prominent ribs, and has reddish
blotches making it easy to differentiate from other clams. The mantle of these clams
does not extend past the edge of the shell, and are brownish-green to gray in color with
faint gold stripes. The large opening in the mantle, which is called the incurrent
aperture, lacks the tentacles that are common with other clams within
the Tridacnidae family.
Kima H. hippopus in rocky coral Batanta Island, Raja Ampat. H. hippopus. Kingdom
Animalia. Phylum Mollusca. Class Bivalvia. Order Veneroida. Family Tridacnidae.
Genus Hippopus.
Specimens in UNIPA Genetic Lab
No ID Sampel
Spesies
1
UNP.0019 H. hippopus
Lokasi
P. Batanta Raja Ampat
n
3
Eks
No
PCR
No
Seq
No
ID Sample
No
1
2
3
ID Sampel
UNP.0019.01
UNP.0019.02
UNP.0019.03
Collected Date
08-feb-13
08-feb-13
08-feb-13
Locality
P. Batanta
P. Batanta
P. Batanta
Exist in
LGU
LGU
LGU
Containers
Box R4
Box R4
Box R4
Tridacna sp
Tridacna sp. features a deeply folded, almost zig-zag dorsal shell margin,
reminiscent of the distantly related T. gigas. This character allows for an easy
distinction from the two Red Sea congeners, the fluted clam (T. squamosa Lamarck
1819) and Tridacna maxima Ro¨ding 1798. The latter two species show only weakly
sinusoidal dorsal shell margins. The species occurs throughout the Indonesia area
including in Raja Ampat. Live specimens were found exclusively in very shallow water
17
including reef flat, sea grass beds, sandy-rubble flats, on slight depressions in barren
rocky flat, or under branching corals orcoral heads, shallower than 2 m.
(a) T. costata
(b) T. maxima
(c) T. squamosa
Tridacna sp in rocky coral in Batanta Island, Raja Ampat. T. costata. Kingdom
Animalia. Phylum Mollusca. Class Bivalvia. Order Veneroida. Family Tridacnidae.
Genus Tridacna.
Specimens in UNIPA Genetic Lab
No
1
No
1
No
1
No
1
ID Sampel
UNP.0021
ID Sampel
UNP.0017
ID Sampel
UNP.0018
ID Sampel
UNP.0020
Species
T. crocea
Species
T. squamosa
Species
T. maxima
Spesies
T. gigas
ID Sample
No ID Sampel
1
UNP.0021.01
2
UNP.0021.02
3
UNP.0021.03
No ID Sampel
1
UNP.0017.01
2
UNP.0017.02
3
UNP.0017.03
No ID Sampel
1
UNP.0018.01
2
UNP.0018.02
3
UNP.0018.03
No ID Sampel
1
UNP.0020.01
2
UNP.0020.02
3
UNP.0020.03
Sites
P. Batanta Raja Ampat
Sites
P. Batanta Raja Ampat
Sites
P. Batanta Raja Ampat
Sites
P. Batanta Raja Ampat
Collected Date
08-feb-13
08-feb-13
08-feb-13
Collected Date
08-feb-13
08-feb-13
08-feb-13
Collected Date
08-feb-13
08-feb-13
08-feb-13
Collected Date
08-feb-13
08-feb-13
08-feb-13
Locality
P. Batanta
P. Batanta
P. Batanta
Locality
P. Batanta
P. Batanta
P. Batanta
Locality
P. Batanta
P. Batanta
P. Batanta
Locality
P. Batanta
P. Batanta
P. Batanta
n
3
n
3
n
3
n
3
Ext
No
Ext
No
Ext
No
Ext
No
Exist in
LGU
LGU
LGU
Exist in
LGU
LGU
LGU
Exist in
LGU
LGU
LGU
Exist in
LGU
LGU
LGU
PCR
No
PCR
No
PCR
No
PCR
No
Seq
No
Seq
No
Seq
No
Seq
No
Containers
Box R4
Box R4
Box R4
Containers
Box R4
Box R4
Box R4
Containers
Box R4
Box R4
Box R4
Containers
Box R4
Box R4
Box R4
Panulirus versicolor
P. versicolor (Latreille, 1804) is one of the six varieties of spiny lobster that
found in the Indonesian Archipelago including in Raja Ampat waters. The other five
being P. homarus, P. longipes, P. penicillatus, P. polyphagus and P. ornatus (Girsang
et al 1995). P. versicolor grows up to 40 centimetres. Like all spiny lobster, this animal
18
has no claws, two spiny rostra over the eyes, and two pairs of large antennae. Both
antennae are usually white with the first pair are double ended and the second hard and
spiny. The carapace is white, pink and black, with horizontal bands and a reticulate.
P. versicolor. Kingdom animalia, Phylum arthropoda, subphylum Crustacea, Class
Malacostraca, Ordo Decapoda, Family Palinuridae, Genus Panulirus.
Telescopium telecospium
T. telescopium is commonly seen in Indonesia mangroves including in Raja Ampat, on
mud, sometimes in the hundreds covering a large area. This large snail has a tall,
conical shell strongly reminiscent of an ice cream cone. Features of T. telecospium are
8-15cm. The Operculum of Telescopium is small and circular. The animal is velvety
black with a highly extendible proboscis. There is a third eye on its mantle margin, in
addition to a pair of eyes at the tentacles. It can stay out of water for long periods of
time. Telescopium feeds on detritus and algae from the mud surface at low tide by
sucking using its proboscis (Reid et al., 2008).
T. telescopium found in Mangrove and Mud, Batanta Island, Raja Ampat. T.
telescopium. Kingdom Animalia. Phylum Mollusca. Class Gastropoda. Order
Sorbeoconcha. Family Potamididae. Genus Telescopium.
Tripneutes gratilla
T. gratilla, a sea urchin widely distribution throughout the Indo-Pacific including
in Raja Ampat. This species is ecologically important, especially in sea grass habitats
and is a food source with good potential for aquaculture. Habitat of T. gratilla are
sheltered and moderatley exposed reef. They can reach 10 to 15 centimetres (4 to 6 in)
in size. They are dark in colour, usually bluish-purple with white spines. The pedicles
are also white, with a dark or black base. The spines of some specimens are wholly
orange, while those of others are only orange-tipped or completely white. This colour
disappears when the individual dies or is taken out of the ocean, and is difficult to
preserve.
19
T. gratilla. Kingdom Animalia. Phylum Echinodermata. Class Echinodea. Order
Temnopleuroida. Family Toxopneustidae. Genus Tripneutes.
Vitoida auqatilis
V. auqatilis, found on Mangrove leaves and muddy loam in Batanta Island, Raja
Ampat. V. auqatilis. Genus Vitoida.
Littorina sp
Height commonly exceeding 5 mm, and in some species slightly exceeding 1.5
cm; operculum horny ; periostracum not uniformly tan or brown (Almagorda
subrotundata is brown, but usually has spiral banding ) (mostly at higher tide levels on
rocly shores, or on rocks, concrete and wood in bays , sometimes in salt marshes). The
periwinkles are found on the seashore in the littoral zone and sub littoral zone in all
parts of the Raja Ampat.
Littoraria scabra, common name: the mangrove periwinkle, is a species of sea
snail, a marine gastropod mollusk in the family Littorinidae, the winkles or periwinkles.
The size of an adult shell varies between 15 mm and 40 mm. This species is distributed
in Indonesia including in Raja Ampat. Littoraria coccinea is a species of sea snail, a
marine gastropod mollusk in the family Littorinidae, the winkles or periwinkles.
Littorina sp, found on Mangrove leaves and muddy loam in Batanta Island, Raja Ampat
20
Lab Work
PCR Products
21
Sequencing Results
Data Analyses
22
Presentation
23
PUBLICATIONS
1. Website: http://www.ibcraja4.org/
24
2. Monthly Newsletters: http://ibcraja4.org/page/read/newsletters
June 2012
September
2012
December 2012
July 2012
August 2012
October 2012
November 2012
January 2012
Pebruary 2013
25
March 2012
April 2013
3. Article 1: http://berkalahayati.org/index.php/bph/article/view/642
26
Article 2: http://berkalahayati.org/index.php/bph/article/view/658
27
4. Cover of Book Manuscript (in Press)
Abdul Hamid A. Toha
Sutiman B. Sumitro
Luchman Hakim
Widodo
KEANEKARAGAMAN DAN
KONSERVASI BULU BABI
28
Information on the numbers and types of students and other junior collaborators involved in the
project.
Name
(Gender)
Undergraduate (Field Trip)
No
1
Jefri Arizona
(Male)
2
David Lomban
(M)
3
Rico Ricardo
Mailisa (M)
4
Hugo
Rumbewas (M)
Title
Asosiasi dan Komposisi Ikan pada
Ekosistem Lamun di Distrik Amdui
Kabupaten Raja Ampat
Komposisi Jenis Gastropoda pada
Ekosistem Mangrove di Kampung
Sawinggrai Distrik Meosmanswar
Kabupaten Raja Ampat
Sebaran dan Pemanfaatan Filum
Sipuncula di Kampung Amdui Distrik Selat
Sagawin Kabupaten Raja Ampat
Budidaya kerang mutiara (Pinctada sp) di
Kampung Amdui Distrik Selat Sagawin
Kabupaten Raja Ampat
Inventarisasi Makrozoobenthos di
Ekosistem Mangrove pada Kampung
5
Amdui Distrik Selat Sagawin Kabupaten
Raja Ampat
Asosiasi gastropoda pada ekosistem
Deasy Yamco
6
Mangrove di Kampung Amdui Selat
(F)
Sagawin Kabupaten Raja Ampat
Kelimpahan Jenis Kima (Tridacnidae) di
Absalom
7
Kampung Amdui Selat Sagawin Kabupaten
Wakum (M)
Raja Ampat
Kelimpahan Jenis Teripang di Kampung
Ronald
8
Sawinggrai Distrik Meosmanswar
Hambuako (M)
Kabupaten Raja Ampat
Kelimpahan jenis Bia Kodok (Polimesoda
Steven Ayomi
9
spp) di Kampung Amdui Distrik Selat
(M)
Sagawin Kabupaten Raja Ampat
Kelimpahan jenis Gastropoda dan Bivalvia
(Moluska) pada ekosistem lamun di
10 Yulius Tata (M)
Kampung Kapisawar Distrik Meosmanswar
Kabupaten Raja Ampat
Other junior collaborators-Capacity Building in Research
Putri P.
Ngilawane
(Female)
Department
(Faculties), Goal
Fishery (Faculty of
Husbandry, Fishery
and Marine SciencesFPPK) Field Work
Fishery (FPPK), Field
Work
Fishery (FPPK), Field
Work
Fishery (FPPK), Field
Work
Fishery (FPPK), Field
Work
Fishery (FPPK), Field
Work
Fishery (FPPK), Field
Work
Fishery (FPPK), Field
Work
Fishery (FPPK), Field
Work
Fishery (FPPK), Field
Work
1
Nurhani
Widiastuti,S.Pi,
M.Si (F)
Partisipasi masyarakat local dalam
pengelolaan ekowisata di Kampung X Raja
Ampat
Fishery (FPPK,
UNIPA), Capacity
building in research
2
Fanny
Simatauw, S.Pi,
M.Si (F)
Partisipasi masyarakat local dalam
pengelolaan ekowisata di Kampung X Raja
Ampat
Fishery (FPPK,
UNIPA), Capacity
building in research
29
3
Juliana
Leiwakabessy,
S.Pi, M.Si (F)
Ekologi Molekuler Ketam Kenari (Birgus
Latro L.) di Raja Ampat dan Manokwari
Fishery (FPPK,
UNIPA), Capacity
building in research
4
Ida Lapadi,
S.Pi, M.Si (F)
Ekologi Molekuler Ketam Kenari (Birgus
Latro L.) di Raja Ampat dan Manokwari
Fishery (FPPK,
UNIPA), Capacity
building in research
5
Tresia
Tururaja,. S.Ik,
M.Si (F)
Identifikasi Kima (Tridacna) Dengan
Menggunakan Pendekatan Metode DNA
Barcoding dan Variasi Genetik di Perairan
Raja Ampat Papua Barat
Seleksi dan domestikasi kandidat indukan
udang galah Macrobachium rosenbergii (de
Robi Binur,
6
Man, 1879) asal Papua berdasarkan marka
S.Si, M.Si (M)
genetik untuk pengembangan indukan
baru.
Undergraduate (Research)
Fishery (FPPK,
UNIPA), Capacity
building in research
Biology (Faculty of
Sciences, UNIPA),
Capacity building in
research
Identifikasi spesies laut menggunakan
pendekatan DNA barcoding
Marine Sciences,
Thesis
2
Nur Aisyah
Hasniar (F)
Variasi Warna Menunjukkan Keragaman
Genetik Teripang (Holothuridea) Di
Perairan Raja Ampat
Biology (Faculty of
Sciences), Thesis
3
Bayu Pranata
(M)
Bioekologi dan filogeni ketam kenari Raja
Ampat
Fishery (FPPK),
Thesis
4
Masriana (F)
Identifikasi kima (Tridacna) dengan
menggunakan DNA barcoding dan variasi
genetik di Perairan Raja Ampat, Teluk
Doreri dan Teluk Wondama
Marine Sciences,
Thesis
5
Herimiel
Ambrauw (M)
Kajian kearifan local dalam bentuk
penerapan sasi hasil laut di Distrik Kofiau,
Kabupaten Raja Ampat
Fishery Sciences,
Thesis
Distribusi spasial dan keragaman genetik
bulu babi (Tripneustes gratilla) di Pulau
Ambon dan Raja Ampat
Biology (Faculty of
Sciences, Brawijaya
University), Thesis
Eksistensi sosial-lingkungan dalam
ekowisata di Kepulauan Raja Ampat
Social Economy
(Agriculture Faculty of
UNIPA-Brawijaya
University),
Dissertation
1
Kuncoro (M)
Master Students
6
Veince Silahooy
(M)
Doctoral Students
7
Jumiati Mustiah
(F)
30
Laboratory Technicians, Newsletter contributors, Website operators
1
Jehan Haryati, S.Si, M.Si (F)
Admin staff
Biology Graduated student, UB
2
Q. Mohammed (M)
IT staff
Biology Graduated student, UB
3
M. Dailami, S.Si (M)
Admin/technician
Chemistry Graduated (Faculty of
Sciences, UNIPA), Capacity
building in research
4
Andyka AriWibowo, S.Ik (M)
Technician
Marine Sciences Graduated,
Thesis
5
Nurhani Widiastuti, S.Pi, M.Si
(F)
Admin staff
Faculty of UNIPA
6
Prof. Sutiman B. Sumitro,
D.Sc
Consultant,
Trainer
Faculty of Brawijaya University
7
M. Takdir
Newsletter
contributor
Faculty of UNIPA
8
Widodo, Ph.D
9
L. Hakim, Ph.D
10
Jeni, S.Pd, M.Si
Researcher
Faculty of UNIPA
11
Hamid Toha
PI, Trainer
Faculty of UNIPA
12
Robi Binur
13
Yuliana L, S.Pi, M.Si
14
Irma A., S.Ik, M.Si
15
Hermawaty A., S.Si, M.Si
16
Lutfi
17
Kuncoro
Researcher,
Coauthor
Researcher,
Trainer
Contributor in
newsletter
Contributor in
newsletter
Contributor in
newsletter
Contributor in
newsletter
Contributor in
newsletter
Technician
Faculty of Brawijaya University
Faculty of Brawijaya University
Faculty of UNIPA
Faculty of UNIPA
LIPI staff
Faculty of UNIPA
Faculty of UNIPA
UNIPA student