IntelliQube

IntelliQube®
Fully automated PCR setup,
amplification and analysis system
The IntelliQube is the first fully automated and highthroughput instrument featuring seamless integration
of liquid handling, thermal cycling, detection, and data
analysis. The IntelliQube supports quantitative real-time
PCR, end-point PCR, and isothermal chemistries, allowing
you to realize the benefits of inline automation enabled by
the innovative Array Tape® consumable.
Integrated
The IntelliQube integrates the PCR process by combining multiple instruments into one, simplifying your workflow for walkaway operation. Simply load and go, freeing up lab space and allowing personnel to focus less on managing instruments and
more on their research.
Efficient
The IntelliQube and Array Tape consumable offer a scalable, high-throughput solution for your laboratory.
With our new 768-well format, you will realize twice the PCR throughput
compared to traditional 384-well qPCR instruments. For end-point genotyping,
the IntelliQube combined with the Hydrocycler™ allows processing of up to 50
microplate-equivalents or 19,000 reactions per day!
Economical
Substantially reduce your assay volumes with miniaturized reaction wells on the
innovative Array Tape consumable. You can save up to a 90% in your assay costs
with 1.6μL reaction volumes!
Flexible
The IntelliQube is compatible with a broad range of chemistries and fluorogenic
probes. Integrated liquid handling maximizes your run flexibility and minimizes
waste compared to rigidly defined chips, microarrays or microfluidics. A variety
of qPCR and end-point PCR applications are compatible with the system,
including but not limited to:
• Gene Expression Analysis
• Genotyping and Copy Number Variants (CNVs)
• Microbial Detection and Quantification
• miRNA Analysis
• Zygosity and GMO Detection
Your Lab Will Realize
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Walk-away operation
Increased efficiency of lab personnel
Reduced chemistry cost
Increased throughput
Reduced consumable costs
High-Performance
Industry-leading liquid handling, thermal cycling and optical systems come together in the IntelliQube to deliver the accurate
and sensitive PCR results you expect, with the flexibility, efficiency, and integration that your lab needs.
Exclusive distribution of the
Douglas Scientific® Array Tape® Platform
in Europe, Middle East and Africa.
3/2015
Liquid Handling
IntelliQube leverages best-in-class multi-functional dispensing for high speed liquid
handling in 384- or 768-well Array Tape. Precise sample dispensing into Array Tape is
handled by an interchangeable CyBi® FeliX 96- or 384-channel pipette head. Reagent
dispensing is performed by the Douglas Scientific Dispense Jet, a 4-channel, non-contact
dispense head that loads the assay mixtures required for your protocols. With CVs less than
5%, the liquid handling on the IntelliQube offers exceptional repeatability and data quality.
Pipette Wash
Dispense Jet
The IntelliQube Pipette Wash system reduces tip waste and associated consumable costs
while mitigating the risk of cross contamination. The Pipette Wash uses fluid agitation
around the dispense tips to ensure complete interior and exterior cleaning of the tips,
while a touch-off feature is used to counteract surface tension and remove residual
droplets. Complete elimination of the wash basin contents after each wash cycle
combined with the ability to incorporate a wash additive, such as bleach, ensures testing
integrity and minimizes the risk of cross-contamination.
Pipette and Jet Washes
Liquid Handling Specifications
SAMPLE DISPENSING
CYBI® FELIX PIPETTE HEAD
ASSAY DISPENSING
DISPENSE JET
TOTAL DISPENSING TIME
3/2015
Mechanism
Air displacement
Dispensing Configuration
96- or 384-channel
Dispense Volume (at launch)
800 nL
Precision
≤5%
Recommended Input
Nucleic acid templates
Source Plate Positions
10 (ambient temperature)
Supported Source Plates
ANSI/SBS compliant 96/384-well formats ≤15 mm in height
Mechanism
Single jet solenoid valve
Dispensing Configuration
4-channel
Dispense Volume (at launch)
800 nL
Precision
≤5%
Recommended Input
2X Primer+Probe+Mastermix
Source Plate Positions
3 (temperature controlled)
Supported Source Plates
ANSI/SBS compliant 96-well formats ≤ 30 mm in height
Approximately 5 to 6 minutes per array
Thermal Cycling
Thermal Cycling Specifications
The IntelliQube supports inline
amplification with a Peltier thermal
cycling block designed specifically for
Array Tape. Due to the unique block
design and surface geometry, both 384and 768-well Array Tape are supported
without requiring a block change.
Excellent ramp rates, temperature
uniformity and accuracy enable broad
compatibility with amplification
methodologies, including fast PCR and
isothermal chemistries such as DNAble®.
BLOCK TYPE
Peltier
BLOCK CONFIGURATION
384/768-well Array Tape
TEMPERATURE RANGE
20 - 100°C
TEMPERATURE ACCURACY
±0.25°C
TEMPERATURE UNIFORMITY
±0.5°C at 60°C
HEATING RAMP RATE
3.0°C/sec
COOLING RATE
3.0°C/sec
Instrument Interior
*DNAble is a registered trademark of EnviroLogix
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Hydrocycler™
Combining throughput, efficiency and robust design, the Hydrocycler offers a high throughput alternative to inline
amplification for end-point PCR with Array Tape. With three precise temperature-controlled waterbaths, the system is optimized
to rapidly thermal cycle up to 50 arrays per run. End-point fluorescence can be read with the IntelliQube following PCR, enabling a
5-fold increase in end-point throughput when compared with inline thermal cycling.
Detection
The IntelliQube features an advanced optical system for inline fluorescence detection in Array Tape. Filtered LEDs are optimized for
a variety of commonly used fluorophores with the excitation range of 480-620 nm. A high-resolution CCD camera supports
detection of fluorescence intensity within five detection channels for dyes with the emission range of 510-705 nm. Multiplex data
capture for all five optical channels can occur in as little as 10 seconds.
FAM™, JOE™, VIC, HEX™, NED™, TAMRA™ and ROX™ are trademarks of Life Technologies Corporation and its subsidiaries. VIC® and Texas Red® are registered
trademarks of Life Technologies Corporation and its subsidiaries. Cy5® is a registered trademark of GE Healthcare Bio-Sciences Corp. Cal Fluor® and Quasar®
are registered trademarks of Biosearch Technologies, Inc.
Detection Specifications
3/2015
EXCITATION SOURCE
15 Filtered LEDs
EXCITATION RANGE
480-620 nm
DETECTION METHOD
CCD
DETECTION RANGE
510-705 nm
MULTIPLEX DATA CAPTURE TIME
≤10 seconds
Thermal Cycling and Detection
Software and Data Analysis
Intellics® is an innovative software product that provides centralized data management, instrument monitoring, run optimization,
protocol generation and streamlined data analysis for the IntelliQube. Intellics is pre-loaded on each instrument and is accessible
to users through a unified web-based interface that allows easy multi-user access.
Intellics features two additional applications, Array Builder™ which allows users to rapidly generate liquid handling and thermal
cycling protocols, and IntelliScore® for post-PCR data analysis and interpretation. A variety of PCR experiment types are supported
by IntelliScore, including absolute quantification (standard curve), relative quantification (relative standard curve and ΔΔCq),
genotyping and presence/absence.
A touch screen interface facilitates instrument control and also supports real-time data monitoring.
Instrument Control and Software Specifications
SOFTWARE
Intellics Software Suite with IntelliScore
OPERATING SYSTEM
Microsoft Windows® Embedded Standard 7
INSTRUMENT CONTROL INTERFACE
Full HD touch screen display
ANALYSIS AND PROTOCOL SET UP
Network access using Google Chrome™
SUPPORTED ANALYSIS MODES
Standard Curve, Relative Curve, ΔΔCq, Genotyping, Presence/Absense
DATA EXPORT AND LIMS INTEGRATION
Configurable comma separated values (CSV) files
COMMUNICATION
RJ-45 Gigabit LAN
Figure 1: Two-Fold Resolution with
5,000 and 10,000 copies gDNA
Figure 2: Excellent Cq uniformity across an
entire array with 10,000 copies gDNA
Figure 3: Wide dynamic range with a
10-fold plasmid DNA serial dilution
*For more information see the IntelliQube Characterization Application Note at DouglasScientific.com
Additional Performance Specifications
DYNAMIC RANGE
8 Log
RESOLUTION
2 Fold
CQ UNIFORMITY
+ 0.5 Cq
ESTIMATED QPCR RUN TIME
Standard: 75 minutes per array
Fast: 35 minutes per array
ESTIMATED THROUGHPUT
PER 8 HOURS
Six 768-well arrays
4,600 data points
The products of Douglas Scientific LLC are not FDAapproved for use in clinical diagnostic procedures.
Certain applications of this product are covered by
patents issued to parties other than Douglas Scientific
and applicable in certain countries. Purchase of this
product does not include a license to perform any such
applications. Users of this product may therefore be
required to obtain a patent license depending upon the
particular application and country in which the product
is used. In particular, use of this product in conjunction
with an intercalating dye may be covered by one or more
of the following U.S. patents and corresponding claims
outside the U.S.: U.S. 5,994,056 and U.S. 6,171,785. Further
information on purchasing licenses can be obtained from
Roche Molecular Systems, Inc., 1080 U.S. Highway 202
Branchburg, New Jersey 08876.
Patents pending
Exclusive distributor of the Douglas Scientific®
Array Tape® Platform in Europe, Middle East and Africa.
3/2015
Unit 1-2 Trident Industrial Estate • Pindar Road
Hoddesdon • Herts • EN11 OWZ • UK
Tel: +44 (0)1992 470 757
Fax:+44 (0)1438 900 670
Email: [email protected]
www.lgcgroup.com/douglas-scientific
Manufactured by:
®