Puredown Protein A/G-Agarose Conjugate
Transcription
Puredown Protein A/G-Agarose Conjugate
Puredown Immunoprecipitation of your proteins Puredown Protein A/G-Agarose Conjugate Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure. Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. Puredown Protein A/G-Agarose are covalently coupled to highly cross-linked agarose bead. This product is provided preblocked with fatty acid free bovine serum albumin and have been extensively washed to reduce non-specific binding. Formulations are ready to use and 10ul of agarose per 1ug of primary antibody can be used in standard immunoprecipitation procedures. Puredown Protein A-Agarose, BSA-binding site blocked Puredown Protein G-Agarose, BSA-binding site blocked Puredown Protein A/G-Agarose, BSA-binding site blocked Bac uced kgro und Reduced Background Minus albumin-binding region to reduce non-specefic binding Convenient Ready to us for immunoprecipitation procedures Economy Cost less than other competitor's Protein A/G-Agarose WWW.GenDEPOT.COM Ordering Information Product Red Cat No Size P9201-015 P9201-050 P9201-100 P9201-200 P9202-015 P9202-050 P9202-100 P9202-200 P9203-015 P9203-050 P9203-100 P9203-200 1.5ml 5ml 10ml 20ml 1.5ml 5ml 10ml 20ml 1.5ml 5ml 10ml 20ml TO ORDER Toll Free 866.417.0078 Fax 281.579.6876 [email protected] PO Box 454 Barker, TX 77413 U.S Start Small for Big Change