Appendices

Transcription

Appendices

21st Century COE Program
Marine Bio-Manipulation Frontier for Food Production
- Toward Advanced and Safe Use of Aquatic Organisms
Appendices
Steering Committee of the COE Program
Faculty of Fisheries Sciences
Hokkaido University
3-1-1 Minato-cho, Hakodate
Hokkaido 041-08611
JAPAN
Table of Contents
Appendix 1 Members
page 2
Appendix 2 Budgets
page 13
Appendix 3 Summary of Research Results
page 17
Appendix 4 Publication Lists
page 56
Appendix 5 International Symposia
page 94
Appendix 6 International Cooperation
page 109
Appendix 7 Educational Achievements
page 124
Appendix 8 Achievements in Young Scientist Fostering
page 135
1
Appendix 1
Members
The COE program has total membership of 21 researchers, ca 13 post docs, ca 15 research
assistants (doctor course graduate students), ca 2 technical assistants and 3 administration
assistants.
Researchers
page 3
Post-docs
page 7
Research assistants
page 9
Technical assistants
page 11
Administration assistants
page 12
2
Researchers
Kohei YAMAUCHI: Leader, Professor, Faculty of Fisheries Sciences, Hokkaido University
Katsutoshi ARAI: Sub-leader, Professor, Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Bio-manipulation to produce sterile fish as a host for surrogate xenogenesis via
germ-line chimerism.
Cytological and Molecular mechanisms of unreduced gametogenesis in aquatic animals.
Naotsune SAGA: Head of Marine Bio-manipulation Project, Professor, Faculty of Fisheries
Sciences, Hokkaido University
Research subjects
Reproductive-cell Production in vitro
Etsuro YAMAHA: Professor, Field Science Center for Northern Biosphere, Hokkaido
University
Research subjects
Establishment of surrogate-aquaculture-techniques
Yoshiyuki TAKAHASHI: Graduate School of Veterinary Medicine, Hokkaido University
Research subjects
Cryopreservation, in vitro production and manipulation of germ cell in mammals and
fishes
Shinji ADACHI, Professor: Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Molecular regulatory mechanisms of germ cell differentiation and ovarian development
in fish
Yasuaki TAKAGI: Professor, Faculty of Fisheries Sciences, Hokkaido University
3
Research subjects
Hard tissue physiology and application in aquatic animals
Basic and applied physiology of aquatic invertebrates
Taizo MOTOMURA: Professor, Field Science Center for Northern Biosphere, Hokkaido
University
Research subjects
Cell biological analysis on the regulation of algal life cycles.
Chikako NAGASATO: Associate professor, Field Science Center for Northern Biosphere,
Hokkaido University
Research subjects
Cell Biological Studies on the control of life cycle in algae
Takashi TODO: Associate professor, Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Molecular control mechanism of gametogenesis in fish
Production of fish gametes in vitro
Kazuhiro URA: Assistant professor, Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Control of gonadal development in marine invertebrates
Takao OJIMA: Professor, Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Analysis of cell wall structures of marine algae and mass production of algal seedlings
by using polysaccharide-degrading enzymes from marine invertebrates
Hiroyuki MIZUTA: Associate professor, Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Development of seaweed seedling production using their particular reproduction
4
Mamoru YOSHIMIZU: Head of Food Security Project, Professor, Faculty of Fisheries
Research subjects
Health control of aquatic animals and creating a worry-free fisheries product
Akihiko HARA: Professor, Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Biomarker for environmental estrogen
Sanae ARIGA: Professor, Faculty of Agriculture, Hokkaido University
Research subjects
Molecular mechanisms of proliferation, differentiation, apoptosis and transformation in
higher animal cells
Hiroyoshi FUJITA: Professor, Graduate School of Medicine, Hokkaido University
Research subjects
The regulation of hematopoiesis in response to environmental stress
Syuiti ABE: Professor, Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Aquagenomics and Molecular Population Genetics for Management and Sustainable Use
of Marine Animals as Food Resources
Kazuo MIYASHITA: Professor, Faculty of Fisheries Sciences, Hokkaido University
Research subjects
Anti-obesity and anti-diabetic effects of seaweed carotenoid, fucoxanthin.
Anti-inflammatory effect of marine carotenoids.
Development of the platform technologies required to produce nutraceuticals from
marine prodcuts.
Build novel medicinal foods with specific health benefits from seaweeds and fish.
5
Kei-ichi SHIMAZAKI: Professor, Faculty of Agriculture, Hokkaido University
Research subjects
Structure and function of biologically acitve proteins in marine elements
Atsuo KIMURA: Professor, Faculty of Agriculture, Hokkaido University
Research subjects
Evaluation of function of carbohydrate and molecular analysis of glycosidase produced
by aquatics
6
COE Researchers (post-docs)
FY 2004
Dr. Noriko AZUMA
Dr. Akira INOUE
Dr. Noriko URUSHIBARA
Dr. Yukinori KAZETO
Dr. Masao KISHI
Dr. Rie KAZETO
Dr. Taiju SAITO
Dr. Dong-Hwang SHIN
Dr. Kagayaki MORISHIMA
Dr. Hong Mei LI
FY 2005
Dr. Noriko AZUMA
Dr. Hisae KASAI
Dr. Yukinori KAZETO
Dr. Masao KISHI
Dr. Won-sub KIM
Dr. Rie KAZETO
Dr. Taiju SAITO
Dr. Munetaka SHIMIZU
Dr. Kengo SHIMOMURA
Dr. Dong-Hwang SHIN
Dr. Hiroyuki NAKAI
Dr. Toshiaki FUJITA
Dr. Takafumi FUJIMOTO
Dr. Hong Mei LI
7
FY 2006
Dr. Noriko AZUMA
Dr. Haruna AMANO
Dr. Yukinori KAZETO
Dr. Won-sub KIM
Dr. Onam KWON
Dr. Rie KAZETO
Dr. Taiju SAITO
Dr. Suzu SAKAO
Dr. Munetaka SHIMIZU
Dr. Kengo SHIMOMURA
Dr. Dong-Hwang SHIN
Dr. Hiroyuki NAKAI
Dr. Eun-Jeong Park
Dr. Toshiaki FUJITA
FY 2007
Dr. Noriko AZUMA
Dr. Haruna AMANO
Dr. Yukinori KAZETO
Dr. Won-sub KIM
Dr. Onam KWON
Dr. Rie KAZETO
Dr. Taiju SAITO
Dr. Dong-Hwang SHIN
Dr. Hiroyuki NAKAI
Dr. Eun-Jeong Park
Dr. Toshiaki FUJITA
8
Research assistants (doctor course students)
FY 2004
Ms. Haruna AMANO
Mr. Syuji OTSUKA
Ms. Suzu SAKAO
Ms. Kanae SATO
Mr. Wataru SABURI
Mr. Mikimasa JO
Ms. Yumiko YASUI
Mr. Muenguen YOON
FY 2005
Ms. Haruna AMANO
Mr. Syuji OTSUKA
Ms. Izumi KONISHI
Ms. Suzu SAKAO
Mr. Wataru SABURI
Ms. Miki FUKUDA
Mr. Hayato MAEDA
Ms. Yumiko YASUI
Mr. Muenguen YOON
FY 2006
Mr. Shingo ARATO
Mr. Kei KIMURA
Ms. Hong LEI
Ms. Izumi KONISHI
Mr. Tomoyuki SASAKI
Ms. Tomoko SHIMIZU
9
Mr. Megumu TAKAHASHI
Mr. Sung Je CHOI
Ms. Miki FUKUDA
Mr. Muenguen YOON
Mr. Hiroyuki YOSHIKAWA
Mr. Rahman MD. MORSHEDUR
FY 2007
Mr. Tomoki ABE
Mr. Shingo ARATO
Mr. Kurin IIMURA
Mr. Tomoyuki OKUMURA
Mr. Kei KIMURA
Mr. Yuya KUMAGAI
Ms. Izumi KONISHI
Mr. Tomoyuki SASAKI
Mr. Yu CHAI
Ms. Tomoko SHIMIZU
Mr. Megumu TAKAHASHI
Mr. Sung Je CHOI
Mr. Yukihiro YAMAMOTO
Ms. Jeong Nam YU
Mr. Hiroyuki YOSHIKAWA
10
FY 2004
Ms. Tomoko KAMEYAMA
Ms. Ayako KUDO
Ms. Ikuko SAKAKIDA
Ms. Yukari KOGA
FY 2005
Ms. Tomoko KAMEYAMA
Ms. Ayako KUDO
Ms. Ikuko SAKAKIDA
Ms. Yukari KOGA
FY 2006
Ms. Ayako KUDO
Ms. Chinatsu MAITA
FY 2007
Ms. Ayako KUDO
Ms. Chinatsu MAITA
11
FY 2004-2007
Ms. Ikuko KOBAYASHI
Ms. Megumi HATAKEYAMA
Ms. Nana EBIKO
12
Appendix 2
Budgets
FY 2004
page 14
FY 2005
page 15
FY 2006
page 16
13
Final Statements of the Budget FY 2004 (Yen)
Cost indexes
Equipments
Travel expenses
Total amount
Items
68,814.000 Aquaculture system
Cell sorter system
29,768,000
DNA sequencer
19,845,000
Fluorescence stereo microscope
2,278,000
Others
6,938,000
14,228,000 International travel
3,532,000
Domestic travel
8,717,000
Invitation expenses
1,816,000
Others
Personal costs
Supplies expenses
163,000
34,897,000 COE researchers (post-doc)
6,776,000
2,689,000
2,889,000
Others
1,400,000
76,090,000 Supplies expenses
55,912,000
International symposia
3,049,000
Publications
1,657,000
Others
15,472,000
8,971,000 Ambitious proposal projects
young scientists
Total
21,143,000
and others
Research grants for
9,985,000
203,000,000
14
8,971,000
Cost indexes
Equipments
Total amount
Items
5,762,000 Aquaculture system
Gene transport system
Others
Travel expenses
Personal costs
8,473,000
Invitation expenses
8,276,000
12,692,000
7,278,000
5,661,000
915,000
13,629,000
3,012,000
Oonuma Workshop for Young Scientists
568,000
Out leach program (Open lecture)
881,000
Publications
6,603,000
Others
3,277,000
young scientists
Total
83,944,000
Others
Research grants for
5,906,000
Domestic travel
and others
2,864,000
424,000
Supplies expenses
2,474,000
173,001,000
15
6,124,000
Cost indexes
Equipments
Travel expenses
Personal costs
Total amount
Items
18,107,383 Aquaculture system
Real time PCR system
4,704,000
Stereo microscope system
1,033,095
Spectrophotometer system
1,201,200
Liquid chromatograph system
4,885,650
Others
3,384,598
9,487,733
Domestic travel
6,942,786
Invitation expenses
1,948,822
Research grants for
5,643,002
525,000
9,225,006
261,795
Oonuma Workshop for Young Scientists
408,382
Out leach program (Open lecture)
1,000,000
Publications
2,323,164
Others
7,591,715
young scientists
Total
18,179,069
4,023,574
and others
72,368,660
Others
Supplies expenses
2,898,840
161,880,000
16
3,885,829
Appendix 3
Summary of Research Results
Marine Bio-Manipulation Project
page 18
R01
Mechanism of gametogenesis in teleost fish
page 20
R02
Analyses on cellular and molecular mechanisms for unreduced ametogenesis
page 22
R03
Studies on behavior of the cytoplasmic factors including organelles during
gametogenesis and zygote development
page 24
R04
Establishment of sterilization technology for host individuals in germ-line
chimera formation
page 26
R05
Establishment of several surrogate-aquaculture-techniques
page 28 R06 The formation of a seaweed genome consortium in Asian - Pacific regions
page 30
R07
Research and development for the establishment of research infrastructures
of model marine plants and genome sciences
page 32
R08
Studies on the molecular and cellular biology in the sexual differentiation
and gemetogenesis of the seaweeds
page 34 R09 Clone seeds
page 36 R10 Hard tissue research and application in aquatic animals
page 38
R11
Physiology of marine invertebrates: a molecular approach
Food Security Project
page 40 R12 “Epidemic prevention” to avoid diseases spreading via seeds
page 42
R13
Molecular population genetics of Pacific salmon for their management and
sustainable use
page 44 R14 Genetic variation and population structure of flatfish
page 46 R15 Monitoring estrogenic contaminations in aquatic environments using fish
serum proteins
page 48 R16 Molecular Mechanisms for Safety of Lives
page 50
R17
Multi-functionalities of seaweed carotenoid, fucoxanthin
page 52 R18 Functional marine lipids and its utilization
page 54 R19 “Safety controls” to ensure safety and reliability of seafoods
17
R01
Mechanism of gametogenesis in teleost fish
Marine Bio-Manipulation Project (Mechanism Analysis, germ-cell differentiation, In vitro gamete
production)
Member
Kohei Yamauchi, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Shinji Adachi, Faculty of Fisheries Sciences, Hokkaido University
Takashi Todo, Faculty of Fisheries Sciences, Hokkaido University
Shigeho Ijiri, Faculty of Fisheries Sciences, Hokkaido University
Akihiko Hara, Faculty of Fisheries Sciences, Hokkaido University
Katsutoshi Arai, Faculty of Fisheries Science, Hokkaido University
Etsuro Yamaha, Field Science Center for Northern Biosphere, Hokkaido University
Background, Objectives and Research Outline
In order to control the reproduction of aquatic animals, it is necessary to know the regulatory mechanism of
the gametogenesis, especially oogenesis in detail. We have investigated the molecular mechanism of the
oogenesis using in vitro culture systems of ovarian fragments at various stages through analyses of the
reproduction-related factors, for example, pituitary gonadotropic hormone (GTH), ovarian steroidogenic
enzymes, hormone receptors and so on, in the Japanese eels (Anguilla japonica). The Japanese eels have
immature ovaries and further oogenesis is arrested under captive conditions. However, ovarian
development can be induced by the administration of salmon pituitary extract (SPE) richly including GTH.
Since effects of exogenous hormones can therefore be readily assessed, the Japanese eel would be a
suitable model for the study of reproductive physiology in fish. In this project, we have focused on the
early (previtellogenic) and the final (post-vitellogenic) stages of oogenesis in the Japanese eel.
Previtellogenic oocyte growth in the Japanese eel is mainly characterized by the appearance and
accumulation of oil droplets in the oocyte. However, the molecular mechanisms have not been known.
Therefore, we examined the effects of very low density lipoprotein (VLDL) and 11-ketotestosterone
(11KT), a major androgen in some teleosts, on the accumulation of oil droplets into previtellogenic oocytes.
Although the production of glass eel in captivity has been succeeded in the Japanese eel, the egg quality
is unstable, and survival rates of the larvae are usually very low. To solve the problems, the techniques that
could produce fertilizable eggs and viable larvae in vitro may be useful, because the developmental process
of eggs and larvae can be controlled under completely artificial condition.
We have applied the basic knowledge from eel studies using the to the development of effective
techniques for the seed production in various fish species such as sturgeons. Our recent investigations have
focused on aspects of sturgeon biology, including the artificial control of reproduction in native sturgeons.
In this project, we investigated wild sturgeons, which are rarely caught off the coast of Hokkaido in Japan.
Achievements
1) Accumulation of oil droplets in previtellogenic oocytes of Japanese eel could be induced in vitro.
Co-treatment with VLDL and 11KT resulted in significant oil droplet accumulation. These data suggest that
lipids in oil droplets originate, at least in part, from VLDL, and that 11KT plays an important role in their
transfer and/or accumulation into oocytes (Endo et al., in preparation). This is the first report to indicate the
role of androgen in oogenesis.
18
2) We designed a system for the mass production of two forms of biologically active recombinant eel GTHs
(follicle stimulating hormone: FSH and luteinizing hormone: LH). We successfully produced bioactive
recombinant eel GTHs. Further optimization of the production method and of protein purification will
result in greater yields of the recombinant GTHs (Kazeto et al., in preparation).
3) We generated and characterized a polyclonal antiserum against eel steroidogenic enzymes including
P450 cholesterol side-chain cleavage (P450scc) using a recombinant protein as the antigen (Ijiri et al.,
2006).
4) We performed in vitro induction of final oocyte maturation and ovulation in the Japanese eel, and tried
artificial fertilization using the eggs obtained in vitro. We succeeded to produce eel larvae from eggs
obtained in vitro for the first time, although the fertility and hatchability were mostly low (Abe et al., in
preparation).
5) Fifty sturgeons were caught in a 15-year period and identified as kaluga, Huso dauricus, Japanese
sturgeon (mikado), Acipenser mikadoi, Amur sturgeon, A. schrenckii and kaluga-Amur sturgeon hybrid in a
ratio of 38:10:1:1, indicating that several sturgeon species are still occurring in Hokkaido (Omoto et al.,
2004).
6) Recently, we have succeeded in the artificial spawning of kaluga for the first time in Japan. The hatching
rate, however, was extremely low since the incubation water temperature may have been inappropriate
(Adachi et al., in preparation). In addition, we have confirmed by biopsy that it will be possible to induce
spawning in one of the female mikado in the next year.
Significance and Perspectives
It is essential to establish an in vitro culture system and to analyze the hormonal regulation of the
reproduction-related factors for the elucidation of the mechanism in eel ovarian development. The basic
information from eel studies will facilitate to improve techniques for the artificial control of the
reproduction in not only eel but also other important fish species. We hope that these studies will be applied
to the development of innovative techniques for the seed production such as an in vitro egg production or a
surrogate aquaculture.
Selected publication
Omoto N., Maebayashi M., Hara A., Adachi S. and Yamauchi K. (2004) Gonadal maturity in wild sturgeons,
Huso dauricus, Acipenser mikadoi and A. schrenckii near Hokkaido, Japan. Environment. Biol. Fish.,
70, 381-391.
Matsubara H., Kazeto Y., Ijiri S., Hirai T., Adachi S. and Yamauchi K. (2005) Serum steroid profiles in
artificially maturing female Japanese eel, Anguilla japonica. Aquaculture, 243, 393-402.
Ijiri S, Takei N, Kazeto Y, Todo T, Adachi S and Yamauchi K (2006) Changes in localization of cytochrome
P450 cholesterol side-chain cleavage (P450scc) in Japanese eel testis and ovary during gonadal
development. Gen Comp Endocrinol. 145, 75-83.
19
R02 Analyses on cellular and molecular mechanisms for
unreduced gametogenesis
Marine Bio-Manipulation Project (Mechanism Analysis on Gametogenesis/Surrogate Aquaculture)
Member
Katsutoshi Arai, Faculty of Fisheries Science, Hokkaido University (Team Leader)
Aquatic animals exhibit incomparably larger bio-diversity in reproductive systems: most aquatic animals
normally generate gametes (eggs and sperm) through meiotic divisions and reproduce bisexually, but some
special biotypes and/or populations of certain species reproduce unisexually in nature. If we disclose
cellular and molecular mechanisms responsible for atypical reproduction observed in certain aquatic
animals, the result can be applied for the development of innovative aquaculture biotechnology not only to
induce the gametogenesis without meiosis for efficient cloning, but also manipulate uniparental
development for all-female and/or all-male farming.
The loach, Misgurnus anguillicaudatus (Teleostei:Cobitidae) is a unique freshwater fish species
commonly inhabits in Japan and other areas in Eurasia. This species includes a small number of unisexual
form together with predominant bisexual form. Although the most loaches in Japan are a bisexually
reproducing diploid form with 2n=50 chromosomes, a cryptic clonal lineage was discovered in wild
population from the northern area of Hokkaido Island (Morishima et al., 2002). These clonal loaches are
diploid, but they produce unreduced diploid eggs genetically identical to somatic cells of the mother and
most diploid eggs develop without any genetic contribution of sperm donor. However, some eggs happen to
incorporate sperm nucleus and develop to triploid or diploid-triploid mosaic individuals. In general,
evolutionary origin of unreduced oogenesis and unisexual development has been considered to be a hybrid
between genetically distinct species or populations, which gives rise to a disruption of normal meiosis and
subsequently occurrence of atypical reproduction. Although the loach M. anguillicaudatus has been
regarded as a single species entity, the population genetic studies suggest the presence of cryptic species in
Japanese Misgurnus loach.
In this project, we investigate to disclose following seven important points using loach fish as model
animal: (1) the cytological mechanisms responsible for clonal unreduced diploid eggs, (2) the cytological
mechanisms responsible for unisexual development without genetic contribution of sperm, (3) the
cytological mechanisms for the occurrence of triploid and other unusual cytotypes, (4) the induction of
clonal males showing unreduced spermatogenesis to generate diploid spermatozoa with genetically
identical genotypes, (5) the cellular and molecular mechanisms responsible for unreduced spermatogenesis
in sex-reversed clonal males, (6) the population genetics using molecular markers to identify cryptic loach
20
species, and (7) the construction of genetic linkage map for identification of genetic factor for atypical
reproduction.
Achievements
1) Unreduced diploid eggs were determined to form by the system of “premeiotic endomitosis”, i.e.
chromosome doubling before entering meiosis without cytokinesis and subsequent quasi-normal two
divisions, based on observations of meiotic chromosomes and their behavior (Itono et al., 2006).
2) Unreduced eggs were determined to develop by “gynogenesis”, i.e. sperm-dependent parthenogenesis,
based on observations of failure of male pronucleus formation due to condensed sperm nucleus (Itono et al.,
2007).
3) Triploid and diploid-triploid mosaics appeared by probable reactivation of sperm nucleus in a blastomere
before or after cleavage, respectively, i.e. “delayed fertilization” (Itono et al., 2007).
4) Clonal males were successfully produced by sex-reversal with methyltestosterone administration and
they were confirmed to generate only diploid spermatozoa with clonal genotypes, based on experimental
breeding, flow-cytometry and molecular genetic analyses using microsatellites (Yoshikawa et al., 2007).
5) Premeiotic endomitosis is involved in diploid spermatogenesis and the stage when endomitosis occurs is
now examined using cell-sorter using stage-specific antibody and DNA content measurements.
6) Molecular phylogeny using sequences in the control region of mtDNA (about 950bp) disclosed that
Japanese Misgurnus loach includes at least two genetically distinct species (Morishima et al., 2007a).
7) Genetic linkage map including 27 linkage groups with centromeric position was constructed using about
150 microsatellite markers for further genetic analyses on atypical reproduction (Morishima et al., 2007b).
Our results show that unreduced oogenesis and spermatogenesis should be achieved by the breeding system
of “premeiotic endomitosis”. Now, the study to identify the stage when the endomitosis occurs is now
going underway. From the evolutionary viewpoint, the involvement of hybridization between genetically
distinct loaches is suggested for the occurrence of atypical reproduction. This means that it may be possible
to reproduce or reconstruct clonal lineage by artificial hybridizations. Clarification of cellular and
molecular mechanisms for unreduced gametogenesis and natural gynogenesis is a final goal.
Itono, M., Morishima, K., Fujimoto, T., Yamaha E, and Arai, K. (2006) Premeiotic endomitosis produces
diploid eggs in the natural clone loach, Misgurnus anguillicaudatus (Teleostei:Cobitidae).
J.Exp.Zool.305A:513-523
Itono, M., Okabayashi, N., Morishima, K., Fujimoto, T., Yoshikawa, H., Yamaha, E. and Arai, K. (2007)
Cytological mechanisms of gynogenesis and sperm incorporation in unreduced diploid eggs of the
clonal loach, Misgurnus anguillicaudatus (Teleostei:Cobitidae). J.Exp.Zool.307A:35-50
Yoshikawa, H., Morishima, K., Kusuda, S., Yamaha, E. and Arai, K. (2007) Diploid sperm produced by
arificially sex-reversed clone loaches. J.Exp.Zool.307A:75-83
21
R03
Studies on behavior of the cytoplasmic factors including
organelles during gametogenesis and zygote development
Marine Bio-Manipulation Project (Mechanism Analysis)
Member
Taizo Motomura, Field Science Center for Northern Biosphere, Hokkaido University (Team leader)
Takashi Todo, Faculty of Fisheries Science, Hokkaido University
Chikako Nagasato, Field Science Center for Northern Biosphere, Hokkaido University
Backgrounds, Objectives and Research Outline
After fertilization, the cytoplasmic factors,
centrioles as a part of microtubule
organizing center, mitochondria (and
chloroplasts in the case of photosynthetic
eukaryotic algae) in zygote are introduced
from egg (female gamete) and sperm (male
gamete), and these factors are equally or
unequally distributed into daughter cells
during the zygote development. When
considering on efficient seedling and
cultivation of marine organisms including fishes and algae, distribution mechanism of the cytoplasmic
factors would be important. Because inducing development of parthenogenetic unfertilized eggs and
triploid zygotes is now useful technique in fisheries science. If the distribution of these cytoplasmic factors
is disturbed, the zygote could not develop. Moreover, isogamy and anisogamy are observed in algae,
different from the case of animals, only oogamy. Therefore, it is necessary to examine the behavior of
organelles in each pattern of the sexual reproduction, isogamy, anisogamy and oogamy, and these
observations would also possess new idea on the evolution of the sexual reproduction of various organisms.
In this report, we will focus on the algal fertilization including the edible seaweeds.
Achievements
First, we have examined the cytoplasmic inheritance of cellular organelles (centrioles, mitochondria and
chloroplasts) using the brown algae in this project. Members in the brown algae like Sargassum, Laminaria,
Macrocystis, are quite important primary producers along the sea shore. Centrioles in zygotes are
universally derived from male gamete (sperm), independent of patterns of the sexual reproduction, isogamy,
anisogamy and oogamy. In isogamy and anisogamy, centrioles derived from male and female gametes exist
in zygotes just after fertilization, however, these from the female gamete selectively disappear. In oogamy,
egg does not have centrioles originally, and sperm introduce them into zygote. Therefore, the paternal
22
inheritance of centrioles are universal in the sexual reproduction in brown algae, and this phenomenon
might be common in the sexual reproduction of eukaryotic organisms, because we know that the paternal
inheritance of centrioles occurs in vertebrate and invertebrate animals including fishes and sea urchins. We
are now studying on this important mechanism of the paternal inheritance of centrioles because centrioles
have a crucial role as a part of centrosome which function as the mitotic spindle poles and MTOCs
(microtubule organizing centers).
Concerning the cytoplasmic inheritance of
mitochondria and chloroplasts of sperm, they were
specifically surrounded by ER in early zygote
development and finally digested in lysosomes in the
case of oogamy. This selective digestion process is
quite similar to the case of animal fertilization.
Labeling of ubiquitin molecules might be selectively
occurred on the surface of these sperm organelles. In
isogamy, chloroplast in the sporophyte cells which
have developed from zygotes are biparental, while it has recently become clear that mitochondria are
derived from the female gamete. We are now examining when the male mitochondrial DNA will disappear
by crossing experiment using haplotypes of mitochondrial DNA.
Adding to the fisheries science, marine organisms have been significant from a viewpoint on the basic
biology. As mentioned above, organelle inheritance of marine algae are strictly regulated after fertilization,
some of their regulation patterns are quite similar to it of other organisms and the other are characteristic on
the algae. Genome project of the brown alga, Ectocarpus siliculosus, has now proceeded in Roscoff Marine
Station in France, and we participate in this project as one of the core centers (analysis of the cell structure
of brown algae). Therefore, it will be possible to approach the regulation mechanism on the organelle
inheritance from the genome information.
Kato Y, Kogame K, Nagasato C and Motomura T (2006) Inheritance of mitochondrial and chloroplast
genomes in the isogamous brown alga Scytosiphon lomentaria (Phaeiphyceae). Phycol Res 54:65-71.
Motomura t (1994) Electron and immunofluorescence microscopy on the fertilization of Fucus distichus
(Fucales, Phaeophyceae). Protoplasma 178:97-110.
Nagasato C (2005) Behavior and function of paternally inherited centrioles in brown algal zygotes. J Plant
Res 118:361-370.
23
R04
Establishment of sterilization technology for host
individuals in germ-line chimera formation
Marine Bio-Manipulation Project (Surrogate Aquaculture/Mechanism Analysis on Gametogenesis)
Member
Katsutoshi Arai, Faculty of Fisheries Science, Hokkaido University (Team Leader)
Syuiti Abe, Faculty of Fisheries Science, Hokkaido University
Shinji Adachi, Faculty of Fisheries Science, Hokkaido University
Kouhei Yamauchi, Faculty of Fisheries Science, Hokkaido University
For efficient “surrogate production” through germ-line chimeras (see Project 05 in detail), transplanted
primordial germ cells (PGCs) from the donor should differentiate to functional gametes in the host which
should be sterilized so as not to generate gametes of itself. Because simultaneous formation of gametes
derived from both host and donor is inconvenient for exclusive production of gametes of target species with
high commercial values and/or important traits in germ-line chimeras.
To produce viable but sterile fishes, interspecific hybridization has been conducted in a large number of
combinations between fish species. However, reproductive performance of resultant hybrids is very
difficult to predict. Thus, experimental hybridization and subsequent evaluation are required to assess the
sterility or the fertility of hybrid. Triploidization, i.e. addition of extra set of chromosomes to original
diploid two sets of chromosomes, can be formed easily in fish by inhibition of the second polar body
release of fertilized eggs and resultant triploids are expected to be sterile due to a disruption of meiosis and
subsequent arrest of gametogenesis. The combination between triploidization and hybridization, i.e.
allo-triploidization (generation of triploid hybrid) is predicted to exhibit additional effects on sterility.
These chromosome manipulation techniques are considered to be applicable for establishment of sterile
host, but effects of such manipulations are different not only between species, but also between sexes and
verification of sterility should be carried out in each candidate species.
The other sterilization technology is a knock-down of the gene dead end(dnd) by morpholino antesense
oligonucleotids (MAO), which inhibits the process of translation and protein synthesis at ribosome and
subsequently gives rise to the lacking of germ-cells. To establish a molecular sterilization technology in the
loach, sequences of dnd were partially determined and MAO treatment was optimized.
In future application of surrogate aquaculture techniques to various aquaculture species, techniques of
artificial induction of hybrids and/or polyploids are also established and then performances of resultant
products are evaluated in barfin flounder (Mori et al., 2004, 2006; Saitoh et al.,2006: Lahrush et al., in
press), Japanese eel (Nomura et al., 2004, 2006), bester hybrid sturgeon (Omoto et al.,2005ab), masu
24
salmon (Sakao et al., 2006), amago salmon
(Yamaki et al., 2006), abalone (Okumura et
Sterile host induction
Sterile host evaluation
Loach
dead end MAO
Aquaculture species
（flatfish・eel・masu
salmon・abalone）
Hybrid
Auto-3n
Allo-3n
Induction of hybrids
and polyploids
al., 2007). To investigate differentiation of
gametes and gonadal cells, developmental
Evaluation of
reproductive
performance
stages were determined in the loach,
morphologically as well as
Application of sterile host for
efficient surrogate aquaculture
molecular-biologically (Fujimoto et al.,
2004, 2006). In addition to above
mentioned biological researches,
Social acceptance
of new technology
inter-disciplinary studies on social
Fig. Sterilization technology for host in germ-line chimeras
acceptance of new biotechnology are also
conducted (Shimomura, 2006). In this
project, following points have been studied, such as 1) sterilization technology using chromosome
manipulations including of 2n hybrid, auto-3n and allo-3n , 2) sterilization technology with dnd-MAO , 3)
induction of germ-line chimera using above-mentioned sterile hosts, 4) optimization of triploid induction
and performance evaluation, 5) determination of developmental stages in loach, and 6) inter-disciplinary
approach on social acceptance of bio-manipulation.
Achievements
1) Production and verification of sterility/semi-sterility in 2n and 3n (allo-3n) Misgurnus anguillicaudatus
female x M. mizolepis male hybrids and auto-3n M. anguillicaudatus.
2) Designing MAO based on dnd sequence and optimization of dnd-MAO treatment to sterilize loach.
3) Induction of germ-line chimeras using above-mentioned sterile host and verification of exclusive
production of donor-derived gametes.
4) Triploid induction by optimum treating conditions in aquaculture fish and invertebrate species and
evaluation of reproductive and other performances.
5) Basic developmental biology to determine stages for studies on gametic differentiation
6) Inter-disciplinary studies on social acceptance of biotechnology.
Sterilization technology based on chromosome manipulation and dnd-MAO treatment is established to
generate host individuals for the surrogate production. Efficient xenogenesis using germ-line chimeras can
be achieved by application of sterile host.
Sakao S, Fujimoto T, Kimura S, Yamaha E and Arai K. (2006) Drastic mortality in tetraploid induction
results from the elevation of ploidy in masu salmon Oncorhynchus masou. Aquculture 252: 147-160
Nomura K, Morishima K, Tanaka H, Unuma T, Okuzawa K, Ohta H and Arai K (2006)
Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis
using induced triploid families. Aquaculture 257：53-67
Fujimoto T, Kataoka T, Sakao S, Saito T, Yamaha E and Arai K (2006) Developmental stages and germ cell
lineage of the loach (Misgurnus anguillicaudatus). Zool. Sci. 23:977-989
25
R05
Establishment of several surrogate-aquaculture-techniques
Marine Bio-Manipulation Project (Surrogate Aquaculture)
Member
Etsuro Yamaha, Field Science Center for Northern Biosphere, Hokkaido University (Team leader)
Katsutoshi Arai, Faculty of Fisheries Science, Hokkaido University
Yoshiyuki Takahashi, Faculty of Veterinary Science, Hokkaido University
Shinji Adachi, Faculty of Fisheries Science, Hokkaido University
The term, surrogate, is used as surrogate mother in reproductive medicine. In the field of fertility treatment,
fertilized egg or early embryo is transplanted into uterus. Surrogate mother means a mother who bears a
child on behalf of another woman, from the implantation in her womb of a fertilized egg from the other
woman. Surrogate production is also used in animal husbandry. A birth of baby caw is controlled according
to the birth plan, transplanting fertilized egg. Fertilized eggs from beef cattle are used in transplantation,
because of the high market value. Surrogate production in aquaculture, this sort of technique will be used.
By this technology, the gametes with high market value will be produced in fish.
Surrogate production in teleost fish is achieved by inducing germ-line chimeras. To make germ-line
chimeras, the PGCs are isolated from donor embryo and transplanted into the host embryos. The hosts
become germ-line chimeras if the transplanted PGCs successively migrate to the genital anlage and
differentiate into functional gametes. In consequence, donor genotypes can be restored in the next
generation. The fish-seed production is expected to become more efficient by the realization of surrogate
production using germ-line chimeras between two different species whose biological properties are
different from each other. For example, the life cycle of the fish might be extremely shortened if a species
that has shorter life cycle is used as the surrogate parent and produces donor gametes. Moreover, if PGCs
from larvae distributed in nature are collected and reproduced through germ-line chimera, we might be able
to keep enormous genetic bio-diversity in stock seedlings for marine ranching.
In this project, following four points
have been studied, such as 1)
visualization of PGCs in several teleost
species during embryonic development,
2) isolation of visualized PGCs by
flow-cytometry, 3) induction of
germ-line chimera between different
species, and 4) cryopreservation of
Fig. Schematic illustration of surrogate aquaculture
embryo and dissociated embryonic cells
for efficient induction of germ-line
26
chimera.
Achievements
1) Visualization of PGC from somatic cells is achieved in several fish species, including goldfish, eel and
some flatfish, by injection of artificial GFP-zebrafish nos1 3’UTR mRNA to fertilized egg.
2) A cluster of visualized PGCs by GFP were sorted by flow cytometry in 10-15 somite stage embryos in
the zebrafish, goldfish and loach. Ninety eight percent of sorted cells were PGCs by testing in situ
hybridization as vasa probe. About a half of sorted PGCs showed viability and migration ability were
confirmed by in vivo transplantation of sorted cells into host embryos.
3) Complete germ-line replacement between two related teleost species the pearl danio (Danio
albolineatus) and the zebrafish (Danio rerio). A PGC of pearl danio embryo was isolated and transplanted
into blastula stage, zebrafish embryo in which development of its germ-line cells was prevented by an
antisense dead end morpholino oligonucleotide. Analyses of morphology and DNA showed that offspring
from natural spawning of these chimeras were of donor origin. Transplantation of single PGCs of goldfish
(Carassius auratus) and loach (Misgurnus anguillicaudatus) can similarly differentiate into sperm in
zebrafish host embryos.
4) Vitrification of whole-body embryo is ongoing experiment in zebrafish.
Our results show that xenogenesis is realistic and practical across species, genus and family barriers and
can be achieved by the transplantation of a single PGC from a donor species. It is interesting whether or not
differentiation of donor sperm is induced in host with more distant genetic relation than family. The
functional gamete production will be expected from single PGC that the genetic or ploidy properties are
artificially modified. For example, functional gametes may differentiate, when PGC from lethal hybrid is
cultured in viable host in vivo. Complete germ-line replacement will be also scientifically applicable to
analysis of differentiation and interaction of germ and somatic cells in gonad. Now we are studying in these
problems.
Otani, S., Kitauchi, T., Sakao, S., Saito, T., Maegawa, S., Inoue, K., Arai, K., and Yamaha, E (2005) The
formation of PGCs from the germline cells in spherical embryos derived from the blastodisc of 2-cell
embryos in goldfish, Carassius auratus. Int. J. Dev. Biol. 49: 843-850
Saito, T., Fujimoto, T., Maegawa, S., Inoue, K., Tanaka, M., Arai, K. and Yamaha, E (2006) Visualization of
primordial germ cells in vivo using GFP-nos1 3'UTR mRNA Int. J. Dev. Biol. 50: 691-700
Yamaha, E., Saito, T., Goto-Kazeto, R. and Arai, K (2007) Developmental biotechnology for aquaculture
with special reference to surrogate production in teleost fishes. J. Sea Res. 58:8-22
27
R06
The formation of a seaweed genome consortium in Asian Pacific regions
Marine Bio-Manipulation Project (Mechanism analysis ; Mechanism valuation)
Member
Naotsune Saga, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Kazuo Miyashita, Faculty of Fisheries Sciences, Hokkaido University
Takao Ojima, Faculty of Fisheries Sciences, Hokkaido University
Hiroyuki Mizuta, Faculty of Fisheries Sciences, Hokkaido University
Taizo Motomura, Field Science Center for Northern Biosphere, Hokkaido University
Post-genome analysis became to be the main stream in modern biology using eukaryotic organisms
including yeast, nematode, fly, mouse and human. In plants, genome analyses had been done for several
land plants, such as Arabidipsis thaliana and rice, and unicellular algae, such as Chlamydomonas and
diatoms. In addition, large scale-genome analysis of multicellular algae are recently promoted in a
world-wide level. It has already been organized some genome consortium in Europe and America, to which
highly amounts of national and/or international budget is invested. To date, some analyses have been
completed and others are nearly finished. Unfortunately, in the Asian-Pacific regions, organized promotion
for the genome analysis is largely behind in Asia in comparison with Europe and America, because only
some Japanese algal research groups try to progress genomic analysis with small grade, in which the
Division of Breeding Sciences of the Hokkaido University plays a central role. It is accepted that
multicellular seaweeds are important marine resources for human being in the 21st century, especially to
Asian-Pacific people with historical and area-governmental reasons.
The aim of the project 06 is to progress the building of “Asian-Pacific Genome Center” as a center of
education and research of the genome biology of marine macro-algae. In that case, it is important to
organize the genome consortium in Asian-Pacific regions according to the activity of education and
research by seaweed study groups belonging to the 21st Century COE Program.
We joined several seminars focused on genome researches to get information about current situation of
model plants. In addition, we joined annual meetings and symposiums of some academic societies to
recommend a marine red alga Porphyra yezoensis as a model plant with comments about the advantage and
future visions of its genome analysis. Since this project has produced the fine fruits and attained it’s own
object, it was finished in 2006.
Achievements
Recently, genome analysis is progressing using model organisms for the experimental materials to
elucidate the mechanisms of physiological regulations. These model organisms are E. coli, yeasts, fly,
28
nematode, mouse, rice, Arabidopsis, which have characteristics as small genome size, easy to culture,
small number of genes and/or easy to functional analysis. Since genome information is a powerful tool to
analyze various physiological events, it is important to determine model marine plants for progress the
genome analysis of multicellular seaweeds. According to such a background, we attended the First Model
Marine Organism Seminar, the seminar for genome analysis of a marine brown alga Ectocarpus organized
by Roscoff Marine Laboratory (France), etc. to collect information about current situation of genome
analysis using model organisms. In addition, we introduce Porphyra yezoensis as a model marine organism
with its advantages and respects at, for instance, the Seventh International Meeting of Marine
Biotechnology, the Third International Symposium of Industrial-Academia-Governmental Collaboration
for the Establishment of Marine Food Production in China and Japan by the 21st Century COE Program at
Udayana University. Moreover, based on information obtained, we made a proposal pamphlet for the
establishment of “Asia Genome Center” and opened our website.
Although the pioneers of seaweed genome analysis are Asian-Pacific researchers, the genomic study is
performed by only small groups using Porphyra yezoensis including a Porphyra group in the Hokkaido
University as a leader. Thus, the constitution for the study on seaweed genome biology is not well
organized in comparison with those in Europe and America. There is a consensus that multicellular
seaweeds as marine organisms are important marine resources for human being in the 21st century,
especially to Asian people with historical and area-governmental reasons. Therefore, it is quit important
output that we was able to built “Asian Genome Center” at the international marine city, Hakodate, as a
center of education and study of the genome biology of algae. In that case, the activity of education and
study by algal study groups belonging to the 21st Century COE Program promoted the organization of the
genome consortium in Asian area.
In conclusion, the project 06 has established the preliminary committee for “Asian–Pacific Genome
Center (presently Asian–Pacific Institute of Marine Biology) ” and the facility for this center was built by
an academic-industrial-governmental collaboration. Since this project has produced the fine fruits and
attained it’s own object, it was finished in 2006.
Preliminary committee of the Asian–Pacific Institute of Marine Biology (2006) Pamphlet for establishing
Asian–Pacific Institute of Marine Biology. Hakodate, Hokkaido University, p.6
29
R07 Research and development for the establishment of
research infrastructures of model marine plants and
genome sciences
Marine Bio-Manipulation Project (Mechanism analysis)
Member
The marine macro-red alga Porphyra yezoensis Ueda is one of commercially important organisms for Nori
breeding. This organism is currently recognized as a model organism for both basic and applied researches
of marine plants, because it has been established laboratory culture system, freeze stock method and the
catalog of expression sequencing tags (ESTs). However, since a fine genetic map with a number of
molecular makers and genetic transformation systems haven’t developed yet, much efforts is necessary to
progress molecular biology and genomics in P. yezoensis. The aim of the project 07 is therefore
methodological improvement of the genetic transformation and the production of mutants in P. yezoensis.
Achievements
Isolation of mutant strain of Porphyra yezoensis : Pigmentation mutants have been used as useful crossing
partners in genetic analysis of Porphyra. We have succeeded in inducing a novel brilliant green mutant
(MBG: Mutant Brilliant Green) by N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) treatment in the
organism. The contents of chlorophyll a (Chl. a) and phycoerythrin (PE) were significantly
lower in MBG than those in
previously
mutant
reported
(TU-2)
differences
in
but
green
the
phycocyanin
(PC) and carotenoid contents
were not significant between
two mutants. The ratios of
PE/Chl. a and PE/PC were also
different. Since MBG was
normally
reproducible
and
easily distinguished from wild
type, this mutant may serve as
a new useful crossing partner.
30
Development of the new technique for function analysis of Porphyra yezoensis gene : We have succeeded
in the development of a tool for studying Porphyra gene function using the yeast transformation system.
The gene encoding argininosuccinate synthetase (EC 6345) of the organism (PyARG1) was obtained by
PCR using an expressed sequence tag clone as a template, and subcloned into the yeast expression vector
pYES2. The gene was expressed when the vector harboring PyARG1 was introduced into an
ARG1-deficient strain of Saccharomyces cerevisiae, which resulted in successful complementation of the
mutant phenotype. The transformed cells could survive on a selective medium lacking arginine, and
transcripts of PyARG1 were detected by reverse transcription-PCR. A quantitative comparison showed that
the rescued mutant cells grew in the selective liquid medium with a minor reduction in growth rate relative
to wild-type cells. This is the first report in which the function of Porphyra gene has been directly
demonstrated. This technique will provide new opportunities for further investigations into the functions of
various genes in the present organism and other macroalgal species.
For mutant generation, we employed a chemical mutagenesis and obtained the color mutant which display
bright green color. Because of its unique color, it is possible that the mutant is useful for crossing with other
color mutants to analysis a lot of physiological events. We schedule the generation of morphological
mutants and study the plasticity of wild-type strain to obtain the basic knowledge useful for cross
experiments.
For the development of the genetic transformation system, we now try to select reporters as selection
makers and promoters to express these reporters for constructing transformation plasmids. We also develop
the functional analysis system of genes from the organism using yeasts. One successful example is a
functional complementation of yeast arg1 mutant, whose growth requires exogenous supply of arginine, by
heterogeneous expression of P. yezoensis AGR1 gene. However, establishment of the genetic transformation
system is now our current main subject.
Selected publications
K. Matsuyama-Serisawa, M. Yamamoto, M. Fujishita, H. Endo, Y. Serisawa, S. Tabata, S. Kuwano and N.
Saga (2007) DNA content of the cell nucleus in the macroalga, Porphyra yezoensis(Rhodophyta). Fish.
Sci. 73:738-740
H. Endo, S. Ootsuka, S. Fukuda, Y. Kitade and N. Saga (2006) Functional complementation of an arginine
auxotrophic yeast mutant by an argininosuccinate synthetase from Porphyra yezoensis(Rhodophyta). J.
Phycol. 42:1066-1071
R. Tomimatsu, M. Takahashi, H. Endo, Y. Kitade, H. Yasui and N. Saga (2006) Induction and
characterization of a brilliant green mutant in a marine red alga Porphyra yezoensis (Bangiales,
Rhodophyta). Fish. Genet. Breed. Sci. 36:43-47
31
R08
Studies on the molecular and cellular biology in the sexual
differentiation and gemetogenesis of the seaweeds
Marine Bio-Manipulation Project (In vitro gamete production ; Mechanism valuation)
Member
Seaweeds (green, red and brown algae), namely multicellular
macroscopic algae, are important primary producers on the sea
coast, and have a crucial role for marine fishes and animals as
foods and maintenance of the sea shore environment. Konbu
(Laminaria), Wakame (Undaria) and Nori (Porphyra) have
been familiar to Japanese people as traditional foods and
culture, and the characteristic polysaccharides of the cell walls
of seaweeds, like agar, carrageenan, alginate, and fucoidan are
paid attention as medicines and healthy foods. Therefore, when
considering the efficient and stable management and
production of these edible seaweeds, we must understand the
regulation of there complicated life cycles, especially on the
gametogenesis. In this project, we have investigated
gametogenesis of the red (Porphyra yezoensis) and brown
(Scytosiphon lomentaria) algae using the molecular and
cytological techniques.
Achievements
In Porphyra yezoenesis, ultrastructures of mitosis, cytokinesis
and cell wall structures were obtained using freezing fixation.
Compositions of the cell wall polusaccharides in Porphyra are
different between sporophyte and gametophyte generations,
namely cellulose and mannan. Cell wall of the sporophyte cell
is composed of fine fibrous material, while it of the gametophyte cell is amorphous one. In the red algae,
the pit plug is characteristically detected in the cell wall between two cells. The pit plug exists only in
sporophyte generation, not in gametophyte one, in Porphyra. Nuclear division and cytokinesis in Porphyra
are similar to ones of other red algae. Nuclear envelope remains through mitosis, except of the mitotic polar
32
region. Polar rings, which are characteristic in red algae and like centrioles in animal cells, are located at
both mitotic poles, and numerous spindle microtubules radiated towards chromosomes. Through this study,
it has become clear that polar rings behave as semi-conservative manner. Cytokinesis proceeds with
furrowing of the cell wall.
In the brown alga, the genome project of a model organism Ectocarpus siliculosus has now proceeded
in Roscoff Marine Station in France under cooperation with other laboratories including our lab, and the
analysis on the whole genome will be completed in this year. Based on this genome information,
proteomics analysis using isogamous male and female gametes in Scytosiphon lomentaria has started with
researchers in Korea and Germany, focusing on the sexual differentiation, pheromone synthesis in female
gametes and its receptor in male gametes.
As mentioned above, the algal cell wall polysaccharides, as well as cellulose, chitin, etc. of the terrestrial
plants, have a big potential for the human life because of their unique structures and function. The cell wall
components are different between sporophyte and gametophyte generation in Porphyra, which are clearly
shown by TEM observation in this study. Therefore, early development of conchospores and carpospores
(monospore) must be examined, focusing on the cell wall formation, because the former develop into
gametophyte and the later into sporophyte. For this purpose, cytochemical studies including specific lectins
and antibodies under TEM will be necessary. Molecular information and technique have now proceeded in
Porphyra, and detailed studies on the metabolic pathway on the cell wall synthesis will be clarified under
the molecular level. But unfortunately, genetic transformation has not been succeeded at present. This
technique will surely be crucial for the future study on the marine algae including Porphyra, and our group
will combine the genetic manipulation and the ultrastructures in near future.
H. Endo, S. Ootsuka, S. Fukuda, Y. Kitade and N. Saga (2006) Functional complementation of an arginine
auxotrophic yeast mutant by an argininosuccinate synthetase from Porphyra yezoensis(Rhodophyta). J.
Phycol. 42:1066-1071
K. Matsuyama-Serisawa, M. Yamamoto, M. Fujishita, H. Endo, Y. Serisawa, S. Tabata, S. Kuwano and N.
Saga (2007) DNA content of the cell nucleus in the macroalga, Porphyra yezoensis(Rhodophyta). Fish.
Sci. 73:738-740
C. Ueki, C. Nagasato, T. Motomura and N. Saga : Ultrastructure of cytokinesis and pit plug formation in
Porphyra yezoenesis. Phycologia (in press)
33
R09
Clone seeds
Marine Bio-Manipulation Project (Clone seeds)
Member
Takao Ojima, Faculty of Fisheries Sciences, Hokkaido University
There are two general approaches to achieving vegetative propagation of major aquacultural seaweeds
including Porphyra and Laminaria. One is the use of totipotency to achieve protoplast production. The
procedure of protoplast formation generally requires great skill and time. In addition, it is necessary to
establish the simple and efficient technique for decomposing the algal cell wall. Isolation and purification
of the polysaccharide degradation enzymes fitting to each algal cell wall will achieve this resolution. On
the other hand, an alternative approach is to produce asexual spores such as monospores in Porphyra. The
monospore production is useful as a seed source in cultivation and has been focused on as one of the
cultivation techniques used to propagate biomass on the shore. The artificial control of monospore
production is also powerful technique to acquire a large amount of clonal seedlings.
Achievements
Cell-wall degradation enzymes from abalone and their application for protoplast production:An
endo-β-mannasase was isolated from digestive fluid of Pacific abalone, Haliotis discus hannai, by
successive chromatographies on TOYPEARL CM-650M, hydroxyapatite, and TOYOPEARL HW50F. The
abalone mannanase (HdMan) showed a molecular mass of approximately 39,000Da on SDS-PAGE, and
exhibited an optimal pH and temperature of 7.5 and 45°C, respectively. In addition, HdMan could disperse
the fronds of a red alga Porphyra yezoeisis into cell masses consisting 10-20 cells that are available for cell
engineering of this alga (Fig.1).
Fig. 1 Cell masses
Fig. 2 Protoplast of L.
produced by digesting
japonica isolated by
with HdMan
recombinant HdAly
Culture condition for protoplast production in the fronds of a brown alga, Laminaria japonica, was also
studied. Artificial seawater containing 100U/ml HdAly, 0.5% cellulase Onozuka and 0.6M mannitol was
suitable as a medium for protoplast production. In addition, the culture with 50 strokes/min of shaking at
17°C for 7 hr in the medium could produce 7.2-9.0 x 105 of protoplast per 1g of frond. The utilization of
34
the recombinant HdAly, which is prepared with the baculovirus insect-cell expression system, is powerful
tool for stable and continuous production of protoplast of this alga (Fig.2).
Mass production of monospore in Porphyra by allantoin treatment:A dioecious red alga, Porphyra
pseudolinearis, which does not produce asexual spore such as monospore, is a high valuable Porphyra
plants. We tried to acquire regenerable free cells, which have the same capability of monospore, by
allantoin treatment. A large amount of free cells were obtained after culturing in PES medium with 10-2M
of allantoin at 20 °C and homogenizing gently in seawater. The free cells were divided into three types in
form, globular, ellipse and 2-celled. Free cells in all types could developed to be thallus. There were also
free cells developing to be conchosporangium-like and conchocelis-like forms. The thallus originated from
the free cells showed the same levels of growth rate to mother plants. In addition, the thallus grew to be
large size more than 50cm in blade length. This technique is useful to acquire free cells even in Porphyra
species that has no cycle through monospore, and will contribute to production the clonal seedlings (Fig.3).
Fig. 3 Mass production of regenerable
free cells from Porphyra pseudolinealis
fronds by allantoin treatment
These approaches for seedling production in the present studies will supply the safe and reliable seedlings
for their cultivation. In future, the isolation and purification of other cell wall digestion enzymes, such as
xylanase, cellulase and agarase, will be necessary for truly a useful technique in artificial seedling
production. Moreover, the elucidation of characteristics of clonal seedlings is also necessary to establish the
practical system of seedling productions.
S. Ootsuka, S. Fukuda, D. Shibata, N. Saga and T. Ojima (2006) Preperation of abalone enzymes capable of
cell wall degradation of marine alga Porphyra yezoensis (Bangiales, Rhodophyta). Aquaculture
Science, 54, 83-88 (in Japanese with English abstract)
S. Ootsuka, N. Saga, K. Suzuki, A. Inoue and T. Ojima (2006) Isolation and cloning of an endo-β
-1,4-mannanase from Pacific abalone Haliotis discus hannai. J. Biotechnol. 125:269-280
H. Suzuki, K. Suzuki, A. Inoue and T. Ojima (2006) A novel oligoalginate lyase from abalone, Haliotis
discus hannai, that release disaccharide from alginate polymer in an exolytic manner. Carbohydr. Res.,
341, 1809-1819
35
R10 Hard tissue research and application in aquatic animals
Member
Yasuaki Takagi, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Kazuhiro Ura, Faculty of Fisheries Sciences, Hokkaido University
(This is a collaborative project with National Institute for Material Science and with Tokyo Institute of Technology)
The purpose of the project is to give new commercial value to hard tissues of aquatic animals, which are
wastes of seafood industry at present, by integrating molecular and physiological knowledge on the
mechanism of formation of these mineral containing tissues. The priority research item is fish scales and
their collagen. Artificial collagenous matrices for tissue engineering, such as “cornea substitutes” and
“bone substitutes” are putative outputs of the project.
Specific function of our tissues frequently depends on the specific fibril alignment of the collagenous
matrix. For example, corneal stroma is composed of stratified layers of highly-oriented collagen fibrils. The
directions of the fibrils in a layer are identical, but those in different layers are orthogonal. Such
three-dimensional alignment of collagen fibrils gives transparency, flexibility and strength to the tissue.
Since cornea has limited ability of regeneration, the only medical treatment for injured cornea is
transplantation. Thus, fabrication of cornea substitutes is the emergent task. Artificial collagenous matrices
that have cornea-like fibril alignment may be the best suitable, but low regenerating ability of the tissue
makes it difficult to study the mechanism of collagen alignment. On the other hand, regulation of
mineralization of implanted tissue is another task. Bone substitutes must keep mineralized, whereas
substitutes for soft tissues must keep unmineralized. Fish scale is composed of two layers, a thin external
bony layer and a thick internal basal plate. The former is bone-like mineralized collagenous matrix,
whereas the latter is a cornea-like. Moreover, the scale has high regenerating ability. The cells that produce
regenerating scales are extremely activated. Therefore, the regenerating scale is a good biological model to
study cellular mechanism of fabricating bone- and cornea-like collagenous matrix.
Achievements
Identification of molecules regulating collagen alignment and mineralization: Goldfish regenerating
scales are the major material used. We hypothesized that the noncollagenous proteins in the bony layer and
the basal plate were different, and that the former regulates mineralization whereas the latter regulates
collagen alignment. By SDS-PAGE, we found that the bony layer was rich in EDTA-soluble proteins,
whereas the basal plate contained small amount of acetic acid-soluble proteins. At present, we have
identified a protein that might have mineralization-inducing activity from the bony layer.
Establishment of in vitro systems that produce cornea- or bone-like matrix: Our histological
observation revealed that Leibovitz’s L-15 medium containing 10% FBS with high oxygen tension in the
36
atmosphere could support growth of regenerating scales in the organ culture system. To further asses the
culture condition, molecular markers that reveal the state of cellular differentiation and activity are needed.
For this purpose, we have cloned cDNAs of BMP2 and Runx2, both of which are powerful inducer of
osteoblast differentiation in mammals. cDNAs of type I collagen α-subunits and SPARC (osteonectin),
which are the molecules actively synthesized by osteoblasts, have also cloned. Quantitative RT-PCR
(QPCR) systems of these molecules are now under the development. Cell culture system is also under the
development using L-15 supplemented with 10% FBS.
Regulation of denaturation temperature (DT) in fish collagen: DT is the point over which collagen
triple helix is unraveled. The unraveled collagen (gelatin) looses a function as materials for tissue
engineering. Our colleagues found that tilapia collagen had close but a little bit lower DT (35°C) than
mammalian one (38°C). Moreover, tilapias from different aquaculture farms had different collagen DT,
suggesting that fish can control DT depending on the environment. Mammalian collagen is a triple helix of
two α1 subunits and one α2 subunit [(α1)2α2], whereas fish collagen is consisted of mammalian type
(α1)2α2 and fish specific types α1α2α3 and α2(α3)2. Collagen DT is highly dependent on the number of
hydroxy proline residues in the molecule. Since numbers of hydroxy proline residues in α1-, α2-, and
α3-subunit are different, fish may control the number of the residues, hence collagen DT, by controlling the
composition of the subunits. To examine this hypothesis, precise changes in the expression levels of α1-,
α2-, and α3-subunit mRNAs in different physiological conditions are under investigation.
The risk of zoonotic infection, such as bovine spongiform encephalopathy, has induced replacement of
collagen source from mammalians to fish. However, the replacement in medical use has not been
progressed. The present project will induce the replacement, and will further add high value to the scale
collagen. We are now at the stage that molecular tools have been just developed to study the mechanisms
of collagen alignment and mineralization in the regenerating scales. We will make a strong effort for
clarifying mechanisms of cellular differentiation and activation during scale regeneration in order to
establish in vitro systems that produce cornea- or bone-like matrix.
Yoshikubo H, Suzuki N, Takemura K, Hoso M, Yashima S, Iwamuro S, Takagi Y, Tabata MJ and Hattori A
(2005) Osteoblastic activity and estrogenic response in the regenerating scale of goldfish, a good
model of osteogenesis. Life Sci., 76: 2699-2709
Ohira Y, Shimizu M, Ura K and Takagi Y (2007) Scale regeneration and calcification in the goldfish
Carassius auratus: quantitative and morphological process. Fish. Sci., 73: 46-54
Takagi Y and Ura K (2007) Teleost fish scales: a unique biological model for the fabrication of materials
for corneal stroma regeneration. J. Nanosci. Nanotech., 7: 757-762
37
R11
Physiology of marine invertebrates: a molecular approach
Member
Yasuaki Takagi, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Akihiko Hara, Faculty of Fisheries Sciences, Hokkaido University
Takashi Todo, Faculty of Fisheries Sciences, Hokkaido University
Kazuhiro Ura, Faculty of Fisheries Sciences, Hokkaido University
Wild stocks of commercially important marine invertebrate species, such as abalones, sea urchins and crabs,
are in the general trend of decline due to overfishing, environmental destruction etc. The objective of the
present project is to accumulate basic molecular and physiological knowledge on the physiology of
commercially important marine invertebrates in order to support development of efficient technologies for
stock enhancement and aquaculture.
The target species of the project is a sea urchin, Strongylocentrotus intermedius, the most commercially
important invertebrate species in Hokkaido area. Its edible part is gonad, in which major cellular elements
are germ cells and nutritive phagocytes (NPs). The NPs accumulate nutritive substance, which is mobilized
under malnutrition or on the way of gonadal maturation. The sea urchin gonad is, thus, the reproductive as
well as nutritive organ. Studies are now focused on digestion and absorption of nutrients in the digestive
tract, NP development, and germ cell differentiation and development.
Achievements
(1) Digestion and absorption of nutrients in the digestive tract: Although existence of some digestive
enzymes including proteases have biochemically indicated, molecular information is scarce. We have
purified a serine protease, subtilase, from digestive tracts of S. intermedius. The enzyme was the most
active protease in the digestive tract, having peak activities at pH 9 and 50°C. It retained more than 40% of
its maximum activity in the condition of sea urchin digestive tract (pH 6-7 at 10°C), suggesting its
importance in vivo. The western blot analysis and immunohistochemistry using a specific antibody
revealed that it was synthesized in some of the resorptive epithelial cells of the anterior half of the stomach,
secreted into the lumen, and worked in the spherules of foods in the lumen. It was also suggested that the
resorptive epithelial cells of the posterior stomach, intestine and rectum absorbed digested nutrients. As far
as we know, this is the first study that directly identified the functional differentiation of digestive tract of
sea urchin.
(2) NP development: The major component of the NP contents had been identified as the major yolk
protein (MYP) in S. purpuratus and Pseudocentrotus depressus. However, its function, production site,
regulation mechanism of production, accumulation and mobilization have not been clarified. We have
purified MYP and cloned its cDNA in S. intermedius. RT-PCR revealed that the MYP mRNA is expressed
38
in the digestive tract as well as in the gonad in both sexes, as observed in other sea urchin species,
suggesting that a part of MYP was produced in the digestive tract and transported to the NPs through
coelomic fluid. Immunohistochemical identification of MYP-producing cells in the digestive tract is under
investigation. We have also developed radial immunodiffusion (SRID) method to quantify MYP
concentration, and variations of MYP levels in the gonad and coelomic fluid during the gonadal
development are being investigated. Since MYP is contained in gonads of both sexes, and may function as
a nutrient substance as well as yolk protein, it is not a good indicator of female gonadal development. We
are now investigating female specific protein(s) from female gonads, which can be used as a marker to
study gonadal development.
(3) Germ cell differentiation and development: We have isolated and characterized vasa and nanos
homologous genes from S. intermedius in order to obtain specific markers of the germline. Two
vasa-related cDNAs, vasa and PL10, and a nanos cDNA were isolated from testicular cDNAs. The vasa
and PL10 cDNAs encode proteins of 779 and 774 amino acid residues, respectively, and both proteins
contain the eight conserved motifs of the DEAD-box protein family. The nanos cDNA encodes a protein of
232 amino acid residues, and the protein contains two CCHC Zn finger motifs, which are well conserved
in Nanos protein family. By RT-PCR, vasa and nanos mRNAs were detected only in ovary and testis,
whereas PL10 mRNA was widely expressed in various tissues. In situ hybridization revealed that signals
for vasa and nanos mRNAs were strong in oogonia and small oocytes, decreased as maturation proceeded,
and disappeared in maturing oocytes. Signal for PL10 mRNA was strong in oogonia and small oocytes and
weak in maturing oocytes. In testis, both vasa and PL10 mRNAs were detected in spermatogonia and/or
spermatocytes, but not in spermatids and spermatozoa. However, nanos mRNA was not detected in all
stages of spermatogenesis. These results suggest that the vasa and nanos genes are specifically expressed
in germ cells of the sea urchin, S. intermedius, and thus, should be useful as germ cell markers in future
studies on germ cell formation, differentiation and development in sea urchin.
Clarification of molecular mechanisms of gonadal development in commercially important echinoderms
have not been studied intensely. The present project will supply basic molecular and physiological data
required for development of efficient technologies of stock enhancement and aquaculture. Researches on
endocrine and/or neural control system of gonadal development is also required to be started.
Tanaka E, Shimizu M, Ura K and Ojima T Purification and immunohistochemical localization of
sbutilase in the digestive tract of sea urchin, Strongylocentrotus intermedius. submitted to Nippon
Suisan Gakkaishi, in Japanese.
39
R12
“Epidemic prevention” to avoid diseases spreading via
seeds
Food Safety Project (Epidemic prevention)
Member
Mamoru Yoshimizu, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Toyohiko Nishizawa, Faculty of Fisheries Sciences, Hokkaido University
Hisae Kasai, Faculty of Fisheries Sciences, Hokkaido University
Tomoko Shimizu, Graduate School of Fisheries Sciences, Hokkaido University
Mass mortalities due to infectious diseases have often occurred in larvae and juveniles reared in hatcheries.
Although control measures are strongly needed for sustainable aquaculture, vaccination could not be an
effective method for larvae because immunological defenses of larvae are at an early stage of development.
Water supplies for seed production and aquaculture often provide an efficient means for the
introduction and spread of infectious diseases. Furthermore, disinfection of wastewater before discharging
is necessary to avoid the pathogen contamination in the environment. In this project, we study to establish
control measures by water disinfection, brood-stock selection and utilization of antiviral substance
producing bacteria as probiotic.
Achievements
1. Typical treatment systems make use of high efficiency sand filters to clarify the water before treatment
with ultraviolet (UV) light or ozonization. Fish pathogens are divided into two groups based on their
sensitivity to UV and total residual oxidants (TROs) produced by ozonization of seawater.
Hypochlorite produced by electrolysis of seawater (salt water) showed bactericidal and virucidal
effects. This method can easily treat large volumes of water, and is suitable for disinfecting wastewater
before discharging.
2. Fish with and without infectious hamatopoietic necrosis history (one of the severe salmonid disease)
was distinguished from each other by detection of specific antibody against IHNV with the newly
established ELISA system. Thus, it was considered that the ELISA system could be useful for
selection of fish without IHN-history. Application of this system, selection of healthy brood-stock of
barfin flounder (Verasper moseri) and Japanese flounder (Paralichthys olivaceus) and water treatment
were conducted in several hatcheries and were able to avoid the disease outbreak (see Fig. 12).
3. Anti-viral substances-producing intestinal bacteria such as Vibrio spp. from Japanese flounder
(Paralichthys olivaceus) and barfin flounder
were isolated. When Japanese flounder larvae were fed
on rotifers with Vibrio sp. strain V-15 manipulated with the bacteria, the dominant intestinal microflora
was consisted by Vibrio spp. Also, flounder larvae fed with V. alginolyticus strain V-5 or V-23
40
manipulated rotifer showed anti-herpesvirus activities in the intestinal contents. Experimental
infection is needed for demonstration of protective capability contribution.
Wild
Hatchery
Diseased
Screening
Marking
???
Antibody detection
PCR
Disinfection
Inspection
Disinfection
Release
Disinfection
Healthy
Wastewater
Vaccination for aquaculture
Fig.12. Control measures for viral diseases of barfin flounder and Japanese flounder.
Electrolysis of seawater is one of the effective method to keep large volume of disinfected seawater. This
method will serve a useful purpose for seafood sanitation as well as for aquaculture. “Electrolysis of
seawater” and “utilizing antiviral substance producing bacteria as probiotic” have high originality and lead
in this field.
Nishizawa,T., S.Kinoshita, and M.Yoshimizu (2005) An approach for genogrouping of Japanese isolates of
aquabirnavirus in a new genogroup, VII, based on the VP2/NS junction region. J.gen.Virol., 86:
1973-1978.
Yoshimizu, M., T. Yoshinaka, S. Hatori and H. Kasai (2005) Survivability of fish pathogenic viruses in
environmental water, and inactivation of fish viruses. Bull. Fish.Res.Agen.,Supplement No.2: 47-54.
Shimizu, T., N.Yoshida, H.Kasai, and M.Yoshimizu (2005) Survival of koi herpesvirus (KHV) in
environmental water. Fish Pathol., 41: 153-157.
41
R13
Molecular population genetics of Pacific salmon for their
management and sustainable use
Food Security Project (Genetic controls)
Member
Syuiti Abe, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Noriko Azuma, Faculty of Fisheries Sciences, Hokkaido University
Moongeun Yoon, Graduate School of Fisheries Sciences, Hokkaido University
Effective enhancement of marine animals as food resources has so far been greatly hampered by their
underdeveloped genomics, because of lack of genetic basis for selection and breeding. Aquagenomics is
also prerequisite to develop a useful tool for proper handling of the populations of target species, e.g.,
conservation of natural populations by maintenance of genetic diversity and prevention of genetic
perturbation by cultured counterparts. Our goal is thus to make it possible to accomplish effective breeding
of marine animals and their genetic management and sustainable use by employing a variety of molecular
markers.
Current research activities associated with the COE program include the development of
mitochondrial (mt) and nuclear microsatellite (ms) DNA markers in commercially important Pacific
salmon, especially for chum salmon (Oncorhynchus keta) and masu salmon (O. masou), and their use for
the analyses of genetic variation and population structure in these species.
Achievements
1) Genetic variation and population structure of chum salmon inferred from the mtDNA and msDNA
analyses. More than 4,200 fish representing a total of 96 populations from Japan, Korea, Russia, and
North America were examined for nucleotide sequence variation in about 500 bp variable portion of the 5’
end of the mtDNA control region and allelic variation at four of eight developed msDNA loci. The
observed 32 haplotypes of three clades (A, B and C) were mostly associated with the geographic regions, in
that clade A and C haplotypes characterized Asian populations and clade B haplotypes distinguished North
American populations. Both the mtDNA haplotype diversity and the heterozygosity at examined msDNA
loci suggested a greater genetic variation in the populations of Japan than those of Russia and North
America. Various population genetic analyses with the mtDNA and msDNA data showed distinct genetic
differentiation of populations among and within the three regions and clear geographical structure in the
Pacific Rim.
2) Development of DNA microarray for rapid detection of mtDNA haplotypes in chum salmon.
An
oligonucleotide (DNA) microarray hybridization method was developed for rapid and accurate detection of
the previously identified nucleotide sequence variations in about 500 bp within the 5’ half of the mtDNA
control region of chum salmon. The method includes immobilization of synthesized oligonucleotides
42
containing respective polymorphic sites on a glass slide precoated with polycarbodiimide resin, a 2-hour
hybridization with DNA microarray of biotinylated polymerase chain reaction fragments spanning the 5’
variable portion followed by short washing, and visualization of hybridization signals by conventional
ABC method and scanner-assisted computation of signal intensity on a computer. The entire process of
hybridization and detection was completed within 4 hours. The resulting DNA microarray could detect all
of the single nucleotide mutations and therefore could be used to identify the sequence variations defining
all the mtDNA haplotypes of chum salmon as revealed previously by nucleotide sequence analysis.
In fact, identification of mtDNA haplotypes of more than 2,200 chum salmon collected at various
stations in the Bering Sea and North Pacific Ocean September 2002 and 2003 on-board ships and on land
showed non-random distribution of Asian and North American stocks of chum salmon in the Bering Sea
and North Pacific Ocean, in that the Japanese and Russian stocks were mainly distributed in the
northcentral and western Bering Sea, respectively, and that the North American stocks were abundant in the
North Pacific Ocean around the Aleutian Islands.
3) Genetic variation and population structure of masu salmon based on mitochondrial DNA sequence
analysis.
Nucleotide sequence variation in about 500bp sequence from the 5' end of the mtDNA control
region of masu salmon endemic to the Far East was analyzed for the genetic variation in more than 600 fish
from the 17 populations representing Japan (11) and Russia (6). Haplotype diversity based on the observed
51 haplotypes of nine clades suggested a similar level of genetic variation between the two regions. Various
population genetic analyses revealed distinctive genetic differentiation between populations among and
within regions, demonstrating strong structuring between the two geographic groups of populations.
The observed congruence between the results from mtDNA and msDNA analyses indicates that a battery of
two DNA markers will become useful for construction of a better baseline for genetic stock identification
of chum salmon in high seas. The preliminary analysis of mixed ocean samples suggests high potential of
the developed DNA microarray for stock analysis of mixed ocean aggregation of chum salmon.
The
results obtained in masu salmon also indicate the efficacy of mtDNA analysis in population genetics of this
species. Overall, molecular genetic markers under developed will become useful for phylogeographical
analysis and genetic management of Pacific salmon.
Sato S, Kojima H, Ando J, Ando H, Wilmot RL, Seeb LW, Efremov V, LeClair L, Buchholz W, Jin D-H,
Urawa S, Kaeriyama M, Urano A, and Abe S (2004) Genetic population structure of chum salmon in
the Pacific Rim inferred from mitochondrial DNA sequence variation. Environ. Biol. Fish. 69:
38-50
Moriya S, Urawa S, Suzuki O, Urano A, and Abe S (2005) DNA microarray for rapid detection of
mitochondrial DNA haplotypes of chum salmon. Marine Biotechnol. 6: 430-434.
Moriya S, Sato S, Azumaya T, Suzuki O, Urawa S, Urano A, and Abe S (2007) Genetic stock identification
of chum salmon in the Bering Sea and North Pacific Ocean using mitochondrial DNA microarray.
Marine Biotechnol. 9: 179-91
43
R14 Genetic variation and population structure of flatfish
Food Security Project (Genetic controls)
Member
Syuiti Abe, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Katsutoshi Arai, Faculty of Fisheries Sciences, Hokkaido University
Tetsuya Takatsu, Graduate School of Fisheries Sciences, Hokkaido University
Flatfish include many commercially important species, some of which are threatened for their resources by
overfishing for many years. However, effective enhancement program for flatfish has been greatly
hampered by the lack of genetic information including genome structure and function. Understanding of
genome structure is prerequisite to develop useful molecular markers for proper handling of the populations
of target species, e.g., conservation of natural populations by maintenance of genetic diversity and
prevention of genetic perturbation by cultured counterparts. Our goal is thus to make it possible to
accomplish effective breeding of flatfish and their genetic management and sustainable use by employing a
variety of DNA markers.
Our current research activities associated with the COE program include the analysis of genome
structure and function, molecular phylogeny, development of DNA markers, and molecular population
genetics using developed markers in flatfish.
Achievements
1) Molecular genetic and cytogenetic analyses of flatfish genome.
Basic karyotype information was
accumulated in nine species of flatfish including Japanese flounder (Paralichthys olivaceus), Barfin
flounder (Verasper moseri), Stone flounder (Kareius bicoloratus), Pointhead flounder (Hippoglossoides
pinetorum), Slime flounder (Microstomus achne), Right-eyed flounder (Pleuronectes punctatissimus),
Brown sole (P. herzensteini), Cresthead flounder (P. schrenki), and Marbled flounder (P. yokohamae).
Diploid chromosome number was 48 in all but Barfin flounder (46) and Pointhead flounder (44).
Location of rDNA sites and satellite DNA clones isolated from Japanese flounder genome was shown to
become a useful species-specific chromosome marker in these flatfish. In addition, long debatable
chromosome number and karyotpe information of Japanese flounder, i.e. 2n=46 or 48, was settled with fine
molecular cytogenetic techniques, and the karyotype was further refined for both morphological and
molecular characteristics in this species.
2) Development of DNA markers in flatfish.
Eight polymorphic loci of microsatellite (ms) DNA were
newly developed and characterized in Brown sole. Among these, four were found to be available for
parental assignment and population genetic analysis in this species. Actually, preliminary parental
assignment with these msDNA markers reached more than 90% to 100% in a couple of hatchery
populations of Brown sole. In these hatchery populations produced from limited number of parents,
44
apparent decrease in the genetic variability was commonly observed with the markers employed. With
cross-species amplification, seven of the eight msDNA markers were found to be available in Marbled
flounder, Right-eyed flounder and Stone flounder for population genetic analysis.
3) Molecular phylogeny and population genetics of flatfish.
Molecular phylogeny using nucleotide
sequence data of mitochondrial (mt) DNA cytochrome b gene in 17 flatfish species including the
aforementioned nine revealed clear monophyletic relationships among the examined species, in that
Marbled and Cresthead flounder were found to be placed in the same cluster with high statistic support,
sharing some haplotypes in both species. Further population genetic analysis using the same mtDNA gene
strongly suggested that Marbled and Cresthead flounder are not different species, probably separating
southern (Marbled) type and northern (Cresthead) type from common ancestor with dispersal along
Japanese Archipelago.
Nucleotide sequence analysis of the mtDNA control region in Brown sole revealed extremely high
molecular variation in nearly 500 fish collected from Japan and Korea. Population genetic analysis with the
same mtDNA region failed to show inter- and intraregional genetic differentiation, probably due to the
observed high molecular variability.
Understanding of genome structure will facilitate development of molecular markers and construction of
chromosome maps for effective breeding and management of flatfish resources. Refinement of Japanese
flounder karyotype with various molecular cytogenetic techniques will make it possible to construct a
physical map for validation of genetic linkage maps so far proposed in this species. Establishment of
phylogenetic relationships of flatfish is particularly important for identification of true fishery target
species. Overall, the current approach of molecular cytogenetics, phylogenetics and population genetics
will provide useful means for appropriate conservation, enhancement and breeding in flatfish.
Joh M, Takatsu T, Nakaya M, Higashitani T and Takahashi T (2005) Otolith microstructure and daily
increment validation of marbled sole Pseudopleuronectes yokohamae. Mar. Biol. 147: 59–69.
Fujiwara A, Fujiwara M, Nishida-Umehara C, Abe S, and Masaoka T (2006) Characterization of Japanese
flounder karyotype by chromosome bandings and fluorescence in situ hybridization with DNA
markers. Genetica, published online on December 23, 2006 (DOI 10.1007/s10709-006-9136-z)
Kim S, Morishima K and Arai K (2007) Isolation and characterization of polymorphic microsatellite DNA
marker in the brown sole, Pleuronectes herzenstini. Mol. Ecol. Notes 7:79-81
45
R15
Monitoring estrogenic contaminations in aquatic
environments using fish serum proteins
Food Security Project (Pollution evaluations)
Member
Akihiko Hara, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Evaluation of pollution on the wild and farm-raised fishery products, or the environments where these
animals have been raised, is important for the food security in the human life. Vitellogenin (Vg) and
choriogenin (Chg), major estrogen-inducible serum precursors for egg yolk and egg chorion, respectively,
have become important biomarkers for assessing the estrogenic potency of chemicals and exposure of
animals to estrogenic contaminants present in aquatic environments. Our research objectives were to
develop detection and evaluation systems for the estrogenic pollution using biomarkers (i.e., Vg and Chg).
To achieve this goal, our research addresses molecular and biochemical characterization of such
biomarkers and their derivatives, generation of their specific antisera and immunoassays, and field surveys
on the presence of biomarkers in the sera of wild and domestic fish populations, in conjunction with
exposure experiments in the laboratory environments (Figure 1).
Achievements
Our research performed from 2004 to 2006 include molecular and biochemical characterization of Vg and
Chg in various teleost species. Results indicated the presence of multiple Vg and Chg genes and proteins.
Various conventional or advanced immunoassays for the detection and quantification of fish Vg and Chg
proteins were developed, of which include single radial immunodiffusion (SRID), chemiluminescence
immunoassay (CLIA), and immunochromatography. Differential sensibility among the induction of dual
types of Vg and Chg proteins in response to estrogenic stimulation were confirmed mainly in medaka
(Oryzias latipes), a model species for the laboratory exposure experiments. Field surveys, which are
targeting to the presence of Vgs and Chgs in male fish inhabiting in various aquatic environments (rivers,
estuaries, coasts, and oceans) around Japan were performed; an abnormal circulation levels of these
biomarkers were evident in male fish caught from several urban aquatic environments, which the levels far
exceeded ones from fish inhabiting control (putative non-polluted) areas or domesticated fish that
exhibiting normal reproduction. Efficient procedures for the purification of multiple Vgs and Chgs, as well
as those for the development of Vg or Chg-type specific antisera and immunoassays, need to be developed
and adapted for various model species for the better evaluation of estrogenic pollution in aquatic
environments. Normal Vg levels (“the base line”) in wild male populations inhabiting in the control areas
or domesticated males exhibiting the normal reproduction needs to be verified for each target species to
interpret the significance of Vg presence in the “polluted” fish.
46
Presence of multiple Vg and Chg genes and proteins in teleosts, in addition to their differential
responsiveness to the estrogenic stimulation, led us to consider that the identification of Vg or Chg types is
necessary to accurately evaluate the result of Vg-based or Chg-based bioassays. Specific immunoassays
developed for our model species is a major accomplishment in an aquatic toxicology field and can also be
adopted for the evaluation of estrogenic pollution using other target species inhabiting in different
environments. Our preliminary field surveys revealed a significant degree of estrogenic activities in some
of urban aquatic areas in Japan and thus suggested a possible estrogenic pollution in these areas.
Developing a simple kit for the detection of Vg and Chg, as well as performing further field surveys and
laboratory tests, in a variety of fish species inhabiting at different aquatic areas will be the next step toward
our goal and will provide important information on the food security in human life.
Amano H, Fujita T, Hiramatsu N, Shimizu M, Sawaguchi S, Matsubara T, Kagawa H, Nagae M, Sullivan
CV and Hara A (2007) Egg yolk proteins in grey mullet (Mugil cephalus): purification and
classification of multiple lipovitellins and other vitellogenin-derived yolk proteins and molecular
cloning of the parent vitellogenin genes. J Exp Zool Part 307A: 324-341.
Fujita T, Fukada H, Shimizu M, Hiramatsu N, and Hara A (2005) Annual changes in serum levels of two
choriogenins and vitellogenin in masu salmon, Oncorhynchus masau. Comp Biochem Physiol,
141B:211-217.
Hiramatsu N, Cheek AO, Sullivan CV, Matsubara T and Hara A (2005) Vitellogenesis and endocrine
disruption. In Biochemistry and Molecular Biology of Fishes, Vol 6 (Mommsen TP, Moon TW eds),
Elsevier BV, pp. 431-471.
Figure 1. Schematic drawing of research
strategy for the Food Security Project
(Pollution evaluation). See text for details.
47
R16
Molecular Mechanisms for Safety of Lives
Food Safety Project (Pollution evaluations, Stress responses)
Member
Hiroyoshi Fujita (Team leader); Takeshi Saito; Atsushi Oda; Noriko Urushibara; Akira Nakayama;
Hiroshi Wakao; Shinzo Kimura-Abe; Kanae Sato; Tomoyuki Sasaki; Hokkaido University School of
Medicine
Many biological systems support our survivals in various environments. For example, bilirubin, the end-product
of heme catabolism, used to believed as one of body wastes as well as the pathogen of jaundice without any
physiological role. Last decade, our data and others indicated biological significance of bilirubin to protect lives
from active oxygen. Thus, heme metabolism became one of the important members in the field of protective
reactions against stress.
Since heme and/or hemoprotein seems to be capable of sensing environmental pollutants, we have been
searching heme-mediated response mechanism(s) to environmental stress to find out Bach1/Maf/Nrf2 system
for transcriptional activation. Since many environmental pollutants, such as lead, trichloroethykene, cadmium,
dioxin decrease cellular heme content through inhibition of heme synthesis and/or induction of heme
degradation (Fig. 1), binding activity of Bach1, negatively controlled by cytosolic heme, is increased. Hereby,
the investigation on roles as well as mechanisms of heme to monitor environmental pollutants will form the
basis for the biological monitoring
system of environmental pollutants.
Platelets is well known to change
Fig.1 Effects of 500,000 t of Environmental Pollutants
found in Teshima-island on Heme Metabolism
its shape in response to various
environmental hazards, the cells is also
one of the most suitable items to
Lead
Trichroloethylene
Transcriptional Regulation
ALAD
Bach1
SccCoA
Gly
Mitochondoria
ALAS
investigate effects of environmental
chemicals
on
lives.
To
Fe
Heme
environmental pollutants in platelets by
UROS
CPO
PROTOIX
FeCH
COPRO
PPO
UROD
Dioxine
lead
biological monitoring system using
elucidate signal tarnsduction routes of
PBGD
PB
G
URO
construct
platelets, we, therefore, decided to
AL
A
Cadmium
Arthenate
Dioxine
Degradation
HO-1
Biosynthesis
Bil IXα
means of proteome analyses.
Lives also rescue themselves from environmental hazards through immune system. NKT cells play the key
role to regulate immunity, thus our new member, Wakao, developed novel mouse clone cell system.
Achievements
1) Heme-mediated response to environment: Inducible isozyme of the key enzyme for heme degradation,
heme oxygenase-1 (HO-1), is well known to 32 kDa heat shock protein (HSP32) as well as 33 kDa oxygen
regulated protein (ORP33). In 2003, we reported that heme regulated ORP33 expression through Bach1 (J Biol
48
Chem, 278: 9125-33), indicating that the factor plays an important role in response to stress.
The other remarkable response to oxygen stress is erythroid differentiation. The most essential event in
erythroid differentiation is the accumulation of globin (J Biol Chem, 266: 17494-502, 1991; Blood, 83: 1662-7,
1994; Blood, 86: 940-8, 1995), however, exact mechanisms has not been well understood. Recent study
indicated that gene activation of β-globin is also regulated by Bach1 via cellular heme concentration (J Biol
Chem, 279: 5480-7, 2004). Oxygen derived house-keeping heme biosynthesis in decidual cells are also
suggested to be regulated by Bach1 (Congenital Anomalies, 47: 22-33, 2007).
2)
Signaling to change the shape of platelets in response to environmental stress: By proteome analyses, we
find a novel binding protein for the adaptor protein CrkL, and identified a new DOCK180 family protein whose
cDNA is 5.6 kb. This protein distributes in various tissues, and is suggested to contribute cell adhesion. After
adhesion of platelets, Rac activation is one of the most essential events, signaling molecules from Rac are,
however, unknown. We demonstrated that some of these molecules are three isoforms of WAVE protein and
WAVE regulating proteins, such as Abi-1, Sra-1 (Blood, 105: 3141-8, 2005). Furthermore, our data indicates
that IRSp53,with PIP3 controls WAVE2 to polymerize actin (J Cell Biol, 173: 571-85, 2006).
3) Environmental response in fish cells:
RTC-2 cells were treated with tributyltin compound (TBT) to
indicate activations of MAPK system, including ERK, JNK, p38, as well as MAPKK molecules, such as
MEK1/2, MKK4 and MKK3 in a dose- and time-dependent manner. Observed apoptosis in TBT examined cells
were protected either pretreatment with JNK inhibitor or with p38 MAPK inhibitor.
4) Study using ES cells: The immune cells, particularly, those that involved in the innate immune system are
pertinent to the environmental response of the host organism. Generation of the unlimited number of such cells
will quite useful not only for the detection of the environmental change but also for the regenerative medicine.
We have generated the ES cells by nuclear transfer and in inducing the lymphocytes.
Although ES cells technology is promising for the future regenerative medicine and for the cell-based
therapy, cells that are induced to differentiate into certain cell types or tissues cannot be used for patient due to
the immune rejection of the host. In this regard, it is desirable to customize the ES cells, i.e., establishing the
patient-specific ES cells. Nuclear transfer is a technique that circumvents such a problem. Our success in
inducing the lymphocytes from such ES cells paves the way for realizing medicine and cell based therapy.
Further works will be required to decipher mechanism underlying the host immune response against the
environmental change, and to accumulate the data relevant clinical application studies.
From 2004, our group has published 20 original articles closely relating to molecular mechanisms for safety
of lives. Nowadays, we are planning to construct human ES cell system, which will be start within this year. If
the human ES cells system planning will become available, the system enables us not only the monitoring
system for environmental hazards but also the screening system for marine products that are expected to contain
many useful seeds for us.
Selected publication (within 3 papers)
Tahara T et al.: Heme positively regulates the expression of beta-globin at the locus control region via the
transcriptional factor Bach1 in erythroid cells. J Biol Chem, 279: 5480-7, 2004.
Oda A et al.: WAVE/Scars in Platelets.
Blood, 105: 3141-8, 2005.
Wakao H et al.: A novel mouse model for iNKT cell study. J Immunol, in press, 2007.
49
R17
Member
Kazuo Miyashita, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Naotsune Saga, Faculty of Fisheries Sciences, Hokkaido University
Masashi Hosokawa, Faculty of Fisheries Sciences, Hokkaido University
Carotenoids are the most important pigments among those occurring in the nature and have been found to
afford several beneficial effects including cancer chemoprevention. Fucoxanthin is a xanthophyll that
contains two epoxy groups. Fucoxanthin is reported to contribute >10% of the estimated total production of
carotenoids. It is the major carotenoid that occurs in brown seaweeds (phaeophytes) which are the major
seaweeds among different classes occurring in nature. This project aims to elucidate fucoxanthin as an
important bioactive carotenoid with reference to its biofunctional and nutritional benefits it affords. An
effort is also made to find the good resources and the available methods for isolation and purification of
this marine carotenoid.
Achievements
Obesity is the excessive accumulation of body fat which is a major risk factor for diseases like
hypertension, diabetes arthritis and cardiovascular diseases. Uncoupling protein (UCP1) is a key molecule
for anti-obesity. UCP1 expression in brown adipose tissue (BAT) is known as a significant component of
whole body energy expenditure and its dysfunction contributes to the development of obesity. We have
found the anti-obesity effect of edible seaweed carotenoids, fucoxanthin, through protein gene expression
of UCP1 in white adipose tissue (WAT) not in BAT. In these studies, 0.05-0.2% fucoxanthin was fed to
animals. The weight of WAT was significantly lower in fucoxanthin-fed rats and mice than in control
group. In this case, UCP1 expression was found in WAT of fucoxanthin-fed mice, although there was little
expression in that of control mice. Expression of UCP1 mRNA was also found in WAT of fucoxanthin-fed
mice, but little expression in that of the control. It is certain that UCP1 is a key molecule for anti-obesity.
UCP1 usually expressed only in BAT, however, adult humans have very little BAT and most of fat is stored
in WAT. Considered as breakthrough discoveries for an ideal therapy of obesity, regulation of UCP1
expression in tissues other than BAT by food constituents would be important. From this viewpoint, the
anti-obesity effect of edible seaweed carotenoid, fucoxanthin, is very interesting, as its activity depends on
the protein and gene expressions of UCP1 in WAT. Fucoxanthin feeding also significantly reduced glucose
level of diabetic model mouse or normal mouse fed high fat diet. Fucoxanthin up-regulated glucose
transporter 4 mRNA expression of L6 myotubes, which express similar glucose transport kinetic profile to
that of adult muscle. Therefore, anti-diabetic effect of fucoxanthin would be mainly due to the glucose
transporter up-regulation. When fucoxanthin was fed to animals, DHA synthesis from 18:3n-3 in the liver
50
also increased. The induction of DHA in the liver would be due to the increase in the enzymatic activity for
DHA synthesis.
The multi-functionalities of fucoxanthin indicate that it is an important bioactive and health beneficial
carotenoid for preventing metabolic-syndrome.
Miyashita, K (2007) “Anti-obesity by marine lipids” In: Obesity Epidemiology, Pathophysiology, and
Prevention (Bagchi, D. and Preuss H.G. eds.), CRC Taylor & Francis, New York, pp. 463-475
Maeda H, Hosokawa M, Sashima T, Takahashi N, Kawada T, and Miyashita K (2006) Fucoxanthin and its
metabolite, fucoxanthinol, suppress sdipocyte differentiation in 3T3-L1 cells. Int. J. Mole. Med.,
18:147-152
Maeda, H., Hosokawa, M., Sashima, T., Funayama, K., and Myashita, K (2005) Fucoxanthin from edible
seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose
tissues. Biochem. Biophys. Res. Comm. 332:392-397
51
R18
Functional marine lipids and its utilization
Member
Kazuo Miyashita, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Masashi Hosokawa, Faculty of Fisheries Sciences, Hokkaido University
Keiichi Shimazaki, Research Faculty of Agriculture, Hokkaido University
Atsuo Kimura, Research Faculty of Agriculture, Hokkaido University
Aquatic plants have been found to possess conjugated fatty acids, with carbon chain length varying from 16
to 22 carbon atoms, as natural constituents in their lipids, both trienes and tetraenes occur in aquatic plant
lipids. Though many workers have been contributed to the information on the fatty acid composition of
marine lipids from different regions of the world, not much information is available on the occurrence of
these conjugated polyunsaturated fatty acids (PUFAs) in marine lipids. This project is mainly aimed to
research, involving biochemistry, physiology and nutrition, in relation to marine conjugated fatty acids.
Achievements
Conjugated PUFAs from marine origins showed cytotoxic effect on mouse tumor cell (SV-T2). These
conjugated trienoic acids were cytotoxic to SV-T2 cells below 20 μM. The same effect was observed in the
case of human monocytic leukemia cell (U-937). Generally, 9,11,13-conjugated linolenic acids (CLN), a
model conjugated PUFA, and all trans-CLN are more cytotoxic than 8,10,12-CLN and CLN containing cis
configuration, respectively. The higher cytotoxity of 9,11,13-CLN or all trans CLN isomers was partly due
to the different susceptibilities of these CLN isomers to lipid peroxidation. On the other hand, the inhibitory
effect of CLN on the growth of colon cancel cells was related to the regulation of peroxisome
proliferator-activated receptor (PPAR)γ. PPARγ ligands such as troglitazone and 15-d-prostaglandin (PG) J2
caused growth inhibition and induced apoptosis in cancer cells. CLN showed a higher ligand activity on
PPARγ than troglitazone. Bcl-2, GADD45, and p53 are known as an important molecular target in
apoptosis-inducing pathways. In Caco-2 cell treated with 9c,11t,13t-CLN, Bcl-2 expression was
down-regulated, while GADD45 and p53 expressions were up-regulated. Therefore, two possible
mechanisms of the anticarcinogenic activity of CLN can be hypothesized viz., induction of apoptosis via
lipid peroxidation and regulation of target gene and protein. CLN significantly reduced the frequency of
colonic aberrant crypt foci (ACF) in rat as a precursor of colon carcinogenesis. In this case, the
proliferating cell nuclear antigen (PCNA)-labeling indices in ACF and normal-appearing crypts also
decreased by dietary feeding of CLN. Furthermore, feeding of CLN enhanced apoptotic cells in ACF
without affecting the surrounding normal-appearing crypts. Chemopreventive ability of conjugated PUFAs
on rat colon cancer could be found in a long-term in vivo assay. Dietary administration of oil rich in CLN
(9c,11t,13t-18:3) significantly inhibited the development of colonic adenocarcinoma induced by
52
azoxymethane (AOM) in male F344 rats without causing any adverse effects. In addition, CLN intake
significantly reduced the multiplicities of colorectal carcinoma (number of carcinomas/rats) in rats.
Synthetic ligands for PPARα and PPARγ effectively inhibited AOM-induced ACF in rats. Therefore, it may
be possible that conjugated PUFAs suppress colon carcinogenesis by means of altering PPARγ expression
in colonic mucosa.
It has been well documented that conjugated linoleic acids (CLA) from daily products affords numerous
beneficial health effects. Similarly, as the enzymes responsible for the formation of CFAs of >18 carbon
chain length have been completely elucidated, they can as well be used for producing conjugated PUFAs
like conjugated EPA and conjugated DHA etc through the application of biotechnological techniques from
marine lipids. This coupled with the fact that most conjugated PUFAs especially CLN isomers afford the
same health benefits as CLA makes marine lipids as the potential sources for use in functional and
neutraceutical formulations.
Narayan B, Hosokawa M and Miyashita K (2006) “Occurrence of conjugated fatty acids in aquatic and
terrestrial plants and their physiological effects” In: Nutraceutical and Specialty Lipids and Their
Co-Products (Shahidi, F. ed.), CRC Taylor & Francis, New York, pp. 201-218
Yasui Y, Hosokawa M, Kohno H, Tanaka T, and Miyashita K (2006) Troglitazone and
9cis,11trans,13trans-conjugated linolenic acid: Comparison of their antiproliferative and
apoptosis-inducing effects on different colon cancer cell lines. Pharmacology, 52:220-225
Yasui Y, Hosokawa M, Kohno H, Tanaka T, and Miyashita K (2006) Comparison of growth inhibition and
apoptosis induction by troglitazone and 9cis, 11trans, 13trans-conjugated linolenic acid on different
colon cancer cell lines. Chemotherapy, 52:220-225
53
R19 “Safety controls” to ensure safety and reliability of seafoods
Food Safety Project (Safety controls)
Member
Mamoru Yoshimizu, Faculty of Fisheries Sciences, Hokkaido University (Team leader)
Hiroyoshi Fujita, Graduate School of Medicine, Hokkaido University
Sanae Ariga, Research Fuculty of Agriculture, Hokkaido University
Hisae Kasai, Faculty of Fisheries Sciences, Hokkaido University
Noriko Urushibara, Graduate School of Medicine, Hokkaido University
Tomoko Shimizu, Graduate School of Fisheries Sciences, Hokkaido University
“Food safety” is a matter of concern to the entire world. The marine products produced and processed in
Japan include products such as salmon roe and seafood delicacies that do not need heating processing. To
ensure the safety of such products, not only sanitation and hygiene control at the processing plant important,
but also controls to preserve freshness and quality control assurances of the raw material are critical. In this
project, we study to make system which covers every step from fishing through market auction, processing
to distribution for all marine products.
On the other hand, contamination of oyster with Vibrio parahaemolyticus or viruses like norovirus
(NV) remains a cause of food-borne illnesses. Elimination of these pathogens from oysters is economically
important for oyster traders and farmers. Our studies aim to establish effective depuration systems against
other food-borne pathogens.
Achievements
1. To control hygienic conditions at fishing ports, qualitative and quantitative bacterial observations have
to be considered. In the 29 fishing ports located at Hokkaido, Japan, the distribution and composition
of heterotrophic bacterial flora and coli-form bacteria were studied. Coli-form bacteria were isolated
inside and outside of the harbor throughout the year, and their counts increased as water temperature
increased.
2. A consistent basic hygiene and sanitation policy were constructed. The basic approach involves, at
every stage from fishing, prevention of products pathogens, maintaining temperatures below set levels,
thoroughly controlling the water and ice used, preventing damage to raw material, and minimal
processing time. Electrolyzed seawater was applied for sanitation and hygiene control and ensured rich
source of disinfected seawater (Fig.19).
3. Depuration of the contaminated oysters was performed in two kinds of seawater; electrolyzed seawater
and UV irradiated seawater. At chlorine concentration of 0.2~0.4 mg/L, electrolyzed seawater
completely eliminated Escherichia coli while remaining harmless to the oysters. Treatment with
electrolyzed seawater or UV irradiated seawater resulted in decrease of E. coli counts to 91 E. coli
54
MPN/100g or below detection limit.
4. Challenged with high hydrostatic pressure, FCV, norovirus surrogate, was almost completely
inactivated at 200 MPa for 5 min. Oysters separated from their shells under a pressure of 80 MPa for 5
min at 40 °C. Under these conditions, one to two-log. inactivation of FCV was observed.
Coliform positive
Seawater electrolyzer
applied for sanitation
and hygiene control
Fig.19. Application of electrolyzed seawater for sanitation and hygiene control.
Japan has long history for aquatic products industry. Our model will enhance the general sanitation and
hygiene level of fishing ports. Furthermore electrolysis of seawater will be one of good tool to reduce risks
of marine products by V. parahaemolyticus.
Yoshimizu, M. and H. Kasai (2005) Creating a “safe and worry-free” salmon products using a HACCP
system from fishing through processing to distribution. In 『 Proceedings of the 4th FiSCUP
Japan-Korea Joint Seminar on Fisheries Sciences』, pp.24-28, December 15-16, 2004, Sapporo, Japan.
Yoshimizu,M. and H.Kasai (2005) Disinfection of seawater for aquaculture by and electrolytic apparatus
and its application to fisheries sanitation. In 『 Proceedings of the JSPS-NRCT International
Symposium Joint Seminar 2004 – Productivity techniques and effective utilization of aquatic animal
resources into the new century』, pp. 269-277, December 19-21, 2005, Kasetsart University, Thailand.
55
Appendix 4
Publication List
Publication List 2004
page 57
page 68
page 84
56
Publication List, 2004
Original Articles
1.
Abe S, Sato S, Edpalina RR, Ando H, Kaeriyama M, Urawa S, and Urano A (2004) Stock identification of chum
salmon by mitochondrial DNA sequence analysis. NPAFC Tech Rep 5: 82-83.
2.
Adam AAG, Nagano M, Katagiri S, Takahashi Y (2004) In vitro culture of mouse preantral follicles using membrane
inserts and developmental competence of in vitro ovulated oocytes. J Reprod Dev 50: 579-586.
3.
Adam AAG, Takahashi Y (2004) Effects of oxygen tension in the gas atmosphere during in vitro maturation, in vitro
fertilization and in vitro culture on the efficiency of in vitro production of mouse embryos. Jpn J Vet Res 52: 77-84.
4.
Aoki M, Kurasaki M, Saito T, Seki S, Hosokawa T, Takahashi Y, Fujita H and Iwakuma T (2004) Nonylphenol
enhances apoptosis induced by serum deprivation in PC12 cells. Life Sciences, 74: 2301-2312.
5.
Atabay EC, Takahashi Y, Katagiri S, Nagano M, Koga A, Kanai Y (2004) Vitrification of bovine oocytes and its
application to interigeneric somatic cell nuclear transfer. Theriogenology 61: 15-23.
6.
Azuma N, Takahashi J, Higashi S and Sasaki M (2004) Characterization of microsatellite loci in Oecophylla
smaragdina. Mol Ecol Notes 4: 608-610.
7.
Azuma T, Takeda K, Doi T, Muto K, Akutsu M, Sawada M and Adachi S (2004) The influence of temperature on sex
determination in sockeye salmon Oncorhynchus nerka. Aquaculture, 234: 461-473.
8.
Baba K, Miyamoto A, and Hara A (2004) Relationship between the vitellin concentration in the hemolymph and
oocyte necroses during the annual reproduction cycle in the Japanese scallop, Mizuhopecten yessoensis. Invert
Reprod Develop, 45: 175-184.
9.
Edpalina RR, Yoon M, Urawa S, Kusuda S, Urano A and Abe S (2004) Genetic variation in wild and hatchery
populations of masu salmon (Oncorhynchus masou) inferred from mitochondrial DNA sequence analysis. Fish
Genet Breed Sci, 34: 37-44.
10.
Endo H, Takagi Y, Ozaki N, Kogure T and Watanabe T (2004) A crustacean Ca2+-binding protein with a
glutamate-rich sequence promotes CaCO3 crystallization. Biochem J, 384: 159-167.
11.
Fujimoto T, Kataoka T, Otani S, Saito T, Aita T, Yamaha E and Arai K (2004) Embryonic stages from cleavage to
gastrula in the loach Misgurnus anguillicaudatus. Zool Sci, 21: 747-755.
12.
Fujita T, Fukada H, Shimizu M, Hiramatsu N and Hara A (2004) Quantification of serum levels of precursors to
vitelline envelope proteins (choriogenins) and vitellogenin in estrogen treated masu salmon, Oncorhynchus masou.
Gen Comp Endocrinol, 136: 49-57.
13.
Fukada H, Ozaki Y, Pierce AL, Adachi S, Yamauchi K, Hara A, Swanson P, Dickhoff WW (2004) Salmon growth
hormone receptor: molecular cloning, ligand specificity, and response to fasting. Gen Comp Endocrinol, 139:
61-71.
14.
Hashimoto Y, Maegawa S, Nagai T, Yamaha E, Suzuki H, Yasuda K and Inoue K (2004) Requirement of localized
maternal factors for zebrafish germ cell formation. Dev Biol, 268: 152-161.
15.
Hendrayanti D, Denboh T, Ichimura T and Motomura T (2004) Molecular evidence of parallel origins of two
different parthenosporic lineages directly from heterothallic lineages in the Closterium moniliferum-ehrenbergii
(Charophyceae, Chlorophyta) species complex. Phycologia 43: 727-736.
16.
Hirayama H, Kageyama S, Moriyasu S, Sawai K, Onoe S, Takahashi Y, Katagiri S, Toen K, Watanabe K, Notomi T,
Yamashina H, Matsuzaki S and Minamihashi A (2004) Rapid sexing of bovine preimplantation embryos using
loop-mediated isothermal amplification. Theriogenology 62: 887-896.
17.
Horie S, Taniura K, Umino T, Nakagawa H and Arai K (2004) Performance of the progeny of natural tetraploid
loaches in long-term communal rearing experiments under a laboratory condition. Suisanzoshoku, 52: 91-98.
18.
Horie S, Taniura K, Umino T, Nakagawa H and Arai K (2004) Retarded growth of hexaploid loaches.
Suisanzoshoku, 52: 279-286.
19.
Hosokawa, M, Kudo M, Maeda H, Kohno H, Tanaka T and Miyashita K (2004) Fucoxanthin induces apoptosis and
enhances the antiproliferative effect of the PPARg ligand, troglitazone, on colon cancer cells. Biochim Biophys
Acta, 1675: 113-119.
57
20.
Inoue A, Ojima T, and Nishita K (2004) N-terminal modification and its effects on biochemical characteristics of
akazara scallop tropomyosin expressed in Escherichia coli. J Biochem, 136: 107-114.
21.
Katagiri S and Takahashi Y (2004) Changes in EGF concentrations during estrous cycle in bovine endometrium and
their alterations in repeat breeder cows. Theriogenology 62: 103-112.
22.
Kazeto Y, Place AR and Trant JM (2004) Effects of endocrine disrupting chemicals on the expression of CYP19
genes in zebrafish (Danio rerio) juvenile. Aquat Toxicol, 69: 25-34.
23.
Kikuchi T, Azuma N and Higashi S (2004) Grooming interaction and reproductive status among queens in
functionally polygynous colonies of the ant Myrmica kotokui (Hymenoptera : Formicidae). Sociobiology 44:
443-457.
24.
Kimura A, Lee J-H, Lee I-S, Park K-H, Chiba S, Kim D (2004) Two potent competitive inhibitors discriminating
a-glucosidase family I from family II. Carbohydr Res, 339: 1035-1040.
25.
Kim W-S, Ohashi M, Tanaka T, Kumura H, Kim G-Y, Kwon I-K, Goh J-Su and Shimazaki K (2004)
Growth-promoting effects of lactoferrin on L. acidophilus and Bifidobacterium spp. BioMetals, 17: 297-283.
26.
Kim W-S, Tanaka T, Shimazaki K (2004) Detection of binding-protein in Bifidobacterium bifidum Bb-11 by various
kinds of lactoferrin, Milchwissenschaft, 59.
27.
Kitade Y, Iitsuka O, Fukuda S and Saga N (2004) Porphyra yezoensis as a model plant for genome sciences. Jpn J
Physiol, 52: 129-131.
28.
Kobayashi H, Yoshida M, Maeda I and Miyashita K (2004) Proton NMR relaxation time of polyunsaturated fatty
acids in chloroform solutions and aqueous micelles. J Oleo Sci, 56: 105-108.
29.
Kohno H, Suzuki R, Yasui Y, Hosokawa M, Miyashita K and Tanaka T (2004) Pomegranate seed oil rich in
conjugated linolenic acid suppresses chemically induced colon carcinogenesis in rats. Cancer Sci, 95: 481-486.
30.
Kohno H, Yasui Y, Suzuki R, Hosokawa M, Miyashita K and Tanaka T (2004) Dietary seed oil rich in conjugated
linolenic acid from bitter melon inhibits azoxymethane-induced rat colon carcinogenesis through elevation of colonic
PPARg expression and alteration of lipid composition. Int J Cancer, 110: 896-901.
31.
Kono S, Kuwano K and Saga N (2004) Cryopreservation of the gametophytic cells of laminariales (phaeophyta) in
liquid nitrogen. J Phycol, 40: 606-610.
32.
Kubota M, Tsuji M, Nishimoto M, Wingchawalit J, Okuyama M, Mori H, Masui H, Surarit R, Svasti J, Kimura A
and Chiba S (2004) Localization of a-glucosidases I, II, and III in organs of Europian honeybee, Apis mellifera L.,
and origin of a-glucosidase in honey. Biosci Biotechnol Biochem, 68: 2346-2352.
33.
Kuroda TS, Maita H, Tabata T, Taira T, Kitaura H, Ariga H and Iguchi-Ariga SMM (2004) A novel nucleolar protein,
PAPA-1, induces growth arrest as a result of cell cycle arrest at the G1 phase. Gene 340: 83-98.
34.
Kusuda S, Koide N, Kawamura H, Teranishi T, Yamaha E and Arai K (2004) Cryopreservation of Sakhalin Taimen
Hucho perryi spermatozoa: effect of cryoprotectants on post-thaw fertility. Suisanzoshoku 52: 171-175.
35.
Kusuda S, Teranishi T, Koide N, Nagai T, Arai K and Yamaha E (2004) Pluripotency of cryopreserved blastomeres
of the goldfish. J Exp Zool, 301A: 131-138.
36.
Lee N-Y, Kawai K, Nakamura I, Tanaka T, Kumura H and Shimazaki K (2004) Susceptibilities against bovine
lactoferrin with microorganisms isolated from mastitic milk, J Vet Med Sci, 66: 1267-1269.
37.
Mahmoud BSM, Yamazaki K, Miyashita K, II-Shik S, Dong-Suk C and Suzuki T (2004) Bacterial microflora of carp
(Cyprius carpio) and its shelf-life extension by essential oil compounds. Food Micro, 21: 657-666.
38.
Mahmoud BSM, Yamazaki K, Miyashita K, II-Shik S, Dong-Suk C and Suzuki T (2004) Decontamination effect of
electrolyzed NaCl solutions on carp. Lett Appl Micro, 39: 169-173.
39.
Maita H, Kitaura, H, Keen, TJ, Inglehearn CF, Ariga H and Iguchi-Ariga SMM (2004) PAP-1, the mutated gene
underlying the RP9 form of dominant Retinitis pigmentosa, is a splicing factor. Exp Cell Res, 300: 283-296.
40.
Matsuura Y, Sasamoto Y, Sato K, Takahashi Y, Suzuki M and Ohtaishi N (2004) Monitoring ovarian cycle and
conception by fecal progesterone analysis in sika deer. Ecol Res, 19: 397-404.
41.
Matsuyama-Serisawa K, Yamazaki S, Kitade Y, Serisawa Y, Kuwano K and Saga N (2004) Tracing of systematic
characters of Bangiales (Rhodophyta) based on molecular phylogeny inferred from sequences of nuclear small
subunit rRNA genes. Aqua Cult Sci, 52: 185-198.
58
42.
Miyashita K (2004) Effects of flexibility and permeability of polyunsaturated fatty acid molecules on their oxidative
stability in aqueous systems. Lipid Technology Newsletter, 16: 197-202.
43.
Mohamed SMN, Nagano M, Katagiri S and Takahashi Y (2004) The use of nuclear transfer procedure for evaluation
of abattoir derived bovine oocytes. J Anim Vet Ad, 3: 800-803.
44.
Morishima K, Oshima K, Horie S, Fujimoto T, Yamaha E and Arai K (2004) Clonal diploid sperm of the
diploid-triploid mosaic loach, Misgurnus anguillicaudatus (Teleostei:Cobitidae). J Exp Zool, 301A: 502-511.
45.
Mori S, Yamazaki A, Matsuyama-Serisawa K, Fukuda S, Mizuta H and Saga N (2004) Effect of symbiotic bacteria
for growth of Porphyra yezoensis (Rhodophyta, Bangiales) in axenic culture. Aqua Cult Sci, 52: 239-244.
46.
Moriya S, Urano A, Urawa S, Suzuki O and Abe S (2004) Development of DNA microarray for rapid detection of
mitochondrial DNA haplotypes of chum salmon. NPAFC Tech Rep, 5: 28-30.
47.
Motomura T and Nagasato C (2004) The first spindle formation in brown algal zygotes. Hydrobiology 512: 171-176.
48.
Murayama E, Takagi Y and Nagasawa H (2004) Immunohistochemical localization of two matrix proteins in the
otolith and inner ear of the rainbow trout, Oncorhynchus mykiss: comparative aspects between the adult inner ear and
embryonic otocysts. Histochem Cell Biol, 121: 155-166.
49.
Nagasato C and Motomura T (2004) Destruction of maternal centrioles during fertilization of the brown alga,
Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae). Cell motility and the cytoskeleton, 59: 109-119.
50.
Nagasato C, Uemori C, Kato A and Motomura T (2004) Characterization of centrin genes from Ochromonas danica
(Chrysophyceae) and Scytosiphon lomentaria (Phaeophyceae). Phycological Research, 52: 266-272.
51.
Nakada N, Nyunoya H, Nakamura M, Hara A, Iguchi T and Takada H (2004) Identification of estrogenic compounds
in wastewater effluent. Environ Toxicol Chem, 23: 2807-2815.
52.
Nara M, Yumoto F, Nagata K, Tanokura M, Kagi H, Ojima T and Nishita K (2004) Fourier transform infrared
spectroscopic study on the binding Mg2+ to a mutant akazara scallop troponin C (E142Q). Biopolymer, 74: 77-81.
53.
Narayan B, Hosokawa M and Miyashita K (2004) Growth inhibition of human pro-myelocytic leukemia (HL-60)
cells by lipid extracts of marine alga Sargassum marginatum (Fucales, Phaeophyta) harvested off Goa (west coast of
India) with special reference to fatty acid composition. Indian Journal of Marine Sci, 33: 355-360.
54.
Narayan B, Kinami T, Miyashita K, Park Si.-B, Endo Y and Fujimoto K (2004) Occurrence of conjugated polyenoic
fatty acids in seaweeds from the Indian Ocean. Zeitschrift fur Naturforschung, 59c: 310-314.
55.
Nomura K, Nakajima J, Ohta H, Kagawa H, Tanaka H, Unuma T, Yamauchi K and Arai K (2004) Induction of
triploidy by heat shock in the Japanese eel, Anguilla japonica. Fish Sci, 70: 247-255.
56.
Ohkubo N, Andoh T, Mochida K, Adachi S, Hara A and Matsubara T (2004) Deduced primary structure of two forms
of vitellogenin in Japanese common goby (Acanthogobius flavimanus). Gen Comp Endocrinol, 137: 19-28.
57.
Okuyama M, Mori H, Chiba S, Kimura A (2004) Overexpression and characterization of two unknown proteins, YicI
and YihQ, originated from Escherichia coli. Pro Expres Purif, 37: 170-179.
58.
Omoto N, Maebayashi M, Hara A, Adachi S, Yamauchi K (2004) Gonadal maturity in wild sturgeons, Huso dauricus,
Acipenser mikadoi and A. schrenckii near Hokkaido, Japan. Environment Biol Fish, 70: 381-391.
59.
Rahman KM, Kumura H and Shimazaki K (2004) Growth promotional effects of bovine lactoferrin and its
hydrolysate on Bifidobacteria, Milk Science, 53 in press
60.
Saito T, Otani S, Fujimoto T, Suzuki T, Nakatsuji T, Arai K and Yamaha E (2004) The germ line lineage in ukigori,
Gymnogobius species (Gobiidae;Teleostei) during embryonic development. Intern J Dev Biol, 48: 1079-7085.
61.
Sakaguchi M, Sasamoto Y, Suzuki T, Takahashi Y and Yamada Y (2004) Postpartum ovarian dynamics and estrous
activity in lactating dairy cows. J Dairy Sci, 87: 2114-2121.
62.
Sano T, Nishimoto M, Saburi W, Kimura A, Yasuda H, Uchibatake M, Ohwada T and Masuda H (2004) Isolation
and characterization of cDNA encoding P-19.5 protein accumulated preferentially at early stage of carrot somatic
embryogenesis. Plant Sci, 167: 1211-1217.
63.
Sasamoto Y, Sakaguchi M, Nagano M, Katagiri S and Takahashi Y (2004) Follicular development after ovum
pick-up and fertilizability of retrived oocytes in postpartum dairy cattle. Jpn J Vet Res, 51: 151-159.
59
64.
Sato S, Kojima H, Ando J, Ando H, Wilmot RL, Seeb LW, Efremov V, LeClair L, Buchholz W, Jin D-H, Urawa S,
Kaeriyama M, Urano A and Abe S (2004) Genetic population structure of chum salmon in the Pacific Rim inferred
from mitochondrial DNA sequence variation. Environ Biol Fish, 69: 38-50.
65.
Sato S, Moriya S, Azumaya T, Suzuki O, Urawa S, Abe S and Urano A (2004) Genetic stock identification of chum
salmon in the central Bering Sea and adjacent North Pacific Ocean by DNA microarray during the early falls of 2002
and 2003. NPAFC Document No. 793, pp.1-21.
66.
Satou A, Hagio Y, Taira T, Iguchi-Ariga SMM and Ariga H (2004) Repression of the c-fms gene in fibroblast cells
by c-Myc-MM-1-TIF1b complex. FEBS Letters, 572: 211-215.
67.
Shimizu Y, Kitade Y and Saga N (2004) A nonradioactive whole-mount in situ hybridization protocol for Porphyra
(Rhodophyta) gametophytic germlings. J Appl Phycol, 16: 329-333.
68.
Shimomura K, Ootsuka S, Kitade Y, Kuwano K, Shin J-A and Saga N (2004) Phylogenetic analysis of bangiales
plants (Rhodophyta) using type II DNA topoisomerase gene. Fish. Genet Breed Sci, 33: 129-134.
69.
Suzuki R, Abe M and Miyashita K (2004) Comparative study on the autoxidation of TAG containing conjugated and
non-conjugated C18 polyunsaturated fatty acids. J Am Oil Chem Soc, 81: 563-569.
70.
Tahara T, Sun J, Nakanishi K, Yamamoto M, Mori H, Saito T, Fujita H, Igarashi K and Taketani S (2004) Heme
positively regulates the expression of beta-globin at the locus control region via the transcriptional factor Bach1 in
erythroid cells. J Biol Chem, 279: 5480-5487.
71.
Taira T, Iguchi-Ariga SMM and Ariga H (2004) Co-localization with DJ-1 is essential for the androgen receptor to
exert its transcription activity that has been impaired by androgen-antagonists Biol. Pharm Bull, 27: 574-577.
72.
Taira T, Saito Y, Niki T, Iguchi-Ariga SMM, Takahashi K and Ariga H (2004) DJ-1 plays a role in anti-oxidative
stress to prevent cell death. EMBO Rep, 5: 213-218.
73.
Takahashi-Niki K, Niki T, Taira T, Iguchi-Ariga SMM and Ariga H (2004) Reduced anti-oxidative stress activities of
DJ-1 mutants found in Parkinson's disease patients. Biochem Biophys Res Commun, 320: 389-397.
74.
Tanaka H, Maezawa Y, Ojima T and Nishita K (2004) Cloning and sequencing of cDNAs encoding walleye pollack
a-skeletal actin isoforms. Fisheries Sci, 70: 198-200.
75.
Tanaka M, Yamaha E and Arai K (2004) Survival capacity of haploid-diploid goldfish chimeras. J Exp Zool, 301A:
491-501.
76.
Tanaka T, Abe Y, Kim W-S, Kumura H, Nagasawa H, Igarashi I and Shimazaki K (2004) The detection of bovine
lactoferrin binding proteins on Trypanosoma brucei. J Vet Med Sci, 66: 619-625.
77.
Tanaka T, Morita H, Yoo Y-C, Kim W-S, Kumura H and Shimazaki K (2004) Detection of bovine lactoferrin
binding protein on Jurkat human lymphoblastic T cell line. J Vet Med Sci, 66: 865-869.
78.
Toyoshima K, Noguchi R, Hosokawa M, Fukunaga K, Nishiyama T, Takahashi R and Miyashita K (2004) Separation
of sardine oil without heating from surimi waste and its effect on lipid metabolism in rats. J Agric Food Chem, 52:
2372-2375.
79.
Tun M, Tanaka T, Kumura H, Akuzawa R and Shimazaki K (2004) Comparison of reactivity of polyclonal and
monoclonal antibodies against bovine lactoferrin and its fragmentsKhin Milk Science, 53: in press
80.
Wang Y, Suzuki A, Tanaka T, Haruto K and Shimazaki K (2004) Partial characterization of dextran-degrading
enzyme obtained from blue cheese, J Dairy Sci, 87: 1627-1633.
81.
Yamamoto T, Unno T, Watanabe Y, Yamamoto M, Okuyama M, Mori H, Chiba S, Kimura A (2004) Purification
and characterization of Acremonium implicatum a-glucosidase having high regioselectivety for a-1,3-glucosidic
linkage. Biochim Biophys Acta, 1700: 189-198, 2004.
82.
Yoon M, Brykov V, Varnavskaya N, Seeb LW, Urawa S and Abe S (2004) Mitochondrial DNA analysis of genetic
variation in the Pacific Rim populations of chum salmon. NPAFC Document No. 792, 1-25 p.
83.
Yoo Y-C, Shimazaki K and KimJ-B (2004) The role of ROS during bovine lactoferricin-induced apoptosis in THP-1
cells: regulation of caspase-3 activation and G1 arrest, Milk Science, 53: in press
84.
Yoshikawa S, Nagasato C, Makino Y, Murakami A, Kawai H, Ichimura T and Motomura T (2004) Nuclear histone
proteins of gametes in brown algae. Jpn J Phycology (Sorui), supplement issue 52: 123-127.
60
Other 16 papers published in Japanese domestic ｊournals.
Reviews and Books
1.
Suzuki K, Ojima T and Nishita K (2004) A GHF 9 cellulase gene from abalone Haliotis discus hannai. In
Biotechnology of Lignocellulose Degradation and Biomass Utilization (Ohmiya K, Sakka K, Karita S, Kimura T,
Sakka M and Onishi Y eds), pp407-411.
Other 13 review papers published in Japanese books and domestic journals
Others
1.
Amano H, Fujita T, Fujino H, Kagawa H, Yeo I-K and Hara A (2004) Characterization of three classes of
vitellogenins and lipovitellin in grey mullet. “Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of
Aquatic Animals”, November 2004, Cheju National University, Cheju, Korea, pp.20.
2.
Asanuma H, Ohashi H, Matsubara H, Ijiri S, Matsubara T, Adachi S and Yamauchi K (2004) 11-Ketotestosterone
potentiates estrogen-induced vitellogenin production in liver of Japanese eel (Anguilla japonica). Fish Physiol
Biochem, 28: 383-384.
3.
Hara A (2004) Fish vitellogenin as a biomarker for environmental estrogen. “Korea-Japan, Japan-Korea Joint
Meeting on Reproductive Biology of Aquatic Animals”, November 2004, Cheju National University, Cheju, Korea,
pp.37-39.
4.
Hatsuyama A, Hirai M, Hara A and Soyano K (2004) The influence of ethynylestradiol on the gonadal development
and vitellogenin production in the sexually undifferentiated juvenile grey mullet Mugil cephalus. “Korea-Japan,
Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals”, November 2004, Cheju National
University, Cheju, Korea, pp.21-22.
5.
Ijiri S, Takei N, Adachi S, and Yamauchi K (2004) Changes in immunolocalization of steroidogenic enzymes
(P450scc, P450c17, P450arom) in gonad of Japanese eel. Fish Physiol Biochem, 28: 209-210.
6.
Kasai H and Yoshimizu M (2004) Disinfection of seawater for aquaculture with electrolyzation and its application for
hygiene and sanitation of fishing port. “The Japan-Korea Joint Seminar on Fish Disease”, May 2004, Yosu University,
Yosu, Korea, pp.10-15.
7.
Kasai H, Nomura T and Yoshimizu M (2004) Surveillance and control of salmonid viruses of wild salmonid fish
returning to the northern part of Japan, from 1976 to 2002. “The Japan-Korea Joint Seminar on Fisheries Sciences”,
December 2003, Jinju-Tongyeong, Korea, pp.142-147.
8.
Kasai H, Sasaki K, Kumagai J and Yoshimizu M (2004) Creating a safe and worry-free salmon products using a
HACCP system from fishing through processing to distribution – the Shibetsu Town District HACCP Program model.
“The JSPS-NRCT International Symposium Joint Seminar 2004 – Management of Food Safety in Aquaculture and
HACCP”, December 2004, Bangkok, Thailand, pp.12-17.
9.
Nagae M, Kawasaki F, Katsiadaki I, Scott AP, Soyano K, Matsubara T, Ohkubo, N Hara A and Arizono K (2004)
Molecular approach to spiggin, the biomarker for environmental androgens, in the three-spined stickleback,
Gasterosteus aculeatus. “Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals”,
November 2004, Cheju National University, Cheju, Korea, pp.13.
10.
Nagano K, Kawasaki T, Ijiri S, Adachi S and Yamauchi K (2004) Effects of rearing water quality on expression of
grain corticotropin-releasing hormone and gonadal development in goldfish (Carassius auratus). Fish Physiol
Biochem, 28: 437-438.
11.
Nomura T and Yoshimizu M (2004) Disease problems of salmonid fish cultured in Japan.
Seminar on Fisheries Sciences”, December 2003, Jinju-Tongyeong, Korea, pp.132-135.
12.
Matsubara H, Lokman PM, Senaha A, Ijiri S, Kazeto Y, Kambegawa A, Hirai T, Young G, Adachi S and Yamauchi K
(2004) Synthesis and possible function of 11-ketotestosterone during oogenesis in eel (Anguilla spp.). Fish Physiol
61
“The Japan-Korea Joint
Biochem, 28: 353-354.
13.
Matsumoto S, Yamada H, Iwata M, Adachi S, Yamauchi K and Ueda H (2004) Changes in neurosteroid biosynthesis
during sexual maturation of lacustrine sockeye salmon. Fish Physiol Biochem, 28: 273-274.
14.
Mochida K, Fujii K, Kakuno A, Matsubara T, Ohkubo N, Adachi S and Yamauchi K (2004) Expression of ubiquitin
c-terminal hydrolase is regulated by estradiol-17β in testis and brain of the Japanese common goby. Fish Physiol
Biochem, 28: 435-436.
15.
Sakaki K, Matsubara H, Hirai T, Hara A and Nakamura M (2004) Effects ofbenzophenone on testicular differentiation
in carp, Cyprinus carpio. “Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals”,
November 2004, Cheju National University, Cheju, Korea, pp.24-25.
16.
Shin DH, Matsubara H, Kaneko S, Kotani T, Yamashita M, Adachi S and Yamauchi K (2004) Maturational factors as
indicators of egg quality in Japanese eel (Anguilla japonica). Fish Physiol Biochem, 28: 519-520.
17.
Wada T, Amano H, Fujita T and Hara A (2004) Serum levels of main vitellogenin (Vg1) in red lip mullet.
“Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals”, November 2004, Cheju
National University, Cheju, Korea, pp.19.
18.
Yang MH, Choe MK, Hara A and Yeo IK (2004) Expression of HSP 70 (heat shock protein) and cellular stress
response induction by endocrine disruptors in hepatocyte primary culture of the olive flounder, Paralichthys
olivaceus. “Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals”, November
2004, Cheju National University, Cheju, Korea, pp.34-35.
19.
Yoshimizu M (2004) Control strategy for viral disease of salmonid and flounder.
on Fish Disease”, May 2004, Yosu University, Yosu, Korea pp.1-9.
“The Japan-Korea Joint Seminar
Other 17 reports published in Japanese.
Presentations in the Scientific Meetings and Symposia
1.
Amano H, Fujita T, Fujino H, Kagawa H, Yeo I-K and Hara A (2004) Characterization of three classes of vitellogenins
and lipovitellin in grey mullet. Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals,
November 2004, Cheju National University, Cheju, Korea.
2.
Bando E, Shiokawa Y, Morishima K and Arai K (2004) Natural clones of the loach Misgurnus anguillicaudatus
(Teleostei:Cobitidae) identified by DNA markers. 29th International Conference on Animal Geneticds, September
2004, Meiji Univ. Tokyo, Japan.
3.
Edpalin, R., Sato S, Uraw, S, Brykov V, Urano A and Abe S (2004) Comparative population genetics of chum and masu
salmon using mitochondrial DNAsequence variation. NPAFC International Workshop on “BASIS 2004: Salmon and
Marine Ecosystems in the Bering Sea and Adjacent Waters”, October 2004, Sapporo Japan.
4.
Edpalina RR, Urawa S, Urano A and Abe S (2004) Genetic population structure of masu salmon inferred from
mitochondrial DNA sequence variation. Annual meeting of Japanese Society of Fisheries Science (JSFS)，April 2004，
Kagoshima Japan．
5.
Edpalina RR, Urawa S, Urano A and Abe S (2004) Population genetic analysis of masu salmon using mitochondrial
DNA sequence variation. The Sixth Japan-Korea, Korea-Japan Joint Symposium on Aquaculture, September 2004,
Hakodate, Japan.
6.
Freeman MA, Kasai H, Yoshimizu M and Ogawa K (2004) Ultrastructure and phylogeny of the X-cell protozoan
parasite associated with epidermal tumours in Pseudopleuronectes obscurus from Hokkaido, Japan. Annual Meeting
of Japanese Society of Fish Pathologists，Sept. 2004, Hakodate．
7.
Fujita T and Hara A (2004) Annual changes in serum levels of choriogenins and vitellogenin in masu salmon
(Oncorhynchus masou). The 6th Japn-Korea, Korea-Japan Joint Symposium on Aquaculture, September 2004,
Hakodate Harborview Hotel, Hakodate, Japan.
8.
Hamai H, Mori H, Mar SS, Chiba S and Kimura A (2004) Oxidation of Met398 correspomding to "Sugar Tongues" Tyr
of Barley a-amylase 1 leads to the low affinity towards starch granules in azuki bean a-amylase isoform 2. 22nd
62
International Carbohydrate Symposium, July 2004, Glasgow , UK.
9.
Hara A (2004) Fish vitellogenin as a biomarker for detection of estrogenic activity.
Symposium, August 2004, Queensland University, Queensland, Australia. (Invited)
Entox/QHSS One Day
10.
Hara A (2004) Aquaculture of Sakhalin taimen–vitellogenesis. One Day Symposium, Chinese Academy of Fisheries
Science，April 2004, Harbin，China. (Invited)
11.
Hara A (2004) Fish vitellogenesis.
China. (Invited)
12.
Hara A, Fukada H and Hiramatsu N (2004) Immnoassays of fish vitellogenin. Aquaculture 2004, March 2004, Hawaii
Convention Center, Honolulu, USA.
13.
Hara A, Matsubara T, Hiiramatsu N and Sullivan CV (2004) Fish vitellogenin as a biomarker for environmental
estrogens. Bilateral Seminar Italy and Japan, Physical and Chemical Impacts on Marine Organisms, November 2004,
Mierpearl-Ise Shima, Shima, Japan. (Invited)
14.
Hatsuyama A, Hirai M, Hara A and Soyano K (2004) The influence of ethynylestradiol on the gonadal development and
vitellogenin production in the sexually undifferentiated juvenile grey mullet Mugil cephalus. Korea-Japan,
Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals, November 2004, Cheju National University,
Cheju, Korea.
15.
Joh M, Takatsu T, Nakaya M and Sekiguchi M (2004) Annual change in growth pattern of marbled sole
Pseudopleuronectes yokohamae larvae in Hakodate Bay. The 6th Japan－Korea, Korea-Japan Joint Symposium on
Aquaculture, September 2004, Hakodate, Japan.
16.
Joh M, TakatsuT, Nakaya M, and Yoshida N (2004) The effect of contrasting environmental factors on the growth rate
of marbled sole Pseudopleuronectes yokohamae larvae. 2nd China － GLOBEC Symposiu, November 2004,
Hangzhou, China.
17.
Kasai H and Yoshimizu M (2004) Development of a vaccine injection machine for fish. The 4th Joint Seminar between
Japan and Korea by Core University Program on Fisheries Sciences. December 2004, Sapporo, Japan.
18.
Kasai H and Yoshimizu M (2004) Disinfection of seawater for aquaculture by electrolyzation and its application for
hygiene and sanitation of fishing port. The Japan-Korea Joint Seminar on Fish Diseases, May 2004, Yosu University.
19.
Kasai H, Sasaki K, Kumagai J and Yoshimizu M (2004) Creating a safe and worry-free salmon products using a
HACCP system from fishing through processing to distribution– the Shibetsu Town District HACCP Program model.
The JSPS-NRCT International Symposium – Management of Food Safety in Aquaculture and HACCP. December 2004,
Kasetsart University, Bangkok.
20.
Katagiri S and Takahashi Y (2004) Relationship between endometrial concentrations of epidermal growth factor (EGF)
and preimplantation embryo development in dairy cattle. 37th Annual Meeting Society for the Sutady of Reproduction,
Augasut 2004, Vancouver, Canada.
21.
Kawasaki T, Nagano K, Ijiri S, Todo T, Adachi S and Yamauchi K (2004) Effects of rearing water quality on expression
of brain corticotropin-releasing hormone and pituitary pro-opiomelanocortine in goldfish (Carassius auratus). The Sixth
Japan-Korea, Korea-Japan Joint Symposium on Aquaculture, September 2004, Hakodate, Japan.
22.
Kazeto Y, Goto-Kazeto R and Trant J.M. (2004) Molecular characterization of three forms of putative membrane-bound
progestin receptors in channel catfish, Ictalurus punctatus. 5th International symposium on Fish Endocrinology,
September 2004, Castellon, Spain.
23.
Kim Y-M and Kimura A (2004) Enzymatic Synthesis of Alkyl a-2-Deoxyglucoside by Alkyl Alcohol Resistant
a-Glucosidase, 4th Joint Symposium on Biological Resources and Environments between Yeungnam University and
Hokkaido university, February 2004, Sapporo, Japan.
24.
Kim Y-M, Okuyama M, Mori H, Funane K, Kobayashi M and Kimura A (2004) Dextranase from Paenibacillus sp.
Shows Cycloisomaltpdextrin Glucanotransferase Activity, Annual Meeting of Japan Society for Bioscience,
Biotechnology and Agrochemistry, March 2004, Hiroshima, Japan.
25.
Kim Y-M, Yamamoto E, Okuyama M, Mori H, Funane K, Kobayashi M, Chiba S and Kimura A (2004) Dextranase
from Paenibacillus sp. Shows Cycloisomaltpdextrin Glucanotransferase Activity, 22nd International Carbohydrate
Symposium, July 2004, Glasgow, UK.
26.
Kimura A (2004) Structure, function, protein engineering and protein-starch interaction of a-glucosidase. The 17th
FAOBMB Symposium, 2nd IUBMB Special Meeting, 7th A-IMBN Conference on "Genetics and Health on the 21st
One Day Symposium，Chinese Academy of Fisheries Science，April 2004, Peking,
63
Century", November 2004, Bangkok, Thailand.
(Invited)
27.
Kimura A, Okuyama M, Nakai H, Mori H and Chiba S (2004) Molecular analysis of a-glucosidase (GH-Family 31).
The Second Symposium on the Alpha-Amylase Family, October 2004, Smolenice, Slovakia. (Invited)
28.
Kishimura H, Ojima T, Hayashi K and Nishita K (2004) Characteristics of starfish phospholipase A2 mutants.
Asia-Pacific Marine Biotechnology Conference, Zhoushan, P. R. China.
29.
Kishioka C, Mori T, Saito S and Arai K (2004) Sex manipulation suing artificially induced gynogenesis and
marker-centromere mapping in barfin flounder (Verasper moseri). 29th International Conference on Animal Geneticds,
September 2004, Meiji Univ. Tokyo, Japan.
30.
Koba K, Akahoshi A, Tanaka K, Miyashita K , Iwata T, Kamagai T, Yamamoto T and Sugano M (2004) Dietary
Conjugated Linolenic Acid Modifies Body Fat Mass and Serum and Liver Lipid Levels in Rats, 95rd AOCS Annual
Meeting & Expo, May 2004, Kansas City, USA.
31.
Matsubara H, Hirai T, Amano H, Sekido S, Teramoto Y, Kawasaki T, Sato S, Hara A and Nakamura M (2004) Effect
of exogenous estrogenic substances on the expressions of three subtypes of vitellogenin genes in genetically controlled
male common carp, juveniles.7th Meeting of Japan Society of Endocrine Disrupters Research，Dec. 2004，Nagoya
International Conference Hall, Nagoya.
32.
Matsubara H, Hirai T, Teramoto Y, Satoh M, Hara A and Nakamura M (2004) Effect of estrogenic substances on
gametogenesis in generally controlled male common carp, Cyprinus carpio. Proceedings of the fifth congress of
AOSCE in conjunction with the annual meeting of JSCE, March 2004, Nara-ken public hall, Nara, Japan.
33.
Mine, Y., K. Wong and Miyashita K (2004) Purification and Characterization of Proteins with Antitumor Activity from
Bitter Melon (Momordica Charantia), 95rd AOCS Annual Meeting & Expo, May 2004, Kansas City, USA.
34.
Miyake M, Ura K and Takagi Y (2004) Change in concentrations of serum alkaline earth metals during seawater
adaptation of the masu salmon. The Sixth Japan-Korea, Korea-Japan Joint Symposium on Aquaculture, September
2004, Hokkaido Onuma International Seminar House, Nanae-cho, Hokkaido, Japan.
35.
Miyashita K (2004) Anti-obesity effect of seaweed carotenoid, fucoxanthin, and its application to nutraceuticals , The
Fifth International Conference and Exhibition on Nutraceuticals and Functional Conference, November 2004, San
Francisco, USA.
36.
Miyashita K (2004) Novel Fish Oils from Sardine Surimi, 5th Biennial European Conference on Fish Processing,
September 2004, Grimsby, UK. (Invited)
37.
Miyashita K (2004) Preparation of Fish and Algal Oils with Highly Nutritional Activities, 1st International Congress on
Functional Foods and Nutraceuticals (Key note speaker), April 2004, Antalya, Turkey.
38.
Miyashita K and Takenaka A (2004) Effects of Fatty Acyl Composition of Phospholipids on Their Antioxidant Actvities,
95rd AOCS Annual Meeting & Expo, May 2004, Kansas City, USA.
39.
Miyashita K, Araseki M and Kobayashi H (2004) Oxidative Stability of Polyunsaturated Fatty Acids in Liposome and
Cell Line Systems, 95rd AOCS Annual Meeting & Expo, May 2004, Kansas City, USA.(Invited)
40.
Moriya S, Sato S, Azumaya T, Suzuki O, Urano A and Abe S (2004) Genetic stock identification of chum salmon
(Oncorhynchus keta) in the Bering Sea by DNA microarray. NPAFC International Workshop on “BASIS 2004:
Salmon and Marine Ecosystems in the Bering Sea and Adjacent Waters”, October 2004, Sapporo, Japan.
41.
Motomura T and Nagasato C (2004) Algal cytoskeleton-Centrosomal or acentrosomal spindle formation. The 8th
Asia-Pacific Conference on Electron Microscopy, June 2004, Kanazawa, Japan.
42.
Nagae M, Kawasaki F, Katsiadaki I, A. P. Scott, Soyano K, Matsubara T, Ohkubo N, Hara A, and Arizono K (2004)
Molecular approach to spiggin, the biomarker for environmental androgens, in the three-spined stickleback,
Gasterosteus aculeatus. Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals,
43.
Nagae M, Kawasaki F, Soyano K, Katsiadaki I, A. P. Scott, Hara A and Arizono K (2004) Molecular cloning of two
types of spiggin cDNA in the three-spined stickleback, Gasterosteus aculeatus. The 6th Japn-Korea, Korea-Japan
Joint Symposium on Aquaculture, September 2004, Hakodate Harborview Hotel, Hakodate, Japan.
44.
Nagao M, Oku K, Sakurai M, Minami A, Kim Y.-M, Kimura A, Suzuki T, Mizuno K, Arakawa K, Fujikawa S and
Takezawa D (2004) Abscisic Acid Induced Increase of Oligosaccharides and Freezing Tolerance in Moss
Physcomitrella patens, 7th International Plant Cold Hardiness Seminar, July 2004, Sapporo, Japan.
64
6th
45.
Nagata T, Nozaki R, Takushima M, Todo T and Soyano K (2004) Development of hormone treatment techniques for
induction of maturation in teleost. The Sixth Japan-Korea, Korea-Japan Joint Symposium on Aquaculture, September
2004, Hakodate, Japan.
46.
Nakai H, Tanizawa S, Ito T, Matsubara K, Yamamoto T, Okuyama M, Mori H, Chiba S, Sano Y and Kimura A (2004)
Identification of Raw Starch Binding Site Existing in C-terminal Region of Plant a-Glucosidases, 22nd International
Carbohydrate Symposium, July 2004, Glasgow, UK.
47.
Nakai H, Tanizawa S, Saburi W, Son M, Ito T, Matsubara K, Yamamoto T, Okuyama M, Mori H, Chiba S, Sano Y and
Kimura A (2004) Identification of starch binding site existing in C-terminal region of plant a-glucosidases. Plant
Polysaccharide Workshop, July 2004, York, UK. （Best Poster Award）
48.
Nakano D, Ura K, Shimizu M and Takagi Y (2004) Morphological studies on the digestive canal of the sea urchin,
Strongylocentrotus intermedius. The Sixth Japan-Korea, Korea-Japan Joint Symposium on Aquaculture, September
2004, Hokkaido Onuma International Seminar House, Nanae-cho, Hokkaido, Japan.
49.
Nishizawa T, Higashi S and Yoshimizu M (2004) Nucleotide diversities of glycoprotein gene among Japanese isolates
of infectious hematopoietic necrosis virus (IHNV). The 6th International Symposium of Viruses of Lower Vertebrates,
September 2004, Hakodate, Japan.
50.
Nishizawa T, Kinoshita S and Yoshimizu M (2004) An approach for genogrouping of infectious pancreatic necrosis
viruses and other aquabirnaviruses based on VP2/NS junction region, A new genogroup VII with Japanese isolates. The
6th International Symposium of Viruses of Lower Vertebrates, September 2004, Hakodate, Japan.
51.
Nozaki R, Kadomura K, Yasumoto S, Todo T and Soyano K (2004) Semen cryopreservation in devil stinger, Inimicus
japonicus. The Sixth Japan-Korea, Korea-Japan Joint Symposium on Aquaculture, September 2004, Hakodate, Japan.
52.
Okuyama M, Mori H, Kimura A and Chiba S (2004) Two Functionally-unknown Proteins, YicI and YihQ, from
Escherichia coli Are a-Xylosidase and a-Glucosidase, 22nd International Carbohydrate Symposium, July 2004,
Glasgow, UK.
53.
Ose T, Kitamura M, Yao M, Okuyama M, Kimura A and Tanaka I (2004) Crystal structure of a-glucosidase homologue
from E. coli, The 8th SPring-8 Symposium, October 2004, Mikajuki-cho, Hyogo, Japan.
54.
Rahman Md. Morshedur, Kumura H and Shimazaki K (2004) Growth promotional effect of bovine lactoferrin and its
hydrolysate on Bifidobacteria, 1st Lactoferin Forum, October 2004, Tokyo, Japan.
55.
Saburi W, Mori H, Saito S, Okuyama M and Kimura A (2004) Streptococcus mutans Dextran Glucosidase Trp238 and
Short b→a loop 4 Are Responsible for Hydrolyzing Long-chain Substrate, Plant Polysaccharide Workshop, July 2004,
York, UK.
56.
Saga N (2004) Porphyra yezoensis as a model plant - The state and prospect. The 6th Asia-Pacific marine
biotechnology conference, Nov.28-Dec.1, (2004), Zhoushan, China.
57.
Sakaki K, Matsubara H, Hirai T, Hara A and Nakamura M (2004) Effects ofbenzophenone on testicular differentiation
in carp, Cyprinus carpio. Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals,
58.
Sato S, Yoon M.-G, Urawa S, Urano A and Abe S (2004) Mitochondrial DNA phylogeography of chum salmon in the
Pacific Rim. NPAFC International Workshop on “BASIS 2004: Salmon and Marine Ecosystems in the Bering Sea
and Adjacent Waters”, October 2004, Sapporo, Japan.
59.
Seeb J, Wilmot R, Urawa S, Abe S, Seeb LW and Smith C (2004) Standardization of DNA data for BASIS studies.
NPAFC International Workshop on “BASIS 2004: Salmon and Marine Ecosystems in the Bering Sea and Adjacent
Waters”, October 2004, Sapporo, Japan.
60.
Shimizu K, Senaha A, Kimura G, Matsubara H, Todo T, Adachi S and Yamauchi K (2004) Effects of androgen on early
oocyte growth in Japanese eel (Anguilla japonica). The Sixth Japan-Korea, Korea-Japan Joint Symposium on
61.
Shimizu K, Senaha A, Kimura G, Matsubara H, Todo T, Adachi S and Yamauchi K (2004) Induction of early oocyte
growth by the oral administration of androgen in Japanese eel Anguilla japonica. 7th Asian Fisheries Forum 04,
December 2004, Penang, Malaysia.
62.
Shin D-H, Ishihara M, Park C-W, Matsubara H, Kaneko S, Kotani T, Yamashita M, Todo T, Adachi S and Yamauchi K
(2004) Relationship between egg quality and maturity of ovulated egg in Japanese eel Anguilla japonica. 7th Asian
Fisheries Forum 04, December 2004, Penang, Malaysia.
65
63.
Shin D-H, Ishihara M, Park C-W, Matsubara H, Kaneko S, Kotani T, Yamashita M, Todo T, Adachi S and Yamauchi K
(2004) Maturity of ovulated egg in artificially matured Japanese eel Anguilla japonica. The Sixth Japan-Korea,
Korea-Japan Joint Symposium on Aquaculture, September 2004, Hakodate, Japan.
64.
Soyano K and Hara A (2004) Estrogenic contamination in teleosts collected in Japanese coastal waters. Bilateral
Seminar Italy and Japan, Physical and Chemical Impacts on Marine Organisms, Mierpearl-Ise Shima, November 2004,
Shima, Japan. (Invited)
65.
Suzuki R, Kohno H, Sugie S, Yasui Y, Hosokawa M, Miyashita K and Tanaka T (2004) Dietary Pomegranate Seed Oil
Rich in Conjugated Linolenic Acid Inhibits Azoxymethane-induced Carcinogenesis in Rats, 95rd AOCS Annual
Meeting & Expo , May 2004, Kansas City, USA. (Invited)
66.
Takahashi Y (2004) Cryopreservation of bovine oocytes and embryos. 7th World Buffalo Congress, October 2004,
Manila, Philippines.
67.
Takahashi Y (2004) Prospects of water buffalo cloning using intra- and inter-species nuclear transfer. 7th World Buffalo
Congress, October 2004, Manila, Philippines.
68.
Takushima M, Tanaka H, Matsumoto T, Todo T, Nakamura M, Soyano K (2004) Oocyte development and spawning
cycles in captive honeycomb grouper , Epinephelus merra. The Sixth Japan-Korea, Korea-Japan Joint Symposium on
69.
Tanaka H, Takushima M, Nakamura M, Todo T and Soyano K (2004) Induction of oocyte development by steroids and
LHRHa in immature honeycomb grouper (Epinephelus merra). The Sixth Japan-Korea, Korea-Japan Joint Symposium
on Aquaculture, September 2004, Hakodate, Japan.
70.
Toyoshima K, Noguchi R, Hosokawa M, Takahashi R and Miyashita K (2004) Separation of Sardine Oil without
Heating Process from Surimi Waste and Its Effect on Lipid Metabolism in Rats, 95rd AOCS Annual Meeting & Expo,
May 2004, Kansas City, USA. (Invited)
71.
Ura K, Shimizu M and Takagi Y (2004) Studies on the biology of the sea urchin for aquaculture. Korea-Japan,
Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals, November 2004, Cheju National University,
Jeju, Korea.
72.
Wada T, Amano H and Hara A (2004) Immunochemical studies on vitellogenin of red lip and grey mullet. 5th MRI
(Marine Research Institute) Symposium，March 2004，Seikai National Fisheries Research Institute, fisheries Research
Agency，Nagasaki．
73.
Wada T, Amano H, Fujita T and Hara A (2004) Serum levels of main vitellogenin (Vg1) in red lip mullet.
Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals, November 2004, Cheju
National University, Cheju, Korea.
74.
Wongchawalit J, Yamamoto T, Saji O, Okuyama M, Mori H, Chiba S and Kimura A (2004) Molecular Cloning of Two
a-Glucosidase Isozyme cDNAs from Japanese Honeybee, Apis cerana Japonica, Annual Meeting of the Japanese
Society of Applied Glycoscience, September 2004, Kagoshima, Japan.
75.
Yamamoto S, Suzuki H, Suzuki K and Ojima T (2004) Production of oligoalginates by the digestion of Laminaria with
abalone alginate lyase. 6th Asia-Pacific Marine Biotechnology Conference, Zhoushan, P. R. China.
76.
Yang MH, Choe MK, Hara A and I-K. Yeo (2004) Expression of HSP 70 (heat shock protein) and cellular stress
response induction by endocrine disruptors in hepatocyte primary culture of the olive flounder, Paralichthys olivaceus.
Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals, November 2004, Cheju
National University, Cheju, Korea.
77.
Yasui Y, Suzuki R, Hosokawa M, Kohno H, Tanaka T and Miyashita K (2004) Chemopreventive Effect of Bitter Gourd
Seed Oil Rich in Conjugated Linolenic Acid, 95rd AOCS Annual Meeting & Expo, May 2004, Kansas City, USA.
(Invited)
78.
Yoon M, Urawa S and Abe S (2004) Mitochondrial DNA analysis of population structure of chum salmon in the Pacific
Rim. The Sixth Japan-Korea, Korea-Japan Joint Symposium on Aquaculture, September 2004, Hakodate, Japan.
79.
Yoshida N, Takatsu T, Nakaya M, Joh M, Kimura O, Kobayashi N and Shimizu S (2004) Catch efficiencies of the
Hirota's sledge net for juvenile maebled sole and crangonid shrimp. 2nd China－GLOBEC Symposiu, November 2004,
Hangzhou, China.
80.
Yoshida Y, Ura K, Shimizu M, Sakurai Y and Takagi Y (2004) Development of the statocyst and statolith in the
Japanese common squid, Tadarodes pacifics: ultrastructual observations. The Sixth Japan-Korea, Korea-Japan Joint
66
Symposium on Aquaculture, September 2004,
Hokkaido, Japan. Poster presentation.
Hokkaido Onuma International Seminar House
Nanae-cho,
81.
Yoshimizu M (2004) Control strategy for viral diseases of salmonids and flounder. The Japan-Korea Joint Seminar on
Fish Diseases, May 2004, Yosu University.
82.
Yoshimizu M and Kasai H (2004) Creating a “safe and worry-free” salmon products using a HACCP system from
fishing through processing to distribution. The 4th Joint Seminar between Japan and Korea by Core University Program
on Fisheries Sciences, December 2004, Sapporo, Japan.
83.
Yoshimizu M, Yoshinaka T, Hatori S and Kasai H (2004) Survivability of fish pathogenic viruses in environmental
water, and inactivation of fish viruses. International Symposium on Koi Herpesvirus Disease – Strategy for Koi
Herpsvirus Disease Control, March 2004, Yokohama, Japan.
84.
Yung-Choon Yoo and Shimazaki K (2004) ROS elicits caspase-3 activation and G1 arrest during apoptosis induced by
bovine lactoferrin.1st Lactoferrin Forum, October 2004, Tokyo, Japan.
Other 156 presentations in domestic meetings
67
Original Articles
1.
Akiyama N, Shimma N, Takashiro Y, Hatori Y, Hirabayashi T, Horie S, Saito T and Murayama T (2005) Decrease in
cytosolic phospholipase A2alpha mRNA levels by reactive oxygen species via MAP kinase pathways in PC12 cells:
effects of dopaminergic neurotoxins. Cell Signal 17: 597-604.
2.
Araseki M, Kobayashi H, Hosokawa M and Miyashita K (2005) Lipid peroxidation of a human hepatoma cell line
(HepG2) after incorporation of linoleic acid, arachidonic acid, and docosahexaenoic acid. Biosci Biotechnol Biochem
69: 483-490.
3.
Azuma N, Kunihiro Y, Sasaki J, Nozawa Y, Mihara E, Mihara Y, Yasunaga T and Abe S (2005) Genetic variation of
Hair Crab (Erimacrus isenbeckii) inferred from mitochondrial DNA sequence analysis. Fish Genet Breed Sci, in press.
4.
Beppu F, Hosokawa M, Tanaka L and Miyashita K (2005) Potent inhibitory effect of trans9, trans11 isomer of
conjugated linoleic acid on the growth of human colon cancer cells. Mol Cell Biochem, in press.
5.
Chiba H, Ijiri S, Iwata M, Nakamura M, Adachi S and Yamauchi K (2005) Changes in serum steroid hormones during
ovarian development in the captive common Japanese conger Conger myriaster (Brevoort). Aquaculture Sci 53:
189-198.
6.
Doi T, Satoh A, Tanaka H, Inoue A, Yumoto F, Tanokura M, Ohtsuki I, Nishita K and Ojima T (2005) Functional
importance of Ca2+-deficient N-terminal lobe of molluscan troponin C in troponin regulation. Arch Biochem Biophys
436: 83-90.
7.
Edpalina RR, Sato S, Urawa S, Brykov V, Jin D-H, Urano A and Abe S (2005) Comparative population genetics of
chum and masu salmon using mitochondrial DNA sequence variation. NPAFC Tech Rep 6: 107-108.
8.
Fujita S, Iseki M, Yoshikawa S, Makino Y, Watanabe M, Motomura T, Kawai H and Murakami A (2005) Identification
and characerization of a fluorescent flagellar protein from the brown alga Scytosiphon lomentaria (Scytosiphonales,
Phaeophyceae): A flavoprotein homologous to old yellow enzyme. Eur J Phycol 40: 159-167.
9.
Fujita T, Fukada H, Shimizu M, Hiramatsu N and Hara A (2005) Annual changes in serum levels of two choriogenins
and vitellogenin in masu salmon, Oncorhynchus masou. Comp Biochem Physiol 141B: 211-217.
10.
Fujiwara Y, Fukada H, Shimizu M and Hara A (2005) Purification of two lipovitellins and development of
immunoassay for two forms of their precursors (vitellogenins) in medaka (Oryzias latipes). Gen Comp Endocrinol 143:
267-277.
11.
Fukada H, Ozaki Y, Pierce AL, Adachi S, Yamauchi K, Hara A, Swanson P and Dickhoff WW. (2005) Identification of
the salmon somatolactin receptor, a new member of the cytokine receptor family. Endocrinology 146: 2354-2361.
12.
Hakamata W, Muroi M, Kadokura K, Nishio T, Oku T, Kimura A, Chiba S and Takatsuki A (2005) Aglycon specificity
profiling of α-glucosidases using synthetic probes. Bioorg Med Chem Lett 15: 1489-1492.
13.
Hasegawa O, Ishibashi Y and Abe S (2005) Polymorphic microsatellite DNA markers for the rhinoceros auklet
(Cerorhinca monocerata). Mol Ecol Notes 5: 637-638.
14.
Horie S, Tsurumaki Y, Someya A, Hirabayashi T, Saito T, Okuma Y, Nomura Y and Murayama T (2005) Involvement of
cyclooxygenase-dependent pathway in contraction of isolated ileum by urotensin II. Peptides 26: 423-429.
15.
Hufana-Duran D, Duran PG, Kanai Y, Takahashi Y and Cruz LC (2005) Effect of density-dradiant sperm separation
technique on in vitro fertilization of water buffalo semen with low post-thaw motilities. Philippine Agricultural Scientist
88: 257-267.
16.
Ikadai H, Tanaka T, Shibahara N, Tanaka H, Matsuu A, Kudo N, Shimazaki K, Igarashi I and Oyamada I (2005)
Inhibitory effect of lactoferrin on in vitro growth of Babesia caballi. Am J Trop Med Hyg 73: 710-712.
17.
Ishida K, Imai H, Ogasawara K, Hagiwara K, Todo S, Furukawa H, Fujita H, Sakurai T and Tamashiro H (2005)
Cost-utility of living donor liver transplantation in a single Japanese center. Hepato-Gastroenterol, in press.
18.
Ishigamori H, Hosokawa M, Kohno H, Tanaka T, Miyashita K and Takahashi K (2005) Docosahexaenoic
acid-containing phosphatidylethanolamine enhances HL-60 cell differentiation by regulation of c-jun and c-myc
expression. Mol Cell Biochem 275: 127-133.
19.
Oshima K, Morishima K, Yamaha E and Arai K (2005) Reproductive capacity of triploid loaches obtained from
68
Hokkaido island, Japan. Ichthyol Res 52: 1-8.
20.
Kang HK, Seo MY, Seo ES, Kim D, Chung SY, Kimura A, Day DF and Robyt JF (2005) Cloning and expression of
levansucrase from Leuconostoc mesenteroides B-512FMC in Escherichia coli. Biochim Biophys Acta 1727: 5-15.
21.
Kasai H, Muto Y and Yoshimizu M (2005) Virucidal effects of ultraviolet, heat treatment and disinfectants against koi
herpesvirus (KHV). Fish Pathol 40: 137-138.
22.
Kato Y, Kogame K, Nagasato C and Motomura T (2005) Inheritance of mitochondrial and chloroplast genomes in the
isogamous brown alga Scytosiphon lomentaria (Phaeophyceae). Phycol Res, in press.
23.
Khan MMR, Arai K, Kuroda K, Umino T and Nakagawa H (2005) Genetic variation of Japanese loach inferred from
restriction fragment length polymorphism analysis of mitochondorial DNA. Affrican J Biotechnol 4: 318-325.
24.
Kim W-S, Rahman MM, Kumura H and Shimazaki K (2005) Comparison of growth promoting effects on
Bifidobacterium spp. by bovine lactoferrin hydrolysates. Bioscience Microflora 24: 119-123.
25.
Kim YM, Okuyama M, Mori H, Nakai H, Saburi W, Chiba S and Kimura A (2005) Enzymatic synthesis of alkyl
α-2-deoxyglucosides by alkyl alcohol resistant α-glucosidase from Aspergillus niger. Tetrahedron: Asymmetry 16:
403-409.
26.
Kitade Y, Nakamura M, Endo H, Fukuda S, Kuwano K and Saga N (2005) Characterization of a cDNA encoding a
homologue of actin-related protein 4 from a marine red alga, Porphyra yezoensis. Fish Sci, in press.
27.
Kitamura M, Ose T, Okuyama M, Watanabe H, Yao M, Mori H, Kimura A and Tanaka I (2005) Crystallization and
preliminary X-ray analysis of α-xylosidase from Escherichia coli. Acta Cryst F 61: 178-179.
28.
Kobayashi K, Wani JA, Kasai H, Nishizawa T and Yoshimizu M (2005) Virus surveillance of wild marine fishes
collected in coastal area of Hokkaido, Japan. Fish Pathol, 40: 29-31.
29.
Kobayashi M, Saitoh H, Yamaguchi M, Saito T, Fujita H, Suno M and Matsubara K (2005) Aungst BJ: Relationship
between loperamide-induced sedative effect and digoxin pharmacokinetics in healthy Japanese subjects. Pharmaceut
Res 22: 413-418.
30.
Kusuda S, Koide N, Kawamula H, Teranishi T, Nakajima J, Yamaha E, Arai K and Ohta H (2005) Cryoprreservation
diluents for spermatozoa of Sakhalin taimen, Hucho perryi. Fish Sci 71: 293-298.
31.
Li HM, Niki T, Taira T, Iguchi-Ariga SMM and Ariga H (2005) Association of DJ-1 with chaperones and enhanced
association and colocalization with mitochondrial Hsp70 by oxidative stress. Free Radic Res 39: 1091-1099.
32.
Maeda H, Hosokawa M, Sashima T, Funayama K and Miyashita K (2005) Fucoxanthin from edible seaweed, Undaria
pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem Biophys Res Comm
332: 392-397.
33.
Mahmoud BSM, Kawai Y, Yamazaki K, Miyashita K and Suzuki T (2005) A new technology of fish preservation by
combined treatment with electrolyzed NaCl solutions and essential oil compounds. Food Chem, in press.
34.
Mahmoud BSM, Yamazaki K, Miyashita K, Il-Shik S, Dong-Suk C and Suzuki T (2005) Preservative effect of
combined treatment with electrolyzed NaCl solutions and essential oil compounds on carp fillets during conventional
air-drying. Int J Food Microbiol, in press.
35.
Maita H, Kitaura H, Ariga H and Iguchi-Ariga SMM (2005) CIR, a corepressor of CBF1, binds to PAP-1 and effects
alternative splicing. Exp Cell Res 303: 375-387.
36.
Maita H, Kitaura H, Ariga H, and Iguchi-Ariga SMM (2005) Association of PAP-1 and Prp3p, the products of causative
genes of dominant retinitis pigmentosa, in the tri-snRNP complex. Exp Cell Res 302: 61-68.
37.
Matsubara H, Kazeto Y, Ijiri S, Hirai T, Adachi S and Yamauchi K (2005) Serum steroid profiles in artificially maturing
female Japanese eel, Anguilla japonica. Aquaculture 243: 393-402.
38.
Matsuda E, Ishizaki R, Taira T, Iguchi-Ariga SMM and Ariga H (2005) Structure and characterization of AAT-1
isoforms. Biol Pharm Bull 28: 898-901.
39.
Moriya S, Sato S, Azumaya T, Suzuki O, Urano A and Abe S (2005) Genetic Stock identification of chum salmon
(Oncorhynchus keta) in the Bering Sea using DNA microarray NPAFC Tech Rep 6: 86-87.
40.
Nabemoto M, Ohsawa K, Nakamura H, Hirabayashi T, Saito T, Okuma Y, Nomura Y, Murayama T (2005) Reversible
activation of secretory -hospholipase A2 by sulfhydryl reagents. Arch Biochem Biophys 436: 145-153.
41.
Nagae M, Shiroyama K, Inoue M, Hara A, Takano Y, Kohra S, Ishibashi Y, Tominaga N, Yoshihara S and Arizono K
69
(2005) Estrogenic potency of a bisphenol A metabolite on vitellogenin synthesis in medaka, Oryzias ratipes. J Health
Sci 51: 93-95.
42.
Nagasato C (2005) Behavior and function of paternally inherited centrioles in brown algal zygotes. J Plant Res, in
press.
43.
Nakai H, Okuyama M, Kim Y-M, Saburi W, Wongchawalit J, Mori H, Chiba S and Kimura A (2005) Molecular analysis
of α-glucosidase belonging to GH-family 31. Biologia 60: 131-135.
44.
Nakamura A, Watanabe K, Ojima T, Ahn D-H and Saeki H (2005) Effect of maillard reaction on allergenicity of scallop
tropomyosin. Agric Food Chem 53: 7559-7564.
45.
Nishimura K, Suzuki T, Momchilova S, Miyashita K, Katsura E and Itabashi Y (2005) Analysis of conjugated linoleic
acids as 9-anthrylmethyl esters by reversed-phase high-performance liquid chromatography with fluorescence detection.
J Chromatograph Sci 43: 494-499.
46.
Nishinaga H, Takahashi-Niki K, Taira T, Andreadis A, Iguchi-Ariga SMM and Ariga H (2005) Expression profiles of
genes in DJ-1-knockdown and L166P DJ-1 mutant cells. Neuroscience Lett 390: 54-59.
47.
Nishizawa T, Kinoshita S and Yoshimizu M (2005) An approach for genogrouping of Japanese isolates of
aquabirnavirus in a new genogroup, VII, based on the VP2/NS junction region. J Gen Virol 86: 1973-1978.
48.
Nomura J, Matsumoto K, Iguchi-Ariga SMM and Ariga H (2005) Positive regulation of Fas gene expression by MSSP
and abrogation of Fas-mediated apoptosis induction in MSSP-deficient mice. Exp Cell Res 305: 324-332.
49.
Nomura J, Matsumoto K, Iguchi-Ariga SMM and Ariga H. (2005) Mitochondria-independent induction of
Fas-mediated apoptosis by MSSP. Oncology Rep 14: 1305-1309.
50.
Oda A, Miki H, Wada I, Yamaguchi H, Yamazaki D, Suetsugu S, Nakajima M, Nakayama A, Okawa K, Miyazaki H,
Matsuno K, Ochs HD, Machesky LM, Fujita H and Takenawa T (2005) WAVE/Scars in platelets. Blood 105:
3141-3148.
51.
Okuda K, Oda A, Sato Y, Nakayama A, Fujita H, Sonoda Y and Griffin JD (2005) Signal transduction and cellular
functions of the TEL/ARG oncoprotein. Leukemia 19: 603-10.
52.
Okuyama M, Tanimoto Y, Ito T, Anzai A, Mori H, Matsui H, Chiba S and Kimura A (2005) Purification and
characterization of hyper-glycosylated α-glucosidase from Schizosaccharomyces pombe culture medium. Enzyme
Microb Technol 35: 472-480.
53.
Omoto N, Maebayashi M, Adachi S, Arai K and Yamauchi K (2005) Sex ratios of triploids and gynogenetic diploids
induced in the hybrid sturgeon, the bester (Huso huso female X Acipenser ruthenus male). Aquaculture 245: 39-47.
54.
Omoto N, Maebayashi M, Adachi S, Arai K and Yamauchi K (2005) The influence of oocyte maturational stage on
hatching and triploidy rates in hybrid (bester) sturgeon. Aquaculture 245: 287-294.
55.
Ono S, Hosokawa M, Miyashita K and Takahashi K (2005) Inhibition properties of dipeptides from salmon muscle
hydrolysate on angiotensin I-converting enzyme. Int J Food Sci Tech 40: 1-4.
56.
Ooe H, Taira T, Iguchi-Ariga SMM and Ariga H (2005) Induction of reactive oxygen species by bisphenol A and
abrogation of bisphenol A-induced cell injury by DJ-1. Toxicol Sci 88: 114-126.
57.
Oshima K, Morishima K, Yamaha E and Arai K (2005) Reproductive capacity of triploid loaches obtained from
Hokkaido Island, Japan. Ichthyological Research, 52:1-8 (2005)
58.
Otani S, Kitauchi T, Saito T, Sakao S, Maegawa S, Inoue K, Arai K and Yamaha E (2005) The formation of primoridial
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Sakaguchi M, Suzuki T, Sasamoto Y, Takahashi Y, Nishiura A and Aoki M (2005) Effects of first breeding age on the
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61.
Sato F, Okuyama M, Nakai H, Mori H, Kimura A and Chiba S (2005) Glucoamylase originating from Schwanniomyces
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62.
Sato S, Hori Y, Yamate J, Saito T, Kurasaki M and Hatai A (2005) Protective effect of dietary azuki bean (Vigna
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63.
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64.
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Sekito A, Taira T, Niki T, Iguchi-Ariga SMM and Ariga H (2005) Stimulation of transforming activity of DJ-1 by
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68.
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69.
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78.
Yasui Y, Hosokawa M, Kohno H, Tanaka T and Miyashita K (2005) Comparison of growth inhibition and apoptosis
induction by troglitazone and 9cis, 11trans, 13trans-conjugated linolenic acid on different colon cancer cell lines.
Chemotherapy, in press.
79.
Yasui Y, Hosokawa M, Sahara T, Suzuki R, Ohgiya S, Kohno H, Tanaka T and Miyashita K (2005) Bitter gourd seed
fatty acid rich in 9c,11t,13t-conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPARγ
in human colon cancer Caco-2 cells. Prostag Leukotr Ess 73: 113-119.
80.
Yoshikubo H, Suzuki N, Takemura K, Hoso M, Yashima S, Iwamuro S, Takagi Y, Tabata MJ and Hattori A (2005)
Osteoblastic activity and estrogenic response in the regenerating scale of goldfish, a good model of osteogenesis. Life
Sci 76: 2699-2709.
81.
Yoshimizu M, Yoshinaka T, Hatori S and Kasai H (2005) Survivability of fish pathogenic viruses in environmental
water, and inactivation of fish viruses. Bull Fish Res Agen Supplement No.2: 47-54.
82.
Yumoto F, Lu Q-W, Morimoto S, Tanaka H, Kouno N, Nagata K, Ojima T, Takahashi-Yanaga F, Miwa Y, Sasaguri T,
Nishita K, Tanokura M and Ohtsuki I (2005) Drastic Ca2+ sensitization of myofilament associated with a small
structural change in troponin I in inherited restrictive cardiomyopathy. Biochem Biophys Res Commun 338:
1519-1526.
71
Other 8 papers published in domestic Japanese journals.
Reviews and Books
1.
Hiramatsu N, Cheek AO, Sullivan CV, Matsubara T and Hara A (2005) Vitellogenesis and endocrine disruption. In
Biochemistry and Molecular Biology of Fishes, Vol 6 (Mommsen TP and Moon TW eds), Elsevier B.V., pp. 431-471.
2.
Hiramatsu N, Matsubara T, Fujita T, Sullivan CV and Hara A (2005) Multiple piscine vitellogenins:
Biomarkers of fish exposure to estrogenic endocrine disruptors in aquatic environments. Mar Biol. in
press
3.
Kawai H, Motomura T and Okuda K (2005) Isolation and purification techniques for macroalgae. In Algal Culturing
Techniques (Andersen RA ed), Elsevier Academic Press, pp. 133-144.
Other 18 review papers published in Japanese books and domestic journals.
Others
1.
Nakai H, Iizuka T, Fukukawa T, Nishioka K, Tanizawa S, Okuyama M, Mori H, Chiba S and Kimura A (2005)
Screening of enzymes having excellent functions from the digestive caecum of scallop. The 2nd International
Symposium, “Marine Resources as Functional and Therapeutic Agents”, 21st Century COE Program “Marine
Bio-Manipulation Frontier for Food Production, Toward Advanced and Safe Use of Aquatic Organisms”, May 2005,
Sapporo, pp. 37.
2.
Nakai H, Tanizawa S, Ito S, Ito T, Matsubara K, Okuyama M, Mori H, Chiba S, Sano Y and Kimura A (2005)
Functional analysis in glycoside hydrolase family 31 enzymes from rice seeds (Oryza sativa L. var Nipponbare), 6th
Carbohydrate Bioengineering Meeting, April 2005, Barcelona, Spain.
3.
Saburi W, Kim YM, Okuyama M Mori H, Yu S and Kimura A (2005) Enzymatic Hydration of 1,5-Anhydrofructose by
α-Glucosidase. The 1st International Symposium, “Potential and Perspective of Marine Bio-Manipulation”, 21st
Century COE Program “Marine Bio-Manipulation Frontier for Food Production, Toward Advanced and Safe Use of
Aquatic Organisms”, February 2005, Sapporo, Japan.
4.
Saburi W, Kim YM, Okuyama M, Mori H, Yu S, Chiba S and Kimura A (2005) Reaction of α-glucosidase on
1,5-anhydrofructose. 6th Carbohydrate Bioengineering Meeting, April 2005, Barcelona, Spain.
5.
Saburi W, Kim YM, Okuyama M, Mori H, Yu S, Chiba S and Kimura A (2005) Reaction of α-glucosidase on
1,5-anhydrofructose. The 4th International Symposium, “Reproductive, Genetic and Disease Management in
Aquaculture and Ocean Ranching”, 21st Century COE Program “Marine Bio-Manipulation Frontier for Food
Production, Toward Advanced and Safe Use of Aquatic Organisms”, October 2005, Hakodate.
6.
Sakaki K, Matsubara H, Hirai T, Hara A and Nakamura M (2005) Effects of benzophenone on testicular differentiation
in carp Cyprinus carpio. International Symposium on the Environmental Risk of Endocrine Disrupter – Fruits of
Research and Future Perspectives –, January 22-23, Kyoto, Japan, Abstract, P-28.
7.
Ura K, Miyake M, Koga Y, Todo T, Hara A and Takagi Y (2005) Immunochemical and biochemical studies on egg yolk
protein in sea urchin, Strongylocentrotus intermedius. 2nd Japan-Korea, Korea-Japan Joint Meeting on Reproductive
Biology of Aquatic Animals, November 2005, Kumamoto, Japan, pp. 10.
8.
Wongchawalit J, Nakai H, Kim YM, Yamamoto T, Okuyama M, Mori H, Svasti J, Chiba S and Kimura A (2005)
Molecular analysis of α-glucosidase isozymes from Japanese honeybee (Apis cerana). 30th FEBS Congress and 9th
IUBMB Conference, July 2005, Budapest, Hungary.
9.
Yoon M, Sato S, Seeb JE, Wilmot RL, Urawa S, Urano A and Abe S (2005) Genetic variation among chum salmon
populations in the Pacific Rim inferred from the mitochondrial and microsatellite DNA analyses. NPAFC Document
No.898: 1-20.
72
10.
fishing through processing to distribution. In Proceedings of the 4th FiSCUP Japan-Korea Joint Seminar on Fisheries
Sciences, December 15-16, 2004, Sapporo, Japan, pp.24-28.
11.
Yoshitani K, Inoue M, Fukada H, Shimizu M, Fujita T and Hara A (2005) Two distinct choriogenins in medaka:
development of a specific chemiluminescent immunoassay and verification of their estrogen-inducibility. International
Symposium on The Environmental Risk of Endocrine Disrupter – Fruits of Research and Future Perspectives –,
January 22-23, Kyoto, Japan, Abstract, P-4.
Other 24 reports published in Japanese.
Patents
1.
2005-150490 by Miyashita et al
2.
2005-1266467 by Miyashita et al.
3.
2005-313929 by Sashima et al.
4.
1.
Amano H, Fujita T, Hiramatsu N, Yeo I-K, Kagawa H, Sullivan CV and Hara A (2005) Detection of three vitellogenins
in serum of grey mullet. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”,
21st COE Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo, Japan.
2.
Azuma N and Abe S (2005) Development and application of molecular genetic markers in marine animals including
crustacea. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st COE
Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo, Japan.
3.
Edpalina RR, Urawa S, Urano A and Abe S （2005） Molecular population genetics of masu salmon based on
mitochondrial DNA sequence analysis.
The 1st International Symposium, “Potential and Perspective of
st
Marine-Bio-manipulation”, 21 COE Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo,
Japan.
4.
Fujita T, Fukada H and Hara A (2005) cDNA cloning and expression of zona rpellucida protein B and C in masu
salmon (Oncorhynchus masou).
st
Japan.
5.
Inoue A, Yamamoto S and Ojima T (2005) Bacterial expression of endo-type alginate lyase from abalone. The 1st
International Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st COE Program “Marine
Bio-Manipulation Frontier for Food Production”, Sapporo, Japan.
6.
Inoue M, Yoshitani K, Fukada H, Shimizu M, Fujino H, Fujita T and Hara A (2005) Changes of dual vitellogenins in
serum of medaka (Oryzias latipes) exposed to estrogen under different conditions. The 1st International Symposium,
“Potential and Perspective of Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for
Food Production”, Sapporo, Japan.
7.
Ito D and Abe S (2005) Abnormal embryogenesis and chromosome abnormalities in the inviable hybrid between
Japanese huchen and rainbow trout.
st
Japan.
8.
Kobayashi Y, Takamura T, Shimono I, Ura K and Takagi Y (2005) Discrimination of scallop populations using minor
and trace elemental fingerprints in the shell. Satellite Symposium of The 1st International Symposium, “Potential and
Perspective of Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”，
Eniwa, Japan．
9.
Miyake M, Yuba Y, Koga Y, Ura K and Takagi Y (2005) cDNA cloning of the major yolk protein in sea urchin,
73
Strongylocentrotus intermedius. Satellite Symposium of The 1st International Symposium, “Potential and Perspective
of Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”，Eniwa,
Japan
10.
Kazeto Y, Todo T, Adachi S, Yamauchi K and Trant JM (2005) Molecular biology of channel catfish brain cytochrome
P450aromatase (CYP19A2): cloning, preovulatory induction of gene expression, hormonal gene regulation and analysis
of its promoter region. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”,
11.
Kai T, Saga N, Yasui H and Mizuta H (2005) A hypothetical control model on sorus formation by auxin in Laminaria
japonica (Phaeophyceae). The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”,
12.
Katoh M, Tanaka H, Suzuki H, Inoue A and Ojima T (2005) Biochemical significances of N-terminal lobe of akazara
scallop TnC in troponin regulation.
st
Japan.
13.
Nakamura T, Murayama S, Fujita T, Ohkubo N, Matsubara T and Hara A (2005) Androgenic and estrogenic effects of
the synthetic androgen 17a-methyltestosterone in the immature three-spined stickleback (Gasterosteus aculeatus). 1st
International Symposium of “Potential and porspective of Marine Bio-Manipulation”, Sapporo, Japan.
14.
Nakano D, Ura K, Shimizu M and Takagi Y (2005) Histological studies of the digestive system of Strongylocentrotus
intermedius. Satellite Symposium of The 1st International Symposium, “Potential and Perspective of
Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”，Eniwa, Japan．
15.
Nishida Y, Suzuki K and Ojima T (2005) Characterization and cDNA cloning of a cellulase from sea urchin
Strongylocentrotus nudus. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”,
16.
Oda A, Fujita H (2005) CrkL/Crk directs DOCK5 to peripheral focal adhesions. Gordon Research Conference: Cell
Biology Of Megakaryocytes & Platelets, Buellton, USA.
17.
Ogawa N, Ohira Y, Shimizu M, Ura K and Takagi Y (2005) Regenerating scale of goldfish: a new model for bone
calcification and resorption. Satellite Symposium of The 1st International Symposium, “Potential and Perspective of
Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”，Eniwa, Japan．
18.
Saburi W, Kim Y-M, Okuyama M, Mori H, Yu S and Kimura A (2005) Enzymatic Hydration of 1,5-Anhydrofructose by
a-Glucosidase. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st COE
19.
Sasaki K, Matsubara H, Hirai T, Hara A and Nakamura M (2005) Effects of benzophenone on testicular differentiation
in carp Cyprinus carpio. International Symposium on The Environmental Risk of Endocrine Disrupter – Fruits of
Research and Future Perspectives, Kyoto, Japan.
20.
Senaha A, Matsubara H, Todo T, Adachi S and Yamauchi K (2005) Effects of androgen on the expression of lipidation
related genes in female Japanese eel (Anguilla japonica). The 1st International Symposium, “Potential and Perspective
of Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo,
Japan.
21.
Shimizu K, Senaha A, Ishigami H, Shin D-H, Todo T, Adachi S and Yamauchi K (2005) Artificial maturation of female
Japanese eels, Anguilla japonica, pretreated with androgen. The 1st International Symposium, “Potential and
Perspective of Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”,
Sapporo, Japan.
22.
Shin D-H, Ishigami H, Haraguchi I, Yamashita M, Kobayashi T, Kobayashi H, Nagahama Y, Todo T, Adachi S and
Yamauchi K (2005) Biochemical and histological studies on egg quality in Japanese eel (Anguilla japonica) and
goldfish (Carassius auratus).
st
Japan.
23.
Suzuki H, Suzuki K, Inoue A and Ojima T (2005) Characterization and cDNA cloning of a subtilase from sea urchin
Strongylocentrotus nudus. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”,
24.
Suzuki H, Suzuki K, Inoue A and Ojima T (2005) Isolation and cDNA cloning of exo-type mannuronate lyases from the
74
pacific abalone Haliotis discus hannai.
st
Japan.
25.
Suzuki K and Ojima T (2005) Biochemical properties of abalone cellulases. The 1st International Symposium,
26.
Takahashi A, Ijiri S, Todo T, Adachi S and Yamauchi K (2005) Changes in mRNA levels of two types of P450
aromatase during sex determination period in the goldfish (Carassius auratus). The 1st International Symposium,
27.
Takahashi T, Mochizuki M, Fujita T, Fukada H, Yusa S, Takagi S and Hara A (2005) Changes in serum vitellogenin and
choriogenins levels in chum salmon (Oncorhynchus keta) during the migration. The 1st International Symposium,
28.
Takei N, Kazeto Y, Ijiri S, Todo T, Adachi S and Yamauchi K (2005) Changes in expression of steroidogenic enzymes
during gonadal development of Japanese eel, Anguilla japonica. The 1st International Symposium, “Potential and
Perspective of Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”,
Sapporo, Japan.
29.
Wada T, Amano H, Fujita T and Hara A (2005) Serum levels of main vitellogenin (Vg1) in red lip mullet (Chelon
haematocheilus). The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st
COE Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo, Japan.
30.
Yamaha, E (2005) Bio-manipulation of fish embryos toward surrogate production in aquaculture. The 1st International
Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation
Frontier for Food Production”, Sapporo, Japan.
31.
Yamamoto S, Suzuki H, Suzuki K, Inoue A and Ojima T (2005) Production of oligoalginates from Laminaria by the
digestion with abalone alginate lyase.
st
Japan.
32.
Yasui Y, Hosokawa M, Kohno H, Tanaka T and Miyashita K (2005) Chemopreventive effect of conjugated trienoic acid
on colon cancer. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st COE
33.
Yoon M, Sato S, Urawa S, Urano S and Abe S (2005) Motichondrial DNAanalysis of genetic population structure of
chum salmon (Oncorhynchus keta) in the Pacific Rim. The 1st International Symposium, “Potential and Perspective of
Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo,
Japan.
34.
Yoshida Y, Ura K, Simizu M, Sakurai Y and Takagi Y (2005) Development of the statocyst and statolith in the Japanese
common squid, Tadarodes pacificus: ultrastructural observations. Satellite Symposium of The 1st International
Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st COE Program “Marine Bio-Manipulation
Frontier for Food Production”，Eniwa, Japan.
35.
Yoshitani K, Inoue M, Fujita T and Hara A (2005) Development of a chemiluminescent immunoassay for two
choriogenins in medaka (Oryzias latipes). The 1st International Symposium, “Potential and Perspective of
Japan.
36.
Yoshitani K, Inoue M, Fukada H, Shimizu M, Fujita T and Hara A (2005) Two distinct choriogenins in medaka:
development of a specific chemiluminescent immunoassay and verification of their estrogen-inducibility.
International Symposium on The Environmental Risk of Endocrine Disrupter – Fruits of Research and Future
Perspectives, Kyoto, Japan.
37.
Abe S (2005) Mitochondrial DNA SNPs detected in the Pacific Rim populations of chum salmon. SNP Workshop:
Developing Cooperative Databases for Pacific Salmon, Anchorage, USA. （Invited）
38.
Abe S, Moriya S, Sato S, Yoon M, Azumaya T, Urawa S and Urano A (2005) DNA microarray analysis on board ships
for genetic stock identification of chum salmon in the Bering Sea and adjacent waters. Science Bridging Five
75
Nations: The Bering-Aleutian Salmon International Survey, Symposium at the 135th Annual Meeting of American
Fisheries Society, Anchorage, USA. （Invited）
39.
Abe T, Sago Y, Shin D-H, Miyashita T, Miura M, Todo T, Adachi S and Yamauchi K (2005) Storage of unfertilized eggs
of fish in artificial media. The 4th International Symposium “Reproductive, Genetic and Disease Management in
Aquaculture and Ocean Ranching”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”,
Hakodate, Japan.
40.
Amano H, Fujita T, Hiramatsu N, Sawaguchi S, Matsubara T, Kagawa H, Sullivan CV and Hara A (2005) Multiple
lipovitellins in grey mullet: N-terminal amino acid sequencing and mapping of their constituent polypeptides. The 4th
International Symposium “Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching”, 21st
COE Program “Marine Bio-Manipulation Frontier for Food Production”, Hakodate, Japan. （Best Poster Award）
41.
Aoki J, Hatsuyama A, Imamura Y, Nagae M, Takao Y, Hara A, Lee YD, Yeo I-K and Soyano K (2005) Concentration of
the vitellogenin in serum of wild grey mullet at Korea and Japan. 2nd Japan-Korea, Korea-Japan Joint Meeting on
Reproductive Biology of Aquatic Animals, Aso, Japan.
42.
Arai K (2005) Research Core of Genetics and Developmental Biology for Innovative Aquaculture System. The 3rd
International Symposium, “Industrial Academia-Governmental Collaboration for the Establishment of Marine Food
Production in China and Japan”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production” as
Commemorative Program for Agreement of Academic Exchange between Shanghai Fisheries University and Hokkaido
University, Shanghai, China.
43.
Arai K (2005) The initiative in applied life science for aquaculture and marine bio-industry. The 3rd International
Symposium, “Industrial Academia-Governmental Collaboration for the Establishment of Marine Food Production in
China and Japan”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production” as Commemorative
Program for Agreement of Academic Exchange between Shanghai Fisheries University and Hokkaido University,
Shanghai, China.
44.
Aruas-Rodriguez L, Morishima K and Arai K (2005) New microsatellite markers in Misgurnus anguillicaudatus for
population identification in Hokkaido.
st
Japan.
45.
Bando E, Shiokawa Y, Morishima K and Arai K (2005) Clonal lineage of the loach Misgurnus anguillicaudatus
(Teleostei: Cobitidae) inferred from DNA markers. The 1st International Symposium, “Potential and Perspective of
Japan.
46.
Bubenshchikova E, Kaftanovskaya E, Arai K, Fujimoto T, Kinoshita M, Ozato K and Wakamatsu Y (2005) Diploid
induced-recipient eggs lead reconstructed embryos by nuclear transfer of adult cells to adult individuals in medaka
(Oryzias latipes). 15th International Society of Developmental Biologists Congress, Sydney, Australia.
47.
Edpalina RR, Sato S, Urawa S, Brykov V, Urano A and Abe S (2005) Genetic variation and population structure of
masu salmon based on mitochondrial DNA sequence analysis. Annual Meeting of JSFS, 2005, Tokyo, Japan．
48.
Endo H (2005) Porphyra yezoensis as a marine model plant. 7th International Marine Biotechnology Conference, St.
John's, Canada.
49.
Fujimoto T, Saito T, Sakao S, Yamaha E and Arai K (2005) Developmental staging and germ cell lineage in loach,
Misgurnus anguillicaudatus.
st
Japan.
50.
Fujita T and Hara A (2005) Expression of recombinant choriogenin, egg chorion precursor.
Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals, Aso, Japan.
51.
Fujita T, Fukada H and Hara A (2005) Expression of recombinant choriogenin, egg chorion precursor, in masu salmon
(Oncorhynchus masou). The 4th International Symposium “Reproductive, Genetic and Disease Management in
Hakodate, Japan.
52.
Fujita T, Inoue M, Yoshitani K, Takahashi T, Mochizuki M, Amano H and Hara A (2005) Monitoring system for
environmental estrogens using fish serum proteins – current research for multiple marker proteins-. The 2nd
International Symposium, “Marine resources as functional and therapeutic agents”, 21st COE Program “Marine
76
2nd Japan-Korea,
53.
Fukada H, Hara A, Swanson P and Dickhoff WW (2005) Differential effects of feeding and fasting on growth hormone
receptor (GHR) and somatolactin receptor (SLR) in liver and muscle of coho salmon (Oncorhynchus kisutch). 15th
International Congress of Comparative Endocrinology, Boston, USA.
54.
Fukui D, Bando G, Nakata S, Nagano M, Sakamoto H, Sirouzu A, Shimura R, Ise S, Kusuda T, Putranto HD, Doi M,
Kosuge M and Takahashi Y (2005) Semen collection via electroejaculation and artificial intravaginal insemination with
fresh semen in the amur tiger (Panthera tigris altaica). Asian Meeting for Zoo and Wildlife Medicine, Bangkok,
Thailand.
55.
Goto-Kazeto R, Saito T, Kayaba T, Todo T, Arai K and Yamaha E (2005) Isolation and characterization of Vasa and
Nanos1 in barfin flounder. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”,
56.
Hara A (2005) Estrogenic impacts in coastal aquatic environments: Surveys on serum vitellogenin levels in the grey
mullet and the red lip mullet. The satellite meeting of POME, Tokyo, Japan.
57.
Hara A (2005) Multiple biomarkers for environmental estrogen. The 3rd International Symposium, “Industrial
Academia-Governmental Collaboration for the Establishment of Marine Food Production in China and Japan”, 21st
COE Program “Marine Bio-Manipulation Frontier for Food Production” as Commemorative Program for Agreement of
Academic Exchange between Shanghai Fisheries University and Hokkaido University, Shanghai, China.
58.
Hosokawa M, Maeda H and Miyashita K (2005) Anti-obesity effects of seaweed lipids containing fucoxanthin.
World Congress and Exhibition of the International Society for Fat Research, Prague Czech.
59.
Hosokawa M, Yamamoto Y, Kuwagaki Y and Miyashita K (2005) Enzymatic preparation of conjugated fatty
acid-containing phospholipids and triacylglycerol. 96th AOCS Annual Meeting & Expo, Salt Lake City, USA.
60.
Hotta K, Watanabe T, Nakamura Y, Ijiri S, Kazeto Y, Todo T, Adachi S and Yamauchi K (2005) Effects of temperature
on oocyte maturation in Japanese whiting, Sillago japonica. The 4th International Symposium “Reproductive, Genetic
and Disease Management in Aquaculture and Ocean Ranching”, 21st COE Program “Marine Bio-Manipulation Frontier
for Food Production”, Hakodate, Japan.
61.
Ishigami H, Shin D-H, Haraguchi I, Unuma T, Kurokawa T, Nomura K, Matsubara H, Tanaka H, Todo T, Adachi S and
Yamauchi K (2005) Hatching rate and thyroid hormone levels in eggs from artificially matured Japanese eel. The 4th
International Symposium “Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching”, 21st
COE Program “Marine Bio-Manipulation Frontier for Food Production”, Hakodate, Japan.
62.
Ishiizaki R, Shin H-W, Iguchi-Ariga SMM, Ariga H and Nakayama K (2005) AMY-1 is localized on the trans-Golgi
network in association with brefeldin A-inhibited guanine nucleotide-exchange factor 2 (BIG2). 78th Meeting of
Japan Society of Biochemistry, Kobe, Japan.
63.
Kasai H and Yoshimizu M (2005) Elimination of Escherichia coli from oyster using electrolyzed seawater. The 2nd
International Symposium, “Marine resources as functional and therapeutic agents”, 21st COE Program “Marine
64.
Kasai H and Yoshimizu M (2005) Elimination of Escherichia coli from oyster using electrolyzed seawater. The 1st
International Oyster Symposium, Tokyo, Japan.
65.
Kasai H, Nakata Y and Yoshimizu M (2005) Elimination of norovirus (NV) from oyster -Survival of Feline Calicivirus
(FCV), a NV surrogate, in marine environments and under inactivating conditions-. The 1st International Oyster
Symposium, Tokyo, Japan.
66.
Kasai H, Shimizu T, Muto Y and Yoshimizu M (2005) Survivability of koi herpesvirus (KHV) in environmental water
as well as physical and chemical inactivation. The 12th International Conference on “Disease of Fish and Shellfish”,
Copenhagen, Denmark.
67.
Kasai H, Shimizu T, Muto Y and Yoshimizu M (2005) Survivability of koi herpesvirus (KHV) in environmental water
as well as physical and chemical inactivation. The 4th International Symposium “Reproductive, Genetic and Disease
Management in Aquaculture and Ocean Ranching”, 21st COE Program “Marine Bio-Manipulation Frontier for Food
Production”, Hakodate, Japan.
68.
Kawakami Y, Adachi S, Yamauchi K and Ohta H (2005) Characterization of thyroid hormone receptor β2 in the
Japanese eel, Anguilla Japonica. The 4th International Symposium “Reproductive, Genetic and Disease Management
in Aquaculture and Ocean Ranching”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”,
Hakodate, Japan.
77
26th
69.
Kayaba T, Sugimoto T, Matsuda T, Adachi S and Yamauchi K (2005) Environmental factors regulating spontaneous
spawning in cultured barfin flounder Verasper moseri. The 4th International Symposium “Reproductive, Genetic and
Disease Management in Aquaculture and Ocean Ranching”, 21st COE Program “Marine Bio-Manipulation Frontier for
Food Production”, Hakodate, Japan.
70.
Kazeto Y, Todo T, Adachi S and Yamauchi K (2005) Cloning and characterization of a cDNA encoding cholesterol
side-chain cleavage cytochrome P450: the tissue-distribution and changes in the transcript abundance in ovarian tissue
of Japanese eel, Anguilla japonica, during sexual maturation. The 2nd International Symposium, “Marine resources as
functional and therapeutic agents”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”,
Sapporo, Japan.
71.
Kazeto Y, Todo T, Adachi S, Yamauchi K and Trant JM (2005) Hormonal regulation of the expression of the genes
associated with reproductive physiology in the overy of channel catfish. The 4th International Symposium
“Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching”, 21st COE Program “Marine
Bio-Manipulation Frontier for Food Production”, Hakodate, Japan.
72.
Kim S-G, Morishima K, Sato N, Fujioka T, Saito S and Arai K (2005) Development of microsatellite DNA markers in
brown saole, Pleuronectes herzensteini. 6th International Symposium on Flatfish Ecology, Maizuru, Japan.
73.
Kim S-G, Morishima K, Satoh N, Fujioka T, Saito S and Arai K (2005) Isolation and characterization of microsatellite
DNA markers in brown sole, Pleuronectes herzensteini. The 2nd International Symposium, “Marine resources as
functional and therapeutic agents”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production,
Sapporo, Japan.
74.
Kim WS, Hori Y, Yoshinaka T, Kasai H, Nishizawa T and Yoshimizu M (2005) Evaluation of methods for
sero-epidemiology and surveillance of infectious hematopoietic necrosis (IHN). The 5th Joint Seminar between Korea
and Japan by KOSEF･JSPS Core Univ. Program on Fisheries Sciences-Zero Emission Fishery Industry: Present and
Future-, Kunsan, Korea.
75.
Kim, H-R, Kim Y-M, Lee G-H, Kang S-C, Miyashita K, Hou CT and Kim D-S (2005) Vegetable fatty acids
bio-conversion by Pseudomonas aeruginosa PR3 efficiently enhanced oxidation of fish oil. 96th AOCS Annual
Meeting & Expo, Salt Lake City, USA.
76.
Koba K, Akahoshi A, Tanaka K, Miyashita K, Hosokawa M, Kohno-Murase J, Imamura J and Sugano M (2005) Effects
of dietary plant seed oils containing conjugated linolenic acid on body fat mass and lipid metabolism in rodents. 96th
AOCS Annual Meeting & Expo, Salt Lake City, USA.
77.
Kobayashi Y, Takamura T, Shimono I, Ura K and Takagi Y (2005) Discrimination of scallop populations using minor
and trace elemental fingerprints in the shell. The 1st International Symposium, “Potential and Perspective of
Japan.
78.
Koide-Yoshida S, Taira T, Iguchi-Ariga SMM and Ariga H (2005) Functional analysis of DJ-1 in familial amyloidotic
polyneuropathy. The 4th International Symposium “Reproductive, Genetic and Disease Management in Aquaculture
and Ocean Ranching”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production” Hakodate, Japan.
79.
Kubota Y, Hamaoka M, Todo T, Ura K, Takagi Y, Adachi S and Yamauchi K (2005) Sea urchin vasa-related genes:
specific makers of the germline in Strongylocentroutus intermedius. The 4th International Symposium “Reproductive,
Genetic and Disease Management in Aquaculture and Ocean Ranching”, 21st COE Program “Marine Bio-Manipulation
Frontier for Food Production”, Hakodate, Japan.
80.
Kume G, Horiguchi T, Maruo N, Hara A, Shiraishi H, Shibata Y, Morita M and Shimizu M (2005) Determination of the
factors to cause the population decline of marbled sole Pleuronectes yokohamae in Tokyo Bay: Investigations on
possible adverse effects by environmental chemicals. 6th International Symposium on Flatfish Ecology, “Habitats in
relation to recruitment variability –Generation of future direction-“, Maizuru, Japan.
81.
Kusuda S, Teranishi T, Koide N, Yamaha E and Arai K (2005) Cryopreservation of chum salmon (Oncorhynchus keta)
blastomeres by a cryovial method:effects of cooled temperature and cooling rate on post-thaw survival rates. The 1st
82.
Li HM, Ariga H and Iguchi-Ariga SMM (2005) DJ-1, an oxidative stress-responsive gene, as a usuful monitor of
environmental materials in fish cells. The 4th International Symposium “ Reproductive, Genetic and Disease
Production” Hakodate, Japan.
78
83.
Li HM, Niki T, Taira T, Iguchi-Ariga SMM and Ariga H (2005) Association of DJ-1 with chaperones and enhanced
association and colocalization with mitochondrial Hsp70 by oxidative stress. 28th Annual Meeting of Japan Society of
Molecular Biology, Fukuoka..
84.
Maeda H, Hosokawa M and Miyashita K (2005) Fucoxanthin as a potential anti-obesity compound.
Annual Meeting & Expo, Salt Lake City, USA.
85.
Maeda H, Hosokawa M, Sashima N, Funayama K and Miyashita K (2005) Fucoxanthin from edible seaweed (Undaria
pinnatifida) shows antiobesity effect through UCP1 expression in white adipose tissues. The 14th International
Symposium on Carotenoids, Edinburgh, UK.
86.
Rahman MdM, Ito T, Kumura H and Shimazaki K (2005) Detection of lactoferrin-binding protein on bifidobacteria by
far-western blotting and fluorescence microscopy. Sapporo Meeting of the Japan Society for Bioscience,
Biotechnology and Agrochemistry , Sapporo, Japan.
87.
Rahman MdM, Kim W-S, Ito T, Kumura H and Shimazaki K, (2005) Detection of lactoferrin binding protein on
Bifidobacteria. 4th NIZO Dairy Conference, Papendal, The Netherlands.
88.
Rahman MdM, Kim W-S, Ito T, Kumura H and Shimazaki K (2005) Visualization of lactoferrin binding to
Bifidobacteria. 7th International Conference on Lactoferrin, Honolulu, USA.
89.
Rahman MdM, Kim W-S, Ito T, Kumura H and Shimazaki K, (2005) Behavior of bovine lactoferrin on the growth of
Bifidobacterium longum. Meeting of Hokkaido Chapter, Japan Society for Bioscience, Biotechnology and
Agrochemistry, Obihiro, Japan.
90.
Miyake M, Yuba Y, Koga Y, Ura K and Takagi Y （2005） cDNA cloning of the major yolk protein in sea urchin,
Strongylocentrotus intermedius.
The 1st International Symposium, “ Potential and Perspective of
st
Japan.
91.
Miyashita K (2005) Anti-carcinogenic activity of novel carotenoids from marine products.
Conference and Exhibition on Nutraceuticals and Functional Foods, Anaheim, USA.
92.
Miyashita K (2005) Anti-obesity effect of seaweed carotenoid.
Korean Society for Marine Biotechnology, Busan, Korea.
93.
Miyashita K, Beppu F and Hosokawa M (2005) Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic
acid on the growth of human cence cells. 26th World Congress and Exhibition of the International Society for Fat
Research, Prague Czech.
94.
Miyashita K, Hosokawa M, Kudo M, Maeda H, Kohno H and Tanaka T (2005) Seaweed carotenoid induces apoptosis
and enhances the antiproliferative effect of the PPARγ ligand on cancer cells. International Chemical Congress of
Pacific Basin Socoeties, Anaheim, USA.
95.
Miyashita K, Kinami T and Horri N (2005) Occurrence of conjugated trienoic acids in commercial soybean oil and
their impact on flavor reversion. 26th World Congress and Exhibition of the International Society for Fat Research,
Prague, Czech.
96.
Miyashita K, Kinami T, Horri N, Negishi H, Shirasawa S, Ikuina J and Noda R (2005) Trans fatty acid with conjugated
trienes induce flavor reversion and oxidation of commercial soybean oil. 96th AOCS Annual Meeting & Expo, Salt
Lake City, USA.
97.
Miyashita K, Maeda H, Hosokawa M and Sashima T (2005) Fucoxanthin from edible seaweed shows anti-obesity
effect through UCP1 expression in white adipose tissues. International Chemical Congress of Pacific Basin Socoeties,
Honolulu, USA.
98.
Miyashita K, Maeda H, Sashima T and Hosokawa M (2005) Antiobesity effect of seaweed carotenoid. 6th
International Conference and Exhibition on Nutraceuticals and Functional Foods, Anaheim, USA. （Invited）
99.
Miyashita K, Tsubakihara Y and Cesarettin A (2005) Antioxidant and anti-obesity effects of hazelnut polyphenols. 6th
International Conference and Exhibition on Nutraceuticals and Functional Foods, Anaheim, USA.
96th AOCS
6th International
International Symposium for Inauguration of The
100. Mori T, Saito S, Kishioka C and Arai K (2005) Comparative performance in diploid and triploid barfin flounder
(Verasper moseri). The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st
COE Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo, Japan.
101. Mori T, Saito S, Kishioka C and Arai K (2005) Sex ratios and growth performance of gynogenetic diploid barfin
flounder Verasper moseri. 6th International Symposium on Flatfish Ecology, Maizuru, Japan.
79
102. Morishima K, Ha TTT, Murakami T, Akashige S, Kajihara T, Umino T, Nishibori M, Arai K and Nakagawa H (2005)
Identification of culture population of Pacific oyster Crassostrea gigas by microsatellite DNA analysis. The 2nd
International Symposium. “Marine resources as functional and therapeutic agents”, 21st COE Program “Marine
Bio-Manipulation Frontier for Food Production, Sapporo, Japan.
103. Morishima K, Mori T, Kishioka C, Lahrech Z, Yoshikawa H, Saito S and Arai K (2005) Verification of induced
gynogenesis in barfin flounder, Verasper moseri by microsatellite DNA markers. 6th International Symposium on
Flatfish Ecology, Maizuru, Japan.
104. Morishima K, Yoshikawa H and Arai K (2005) Unreduced diploid sperm produced by artificially sex-reversed clonal
loaches. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”, 21st COE
105. Motomura T (2005) Cytological study on the brown algae, especially on the cytoplasmic inheritance of organelles.
Esil 2005: First symposium on the biology of the model brown alga Ectocarpus siliculosus, Brittany, France. （Invited）
106. Motomura T, Nagasato C and Mueller DG (2005) Ultrastructural studies on flagellar heterogeneity in zoospores of
Ectocarpus (Pheophyceae). 8th International Phycological Congress, Durban, South Africa.
107. Nagasato C, Kato A and Motomura T (2005) Destruction of maternal centrioles and reduction of centrin molecules in
brown algae. 8th International Phycological Congress, Durban, South Africa.
108. Nagasato C, Yoshikawa S, Kawai H and Motomura T (2005) New pyrenoid formation in brown algae.
Asian Pacific Phycological Forum, Bangkok, Thailand. （Invited）
The Fourth
109. Nakano D, Ura K, Shimizu M and Takagi Y (2005) Histological studies of the digestive system of Strongylocentrotus
intermedius. 21st COE the 1st International Symposium, Sapporo, Japan.
110. Nakayama D, Kawasaki T, Todo T, Adachi S and Yamauchi K (2005) Algae as purifiers of rearing water in closed
recirculating aquaculture systems.
The 4th International Symposium “Reproductive, Genetic and Disease
111. Nishizawa T, Kinoshita S and Yoshimizu M (2005) Genogrouping of Japanese isolates of aquabirnaviruses based on the
VP”/NS junction region. The 5th Joint Seminar between Korea and Japan by KOSEF・JSPS Core Univ. Program on
Fisheries Sciences-Zero Emission Fishery Industry: Present and Future-, Kunsan, Korea.
112. Oda A, Okawa K, Nakayama A, Urishibara N, Sasaki T, Miyazaki H, Wada I and Fujita H (2005) Cortactin regulates
the localization of WASP in platelets. 78th Meeting of Japan Society of Biochemistry, Kobe, Japan.
113. Ogawa K, Igarashi K, Fujita H, Shibahara S and Yanagisawa T (2005) Regulation of NO donor-mediated heme
oxygenase-1 induction by hypoxia in human lung cells. 78th Meeting of Japan Society of Biochemistry, Kobe, Japan.
114. Ogawa N, Ohira Y, Shimizu M, Ura K and Takagi Y (2005) Regenerating scale of goldfish: a new model for bone
calcificarion and resorption. 21st COE the 1st International Symposium, Sapporo, Japan.
115. Ooe H, Taira T, Iguchi-Ariga SMM and Ariga H (2005) Induction of reactive oxygen species by bisphenol A and
abrogation of bisphenol A-induced cell injury by DJ-1. The 4th International Symposium “Reproductive, Genetic and
116. Ootsuka S, Saga N and Ojima T (2005) Isolation and Characterization of a Novel endo-β-1,4-Mannanase from the
Pacific Abalone Haliotis discus hannai. 7th international marine biotechnology conference, St. John’s, Canada.
117. Saga N (2005) Cooperative Research Outline. Udayana University (2005). Anniversary Opening Symposium and
Workshop on Marine Biotechnology Signing MOU Between Udayana University, Hokkaido University and KYOWA
Concrete Company, Japan, Bali, Indonesia.
118. Saga N (2005) New project: marine bio-manipulation project. The 3rd International Symposium, “Industrial
Academia-Governmental Collaboration for the Establishment of Marine Food Production in China and Japan”, 21st
COE Program “Marine Bio-Manipulation Frontier for Food Production” as Commemorative Program for Agreement of
Academic Exchange between Shanghai Fisheries University and Hokkaido University, Shanghai, China.
119. Sago Y, Shin D-H, Haraguchi I, Unuma T, Kurokawa T, Nomura K, Matsubara H, Tanaka H, Kobayashi T, Kobayashi
H, Nagahama Y, Yamashita M, Todo T, Adachi S and Yamauchi K (2005) The cause of deterioration in egg quality of
the Japanese eel and goldfish. The 4th International Symposium “Reproductive, Genetic and Disease Management in
80
Hakodate, Japan.
120. Saito T, Arai K and Yamaha E (2005) Visualization of primordial germ cells in various bony fish embryos. The 1st
121. Sakao S, Fujimoto T, Yamaha E and Arai K (2005) Characteristics of primordial germ cells and the cause of larval
mortality in tetraploid masu salmon induced by the first cleavage inhibition. The 1st International Symposium,
122. Sasaki T, Oda A, Okawa K, Nakayama A, Urishibara N, Miyazaki H, Wada I, Nose K and Fujita H (2005) The
association of Nck with GIT1. 78th Meeting of Japan Society of Biochemistry, Kobe, Japan.
123. Sawaguchi S, Kagawa H, Ohkubo N, Hiramatsu N, Sullivan CV, Hara A and Matsubara T (2005) Molecular
characterization of three forms of vitellogenin and their yolk protein products during oocyte growth and maturation in
red seabream, Pagrus major. The 4th International Symposium “Reproductive, Genetic and Disease Management in
Hakodate, Japan. （Best Poster Award）
124. Shimazaki K, (2005) Proposed models for explaining multi-functionality of lactoferrin.
on Lactoferrin, Honolulu, USA.
7th International Conference
125. Shimizu K, Tosaka R, Kazeto Y, Todo T, Adachi S and Yamauchi K (2005) Effect of sex steroid hormones on the
expression of pituitary gonadotropins in previtellogenic female Japanese eel (Anguilla japonica). The 4th International
Symposium “Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching”, 21st COE
Program “Marine Bio-Manipulation Frontier for Food Production”, Hakodate, Japan.
126. Shimizu M, Fukada H, Hara A and Dickhoff WW (2005) Response of the salmon somatotropic axis to growth hormone
administration under two different salinities. The 7th International Workshop on Salmonid Smoltification, Tono,
Japan.
127. Shimizu M, Hara A and Dickhoff WW (2005) Measuring salmon insulin-like growth factor (IGF)-I and IGF-binding
proteins by radioimmunoassay. 15th International Congress of Comparative Endocrinology, Boston, USA.
128. Shin D-H, Haraguchi I, Todo T, Adachi S and Yamauchi K (2005) Correlation between egg quality and thyroid
hormone level in artificially matured Japanese eel. The 2nd International Symposium, “Marine resources as functional
and therapeutic agents”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo, Japan.
129. Sullivan CV, Couch CR, Garber AF, Hiramatsu N, Hiramatsu K, Rexroad III C and Westerman MA (2005) Hybrid
striped bass farming in the United States: Research and industry development. The 4th International Symposium
“Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching”, 21st COE Program “Marine
130. Suzuki H and Ojima T (2005) Isolation of an oligomannuronate lyase from the Pacific abalone Haliotis discus hannai.
7th International Marine Biotechnology Conference, St. John’s, Canada.
131. Suzuki H, Tanaka H, Ohtsuki I and Ojima T (2005) Functional analysis of NH2- and COOH-terminal regions of
Akazara scallop troponin-T. International symposium celebrating the 40th anniversary of the discovery of troponin,
Okazaki, Japan.
132. Suzuki K and Ojima T (2005) Cellulose degradation by abalone cellulases, HdEG66 and HdEG54.
Marine Biotechnology Conference, St. John’s, Canada.
7th International
133. Takagi Y (2005) Physiology of marine invertebrates: a molecular approach. The 3rd International Symposium, “
Industrial Academia-Governmental Collaboration for the Establishment of Marine Food Production in China and Japan
”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production” as Commemorative Program for
Agreement of Academic Exchange between Shanghai Fisheries University and Hokkaido University, Shanghai, China.
134. Takagi Y (2005) Teleost fish scales: a unique biological model to fabricate materials for corneal stroma regeneration.
International Symposium on Soft-Nanotechnology 2005 (ISSN2005), Sapporo, Japan.
135. Takagi Y, Ura K, Abe M and Suzuki A (2005) Teleost fish regenerating scales:a unique model of regenerating dermal
bone and corneal stroma. The 1st International Symposium, “Potential and Perspective of Marine-Bio-manipulation”,
136. Takagi Y, Ura K, Hasegawa M and Suzuki A (2005) Teleost fish regenerating scales: a unique model of regenerating
81
dermal bone and cornea stroma. The 2nd International Symposium, “Marine resources as functional and therapeutic
agents”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”, Sapporo, Japan.
137. Tanaka H, Takeya Y, Doi T, Yumoto F, Tanokura M, Ohtsuki I, Nishita K and Ojima T (2005) Regulatory roles of
NH2-terminal region of molluscan troponin-I. International symposium celebrating the 40th anniversary of the
discovery of troponin. Okazaki, Japan.
138. Tega T, Kim SG, Fujimoto T, Sato N, Fujioka T, Saito S and Arai K (2005) Induction of gynogenetic diploid and
triploid brown sole, Pleuronectes herzensteini. 6th International Symposium on Flatfish Ecology, Maizuru, Japan.
139. Those H, Ura K, Takagi Y and Nagasawa H (2005) Identification of starmaker-like protein gene and its possible
function on inner ear development and otolith biomineralization. The 4th International Symposium “Reproductive,
Genetic and Disease Management in Aquaculture and Ocean Ranching ” , 21st COE Program “ Marine
140. Tomimatsu R (2005) Algatech Kyowa: technical laboratory of seaweeds. The 3rd International Symposium,
“Industrial Academia-Governmental Collaboration for the Establishment of Marine Food Production in China and
Japan”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production” as Commemorative Program for
Agreement of Academic Exchange between Shanghai Fisheries University and Hokkaido University, Shanghai, China.
141. Tosaka R, Todo T, Adachi S and Yamauchi K (2005) Localization of androgen receptor mRNA in gonads of Japanese
eel (Anguilla japonica). The 4th International Symposium “Reproductive, Genetic and Disease Management in
Hakodate, Japan.
142. Tun KM, Akuzawa R, Kumura H, Tanaka T and Shimazaki K, (2005) Lactoferrin and lactoferrin-derived peptides in
cheese during ripening period. 4th NIZO Dairy Conference, Papendal, The Netherlands.
143. Uchiyama J, Ohno H, Arai K and Abe S (2005) Isolation and characterization of molecular cytogenetic markers in
Japanese flounder. 6th International Symposium on Flatfish Ecology, Maizuru, Japan.
144. Ueki C, Nagasato C, Motomura T and Saga N (2005) Development and motility of spores in Porphyra yezoensis
(Bangiales, Rhodophyta). The Fourth Asian Pacific Phycological Forum, Bangkok, Thailand.
145. Uemori C, Nagasato C, Kato A and Motomura T (2005) Cytological study on mitosis and flagellar duplication in
Ochromonas. The Fourth Asian Pacific Phycological Forum, Bangkok, Thailand.
146. Ura K, Miyake M, Koga Y, Todo T, Hara A and Takagi Y (2005) Immunochemical and biochemical studies on egg yolk
protein in sea urchin, Strongylocentrotus intermedius. The 4th International Symposium “Reproductive, Genetic and
147. Ura K, Miyake M, Koga Y, Todo T, Hara A and Takagi Y (2005) Immunochemical and biochemical studies on egg yolk
protein in sea urchin, Strongylocentrotus intermedius. 2nd Japan-Korea, Korea-Japan Joint Meeting on Reproductive
Biology of Aquatic Animals, Kumamoto, Japan.
148. Wada T, Amano H, Fujita T, Soyano K and Hara A (2005) Estrogenic impacts in coastal aquatic environments: Surveys
on serum vitellogenin levels in the grey mullet and the red lip mullet. The 2nd Seminar of JSPS Multilateral Core
University Program on “Coastal Oceanography”, Tokyo, Japan.
149. Wada T, Amano H, Fujita T, Soyano K, Yeo I-K and Hara A (2005) Serum vitellogenin levels in grey mullet and red lip
mullet. The 4th International Symposium “Reproductive, Genetic and Disease Management in Aquaculture and Ocean
Ranching”, 21st COE Program “Marine Bio-Manipulation Frontier for Food Production”, Hakodate, Japan.
150. Wongchawalit J, Chanchao C, Okuyama M, Mori H, Suralit R, Svasti MRJ, Chiba S and Kimura A (2005) Purification
and characterization of α-glucosidase I from Thai honeybee (Apis florea). 54th Meeting of the Japanese Society of
Applied Glycoscience, Tsu, Japan.
151. Yamaguchi N, Ashida M, Kobayashi Y, Todo T, Adachi S, Yamauchi K and Nakamura M (2005) In vitro induction of
gonadal sex change in protogynous fish. The 4th International Symposium “Reproductive, Genetic and Disease
152. Yamaha E (2005) Developmental bio-technology for aquaculture, with special reference to surrogate production in
teleost fishes. 6th International Symposium on Flatfish Ecology, Maizuru, Japan.（Invited）
153. Yamauchi K (2005) Position formation for the promotion of the fisheries industry and talent reading in the Asia Pacific
82
Regions. Udayana University 2005 Anniversary Opening Symposium and Workshop on Marine Biotechnology
Signing MOU Between Udayana University, Hokkaido University and KYOWA Concrete Company, Japan, Bali,
Indonesia.
154. Yoon M, Sato S, Seeb JE, Brykov V, Wilmot RL, Varnavskaya N, Jin D-H, Urawa S, Urano A and Abe S (2005)
Genetic variation among chum salmon populations in the Pacific Rim inferred from the mitochondrial and
microsatellite DNA analyses. The 5th Joint Seminar between Korea and Japan by KOSEF・JSPS Core Univ. Program
on Fisheries Sciences-Zero Emission Fishery Industry: Present and Future-, Kunsan, Korea.
155. Yoon M, Sato Si, Urawa S, Urano A and Abe S (2005) Genetic variation and population structure of chum salmon
inferred from the mitochondrial and microsatellite DNA analyses. Joint-Annual Meeting between Hokkaido and
Tohoku Chapters of Japan Society of Fisheries Science, 2005, Sendai, Japan.
156. Yoshida Y, Ura K, Shimizu M, Sakurai Y and Takagi Y (2005) Development of the statocyst and statolith in the
Japanese common squid, Tadarodes pacificus: ultrastructural observations. The 1st International Symposium,
157. Yoshimizu M (2005) Disease problems and their control in aquaculture –A control strategy for viral diseases in
salmonids and flounder-. The 3rd International Symposium, “Industrial Academia-Governmental Collaboration for the
Establishment of Marine Food Production in China and Japan”, 21st COE Program “Marine Bio-Manipulation Frontier
for Food Production” as Commemorative Program for Agreement of Academic Exchange between Shanghai Fisheries
University and Hokkaido University, Shanghai, China.
158. Yoshimizu M and Kasai H (2005) Disinfection of Seawater for Aquaculture by an Electrolytic Apparatus and Its
Application to Fisheries Sanitation. The JSPS-NRCT International Symposium Joint Seminar 2005, Bangkok,
Thailand.
159. Yoshimizu M, Kasai H and Shimizu T (2005) Survivability of koi herpesvirus (KHV) in environmental water as well as
physical and chemical inactivation. KHV Workshop, The 12th International Conference on “Disease of Fish and
Shellfish”, Copenhagen, Denmark.
160. Yoshimizu M, Kasai H, Shimizu T, Matsuda N and Watanabe K (2005) Biological control of fish viral diseases with
anti-viral substances produced by bacteria. The 12th International Conference on “Disease of Fish and Shellfish”,
Copenhagen, Denmark.
161. Yoshimizu M, Kasai H, Shimizu T, Matsuda N and Watanabe K (2005) Biological control of fish viral diseases with
anti-viral substances produced by bacteria. The 4th International Symposium “Reproductive, Genetic and Disease
Other 193 presentations in domestic meetings.
83
Original Articles
1.
Azuma N, Kunihiro Y, Sasaki J, Nozawa Y, Mihara E, Mihara Y, Yasunaga T and Abe S (2006) Genetic variation of Hair
Crab (Erimacrus isenbeckii）inferred from mitochondrial DNA sequence analysis. Fish Genet. Breed Sci 35: 35-42.
2.
Beppu F, Hosokawa M, Tanaka L, and Miyashita K (2006) Potent inhibitory effect of trans9, trans11 isomer of
conjugated linoleic acid on the growth of human colon cancer cells. J Nutr Biochem 17: 830-836.
3.
Elsheikh AS, Takahashi Y, Katagiri S, Kanagawa H (2006) Sucrose-exposed chemically enucleated mouse oocytes
support blactocyst development of reconstituted embryos. Reprod Fert Dev 18: 697-701.
4.
Endo H, Ootsuka S, Fukuda S, Kitade Y and Saga N (2006) Functional complementation of an arginene auxotrophic
yeast mutant by an argininosuccinate synthetase from Porphyra yezoensis (Rhodophyta). J Phycol 42: 1066-1071.
5.
Fujimoto T, Kataoka T, Sakao S, Saito T, Yamaha E, and Arai K (2006) Developmental stages and germ cell lineage of
the loach (Misgurnus anguillicaudatus). Zool Sci 23: 977-989.
6.
Goto-Kazeto R, Abe Y, Masai K, Yamaha E, Adachi S and Yamauchi K (2006) Temperature-dependent sex
differentiation in goldfish: establishing the temperature-sensitive period and effect of constant and fluctuating water
temperatures. Aquaculture 254: 617-624.
7.
Hagio Y, Kimura Y, Taira T, Fuijioka Y, Iguchi-Ariga SMM and Ariga H (2006) Distinct localizations and repression
activities of MM-1 isoforms toward c-Myc. J Cell Biochem 97: 145-155.
8.
Hirayama H, Kageyama S, Takahashi Y, Moriyasu S, Sawai K, Onoe S, Watanabe K, Kojiya S, Notomi T,
Minamihashi A (2006) Rapid sexing of water buffalo (Bubalus bubalis) embryos using loop-mediated isothermal
amplification. Theriogenology 66: 1249-56.
9.
Ijiri S, Takei N, Kazeto Y, Todo T, Adachi S and Yamauchi K (2006) Changes in localization of cytochrome P450
cholesterol side-chain cleavage (P450scc) in Japanese eel testis and ovary during gonadal development. Gen Comp
Endocrinol 145: 75-83.
10.
Inden M, Taira T, Kitamura Y, Yanagida T, Tsuchiya D, Takata K, Yanagisawa D, Nishimura K, Taniguchi T, Kiso Y,
Yoshimoto Y, Agatsuma T, Koide-Yoshida S, Iguchi-Ariga SMM, Shimohama S and Ariga H (2006) PARK7 DJ-1 protects
against degeneration of nigral dopaminergic neurons in Parkinson’s disease rat model. Neurobiol Dis 24: 144-158.
11.
Ishizaki R, Shin H-W, Iguchi-Ariga SMM, Ariga H and Nakayama K (2006) AMY-1 (Associate of Myc-1) localization
to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation
factors. Genes Cells 11: 949-959.
12.
Itono M, Morishima K, Fujimoto T, Yamaha E, and Arai K (2006) Premeiotic endomitosis produces diploid eggs in the
natural clone loach, Misgurnus anguillicaudatus (Teleostei:Cobitidae). J Exp Zool 305A: 513-523.
13.
Jiang X, Morita M, Sugioka A, Harada ., Kojo S, Wakao H, Watarai H, Ohkohchi N, Taniguchi M and Seino KI (2006) The
importance of CD25(+)CD4(+) regulatory T cells in mouse hepatic allograft tolerance. Liver Transpl 12: 1112-1118.
14.
Kai T, Nimura K, Yasui H and Mizuta H (2006) Regulation of sorus formation by auxin in Laminaria japonica
(Phaeophyceae). J Appl Phycol 18: 95-101.
15.
Katagiri S, Takahashi Y (2006) Effect of normalization of epidermal growth factor profile in the endometrium on
restoration of fertility in repeat breeder cows. Anim Reprod Sci 95: 54-66.
16.
Kato Y, Kogame K, Nagasato C and Motomura T (2006) Inheritance of mitochondrial and chloroplast genomes in the
isogamous brown alga Scytosiphon lomentaria (Phaeophyceae). Phycological Research 54: 65-71.
17.
Kawakami, Y, Shin D-H, Kitano T, Adachi S, Yamauchi K and Ohta H (2006) Transactivation activity of thyroid hormone
receptors in fish (Conger myriaster) in response to thyroid hormones. Comp Biochem Physiol B 144: 503-509.
18.
Kazeto Y, Ijiri S, Adachi S and Yamauchi K (2006) Cloning and characterization of a cDNA encoding cholesterol
side-chain cleavage cytochrome P450 (CYP11A1): Tissue-distribution and changes in the transcript abundance in
ovarian tissue of Japanese eel, Anguilla japonica,during artificially induced sexual development. J Steroid Biochem
Mol Biol 99: 121-128.
19.
Kim D, Kang HK, Seo ES, Kim GJ, Kimura A (2006) Potential industrial application of glycosyltransferases and their
evolutions. J Biomed Nanotechnol 2: 120–124.
84
20.
Kim W-S, Shimazaki K and Tamura T (2006) Expression and characterization of recombinant bovine lactoferrin C-lobe
in Rhodococcus erythropolis. Biosci Biotech Biochem 70(11): 2641-2645.
21.
Kim Y-M, Lee G-H, Yeo Y-G, Kim I-H, Miyashita K, Hou C-H, Kang S-C, Kim H-R (2006) The effect of bio-converted
polyunsaturated fatty acids on the oxidation of TAG containing highly unsaturated fatty acids. J Ind Microbiol
Biotechnol 33: 17-21.
22.
Kimura T, Yasui H and Mizuta H (2006) Nutrient requirement for zoospore formation in two Alariaceae plants, Undaria
pinnatifida (Harvey) Suringar and Alaria crassifolia Kjellman (Phaeophyceae: Laminariales). Fish Sci 72: 860-869.
23.
Kitamura S-I, Jung S-J, Kim W-S, Nishizawa T, Yoshimizu M, and Oh M-JA (2006) new genotype of
lymphocystisvirus, LCDV-RF, from lymphocystis diseased rockfish. Arch Virol 151: 607-615.
24.
Konishi I, Hosokawa M, Sashima T, Kobayashi H, and Miyashita K (2006) Halocynthiaxanthin and fucoxanthinol isolated from
Halocynthia roretzi induce apoptosis in human leukemia, breast and colon cancer cells. Comp Biochem Physiol 142: 53-59.
25.
Kumura H, Minato N and Shimazaki K (2006) Inhibitory activity of bovine milk osteopontin and its fragments on the
formation of calcium phosphatge precipitates. J Dairy Res 73: 449-453.
26.
Kuwaki T, Oda A, Yuki C, Suzuki H, Murasaki K, Fujita H, Miyzaki H and Ikeda Y (2006) Lineage-Specific Expression of
G-CSF and TPO Receptors in Terminally-Differentiated Hematopoietic Cells. Exp Hematol 34(12): 1651-1654.
27.
Li HM, Taira T, Maita C, Ariga H and Iguchi-Ariga SMM (2006) Protection against nonylphenol-induced cell death
by DJ-1 in cultured Japanese medaka (Oryzias latipes) cells. Toxicology 228: 229-238.
28.
Maeda H, Hosokawa M, Sashima T, Takahashi N, Kawada T, and Miyashita K (2006) Fucoxanthin and its metabolite,
fucoxanthinol, suppress sdipocyte differentiation in 3T3-L1 cells. Int J Mol Med 18: 147-152.
29.
Mahmoud BSM, Kawai Y, Yamazaki K, Miyashita K, and Suzuki T (2006) A new technology of fish preservation by
combined treatment with electrolyzed NaCl solutions and essential oil compounds. Food Chem, 99: 656-662.
30.
Malcuit C, Maserati M, Takahashi Y, Page R, Fissore RA (2006) Intracytoplasmic sperm injection in the bovine
induces abnormal [Ca2+]i responses and oocyte activation. Reprod Fertil Dev 18: 39-51.
31.
Mori T, Saito S, Kishioka C, and Arai K (2006) Aquaculture performance of triploid barfin flounder Verasper moseri.
Fish Sci 72(2): 270-277.
32.
Morshedur Rahman Md, Kim W-S, Tanaka T, Kumura H and Shimazaki K (2006) Lactoferrin effects on the growth
of bifidobacteria. Food & Food Ingred J Jpn 211(9): 763-770.
33.
Nagai K, Takikawa O, Kawakami N, Fukao M, Soma T, Oda A, Nishiya T, Hayashi M, Lu L, Nakano M, Kajita E,
Fujita H and Miwa S (2006) Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine
transporter, OCTN2. Arch Biochem Biophys 452: 29-37.
34.
Nagano M, Katagiri S, Takahashi Y (2006) ATP content and maturational/developmental ability of bovine oocytes with
various cytoplasmic morphologies. Zygote 14: 299-304.
35.
Nagano M, Katagiri S, Takahashi Y (2006) Relationship between bovine oocyte morphology and in vitro developmental
potentail. Zygote 14: 53-61.
36.
Nakai H, Okuyama M, Mori H, Chiba S, Sano Y, and Kimura A (2006) Plant α-glucosidase: molecular analysis of rice
α -glucosidase and degradation mechanism of starch granule in germination stage. J Appl Glycosci 53: 137-142.
37.
Nishizawa T, Kinoshita S, Kim W-S, Higashi S and Yoshimizu, M. (2006) Nucleotide diversity of Japanese isolates of
infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene. Dis Aquat Org 71: 267-272.
38.
Nishizawa T, Savas H, Ishidan H, Ustundand C, Iwamoto H and Yoshimizu M. (2006) Genotyping and pathogenicity of
viral hemorrhagic septicemia virus from free-living turbot (Psetta maxima) in a Turkish coastal area of the Blak Sea.
Appl Environ Microbiol 72: 2373-2378.
39.
Nomura K, Morishima K, Tanaka H, Unuma T, Okuzawa K, Ohta H, and Arai K (2006) Microsatellite - centromere mapping
in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families. Aquaculture 257: 53-67.
40.
Okuyama M, Kaneko A, Mori H, Chiba S, and Kimura A (2006) Structural elements to convert α-xylosidase into
α -glucosidase. FEBS Lett 580: 2707-2711.
41.
Ono S, Hosokawa M, Miyashita K, and Takahashi K (2006) Inhibition properties of dipeptides from salmon muscle
hydrolysate on angiotensin I-converting enzyme. Int J Food Sci Tech 41: 383-386.
42.
Ooe H, Iguchi-Ariga SMM and Ariga H (2006) Establishment of specific antibodies that recognize C106-oxidized DJ-1.
85
Neuroscience Lett 404: 166-169.
43.
Ooe H, Maita C, Maita H, Iguchi-Ariga SMM and Ariga H (2006) Specific cleavage of DJ-1 under an oxidative
condition. Neuroscience Lett 406: 165-168.
44.
Ootsuka S, Saga N, Suzuki K, Inoue A and Ojima T (2006) Isoation and cloning of an endo-β-1,4-mannanase from
pacific abalone Haliotis discus hannai. J Biotecnol 125: 269-280.
45.
Ozaki Y, Fukada H, Kazeto Y. Adachi S, Hara A and Yamauchi K (2006) Molecular cloning and characterization of growth
hormone receptor and its homologue in the Japanese eel (Anguilla japonica) Comp Biochem Physiol B, 143(4): 422-431.
46.
Ozaki Y, Fukada H, Tanaka H, Kagawa H, Ohta H, Adachi S, Hara A and Yamauchi K (2006) Changes in the
expression of growth hormone family and the growth hormone receptor during early development in the Japanese eel
(Anguilla japonica) Comp Biochem Physiol B 145: 27-34.
47.
Ozaki Y, Tanaka H, Kagawa H, Ohta H, Adachi S and Yamauchi K (2006) The fine structure and differentiation of the
alimentary canal of preleptocephali of Japanese eel, Anguilla japonica. Fisheries Sci 72: 13-19.
48.
Qu X-C, Shin D-H, Nagae M, Todo T, Adachi S and Yamauchi K (2006) Changes in serum thyroid hormone levels and
thyroid gland activity in artificially maturing female Japanese eel (Anguilla japonica). Aquaculture Sci, 54: 283-292.
49.
Saburi W, Mori H, Saito S, Okuyama M, and Kimura A (2006) Structural elements in dextran glucosidase responsible
for high specificity to long chain substrate. Biochim Biophys Acta 1764: 688-698.
50.
Saika O, Kohayakawa Y and Hara A (2006) Effects of tributyltin on ephippa production in Daphnia magna. Jpn J
Environ Toxicol 9(1): 1-9.
51.
Saito T, Fujimoto T, Maegawa S, Inoue K, Tanaka M, Arai K, and Yamaha E (2006) Visualization of primordial germ
cells in vivo using GFP-nos1 3’UTR mRNA. Int J Dev Biol 50: 691-699.
52.
Sakaguchi M, Sasamoto Y, Suzuki T, Takahashi Y, Yamada Y (2006) Fate of cystic ovarian follicles and the
subsequent fertility of early postpartum dairy cows. Vet Rec 159: 197-201.
53.
Sakao S, Fujimoto T, Kimura S, Yamaha E, and Arai K (2006) Drastic mortality in tetraploid induction results from the
elevation of ploidy im masu salmon Oncorhynchus masou. Aquculture 252: 147-160.
54.
Sawada Y, Hattori M, Iteya M, Takagi Y, Ura K, Seoka M, Kato K, Kurata M, Miyatake H, Katayama S and Kumai H (2006)
Induction of centrum defects in amberjack, Seriola dumerili, by exposure of embryos to hypoxia. Fish Sci 72: 364-372.
55.
Sawada Y, Hattori M, Sudo N, Kato K, Takagi Y, Ura K, Kurata M, Okada T and Kumai H (2006) Hypoxic conditions
induce centrum defects in red sea bream Pagrus major (Temminck and Schlegel). Aqua Res 37: 805-812.
56.
Sawaguchi S, Kagawa H, Ohkubo N, Hiramatsu N, Sullivan CV and Matsubara T (2006) Molecular characterization of
three forms of vitellogenin and their yolk protein products during oocyte growth and maturation in red seabream
(Pagrus major), a marine teleost spawning pelagic eggs. Mol Reprod Dev 73: 719-736.
57.
Sekito A, Koide-Yoshida S, Niki T, Taira T, Iguchi-Ariga SMM and Ariga H (2006) DJ-1 interacts with HIPK1 and
affects H2O2-induced cell death. Free Radic Res 40: 155-165.
58.
Shimizu M, Beckman B, Hara A and Dickhoff WD (2006) Measurement of circulating salmon IGF binding protein-1: assay
development, response to feeding ration and temperature, and relation to growth parameters. J Endocrinol 188: 101-110.
59.
Shimizu T, N Yoshida H Kasai and M Yoshimizu (2006) Survival of koi herpesvirus (KHV) in environmental water.
Fish Pathol 41: 153-157.
60.
Shinbo Y, Niki T, Taira T, Ooe H, Takahashi-Niki K, Maita C, Seino C, Iguchi-Ariga SMM and Ariga H (2006) Proper
SUMO-1 conjugation is essential to DJ-1 to exert its full activities. Cell Death Diff 13: 96-108.
61.
Suetsugu S, Kurisu S, Oikawa T, Yamazaki D, Oda A and Takenawa T (2006) Optimization of WAVE2
complex-induced actin polymerization by membrane-bound IRSp53, PIP(3), and Rac. J Cell Biol 173(4):5 71-85.
62.
Suzuki H, Suzuki K, Inoue A and Ojima T (2006) A novel oligoalginate lyase from abalone, Haliotis discus hannai,
that releases disaccharide from alginate polymer in an exolytic manner. Carbohydr Res 341: 1809-1819.
63.
Suzuki R, Yasui Y, Kohno H, Miyamoto S, Hosokawa M, Miyashita K, and Tanaka T (2006) Catalpa seed oil rich in
9t,11t,13c-conjugated linolenic acid suppresses the development of colonic aberrant crypt foci induced by
azoxymethane in rats. Oncology Re 16: 989-996.
64.
Tanaka T, Murakamai S, Kumura H, Igarashi I and Shimazaki K (2006) Parasiticidal activityt of bovine lactoperoxidase
against Toxoplasma gondii. Biochem. Cell Biol 84: 774-779.
86
65.
Those H, Murayama E, Ohira T, Takagi Y and Nagasawa H (2006) Localization and diurnal variations of carbonic anhydrase
mRNA expression in the inner ear of the rainbow trout Oncorhynchus mykiss. Comp Biochem Physiol B 145: 257-264.
66.
Tomimatsu R, Takahashi M, Endo H, Kitade Y, Yasui H and Saga N (2006) Induction and characterization of a brilliant
green mutant in a marine red alga Porphyra yezoensis (Bangiales, Rhodophyta). Fish Genet Breed Sci 36: 43-47.
67.
Uemori C, Nagasato C, Kato A and Motomura T (2006) Ultrastructural and immunocytological studies on the
rhizoplast in the chrysophycean algae Ochromonas danica. Phycological Research 54: 133-139.
68.
Wongchawalit J, Yamamoto T, Nakai H, Kim Y-M, Sato N, Nishimoto M, Okuyama M, Mori H, Saji O, Chanchao C,
Wongsiri S, Surarit R, Svasti J, Chiba S and Kimura A (2006) Purification and characterization of α-glucosidase I from
Japanese honeybee (Apis cerana japonica), and molecular cloning of its cDNA. Biosci Biotechnol Biochem 70: 2889-2898.
69.
Yamaki M, Yamaguchi S, and Arai K (2006) Mottled coloration of haploid-diploid and diploid-triploid mosaic amago
salmon Oncorhynchus masou ishikawae. Fish Sci 72(1): 157-165.
70.
Yamamoto T, Terasawa K, Kim YM, Kimura A, KitamuraY, Kobayashi M, and Funane K (2006) Identification of catalytic
amino acids of cyclodextran glucanotransferase from Bacillus circulans T-3040. Biosci Biotechnol Biochem 70: 1947-1953.
71.
Yamamoto Y, Hosokawa M, and Miyashita K (2006) Production of phosphatidylcholine containing conjugated linoleic
acid mediated by phospholipase A2. J Mol Cata B: Enzymatic 41: 92-96.
72.
Yasui Y, Hosokawa M, Kohno H, Tanaka T, and Miyashita K (2006) Comparison of growth inhibition and apoptosis
induction by troglitazone and 9cis, 11trans, 13trans-conjugated linolenic acid on different colon cancer cell lines.
Chemotherapy 52: 220-225.
73.
Yasui Y, Hosokawa M, Kohno H, Tanaka T, and Miyashita K (2006) Growth inhibition and apoptosis induction by
all-trans-conjugated linolenic acids on human colon cancer cells. Anticancer Res 26: 1855-1860.
74.
Yasui Y, Hosokawa M, Kohno H, Tanaka T, and Miyashita K (2006) Troglitazone and 9cis,11trans,13trans-conjugated
linolenic acid: Comparison of their antiproliferative and apoptosis-inducing effects on different colon cancer cell lines.
Pharmacology 52: 220-225.
75.
Yoon M and Abe S (2006) Nucleotide sequence variation in the 3’ portion of the mitochondrial DNA control region of
chum salmon. Fish Genet Breed Sci 36: 63-67.
Other 12 papers published in domestic journals such as Nippon Suisan Gakkaishi, Suisanikusyu (Fish Genetics and Breeding
Science), Suisanzoshoku (Aquaculture Science) and others.
Reviews and Books
1.
Hiramatsu N, Matsubara T, Fujita T, Sullivan CV and Hara A (2006) Multiple piscine vitellogenins: biomarkers of fish
exposure to estrogenic endocrine disruptors in aquatic environments. Marine Biol 149: 35-47.
2.
Hosokawa M, Narayan B, Sashima T and Miyashita K (2006) Fucoxanthin as a bioactive and nutritionally beneficial
marine carotenoid: A review. Carotenoid Sci 10: 15-28.
3.
Miyashita K (2006) Seaweed carotenoid, fucoxanthin, with highly bioactive and nutritional activities. J Marine Biosci
1: 48-58.
4.
Narayan B, Hosokawa M and Miyashita K (2006) Occurrence of conjugated fatty acids in aquatic and terrestrial plants
and their physiological effects. In Nutraceutical and Specialty Lipids and Their Co-Products (Shahidi F ed), CRC
Taylor & Francis, New York, pp. 201-218.
5.
Narayan B, Miyashita K and Hosokawa M (2006) Physiological effects of eicosapentaenoic acid (EPA) and
docosahexaenoic acid (DHA) – A review. Food Rev Inter 22: 291-307.
6.
Rahman MdM, Kim W-S, Tanaka T, Kumura H and Shimzaki K (2006) Lactoferrin effects on the growth of
bifidobacteria, Foods Food Ingred. J Jpn 211(9) 763-768.
Other 22 review papers published in Japanese books and domestic journals such as Nippon Suisan Gakkaishi, Suisanikusyu
(Fish Genetics and Breeding Scienc) and others
87
Others
1.
Abe T, Todo T, Adachi S and Yamauchi Y (2006) In vitro oocyte maturation, fertilization and development in Japanese
eel, Anguilla japonica. Hokkaido University 21st Century COE Program; The 5th International Symposium Marine
Bio-Manipulation Frontier for Food Production, Abstract p53.
2.
Akiyama S, Todo T, Adachi S and Yamauchi Y (2006) Induction of vitellogenin synthesis in primary cultured
hepatocytes of Japanese eel (Anguilla japonica). Hokkaido University 21st Century COE Program; The 5th
International Symposium Marine Bio-Manipulation Frontier for Food Production, Abstract p54.
3.
Endo T, Todo T, Adachi S and Yamauchi Y (2006) In vitro induction of oil droplet accumulation into previtellogenic
oocytes of Japanese eel, Anguilla japonica. Hokkaido University 21st Century COE Program; The 5th International
Symposium Marine Bio-Manipulation Frontier for Food Production, Abstract p51.
4.
Iizuka S, Tosaka R, Kazeto Y, Todo T, Adachi S and Yamauchi Y (2006) Gonadal expression of progestin receptors a
and b mRNA during sexual maturation of Japanese eel, Anguilla japonica. Hokkaido University 21st Century COE
Program; The 5th International Symposium Marine Bio-Manipulation Frontier for Food Production, Abstract p52.
5.
Kim U, Nishizawa T and Yoshimizu M (2006) Evaluation of methods for sero- epidemiology and surveillance of
infectious hematopoietic necrosis (IHN). In Proceedings of the 5th Japan-Korea Joint Seminar on Fisheries Sciences,
Sep.29-30, 2005, Kunsan, Korea, pp. 190-194.
6.
Nishizawa T, Kinoshita S and Yoshimizu M (2006) Genogrouping of Japanese isolates of aquabirnaviruses based on the
VP2/NS junction region. In Proceedings of the 5th Japan-Korea Joint Seminar on Fisheries Sciences, Sep.29-30, 2005,
Kunsan, Korea, pp. 182-187.
7.
Tosaka R, Kazeto Y, Todo T, Adachi S and Yamauchi Y (2006) Induction of spontaneous vitellogenesis in Japanese eel.
Hokkaido University 21st Century COE Program; The 5th International Symposium Marine Bio-Manipulation Frontier
for Food Production, Abstract p50.
8.
Yoon M, Azuma N, Sato S, Seeb JE, Wilmot RL, Urawa S, Urano A and Abe S (2006) Genetic variation among Pacific
Rim chum salmon populations inferred from the microsatellite DNA analysis. NPAFC Document No. 964: pp. 1-20.
Other 27 papers published in Japanese
Patents
1.
2006-105849 by Miyashita et al.
2.
3.
2006-259295 by Wakao et al.
1.
Abe S, Yoon M and Azuma N (2006) Genetic management and sustainable use of marine bioresources. International
Symposium – How to sustain agrosphere, biosphere and geosphere, Hokkaido University International Symposium on
Sustainable Development Regular Session, Hokkaido University Conference Hall, August 8, 2006, Sapporo, Japan.
（Invited）
2.
Abe S, Yoon M, Sato S, Moriya S, Urawa S, and Urano A (2006) Genetic variation and population structure of chum
salmon in the North Pacific Rim inferred from mitochondrial and microsatellite DNA analyses. International
Symposium on Genetics in Aquaculture IX, IAGA, June 27, 2006, Montpellier, France.
3.
Amano H, Fujita T, Hiramatsu N, Hara A (2006) Purification of multiple vitellogenins from grey mullet. 5th
International Symposium of “Industrial-Academia-Governmental Collaboration for the Establishment of Marine
Production in China and Japan”, July 20-22, Hakodate, Japan.
4.
Amano H, Fujita T, Hiramatsu N, Shimizu M, Sawaguchi S, Matsubara T, Kagawa H, Sullivan CV, Hara A (2006)
88
Purification and classification of egg yolk proteins in grey mullet (Mugil cephalus). The 6th Joint Seminar between
Japan and Korea by Core University Program on Fisheries Sciences –Sustainability of Fisheris in Japan and Korea-,
August 28-29, Ohnuma International Seminar House, Hakodate, Japan.
5.
Aoki J, Nagae M, Takao Y, Hara A, Lee YD, Yeo IK, Lim BS, Park CB, Soyano K (2006) Effects of endocrine
disrupting chemicals using wild grey mullet at Korea and Japan. (abstract p. 21) Korea-Japan, Japan-Korea Joint
Meeting on Reproductive Biology of Aquatic Animals, November20-21, Cheju National University, Cheju, Korea
6.
Arai K (2006) Recent progress in aquaculture-oriented genomics and biotechnology. International Symposium on
Sustainable Use of Aquatic Resources, 60th Anniversaty of College of Fisheries, Ocean University of China, October
2006, OUC, Qingdao, China.
7.
Arai K (2006) Research Activities for advanced aquaculture studies in the division of marine life science, Symposium
on Developing Fisheries Science in Asia, December 2006, Shanghai Fisheries University, Shanghai, China.
8.
Choi S-J, Park E-J, Fukuda S, Endo H, Kitade Y and Saga N (2006) Porphyra yezoensis as a model plant in marine
bioscience – the state and prospect. Intl. Marine Algae & Global Warming, October 2006, R.O.K. Reichstag, Seoul,
Korea.
9.
Cook JM, Aiach N, Charrier B, Coelho S, Corre E, Farnham G, Kitade Y, Ratin M, Remblière, Scornet D, Segurens B,
Setterblad B, Weissenbach J, Wincker P and Peters A (2006) Progress on the Ectocarpus genome project. 54th Annual
Meeting of the British Phycological Society, January 2006, Plymouth, U.K.
10.
Endo H, Park E-J, Fukuda S, Kitade Y and Saga N (2006) Porphyra yezoensis: A model plant in marine bioscience. The
5th International Symposium Hakodate-Shanghai “Industrial-Academia-Governmental Collaboration for the
Establishment of Marine Food Production in China and Japan”, July 2006, Hokkaido University, Hakodate, Japan.
11.
Fujimoto T, Morishima K, Yoshikawa H, Yamaha E, and Arai K (2006) Development of sterile host by chromosome
manipulation and geno knock-down for the surrogate propagation using germ-line chimera. The 5th International
Symposium, Industrial-Academia-Governmental Collaboration for the Establishment of Marine Food Production in
Japan and China, July 2006, Hokkaido University, Hakodate, Japan.
12.
Funane K, Kawabata Y, Terasawa K, Kim Y-M, Kimura A, Nakai S, Kobayashi M (2006) Functions of the C-terminal
three domains of cycloisomaltooligosaccharide glucanotransferase (CITase). 3rd Food Protein Symposium, May 9 2006,
Yamaguchi University, Yamaguchi.
13.
Funane K, Kawabata Y, Terasawa K, Yamamoto T, Kim Y-M, Kimura A, Kobayashi M (2006) Identification of catalytic
amino acids and characterization of the C-terminal domains of cycloisomaltooligosaccharide glucanotransferase (CITase).
23rd International Carbohydrate Symposium, July 23-28 2006, Whistler Conference Center, Whistler, Canada..
14.
Goto-Kazeto R, Saito T, Arai K, and Yamaha E (2006) Efficient isolsation of primordial germ cells in zebrafish by cell
sorting, 7th International Conference on Zebrafish Development and Genetics, June 2006, University of
Wisconsin-Madison, USA.
15.
Hara A (2006) Aquaculture of Hucho perryi. Special lecture. October 19, Quighai Provincial Fishery Environmental
Monitoring Station, Quinghai, China.
16.
Hara A (2006) Fish vitellogenin as a biomarker for endocrine disruption. Special lecture in Udayana University and
Hokkaido University Workshop of Marine Biology (abstract p.9), September 22-24, Udayana University, Bali, Indonasia.
17.
Hara A (2006) Fish vitellogenin: our current understandings. Special lecture in 60th Anniversary of College of Fisheries,
International Symposium on Sustainable Use of Aquatic Resources (abstract p.16), October 16-18, Ocean University of
China, Qingtao, China.
18.
Hiramatsu N, Amano H, Fujita T, Hara A (2006) Development of monitoring system on endocrine disruption. 21st COE
Symposium on Developing Fisheries Science in Asia, December 18-19, Shanghai Fisheries University, Shanghai, China.
19.
Hiramatsu N, Amano H, Fujita T, Matsubara T, Todo T, Nagae M, Soyano K, Hara A (2006) Fish vitellogenin: single
versus multiple vitellogenin models. (abstract pp.17-18), Korea-Japan, Japan-Korea Joint Meeting on Reproductive
Biology of Aquatic Animals, November20-21, Cheju National University, Cheju, Korea.
20.
Hong L, Amano H, Fujita T, Shimizu M, Hara A (2006) Immunochemical detection of choryogenins (precursors to
vitelline envelope) in grey mullet (Mugil cephalus). 5th International Symposium of “Industrial-Academia-Governmental
Collaboration for the Establishment of Marine Production in China and Japan”, July 20-22, Hakodate, Japan.
21.
Hong L, Hiramatsu N, Amano H, Fujita T, Hara A (2006) Multiple forms of vitellogenin and choriogenin in red lip
mullet (Chelon haematocheilus): Immunological detection using type-specific antisera. The 6th Joint Seminar between
89
Japan and Korea by Core University Program on Fisheries Sciences –Sustainability of Fisheris in Japan and Korea-,
August 28-29, Ohnuma International Seminar House, Hakodate, Japan.
22.
Hosokawa M, Ono S, Funakoshi Y, Takahashi K, and Miyashita K (2006) Enzymatic preparation of functional marine
peptides. 97rd AOCS Annual Meeting & Expo, April 30-May 3, 2006, St. Louis, USA.
23.
Hosokawa M, Ono S, Funakoshi Y, Takahashi K, and Miyashita K (2006) Enzymatic preparation of marine peptides
and their functionalities. December 6-8, 2006, Taichung, Taiwan.
24.
Hosokawa M, Sashima T, and Miyashita K (2006) Combined anti-obesity effect of fish oil and fucoxanthin. 7th
International Conference and Exhibition on Nutraceuticals and Functional Foods, November 5-8, 2006, Reno, USA.
25.
Iimura K, Tohse H, Ura K, Takagi Y (2006) Molecular tools to study scale forming cell differentiation –cDNA cloning
and expression patterns of BMP2, Runx2 and SPARC－. 7th Korea-Japan, Japan-akorea Joint Symposium on
Aquaculture, October 2006, National Fisheries Research and Development Institute, Busan, Korea.
26.
Kasai H, Nomura T and Yoshimizu M (2006) Surveillance and Control of Salmonid Viruses of Wild Salmonid Fish
Returning to the Northern Part of Japan, from 1976 to 2005. International Symposium- How to sustain agrosphere,
biosphere and geosphere, Hokkaido University International symposium on Sustainable Development, Hokkaido
University, Japan, August 7-9, 2006.
27.
Kasai H and Yoshimizu M (2006) Creating a “Safe and Worry-Free” Salmon Products using a HACCP System from
Fishing through Processing to Distribution. Hokkaido University International Symposium on Sustainable Development
-From scientific research to practical policy recommendations-, Hokkaido University, Japan, August 6-9, 2006.
28.
Kasai H and Yoshimizu M (2006) Development of a Seawater Electrolyzer for Disease Prevention in Aquaculture and
Food Sanitation. Joint Workshop for Research Cooperation Seafood Safety between Japan and Norway, Yokohama,
Japan, November 6-7, 2006.
29.
Kasai H, Yoshimizu M and Namba K (2006) Elimination of Escherichia coli from oysters using electrolyzed seawater.
The JSPS-NRCT International Symposium Joint Seminar 2006 Innovative Technology for the Sustained Development
of Fishery and Aquaculture, Bangkok, Thailand, December 18-20, 2006.
30.
Kim SG, Morishima K, and Arai K (2006) Cross-species amplification of microsatellite markers for the brown sole in
the family Pleuronectidae. 2006 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture, October 2006, National
Fisheries Research and Development Institute, Korea.
31.
Kim Y, Kitaura H, Iguchi-Ariga SMM and Ariga H (2006) Stimulation of the Akt signal after suppression of PTEN
activity by oxidized DJ-1.Molocular Biology Forum，Dec. 2006，Nagoya．
32.
Kim YM, Funane K, Kobayashi M, Kimura A (2006) Molecular analysis of novel dextranase catalyzing endo-hydrolysis
and cyclic sugar-formation. "Korea-China-Japan Food Science Symposium" in 2006 Annual Meeting of Korean Society of
Food Science and Technology, June 14-16 2006, International Convention Center (ICC), Jeju, Korea. （Invited）
33.
Kim YM, Kimura A (2006) Novel function of α-glucosidase and its application: α-Glucosidase from Aspergillus niger,
an organic solvent-resistant enzyme, catalyzes efficient syntheses of alkyl α-2-deoxyglucoside from D-glucal and alkyl
alcohol. 2006 CAB Agricultural Biotechnology Symposium on Emerging Technology on Carbohydrate Enzymes, June
12 2006, Seoul National University, Seoul, Korea. （Invited）
34.
Kim YM, Okuyama M, Mori H, Nakai H, Saburi W, Chiba S, Kimura A (2006) Enzymatic synthesis of
α-2-deoxyglucoside derivatives by alkyl alcohols resistant α-glucosidase from Aspergillus niger. 23rd International
Carbohydrate Symposium, 2006 July 23-28, Whistler Conference Center, Whistler, Canada.
35.
Kimura Y, Yoshida T, Taira Takahiro, Iguchi-Ariga SMM and Ariga H (2006) MM-1 negatively regulates c-Myc
function by multiple pathways, including transcriptional repression, stimulation of degradation and suppression of
Wnt-signal. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress,
June 2006, Kyoto, Japan.
36.
Koide-Yoshida S, Taira T, Iguchi-Ariga SMM and Ariga H (2006) Functional analysis of DJ-1 in Familial amyloidotic
polyneuropathy. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB
Congress, June 2006, Kyoto, Japan.
37.
Kokawa Y, Takami I, Nishizawa T, and Yoshimizu M (2006) Kuchijiro-sho associated proteins (KAPs) in brain tissues
of tiger puffer and yellowtail. First International Symposium on Viral Nervous Necrosis of Fish, Hiroshima
International Convention Center, November 28-30, 2006.
38.
Lahrech Z, Morishima K, Kishioka C, Mori T, Saito S, and Arai K (2006) Microsatellite-centromere mapping and
90
verification of meiotic and mitotic gynogenetic diploids in barfin flounder, Verasper moseri. International Symposium
Genetics in Aquaculture IX, June 2006, Monpellier, France.
39.
Li Ya-Juan, Wang JB, Yuan X, Zhang W, Sun X-W, and Arai K (2006) Studies of distribution of polyploid loaches in
China. The 5th International Symposium, Industrial-Academia-Governmental Collaboration for the Establishment of
Marine Food Production in Japan and China, July 2006, Hokkaido University, Hakodate, Japan.
40.
Miyashita K (2006) Multi-functional activities of seaweed carotenoid, fucoxanthin. Korea-China-Japan International
Symposium: Asian Summit for World Foods, June 14-16, 2006, Juju, Korea. （Invited）
41.
Miyashita K (2006) Polyunsaturated lipid oxidation in aqueous systems. 97rd AOCS Annual Meeting & Expo, April
30-May 3, 2006, St. Louis, USA.
42.
Miyashita K (2006) Synergistic effects of antioxidants with other bioactives-The latest story to tell (Invited lecture). 7th
International Conference and Exhibition on Nutraceuticals and Functional Foods, November 5-8, 2006, Reno, USA.
（Invited）
43.
Miyashita K, and Alasalvar C (2006) Antioxidant and anti-obesity effects of hazelnut and olive oils polyphenols. 2nd
International Congrtess on Functional Foods and Nutraceuticals (Plenary lecture), May 4-6, 2006, Istanbul, Turky.
（Invited）
44.
Miyashita K, Hosokawa M, and Sashima T (2006) Fucoxanthin, from edible seaweeds, and its multi-biological functions. “232nd
American Chemical Society National Meeting & Exposition, September 10-14, 2006, San Francisco, USA. （Invited）
45.
Miyashita K, Hosokawa M, and Sashima T (2006) Multi-biological functionalities of seaweed carotenoid, fucoxanthin,
and the effective method for its separation. December 6-8, 2006, Taichung, Taiwan.
46.
Miyashita K, Shima Y, and Ide S (2006) Effect of droplet size and emulsifier on polyunsaturated lipid oxidation in
emulsion systems. 97rd AOCS Annual Meeting & Expo, April 30-May 3, 2006, St. Louis, USA.
47.
Mochizuki M, Amano H, Fujita T, Hiramatsu N, Hara A (2006) Purification of C-type vitellogenin (VgC) in Sakhaline
taimen. The 6th Joint Seminar between Japan and Korea by Core University Program on Fisheries Sciences
–Sustainability of Fisheris in Japan and Korea-, August 28-29, Ohnuma International Seminar House, Hakodate, Japan.
48.
Morishima K, Arias-Rodriguez L, Nakayama I, and Arai K (2006) A linkage map of the loach Misgurnus anguillicaudatus
(Teleostei:Cobitidae). International Symposium Genetics in Aquaculture IX, June 2006, Monpellier, France.
49.
Morishima K, Fujimoto T, Yoshikawa H, and Arai K (2006) Recent progress in reproductive biology and genetics of
Misgurnus loaches. 3rd International Conference Loaches of the Genus Cobitis and Related Genera, September 2006,
Sibenik, Croatia.
50.
Morishima K, Oshima K, and Arai K (2006) Hybridogenesis-like oogenesis in triploid females derived from the clone
lineage of Misgurnus loach. International Symposium Genetics in Aquaculture IX, June 2006, Monpellier, France.
51.
Moriya S, Sato S, Azumaya T, Suzuki O, Urawa S, Urano A and Abe S (2006) Genetic stock identification of chum
salmon in the Bering Sea and North Pacific Ocean using mitochondrial DNA microarray. International Symposium on
Genetics in Aquaculture IX, IAGA, June 27, 2006, Montpellier, France.
52.
Nagasato C and Motomura T (2006) Ultrastructural study on the development of Fucus zygotes. International symposium,
Marine Algae & Global Warming, October 2006, Conference Building in the National Assembly, Seoul, Korea
53.
Nakai H, Iizuka T, Okuyama M, Mori H, Chiba S, Kimura A (2006) Ion-dependent α-glucosidase from lgament and
digestive caecum of scallop. 23rd International Carbohydrate Symposium, July 23-28 2006, Whistler Conference
Center, Whistler Conference Center, Whistler, Canada.
54.
Nakai H, Iizuka T, Okuyama M, Mori H, Chiba S, Kimura A (2006) Novel ion-dependent α-glucosidase: Comparison
of α-glucosidases from ligament and digestive caecum of scallop. The 5th International Symposium on
"Industrial-Academia-Governmental Collaboration for the Establishment of Marine Production in China and Japan" by
21st Century COE program "Marine Bio-Manipulation Frontier for Food Production Toward Advanced and Safe Use of
Aquatic Organisms", July 20-22 2006, Hakodate (Japan).
55.
Nomura K, Takeda Y, Morishima K, Tanaka H, Unuma T, Arai K, and Ohta H (2006) Spontanesous polyploids and
mosaics in the progeny from artificially induced gametes of the Japanese eel, Anguilla japonica, International
Symposium Genetics in Aquaculture IX, June 2006, Monpellier, France.
56.
Oda A, Nakayama A, Okawa K, Miyazaki H, Urushibara N, Sasaki T, Nishitani C, Ishino M, Kobayashi N, Nose
K, Sasaki T, Wada I, Shaw AS, Randazzo PA, Fujita H (2006) FAK/CAKβ LINKS ASAP TO PAXILLIN. The 20 th
IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Kyoto
91
Japan，June 2006
57.
Ogawa N, Tohse H, Ura K, Shimizu M, Takagi Y (2006) Discovery of matrix substance involved in hydroxyapatite
formation from regenerating fish scales. Gordon Research Conference on Biomineralization, July 2006, Colby-Sawyer
College, New London, NH, USA.
58.
Ojima T, Inoue A (2006) Characterization and utilization of polysaccharide-degrading enzymes from marine mollusks.
Udayana Univ. & Hokkaido Univ. Workshop of Marine Biology, September 2006, Udayana University, Indonesia.
59.
Ojima T, Inoue A, Tsuchikawa M, Kumagai Y, and Katoh F (2006) Utilization of scallop viscera as a source of
glycoside-hydrolyzing enzymes. The 6th joint seminar between Japan and Korea by Core University Proguram on
Fisheries Sciences –Sustainability of Fisheries in Japan and Korea-, August 2006, Ohnuma International Seminar
House, Hakodate, Japan.
60.
Okumura S, Senbokuya H, Arai K, Sakai M, Furukawa S, and Yamamori K (2006) The characterization of survival,
growth, and male gametes in the triploid pacific abalone Haliotis discus hannai. International Symposium Genetics in
Aquaculture IX, June 2006, Monpellier, France.
61.
Rahman MdM (2006) Bovine lactoferrin binds with bifidobacteria surface proteins. 2nd Lactoferrin Forum, Nov., 2006,
Tokyo. Rahman MdM, Kim W-S, Tanaka T, Kumura H and Shimzaki K (2006) Autoaggregation ability and surface
hydrophobicity of bifidobacteria and in vitro effect of bovine lactoferrin.Meeting of Japanese Society of Lactic Acid
Bacteria，July 2006，Oosaka．
62.
Sachindra NM, Hosokawa M, and Miyashita K (2006) Antioxidative properties of extracts from Indian seaweeds. 45th
Meeting of Japan Oil Chemist’s Society, Sept. 2006．
63.
Saito T, Goto-Kazeto R, Arai K, and Yamaha E (2006) Germ-line replacement between different species by
transplantation of a single promordial germ cell, 7th International Conference on Zebrafish Development and Genetics,
June 2006, University of Wisconsin-Madison, USA.
64.
Sasaki T, Oda A, Nakayama A, Okawa K, Miyazaki H, Urushibara N, Nose K, Fujita H (2006) β PIX Bridges Nck and
GIT1. 20 th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress,
Kyoto Japan, June 2006.
65.
Shimizu T, Kasai H and Yoshimizu M (2006) Biological Control of Viral Diseases in Larvae Using Anti-viral
Substance-Producing Bacteria. 2nd International Biomicrocosmos Workshop -For the Enhancement of Gastro-Intestinal
Biosphere Research-. Hokkaido University, Japan, February 2-3, 2006.
66.
Shimizu T, Kasai H and Yoshimizu M (2006) Manipulating diets with anti-viral substance-producing bacteria for seed
production of marine fish. The 5th International Symposium “Industrial-Academia-Governmental Collaboration for the
Establishment of Marine Food Production in China and Japan”, Hakodate, Japan, July 20-22, 2006.
67.
Shin D-H, Omoto N, Arai K, Adachi S, Yamauchi K (2006) Artificial propagation in sturgeon. The 6th Joint Seminar
between Japan and Korea by Core Univ.Program on Fisheries Sciences-Sustainability of Fisheries in Japan and Korea,
August 2006, Ohnuma International Seminar House, Nanae, Japan.
68.
Shin D-H, Omoto N, Wu Q, Yamaha E, Arai K, Adachi S, and Yamauchi K (2006) Artificial control of reproduction for
aquaculture and conservation in sturgeon. 2006 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture, October
2006, National Fisheries Research and Development Institute, Korea.
69.
Soyano K, Aoki J, Lee YD, Nagae M, Hara A (2006) Survey of estrogenic contaminations in coastal area of East China
Sea. (abstract pp. 12-13), Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of Aquatic Animals,
November 20-21, Cheju National University, Cheju, Korea.
70.
Sullivan CV, Williams VM, Reading BJ, Hiramatsu N, Sawaguchi S, Matsubara T, Amano H, Hara A (2006) The
multiple vitellogenin system of Xenotoca eiseni, a moderately matritrophyc goodeid. The 3rd International Symposium
on Viviparous Fishes, November 8-11, Morelia, Michoaca, Mexico.
71.
Taira T, Inden M, Kitamura Y, Miyazaki M, Iguchi-Ariga SMM and Ariga H (2006) Function of DJ-1, a causative gene
product for familial Parkinson’s disease, and its therapeutic application to Parkinson’s disease. 20th IUBMB
International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, June 2006, Kyoto, Japan.
72.
Takagi Y (2006) Hard tissue research and application in aquatic animals. The 6th International Symposium,
“Symposium on Development of Fisheries Science in Asia”, 21st Century COE Program, Marine Bio-Manipulation,
Frontier for Food Production, December 2006, Shanghai Fisheries University, Shanghai, People’s Republic of China.
73.
Takagi Y, Tohse H, Ura K (2006) Potential application of fish scale collagen in tissue engineering. 7th Korea-Japan, Japan-akorea
92
Joint Symposium on Aquaculture, October 2006, National Fisheries Research and Development Institute, Busan, Korea.
74.
Tohse H, Takagi Y, Nagasawa H (2006) Otolican-64, a novel matrix protein from fish otolith and its possible function
on development and biomineralization of otoliths. Gordon Research Conference on Biomineralization, July 2006,
Colby-Sawyer College, New London, NH, USA.
75.
Wongchawalit J, Yamamoto T, Okuyama M, Mori H, Surarit R, Svasti J, Chiba S, Kimura A (2006) α-Glucosidase
isozymes from Asian honeybees to exhibit allosteric kinetics. 20th IUBMB International Congress of Biochemistry and
Molecular Biology, 11th FAOBMB, 79th Annual Meeting of the Japanese Biochemical Society and 29th Annual
Meeting of Molecular Biology Society of Japanese, June 18-23 2006, Kyoto International Conference Hall, Kyoto.
76.
Yasmin A, and K Miyashita (2006) Dietary effect of pomegranate seed oil on lipid and fatty acid composition in masu
salmon Oncorhynchus masou, Annual Meeting of Japan Society of Fisheries Science，March 2006．Kouchi.
77.
Yoon M, Sato S, Seeb JE, Brykov V, Seeb LW, Varnavskaya N, Wilmot RL, Urawa S, Urano A, and Abe S (2006)
Congruence of population genetic profiles obtained from mitochondrial and microsatellite DNA analyses in the Pacific
Rim chum salmon. The 2nd NPAFC International Workshop on Factors Affecting Production of Juvenile Salmon,
Conference Hall of Hokkaido Governmental Building, North Pacific Anadromous Fish Commission, April 26, 2006,
Sapporo, Japan.
78.
Yoon M, Sato S, Urawa S, Urano A, and Abe S (2006) Genetic variation and population structure of chum salmon
inferred from the mitochondrial and microsatellite DNAanalyses, Annual Meeting of Japan Society of Fisheries Science，
March 2006．Kouchi.
79.
Yoshikawa H, Morishima K, Fujimoto T, and Arai K (2006) Reproductive capacity of diploid-triploid mosaic females
in the loach. The 5th International Symposium, Industrial-Academia-Governmental Collaboration for the Establishment
of Marine Food Production in Japan and China, July 2006, Hokkaido University, Hakodate, Japan.
80.
fishing through processing to distribution. The 73rd Annual Meeting of Korean Society of Food Science & Technology,
(Korea-China-Japan International Symposium Asian Summit for World Foods,) Jeju ICC, Korea, June 14-16, 2006.
81.
Yoshimizu M and Kasai H (2006) Creating a safe and worry-free salmon products using a HACCP system from fishing
through processing to distribution – the Shibetsu Town District HACCP Program model–. The 5th International
Symposium “Industrial-Academia-Governmental Collaboration for the Establishment of Marine Food Production in
China and Japan”, Hakodate, Japan, July 20-22, 2006.
82.
Yoshimizu M and Kasai H (2006) Creating Safe and Worry-Free Salmon Products using a HACCP System. Joint
Workshop for Research Cooperation Seafood Safety between Japan and Norway, Yokohama, Japan, November 6-7, 2006.
83.
Yoshimizu M, Furihata M, Kasai H and Nishizawa T (2006) Re-Immerging OMV-Disease of Rainbow Trout and its
Control Strategy. 5th International Symposium on Aquatic Animal Health, San Francisco, USA, September 2-6, 2006.
84.
Yoshimizu M, Kasai H and Nishizawa T (2006) Oncorhynchus masou virus disease: Re-immerging OMVD of rainbow
trout and its control strategy. OIE Grobal Conference on Aquatic Animal Health, Bergen, October 9-12, 2006.
85.
Yoshimizu M, Kim WS, Kasai H and Nishizawa T (2006) Evaluation of Methods for Sero-Epidemiology and
Surveillance of Infectious Hematopoietic Necrosis. The 11th Conferences of International Society for Veterinary
Epidemiology and Economics, Cairns, Australia, August 6-11, 2006.
86.
Yoshimizu M, Shimizu T, Yoshida N and Kasai H (2006) Evaluation of Survival of Koi Herpesvirus in Environmental
Water. 5th International Symposium on Aquatic Animal Health, San Francisco, USA, September 2-6, 2006.
Other 169 presentations indomestic meetings.
93
Appendix 5
International COE Symposia
The COE program held International COE Symposia to discuss current trends of science in
the specific fields.
2005 February
Six symposia have been held.
1st International COE Symposium “Potential and Perspective of Marine
Bio-Manipulation”
2005 May
page 95
2nd International COE Symposium “Marine resources as Functional and
Therapeutic Agents”
2005 July
Sapporo, Japan, 120 Attendees
Sapporo, Japan, 145 Attendees
page 97
3rd International COE Symposium “Industrial-Academia-Governmental
Collaboration for the Establishment of Marine food Production in China
and Japan” Shanghai, China, 110 Attendees
2005 October
4th International COE Symposium “Reproductive, Genetic and Disease
Management in Aquaculture and Ocean Ranching”
Attendees
2006 July
page 99
Hakodate, Japan, 200
page 101
5th International COE Symposium “Industrial-Academia-Governmental
Collaboration for the Establishment of Marine food Production in China
and Japan ”
2006 December
Hakodate, Japan, 120 Attendees
page 105
6th International COE Symposium “Symposium on Developing Fisheries
Science in Asia” Shanghai, China, 40 Attendees
94
page 107
1st International COE Symposium
“Potential and Perspective of Marine Bio-Manipulation”
Saturday 26 February 2005
(ENYUU GAKUSHA)
12:00 - 17:00
Registration
13:30 - 13:35
Opening Remark : YAMAUCHI, Kohei
（Leader of the 21COE Program）
13:35 - 13:45
Scope of the Symposium: ARAI, Katsutoshi
（Vice- Leader of the 21COE Program）
13:45 - 15:30
Poster Session
15:30 - 17:00
Welcome Reception
Sunday 27 February 2005
(CONFERENCE HALL)
9:30 - 17:00
Registration
Chair: NAKAYAMA, Ichiro (National Research Institute of Fisheries Science/JAPAN)
10:00 - 10:40 THORGAARD, Gary H.
From Genetic Manipulation to Genomic Analyses
10:40 - 11:20 BENZIE, John A. H.
Marine Shrimp Genomics
11:20 - 12:00 WAKAMATSU, Yuko
The Present Status of Fish Cloning by Nuclear Transplantation:The Course of Our Ten
Years
95
12:00 - 13:30
Lunch Time
Chair: WADA, Katsuhiko T. (Japanese Society of Fish Genetics and Breeding Science/JAPAN)
13:30 - 14:10 GONG, Zhiyuan (National University of Singapore/SINGAPORE)
Applications of Transgenic Fish Technology
14:10 - 14:50 NAGAHAMA, Yoshitaka
Sex Determination and Differentiation in Fish
14:50 - 15:20
Coffee Break
Chair: ABE, Syuiti (Hokkaido University/JAPAN)
15:20 - 16:00 CLOUD, Joseph G.
Gonad Culture, Cryopreservation and Transplantation: Tools to Support BioManipulation
16:00 - 16:40 YAMAHA, Etsuro
Bio-Manipulation of Fish Embryos Toward Surrogate Production in Aquaculture
16:40 - 17:00
Concluding Remarks :
ARAI, Katsutoshi
SULLIVAN, Craig V. (North Carolina State University/USA)
96
2nd International COE Symposium
“Marine resources as Functional and Therapeutic Agents”
Wednesday May 25 2005
(ENYUU GAKUSHA)
12:00 - 17:00
Registration
13:30 - 13:45
Scope of the Symposium: Miyashita K
(Symposium Convener)
13:45 - 15:30
Poster Session
15:30 - 17:00
Mixer
Thursday May 26 2005
(CONFERENCE HALL)
9:30 - 16:40
Registration
Chair: Kimura A (Hokkaido University/Japan)
10:00 - 10:50 Yu S
The Discovery of New enzymes, New products and a New Pathway from Marine Red
Algae
10:50 - 11:40 Abe J
Preparation of 1,5-anhydro-D-fructose using enzyme red algae, and its contribution to
food safety as antimicrobial agent
11:40 - 12:30 Kawano K
Solution structure and function of antimicrobial peptides isolated from hemocytes of
horseshoe crab
12:30 - 13:30
Lunch Time
Chair: Shimazaki K (Hokkaido University/Japan)
13:30 - 14:20
Dong Z
97
Cancer Prevention: Resources from Ocean
14:20 - 15:10 Sato K
Biological effect of fish extracellular matrix components
15:10 - 15:30
Coffee Break
15:30 - 16:20 Morrissey M T
Production of n-3 Polyunsaturated Concentrate from Sardine Oil by Lipase-catalyzed
Hydrolysis
16:20 - 16:40
Concluding Remarks :
Miyashita K
98
3rd International COE Symposium
“Industrial-Academia-Governmental Collaboration for the Establishment of Marine
Food Production in China and Japan”
11th July
Academic Collaboration Agreement: At Garden hotel Shanghai
9:30 - 9:35
Opening remarks: Chair: Huang Shuo-Lin (Shanghai Fisheries University,
Vice-president, Professor)
9:35 - 9:45
Pan Ying-Jie (Shanghai Fisheries University, Professor)
9:45 - 9:55
Kohei Yamauchi (Hokkaido University, Faculty of Fisheries, Dean,
Professor)
Program for industry-academic-government (morning session): At Garden hotel
Shanghai
10:30 - 10:45
Wang Qi (Shanghai City Education Committee, Vice-chairman)
10:45 - 11:00
Masanori Nishio (Hakodate City office, Deputy mayor)
11:00 - 11:15
Shanghai city Scientific committee, Vice-chairman
11:15 - 11:23
Yataro Numasaki (Chamber of Commerce and Industry of Hakodate,
Vice-chairman)
11:23 - 11:30
Toshio Suzuki (Suruga Seiki Co., LTD. CEO)
Program for industry-academic-government (afternoon session): At Shanghai Fisheries
University
14:00 - 14:30
Pan Ying-Jie （Shanghai Fisheries University, Professor）
14:30 - 15:00
Kohei Yamauchi (Hokkaido University, Faculty of Fisheries, Dean,
Professor)
15:00 - 15:30
Gu Dao-Liang (Shanghai City, Fisheries Department, Department Director)
16:00 - 16:30
Hideo Kou (Hakodate City, Planning Division, Counselor)
16:30 - 16:50
Kong Qing-Yuan (Shanghai City, Fisheries Association)
16:00 - 17:10
Hiromichi Kaneko (Marukichi Foods Co., LTD., Executive Director)
17:10 - 17:30
Ye Hui-De (Ronhou Food Company, President)
12th July
Scientific Program: At Shanghai Fisheries University
Chair: Yasuaki Takagi (Hokkaido University, Faculty of Fisheries, Professor)
Xie Jing (Shanghai Fisheries University, Professor）
99
9:30 - 10:00
10:00 - 10:30
Li Si-Fa (Shanghai Fisheries University, Chief Professor）
Aquaculture development in China
Katsutoshi Arai (Hokkaido University, Faculty of Fisheries, Professor, 21st
COE Program Sub-leader)
The Initiative in Applied Life Science for Aquaculture and Marine
Bio-industry
1030 - 11:00
Xu Liu-Xiong (Shanghai Fisheries University, Professor）
Review on the development of China’s Deep sea fisheries
11:00 - 11:30
Kohji Iida (Hokkaido University, Faculty of Fisheries, Professor)
Introduction of the Research Field of Marine Environment and
Resources in Hokkaido University
Chairman:
Gao Jian (Shanghai Fisheries University, Professor）
Koretaro Takahashi (Hokkaido University, Faculty of Fisheries, Professor)
13:00 - 13:30
Xie Jing (Shanghai Fisheries University, Professor）
A Glance at the College of Food Science & Technology in SHFU,
Aquatic Food Industry in China
13:30 - 14:00
Kunihiko Konno (Hokkaido University, Faculty of Fisheries, Professor)
Highly and full utilization of marine bioresources
14:00 - 14:30
Xie Jing-Hua (Shanghai Fisheries University, Assistant Professor）
Import and Export of Salmon in China
15:00 - 17:00
13th July
9:00 - 12:00
Poster Presentation
Group Meeting: Shanghai Fisheries University
100
4th International COE Symposium
“Reproductive, Genetic and Disease Management in Aquaculture and Ocean Ranching”
October 8, Saturday (Day 1)
17:00-20:00 Registration & Welcome Reception
October 9, Sunday (Day2)
Session I: Fish Reproduction – Molecular Mechanism of Gametogenesis
09:00-09:05 Opening Address for the Symposium: Yamauchi K
09:05-09:10 Opening Remarks: Adachi S
Chair: Dickhoff W W
09:10-09:45 Nakamura M
Sex Change in Fish
09:45-10:20 Swanson P, Campbell B
Interactions Between Growth and Reproduction in Salmonid Fish: Potential
Endocrine Mechanisms
10:20-10:35 Tea Break
Chair: Matsuyama M
10:35-11:10 Lokman P M, Algie M, Matsubara H
Effects of Androgens and Growth Factors During Early Oogenesis in Fish
11:10-11:45 Young G, Nakamura I, Kusakabe M
Gonadal steroidogenic protein genes in teleosts: seasonal changes in
expression and endocrine control
11:45-13:00 Lunch
Chair: Ariga S
13:00-13:35 Special Lecture I: Svensson B
Opportunities, Challenges and Responsibilities of Women in Biochemistry in
Europe
13:35-13:45 Tea Break
Chair: Ueda H
13:45-14:20 Yamashita M
The Formation, Activation and Function of Maturation-Promoting Factor
(MPF), the Final Inducer of Oocyte Maturation
14:20-14:55 Scott A P, Li W, Zielinski B, Corkum L D
Strategies for Identifying Fish Pheromones
14:55-15:30 Miura T
101
The Analysis of Fish Gametogenesis Using In Vitro Culture System
15:30-16:00 Concluding Remarks: Adachi S
16:00-18:00 Poster Session I
(16:00-16:45 Presenters for odd-numbered posters, 16:45-17:30 Presenters for
even-numbered posters)
October 10, Monday (Day3)
Session II: Aquaculture Genetics – from Genome to Resource Management
09:00-09:05 Opening Remarks: Abe S
Chair: Nakayama I
09:05-09:50 Nishida M, Miya M
Molecular phylogeny of ray-finned fishes: a whole mitochondrial genome
approach
09:50-10:35 Phillips R B
Salmon Genome Analysis
10:35-10:45 Tea Break
Chair: Arai K
10:45-11:30 Gharrett A J
Genetics in Fisheries Science
11:30-12:15 Taniguchi N and Ortega-Vilaizan Romo M M
Broodstock Management of Endangered Fish in Resource Enhancement
Program
12:15-13:15 Lunch
Chair: Soyano K
13:15-14:15 Special Lecture II: Sawada Y, Kumai H
Early Development and Fingering Production of the Pacific Bluefin
Tuna, Thunnus orientalis
14:15-14:25 Tea Break
Chair: Urawa S
14:25-15:10 Sato M,, Nagashima K, Kawamata K, Zaslavskaya N, Nakamura A, Ohta T,
Nishikiori T, Tanaka A, Yoshikawa S, Brykov V
Molecular population genetic study of Japanese scallop
15:10-15:55 Seeb J E, Abe S, Smith C T, Templin W D, Seeb L W
Molecular population genetics and management of Pacific salmon:
developing single nucleotide polymorphism (SNP) assays to define geographic
structure
102
15:55-16:00
Closing Remarks: Abe S
16:00-18:00 Poster Session II
(16:00-16:45 Presenters for odd-numbered posters, 16:45-17:30 Presenters for
even-numbered posters)
18:00-20:00
Banquet
October 11, Tuesday (Day4)
Session III: Seed Production and Disease Management in Aquaculture and Ocean Ranching
09:00-09:05 Opening Remarks: Yoshimizu M
Chair: Kagawa H
09:05-09:45 Special Lecture III: Tanaka H
Advances in Techniques for Induction of Maturation, Artificial
Fertilization, and Larviculture in Japanese Eel
09:45-10:00 Tea Break
Chair: Hara A
10:00-10:40 Sullivan C V, Couch C R, Garber A F, Hiramatsu N, Hiramatsu K,
Rexroad III C, Westerman M E
Hybrid Striped Bass Farming in the United States: Research and
Industry Development
10:40-11:20 Li S-F
The Present Status and the Challenges of Aquaculture in China
11:20-12:00 Kato K, Ishimaru K, Mutsuro J, Kato F, Murata O, Kumai H
The Necessity of Disease Management in the Seedling Production of
the Marine Finfish
12:00-13:00 Lunch
Chair: Suzuki S
13:00-14:00
Special Lecture IV: Leong J-A
Current Status of Vaccine development for fish viral diseases
Chair: Oh M-J
14:00-14:40 Olesen, N J
Advances in surveillance and control of viral diseases in rainbow trout
14:40-15:20 Ruangpan L
Disease Problems and Control of Shrimp Culture in Thailand
15:20-15:40 Tea Break
Chair: Ruangpan L
103
15:40-16:20
Oh M-J, Nishizawa T, Kitamura S-I, Jung S-J, Yoshimizu M
Study of Lymphocystis Disease (LCD) and Lymphocystis Disease
Virus (LCDV)
16:20-17:00 Suzuki S, Inaba M, Kitamura S, Kumazawa N H, Kodama H
Marine Birnavirus (MABV): Strategies of Wide Distribution in Various
Hosts and Environments
17:00-17:30
Concluding Remarks: Yoshimizu M
104
“Industrial-Academia-Governmental Collaboration for the Establishment of Marine
Production in China and Japan”
21th July: At Hokkaido University, Hakodate
08:45 - 09:00 Opening Remarks
Chairman: Hiroki Saeki (Hokkaido University),
Wang Xi-Chang (Shanghai Fisheries University)
09:00 - 09:30
Koretaro Takahashi (Hokkaido University, Faculty of Fisheries, Professor)
Conversion of fisheries by-products into value added products
09:30 - 10:00
Chen Shun-Sheng (Shanghai Fisheries University, Professor）
Taste-active compounds in Chinese mitten-handed crabs
10:00 - 10:30
Kazuo Miyashita (Hokkaido University, Faculty of Fisheries, Professor)
10:30 - 11:00
Liu Cheng-Chu (Shanghai Fisheries University, Professor）
Vibrio parahaemolyticus: occurrence in oysters in Shanghai fisheries
markets, development of a rapid method for detection and study of
inactivation by wine consumption
11:00 - 11:30 Kouji Yamazaki (Hokkaido University, Faculty of Fisheries, Associate
Professor)
Control of food-borne pathogens using biopreservatives of microbial
origin in food
11:30 - 12:00
Wu Wen-Hui (Shanghai Fisheries University, Professor）
Enhancement of plasminogen activation by a low molecular compound
from the metabolite of fungi
12:00 - 14:00 Poster Presentation
Chair:
Takao Ojima (Hokkaido University), Li Bo-Lin (Shanghai Fisheries Universit)
14:00 - 14:30
Yuan Chun-Hong (Hokkaido University, Faculty of Fisheries, Assistant
Professor)
Functionality of muscle proteins of several Chinese freshwater fish as
food
14:30 - 15:00
Tao Yan (Shanghai Fisheries University, Professor）
Temperature-dependent expression patterns of grass carp fast skeletal
myosin heavy chain genes
105
15:00 - 15:30
Hirotoshi Endo (Hokkaido University, Faculty of Fisheries, Assistant
Professor)
Porphyra yezoensis: A model plant in marine biosciences
15:30 - 16:00
Yan Xing-Hong (Shanghai Fisheries University, Professor）
Genetic studies on Porphyra haitanensis (Rhodophyta)
Chairman: Yasuaki Takagi (Hokkaido University), Zhong Junsheng (Shanghai Fisheries
University)
16:15 - 16:45
Shinji Adachi (Hokkaido University, Faculty of Fisheries, Professor)
Artificial control of reproduction in sturgeon
16:45 - 17:15
Zhang Sou-Yu (Shanghai Fisheries University, Professor）
Seasonal variation of fishery resources in Gouqi Island Seaweeds
17:15 - 17:30
Closing Remarks
106
“Symposium on Developing Fisheries Science in Asia”
December 18, Monday
9:00 – 9:05
Welcome Address: Xiang-jun Leng
9:05 – 9:15
Opening Remarks: Shuo-lin Huang
9:15 – 9:25
Opening Remarks: Shinji Adachi
Symposium I:
9:25 – 9:55
Jia-le Li: Introduction for College of Aqua-life Science and Technology,
SFU
9:50 – 10:20
Katsutoshi Arai: Research Activities for Advanced Aquaculture Studies in
the Division of Marine Life Science
Symposium II:
10:30 – 11:00
Si-fa Li: Introduction of Laboratory of Genetic Resources of Aquatic
Animals (LGRAA), SFU
11:00 – 11:30
Yasuaki Takagi: Hard Tissue Research and Application in Aquatic Animals
11:30 – 12:00
Yong-xu Cheng: The scientific researches in animal aquaculture at
Shanghai Fisheries University
13:30 – 14:00
Syuiti Abe: Aquagenomics and molecular population genetics of marine
animals
14:00 – 14:30
Min Jiang: Brief Introduction of Aqueous Environment Laboratory
14:30 – 15:00
Naoshi Hiramatsu: Development of Monitoring System on Endocrine
Disruption
Symposium III:
15:30 – 15:55
Pei-min He: Seaweed bioremediation in marine eutrophication and their
bioactive substance utilization
15:55 – 16:20
Hideshi Seki: Separation Technology for Environmental Conservation and
Remediation
16:20 – 16:45
Bao-long Bao: A new model for explaining asymmetrical eye migration in
107
Japanese flounder Paralichthys olivaceus
16:45 – 17:10
Munetaka Shimizu: A physiological approach to estimate year-class
strength of salmon stocks
17:10 – 17:35
Jing-liang Zhao: Genetic diversities of sinipercine fishes in China
17:35 – 18:00
Shigeho Ijiri: Sex differentiation of Tilapia gonad
December 19, Tuesday
9:00 – 10:30
Whole Discussion
10:30 – 12:00
Group Discussion
13:30 – 17:30
Visiting Aquaculture Farm
108
Appendix 6
International Cooperation
Faculty of Fisheries Sciences, Hokkaido University (HU) has concluded 21 academic
exchange agreements with foreign universities and public institutions. Among them 5
agreements, the agreements with Shanghai Fisheries University, Ocean University of China,
Udayana University, South East Asian Fisheries Development Center and Kasetsart University,
are actively supported by the COE program. In some other exchange programs, members of
the COE program are actively participating.
Shanghai Fisheries University (SFU), Shanghai, Chaina
page 110
Ocean University of China (OUC), Qingdao, China
page 112
Udayana University (UU), Bali, Indonesia
page 113
Southeast Asia Fisheries Development Center (SEAFDEC), Bangkok, Thailand
page 114
Faculty of Fisheries, Kasetsart University (KU), Bangkok, Thailand
page 116
Dalian Fisheries University (DFU), Dalian, China
page 117
Kangnung National University (KNU), Kangnung, Korea
page 119
College of Ocean Science, Cheju National University (CNU), Cheju, Korea
page 120
College of Fisheries, Chonnam National University (CNU), Yosu, Korea
page 121
Warmia and Mazury University (WMU), Olsztyn, Poland
page 122
Department of Zoology, North Carolina State University (NCSU), Raleigh, NC, USA
page 123
109
Cooperative Exchange with Shanghai Fisheries University (SFU), Shanghai, Chaina
Objectives and achievements
Objective of the cooperation is to establish fruitful system of industrial-academiagovernmental collaboration for the marine food production in China and Japan. Four
symposia have been held for promotion of mutual understandings. The Academic Exchange
Agreement was concluded and satellite offices of both universities were established. In
addition to short term exchanges of researchers, log term stay (one year) of undergraduate and
graduate students from Shanghai Fisheries University in Hakodate has been conducted.
Number of people exchanged was as follows.
Year
2004
2005
2006
To SFU
8
36
20
Researchers
To HU
0
6
17
To SFU
0
0
1
Students
To HU
2
2
2
Brief history of exchange
2004 December
1st meeting for cooperation in Shanghai
Possible academic collaboration was discussed and future conclusion of
Academic Exchange Agreement was agreed.
2005 June
2nd meeting for cooperation in Hakodate
2005 July
1st symposium for industrial, academic and governmental exchange in
Shanghai (3rd International COE-Symposium “Industrial-AcademiaGovernmental Collaboration for the Establishment of Marine Food
Production in China and Japan”)
At the beginning of the symposium, The Academic Exchange Agreement
was concluded, and Shanghai office of the Faculty of Fisheries Science,
Hokkaido University was established.
cf. Appendix 5 for the symposium program.
2006 April
Dr. Chunhong YUAN who were from Shanghai Fisheries University was
employed as the Assistant Professor in the Faculty of Fisheries Sciences,
Hokkaido University, in order to promote further academic exchange
between the two universities. .
2006 July
2nd symposium for industrial, academic and governmental exchange in
Hakodate (5th International COE-Symposium “Industrial-AcademiaGovernmental Collaboration for the Establishment of Marine Food
Production in China and Japan”)
Hakodate office of Shanghai Fisheries University was established.
2006 December
3rd symposium for academic exchange in Shanghai (6th International
COE-Symposium “Developing fisheries science in Asia”)
110
After the academic exchange, practical collaborative research groups
were established. The objective of the collaborative project was set to
develop sustainable aquaculture system in the area where environmental
degradation was going on. The system should integrate aquaculture
system and biological and chemical systems for environmental restoration
technologies.
111
Cooperative Exchange with Ocean University of China (OUC), Qingdao, China
Objectives and Achievements
Objective of the cooperation is to establish fruitful relationships for corroborative research
and education. The Academic Exchange Agreement was concluded in 2005.
Year
2004
2005
2006
To OUC
0
6
15
Researchers
To HU
0
2
0
To OUC
0
0
1
Students
To HU
0
1
1
Brief History
2005 November
The Academic Exchange Agreement was concluded in Hakodate.
2005 September One graduate school student (doctoral course) entered to HU.
2006 October
International Symposium on Sustainable Use of Aquatic resources was
held in Qingdao. Fifteen HU members were attended.
112
Cooperative Exchange with Udayana University (UU), Bali, Indonesia
Objectives and Achievements
Objective of the cooperation is to support establishment of research and education system
of Fisheries and Ocean Sciences in the UU by corroborative research and education with
Hokkaido University (HU). The Academic Exchange Agreement was concluded in 2005.
Year
2004
2005
2006
Researchers
To UU
0
5
7
To HU
0
0
0
Students
To UU
0
0
0
To HU
0
0
0
Brief History
2005 September The Academic Exchange Agreement was concluded in UU, Bali, and
Memorial symposium and workshop were held.
2006 September Udayana University and Hokkaido University Workshop of Marine
Biology was held in the UU. Special Lectures for young scientists and
students were given from professors of HU.
113
Cooperative Exchange with the Southeast Asia Fisheries Development Center
(SEAFDEC), Bangkok, Thailand
The main objective of cooperative exchange with the SEAFDEC, which is an
intergovernmental regional organization, is to establish an effective regional network between
HU and the SEAFDEC member countries to promote future cooperative activities in fisheries
research, education and technology in Southeast Asia, because fisheries are
socio-economically important in Southeast Asia and Japan. As fisheries sciences are
adaptable and practical to the fisheries sector, it is essential for fisheries scientist to facilitate
close cooperation not only with other scientist, but also with fisheries agencies, which is
under the national fisheries authority. By establishing a regional network with the
SEAFDEC, HU expects to better understand the needs of fisheries research, study and
technical cooperative programs with individual SEAFDEC member countries, which is
expected to stimulate new research at HU. Through the framework of this cooperative
agreement, HU has started the exchange of students, taking advantage of various SEAFDEC
training programs.
Year
2006
2007
Researchers
To SEAFDEC
To HU
7
0
18
0
Students
To SEAFDEC
To HU
3
0
2
0
2006 February
Conclusion of Agreement for Cooperative Exchange in Bangkok, Thailand
HU mission (seven staff) visited the SEAFDEC Secretariat and Training
Department in Bangkok, Thailand.
2006 April
HU dispatched two graduate students to an international regional training
course in Bangkok organized by SEAFDEC Training Department.
2006 October
HU dispatched one undergraduate student to a Thai university
students-training course in Bangkok, organized by SEAFDEC Training
Department.
2007 February
Introductory mission (three staff) from HU to the SEAFDEC Regional
Technical Consultation held in Chiang Mai, Thailand.
At the meeting, HU briefly introduced its institutional background
including ongoing educational programs and scientific research to
SEAFDEC member countries to facilitate future cooperative projects.
2007 March
1st meeting for Scientific, Technical and Educational Exchange between the
SEAFDEC and HU
At the meeting, HU and each SEAFDEC department introduced its
ongoing programs and exchanged initial views about future cooperation in
fisheries research and education. Participants at the meeting suggested
114
that a second meeting be held to involve member countries and to develop
a framework to facilitate a stable cooperative program.
2007 August
HU dispatched one graduate student to the Training Workshop on Fisheries
Refugia organized by the SEAFDEC Training Department in collaboration
with the UNEP/GEP
2007 September HU dispatched one graduate student to the International Training Course on
Coastal Fisheries Management organized by the SEAFDEC Training
Department
115
Cooperative Exchange with the Faculty of Fisheries, Kasetsart University (KU),
Bangkok, Thailand
The objectives of cooperation between HU and KU are to strengthen fisheries research and
education between the universities and to make the relationship between universities a core
for future extension of fisheries scientific research and educational programs in the Southeast
Asia because KU is a leading university in fisheries sciences in Thailand and Southeast Asia.
Working groups at the first exchange meeting between the universities agreed that the first
step in educational exchange would be several short-term intensive lectures by HU teaching
staff to KU students at the KU campus. HU and KU are now deliberating the next step
based on an evaluation of these lectures.
Year
2007
To KU
17
Researchers
To HU
4
To KU
0
Students
To HU
0
2007 February
Conclusion of Cooperative Exchange Agreement between HU and KU
A KU mission (4 staff) led by Dr. Musig, Dean of the Faculty of
Fisheries Science at KU, visited the Hakodate campus of HU and discussed
the possibility of holding the 1st exchange meeting in Bangkok, Thailand.
2007 March
1st exchange meeting for mutual introductions in Bangkok, Thailand
At the meeting, participants from both universities presented their
research interests and the ongoing educational programs at both
universities. Participants agreed on the importance of educational
cooperation and exchange between the universities along with
internationalization at higher education levels, and established respective
working groups to review the necessary educational programs between HU
and KU.
2007 August
1st lecture exchange program at the KU campus
HU dispatched two teaching staff to KU during 19-25 August 2007 to
give special intensive lectures. According to an agreed-upon schedule,
the two staff gave 14 lectures, which were attended by a total of more than
600 students.
116
Cooperative exchange with Dalian Fisheries University (DFU), Dalian, China
Objective of the cooperation is to establish research and educational net-work for the
sustainable production and safe use of marine resources in the East Asia. Since the
conclusion of the Academic Exchange Agreement between the Faculty of Fisheries Sciences,
Hokkaido University (HU) and Dalian Fisheries University (DFU) as the first partner in
Chinese universities in September 2000, exchanges of researchers have been conducted for
special seminars, lectures and cooperational research projects. Long-term stay of Chinese
researcher (Li Ya-Juan, associate professor) in HU was realized by the program of China
Scholarship Council (CSC) in 2004 to 2005. Short-term stay of researchers from DFU and
HU has been operated by some JSPS (Japan Society for the Promotion of Science) Programs.
In addition, long term stay (one year) of undergraduate and graduate students from DFU in
HU has been conducted.
Number of peoples exchanged was as follows.
Year
2002
2003
2004
2005
2006
To DFU
1
0
1
0
3
Researchers
To HU
0
0
1
1
6
To DFU
0
0
0
0
1
Students
To HU
2
2
2
2
2
2000 September Conclusion of Academic Exchange Agreement
2002 November
Special lectures by Prof. K. Arai at DFU
2004 October
Long-term (one year) research stay of Assoc. Prof. Y.-J. Li at HU by CSC
program
2006 July
Short-term (three months) stay of Assoc. Prof. Y.-J. Li at HU for the
Ronpaku (Dissertation Ph.D) Program by JSPS
2006 September Five Professors of DFU visited HU for academic exchanges.
2006 October
Prof. K. Iida visited DFU for cooperative research by JSPS Program
2006 November
Prof. K. Arai visited DFU for special lectures and supervising the Ronpaku
Program by JSPS.
2007 July
Prof. K. Arai visited DFU for special lectures and supervising Ronpaku
Program by JSPS.
2007 July
Short-term (three months) stay of Prof. Y.-J. Li at HU for the Ronpaku
Program by JSPS
117
2007 November
The 1st Meeting of Asian Fisheries Acoustic Society will be held at DFU.
The organizing committee includes Prof. K. Iida (HU) and Prof. Y. Tang
(DFU, Former Postdoctoral fellow at HU).
118
Cooperative Exchange with Kangnung National University (KNU), Kangnung, Korea
The cooperation is aiming to establish fruitful system of industrial-academia-governmental
collaboration for the marine food production in Korea and Japan. The Academic Exchange
Agreement was concluded this year (2007) on the basis of the past 6 years exchange of
researchers and students summarized below.
Year
2001
2002
2003
2004
2005
2006
To KNU
2
3
3
0
4
0
Researchers
To HU
4
0
0
3
5
3
To KNU
0
0
0
0
0
0
Students
To HU
0
0
0
1
1
16
2001
Academic exchange was started on the basis of the Core University
Program on Fisheries Science between Japan and Korea (FisCUP)
co-sponsored by JSPS and KOSEF, and accepted four researchers from
KNU (July, August and October).
*JSPS, Japanese Society for the Promotion of Science; KOSEF, Korean
Science and Engineering Foundation
2002
Three researchers were sent to KNU for discussion of collaborative works
under the FisCUP (August and October).
2003
Two researchers were sent to and one researcher was accepted from KNU
for collaborative works and academic exchange, respectively, under the
FisCUP (October and December).
2004
Three researchers were accepted from KNU under the FisCUP (June and
July). One graduate student (doctor’s course) was accepted from KNU.
2005
Four researchers were sent to and five researchers were accepted from
KNU for collaborative works and academic exchanges under the FisCUP
and the JSPS Researchers Exchange Program (May, June, and August).
2006
One researcher was accepted from KNU for collaborative works under the
FisCUP (February). KNU sent 16 undergraduate trainee of fisheries
sciences, with three supervising researchers, under the New University for
Regional Innovation (NURI) program sponsored by Korean government
(October). At that occasion, preliminary discussion for Academic
Exchange Agreement between two universities was made with the NURI
supervisors and HU members of the committee for international exchanges.
119
Cooperative Exchange with College of Ocean Science, Cheju National University (CNU),
Cheju, Korea
Objective of this cooperation is to establish research and educational collaboration between
CNU and Faculty of Fisheries Science, Hokkaido University (HU) in research fields of
aquaculture development and aquatic toxicology. The cooperation activity includes
exchange of research information and samples, besides holding educational and scientific
seminars. Three meetings (Korea-Japan, Japan-Korea Joint Meeting on Reproductive
Biology of Aquatic Animals) have been held for the promotion of science in aforementioned
research fields, in which current and future studies were presented and discussed by
researchers and students from both universities. A part of outcomes from this cooperation
were additionally presented at three seminars (Japan-Korea Joint Seminar on Fisheries
Sciences).
Year
2004
2005
2006
Researchers
To Cheju
5
4
3
To Hakodate
4
3
4
Students
To Cheju
0
0
3
To Hakodate
1
1
0
2004 November
1st Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of
Aquatic Animals in Cheju, Korea.
2004 December
4th Japan-Korea Joint Seminar on Fisheries Sciences in Sapporo, Japan.
2005 November
2nd Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of
Aquatic Animals in Kumamoto, Japan.
2005 August
5th Japan-Korea Joint Seminar on Fisheries Sciences in Kunsan, Korea.
2006 November 3rd Korea-Japan, Japan-Korea Joint Meeting on Reproductive Biology of
Aquatic Animals in Cheju, Korea.
2006 August
6th Japan-Korea Joint Seminar on Fisheries Sciences in Hakodate, Japan.
120
Cooperative Exchange with College of Fisheries, Chonnam National University (CNU),
Yosu, Korea
Objective of the cooperation is to establish fruitful system of research and educational
collaboration for the area of safe and reliable marine food production in Korea and Japan.
Year
2004
2005
2006
Researchers
To CNU
6
5
1
To HU
9
5
0
Students
To CNU
0
0
0
To HU
0
0
0
2004 May
Japan-Korea Joint Seminar on fish diseases in Yosu, Korea
2004 November
Associate professor Dr. Nishizawa of HU, stayed two months at Yosu
National University (CNU at present) for collaborative research on
“pathogenesis of fish viral diseases”
2005 January
Associate professor Dr. Nishizawa of HU stayed two months at Yosu
National University (CNU at present) for collaborative research on
“pathogenesis of fish viral diseases”
2005 April
Kim wi-sik, who were from Yosu National University (CNU at present)
entered graduate school of fisheries sciences, Hokkaido University
2005 October
Professor Dr. Oh of Yosu National University (CNU at present) were
invited for 4th International COE Symposium “Marine Bio-Manipulation
Frontier for Food Production” at Hakodate, Japan.
2006 February
Associate professor Dr. Nishizawa of HU visited Yosu National University
(CNU at present) for collaborative research on “pathogenesis of fish viral
diseases”
2006 October
Professor Dr. Oh of CNU visited Hakodate, Japan for collaborative
research on “pathogenesis of fish viral diseases”
2007 June
Japan-Korea Joint Seminar on fish diseases in Yosu, Korea. Associate
professor Dr. Nishizawa of HU gave a special lecture as an invited lecturer
of KOSEF (Korean Science and Engineering Foundation)
121
Cooperative Exchange with Warmia and Mazury University (WMU), Olsztyn, Poland
Objective of the cooperation is to exchange academic idea on fish and shellfish genetics for
conservation of aquatic genetic resources. Prof. Alicja Boron was first invited to HU as the
JSPS (Japan Society for the Promotion of Science) short-term research fellow from June 1 to
July 30, 2005. In this term, she made two COE special seminars about “Present Status of
Inland Fisheries in Poland (June 23, 2005)” and “Taxonomy and Biology of Some Cobitis
Fish (Pisces:Cobitidae) (July 23, 2005)” and also conducted collaborative researches on fish
molecular cytogenetics and population genetics with the COE members, Prof. K. Arai and
Prof. S. Abe and the paper based on the co-operative research on loach genetics has already
accepted in the international journal (Genetica). In June 2006, Prof. S.Abe visited
Department of Zoology, WMU, and made a lecture. Collaborative research on atypical
reproduction in some fishes is now going.
Year
2004
2005
2006
To WMU
0
0
1
Researchers
To HU
0
1
0
To WMU
0
0
0
Students
To HU
0
0
0
2002 September Prof. K. Arai of HU visited WMU for the 2nd International Conference on
Loaches of the genus Cobitis and related genera.
2005 June
Prof. A. Boron of WMU visited HU as a JSPS short-term research fellow
and stayed until the end of July for collaborative research with K.Arai and
S.Abe.
2006 June
Prof. S.Abe of HU visited WMU and made a lecture.
2007 May
Paper based on collaborative research was accepted to the International
Journal, Genetica.
122
Cooperative Exchange with Department of Zoology, North Carolina State University
(NCSU), Raleigh, NC, USA
Objective of this cooperation is to establish research and educational collaboration between
North Carolina State University (NCSU), Department of Zoology and Hokkaido University,
Faculty of Fisheries (HU) in research fields of fish reproductive physiology and aquatic
toxicology. The cooperation activity includes exchange of research information and samples,
as well as exchanging students for a short term in order to learn research skills. A part of
outcomes from this cooperation were presented at COE seminars.
Year
2004
2005
2006
Researchers
To North Carolina
0
0
1
To Hakodate
0
4
0
Students
To North Carolina
1
0
0
To Hakodate
0
0
1
2004 March
Research meeting with Dr. Craig V. Sullivan (William Neal Reynolds
Distinguished Professor at NCSU, Dept. Zoology), at Hawaii, USA.
Subjects: “Collaboration in research on fish reproductive physiology and
aquatic toxicology” and “Exchange of students between NCSU and HU”.
2004 April
Exchange a HU graduate student (M.sc.) for ~2 weeks. Subjects:
“Spawning and culturing striped bass” and “Sampling blood samples from
grey mullet”, at Pamlico Aquaculture Field Laboratory, NCSU, NC, USA.
2005 February
Research meeting with Professor Sullivan in Hakodate, Japan. Two
special lectures by Professor Sullivan at COE special seminar, Hakodate,
Japan.
Attendance of Professor Sullivan (special guest) to 1st International COE
Symposium, Sapporo, Japan.
2005 October
Research meeting with Professor Sullivan in Hakodate, Japan.
Attendance of Professor Sullivan (special guest) to 4th International COE
Symposium, Hakodate, Japan.
2006 March
Research meeting with Professor Sullivan and faculty members at Dept.
Zool., NCSU, NC, USA.
2006 August
Exchange a NCSU undergraduate student (senior) for ~6 weeks.
Subjects: “Immunological and biochemical analyses on fish serum and egg
yolk proteins”, at HU, Hakodate, Japan.
123
Appendix 7
Educational Achievements
Graduate School Curriculum
page 125
see page 94 Appendix 5
COE Special Lectures
page 127
Academic Exchange Agreements
Academia-Industrial Joint Activities
page 130
Number of Degree Recipients and Place of Employment
page 132
124
Graduate School Curriculum
Graduate School is consisted of two divisions, Marine Bioresource and Marine Life Science.
Students can select two courses, Advanced Course and Interfield Course. The Advanced
Course is aimed to foster researchers in the specific field, whereas the Interfield Course is
aimed to foster specialists who have an international and broad outlook in the field of
fisheries sciences. Each Division consists of 6 Chairs that contains 2-3 specific research
fields as follows.
Division
Chairs
Chair of Marine Biology and
Biodiversity
Chair of Marine Bioresource
Science
Division of
Marine
Bioresource and
Environmental
Science
Chair of Marine Environmental
Science
Chair of Marine Bioresource and
Environment Sensing
Chair of Marine Industrial Science
and Technology
Chair of Strategic Studies on Marine
Bioresource Conservation and
Management (Fixed term contract)
Chair of Aquaculture Biology
Chair of Aquaculture Genetics and
Genomics
Division of
Marine Life
Science
Chair of Marine Biotechnology and
Microbiology
Chair of Marine Bioresources
Chemistry
Chair of Marine Products and Food
Science
Chair of Marine Biosafety Science
and Technology
(Fixed term contract)
Fields
Field of Planktology
Field of Benthology
Field of Systematic Ichthyology
Field of Marine Bioresource Ecology
Field of Marine Bioresource Production
Field of Theoretical Ecology
Field of Physical Environment Science
Field of Chemical Environment Science
Field of Satellite Fisheries Oceanography
Field of Acoustical Fisheries Oceanography
Field of Fisheries Resource Management
Field of Fisheries Behavioral Research
Field of Fisheries and Marine Technology
Field of Fisheries Economics and Marine Business
Field of Fisheries informatics
Field of Fisheries System and Life Sciences of Regional
Resources
Field of Marine Bioresource Management Strategy
Field of Hydrosphere Conservation Ecology
Field of Comparative Physiology
Field of Reproductive Physiology
Field of Functional Biochemistry
Field of Plant Aquaculture Genetics
Field of Animal Aquaculture Genetics
Field of Aquatic Bioresource Genetics
Field of Marine Microbiology
Field of Marine molecular Biology
Field of Aquatic Epizootiology
Field of Bio-functional Chemistry
Field of Bio-analytical Chemistry
Field of Bio-molecular Chemistry
Field of Marine Bioresources Utilization
Field of Food Biochemistry
Field of Food Functional Chemistry
Field of Food Safety
Field of Marine Environmental Biosafety Management
Field of Marine Life Safety and Quality Management of
Food
125
Lectures are consisted of 13 categories as follows. The characteristic features of the
curriculum are inter faculty, interdivision, interchair and interfield lectures, which support
fostering specialists who have an international and broad outlook in the field of fisheries
sciences. For example, COE seminars held once in a month are credited as the
“Interdivision Lecture.” In the COE seminar, the cutting-edge scientists invited from the
world and researchers from the private companies have given lectures. Integration of the
COE seminar into the curriculum makes the lectures up to date, and the curriculum becomes
much flexible.
Lecture Category
Interfaculty
Lecture
Interdivision
Lecture#
Interchair Lectures
Interfield Lectures
Advanced Lectures
Division of Marine Bioresource and
Marine Life Science
Lecture name (Credits)
Introduction of Fisheries Science in Japan (2)
Fisheries Science Update in Japan (2)
Marine Biology and Biodiversity (2)
Marine Bioresource Science (2), etc.
Planktology (2), Benthology (2)
Satellite Fisheries Oceanography (2)
Marine Bioresource Management
Strategy (2), etc.
Marine Biology and Biodiversity I (4)
Marine Bioresource Science I (4), etc.
Lob. Works
Marine Biology and Biodiversity II (6)
Marine Bioresource Science I (6)
Marine Bioresource Science II (6), etc.
Literature Reading
Special
Lectures#
Advanced
Seminars*
Research Work*
Lecture name (Credits)
Marine Environmental Science (2)
Seminars
Training Courses
Division of Marine Life Science
Marine Bioresource Science I (4)
Marine Bioresource Science II (4), etc.
Marine Bioresource and Marine Life
Science (4)
Marine Bioresource and Marine Life
Science (2)
Marine Biology and Biodiversity III (2)
Marine Bioresource Science II (2)
Marine Bioresource Science III (2), etc.
Marine Biology and Biodiversity (10)
Marine Bioresource Science (10), etc.
Fisheries Science Update in Japan (2)
Aquaculture Biology (2), Aquaculture
Genetics and Genomics (2), etc.
Comparative Physiology (2)
Reproductive Physiology(2)
Animal Aquaculture Genetics (2)
Aquatic Epizootiology (2), etc.
Aquaculture Biology I (4)
Aquaculture Genetics and Genomics I
(4), etc.
Aquaculture Biology I (6)
Aquaculture Biology II (6)
Aquaculture Genetics and Genomics I
(6)
Aquaculture Genetics and Genomics II
(6), etc.
Aquaculture Biology (2)
Aquaculture Genetics and Genomics (2)
Marine Life Science (4)
Marine Life Science (2)
Aquaculture Biology II (2)
Aquaculture Biology III (2)
Aquaculture Genetics and Genomics II
(2)
Aquaculture Genetics and Genomics III
(2), etc.
Aquaculture Biology (10)
Aquaculture Genetics and Genomics
(10), etc.
#Lectures that the COE program actively contributes
* For Doctoral Course students
126
Please refer Appendix 5 for programs of the symposia.
COE Special Lectures
Total 33 special lectures have conducted in FY 2004-2006. Seven of them were conducted by
foreign invited researchers, as listed below.
February 23, 24, 2005
Craig V. SULLIVAN (North Carolina State University, USA, Professor)
“Aquaculture of Hybrid Striped Bass”
“Oogenesis in Teleost Fishes”
March 1, 2005
Gary H. THORGAARD (Washington State University, USA, Professor)
“Salmon Conservation in the Pacific Northwest USA”
John A. H. BENZIE (University of New South Wales, Australia, Visiting Professor)
“Coral Reef Biology and Biotechnology in Australia”
Zhiyuan GONG (National University of Singapore, Singapore, Associate Professor)
“The Current Situation in Commercialization of Transgenic Fish and Our Experiments on
Reproductive Disadvantages of the Fluorescent Transgenic Fish”
“Road Show about National University of Singapore”
Joseph G.. CLOUD (University of Idaho, USA, Professor)
“Salmonid Reproductive Biology: Supporting Idaho Aquaculture”
May 24, 2005
Zigang DONG (Minnesota University, USA, Professor)
“Cell Molecular Biology on Antitumor Substances in Natural Food Products”
Kenji SATO (Kyoto Prefectural University, Japan, Professor)
“Development of Peptides Purification Methods from Marine Organisms and Effects of Such
Peptides on Tumor Cell Proliferation and Scar Regeneration”
Michael T. MORRISSEY (Oregon State University, USA, Professor)
“Sea Food Laboratory in the Oregon State University”
127
May 25, 2005
Shukun Yu (Senior Scientist, PhD, Danisco A/S, Copenhagen K, Denmark)
“The Discovery of New enzymes, New products and a New Pathway from Marine Red
Algae”
June 23, 2005
Alicja BORON (University of Warmina and Mazury, Poland, Professor)
“Present Status of Inland Fisheries in Poland”
July 22, 2005
Alicja BORON (University of Warmina and Mazury, Poland, Professor)
“Taxonomy and Biology of some Cobitis fish (Pisces, Cobitidae) ”
Björn Th. BJÖRNSSON (Göteborg University, Sweden, Professor)
“Growth hormone endocrinology of salmonids ”
Kristina SUNDELL (Göteborg University, Sweden, Professor)
“Intestinal physiology and barrier function”
September 14, 2005
Yoon Kwon NAM (Pukyong National University, Professor)
“Recent Activities on Marine and Aquatic Biotechnology in Korea”
October 12, 2005
Ruth B. PHILLIPS (Washington State University, USA, Professor)
“Evolution of Salmonid Sex chromosomes”
Anthony G. GHARRETT (University of Alaska Fairbanks, USA, Professor)
“Effective Population Soze, the Genetic Relaity of Enumerating Fish”
James E. SEEB (Alaska Department of Fish and Games, USA, Senior Scientist)
“Alaskan Fisheries and Genetics”
128
Academic Exchange Agreements
Please refer Appendix 6.
129
Academia-Industrial Joint Activities
Academia-Industrial Seminars
Through the Academia-Industrial Seminars, the COE program is supporting exchanges among
graduate students and researchers in the private companies to foster mutual understandings.
The aim of the seminars is to show various carrier path models to the students to help them
establishing their future planning.
Three seminars were held in 2005-2006.
2005 July
Ken IGARASHI (Leica Microsystems): High resolution analysis of
ultrastructure and material transport in the cell using filter-less Confocal
laser scanning microscopy.
2006 February
Shogo MORIYA (Nisshinbo Research and Development Center):
Development of the eye-observable microarray system.
2006 December
Osamu NAKAYAMA (Applied Biosystems Japan): Real-time quantitive
PCR: its mechanism of quantification and application possibility.
Open lectures in Hakodate and other outreach efforts
Faculty of Fisheries Sciences, Hokkaido University, has held Open Lectures in Hakodate.
The members of the COE program have actively supported the lecture as lecturers.
2004 “Development of New Fisheries Science”
Shinji ADACHI: Cutting edge of fisheries science and its contribution to regional
economy
Mamoru YOSHIMIZU
Cutting edge of epizootiology
2005 “Research Goal of Fisheries Science in the Faculty”
Kohei YAMAUCHI Research in the field of Fisheries Sciences and contribution to
society
Katustoshi ARAI
Objectives of the 21st COE program in the Faculty of Fisheries
Mamory YOSHIMIZU
How do we secure safety of seafood
Etsuro YAMAHA Biotechnology in Fisheries Science
2006 “Marine Environment and Bioresources: what is going in the sea?”
HIroyuki MIZUTA Growth and reproduction of kelp and marine environment
130
2007 “Cutting Edge of Fisheries Science that Brings Safety and Reliability in Seafood
Industry”
Mamoru YOSHIMIZU
Quality control in seafood ~ Salmon and oyster as
examples ~
Takao OJIMA Production of useful enzymes from wastes of seafood industry
~ Extraction of cellulase from digestive caecum of scallops ~
The COE program has held some outreach programs
2005 March Hokkaido University Open Forum in Hakodate ~ From Ocean, Hakodate
and Hokkaido, Our Health and Safety and Reliability of Seafood ~
2006 March Cutting Edge of Life Sciences in Hakodate Area (a meeting for announcing the
results of the COE program and Cooperation of Innovative Technology and
Advanced Research in Evolutional Area (CITY AREA))
131
Number of degree recipients and Place of Employment
(Graduate School of Fisheries Sciences, Division of Marine Life Science)
FY 2004
M2
D3
National & public institute
0 (0)
0 (0)
University
1 (1)
4 (3)
Public office
1 (1)
0 (0)
Private company
31 (14)
0 (0)
Higher education
(Graduate School of
Fisheries Sciences)
7 (6)
0 (0)
Higher education (Others)
3 (0)
0 (0)
Others
4 (4)
8 (6)
Total number of
degree recipients
47 (26)
12 (9)
Numbers in parentheses indicate place of employment of graduates who were supervised by
COE members.
132
FY 2005
M2
D3
1 (1)
0 (0)
University
0 (0)
6 (5)
Public office
3 (2)
0 (0)
Private company
31 (14)
3 (2)
Higher education
(Graduate School of
Fisheries Sciences)
7 (5)
0 (0)
2 (2)
0 (0)
Others
6 (5)
3 (1)
Total number of
degree recipients
50 (29)
12 (8)
COE members.
133
FY 2006
M2
D3
0 (0)
0 (0)
University
0 (0)
3 (3)
Public office
0 (0)
0 (0)
Private company
45 (21)
0 (0)
Higher education
(Graduate School of
Fisheries Sciences)
6 (5)
0 (0)
1 (1)
0 (0)
Others
2 (2)
7 (6)
Total number of
degree recipients
54 (29)
10 (9)
COE members.
134
Appendix 8
Achievements in Young Scientist Fostering
PD Fellowship
RAship
Onuma Workshop for Young Scientists
page 137
COE Poster Presentation Awards in International Symposia
page 141
Traveling Awards for International Conferences
page 143
135
Post-doctoral Fellowship
Please refer Appendix 1 for members of post-doctoral fellows.
Research Asssitantship
Please refer Appendix 1 for members of research assistants (doctor course students).
136
Onuma Workshop for Young Scientists
The COE program has held 3 workshops for young scientists in FY 2005-2007. The
workshop is aimed to foster young scientists in various points of views. In the workshop,
special lectures and trainings were given. Programs are listed below.
The 1st Onuma Workshop for Young Scientists
“Visions of young and female scientist lead to frontier of fisheries sciences”
Date: September 6-8, 2005
Venue: Greenpia Onuma
229, Akaigawa, Mori, Minamikayabe, Hokkaido
Schedule & Program
September 6, Tuesday (Day 1)
09:00Departure from the Sapporo Campus, Hokkaido University (participants from Sapporo)
11:30-13:00
Visit to the Muroran Marine Station, Field Science Center for Northern Biosphere,
Hokkaido University
Lunch
16:00-17:00
Arrival at the hotel, Greenpia Onuma (all participants)
Registration
17:00Workshop Opening
Opening Remarks:
Gender equality in science and technology field.
Noriko Shiomitsu (Chief of Research Section, Gender Equality Bureau, Cabinet
Office)
Keynote Speeches:
Professional with flexibility: to become a female scientist.
Akiko Tsugawa (Professor, Tokyo Medical and Dental University)
Physiological study of prawns and its application for aquaculture.
Marcy Wilder (Senior Research Scientist, Japan International Research Center for
Agricultural Sciences)
It needs “physical strength” to make career as a researcher while raising kids
Rie Goto (Research Fellow, 21st Century COE Program “Marine Bio-Manipulation
Frontier for Food Production”)
Welcome Cocktail
September 7, Wednesday (Day2)
09:30-14:00
Training Course of Fish Biology at the Nanae Fresh-Water Laboratory, Field Science
Center for
Northern Biosphere, Hokkaido University
137
1. Artificial fertilization of masu salmon
2. Dissection of fish and squid
3. Goldfish and trout fishing
Lunch
16:00Evening Session
Friendship Mixer
September 8, Thursday (Day3)
09:00Concluding Remarks
10:00Departure from the hotel
10:45Visit to the Hakodate Campus (Faculty of Fisheries Sciences), Hokkaido University
Lunch
13:00Departure from Hakodate (participants from Sapporo)
18:00Arrival at the Sapporo Campus
The 2nd Onuma Workshop for Young Scientists
“How to make your work career after Ph.D. course”
Schedule & Program
September 5, Tuesday (Day 1)
09:00Departure from the Sapporo Campus, Hokkaido University (participants from Sapporo)
11:30-13:00
Visit to the Muroran Marine Station, Field Science Center for Northern Biosphere,
Hokkaido University
Lunch
16:00-17:00
Arrival at the hotel, Greenpia Onuma (all participants)
Registration
17:00Workshop Opening
Special Lectures; “My work career after getting Ph.D. degree”:
1. Ivo Galli (European Patent Office; Principal Examiner)
138
2. Mari Jibu (National Institute of Science and Technology Policy; Senior Researcher)
3. Yasushi Hirayama (Nichirei Co., Ltd.; Manager)
4. Hiromi Yokoyama (Science Writer)
5. Testuo Tsuda (Mitsubishi UFJ Securities Co., Ltd.)
6. Taiki Susa (Management Center of Intellectual Property, Hokkaido University;
Manager)
7. Katsumi Doi (Japan Science and Technology Agency; Fellow)
19:00Welcome Cocktail
September 6, Wednesday (Day2)
Skill Up Lectures by Communicators in Science and Technology Education Program
(CoSTEP), Hokkaido University
10:00Lecture 1: Communications. Miho Nanba (CoSTEP, Hokkaido University)
12:00Lunch
13:30Lecture 2: How to write a proposal of research grant. Jiro Sugiyama (CoSTEP, Hokkaido
University)
14:15Break
14:30Lecture 3: Broadcasting scientific information. Kunihiko Kumamoto (CoSTEP, Hokkaido
University)
15:30Break
16:00Work and Discussion by Groups: “Do not miss the turning point on your work career”
18:30Friendship Mixer
September 9, Thursday (Day3)
10:00Departure from the hotel
10:30Excursion to the Onuma Seminational Park and the Hakodate Campus (Faculty of Fisheries
Sciences), Hokkaido University
12:00Lunch
13:00Departure from Hakodate (participants from Sapporo)
18:00Arrival at the Sapporo Campus
139
The 3rd Onuma Workshop for Young Scientists
“Self-promotion to be a professional”
Schedule & Program
September 13, Thursday (Day 1)
13:00-14:00
Registration
14:00-15:00
Poster Session I
15:30Special Lectures:
8. Kazuhiro Ohsue (Asubio Pharma Co., Ltd.; Director)
9. Tishihiko Isimura (Shiseido Co., Ltd., Research and Development; Director of
Planning Department))
10. Hiromi Kawakami (Writer)
18:00Dinner
20:30-22:00
Evening Discussion
September 14, Friday (Day2)
Skill Up Lectures by Communicators in Science and Technology Education Program
(CoSTEP), Hokkaido University
09:00Lecture 1: How to make a good poster
Kunihiko Kumamoto (Professor, CoSTEP)
Gensei Ishimura (Associate Professor, CoSTEP)
Schuko Ohtsu (Research Assistant, CoSTEP)
11:00Group Works: Physical and Mental Training
12:30Lunch
14:00Lecture 2: Practical training of poster presentation for self-promotion
16:00-17:30Poster Session II
18:00Dinner
20:30-22:00
Evening Discussion
September 15, Saturday (Day3)
140
COE Poster Presentation Awards
In order to train young scientists, poster sessions for graduate students and post docs have
been held in the COE International symposia. In the sessions, established researchers
attended in the symposia are requested by the organizers to actively discuss with students and
post docs. After the session, poster presentation awards are given to encourage young
scientists.
2005 February
1st International COE Symposium “Potential and Perspective of Marine Bio-Manipulation”
Best Poster Award
SAKAO S Characteristics of primordial germ cells and the cause of larval mortality in
tetraploid masu salmon induced by the first cleavage inhibition.
Good Poster Awards
SENAHA A Effect of androgen on the expression of lipidation related genes in female
Japanese eel (Anguilla japonica).
SAITO T Visualization of primordial germ cells in various bony fish embryos.
SUZUKI K
Biochemical properties of abalone cellulases.
OTSUKA S Isolation and cDNA cloning of exo-type mannuronate lyases from the
pacific abalone Haliotis discus hannai
2005 May
2nd International COE Symposium “Marine resources as Functional and Therapeutic Agents”
Best Poster Award
KONISHI I Apotosis induction by carotenoids isolated from Halocynthia roretzi on
human cancer cells.
Good Poster Awards
MAEDA H Anti-obesity effects of Undaria pinnatifida (Wakame) lipid.
SHIRAHATA K
cells.
The effect of polyunsaturated fatty acids on differentiation of 3T3-L1
2005 October
4th International COE Symposium “Reproductive, Genetic and Disease Management in
Aquaculture and Ocean Ranching”
Best Poster Award
SAWAGUCHI S Molecular characterization of three forms of vitellogenin and their
141
yolk protein products during oocyte growth and maturation in red seabream, Pagrus
major.
Good Poster Awards
KUBOTA Y Sea urchin vasa-related genes: specific markers of the germline in
Strongylocentroutus intermedius.
YAMAGUCHI N In vitro induction of gonadal sex change in protogynous fish.
TOSAKA R Localization of androgen receptor mRNA in gonads of Japanese eel
(Anguilla japonica).
AMANO H Multiple lipovitellins in grey mullet: N-terminal amino acid sequencing
and mapping of their constituent polypeptides.
TOHSE H Identification of starmaker-like protein gene and its possible function on
inner ear development and otolith biomineralization.
ITO D Chromosome abnormalities of inviable hybrids between chum salmon and
Japanese huchen.
SHIMIZU T Biological control of fish viral diseases with anti-viral substances
produced by bacteria.
2006 July
5th International COE Symposium “Industrial-Academia-Governmental Collaboration for the
Establishment of Marine food Production in China and Japan ”
Best Poster Awards
FUKUDA M Study on functional amino-acid residues of abalone alginate lyase by
site-directed mutagenesis.
HONG L Immunochemical detection of choriogenins (precursors to vitelline envelope)
in grey mullet (Mugil cephalus).
Good Poster Awards
NAKAMURA A Extraction of tropomyosin from dried scallop by boiling and its
allergenicity.
HATA M
Comparative study on alginate lyases from marine mollusks.
SHIMIZU T Manipulating diets with anti-viral substance-producing bacteria for seed
production of marine fish.
Special Awards
GAO J Studies on relation of supply-demand of farming fishery products in China.
ZHAO J
Phylogeny of the Sinipercine fishes endemic in East Asia.
142
Traveling Awards for International Conferences
The COE program supports attendance of graduate students to international conferences and
short visit to foreign universities/public institutes. Traveling awards have been awarded to
the following students and post-docs.
Year
Name
SUZUKI K
2004
SHIN D
Purpose etc
The 6th Asia-Pacific Marine Biothechnology Conference, Zhoushan,
China. Poster presentation entitled “Production of oligoalginates by
the digestion of Laminaria with abalone alginate lyase”
7th Asian Fisheries Forum, Penang, Malaysia. Poster presentation
entitled “Induction of early oocyte growth by the oral administration of
androgen in Japanese eel, Anguilla japonica”
YUAN C
Visiting Shanghai Fisheries University
SHIN D
Visiting aquaculture seed production facilitiesin Korea
NAKAI H
SABURI W
YUAN C
SHEN X
2005
TANG Y
OTSUKA S
PARK E
KASAI H
6th Carbohydrate Bioengineering Meeting, Barcelona, Spain. Poster
presentation entitled “Functional analysis in glycoside hydrolase
family 31 enzymes from rice seeds (Oryza sativa L. var Nipponbare)”
6th Carbohydrate Bioengineering Meeting, Barcelona, Spain. Poster
presentation entitled “Reaction of α-glucosidase on
1,5-anhydrofructose”
3rd COE International Symposium
“Industrial-Academia-Governmental Collaboration for the
Establishment of Marine food Production in China and Japan ”,
Shanghai, China
Shanghai, China
Shanghai, China. Poster presentation entitled “Measuring abundance
of pelagic fish school using sonar”
Marine Biotechnology Conference 2005, Newfoundland, Canada.
Poster presentation entitled “Isolation and Characterization of a Novel
endo-β-1、4-Mannnanase from the Pacific Abalone Haliotis discus
hannai”
Marine Biotechnology Conference 2005, Newfoundland, Canada.
Poster presentation entitled “CAPS analysis of genetic diversity in
Porphyra yezoensis-related species (Bangiales、Rhodophyta) from
Japan and Korea”
12th Internatinal Conference of the Europian Association of Fish
Pathologists, Copenhagen, Denmark. Poster presentation entitled
“Survivability of koi herpesvirus (KHV) in environmental water as
well as physical and chemical inactivation” Poster Presentation
Award
143
SAITO T
GOTO-KAZETO
R
MORISHIMA K
NAKAI H
2006
HONG L
SHIN D
IIMUA K
CHOI S
The 7th Internatinal Conference of “Zebrafish Development &
Genetics”, Madison, USA. Poster presentation entitled “Germ-line
replacement between different species by transplantation of a single
primordial germ cell”
The 7th Internatinal Conference of “Zebrafish Development &
Genetics”, Madison, USA. Poster presentation entitled “Efficient
Isolation of Primordial Germ Cells in Zebrafish by Cell Sorting”
The 9th Genetics in Aquaculture Symposium, Montpellier, French.
Poster presentation entitled “HYBRIODGENESIS-LIKE
OOGENESIS IN TRIPLOID FEMALES DERIVED FROM THE
CLONE LINEAGE OF MISGURNUS LOACH”
7th Carbohydrate Bioengineering Meeting, Branschweig, Germany.
Poster presentation entitled “Ion-dependent α-Glucosidase from
Ligament and Digestive Caecum of Scallop”
Sampling of mullet in China
2006 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture,
Busan, Korea. Poster presentation entitled “Artificial control of
reproduction for aquaculture and conservation in sturgeon”
2006 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture,
Busan, Korea. Poster presentation entitled “Molecular tools to study
scale forming cell differentiation -cDNA cloning and
expressionvpatterns of BMP2, Runx2 and SPARC-“
International Symposium of Korean Society Phycology in Honor of
the 20th Anniversary, Seoul, KOrea. Poster presentation entitled
“PORPHYRA YEZOENSIS AS A MODEL PLANT IN MARINE
BIOSCIENCES -THE STATE AND PROSPECT”
144

Appendices

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