Unravelling the cause of Black Pod Syndrome of narrow
Transcription
Unravelling the cause of Black Pod Syndrome of narrow
Monica Kehoe School of Plant Biology and The UWA Institute of Agriculture, Faculty of Science Monica completed a Bachelor of Science with a major in Microbiology at The University of Melbourne, and moved to Western Australia in 2005 to work in Plant Virology at the Department of Agriculture and Food WA (DAFWA). Her fascination with (plant) viruses and her interest in research led to an honours degree at Murdoch university, where she worked on resistance phenotypes to Turnip mosaic virus in mustard species. She commenced her PhD at UWA in 2011, working on Black Pod Syndrome in lupins. She continues to be based at DAFWA where she has participated in a wide range of research projects, including viruses in broadacre and agricultural crops. The University of Western Australia THE UWA INSTITUTE OF AGRICULTURE Postgraduate Showcase 2013: Frontiers in Agriculture Unravelling the cause of Black Pod Syndrome of narrow-leafed lupins Monica Kehoe The University of Western Australia Acknowledgements Supervisors: Roger Jones and Bevan Buirchell DAFWA: Brenda Coutts, Stuart Vincent and Eva Gajda GRDC for funding (GRDC supported PhD Project GRS10039) The University of Western Australia Australia grows 85% of global crop, WA 80% of national crop 2005-2010 average area was 540,000 ha, av. yield 1.2 t/ha Based on soils and climate, 1.6 million ha possible Black Pod Syndrome major reason yields below potential, especially in south coast zone Lupin production zones The University of Western Australia Pods, especially primaries, turn black or dark brown producing few or no seeds Causes other than virus now ruled out Black Pod Syndrome (BPS) BPS and late Bean yellow mosaic virus (BYMV) infection coincide Differences between cultivar plots The University of Western Australia BYMV in narrow-leafed lupins Early infection Spread by aphids Nearby pasture Necrosis, shepherds crook, death Nbm-1 gene Typical early season BYMV symptoms The University of Western Australia Serological (ELISA) Molecular (PCR) Green leaf Necrotic stem The University of Western Australia Initial glasshouse studies reproduced BPS in plants inoculated with BYMV The University of Western Australia 2011 survey 5 locations Testing by ELISA and PCR 133 plants – 72 symptomatic Typical BPS in the field Multiple samples per plant - 314 Best spot to test = stem, below black pods BPS symptomatic samples The University of Western Australia Number ELISA PCR Section of plant of samples POTY BYMV POTY BYMV Stem (near black pods) 72 75% 83% 99% 82% Stem (mid) 72 33% 61% 89% 58% Leaf 48 2% 19% 96% 48% The University of Western Australia Reproducing BPS in the glasshouse Mandelup vs Jenabillup 8 plants per cultivar inoculated every two weeks Infection before flowering = typical BYMV symptoms: Test Test vs Control The University of Western Australia Infection at primary pod stage – black pod and plant death The University of Western Australia Infection after 1° pods fully developed and 2° and/or 3° pods formed = typical BPS symptoms The University of Western Australia Drawback: it takes 6 months (and a whole glasshouse) to get here! The University of Western Australia Koch’s Postulates Lupin BPS samples positive for BYMV BPS symptoms occur BYMV inoculate to other host BYMV used to infect healthy lupin BYMV detected in formerly healthy lupin The University of Western Australia THE UWA INSTITUTE OF AGRICULTURE Postgraduate Showcase 2013: Frontiers in Agriculture Summary BYMV causes BPS Time of infection critical for BPS symptoms Is the Nbm-1 gene responsible? Can closely related viruses cause BPS? Solution? The University of Western Australia