Upstream Processes

Transcription

Upstream Processes

American Chemical Society
Division of Biochemical Technology
st
251 ACS National Meeting
San Diego, California March 13 -17, 2016
2016 Program Grid
8:30-11:30 am A
B
C
D
13-Mar
Sunday
14-Mar
Monday
15-Mar
Tuesday
16-Mar
Wednesday
17-Mar
Thursday
BIOT Symposia
Disruptive Bioprocessing:
Upstream Processing
(Crystal I)
Mammalian Cell Culture
Process Development II
(Crystal II)
Engineering Non-Model Hosts
for Biological Production
(Diamond II)
Advances in Systems Biology
(Crystal II)
Engineering Natural Products
Biosynthesis
(Crystal II)
Upstream
Downstream Processing for
Vaccines, Gene Therapy
Vectors and Non-Protein
Biological Products
(Emerald Ballroom)
Advances in Chromatographic
Separations I
(Emerald Ballroom)
Antibodies, Drug Conjugates
and Related Molecules I
(Emerald Ballroom)
Leveraging Fundamentals for
Accelerated Downstream
Process Development
(Emerald Ballroom)
Non-Chromatographic
Separations and Process
Integration I
(Emerald Ballroom)
Downstream
Imaging, Sensing, and
Bioactuation
(Diamond I)
Protein Engineering and
Design I
(Diamond I & II)
Protein Therapeutics:
Discovery and Production
(Diamond I)
Protein Conjugates and
Materials I
(Diamond I)
General Topics in Biomolecular
and Biophysical Processes
(Diamond I)
Biophysical
Biomolecular
New Tools and Approaches I
(Opal)
New Tools and Approaches II
(Opal)
Disease and Biomedical
Applications I
(Opal)
Emerging
Technologies
Bio-Design and Systems
Analyses at the Cellular Scale
(Crystal I)
Development and
Manufacturing Considerations
for Biosimilars
(Crystal I)
QbD Case Studies: Process
Characterization
(Crystal I)
Lifecycle Management
(Crystal I)
Biosimilars
Biocatalysis and
Biotransformations
(Pearl)
Metabolic Engineering and
Synthetic Biology:
Pathways/Products
(Crystal II)
Protein Structure and Function
(Diamond II)
Control of Protein Quality
Attributes
(Diamond II)
Biofuels
David Perlman Memorial
Lectureship & Van Lanen
Award
(Emerald Ballroom)
Marvin J. Johnson Award in
Microbial & Biochemical
Technology
(Emerald Ballroom)
BIOT Young Investigator Award
& Peterson Award
(Emerald Ballroom)
GE Healthcare Seminar
(Topaz)
Bio-Rad Seminar
(Topaz)
Future Programming Meeting
(Crystal I)
Mammalian Cell Culture
Process Development I
(Crystal II)
Microbial Process Development
(Crystal II)
Disruptive BioProcessing:
Downstream Processing
(Emerald Ballroom)
Advances in Chromatographic
Separations II
(Emerald Ballroom)
Controlling the Interface of
Proteins, Cells and Materials
(Diamond I)
Protein Engineering and Design
II
(Diamond I & II)
Synthetic Biology Approaches
Engineering Microbes to Utilize
to Engineer Production of Fuels
Next Generation Feedstocks
and Energy Molecules
(Opal)
(Opal)
E
F
11:30 am-12:30 pm
12:30-2:00 pm
2:00 - 5:00pm A
B
C
Synthetic Biology and -OMICS
Approaches to Engineer
Microbial Communities
(Opal)
D
E
5:00 - 6:00pm
Biomolecular Design and
Biophysics
(Crystal I)
Disruptive BioProcessing:
Panel Discussion
Evening Events
Program Chair Lunch Meeting
(Pearl)
Computational
Biotech
QbD
Advances in Biocatalysis
(Crystal II)
General Topics in Upstream
Processes
(Crystal II)
Technology Transfer, Scale-up
and Scale-Down
(Emerald Ballroom)
Non-Chromatographic
Separations and Process
Integration II
(Emerald Ballroom)
Protein Therapeutics:
Formulation and Delivery
(Diamond I)
Protein Conjugates and
Materials II
(Diamond I)
Prediction and Characterization
of Biophysical Properties
(Diamond I)
Biomass Pretreatment and
Hydrolysis
(Opal)
Stem Cells and Regenerative
Medicine
(Opal)
Protein and Molecular
Engineering
(Opal)
Disease and Biomedical
Applications II
(Opal)
Process Modeling in
Biotechnology
(Crystal I)
Challenges in Demonstrating
Analytical Biosimilarity
(Crystal I)
QbD Case Studies: Process
Validation
(Crystal I)
Kavli lecture
Industrial Biotechnology Award
(Emerald Ballroom)
Biotechnology &
Bioengineering Daniel I. C.
Wang Award Lecture
(Emerald Ballroom)
SciMIx Poster Session
Convention Center
(Hall D/E)
8:00 - 10:00 pm
Poster session
Convention Center
(Hall E)
6:00 - 9:00 pm
Networking session
BIOT Student Chapter
Meeting
(Topaz)
6:00 - 10:00 pm
EX-COMM Meeting
(7:00 - 10:00 PM)
(Topaz)
Awards Dinner (7 pm)
(Emerald Ballroom)
BIOT Reception,
6:30-9:00 pm
BIOT Members Only
Metabolic Engineering and
Synthetic Biology: Tools
Development
(Crystal II)
Antibodies, Drug Conjugates
and Related Molecules II
(Emerald Ballroom)
Biotechnology &
Bioengineering Awards
Presentation and Gaden Award
Lecture
(Emerald Ballroom)
Table of Contents
Company Sponsors...........................................................pg. 6
Awards Presentations & Non-Technical BIOT Meetings...pg. 7
Westin San Diego Hotel Maps...................................................pg. 9
Sunday Reception...............................................................................pg. 11
Keynote Speakers..........................................................................................pg. 13
BIOT Division Award Winners.........................................................................pg. 16
Company Seminars..............................................................................pg. 20
Special Programming on Computational Biotechnology.........pg. 22
The Best of BIOT Awards...................................................pg. 23
Sessions
Sunday Morning............................................................pg. 24
Sunday Afternoon.........................................................pg. 27
Monday Morning.............................................................pg. 31
Monday Afternoon..............................................................pg. 35
Tuesday Morning.......................................................................pg. 39
Tuesday Afternoon.............................................................................pg. 43
Tuesday Evening...........................................................................................pg. 47
Wednesday Morning......................................................................................pg. 57
Wednesday Afternoon.......................................................................pg. 61
Thursday Morning.....................................................................pg. 65
Thursday Afternoon.............................................................pg. 69
Sponsored Company Ads...............................................pg. 73
Author Index....................................................................pg. 83
Convention Center Map.................................................pg. 95
ACS Shuttle Maps.............................................................pg. 98
Acknowledgment from the Division Chair
Dear Colleagues,
On behalf of the BIOT Executive Committee, I would like to welcome you to another
outstanding BIOT program at the 2016 ACS National Meeting. Program Chairs
Peter Tessier from Rensselaer Polytechnic Institute and Scott Tobler from Merck,
along with area coordinators, session chairs, our long-term program coordinator
Claire Komives, and many other volunteers have done an outstanding job of
creating this year’s program with nine excellent keynote lectures, seven award
lectures, and over 500 oral and poster presentations. This year’s meeting includes
53 technical sessions, as well as several networking opportunities for BIOT
members and colleagues, including the traditional BIOT reception on Sunday, an
evening reception after the poster session on Tuesday, and an evening networking
event on Wednesday.
This year Pete and Scott have organized programming in several BIOT Areas: Upstream Processes,
Downstream Processing, Biomolecular and Biophysical Processes, Biofuels, Emerging Technologies, QbD
and Biosimilars. In addition, special sessions focusing on Computational Biotechnology have also been
planned. The program chairs and area coordinators have worked behind the scenes for the last several months
to subscribe these programming areas with top-notch technical sessions full of high quality presentations.
The mission of this division, which is staffed entirely by volunteers, is to promote the exchange of
information among academic and industrial researchers regarding technology utilizing life-based systems
to produce useful products and services. To that end, BIOT and ACS offer many opportunities to serve the
biopharmaceutical and biotechnology community, including service on the Executive Committee and many
activities surrounding the technical programming for national meetings -- Program Chairs, Area Coordinators
and Session Chairs. I encourage you to get involved in division activities. If you are interested in contributing
to an exciting BIOT program at the next ACS annual meeting, please bring your ideas and suggestions to
the future programming/BIOT business meeting on Tuesday, March 15th, at 12:30. Please also feel free to
contact one of our Executive Committee members to learn how you can get involved in BIOT, and check our
website www.acsbiot.org for updates on our strategic planning discussions.
I would also like to acknowledge all of the BIOT Executive Committee members for their work to make BIOT
a valuable organization: Past-Chair Nitin Rathore, Chair-Elect David Roush, Secretary Anne Kantardjieff,
Treasurer Glen Bolton, Communications Coordinator Varnika Roy, Awards Chair Todd Przybycien,
Membership Chair Yonghyun (John) Kim, Web Programming Chair Nihal Tugcu, Councilors Arindam Bose,
Theresa Good, Sharon Shoemaker and Sadettin Ozturk, and Alternate Councilors Casim Sarkar, William
Kelly, Ranjan Srivastava, and Cynthia Collins.
I hope to see you during our time in San Diego. Please join me in thanking all the presenters and organizers
and our industrial sponsors for making this meeting another excellent opportunity for networking and exchange
of ideas. I hope you enjoy the meeting!
Acknowledgment from the Program Chairs
Dear BIOT members:
Welcome to the 251st ACS National Meeting in San Diego,
California!
This year’s technical program is one of the most extensive in
our meeting’s history, with eight topical areas encompassing
53 sessions of oral presentations, 9 keynote lectures, and a
poster session with over 150 posters. Building on the meeting’s
theme of “Computers in Chemistry”, there will be a special
symposium on “Computationally Enabled Biotechnology at the
Molecular, Cellular and Process Scales”. We will also recognize
the accomplishments of leaders in our field through seven award
lectures.
This year’s program also features numerous opportunities to reconnect with colleagues and make new
friends. Our Sunday evening reception for BIOT members at USS Midway in downtown San Diego will
provide appetizers and drinks in a unique venue. The Tuesday evening poster session will be accompanied
by a reception, and the Wednesday evening networking session will provide opportunities for younger BIOT
members to meet colleagues who are established in their fields.
In addition to the streamlined printed program, we are excited to again offer a free BIOT smartphone app.
Download the Guidebook app on the Apple App Store or Android Marketplace, and use redemption code
BIOT2016 to gain mobile access to the meeting schedule, complete abstracts and venue information, and
use the embedded feedback feature to provide comments on this year’s meeting. The technical program is
also available online through the ACS meeting website.
We are extremely grateful for the support and assistance of numerous individuals and sponsors. Program
planning would not have been possible without the dedicated efforts of a team of area coordinators, session
chairs, and ACS and BIOT support staff and executive committee members. Financial support from corporate
sponsors was also critical to our ability to host a first-rate meeting packed with keynote and award lectures,
invited talks, and networking and social events. These individuals and sponsors are highlighted in greater
detail in the acknowledgments pages that follow and throughout the program book.
The continued growth and vitality of the BIOT Division depends upon the enthusiasm of volunteer leaders.
If you are interested in contributing to future BIOT programs, please join us for the BIOT Business Meeting
and Future Programming Meeting at 12:30 PM on Tuesday in Crystal I. BIOT Division Chair Eric Boder and
Long-Range Program Coordinator Claire Komives are eager to discuss opportunities for you to become
more involved with the BIOT team. We trust you will enjoy all that this year’s program and the beautiful city of
San Diego have to offer, and we look forward to seeing you at the meeting.
Best Regards,
Eric T. Boder
2016 BIOT Division Chair
Pete Tessier, Rensselaer Polytechnic Institute
Scott Tobler, Merck
Acknowledgments
Company Sponsorship
We are extremely grateful to the many wonderful volunteers who donated their time and talent to
making this meeting a success. In particular we would like to acknowledge the efforts of a dedicated
team of area coordinators who worked with session chairs to plan the meeting.
ACS Synthetic Biology
Alexion Pharmaceuticals
Amgen
Avantor Performance Materials
Bio-Rad
Biogen
BioMarin Pharmaceutical Inc.
Biotechnology & Bioengineering
Boehringer Ingelheim
Bristol-Myers Squibb
GE Healthcare
Genentech
GlaxoSmithKline
Janssen Research & Development
KBI Biopharma
MedImmune
Merck and Co., Inc.
MilliporeSigma
Momenta Pharmaceuticals, Inc.
Northwestern Biotech MS Program
Novo Nordisk
Pall Life Sciences
Pfizer
Sanofi Genzyme
• Upstream Processes
Organized by Varnika Roy, Mark Blenner and Maciek Antoniewicz
• Downstream Processes
Organized by Jim Neville, Aaron Noyes and Todd Przybycien
• Biomolecular and Biophysical Processes
Organized by Joel Kaar, Ben Hackel and Hardeep Samra
• Biofuels
Organized by Michelle O’Malley
• QbD
Organized by Chikako Torigoe and Shailendra Singh
• Emerging Technologies
Organized by Greg Thurber, Jacob Latone and Ian Wheeldon
• Biosimilars
Organized by Krishnan Sampathkumar and Jill Myers
• Computational Biotechnology
Organized by David Roush and Jennifer Reed
• Poster Session
Organized by Andre Dumetz and Bryan Berger
We would especially like to acknowledge BIOT Program Support Administrator Brandi Rubino, without
whose exceptional work this meeting would not have been possible. In addition, we are grateful for
the guidance and support of BIOT Long-Range Program Coordinator Claire Komives.
We are also grateful for the assistance of ACS staff members who helped in planning the meeting,
including Dianne Ruddy, Laura Melohn and Robin Green.
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ACS BIOT - San Diego, California 2016
6
Awards Presentations &
Non-Technical BIOT Meetings
Tuesday, March 15, 2016
Biochemical Technology Division:
Events & Meetings
2016 Spring National Meeting
March 13 - 17, 2016
San Diego, CA
All locations in the Westin San Diego Hotel, unless otherwise noted
Sunday, March 13, 2016
David Perlman Memorial Lecture (Sponsored by: Bristol-Myers Squibb)
Ann Lee, “From Bench to Bedside: Developing Molecules into Therapies”
Emerald Ballroom, 11:30 AM
James M. Van Lanen Distinguished Service Award
Scott Banta,11:30 AM, Emerald Ballroom
Company Seminar - GE Healthcare Life Sciences
Henrik Ihre Ph.D., “Affinity chromatography – it is not only Protein A media”
Topaz, 12:30 PM
BIOT Young Investigator Award (Sponsored by: Genentech, Inc.)
Hal Alper, “Metabolic Engineering of Yarrowia lipolytica for fuels and chemicals production”
Emerald Ballroom, 11:30 AM.
W.H. Peterson Award Presentation
Best Oral Presentation
Stephanie Galanie, a graduate student from Professor Christina Smolke’s group at Stanford University for her
presentation “Synthetic biochemical production of medicinal protoberberine alkaloids in yeast”, at the 2015 ACS
meeting in Denver, Colorado.
11:30 AM, Emerald Ballroom
Best Poster Presentation
Jeremy Sauer, a graduate student from Prof. Ravi Kane’s and Jon Dordick’s groups at Rensselaer Polytechnic
Institute for his presentation titled “Remote activation of TRPV1 for therapeutic effect”, at the 2015 ACS meeting in
Denver, Colorado.
11:30 AM, Emerald Ballroom
Future Program/BIOT Business Meeting
Crystal I, 12:30 PM.
Merck, Inc. Keytruda CMC Team, “Commercialization of Keytruda: Overcoming Molecular”
Emerald Ballroom, 5:00 PM
BIOT Members Reception
USS Midway, 6:30-9:00 PM
Monday, March 14, 2016
Marvin J. Johnson Award Lecture (Sponsored by: Pfizer)
David Schaffer, “Directed Evolution of New Viruses for Therapeutic Gene Delivery”
Emerald Ballroom, 11:30 AM
Company Seminar - Bio-Rad
Jiali Liao, “Exploring the Unique Selectivity of Mixed-Mode Chromatography for Challenging Biomolecule
Separations”
Topaz, 12:30 PM
Sci-Mix
San Diego Convention Center Hall D 6-8 PM
Executive Committee Meeting ***By Invitation Only*** Topaz, 7:00 PM
BIOT Poster Session (Sponsored by: MilliporeSigma)
San Diego Convention Center Hall E, 6:00 PM
Awards Dinner : ***By Invitation Only***
Wednesday, March 16, 2016
Biotechnology & Bioengineering Daniel I.C. Wang Young Investigator Award
Fuzhong Zhang, “Synthetic pathway and control systems for overproduction of fatty acids and their derivatives”
Emerald Ballroom, 5:00 PM.
Program Chair’s Meeting: ***By Invitation Only***
Networking/Mentoring Session
Topaz, 6:00 PM.
Thursday, March 17, 2016
Biotechnology & Bioengineering Elmer Gaden Award Lecture
Jens Nielsen, “Yeast as a Platform Cell Factory for Production of Fuels and Chemicals”, Emerald Ballroom, 11:30
AM.
7
8
Westin San Diego
9
Hotel Maps
10
Continuous shuttle bus service between the Westin San Diego and
The USS Midway will be provided
Sponsored by:
State St.
Kettner Blvd.
USS
Midway
USS
Midway
Westin
San Diego
West E St.
Columbia St.
6:30 - 9:00 PM
Sunday March 13, 2016
West E St.
Pacific Hwy
910 N. Harbor Drive
San Diego
Harbor Ln.
N Harbor Dr.
W. Broadway
Columbia St.
The BIOT Members Reception will be held on the:
Directions to the USS Midway
India St.
BIOT Members Reception
West F St.
1. Head straight down W. Broadway, towards N.
Harbor Dr.
2. Turn Left onto N. Harbor Drive
BIOT membership is required for reception attendance.
3. Follow E. Harbor Drive all the way to the USS
Midway
Come by the registration table for your BIOT Dot!
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Keynote Speakers
Biosimilars
Jeffrey Baker
United States Food & Drug Administration
Biofuels
Adam Arkin
University of California, Berkeley
Multiple techniques for host and pathway engineering of tolerance and
chemical production.
Session: Synthetic Biology and - OMICS Approaches to Engineering Microbial Communities
Sunday, March 13th at 2:40 pm
Opal
Considerations in the development and control of biosimilars manufacturing in
the United States
Session: Development & Manufacturing Considerations for Biosimilars
Tuesday March 15th at 8:50 am
Crystal I
Upstream Processes
Huimin Zhao
University of Illinois, Urbana-Champaign
Biophysical & Biomolecular Processes
Jennifer Cochran
Stanford University
Building a biological foundry for next-generation synthetic biology
Session: Metabolic Engineering and Synthetic Biology: Tools Development
Tuesday, March 15th at 2:00 pm
Crystal II
New Approaches for high-throughput analysis and engineering of enzymes and
protein therapeutics
Session: Protein Engineering and Design I
Monday, March 14th at 9:10 am
Diamond I & II
Emerging Technologies
Eric Shusta
University of Wisconsin, Madison
Modeling the blood-brain barrier using human pluripotent stem cells
Computational Biotechnology
Bernhard Palsson
Session: Stem Cells and Regenerative Medicine
Tuesday, March 15th at 2:40 pm
Opal
University of California, San Diego
Genome-scale models can compute proteome allocation
Session: Bio-Design and Systems Analyses at the Cellular Scale
Monday, March 14th at 8:50 am
Crystal I
Quality by Design
John Erickson
GlaxoSmithKline
QbD six years after A-mAb: What we expected, what happened, and what now
Upstream Processes
Session: QbD Case Studies: Process Characterization
Wednesday, March 16th at 9:10 am
Crystal I
Sang Yup Lee
KAIST
Strategies for developing industrial microbial strains for chemicals production
Session: Microbial Processes Development
Monday, March 14th at 2:40 pm
Crystal II
Downstream Processes
Jill Myers
Momenta Pharmaceuticals
Downstream Processes: Past, present and future
Session: Technology, Transfer, Scale-up and Scale-Down
Wednesday, March 16th at 2:00 pm
Emerald Ballroom
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BIOT Division Awards 2016
Greetings Fellow BIOT Enthusiasts!
The BIOT Division of ACS celebrates the research and service achievements of seven BIOT awardees at
the San Diego national meeting, including the David Perlman Memorial Lectureship, the Marvin J. Johnson
Award in Microbial and Biochemical Technology, the Industrial Biotechnology Award, the Young Investigator
Award, the James M. Van Lanen Distinguished Service Award and the William H. Peterson Student Oral
and Poster Presentation Awards for presentations made at last year’s Denver meeting. Our distinguished
awardees are introduced in this booklet along with a more detailed description of the nature of each award, a
brief biographical sketch and the title of the awardee’s associated award lecture where applicable. The dates,
times and locations of the award presentations are also listed.
In addition to the BIOT Division awardees, we will recognize the San Diego meeting program organizers,
Peter Tessier of Rensselaer Polytechnic Institute and Scott Tobler of Merck. The Biotechnology and Bioengineering Elmer Gaden and Daniel I.C. Wang awards will also be conferred at the Denver meeting.
The BIOT Division thanks the sponsors of our awards, Bristol-Myers Squibb (Perlman Lecture), Pfizer (Johnson Award) and Genentech (Young Investigator Award), for their generous support. We also wish to thank
the selection committee members for each award for their service to the Division and the broader research
community as well as those who made the effort to nominate a deserving colleague in 2016.
Please consider nominating a colleague for a BIOT award in 2017 – award descriptions, nomination procedures and deadlines (typically the first of August each year) are provided at http://www.acsbiot.org/index.php/
awards.
David Perlman Memorial Lectureship
Sunday March 13th, 11:30 AM, Emerald Ballroom
“From Bench to Bedside: Developing Molecules into Therapies”
Ann Lee, Genentech, Inc.
This award, sponsored by Bristol-Myers Squibb, showcases compelling speakers with leading
research programs of special interest to the BIOT membership. This award recognizes Dr. Lee’s
contributions and leadership in the area of industrial bioprocess research and development.
Ann L. Lee is Senior Vice President, Genentech and Head of Global Technical Development at
Roche. Her organization is responsible for developing the processes, analytical methods, and CMC
strategies to deliver all of the new products in Roche’s pipeline, as well as technology transfers and
technical support for global commercial manufacturing operations. The organization encompasses
the full range of development functions: biologics, small molecules, devices, drug delivery, packaging,
analytical and QC, engineering, quality assurance, clinical supply chain management, project and portfolio management,
development contract manufacturing management, business excellence, as well as global manufacturing science and
technology. Prior to joining Genentech in 2005, Ann worked for 16 years at Merck, where she held several leadership
positions in vaccine development in the Merck Research Laboratories, and then served as Vice President, Chemical
Technology and Engineering in the manufacturing division. Lee holds a BSChE from Cornell, and a Ph.D. from Yale.
She has 39 scientific publications and numerous patents. Ann was elected to the National Academy of Engineering in
2007, and is a member of numerous professional societies. She serves on various academic and scientific advisory
boards including for vaccines at the Gates Foundation. She received the Corporate Innovation Award from the AIChE
on behalf of Genentech, was named as one of 150 most influential women in Bay Area Business, and was awarded
the Genentech Women Professionals’ Leadership Award. She serves as executive sponsor for various internal and
external organizations supporting leadership development, diversity, and mentoring boys and girls in science.
Congratulations to all of the 2016 awardees – see you in San Diego!
Best regards,
James M. Van Lanen Distinguished Service Award
Sunday March 13th, 11:30 AM, Emerald Ballroom
Scott Banta, Columbia University
Todd Przybycien
BIOT Awards Chair
This award is intended to recognize an individual for outstanding contributions to the ACS Division
of Biochemical Technology through committed and sustained service. Professor Banta is receiving
this award in recognition of his leadership and service in the BIOT Division including service as
Chair of the Division, meeting organization and meeting participation.
Scott Banta Ph.D. is a Professor of Chemical Engineering at Columbia University. He received his
B.S.E degree from the University of Maryland, Baltimore County, and his M.S. and Ph.D. degrees
from Rutgers University. He did a postdoctoral fellowship with Professor Martin Yarmush at the
Shriners and Massachusetts General Hospitals and Harvard Medical School. He began his faculty
career in the Department of Chemical Engineering at Columbia University in 2004 and his research
has focused on the engineering of proteins and peptides for various applications in areas including
biocatalysis, bioeletrocatalysis, biomaterials, gene and drug delivery, biosensing, and bioenergy. His group is also
developing new biotechnology platforms for energy harvesting and conversion.
Photo Credit: Eileen Barroso
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Marvin J. Johnson Award in Microbial and Biochemical Technology
Monday March 14th, 11:30 AM, Emerald Ballroom
“Directed Evolution of New Viruses for Therapeutic Gene Delivery”
David Schaffer, University of California, Berkeley
This award, sponsored by Pfizer, Inc., is given in recognition of outstanding research contributions
to microbial and biochemical technology. Prof. Schaffer is being recognized for his pioneering
development of directed evolution to optimize gene therapy delivery vehicles and for the engineering
of microenvironments for controlling step cell therapies.
David Schaffer is a Professor of Chemical and Biomolecular Engineering, Bioengineering, and
Neuroscience at University of California, Berkeley, where he also serves as the Director of the
Berkeley Stem Cell Center. He received a B.S. in Chemical Engineering from Stanford University in
1993 and a Ph.D. in Chemical Engineering from the Massachusetts Institute of Technology in 1998.
He then conducted a postdoctoral fellowship at the Salk Institute for Biological Studies in La Jolla,
CA before becoming a faculty member at the University of California at Berkeley in 1999. At Berkeley, Dr. Schaffer
applies engineering principles to enhance stem cell and gene therapy approaches for neuroregeneration, work that
includes novel approaches for molecular engineering and evolution of new viral vectors as well as new technologies to
investigate and control stem cell fate decisions. David Schaffer has received an NSF CAREER Award, Office of Naval
Research Young Investigator Award, Whitaker Foundation Young Investigator Award, and was named a Technology
Review Top 100 Innovator. He was also awarded the American Chemical Society Marvin Johnson Award in 2016, the
American Chemical Society BIOT Division Young Investigator Award in 2006, the Biomedical Engineering Society Rita
Shaffer Young Investigator Award in 2000, and was inducted into the College of Fellows of the American Institute of
Medical and Biological Engineering in 2010.
Tuesday March 15th, 11:30 AM, Emerald Ballroom
“Metabolic Engineering of Yarrowia lipolytica for fuels and chemicals production”
Hal Alper, University of Texas at Austin
This award, sponsored by Genentech in 2016, recognizes an outstanding young contributor to the
field of biochemical technology who is also an active participant in the BIOT Division programs.
Professor Alper is receiving this award for pioneering new approaches that merge and advance
the areas of synthetic biology, protein engineering, and metabolic engineering to achieve novel
phenotypes including chemical overproduction.
Dr. Hal Alper is an Associate Professor in Chemical Engineering at The University of Texas at Austin.
He earned his Ph.D. in Chemical Engineering from the Massachusetts Institute of Technology
in 2006 and was a postdoctoral research associate at the Whitehead Institute for Biomedical
Research (2006-2008) and at Shire Human Genetic Therapies (2007-2008). He is currently the
Principal Investigator of the Laboratory for Cellular and Metabolic Engineering where his lab focuses on metabolic
and cellular engineering in the context of biofuel, biochemical, and biopharmaceutical production in an array of model
host organisms. His research focuses on applying and extending the approaches of related fields such as synthetic
biology, systems biology, and protein engineering. He has published over 60 articles and 8 book chapters. Dr. Alper
is the recipient of the Camille and Henry Dreyfus New Faculty Award (2008), the Texas Exes Teaching Award (2009),
the DuPont Young Investigator Award (2010), the Office of Naval Research Young Investigator Award (2011), the UT
Regents’ Outstanding Teaching Award (2012), the 2013 Biotechnology and Bioengineering Daniel I.C. Wang Award,
the Jay Bailey Young Investigator Award in Metabolic Engineering (2014), the Camille Dreyfus Teacher-Scholar Award
(2014), and the SIMB Young Investigator Award (2015).
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W.H. Peterson Award
Tuesday March 15th, 11:30 AM, Emerald Ballroom
The W.H. Peterson Award is granted each year by the Division of Biochemical Technology to student members who
present outstanding research work in sessions sponsored by the division at ACS national meetings.
Best Oral Presentation
Stephanie Galanie, a graduate student from Professor Christina Smolke’s group at Stanford University, is being recognized for her presentation “Synthetic biochemical production of medicinal protoberberine alkaloids in yeast”, at the 2015 ACS meeting in Denver, Colorado.
Best Poster Presentation
Jeremy Sauer, a graduate student from Prof. Ravi Kane’s and Jon Dordick’s groups at Rensselaer
Polytechnic Institute, is being recognized for his presentation titled “Remote activation of TRPV1 for
therapeutic effect”, at the 2015 ACS meeting in Denver, Colorado.
Tuesday March 15th, 5:00 PM, Emerald Ballroom
Merck, Sharp, and Dohme, Inc., Keytruda CMC Team
Award Lecture by David Roush on behalf of the Keytruda CMC Team
(Team members : Stephen Farrand, Kevin Gurney, Thomas Linden, Chakravarthy Narasimhan, Kimberly May,Dennis
Rendeiro, Daisy Richardson, David Roush, Mohammed Shameem, Manoj Sharma, Nihal Tugcu)
This award recognizes the Keytruda team for its investment in the fundamental linkage of molecular design to activity,
stability, process and formulation which not only supported the rapid initial commercialization of Keytruda but also
enabled a robust process and formulation that could be deployed at multiple DS and DP manufacturing sites (via
analytical comparability) to support the drug product requirements for the large number (> 30) of indications.
Dr. Roush received his Bachelor of Science Degree in Chemical Engineering from Rice University and
completed his Ph.D. in Chemical Engineering at University of Houston under the guidance of Dr. Richard
Willson. Subsequently, David joined Merck Research Labs where his current title is Distinguished
Scientist, Process Development and Engineering, part of the Biologics Research area of Merck Research
Labs. He has been an active researcher in the purification of biologics (proteins, natural products and
vaccines) for 20 years, co-authoring more than 50 publications and presentations on the subject. Dr.
Roush has been engaged in the Biochemical Technology (BIOT) division of American Chemical Society
for more than 15 years. He currently serves as Chair Elect of the BIOT Division and BIOT Liaison to
Recovery of Biologics Conference Series.
18
Additional Awards to be Presented at the
San Diego Meeting
Company Seminars
Our generous sponsors will be providing a FREE LUNCH for
attending their seminars.
Biotechnology & Bioengineering Daniel I.C. Wang Young Investigator Award
Wednesday March 16th, 5:00 PM, Emerald Ballroom
“Synthetic pathway and control systems for overproduction of fatty acids and their derivatives”
Fuzhong Zhang, Washington University
The Biotechnology & Bioengineering Daniel I.C. Wang Award was initiated in 2008 to honor a
younger member of our dynamic community for his or her exceptional research contributions and
sustained commitment to the Journal.
Dr. Fuzhong Zhang is an assistant professor at the Department of Energy, Environmental and
Chemical Engineering, Washington University in St. Louis. He received his bachelor degree at
Peking University, Ph.D degree at University of Toronto and postdoctoral training at UC Berkeley/
Joint BioEnergy Institute. His current research focuses on developing synthetic biology tools and systems for the sustainable production of structurally-defined chemicals and high-performance materials. Dr. Zhang has received numerous young faculty awards, including the DARPA Young Faculty Award (2013), the ORAU Junior Faculty Enhancement
Award (2013), the NSF CAREER Award (2014), the AFOSR and ONR Young Investigator Program (2015), the HFSP
Young Investigators Award (2015), the NASA Early Career Faculty Award (2015), and the B&B Daniel I.C. Wang Award
(2016).
Sunday March 13
12:30 pm - 2:00 pm
Topaz
Affinity chromatography – it is not only Protein A media
Henrik Ihre, Ph.D., Director Custom Design Media, GE Healthcare Life Sciences
Biotechnology & Bioengineering Gaden Award Lecture
Thursday March 17th, 11:30 AM, Emerald Ballroom
“Yeast as a Platform Cell Factory for Production of Fuels and Chemicals”
•
•
•
Different Modes of Affinity chromatography
The evolution of affinity chromatography in downstream processing
What are the drivers for cost efficient affinity chromatography media?
Jens Nielsen, Chalmers University of Technology
In honor of its founding editor Elmer L. Gaden, Jr., Biotechnology and Bioengineering provides an
annual award for a high-impact paper reflecting exceptional innovation, creativity, and originality.
Jens Nielsen has an MSc degree in Chemical Engineering and a PhD degree (1989) in Biochemical
Engineering from the Danish Technical University (DTU), and after that established his independent
research group and was appointed full Professor there in 1998. He was Fulbright visiting professor
at MIT in 1995-1996. At DTU he founded and directed Center for Microbial Biotechnology. In 2008
he was recruited as Professor and Director to Chalmers University of Technology, Sweden, where he
is currently directing a research group of more than 50 people. At Chalmers he established the Area of Advance Life
Science Engineering, a cross departmental strategic research initiative and was founding Head of the Department
of Biology and Biological Engineering, which now encompass more than 170 people. Jens Nielsen has published
so far more than 550 papers that have been cited more than 18,000 times (current H-factor 67), co-authored more
than 40 books and he is inventor of more than 50 patents. He has founded several companies that have raised more
than M25EUR in venture capital. He has received numerous Danish and international awards including the Nature
Mentor Award, and is member of several academies, including the National Academy of Engineering in USA, the
Royal Swedish Academy of Science, the Royal Danish Academy of Science and Letters, the Royal Swedish Academy
of Engineering Sciences and the American Academy of Microbiology. He is a founding president of the International
Metabolic Engineering Society.
19
Henrik is a graduate from the Royal Institute of Technology in Stockholm, Sweden with
a Post Doc. from UC Berkeley, California, USA. Henrik joined GE Healthcare 2000 as
a Scientist, later becoming the Product Manager for GE Healthcare’s Protein A media
portfolio and later the Program Manager for the Antibody Purification program. Since
2009 Henrik holds the position at Director of GE Healthcare’s Custom Design Media
(CDM) program ensuring that more niche market needs and leads are explored and
brought to market.
For the last seven years Henrik has been a part of several custom affinity chromatography media
projects with customers worldwide resulting in a broad and solid knowledge around the key drivers
and features required for industrial downstream protein purification processes. In many ways the
CDM organization has been in the forefront of the next generation affinity resin development from
GE Healthcare with its early and detailed customer collaborations for new target molecules and
requirements around improved process economy and productivity which will be shared during the
presentation.
20
Company Seminars
Our generous sponsors will be providing a FREE LUNCH for
attending their seminars.
Special Programming on
This special Symposium, Computationally Enabled Biotechnology at the Molecular, Cellular and
Process Scales, is aligned with the primary theme of the 251st ACS National Meetings, Computers
in Chemistry. The research papers contributed to this Symposium highlight the interdisciplinary and
broad utilization of computational approaches to enable the creation of systems biology models and
to design therapeutic molecules and biologic production processes. One highlight of the Symposium
is a keynote lecture by Dr. Bernhard Palsson during the Bio-Design and Systems Analyses at the
Cellular Scale session.
Monday March 14
12:30 pm - 2:00 pm
Topaz
Exploring the Unique Selectivity of Mixed-Mode Chromatography for
Challenging Biomolecule Separations
• Interactions between protein molecules and mixed-mode resins
• Development of chromatographic methods
• Case studies on the removal of process- and product-related impurities
Jiali Liao is principal scientist of process chromatography R&D, Bio-Rad Laboratories.
He obtained a Ph.D in biochemistry in Uppsala University, Sweden in 1990 and
promoted to associate professor in 1995. From 1990 he has been working with BioRad for the development of Continuous-Bed Monolith Chromatography Columns
(UNOTM Column), UNOsphereTM and NuviaTM chromatographic resins. He authored
more than 40 papers and holds 12 US patents on both chromatography and capillary
electrophoresis (CE).
Computationally Enabled Biotechnology at the Molecular,
Cellular and Process Scales
Symposium organizers:
David Roush, Merck, Sharp and Dohme
Jennifer Reed, University of Wisconsin, Madison
Session: Biomolecular Design and Biophysics
Sunday March 13, 2-5 pm (Crystal I)
Session chairs: Howard Salis (Pennsylvania State University) and David Roush (Merck, Sharp and
Dohme)
Session: Bio-Design and Systems Analyses at the Cellular Scale
Monday March 14, 8:30-11:30 am (Crystal I)
Session chairs: Anthony P. Burgard (Genomatica) and Jeffrey Varner (Purdue University)
Session: Process Modeling in Biotechnology
Monday March 14, 2-5 pm (Crystal I)
Session chairs: Ajoy Velayudhan (University College London) and Robert Todd (KBI Biopharma)
21
22
The Best of BIOT Awards
This award recognizes the best presentations from the BIOT division at the National Meeting. We
are happy to acknowledge the 2015 Best of BIOT award winners.
Shear contributions to cell culture performance and product recovery in ATF and TFF perfusion systems
Samantha Wang, Boehringer Ingelheim
Disruptive Bioprocessing
Panel Discussion
Industrial and Academic Perspectives on Upstream and
Production of bispecific antibodies in “Knobs-into-Holes” using a cell-free expression system
Yiren (Joy) Xu, Sutro Biopharma
Sunday March 13, 5-6 pm (Emerald Ballroom)
“Exploring options for continuous diafiltration”
Matthew Westoby, Biogen
Mammalian manufacturing processes have become increasingly productive and scalable for a wide
variety of therapeutic proteins. However, cost and timeline pressures require process development
scientists and researchers – both industrial and academic – to find new, disruptive ways to more
efficiently manufacture therapeutic proteins without compromising consistency or product quality.
Capacity assessment: DBC is not the whole story – implications for resin lifetime and sequential
multicolumn chromatography
Justin Ladwig, Amgen
Laser extraction from micropore arrays enables high-throughput enzyme engineering
Arvind Kannan, Stanford University
Fitness landscapes as comprehensive measures of adaptive tradeoffs in the engineering of protein function
Barrett Steinberg, Johns Hopkins University
High-throughput passive microrheology of therapeutic protein solutions
Lilian Lam Josephson, University of Delaware
This theme will be explored throughout the day on Sunday, including a technical session in Upstream
Processing on Sunday morning, a technical session in Downstream Processing on Sunday
afternoon, and a Panel Discussion on Sunday evening. In the evening event, a panel of experts
in both the upstream and downstream fields will discuss their views on topics including continuous
bioprocessing, single-use technologies, -omics technologies, and various other disruptive tools to
accelerate process development and deepen product knowledge. The discussion will consist of 20
minutes of introductory slides by panelists followed by a 40 minute Q&A session open to the general
audience.
Cell penetrating peptides for stem cell applications
Won H. Suh, Temple University
Effective strategy for a comprehensive QbD characterization of Viresolve® filter: A collaboration between
MedImmune and EMD millipore
Gisela Ferreira, MedImmune
Challenges and approaches in demonstrating biosimilarity at the physicochemical and biological level
Joseph L. Glajch, Momenta Pharmaceuticals
Building important molecules from methanol
Igor Bogorad, UCLA
Organizers: Varnika Roy (MedImmune) & Todd Przybycien (Carnegie Mellon University)
Disruptive Bioprocessing Upstream Session Chairs: Michael Betenbaugh (Johns Hopkins University) and Albert
Schmelzer (MedImmune)
Sunday March 13, 8:30 - 11:30 am (Crystal I)
Disruptive Bioprocessing Downstream Session Chairs: John Pieracci (Biogen) and Kevin Brower (Genzyme)
Sunday March 13, 2-5 pm (Emerald Ballroom)
Upstream & Downstream Panelists:
As part of their award, each winner presented their award winning talk as part of the Best of BIOT webinar
series.
And now you can play a role in selecting the
2016 Best of BIOT content!
Cast your vote by giving five stars to your favorite presentations within the ACS BIOT conference app.
Kelvin Lee, Professor of Chemical and Biomolecular Engineering (University of Delaware)
Rohini Deshpande, Executive Director, Process Development (Amgen)
Sadettin Ozturk, Senior Vice President, Process & Analytical Development (Mass Biologics)
Engin Ayturk, Senior R&D Manager, BioPharm Applications R&D (Pall)
Steven Cramer, Professor of Chemical and Biological Engineering (RPI)
Jon Coffman, Director, BioProcess Engineering (Boehringer Ingelheim)
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24
Sunday Morning
A
B
C
D
25
**
**
**
Upstream Processes
Area Coordinators: M. Antoniewicz, M. Blenner, V. Roy
Papers 1-8
8:30-11:30 AM
Crystal I
Presiding: M. Betenbaugh, A. Schmelzer
Vaccines, Gene Therapy Vectors and
Non-Protein Biological Products
Papers 9 - 16
8:30-11:30 AM
Emerald Ballroom
Area Coordinators: J. Neville, A. Noyes, T. Przybycien
Presiding: C. Heldt, J. Stout
Biomolecular & Biophysical
Processes
Papers 17 - 24
8:30-11:30 AM
Diamond I
Imaging, Sensing, and Bioactuation
Area Coordinators: J. Kaar, B. Hackel, H. Samra
Presiding:G. Thurber, T. Wellman
Biofuels
Synthetic Biology Approaches to Engineer
Production of Fuels and Energy Molecules
Papers 25 - 32
8:30-11:30 AM
Opal
Area Coordinators: M. A. O’Malley
Presiding: T. Moon, C. Eckert
Perlman Memorial Lectureship,
(Sponsored by Bristol-Myers Squibb)
11:30 AM-12:30 PM
Emerald Ballroom
Van Lanen & Peterson Award Presentations
Company Lunch Seminar: GE HealthCare
I&EC: ACS Award in Separations Science & Technology:
Symposium in honor of Steven M. Cramer
12:30 - 2:00 PM
Topaz
8:30-11:30 AM
Temecula 3&4 - Marriott Marquis San Diego Marina
26
8:30 - 11:30AM, Crystal I
Presiding: M. Betenbaugh, A. Schmelzer
Sponsored by: Northwestern Biotech MS Program
BIOT 1 - 8:30
Understanding and mitigating raw material variability in an
intensified, integrated perfusion cell culture process.
M. Hollenbach, J. Wang, N. Shah, J. Walther, C. Hwang
BIOT 2 - 8:50
Debottlenecking
manufacturing
capacity:
Initiating
cell culture manufacturing campaigns using seed train
cryopreserved in a disposable bag.
S. Rameez, S. Gopalakrishnan, K. Zhang, S.S. Mostafa, A.A.
Shukla
BIOT 3 - 9:10
Single-use membrane bioreactor for culturing therapeutic
T-cells.
S. Yoo, J. Bramson, R. Ghosh
BIOT 4 - 9:30
Development of a CHO cell-free synthetic platform for
production of monoclonal antibodies.
N. Majewska, R. Martin, K. Moore, A. Schmelzer, M. Jewett, V.
Roy
9:50 - Intermission
BIOT 5 - 10:10
Continuous processing for pre-clinical and clinical manufacturing.
J.L. Coffman
BIOT 6 - 10:30
Development of a high performance integrated and disposable
clarification solution for continuous bioprocessing.
M. Collins, E. Ayturk, R. Gantier
BIOT 7 - 10:50
Acoustic wave separation, an alternative cell clarification
technology: Optimization and applications.
J. Armando, J.P. Pieracci, P. Haberman, J. Rozembersky, C.
Leidel, J. King, D. Bianchi
BIOT 8 - 11:10
Glycoengineering in CHO cells for biomanufacturing.
Q. Wang
27
Vaccines, Gene Therapy Vectors and
Non-Protein Biological Products
8:30 - 11:30AM, Emerald Ballroom
Presiding: C. Heldt, J. Stout
BIOT 9 - 8:30
Leveraging design of experiments to characterize the effects of
formaldehyde concentration, incubation temperature and time
on influenza A/WS/33 (H1N1) inactivation and process analytics.
S. Patel, L. Mullin, M. Bruce, K. Cunningham, J. Caron, C.
Gillespie
BIOT 10 - 8:50
Development of a cGMP-compatible purification process for
adenovirus purification.
M.A. Snyder
BIOT 11 - 9:10
Residual DNA removal by Benzonase® endonuclease in
influenza feedstreams.
L. Mullin, S. Patel, M. Bruce, K. Cunningham, J. Caron, C.
Gillespie
BIOT 12 - 9:30
Viral particle purification with osmolyte flocculation.
C. Heldt, A. Saksule
9:50 Intermission
BIOT 13 - 10:10
Use of preconditioning to control membrane fouling and enhance
performance during ultrafiltration of plasmid DNA.
Y. Li, A.L. Zydney
BIOT 14 - 10:30
Aggressive development of two recombinant protein production
processes to generate a bivalent vaccine.
R. Krishnan, A. Berrill, B. Huffman, J. Cundy, S. Cook, W.
Wellborn, J. Martin, K. Sunasara
BIOT 15 - 10:50
Upstream and downstream solutions for MSCs animal originfree processing.
L. Savary, A. Schnitzler, D. Kehoe, M. Aysola, A. Verma, T.
Lawson, S. Rigby, T. Hood, S. Punreddy, S. Luther, J. Murrell,
M. Rook, M. Pease, M. Bulpin
BIOT 16 - 11:10
Ultrafiltration for purification of polysaccharide-based vaccines.
M. Hadidi, J.J. Buckley, A.L. Zydney
Biomol. & Biophysical Processes
Imaging, Sensing & Bioactuation
8:30 - 11:30AM, Diamond I
Presiding: G. Thurber, T. Wellman
BIOT 17 - 8:30
PET imaging of growth factor receptor expression in cancer
using the 45-amino acid Gp2 scaffold.
M. Kruziki, E. Zudock, B. Case, B. Hackel
BIOT 18 - 8:50
Binary aptamer for instant fluorescent analysis of specific
nucleic acids in vitro.
N. Kikuchi, D. Kolpashchikov
Biofuels
Synthetic Biology Approaches to Engineer
Production of Fuels & Energy Molecules
8:30 - 11:30AM, Opal
Presiding: C. Eckert, T. Moon
BIOT 25 - 8:30
GPCR-based chemical biosensor for advanced biofuels.
P. Peralta Yahya
BIOT 26 - 8:50
Rapid construction of metabolite biosensors using domain
insertion profiling.
D. Savage
BIOT 19 - 9:10
Imaging the oxidation state of peroxiredoxin-2 in living cells.
T.F. Langford, H. Sikes
BIOT 27 - 9:10
Synthetic extracellular sensing circuit by intein-mediated
reconstitution of yeast mating factor.
K. Siu, W. Chen
BIOT - 9:30
Development of transient induced molecular electronic
spectroscopy (TIMES) for protein-ligand interactions. T. Zhang,
H. Ma, I. Lian, T. Wei, Y. Lo
BIOT 28 - 9:30
Deep sequencing-guided assessment and computational
design of synthetic metabolic pathways.
J. Klesmith, T. Whitehead
9:50 Intermission
9:50 Intermission
BIOT 21 - 10:10
Engineering a novel diagnostic test for tuberculosis using
nanoparticle-based detection of a whole blood gene expression
signature.
H. Gliddon, P.D. Howes, E. Kim, M. Kaforou, M. Levin, M.
Stevens
BIOT 29 - 10:10
PIACE: Parallel integration and chromosomal expansion of
biofuels pathways in E. coli.
G. Goyal, J. Alonso Guitierrez, J.D. Keasling, T. Lee, N. Hillson
BIOT 22 - 10:30
Improving the durability of graphene composite sensors for
robust biosensors.
S. Tuntithavornwat, J. King, C. Heldt
BIOT 23 - 10:50
Micromachined multielectrode microprobes for choline sensing
with an on-probe iridium oxide reference.
L. Feng, H. Monbouquette
BIOT 24 - 11:10
Directed evolution of yeast peptide receptors: A new platform for
low-cost healthcare diagnostic.
A. Adeniran, S. Stainbrook, K.E. Tyo
Sunday Morning
Sunday Morning
Upstream Processes
BIOT 30 - 10:30
Synthetic regulon in Saccharomyces cerevisiae for efficient
xylose assimilation.
V. Endalur Gopinarayanan, N. U. Nair
BIOT 31 - 10:50
Engineering anaerobic methanogenic pathway and methylcoenzyme M reductase in Methanosarcina acetivorans. M.
Raeeszadeh Sarmazdeh, J. Gonzalez, W. Chen
BIOT 32 - 11:10
Novel gut fungal transporters for improved fuel and energy
production.
K. Solomon, J. Henske, S.P. Gilmore, S. Seppala, M. Rite, M.A.
O’Malley
28
Upstream Processes
Mammalian Cell Culture Process Development I
Papers 34 - 41
2:00 - 5:00 PM
Crystall II
Presiding: N. Lewis, B. Mulukutla
Disruptive BioProcessing
Papers 42 - 49
2:00 - 5:00 PM
Emerald Ballroom
Upstream Processes
Mammalian Cell Culture Process Development I
2:00 - 5:00PM, Crystall II
Presiding: N. Lewis, B. Mulukutla
Sponsored by: Genentech
BIOT 34 - 2:00
Identification and optimization of small molecule inhibitors of
epigenetic modifying enzymes for enhancing transient protein
production in mammalian cells.
M. Christensen, K. Rege
BIOT 35 - 2:20
Enabling use of sugar-based detergents as a shear protectant
in CHO cell culture medium.
J. Lakkyreddy, J. Wuu, S. Meier
Presiding: K. Brower, J. Pieracci
Processes
Papers 50 - 57
2:00 - 5:00 PM
Diamond I
Controlling the Interface of Proteins, Cells and
Materials
BIOT 36 - 2:40
Fine tuning and coarse tuning of protein glycosylation profiles
through novel media supplementation strategies.
C. Racicot, S. McDermott, J. Matuck, C. Chumsae, P. Hossler
BIOT 37 - 3:00
High-throughput microbioreactors for CHO cell transfection
process development.
C. Hsu, H. Jain, J. Wang, K.A. Brorson
Disruptive BioProcessing
2:00 - 5:00PM, Emerald Ballroom
Presiding: K. Brower, J. Pieracci
Sponsored by: Pall Life Sciences
Sunday Afternoon
A
B
C
Sunday Afternoon
BIOT 42 - 2:00
Investigation of single pass tangential flow filtration as an inline
concentration step during cell culture harvest.
M. Peck, A. Arunkumar, N. Singh
BIOT 43 - 2:20
Withdrawn
BIOT 44 - 2:40
Technical considerations for an affinity resin discovery and
development platform to rapidly address complex separations
of diverse therapeutics and vaccines.
W. Kett
BIOT 45 - 3:00
Split intein-mediated self-cleaving tag for recombinant protein
purification in a mammalian cell expression system.
T. Han, D.W. Wood
3:20 Intermission
Area Coordinators: B. Hackel, J. Kaar, H. Samra
D
E
**
29
3:20 Intermission
Presiding: J. Kaar, M. Kastantin
Biofuels
Synthetic Biology and -OMICS Approaches to
Engineer Microbial Communities
Papers 58 - 64
2:00 - 5:00 PM
Opal
Presiding: K. Solomon, H. Tseng
Biomolecular Design and Biophysics
Papers 65 - 72
2:00 - 5:00 PM
Crystal I
Area Coordinators: J. Reed, D. Roush
Presiding: H. Salis, D. Roush
Disruptive BioProcessing: Panel Discussion
5:00 PM
Emerald Ballroom
BIOT 38 - 3:40
Influenza vaccine production using cell culture with
microcarriers.
M. McGlothlen, L. Mullin, P. Hatch, D. Asher, C. Gillespie
BIOT 39 - 4:00
Case study on the production of live virus vaccine on platforms
supporting anchorage dependent cell substrate.
D. Spatafore, S. Christanti, S. Fox, J.C. Gercke, C.D. Jan, K.
Hamaker
BIOT 40 - 4:20
Development of a high-productivity cell culture process and
implementation at large scale.
E. Ward, W. Yang, A. Doane, W. Hu
BIOT 46 - 3:40
Enabling continuous low pH viral inactivation with integrated inline conditioning of protein A streams.
M. Fiadeiro, J.A. Kublbleck, R. Fahrner, J. Salm
BIOT 47 - 4:00
Optimized continuous mAb polishing via coupled unit
operations.
X. Gjoka, K. Rogler, M. Bisschops, R. Gantier, M. Schofield
BIOT 48 - 4:20
Efficient devices for high-resolution membrane chromatography.
R. Ghosh, P. Madadkar, R. Sadavarte
BIOT 49 - 4:40
PD scale realization of an integrated continuous bioprocessing
platform.
E. Ayturk, R. Gantier
BIOT 41 - 4:40
Development run case study for a monoclonal antibody NS0
cell culture process.
E. Hodgman, S. Agastin, C. Lucini, J. Shiminsky, I. Pla
30
Sunday Afternoon
Controlling the Interface of Proteins, Cells &
Materials
2:00 - 5:00PM, Diamond I
Presiding: J. Kaar, M. Kastantin
Synthetic Biology & - OMICS Approaches to
Engineering Microbial Communities
2:00 - 5:00PM, Opal
Presiding: K. Solomon, H. Tseng
Biomolecular Design & Biophysics
2:00 - 5:00PM, Crystal I
Presiding: H. Salis
Sponsored by: Novo Nordisk
BIOT 50 - 2:00
Spheroid and tissue assembly via click chemistry in microfluidic
flow.
M.N. Yousaf
BIOT 58 - 2:00
Engineering recombinant microbial communities for cellulose
degradation.
K.Z. Kalbarczyk, M. Koffas, C.H. Collins
BIOT 65 - 2:00
Discovery and design of novel regulatory noncoding RNA in
bacteria.
J. Marcus, S. Hassoun, N.U. Nair
BIOT 51 - 2:20
Plasma membrane imaging and cancer therapy based on cell
surface engineering.
F. Wu, H. Wang, H. Jia, X. Hua, Y. Li, J. Sun, Z. Chen
BIOT 59 - 2:20
Bottom-up construction of synthetic microbial pairs inspired by
nature.
S. Gilmore, J.A. Sexton, J. Henske, K. Solomon, M.K.
Theodorou, M.A. O’Malley
BIOT 66 - 2:20
Understanding and exploiting enzyme promiscuity for metabolic
engineering.
D. Pertusi, J. Jeffryes, K.E. Tyo
BIOT 52 - 2:40
Dense poly(ethylene glycol) brushes reduce protein adsorption
and promote unfolding.
D. Marruecos, M. Kastantin, D.K. Schwartz, J. Kaar
BIOT 53 - 3:00
Withdrawn
3:20 Intermission
BIOT 54 - 3:40
Modification of bicelles with peptoids to alter edge chemistry.
H. Najafi, S.L. Servoss
BIOT 55 - 4:00
Dopa-Fe3+ complexation of mussel adhesive proteins at plaquesubstrate interface.
B. Yang, C. Lim, B. Choi, D. Hwang, H.J. Cha
BIOT 56 - 4:20
Characterization of protein-functionalized surfaces created by
sortase-mediated ligation.
S. Williamson, L. Parks, R. Le, M. Raeeszadeh Sarmazdeh, P.
Frymier, E.T. Boder
BIOT 57 - 4:40
Adsorption and electron transfer of deca-heme cytochrome
(MtrF) studied with atomistic simulations and kinetic Monte
Carlo simulation.
H. Ma, M. Sajib, T. Wei
31
Biofuels
Keynote: BIOT 60 - 2:40
Multiplex techniques for host and pathway engineering of
tolerance and chemical production.
A.P. Arkin
3:20 Intermission
BIOT 61 - 3:40
Genome-scale engineering in probiotic organisms.
T. Mansell, S. Rothstein
BIOT 62 - 4:00
Optimization of E. coli co-cultures for the high-yield production
of plant polyphenols.
J.A. Jones, V.R. Vernacchio, D.M. Lachance, S.M. Collins,
A.L. Sinkoe, J. Hahn, M. Koffas
BIOT 63 - 4:20
Experimental examination of catabolite repression and diauxic
growth in Clostridium phytofermentans.
H.W. Harris, A. Navid, Y. Jiao, B. Stewart
BIOT 64 - 4:40
Engineering a synthetic sugar sensing yeast strain.
K.M. Blocker, A.S. Robinson
Disruptive Bioprocessing Panel
Discussion
5:00 PM Emerald Ballroom
Sponsored by: MedImmune
Sunday Afternoon
5:00 PM -Panel Discussion
BIOT 67 - 2:40
Development and validation of computational methods for de
novo design of antibody variable regions.
T. Li, V. Poosarla, T.K. Wood, C. Maranas
BIOT 68 - 3:00
Suppressed inactivation of α-amylase in the presence of
alcohol.
A. Khan
3:20 Intermission
BIOT 69 - 3:40
Molecular origins of high viscosity in solutions of monoclonal
antibody mutants.
A. Vaish, D.J. Rosenman, S. Yadav, I. Zarraga, A.M. Lenhoff
BIOT 70 - 4:00
Modulation of protein biophysical properties with small molecule
in purification and formulation.
F.K. Insaidoo, H. Li, N. Tugcu, D.J. Roush
BIOT 71 - 4:20
Binding of proteins to multimodal surfaces: From fundamental
molecular modeling to prediction of chromatographic retention.
S. Banerjee, S. Parimal, K. Srinivasan, S. Garde, S.M. Cramer
BIOT 72 - 4:40
Biopharmaceutical informatics: Selection of well-behaved
molecules with predictive algorithms for enhanced process and
product development of biologic drugs.
S. Kumar, D. Tomar, E. Stephens, A. Tiwari, J.C. Rouse, S.K.
Singh
32
E
F
**
**
33
Upstream Processes
Mammalian Cell Culture Process Development II
Papers 73 - 80
8:30-11:30 AM
Crystal II
Presiding: P. Apostolidis, C. Metallo
Advances in Chromatographic Separations I
Papers 81 - 88
8:30-11:30 AM
Emerald Ballroom
Presiding: S. Cramer, J. Pollard
Biomol. & Biophys. Processes
Protein Engineering and Design I
Papers 89 - 95
8:30-11:30 AM
Diamond I & II
Presiding: A. Karlsson, D. Liu
Biofuels
Engineering Microbes to Utilize Next Generation
Feedstocks
Papers 96 - 103
8:30-11:30 AM
Opal
Presiding: P. Peralta-Yahaya, T. Lee
Bio-Design and Systems Analyses at the Cellular
Scale
Mammalian Cell Culture Process Development II
8:30 - 11:30AM, Crystal II
Presiding: P. Apostolidis, C. Metallo
BIOT 73 - 8:30
Distinct metabolic states support pluripotent stem cell selfrenewal.
M. Badur, H. Zhang, A. Divakaruni, S. Parker, C. Jaeger, K.
Hiller, A. Murphy, C. Metallo
BIOT 74 - 8:50
3M purification solution for mycoplasma retention.
D.V. Smirnov, N. Stepanova, G.M. Jellum
BIOT 75 - 9:10
Transcriptome analysis of Chinese hamster ovary (CHO) cell
lines under low temperature conditions using RNA-seq.
Y. Gowtham, C. Saski, S.W. Harcum
BIOT 76 - 9:30
Process intensification through integration of upstream perfusion
cell culture with downstream continuous chromatography in
monoclonal antibody production. .
A. Castan, T. Falkman, E. Fäldt, L. Blomqvist, A. Forss
9:50 Intermission
Upstream Processes
Papers 104 - 110
8:30-11:30 AM
Crystal I
Area Coordinators: J. Reed, D. J. Roush
BIOT 77 - 10:10
Increasing diversity of production cell lines through
miniaturization, automation, and high-throughput analytics.
K. Le, H. Victor, K. Daris, T. Munro
BIOT 78 - 10:30
Automatic bioreactor feeding: Moving forward with metabolic
activity monitoring for nutrient feeding.
G. Emmerson, S. Watts, G. Barringer
Presiding: A. Burgard, J. Varner
Biocatalysis and Biotransformations
Papers 111 - 118
8:30-11:30 AM
Pearl
Area Coordinators: J. Kaar, B. Hackel, H. Sharma
Presiding: K. Brower, P. Cirino
Marvin J. Johnson Award Lecture
11:30 AM-12:30 PM
Emerald Ballroom
Company Lunch Seminar: Bio-Rad
12:30 - 2:00 PM
Topaz
(Sponsored by Pfizer, Inc.)
BIOT 79 - 10:50
Using a CFD model to better understand and optimize cell
culture performance using the ambr15™ reactor. W.J. Kelly, X.
Li, Z. Huang, E.J. Schaefer, S. Subramanian
BIOT 80 - 11:10
Cell death during production of biologics and its consequences
on release of the intracellular contents.
M. Krajcovic, S. Hutchins, K. Aron, M.C. Borys, Z. Li
Advances in Chromatographic Separations
Sponsored by: Janssen Research &
Development
BIOT 81 - 8:30
Evaluation of novel adsorptive hybrid filters for impurity removal
during biologics purification.
N. Singh, A. Arunkumar, M. Peck, A. Voloshin, J.F. Hester
BIOT 82 - 8:50
Maximizing productivity with AEX membrane adsorbers: Impact
on robustness and edge of failure.
W. Rayfield, D.J. Roush, A. Gospodarek, M. Brower, C. Cutler,
N. Tugcu
BIOT 83 - 9:10
Evaluation of ligand design for downstream processes:
Application to aggregate removal.
J.K. Rasmussen, C. Bothof, G. Griesbraber, A. Vail, S. Colak
Atan, F. Sgolastra
Monday Morning
A
B
C
D
Monday Morning
BIOT 84 - 9:30
Developing high performance responsive HIC membrane
adsorbers.
Z. Liu, X. Qian, R. Wickramasinghe
9:50 Intermission
BIOT 85 - 10:10
Breaking through the productivity barrier: Integration of a novel
modular chromatography scaffold and a new resin design to
achieve a hyper-productive Protein A capture process.
M. Siwak, G. de los Reyes, R. Todd
BIOT 86 - 10:30
Insight into the profile of associated HCP with protein A resin
cycle number.
K. Lintern, M. Pathak, A.S. Rathore, D.G. Bracewell
BIOT 87 - 10:50
Evaluation of excipients and cleaning solutions to enhance the
performance of Protein A media.
.A. Becerra-Arteaga, A. Gupta
BIOT 88 - 11:10
Best of both worlds: High affinity and structural stability in
3D-structured synthetic ligands for protein purification by affinity
chromatography.
S. Menegatti
34
Protein Engineering & Design
8:30 - 11:30AM, Diamond I & II
Monday Morning
BIOT 89 - 8:30
Systemic depletion of serum L-cyst(e)ine with an engineered
human enzyme mediates potent induction of ROS and
specifically sabotages tumor metabolism.
S. Cramer, A. Saha, S. Tadi, S. Tiziani, W. Yan, K. Triplett, S.
Alters, D.E. Johnson, Y. Zhang, J. DiGiovanni, G. Georgiou, E.
Stone
BIOT 90 - 8:50
Lessons learned from using phage display to select for fibrin
binding peptides.
J. Rice
New approaches for high-throughput analysis and engineering
of enzymes and protein therapeutics.
J. Cochran
9:50 Intermission
BIOT 92 - 10:10
Computational redesign of acyl-ACP thioesterase for mediumchain fatty acid production.
M. Grisewood, N. Hernandez-Lozada, B. Pfleger, C. Maranas
BIOT 93 - 10:30
Engineering substrate specificity of chymotrypsin for mass
spectrometry based proteomics.
S. Abnouf, B. Ramesh, N. Varadarajan
BIOT 94 - 10:50
Peptide ligands bind to electrostatic domains of Abeta.
A. Murray, J. Lippens, D. Fabris, G. Belfort
BIOT 95 - 11:10
Inclusion of inter-domain linkers improves the stability and
function of fusion proteins in cell-based assays.
J. Cook, A. Charlesworth
35
Biofuels
Engineering Microbes to Utilize Next Generation
Feedstocks
8:30 - 11:30AM, Opal
Presiding: T. Lee, P. Peralta - Yahya
BIOT 96 - 8:30
Unveiling cryptic xylose metabolism in Yarrowia lipolytica.
G.M. Rodriguez, M. Shabbir-Hussain, L. Gambill, M.A. Blenner
BIOT 97 - 8:50
Enzyme assembly for increased methanol utilization.
J.V. Price, L. Chen, W. Chen
BIOT 98 - 9:10
Conversion of lignin-derived aromatic compounds into lipids by
engineered Rhodococcus opacus strains. W.R. Henson, S. Kim,
Y.J. Tang, M. Foston, G. Dantas, T. Moon
BIOT 99 - 9:30
Optimal pathway rebalancing for 3-hydroxypropionic acid
production from glycerol in Escherichia coli.
H. Lim, M. Noh, G. Jung
9:50 Intermission
Bio-Design & Systems Analyses at the Cellular
Scale
Presiding: A. Burgard, J. Varner
Sponsored by: Merck and Co., Inc.
BIOT 111 - 8:30
Influence of the intracellular metabolite profile on outcomes in
protein engineering.
C. Cooper
BIOT 105 - 9:10
Development and analysis of precursor production strains for
chemical production.
X. Zhang, C. Tervo, J. Reed
BIOT 112 - 8:50
Role of redundancy in metabolic networks and its implications in
metabolic engineering.
E.C. Brunk, J. Monk, A. Sastry, B. Palsson
BIOT 106 - 9:30
Detection of novel metabolites and enzyme functions through in
silico expansion of metabolic models.
J. Jeffryes, C. Lerma-Ortiz, A.J. Cooper, T. Niehaus, A. Thamm,
O. Frelin, T. Kind, L.J. Broadbelt, O. Fiehn, A. Hanson, K.E. Tyo,
C. Henry
BIOT 113 - 9:10
Designing custom subcellular organelles in bacteria.
B. Jakobson, M. Slininger, E. Kim, J. Glasgow, M. Asensio, Y.
Chen, D.T. Ercek
9:50 Intermission
BIOT 107 - 10:10
iSCHRUNK: In silico approach to characterization and reduction
of uncertainty in the kinetic models of genome-scale metabolic
networks.
S. Andreozzi, L. Miskovic, V. Hatzimanikatis
BIOT 101 - 10:30
Synthetic methylotrophy: Engineering Escherichia coli to
metabolize methanol for growth and metabolite production.
W.B. Whitaker, R.K. Bennett, M. Palmer, J. Gonzalez, M.R.
Antoniewicz, E.T. Papoutsakis
BIOT 108 - 10:30
System-level analysis of phototrophic metabolism of aromatic
compounds in Rhodopseudomonas palustris. A. Navid, Y. Jiao,
J. Pett-Ridge
BIOT 103 - 11:10
Engineering Ralstonia eutropha to produce fuels and chemicals
from diverse biomass-derived substrates.
S. Singer
Biocatalysis & Biotransformations
8:30 - 11:30AM, Pearl
Presiding: K. Brower, P. Cirino
Genome-scale models can compute proteome allocation.
B.O. Palsson
BIOT 100 - 10:10
Comprehensive bioconversion of algae to liquid fuels and
intermediate value products.
R.W. Davis, W. Wu, M. Tran-Gyamfi, T. Lane, R. Pate, B. Wu
BIOT 102 - 10:50
Deciphering the regulation of biomass degradation by anaerobic
fungi.
J. Henske, K. Solomon, M.K. Theodorou, I. Grigoriev, M.A.
O’Malley
BIOT 109 - 10:50
Modeling of cell-free glycoprotein production in Escherichia coli.
N. Horvath, J. Wayman, M.C. Jewett, M.P. DeLisa, J. Varner
BIOT 110 - 11:10
Enabling selection in directed evolution of enzymes via cellular
engineering.
N. Hassanpour, E. Ullah, M. Yousofshahi, N.U. Nair, S. Hassoun
Monday Morning
BIOT 114 - 9:30
HaloTag mediated artificial cellulosome assembly on DNA
template for efficient cellulose hydrolysis.
Q. Sun, W. Chen
9:50 Intermission
BIOT 115 - 10:10
Biosynthesis of high-value inositol via in vitro synthetic enzymatic
pathways.
Y. Zhang, C. You
BIOT 116 - 10:30
Understanding short and medium chain
biosynthesis by AATase activity in yeast.
J. Zhu, J. Lin, I.R. Wheeldon
volatile
ester
BIOT 117 - 10:50
Discovery, expression and characterization of novel
extremozymes from the deep Red-Sea brine pools.
S. Groetzinger, E. Strillinger, A. Frank, M. Groll, D. WeusterBotz, J. Eppinger
BIOT 118 - 11:10
Extreme thermoacidophiles as biocatalysts for heavy metal
recovery: A delicate balance between biooxidation and
resistance.
G. Wheaton, A. Mukherjee, J. Counts, J. Kruh, B. Ijeomah, J.
Desai, R.M. Kelly
36
B
C
Upstream Processes
Papers 120 - 126
2:00 - 5:00 PM
Crystal II
E
37
2:00 - 5:00PM, Crystal II
Presiding: M. Jewett, J. Latone
Advances in Chromatographic Separations
Sponsored by: MilliporeSigma
Presiding: M. C. Jewett, J. Latone
Strategies for developing industrial microbial strains for
chemicals production.
S. Lee
BIOT 127 - 2:00
High capacity polishing
purification processes.
Y. Tao
BIOT 121 - 2:40
Strain engineering for robust E. coli fermentation processes.
K. Veeravalli, T. Schindler, M. Yamada, R. Hamilton, K. Bodner,
M. Laird
BIOT 128 - 2:20
High throughput determination of cleaning solutions to prevent
the fouling of an anion exchange resin.
T. Elich, T. Iskra, W. Daniels, C.J. Morrison
BIOT 122 - 3:00
Metabolic engineering with a dual-acting small RNA molecule
for improved biofuels fermentations.
A. Lahiry, S.D. Stimple, R. Lease, D.W. Wood
BIOT 129 - 2:40
Method development for resin selectivity screening in
RoboColumn® format.
A. Kiesewetter, P. Menstell, L. Peeck, A. Stein
3:20 Intermission
BIOT 130 - 3:00
Variable surface transport modalities on functionalized
chromatographic supports revealed with single molecule
spectroscopy.
L.J. Tauzin, H. Shen, J.K. Rasmussen, C. Bothof, G.
Griesbraber, A.K. McNulty, C.F. Landes
Advances in Chromatographic Separations II
Area Coordinators: J. Neville, A. Noyes, T. M. Przybycien
Papers 127 - 134
2:00 - 5:00 PM
Emerald Ballroom
Processes
Papers 135 - 142
2:00 - 5:00 PM
Diamond I & II
Protein Engineering and Design II
D
Upstream Processes
Biofuels
Biomass Pretreatment and Hydrolysis
Papers 143 - 150
2:00 - 5:00 PM
Opal
Presiding: M. Foston, M. Resch
Process Modeling in Biotechnology
Area Coordinators: D. Roush, J. Reed
Presiding: R. Todd, A. Velayudhan
Papers 151 - 158
2:00 - 5:00 PM
Crystal I
BIOT 123 - 3:40
Rapid production and characterization of membrane-bound
oligosaccharyltransferases with the aid of cell-free protein
synthesis.
J. Schoborg, J. Hershewe, J. Techner, M. Mrksich, M. Jewett
BIOT 124 - 4:00
Bioprocess development of a natural product: An integrated
strain improvement and fermentation process development
program to accelerate process improvement.
M.R. Mikola, N. Amrhein, S. Becker, P. Bhosale, E. Blackburn,
D. Brown, J. Brunson, S. Casada, M. Chase, N. Clark, U. Galm,
R. Garrison, K. Hill, E. Ibwe, H. Jones, J. Jones, P. Ketterer, P.
Lewer, A. Lutocka, P. Maddipati, L. Marcun, J. Marty, E. Miller,
E. Miller, N. Mouncey, N. Pollack, B. Raman, T. Ramseier, P.
Reifel, R. Roberts, M. Roach, T. Schatzer, P. Speakman, C.
Stowers, E. Traub, D. Tyagi, B. Ward, K. Work, S. Wensing
BIOT 125 - 4:20
Cooperation in a synthetic microbial community through
engineered commensalism.
E.E. Kelly, C.H. Collins
BIOT 126 - 4:40
Dynamic two-stage fermentation in S. cerevisiae allows for rapid
and scalable strain design for microbial chemical synthesis.
B. Reed, M. Lynch, J. Burg
chromatography
for
antibody
Monday Afternoon
A
Monday Afternoon
3:20 Intermission
BIOT 131 - 3:40
Probing nanoscale effects of ligand density in tentacular ion
exchangers using x-ray scattering.
R.S. Bhambure, D.G. Greene, C. Gillespie, M.W. Phillips, H.
Graalfs, A. Rapp, A.M. Lenhoff
BIOT 132 - 4:00
Application of mechanistic modeling for enabling PAT in process
chromatography: Separation of charge variants of mAbs by ion
exchange chromatography.
V. Kumar, A.S. Rathore
BIOT 133 - 4:20
Spectral deconvolution of chromatograms with PLS models
and calibration-free methods.
M. Rüdt, N. Brestrich, J. Hubbuch
BIOT 134 - 4:40
General gradients for preparative chromatography.
A. Holmqvist, A. Sellberg, F. Magnusson, B. Nilsson, A. Staby
38
Protein Engineering & Design II
2:00 - 5:00PM, Diamond I & II
BIOT 135 - 2:00
When bad is good: Directed evolution using negative selection
can result in alleles with superior properties. B. Steinberg, M.
Ostermeier
BIOT 136 - 2:20
Creating catenanes with lasso peptides.
C.D. Allen, A. Link
Monday Afternoon
BIOT 137 - 2:40
Yeast surface display-based method for the directed evolution
of optimized self-cleaving intein purification tags.
S.D. Stimple, M.J. Coolbaugh, Y. Fan, K. Cochran, D.W. Wood
BIOT 138 - 3:00
Computationally de novo designed peptide-mediated heterooligomeric interactions for modular self-assembly of protein
and lipoprotein nanostructures.
M.S. Ardejani
3:20 Intermission
BIOT 139 - 3:40
Functional selection of full-length antibodies in the cytoplasm of
living bacterial cells.
M. Robinson, N. Ke, M. Berkemen, M.P. DeLisa
BIOT 140 - 4:00
Improved methods for co-evolving the affinity and stability of
antibody fragments specific for amyloid-forming polypeptides.
K.E. Tiller, M. Julian, C. Lee, L. Rabia, J. Young, P.M. Tessier
BIOT 141 - 4:20
Engineering antibody specificity through multi-dimensional
high-throughput screens.
A. Li, Y. Cho
BIOT 142 - 4:40
Evolving an intrinsically disordered peptide, the β-roll, for
biomolecular recognition.
B. Bulutoglu, K. Dooley, S. Banta
39
Biofuels
Biomass Pretreatment & Hydrolysis
2:00 - 5:00PM, Opal
Presiding: M. Foston, M. Resch
BIOT 143 - 2:00
Multimodal characterization of industrial steam exploded
biomass samples: A modelling approach to predict enzymatic
digestibility.
T. Auxenfans, B. Chabbert, G. Paes
BIOT 144 - 2:20
Evaluation of In planta transient expression of cell wall degrading
enzymes as a biological pretreatment for cellulosic biomass.
L. Anthony, M. Hwang, M. Phu, B.W. Falk, A.M. Dandekar, K.
McDonald
BIOT 145 - 2:40
Energy analysis of algal biocrude production.
E. Martinez-Guerra, V. Gude
Process Modeling in Biotechnology
Presiding: R. Todd, A. Velayudhan
Sponsored by: KBI BIopharma
BIOT 151 - 2:00
Using a Monte Carlo simulation model to predict process
performance during scale-up.
R. Ashton, A. Gates, J. Moscariello
BIOT 152 - 2:20
Advanced multiscale metabolic modeling of a nitrogen fixing
cyanobacterium.
J. Gardner, B. Miller, W. Sinclair, H. North, B. Hodge, N.R.
Boyle
BIOT 153 - 2:40
Bioprocess modeling of fouling phenomena in cross-flow
microfiltration of viable bacteria.
X. Li, S. Ku, K. Thomas, K. Foster, E. Ximenes, H. Jaycey, X.
Liu, M.R. Ladisch
BIOT 146 - 3:00
Enhanced saccharification of biomass to aqueous soluble
oligosaccharides and monosaccharides at high biomass
loading in molten salt hydrate medium.
N. Li, J. Kraft, X. Pan
BIOT 154 - 3:00
Facilitating the analysis of complex samples using data
reduction techniques: Case studies in downstream processing.
S. Konstantinidis, N. Field, K. Jurlewicz, A.K. Velayudhan
3:20 Intermission
3:20 Intermission
BIOT 147 - 3:40
Effect of water-soluble kraft lignin fraction on the enzymatic
saccharification of pretreated poplar.
Y. Jin, Y. Wang, W. Wang
BIOT 155 - 3:40
Designing flow-through chromatography processes for
aggregate removal.
S. Hasegawa, D. Itoh, Y. Isakari, N. Yoshimoto, A. Podgornik,
S. Yamamoto
BIOT 148 - 4:00 Liquid hot water pretreatment inhibitors.
E. Ximenes, Y. Kim, C. Farinas, M.R. Ladisch
BIOT 149 - 4:20
Determination of lignin kinetics during organosolv pretreatment.
J. Meyer, M.B. Foston
BIOT 150 - 4:40
Making room during pretreatment.
A. Ragauskas, Y. Pu, M. Li, C.G. Yoo
Monday Afternoon
BIOT 156 - 4:00
Mechanistic modeling of chromatography and its application
in biopharmaceutical process development. B. Stueckler, K.
Doninger, J. Griesbach
BIOT 157 - 4:20
General gradients for preparative chromatography: Mechanistic
modeling and practical consideration.
A. Sellberg, A. Holmqvist, C. Andersson, B. Nilsson
BIOT 158 - 4:40
Ensuring long term robustness of a CEX chromatographic step
for separation of charge variants with optimized yield.
K. Haeringer, E. Rosenberg, S. Hepbildikler, K. Lacki, E.
Brekkan, M. Ahnfelt
40
B
C
D
E
Engineering Non-Model Hosts for Biological
Production
Papers 159 - 166
8:30-11:30 AM
Diamond II
Presiding: H. Dhamankar, D. T. Ercek
Antibodies, Drug Conjugates and Related
Molecules I
Papers 167 - 174
8:30-11:30 AM
Emerald Ballroom
Presiding: K. Mehta, N. Sanaie
Protein Therapeutics: Discovery and Production
Papers 175 - 182
8:30-11:30 AM
Diamond I
Presiding: Y. Chen, N. Basse
New Tools and Approaches I
Area Coordinators: G. Thurber, J. Latone, I. Wheeldon
Papers 183 - 190
8:30-11:30 AM
Opal
Presiding: B. Throndset, J. Wang
Biosimilars
Development and Manufacturing Considerations
for Biosimilars
Papers 191 - 195
8:30-11:30 AM
Crystal I
Area Coordinators: J. Myers, K. Sampathkumar
Upstream Processes
Metabolic Engineering and Synthetic Biology:
Pathways/Products
Papers 196 - 203
8:30-11:30 AM
Crystal II
**
Engineering Non-Model Hosts for Biological
Production
8:30 - 11:30AM, Diamond II
Presiding: H. Dhamankar, D. T. Ercek
BIOT 159 - 8:30
Novel gut fungal sugar transporters for improved bioprocess
efficiency.
S. Seppala, K. Solomon, J. Henske, S.P. Gilmore, M.D. Rieth,
M.A. O’Malley
BIOT 160 - 8:50
Metabolic engineering of the marine cyanobacterium,
Synechococcus sp. PCC 7002 for phototrophic production of
L-lysine and sugar feedstocks.
A.L. Markley, B. Pfleger
BIOT 161 - 9:10
Extreme thermophile metabolic engineering platforms:
Production of biofuels and bio-based chemicals at elevated
temperatures.
A.J. Loder, B.M. Zeldes, A. Hawkins, C.T. Straub, M.W. Keller,
G.L. Lipscomb, G.J. Schut, M.W. Adams, R.M. Kelly
BIOT 162 - 9:30
Clostridium autoethanogenum, a chassis for low carbon fuel
and commodities production at commercial scale by gas
fermentation.
S. Nagaraju
9:50 Intermission
Presiding: O. Jaquez, K. Sampathkumar
F
41
Upstream Processes
Upstream Processes
BIOT 163 - 10:10
Yarrowia lipolytica as a technology platform for industrial
applications.
Q.Q. Zhu
BIOT 164 - 10:30
High efficiency genome editing in Yarrowia lipolytica by CRISPR/
Cas9.
C.M. Schwartz, M. Shabbir-Hussain, B. Simmons-Rawls, A.
Loebs, M.A. Blenner, I.R. Wheeldon
BIOT 165 - 10:50
Rapid isolation of chromosome hubs to facilitate nonconventional
yeast engineering.
Z. Shao, M. Cao, M. Gao, C. Lopez, Y. Wu
Presiding: C. Collins, H. Le
(Sponsored by Genentech, Inc.)
11:30 AM-12:30 PM
Emerald Ballroom
Molecules II
Presiding: J. Neville, A. Noyes, T. M. Przybycien
BIOT 167 - 8:30
Withdrawn
BIOT 168 - 8:50
Mitigating novel ADC impurity challenges through creative
approaches: Check the antibody variants.
X. Lin, J. Franklin, M. Hutchinson, J. Gorrell
BIOT 169 - 9:10
Selective on-column PEGylation of a therapeutic protein using
hydroxyapatite and fluoroapatite adsorbents.
M. Bakhshayeshi, G. Bolton, J.P. Pieracci, D. Cecchini
BIOT 170 - 9:30
Development of peptide based affinity media for the purification
of monoclonal antibodies: Effect of spacer arms, peptide variants
and process conditions.
T. Islam, A. Naik, Y. Hashimoto, S. Menegatti, R.G. Carbonell
9:50 Intermission
Tuesday Morning
A
Tuesday Morning
BIOT 171 - 10:10
Evaluating novel harvest methods for high density mammalian
cell culture processes.
M. Rohani, C. Chase, B. Guzman, K. Mehta, N. Osei-Owusu
BIOT 172 - 10:30
Evaluation of preparative ion exchange and multimodal
chromatography for separation of charge variants in the mAb
purification process.
A.Gronberg, T. Bjorkman, L. Kärf, A. Ljunglöf, E. Heldin
BIOT 173 - 10:50
Eco-friendly detergent for viral inactivation.
A. Ladiwala, S. Fisher, L. Norling, K. Abadie, G. Magill, N.
Magarian, K. Skidmore, R. Shearer, M. Butler, J. Gorrell, Q.
Chen, P. Lester
BIOT 174 - 11:10
Development of challenging aggregate removal step in a bispecific antibody purification process: A case study.
S. Jain, M. Mercaldi
BIOT 166 - 11:10
Programmable genetic sensors and circuits for pathway
engineering. A. Hoynes-O’Connor, C. Immethun, D. DeLorenzo,
K. Ng, T. Moon
42
Protein Therapeutics: Discovery & Production
Presiding: N. Basse, Y. Chen
BIOT 175 - 8:30
Selection of inhibitory antibodies using next generation high
throughput sequencing.
T.J. Lopez, X. Ge
BIOT 176 - 8:50
High-throughput conformational epitope mapping by deep
sequencing for antibody discovery.
T. Whitehead
Tuesday Morning
BIOT 177 - 9:10
Rethinking affibody: Evolving small proteins using data-driven
diversification.
D.R. Woldring, P.V. Holec, B. Hackel
New Tools & Approaches I
8:30 - 11:30AM, Opal
Presiding: B. Throndset, J. Wang
BIOT 183 - 8:30
Site-specific covalent labeling of RNA with RNA-TAG: A robust
technology applicable to a variety of biologically relevant
investigations.
S.C. Alexander, N.K. Devaraj
BIOT 184 - 8:50
New synthetic chemical biology approach for the development
of HIV-1 vaccine.
N. Wang, Z. Yuan, W. Niu, Q. Li, J. Guo
BIOT 185 - 9:10
Mimicking protein functions with entropically constrained
peptides.
A. Farrow, A. Wang, K. Deyle, D. Bunck, J.R. Heath
BIOT 178 - 9:30
Human G protein-coupled receptors expression and signaling in
yeast: Design and optimization of the host/protein platform for
therapeutic development.
.A. Jain, A.S. Robinson
BIOT 186 - 9:30
Peptoid-based microsphere coating to increase the binding
efficiency in sandwich ELISA microarrays.
G.R. Perez-Bakovic, S.L. Servoss
9:50 Intermission
9:50 Intermission
BIOT 179 - 10:10
Bispecific antibody vs. binary mixture: Synergy in the treatment
of pertussis.
A. Wagner, J. Maynard
BIOT 187 - 10:10
Disease screening pill for breast cancer: In vivo demonstration
of an orally available near-infrared molecular imaging agent
using mouse xenografts.
S. Bhatnagar, K. Dhingra, W. Kelley, J. Liao, A. Priluck, G.M.
Thurber
BIOT 180 - 10:30
Discovery of highly soluble antibodies prior to purification using
affinity-capture self-interaction nanoparticle spectroscopy.
J. Wu, J.S. Schultz, C.L. Weldon, S.V. Sule, Q. Chai, S.B. Geng,
C.D. Dickinson, P.M. Tessier
BIOT 181 - 10:50
Withdrawn
BIOT 182 - 11:10
Effect of air-liquid interface in monoclonal antibody aggregate
formation.
T.A. Mammo, B. Constantine, M. Zhu, D. Robbins
43
BIOT 188 - 10:30
High-throughput production of multifunctional suspension
microarrays by massive coding of dissociated elements.
J. Wang
BIOT 189 - 10:50
Bio-orthogonal chemistry enables proteomic analysis of
Staphylococcus aureus during mammalian infection.
S. Stone, D.A. Tirrell
BIOT 190 - 11:10
Engineering commensal microbes to fight against pathogen.
I. Hwang, M. Chang
Biosimilars
Development & Manufacturing Considerations for
Biosimilars
Presiding: O. Jaquez, K. Sampathkumar
Sponsored By: Momenta Pharmaceuticals, Inc.
8:30 Introductory Remarks
Considerations in the development and control of biosimilar
manufacturing in the United States.
J.C. Baker
BIOT 192 - 9:30
From process characterization to control strategy.
D. Boeth
9:50 Intermission
BIOT 193 - 10:10
Development of a biosimilar: Pooling efforts from all technical
forces.
R.S. Gronke, O. Jaquez, B. Woppmann, Y. Lyubarskaya, S.
Prajapati
BIOT 194 - 10:30
Flexibility in high-throughput two column purification platforms
for biosimilar mAb manufacturing.
K. Blando, C.W. Richey, G. Winzeler, K. Dhanasekharan, V.
Vinci
Upstream Processes
Metabolic Engineering & Synthetic Biology:
Pathways/Products
Presiding: C. Collins, H. Le
Sponsored by: Pfizer
BIOT 196 - 8:30
Exploring enhancement of 1,4-butanediol production in
recombinant E. coli using large-scale kinetic models.
S. Andreozzi, A. Chakrabarti, K. Soh, A.P. Burgard, T. Yang, S.
Vandien, L. Miskovic, V. Hatzimanikatis
BIOT 197 - 8:50
Metabolic biology of Saccharophagus degradans, a cellulosic
biomass-digesting bacterium, as revealed by 13C metabolic flux
analysis.
A. Quinn, B.D. Gastfriend, J.G. Corckran, S.W. Hutcheson, G.
Sriram
BIOT 198 9:10
Isopentenyl diphosphate (IPP)-bypass mevalonate pathways
for C5 alcohol production.
T. Lee
BIOT 199 - 9:30
Using phosphoproteomic analysis to better understand gene
regulatory networks.
M. Marten, N. Ramsubramaniam, C. Chelius, L. Ribeiro, K.
Boppidi, S. Li, S. Harris, R. Srivastava
Tuesday Morning
9:50 Intermission
BIOT 195 - 10:50
FMEA based characterization of biosimilar drug product
manufacturing.
S. Kamat, B. Bernat, J. TerWee
BIOT 200 - 10:10
Pathway manipulations enhance acetyl-CoA supply for natural
product synthesis.
J. Cardenas, N.A. Da Silva
11:10 Panel Discussion
BIOT 201 - 10:30
Complete biosynthesis of opioids in yeast.
S. Galanie, K. Thodey, I.J. Trenchard, M. Filsinger Interrante,
C.D. Smolke
BIOT 202 - 10:50
Construction of inducible gene expression systems in Pichia
pastoris for recombinant protein production.
J. Cao, P. Perez-Pinera, T. Lu
BIOT 203 - 11:10
Guiding CHO cell engineering for biopharmaceutical development
using genome-scale models of CHO cell metabolism.
N.E. Lewis
44
B
C
D
E
**
45
Upstream Processes
Tools Development
Papers 205 - 211
2:00 - 5:00 PM
Crystal II
Upstream Processes
Tools Development
Presiding: M. Lipscomb, K. Tyo
Antibodies, Drug Conjugates &
Related Molecules II
Presiding: K. Mehta, N. Sanaie
BIOT 205 - 2:00
Building a biological foundry for next-generation synthetic
biology.
H. Zhao
Presiding: M. Lipscomb, K. Tyo
Molecules II
Papers 212 - 219
2:00 - 5:00 PM
Emerald Ballroom
Presiding: N. Sanaie, K. Mehta
Protein Therapeutics: Formulation and Delivery
Papers 220 - 227
2:00 - 5:00 PM
Diamond I
Presiding: E. Chi, H. Samra
Stem Cells and Regenerative Medicine
Area Coordinators: G. Thurber, J. Latone, I. Wheeldon
Papers 228 - 234
2:00 - 5:00 PM
Opal
Presiding: J. Kim, P. Apostolidis
Biosimilars
Challenges in Demonstrating Analytical
Biosimilarity
Papers 235 - 239
2:00 - 5:00 PM
Crystal I
Area Coordinators: K. Sampathkumar, J. Myers
Presiding: B. Bernat, A. Fotopoulos
for
the
BIOT 207 - 3:00
Next generation synthetic biology tools for rapid, highthroughput optimization of metabolic pathways.
S. Chandran
Processes
BIOT 206 - 2:40
Development of platform-based technologies
optimization of sustainably produced chemicals.
S.J. Culler
5:00 - 6:00 PM
Emerald Ballroom
3:20 Intermission
BIOT 208 - 3:40
redGEM: A unbiased approach for systematic reduction of
genome-scale models.
V. Hatzimanikatis
BIOT 209 - 4:00
Co-opting uncharacterized CRISPR-Cas systems as the next
generation of biomolecular tools.
R. Leenay, K. Maksimchuk, C. Beisel
BIOT 210 - 4:20
Trackable genome engineering with single nucleotide
resolution.
A.D. Garst, M. Bassalo, G. Pines, S. Lynch, A. Edwards, R.T.
Gill
BIOT 211 - 4:40
Transcription activator-like effectors as tools for manipulation of
bacterial gene expression.
M.C. Politz, M. Copeland, B. Pfleger
BIOT 212 - 2:00
Development
of
efficient
cation
exchange
(CEX)
chromatography to remove low and high molecular weight
species for commercial manufacturing of a mAb.
M. Mayani, D. Yu, E. Schutsky, A.T. Lewandowski, Y. Song, S.
Traylor, R. Swanson, Z. Li
BIOT 213 - 2:20
Re-oxidization of partially reduced monoclonal antibody by
charged depth filters.
H. Hoang
BIOT 214 - 2:40
Natrix HD-Sb membrane adsorber: A new high-performance
purification tool for mAb purification in flowthrough mode.
D.M. Kanani, N. Paghdal, A. Shang, R. Jacquemart, J.G. Stout
BIOT 215 - 3:00
Influence of downstream processing on the glycosylation
of recombinant anthrax receptor fusion protein transiently
expressed in Nicotiana benthamiana.
K. Karuppanan, J.K. Muchena, S. Duhra-Gill, M. Phu, C.
Lebrilla, A.M. Dandekar, S. Nandi, K. McDonald
Tuesday Afternoon
A
Tuesday Afternoon
3:20 Intermission
BIOT 216 - 3:40
Enabling high-throughput downstream process development:
From method screening to process characterization.
J. Gervais
BIOT 217 - 4:00
Optimization of mAb charge and size variants separation using
dual pH/salt gradient elution IEC.
Y.F. Lee, S. Kluters, F. Wittkopp, C. Frech
BIOT 218 - 4:20
Evaluation of cation exchange chromatography resin lot
variability and optimization of process conditions for the
intermediate purification of a fusion protein.
A. Slocum, K. Sterl, R. Wright
BIOT 219 - 4:40
Knob-hole assembly of bispecific antibodies from halfantibodies.
A.J. Williams
46
Protein Therapeutics: Formulation & Delivery
Presiding: E. Chi, H. Samra
Stem Cells & Regenerative Medicine
2:00 - 5:00PM, Opal
Presiding: P. Apostolidis, Y. Kim
BIOT 220 - 2:00
Characterization of highly concentrated mAb solution: One step
towards the prediction of long-term stability.
M. Schermeyer, A.K. Wöll, J. Hubbuch
BIOT 228 - 2:00
CD264: A cell-surface marker for an aging population of
mesenchymal stem cells.
S. Madsen, K. Russell, A. Tucker, B. Bunnell, K. OConnor
BIOT 221 - 2:20
Predicting high-concentration antibody interactions
coarse-grained molecular modeling.
C. Calero-Rubio, R. Ghosh, A. Saluja, C.J. Roberts
BIOT 229 - 2:20
Regenerative medical bioadhesive based on photo-curable
mussel adhesive protein hydrogel.
E. Jeon, B. Choi, B. Hwang, Y. Yang, G. Jung, H.J. Cha
with
BIOT 222 - 2:40
Evaluation of pH-responsive hydrogel networks as oral delivery
systems for hematologic factor IX.
S. Horava, K. Moy, J. Liou, N. Peppas
Modeling the blood-brain barrier using human pluripotent stem
cells.
E. Shusta
BIOT 223 - 3:00
How predictive are protein-protein interactions of elevated
viscosity for protein solutions.
M.A. Woldeyes, C. Calero-Rubio, E.M. Furst, C.J. Roberts
3:20 Intermission
Tuesday Afternoon
3:20 Intermission
BIOT 224 - 3:40
Protein-excipient interaction hotspots: In
development and Fab case study.
T.S. Barata, P. Dalby, S. Brocchini, M. Zloh
silico
method
BIOT 231 - 3:40
Cell-derived microparticles for cell & gene therapy: The case of
megakaryocytic microparticles.
J. Jiang, C. Kao, E.T. Papoutsakis
BIOT 232 - 4:00
High yield in vitro expansion of cancer stem cells / tumor
initiating cells.
S.G. Tilson, E.M. Haley, Y. Kim
BIOT 225 - 4:00
Electrospun nanofibers of gelatin/cyclodextrin and their
potential application as hydrophobic drug delivery system.
A. Laha, S. Majumdar, C. Sharma
BIOT 233 - 4:20
Scalable hepatic differentiation of human endodermal cells
from pluripotent stem cell.
D. Chau, K. Ortiz, W. Hu
BIOT 226 - 4:20
Effects of small molecule co-solutes on the viscosity and stability
of highly concentrated solutions of monoclonal antibodies.
B. Dear, J. Hung, A. Borwankar, T. Truskett, K.P. Johnston
BIOT 234 - 4:40
Extracellular matrix mimetic chemical and physical cues for
promoting human mesenchymal stem cell tenogenic gene
expression.
M. Rehmann, A.M. Kloxin
Biosimilars
Challenges in Demonstrating Analytical
Biosimilarity
Presiding: B. Bernat, A. Fotopoulos
2:00 Introductory Remarks
BIOT 235 - 2:20
Characterization and quantitative comparison of remicade and
its biosimilar remsima.
A. Schwendeman
BIOT 236 - 2:40
Using critical quality attributes in the development of biosimilars.
B. Bernat, J. TerWee
BIOT 237 - 3:00
Higher order structure analysis using hydrogen deuterium
exchange mass spectrometry (HDX MS) and differential
scanning calorimetry (DSC).
C.F. Quinn
3:20 Intermission
Tuesday Afternoon
BIOT 238 - 3:40
Application biophysical techniques and NMR for higher order
structure biosimilarity studies.
J. Qiao, D. Tsao, R. Sridharan, J. Myers, C. Bell, J. Anderson
BIOT 239 - 4:00
Challenges and approaches in demonstrating biosimilarity at
the physicochemical and biological level.
J. Myers, V. Farutin, J. Glajch, J. Anderson
BIOT 227 - 4:40
Accelerating pharmaceutical development: Case studies with
automated approaches and formulation ‘miniaturization’.
R. Rajan, V. Razinkov, C. Ren, P. Yeh
47
48
BIOT – 242
Efficiency in cellbanking and seed preparation through
intensified perfusion.
A. Castan, O. Larsson
BIOT Poster Session
Organizers: Andre Dumetz, GSK
Bryan Berger, Lehigh University
BIOT - 243
Single-pass tangential flow filtration (SPTFF) for process
intermediate volume reduction.
N.E. Levy, A.C. Szkodny, T.H. Wiley, J.R. Molek, K.E. Goklen
BIOT – 244
Implementation of miniaturized fed-batch processes
in microliter-scale vessels for the development of high
performance CHO cell culture media.
M.R. Good, M. Goldfeld, W. Ling
BIOT – 245
Evaluating the effect of cross-flow pattern on product sieving
within hollow fiber cell retention devices in CHO cell perfusion.
P. McInnis, D. Rank, A. Dupont, C. Martin, M.W. Phillips
Light refreshments will be served.
6:00-9:00 PM
San Diego Convention Center
Hall E
Sponsored by: MilliporeSigma
49
BIOT – 246
Application of ambr250 mini-bioreactor platform to microcarrier
cultures for high throughput vaccine development.
W. Malik
BIOT – 247
Feed optimization to improve productivity and product quality
enabled by automated sampling system.
L. Hoshan, S. Xu, K. O’Neill, A. Mehta, T. Seamans, H. Chen
BIOT – 248
Characterizing oxygen kLa in bioreactors to support scale-up
and scale-down of cell culture bioprocesses.
G. Yao, S.R. Patel, Z. Ahmed, L. Marquardt, J. Lagos, K. Aron,
J. Yee, M.C. Borys, Z. Li
BIOT – 249
Gamma irradiated microcarrier-based virus production in a
single-use rocking bioreactor system.
A. Magnusson, M. Lundgren, E. Blanck, M. Berg
BIOT - 252
Evaluating the cytotoxicity of antioxidant and plasticizer
additives used in single use cell culture bags.
R. Shah, T. Linville, L. Yordy, J. Briggs, N. Chandarana, A.
Whynot, C.S. Brazel
BIOT – 253
Development of a model at home medical device using a twostep enzymatic process for the detection of hemoglobin A1c
levels in human blood.
N. Ledra, T. McCaffrey, J. Cabrera
BIOT – 254
Effect of surfactants on the fluorescence behavior of dyeconjugated DNA for FRET enhancement.
T. Oh, J. Choi, M. Heller
BIOT – 255
Synthesis of CdSe/ZnS nanocrystals and their bioconjugation
to DNA.
C.D. Hanson, L. Mohror
BIOT – 256
Simultaneous detection of Vibrio cholerae and cholera toxin
using a hybrid chip ba double biomolecular markers.
H. Shin, J. Seo, C. Kim, B. Hwang, H.J. Cha
BIOT - 257
Creating a DNA thermometer: An application for DNA
computation.
R.J. Karadeema
BIOT – 258
Micromachined multielectrode microprobes for glutamate and
dopamine detection.
A. Yorita, H. Monbouquette
Tuesday Evening
Tuesday Evening
BIOT – 241
New approaches to the chemical synthesis and characterization
of azetidinone.
E.J. Parish, Y. Lo, H. Honda, T. Wei, H. Shyu
BIOT – 259
Multiplexed detection of pathogenic DNA using zinc finger
proteins without DNA amplification.
J. Kim, M. Kim
BIOT - 260
Novel applications to the new development of amperometric
biosensor for serum cholesterol level determination.
Y. Lo, E.J. Parish, H. Honda, T. Wei
BIOT – 250
Implementing automated sampling, process monitoring, and
nutrient feedback control for a system of 3-L bioreactors.
K. O’Neill, A. Mehta, L. Hoshan, S. Xu, T. Seamans
BIOT – 261
Withdrawn
BIOT – 251
Withdrawn
BIOT – 262
Novel application of scanning electron microscopes and the
comparison of non-native Tephritid flies.
E.J. Parish, Y. Lo, H. Honda, T. Wei
50
BIOT – 274
Development of advanced genome engineering tools for a
model cyanobacterium.
H.R. Aucoin, M. Sanktjohanser, N.R. Boyle
BIOT – 285
Scale-down model development for a 5000-L production
bioreactor process using 0.5-L shake flask and 5-L bioreactor
systems. J. Discenza, H. Graham, E. Crabbe, N.G. Dalal
BIOT – 264
Withdrawn
BIOT – 275
Functional chitosan-nanofibers fabricated by an acidic shell
matrix protein for the preparation of efficient tissue engineering
scaffold. W. Song, Y. Choi
BIOT – 286
Hydrogenolysis of biomass-derived glycerol to propanediols
over Pd-Re catalyst: Influence of different Pd precursors.
Y. Li, L. Ma, D. He
BIOT – 276
New approaches to optically transparent elastic materials for
optical device in ophthalmology.
Y. Lo, H. Shyu, H. Honda, T. Wei
BIOT – 287
Optimizing metabolic pathways for the improved production of
natural products.
J.A. Jones, M. Koffas
BIOT – 277
Particle size evaluation for improved CHO and E. coli harvest
operations.
S. Gerepka, J. Bill, A. Naim, J. Borrajo
BIOT – 288
Improving scale-down models through characterization of
metabolism in a streptomyces fermentation.
J. Easson, S. Moses, M. Fieger
BIOT – 278
Dendronized polymers for mRNA delivery.
N.J. Oldenhuis, Z. Guan
BIOT – 289
Substrate-dependent surface display of a cellulosome by
Saccharomyces cerevisiae.
P. Botero Besada-Lombana, N.A. Da Silva
BIOT – 265
Strategies for optimizing a cell culture platform to achieve high
recombinant protein titer.
R. Gruver, S.B. Varma, N. Vijayasankaran, S.J. Meier
BIOT – 266
Improving the dynamic control of fatty acid ethyl ester
production.
A. Yaguchi, E. Arvay, G.M. Rodriguez, N. Wilson, M.A. Blenner
BIOT – 267
Gamma-aminobutyric acid production through GABA shunt
by the introduction of synthetic scaffolds in recombinant
Escherichia coli. S. Hong, V. Pham, S. Somasundaram
BIOT – 268
Genetic incorporation of unnatural amino acids biosynthesized
from α-keto acids by an aminotransferase.
W. Ko, H. Lee
BIOT – 269
Genetic incorporation of recycled unnatural amino acids. H.
Lee, S. Kim
Tuesday Evening
BIOT – 270
Translating unnatural amino acids with phenotypically-diverse,
engineered EF-Tu and tRNA variants.
V. Cox, E. Gaucher
BIOT – 271
Developing a protein-specific purification approach for
osteopontin expressed in chloroplast of Chlamydomonas
reinhardtii.
A. Ravi, S. Guo, S. Kulkarni, B. Rasala, M. Tran, S. Mayfield,
Z.L. Nikolov
BIOT – 272
Synthetic biology based multiple approaches for coenzyme
B12 production in Escherichia coli.
M. Noh, H. Lim, S. Park, G. Jung
BIOT - 273
Overcoming cellular barriers for enhancing polymer-mediated
transgene expression.
M. Christensen, J. Elmer, S. Barua, S. Eaton, L. GonzalezMalerva, J. Lehrman, J. LaBaer, K. Haynes, K. Rege
51
BIOT - 279
Engineering large pore mesoporous silica nanoparticles for
progene delivery application.
H.W. Omar, J.G. Croissant, L. Deng, N.M. Khashab
BIOT – 280
Design and formulation of novel nanoemulsions that inhibit
Mycobacterium smegmatis biofilm formation.
A. Kratzer, M. Brantley, C. Mageeney, W. Magilton, V. Ware,
B. Berger
BIOT - 281
Novel preparation and characterization of Epicatechin-3allopyranoside.
E.J. Parish, J. Wu, H. Ho, H. Honda, T. Wei
BIOT – 282
Novel development of a computational drug target prediction
method based on chemical similarity networks.
Y. Lo, E.J. Parish, H. Honda, T. Wei
BIOT – 283
Determination of optimum bead size for immobilized cell
fermentations for enhanced production of lovastatin by
Aspergillus terreus. G. Chun
BIOT – 284
Development of of fed-batch fermentation process for enhanced
production of cis-cis muconic acid using high-yielding mutant
cells of Corynebacterium glutamicum.
S. Kim, G. Chun, S. Byun, S. Seo, B. Lee, S. Park, Y. Kim, W.
Shin, S. Lee, E. Kim, D. Lee, S. Kim
BIOT - 290
Succinic acid production on xylose-enriched biorefinery
streams by Actinobacillus succinogenes in batch fermentation.
A. Mohagheghi, D. Salvachua, H. Smith, M. Bradfield, W.
Nicole, B. Black, M. Biddy, N. Dowe, G. Beckham
BIOT – 291
Advancing modeling and simulation in the biopharmaceutical
industry.
S.M. Hunt, J. Robinson, R.J. Todd
BIOT – 292
Developing a better scale-down model of a manufacturing
scale CHO bioreactor process using targeted transcriptomics.
A.G. Lee, W.D. Croughan, A. Lewis, N.R. Abu-Absi, M.C.
Borys, Z. Li
BIOT – 293
Influence of cell age on cell biological variability during early
process development.
P. Bolisetty, G. Tremml, S. Herzer, L. Paul
BIOT – 294
Process analytical technology (PAT) for bioprocessing.
A. Williams, B. Chavez, C. Agarabi, K.A. Brorson
BIOT – 295
Development of a heroin vaccine: Accurate assessment
of heroin binding to polyclonal antibodies using inhibition
equilibrium dialysis (IED) combined with ultra-high performance
liquid chromatography/tandem mass spectrometry (UPLC/MS/
MS).
O.B. Torres, R. Jalah, F. Li, J. Antoline, A. Jacobson, C. Alving,
K. Rice, G. Matyas
BIOT – 296
Bioglue for wet condition; dopa incorporated engineered
mussel adhesive proteins.
B. Yang, N. Ayyadurai, H. Yun, Y. Choi, J. Huang, Q. Lu, H.
Zeng, B. Hwang, H.J. Cha
BIOT – 297
Directed evolution methods for improving the affinity and
stability of Alzheimer’s antibody fragments.
K.E. Tiller, M. Julian, C. Lee, L. Rabia, J. Young, P.M. Tessier
BIOT – 298
Novel application to bioinformatics approaches of glycoprotein
domain identification and functional prediction.
E.J. Parish, Y. Lo, H. Honda, T. Wei
BIOT - 299
New approaches to RT-PCR analysis of animal tissue mRNA
expression for ChREBP gene sequence. E.J. Parish, H. Shyu,
H. Honda, T. Wei
BIOT – 300
Withdrawn
BIOT – 301
Evaluation of product antibody (mAb) heterogeneity in nonclonal cell pools for early pre-clinical development.
Y. Zhang, Z. Fang, M. Wang, L. Paul, W. Ding, J. Li, M. Gokhale,
J. Valente
BIOT – 302
Inference of cohort-specific HIV-1 kinetics.
J. White
Tuesday Evening
BIOT – 263
Process analytical technology solutions for real-time monitoring
and control of CHO bioprocesses.
E.R. Gibson
BIOT – 303
Interactions of LAH4-Lx and LAH4-Ax family of histidine-rich
amphipathic peptides with biomembrane models.
B.N. Suarez-Gonzalez, L. Vermeer, E. Glattard, A. Galy, D.
Fenard, B. Bechinger
BIOT – 304
Withdrawn
BIOT – 305
Decrease in transthyretin tetramer stability enhances inhibition
of beta-amyloid aggregation.
P. Mangrolia, R.M. Murphy
BIOT – 306
Well characterized and consistent quality carbohydrates
improves robustness and stability of protein formulation.
B. Thiyagarajan, M. Cox, C. Deily, N. Deorkar
52
BIOT – 308
Sequence and domain architecture of minicollagen from sea
anemone and their impacts on mechanical properties.
D. Jung, Y. Yang, J. Seo, Y. Choi, B. Hwang, H.J. Cha
BIOT – 309
Molecular design of self-assembled β-sheet-forming peptides
as amyloid inhibitors.
B. Ren, R. Hu, H. Chen, M. Zhang, F. Yang, J. Zheng
BIOT – 310
Probing a potential molecular link between β-Amyloid and
Human Islet Amyloid polypeptide via specific cross-sequence
amyloid interactions.
R. Hu, M. Zhang, H. Chen, B. Ren, F. Yang, J. Ma, B. Jiang, J.
Zheng
BIOT – 311
NMR analysis of protein-peptide interactions for in silico affinity
maturation of chromatographic peptide ligands.
C. Goodwine, D. Chandra, S. Timmick, N. Vecchiarello, S.M.
Cramer, P. Karande
BIOT – 312
Methods for discovering serum antibodies associated with
diseases via bacterial display peptide libraries.
J. Bozekowski, R. Pantazes, P.S. Daugherty
Tuesday Evening
BIOT – 313
Functional carbohydrate chip-based screening for substrate
specificity of sialyltransferase.
H. Heo, C. Kim, J. Seo, H.J. Cha
BIOT – 314
Compressive properties of cartilage.
F. Horkay, I. Horkayne Szakaly, E.K. Dimitiadis, P.J. Basser
BIOT – 315
Improving resolution and migration in non-reduced CE-SDS: A
tale of two desirable but antagonistic optimizations.
A. Shirke, R. Vanam, M. Marlow
BIOT – 316
Controlling mRNA translation in both prokaryotic and eukaryotic
cells using PUF domains.
J. Cao, M. Arha, C. Sudrik, X. Wu, A. Mukherjee, R. Kane
BIOT - 317
Folate binding protein: Therapeutic natural nanotechnology.
R.L. Merzel, S. Boutom, J. Chen, E. Marsh, M.M. Banaszak
Holl
53
BIOT – 318
Identifying solid binding polypeptides (SBP) with a high affinity
for cellulosic paper: Toward bio-tethering of functional devices.
T. Omokehinde, M.A. Allen
BIOT – 319
Probing the role of N and C-terminal regions in allosteric
regulation of Thermodesulfovibrio yellowstonii ADP-glucose
pyrophosphorylase.
C.R. Meyer, E. Yik, S. Kaur, E. Pushkarev, N. Duran, C. Diep,
L. Mulato, A. Avila
BIOT – 320
Engineered zinc finger proteins: A diagnostic tool for the
detection of methylated DNA.
A. Kini
BIOT – 321
Development of an analytical assay to characterize the
glycosylation pattern in monoclonal antibodies.
J. DeChiara, D. Radhakrishnan, A.S. Robinson, B. Ogunnaike
BIOT – 322
Characterisation
of
proteases
in
anion
exchange
chromatography for the bioprocess of a therapeutic enzyme.
D. Migani, M. Smales, D.G. Bracewell
BIOT – 323
EBA 2.0: Improving expanded bed adsorption technology for
bioproduct recovery.
V. Koppejan, E. van de Sandt, G. Ferreira, M. Ottens
BIOT – 324
Evaluation of eshmuno chromatography resins within a platform
approach for the purification of trastuzumab.
C.W. Richey, T. Roberts, T. Stone, S. Beard, K. Dhanasekharan
BIOT – 325
Nanofilter scalability: A comprehensive look into viral clearance
capabilities at all sizes.
A.H. Schwartz
BIOT – 326
Minimizing the impact of process buffer variations by
optimization of the Amsphere™ A3 Protein A resin design.
A. Naresh, B. Jeugt, G. Stroehlein, H. Shiho, M. Siwak
BIOT – 327
Ternary adsorption isotherms of proteins from high-throughput
experiments and multivariate analysis.
N. Field, S. Konstantinidis, A.K. Velayudhan
BIOT – 328
Evaluation of large scale disposable pre-packed columns for
use in biologics and vaccine purifications.
E. Wen, J. Konietzko, J. Jacob, F. Torres, J.G. Joyce
BIOT – 329
Leveraging solute surface analytics and high throughput
screening to define binding mechanisms of virus particles to
multimodal anion exchange resin.
M. Brown, K.A. Brorson, S. Lute, D.J. Roush, T.O. Linden
BIOT – 330
Increasing productivity of a mAb capture step: Alternate design
approaches.
L. Madhavan, C. Gerberich, J.R. Molek, A.C. Dumetz, G.J.
Terfloth
BIOT – 331
Withdrawn
BIOT - 332
Complications of accurately sizing filtration trains for the
purification of a sodium carboxymethylcellulose solution.
A. Steele
BIOT – 333
Targeted, high-throughput assessment of the selectivity of
chromatographic resins.
S. Timmick, S. Ruppel, S.M. Cramer
BIOT – 334
Characterization of peptide-functionalized agarose resin
supports for applications in affinity chromatography.
N. Vecchiarello, S. Timmick, D. Chandra, C.A. Goodwine, P.
Karande, S.M. Cramer
BIOT – 335
Optimal application of high area pleated devices for sterile
filtration.
S. Liu, S. Giglia, D. Durie, G. Kazan, R. Sylvia
BIOT – 336
Purification of therapeutic proteins by affinity precipitation using
peptide-conjugated smart biopolymers.
A. Mullerpatan, P. Karande, S.M. Cramer
BIOT – 337
Development of novel assays to predict viral hydrophobicity.
S. Johnson, M. Brown, S. Lute, K.A. Brorson
BIOT – 338
Chromatographic depth filters: Opportunities for turbidity
reduction and process intensification.
M. Mercaldi, S. Jain, M. Shifrin, W. Olsen
BIOT – 339
Lean green efficiency machine: How to continuously improve
your work environment.
R.C. Massicotte
BIOT – 340
Withdrawn
BIOT – 341
Evaluation of body-feed assisted cell culture clarification in
single-use device.
X. Zhao, M. Shearer
BIOT - 342
Impact of buffer exchange and protein concentration on
biologics quality during ultrafiltration and diafiltration (UF/DF).
E. Schutsky, A. Arunkumar, R.K. Swanson, D. Yu, A.T.
Lewandowski, N. Singh, Z. Li
BIOT – 343
Withdrawn
BIOT – 344
Automating mAb purification workflows: Combining tandem
and multi-D purification methodology with sample analysis.
J. Habel
BIOT – 345
Bispecific antibodies: Screening from traditional to mixed-mode
chromatography modalities for a purification solution.
T. Matos, H. Karkov, G. Andersen, L. Sejergaard, H. Ahmadian,
S.M. Cramer
BIOT – 346
Virus detection using restricted-access adsorbents.
U. Patil, S.P. Dhamane, M. Adhikari, A. Hagstrom, U. Strych, K.
Kourentzi, R.C. Willson
BIOT – 347
Modes of controlling charge variant distribution in mAb
production by upstream and downstream process parameters.
T. Bjorkman, L. Kärf, A. Ljunglöf, T. Falkman, A. Vitina
Tuesday Evening
BIOT – 307
Investigating mechanisms and levers to control trisulfide
formation in mAbs produced in CHO cell culture.
B. Wong, M. Shiratori, V. Grosskopf, A. Meier, J. Wuu, M.
Gawlitzek, S. Meier
BIOT – 348
Investigation of the biophysics of host cell protein associations
with monoclonal antibodies.
S. Ranjan, W. Chung, M. Zhu, D. Robbins, S.M. Cramer
BIOT – 349
Enhancement of dynamic body feed clarification performance
using chitosan treatment.
H. Kaligotla
BIOT - 350
Computational fluid dynamics (CFD) modeling of flow and
fouling in hollow fiber tangential flow (HFTFF) and alternating
tangential flow (HFATF) filtration of a perfusion bioreactor.
F. Radoniqi, S. Wang, H. Zhang, P. Shamlou, J.L. Coffman
54
BIOT – 352
Why synthesize protein–polymer conjugates? The stability and
activity of chymotrypsin-polymer bioconjugates synthesized by
RAFT. S. Sloane, R. Falatach, D. Konkolewicz, J. Berberich,
R.C. Page, S. Averick
BIOT –353 Impact of pre-filter chemistry and operating conditio
ns on virus filter performance.
M. Luther, J. Weaver, A. Kantardjieff, R. Alford
BIOT - 354
Electrical stimulation of human dermal fibroblasts and
quantification of collagen, elastin, and collagenase.
E. Nguyen, K. Columna, J. Wishner, K. Slowinska
BIOT – 355
Multiphoton spectroscopy on Thioflavin T: A nonlinear approach
for amyloid detection.
J. Donnelly, F.E. Hernandez
BIOT - 356
Withdrawn
BIOT – 357
Using rheology as an indicator of cell lysis in E. coli fermentation
to determine optimal harvest time and prevent product loss.
J.M. Newton, Y. Zhou, J. Vlahopoulou, D. Schofield
BIOT – 363
Design and evaluation of advanced Amsphere™ A3 protein A
chromatography resin: The relationships and considerations of
bead structure, pore structure, surface chemistries and ligand
design on affinity resin performance targets.
A. Naresh, M. Siwak, M. Hanamura, M. Higami, T. Matsuda, S.
Nakamura, Y. Okano
BIOT – 364
Expanded toolbox for various MAb purification challenges. A.
Gronberg, L. Kärf, K. Nilsson Välimaa, C. Brink, A. Edman
Orlefors
BIOT - 365
Biotreatment of slaughterhouse wastewater accompanied with
electricity generation in mictrobial fuel cell.
Z.Z. Ismail, A. Mohammed
BIOT – 366
Two-dimensional isobutyl acetate production pathways to
improve carbon yield.
Y. Tashiro, S. Desai, S. Atsumi
BIOT – 367
Automated nucleic acid extraction workstation based on
magnetic separation.
Y. Wu, N. He
Tuesday Evening
BIOT – 358
Rapid discovery of peptidomimetics as antibody alternatives
via epitope-targeted screening.
J. Liang, A. Nag, S. Das, D. Bunck, A. Umeda, A. McCarthy, A.
Mishra, J. Heath, J.R. Heath
BIOT – 368
Bioinformatical analysis of spider silk protein sequences:
Finding relation with evolution and mechanical properties.
D. Jung, Y. Yang, H.J. Cha
BIOT – 359
X-ray computed tomography for the representation of
chromatographic structure at the individual bead and packed
bed scales.
T. Johnson, P.R. Shearing, L. Peter, D.G. Bracewell
BIOT – 369
Cross-seeding amyloid interactions between human IAPP and
Rat IAPP.
M. Zhang, R. Hu, H. Chen, B. Ren, M. Yang, J. Ma, B. Jiang,
J. Zheng
BIOT – 360
Engineering fatty acid responsive elements for metabolic
engineering in oleaginous yeast, Yarrowia lipolytic.
M. Shabbir-Hussain, P. Baker, M.A. Blenner
BIOT – 370
Cyclic peptide ligands for the purification of erythropoietin by
affinity chromatography.
W.S. Kish, A. Naik, H. Sachi, B.G. Bobay, S. Menegatti, R.G.
Carbonell
BIOT – 361
Use of chromatography simulation and fundamental
measurements to determine equivalent IEX loading under
different bed compression conditions for a fusion protein.
X. Xu, C. Huang, S. Traylor, N. Zhang, Z. Li
55
BIOT – 362
Polyelectrolyte multilayers loaded with antifungal β-peptide
reduce the formation of C. albicans biofilms in film-coated
catheter segments in vitro and in vivo.
N. Raman, M. Lee, K. Marchillo, A. Rodríguez López, D. Andes,
S. Palecek, D.M. Lynn
BIOT – 371
Molecular recognition of novel heptapeptides at Fc-binding
sites from molecular dynamics simulations.
X. Sun, J. Weaver, R. Wickramasinghe, X. Qian
BIOT – 372
Overcoming buffer challenges in downstream processes by Inline conditioning.
K. Busson, E. Carredano, H. Martin
BIOT - 383
Rates of reaction of the sulphydryl groups of some derivatives of
human hemoglobin A with 5, 5-dithiobis-(2-nitrobenzoic acid).
F.u. Akhigbe
BIOT – 373
Developmental approaches for overloaded cation exchange
chromatography.
Y. Yigzaw, A. Mehta, C. Williams, K. Nagpal, J. Yang
BIOT – 384
Rapid generation of dCas9-regulated, orthogonally repressible
hybrid T7-lac promoter sets for modular, tunable control of
metabolic pathway fluxes in E. coli.
B. Cress, J.A. Jones, D. Kim, Q. Leitz, R.J. Linhardt, M. Koffas
BIOT – 374
Integration of platform high throughput technologies in
biopharmaceutical downstream process development.
G. Ma, Y. Feng, C. Gerberich, A. Dumetz, G.J. Terfloth, K.E.
Goklen
BIOT – 385
Design of two new protein A resins.
H.J. Johansson
BIOT – 375
Maximizing productivity of protein A capture.
I. Savoy, B. Kalbfuss-Zimmermann, S. Rueger, L.W. Pampel
BIOT – 386
Model-based process development for biopharmaceuticals.
S. Pirrung, A. Hanke, L.A. van der Wielen, P.D. Verhaert, E.
van de Sandt, M. Eppink, M. Ottens
BIOT – 376
Remediation of crude oil-polluted agriculturals oil by Fimbristylis
litoralis.
E.O. Nwaichi, G. Iwo, G. Attuah
BIOT – 387
Impact of channel shear effects on process hydraulics and
biologics quality during ultrafiltration and diafiltration (UF/DF).
A. Arunkumar, E. Schutsky, N. Singh
BIOT – 377
Purification and characterization of the Drosophila melanogaster
(Dm) IKKβ/IKKγ complex.
W. Rogers, T. McDowell, T. Huxford
BIOT - 388
Platelet-targeting thiol reduction sensor detects protein disulfide
isomerase activity on activated platelets in mouse and human
blood under flow.
S. Zhu, J. Welsh, L. Brass, S. Diamond
BIOT – 378
Withdrawn
BIOT - 379
Base-free aerobic oxidation of 5-hydroxymethylfurfural to
2,5-furandicarboxylic acid over Pt/C-O-Mg catalyst.
X. Han, Y. Wang
BIOT – 380
Multiple reaction pathways and mechanisms of cellulose
conversion into bio-platform molecules in water and alcohol
medium.
D. Ding, Y. Wang
BIOT – 381
Bioactive crosslinked pepsin nanoaggregates above pH 5.0.
T. Akkas, A. Zakharyuta, A. Taralp
BIOT – 382
Bottom-up approach to describe protein-protein interactions in
a continuum mechanics biomolecular model of fibrin.
A. Solernou, D. Read, S. Harris, O. Harlen, K.A. Smith, S.P.
Muench
BIOT - 389
Pitting rational against random: Improving light-driven P450
biocatalysts.
M. Kato, L. Baragan, L. Tang, L.E. Cheruzel
Tuesday Evening
BIOT – 351
Chromatographic quantification of a complex protein mixture
using problem-specific deconvolution.
K. Jurlewicz, S. Konstantinidis, A.K. Velayudhan
BIOT – 390
Simple process strategies to increase the utilization of protein A
media in clinical manufacturing.
A. Becerra-Arteaga
BIOT – 391
Withdrawn
BIOT – 392
Engineering affinity, selectivity and elutability into peptides for
their development as ligands for downstream bioprocessing of
non-mAb biologics.
D. Chandra, S. Timmick, N. Vecchiarello, C. Goodwine, S.M.
Cramer, P. Karande
BIOT – 393
Withdrawn
56
BIOT – 395
Enhanced bioadsorption of rare earth elements through cell
surface display of lanthanide binding tag.
D.M. Park, M. Yung, A. Eslamimanesh, D.W. Reed, Y. Fujita,
A.M. Anderko, Y. Jiao
BIOT – 396
QSAR models for prediction of chromatographic behavior of
homologous Fab variants.
J. Robinson, H. Karkov, J. Woo, B.O. Krogh, H. Ahmadian,
S.M. Cramer
BIOT – 397
Dynamic modeling of human coagulation cascade using
reduced order effective kinetic models.
A. Sagar
BIOT – 398
Polymer grafting to functionalize chromatography media for
purification of monoclonal antibodies.
J.U. Hansson, E. Brekkan, J. Bengtsson
BIOT – 399
Recombinant DNA and its medical benefits: Synthesis of
insulin.
B.B. Ibrahim
BIOT – 400
New enabling technologies for continuous final formulation of
mAbs.
E. Ayturk
Tuesday Evening
BIOT – 401
Analyzing the mechanical properties of three synthetic based
biopolymers.
R. Anderson
BIOT – 402
Evaluation of flow-through hydrophobic interaction and
hydroxyapatite chromatography steps for the removal of
aggregates in the purification of monoclonal antibody TBLmAb-01.
W. Evans, A. Chakrabarti
BIOT – 403
Capturing carbon dioxide in hydroxyacid-derived bio-plastics.
I.s. Al Rowaihi, S. Groetzinger, K. Dietrich, J. Eppinger
57
BIOT – 404
Single-molecule kinetics of protein adsorption on thin nylon 6,6
films.
H. Shen, L.J. Tauzin, W. Wang, B. Hoener, L. Kisley, A.
Hoggard, C.F. Landes
BIOT – 405
More affordable version of therapeutic protein recombinant
Streptokinase to address over a million ST elevated Micardial
Infarction (STEMI) deaths in part of the world where affordibility
is the key.
D. Ghosh, S. Paul, R.K. Gupta
BIOT – 406
Improving the alkali stability of the kappa light chain-binding
polypeptide from domain of peptostreptococcus protein L.
R. Palmgren, G. Rodrigo, A. Mattsson, T. Bjorkman, J. Vasic,
E. Monie, M. Ander, G. Bauren
BIOT – 415
Engineering the ligand affinity and specificity of the human
adenosine A 2A receptor.
J. Yoo, P.S. Daugherty, M.A. O’Malley
BIOT – 416
Computational investigation of mutational impact on terpene
synthases.
L. Li, C. Pirie
BIOT – 417
Use of Fc gamma receptors as chromatographic ligands to
enrich high affinity subclasses and glycoforms from human
plasma IgG. A. Boesch, T. Chu, H. Kappel, A. Mahan, G. Alter,
M. Ackerman
BIOT – 407
Keynote: Emerging technologies.
E. Shusta
BIOT – 408
Optimum pulse mode selection for biodiesel production using
ultrasound irradiations.
E. Martinez-Guerra, V. Gude
BIOT – 409
Mapping the collective response of water to predict biomolecular
interaction interfaces.
R.C. Remsing, J.D. Weeks
BIOT – 410
Molecular dynamics simulations of proteins coupled to
nanoparticles.
S. Sen, P. Kral
Tuesday Evening
BIOT - 394
Tailoring continuous protein A mAb capture for facility fit and
process economics.
X. Gjoka, K. Rogler, M. Bisschops, R. Gantier, M. Schofield
BIOT - 411
Withdrawn
BIOT - 412
Moving towards a sterile chromatography platform for virus
purification.
J. Konietzko, S. Wang, A. Kristopeit, M. Wenger, J. Joyce
BIOT – 413
Computational reconstruction of the protein secretion pathway
in CHO cells.
J.M. Gutierrez Bugarin, A. Feizi, N.E. Lewis
BIOT - 414
Examination of multivariate model generation using UV-vis
spectral data for mAb titer.
J. Miller, J.L. Coffman, P. Shamlou
58
C
D
E
F
Upstream Processes
Papers 418 - 425
8:30-11:30 AM
Crystal II
Presiding: N. Jacob, G. Sriram
Leveraging Fundamentals for Accelerated
Downstream Process Development
Papers 426 - 433
8:30-11:30 AM
Emerald Ballroom
Presiding: K. Lacki, V. Natarajan
Biomol. & Biophys Processes
Protein Conjugates and Materials I
Papers 434 - 440
8:30-11:30 AM
Diamond I
Presiding: J. Berberich, J. A. Van Deventer
New Tools and Approaches II
Area Coordinators: J. Latone, G. Thurber, I. Wheeldon
Papers 441 - 448
8:30-11:30 AM
Opal
Presiding: N. Argarwal, A. Chatterjee
QbD Case Studies
Process Characterization
Papers 449 - 455
8:30-11:30 AM
Crystal I
Area Coordinators: C. Torigoe, S. Singh
Presiding: S. Singh, M. Westoby
Processes
Protein Structure and Function
Papers 456 - 463
8:30-11:30 AM
Diamond II
Upstream Processes
Presiding: N. Jacob, G. Sriram
BIOT 418 - 8:30
Large scale dynamic models of metabolism as a
tool for analysis and design of metabolic engineering and
synthetic biology strategies.
G. Fengos, L. Miskovic, V. Hatzimanikatis
BIOT 419 - 8:50
Characterizing strain variation in engineered E. coli using a
multi-omics based workflow.
E.C. Brunk, K. George, J.D. Keasling, B. Palsson, T. Lee
BIOT 420 - 9:10
Hierarchy of decisions that impact the accuracy of tissuespecific metabolic models.
S. Opdam, N.E. Lewis
BIOT 421 - 9:30
Technical evaluation of RNA-Seq and microarray approaches
in comparative transcriptomics analysis of CHO cells.
C. Chen, H. Le, C. Goudar
9:50 Intermission
Leveraging Fundamentals for Accelerated
Downstream Process Development
Presiding: K. Lacki, V. Natarajan
BIOT 426 - 8:30
Ultrafiltration behavior of highly concentrated monoclonal
antibodies.
E. Binabaji, A.L. Zydney, J. Ma
BIOT 427 - 8:50
Thermodynamically based modelling of effects of ethanol and
potassium chloride on reversed-phase chromatography.
K. Johansson, S.S. Frederiksen, M.P. Breil, E. Broberg
Hansen, B. Nilsson
BIOT 428 - 9:10
Mechanistic modeling of antibody transport, adsorption
equilibrium, and uptake kinetics in protein A chromatography
resins with PEGylated ligand.
J. Weinberg, T.M. Przybycien
BIOT 429 - 9:30
Mechanistic basis for adsorptive selectivity in conventional and
dextran-functionalized ion exchangers: Model and experiment.
A.M. Lenhoff, C. Narambuena, I. Szleifer, J.M. Angelo
9:50 Intermission
BIOT 422 - 10:10
Directed evolution of Escherichia coli quorum sensing promoter
region of the lsrACDBFG operon: A tool for synthetic biology
systems and protein expression.
P. Hauk, R. Mckay, C. Virgile, H. Ueda, M. Ostermeier, W.E.
Bentley
BIOT 430 - 10:10
Enhancing the power of HTPD with empirical interpolation
models to predict elution behavior at high protein loads.
A. Creasy, J. Calzada, G. Barker, S. Herzer, L. Paul, S. Rieble,
G. Carta
BIOT 423 - 10:30
Evaluation of public genome references for RNA-Seq data
analysis in Chinese hamster ovary cells.
H. Le, C. Chen, C. Goudar
BIOT 431 - 10:30
Predicting cation exchange Fab purification using micro-tip
chromatography and general rate modelling.
S. Gerontas, S. Kong, N.J. Titchener-Hooker
BIOT 424 - 10:50
Applying adaptive laboratory evolution to engineer platform
strains.
A. Feist
BIOT 432 - 10:50
Scalability of mechanistic models for ion exchange
chromatography.
T. Huuk, T. Hahn, J. Griesbach, S. Hepbildikler, J. Hubbuch
BIOT 425 - 11:10
Genome-scale stoichiometric model of poplar for investigation
of woody plant metabolism.
N. Boruah, A. Misra, M. Simons, G. Coleman, G. Sriram
BIOT 433 - 11:10
Withdrawn
Presiding: W. Pomerantz, R. St. John
59
Wednesday Morning
A
B
Wednesday Morning
60
Protein Conjugates & Materials I
Presiding: J. Berberich, J. Van Deventer
BIOT 434 - 8:30
Responsive polymer-protein conjugates: From stimulusinduced self-assembly to the treatment of accelerated bone
disorders.
B.S. Sumerlin, B. Tucker, J.D. Stewart, J. Aguirre
BIOT 435 - 9:10
Withdrawn
BIOT 436 - 9:30
SuFEx bioconjugation. S. Li, E. Kallick, S. Averick
9:50 Intermission
BIOT 437 - 10:10
Densely modified chymotrypsin-polymer conjugates with
increased stability to low pH.
C. Cummings, A.J. Russell
BIOT 438 - 10:30
Engineered knottin peptide-drug conjugates selectively deliver
small molecules to brain tumors in mice.
N. Cox, J.R. Kintzing, N.V. Currier, S.E. Ackerman, S.M.
DePorter, M. Smith, G.A. Grant, J. Cochran
BIOT 439 - 10:50
Self-assembly of temperature-responsive protein-polymer
bioconjugates.
J. Li, D. Moatsou, A. Ranji, A. Pitto-Barry, I. Ntai, R.K. O’Reilly,
M. Jewett
Wednesday Morning
61
Biot 440 - 11:10
Novel biocompatible modifications of phage-displayed peptide
libraries to generate genetically-encoded libraries of peptide
derivatives.
R. Derda
New Tools & Approaches II
8:30 - 11:30AM, Opal
Presiding: N. Argarwal, A. Chatterjee
QbD Case Studies: Process Characterization
Presiding: S. Singh, M. Westoby
Protein Structure & Function
Presiding: W. Pomerantz, R. St. John
BIOT 441 - 8:30
Development of a SERS-based biomolecular assay.
T. Chuong, A. Pallaoro, G.D. Stucky, M. Moskovits, T. Soh
BIOT 449 - 8:30
Bioreactor scale-down model verification case study.
M. Mollet, R. Patel, G. Miro-Quesada, L. Qu
BIOT 456 - 8:30
Exploring the space of sense codon reassignment in E. coli.
J. Fisk
BIOT 422 - 8:50
Lysate of engineered Escherichia coli supports high-level
conversion of glucose to 2,3-butanediol.
J. Kay, M. Jewett
BIOT 450 - 8:50
High-throughput chromatography as qualified scale-down
model: Challenges and opportunities.
J. Aucamp, J. Hitzler, S. Urig, L.W. Pampel, J. Shultz
BIOT 457 - 8:50
Engineering leave-one-out-GFP based biosensors.
K. Fraser, S. Banerjee, C. Schenkelberg, K. Zhang, D. Chan,
C. Thornton, C. Lamberson, V. Jones, A. Choi, R. Altshuler,
J.S. Dordick, C. Bystroff
BIOT 443 - 9:10
Exploring alternative materials to fabricate microfluidic gradient
generators to study algal growth and migration.
A.T. Melvin
QbD six years after A-mAb: What we expected, what happened,
and what now.
J. Erickson
BIOT 444 - 9:30
Sequence-specific nucleic acid detection based on ionic
current measurement through a glass nanopore.
B. Koo, A. Yorita, H. Monbouquette
9:50 Intermission
9:50 Intermission
BIOT 452 - 10:05
First principles modeling towards accelerated process
development: Experiences and prospects of computational
model-based filing. C. Undey, T. Larsen, M. Coufal, O.
Kaltenbrunner, B. Kuhn, T. Mire-Sluis
BIOT 458 - 9:10
Solvent-free functional biofluids as a route for retained structure
and improved protein stability in nonaqueous environments.
A. Brogan, J.P. Hallett
BIOT 459 - 9:30
Observing structural changes in biopharmaceutical proteins as
a function of concentration.
C.W. Meuse
9:50 Intermission
BIOT 445 - 10:10
Construction of robust, tunable genetic circuits to prevent
infectious diseases.
T. Shopera, W.R. Henson, A. Ng, Y. Lee, K. Ng, C. Johnson, A.
Hoynes-O’Connor, T. Moon
BIOT 453 - 10:25
Ultrafiltration diafiltration: Process characterization and process
validation.
Z. Begum
BIOT 460 - 10:10
Nanobody activation immunotherapeutics that selectively
destroy cancer cells.
A. Ta, M. Gray, R. Tao, B. McNaughton
BIOT 446 - 10:30
Controlling cells through RNA Folding: Towards design
principles for RNA engineering.
J.B. Lucks
BIOT 454 - 10:45
Commercial antibody formulation development using quality by
design elements.
M. Bhattacharya, S. Mehta, M. Dey
BIOT 461 - 10:30
Dissecting the initial steps of heme uptake between an outer
membrane receptor protein of Shigella dysenteriae and
hemoglobin by phage display.
L. Meneghini, G.A. Weiss
BIOT 447 - 10:50
Advances in the development of a protein catalyzed capture
(PCC) agent for the L1R protein of vaccinia as an antibody
alternative for biosurveillance in austere environments.
M. Coppock, D. Cangelosi, S. Das, J.R. Heath, D. StratisCullum
BIOT 455 - 11:05
Use of quality by design approach to optimize downstream
cross-step operation space of an Fc fusion protein.
C. Huang, X. Xu, Z. Li
BIOT 448 - 11:10
Microfluidic gradient generator-flow cell system as a novel tool
to study biofilm development and control.
Y. Zhang, Y. Cohen, B. Cao
Quality by Design
BIOT 462 - 10:50
N- and C-terminal domains differentially contribute to the
structure and function of dystrophin and utrophin tandem
calponin-homology domains.
S. Singh, S. Bandi, K. Mallela
BIOT 463- 11:10
Characterization of knob-in-hole assembly variants in support
of bispecific antibody development.
V. Lundin
Wednesday Morning
62
C
Upstream Processes
Papers 464 - 471
2:00 - 5:00 PM
Crystal II
Presiding: M. Brynildsen, A. Yongky
Technology Transfer, Scale-up and Scale-Down
Papers 472 - 478
2:00 - 5:00 PM
Emerald Ballroom
Papers 479 - 486
2:00 - 5:00 PM
Diamond I
Protein Conjugates and Materials II
Presiding: J. Van Deventer, J. Berberich
D
E
**
63
Protein and Molecular Engineering
Papers 487 - 494
2:00 - 5:00 PM
Opal
Presiding: R. Bott, M. Kim
QbD Case Studies
Process Validation
Papers 495 - 502
2:00 - 5:00 PM
Crystal I
Area Coordinators: C. Torigoe, S. Singh
Presiding: K. Barnthouse, M. Trexler-Schmidt
Biotechnology & Bioengineering Daniel I. C.
Wang Award Lecture
(Sponsored by: Wiley & Sons)
Technology Transfer, Scale-up & Scale-Down
Presiding: P. Alfonso, J. Cyganowski
BIOT 464 - 2:00
New yeast strain to produce fuels and chemicals instead of
ethanol.
J. Avalos, L. Grundy
BIOT 465 - 2:20
Carbon-efficient conversion of methanol to chemical
commodities via synthetic methanol condensation cycle (MCC).
C. Chen, I.W. Bogorad, T. Wu, M. Theisen, J.C. Liao
yeast
BIOT 467 - 3:00
Withdrawn
3:20 Intermission
BIOT 468 - 3:40
Engineering thiolase enzyme specificity.
Y. Tarasova, B. Bonk, B. Tidor, K.L. Jones Prather
Presiding: M. Brynildsen, A. Yongky
BIOT 466 - 2:40
Identifying ester biosynthesis pathways in the
Kluyveromyces marxianus. A. Loebs, I.R. Wheeldon
Presiding: P. Alfonso, J. Cyganowski
Processes
Upstream Processes
BIOT 469 - 4:00
Accelerating biocatalysis in thin films.
J. Britton, C.L. Raston, G.A. Weiss
BIOT 470 - 4:20
Integrating metabolic engineering and electrocatalysis for the
production of polyamides from sugar.
M. Suastegui, J. Matthiesen, J. Tessonnier, Z. Shao
BIOT 471 - 4:40
Utilizing electrochemical bioreactors for efficient biofuel and
chemical applications.
C.S. Morrison, R.S. Kane, J.S. Dordick, D.R. Dodds, W.B.
Armiger, M. Koffas
Downstream processes: Past, present and future.
J. Myers
Wednesday Afternoon
A
B
Wednesday Afternoon
BIOT 473 - 2:40
Multiproduct resin reuse for biopharmaceuticals: Application to
clinical and commercial manufacturing.
R. Sharnez, K. Mehta, R.G. Soderquist
BIOT 474 - 3:00
Learning from small-scale process characterization studies
gone wrong: A case study in chromatography resin lifetime.
P.R. Smith, A. Pike, B.F. Marques, K.E. Goklen
3:20 Intermission
BIOT 475 - 3:40
Implementation of a single-use mixer as a point-of-use and
disposable heat exchanger in cGMP downstream processing.
B. Youchak, W. Bell, R. O’Keeffe, M. Elsner
BIOT 476 - 4:00
Investigation of low molecular weight species and
implementation of mitigation strategies into large scale biologics
manufacturing.
Z. Tan, A.T. Lewandowski, V. Ehamparanathan, B. Ng, E.
Schutsky, M. Mayani, D. Yu, R. Martel, S. Egan, M. Krajcovic, K.
Aron, M. Peck, J. Ray, N. Singh, N. Zhang, C. Du, A. Borwankar,
M. Lu, N. Hershey, Y. Huang, L. Tao, M.C. Borys, Z. Li
BIOT 477 - 4:20
Leveraging Monte Carlo simulations and mechanistic models
to optimize a high concentration UFDF process within facility
constraints.
A. Brinkmann, M. Westoby
BIOT 478 - 4:40
Optimization of bio-molecule separation by combining
microscale filtration and design-of-experiment methods.
A.S. Kazemi, K. Kawka, D.R. Latulippe
5:00 - 6:00 PM
Emerald Ballroom
64
Protein Conjugates & Materials II
Presiding: J. Berberich, J. Van Deventer
BIOT 479 - 2:00
Protein nanoparticle conjugates for cancer immunotherapy.
M. Neek, N. Molino, J. Tucker, E.L. Nelson, S. Wang
BIOT 480 - 2:20
Tuning the activity and stability of protein-polymer conjugates
by attaching functional polymers.
M. Lucius, D. Konkolewicz, J. Berberich, R.C. Page
BIOT 481 - 2:40
Whole animal to single cell near infrared fluorescent imaging of
antibody drug conjugate metabolism.
C. Cilliers, G.M. Thurber
BIOT 482 - 3:00
In vivo synthesis of nanoparticles through evolved peptide
lipidation.
R. Menacho Melgar, M. Lynch
3:20 Intermission
BIOT 483 - 3:40
Platform for constructing, evaluating, and
bioconjugates on the yeast surface.
J.A. Van Deventer, D. Le, J. Zhao, R.L. Kelly
Protein & Molecular Engineering
2:00 - 5:00PM, Opal
Presiding: R. Bott, M. Kim
BIOT 487 - 2:00
Artificial TCA cycle metabolon: Direct evidence for metabolon
formation and substrate channeling.
K. Garcia, B. Bulutoglu, F. Wu, S.D. Minteer, S. Banta
BIOT 488 - 2:20
Osteogenic differentiation of stem cells promoted by the
assemblies of genetically engineered bacteriophage.
C. Mao
BIOT 489 - 2:40
Bacterial inner membrane display for screening a naïve
antibody library to isolate antibodies that bind survivin.
P. Moghaddam-Taaheri, A.J. Karlsson
BIOT 490 - 3:00
Rational design and development of polysialic acid-binding
peptides: Implications in neural stem cell purification.
D. Shastry, P. Karande
3:20 Intermission
screening
BIOT 484 - 4:00
Point of care device for detection of myeloperoxidase as a
wound infection marker.
E. Ramon, A. Francesko, T. Tzanov
BIOT 485 - 4:20
Biofabrication of protein networks via tobacco mosaic virusvirus like particles (TMV-VLP): 3D scaffold interfaces.
N. Bhokisham, K. Wang, A. Brown, G.F. Payne, J.N. Culver,
W.E. Bentley
BIOT 486 - 4:40
Wiring of redox enzymes using a collagen heterotrimer protein.
H.F. Ozbakir, J. Brisendine, R.L. Koder, S. Banta
65
BIOT 491 - 3:40
Constructing new bioluminescent tools with minimally perturbed
luciferins.
C.M. Rathbun, R.C. Steinhardt, W.B. Porterfield, K. Jones, D.
McCutcheon, M.A. Paley, J.A. Prescher
BIOT 492 - 4:00
Circle Akt In: Macrocycles against phosphorylated Akt.
A. Nag, S. Das, J.R. Heath
BIOT 493 - 4:20
Switchable protein sensors and enzyme reactions based on
dynamic DNA assembly.
R.P. Chen, D. Blackstock, Q. Sun, W. Chen
BIOT 494 - 4:40
RNA length affects form and function of the Type I CRISPRCas surveillance complex.
M. Luo, C. Beisel
Wednesday Afternoon
Wednesday Afternoon
Quality by Design
QbD Case Studies: Process Validation
Presiding: K. Barnthouse, M. Schmidt
BIOT 495 - 2:00
Manufacturability assessment tool for effective decisionmaking and efficient process development: Development and
applications. K. Brower, R.A. Patil, D.A. Shah, J. Walther, N.M.
Troccoli, T.A. Vetter, V. Warikoo
BIOT 496 - 2:20
Process linkage approach for establishing critical quality
attribute target ranges.
S. Khoo, M. Mun, M.T. Schmidt
BIOT 497 - 2:40
Use of relevant process history for the determination of critical
process parameters.
B. Huffman, J. Cundy, K. Sunasara
BIOT 498 - 3:00
Using characterization data to support process validation: A
Monte Carlo approach.
C. Thompson, T. Linke, A. Hunter, G. Miro-Quesada, X. Wang
3:20 Intermission
BIOT 499 - 3:40
Defining process design space for a therapeutic monoclonal
antibody: Clearance of phospholipase B-Like 2 (PLBL2), a
CHO host-cell protein impurity, by hydrophobic interaction
chromatography.
A. Sanchez, J. Bill, S. Fisher
BIOT 500 - 4:00
Multivariate process monitoring towards enhanced continued
process verification.
C. Undey, S. Oruklu, B. Looze, C. Garvin
BIOT 501 - 4:20
Designing resin end of lifetime studies and their application in
the three stages of process validation.
A. Magill, E.S. Wilhelm, J. Weiss, D. Saini
BIOT 502 - 4:40
Improving the control strategy of legacy products using quality
by design principles.
D. Vu, A. Hagstrom, G. Miro-Quesada, G. Ferreira
66
Engineering Natural Products Biosynthesis
Presiding: S. Ahuja, W. Zhang
Non-Chromatographic Separations and Process
Integration I
Papers 511 - 518
8:30-11:30 AM
Emerald Ballroom
Presiding: H. Ahmadian, A. Lenhoff
C
General Topics in Biomolecular and Biophysical
Processes
Papers 519 - 525
8:30-11:30 AM
Diamond I
D
E
F
**
67
Upstream Processes
Papers 503 - 510
8:30-11:30 AM
Crystal II
Presiding: A. Lajimi, H. Sikes
Disease and Biomedical Applications I
Papers 526 - 533
8:30-11:30 AM
Opal
Presiding: C. Chen, S. Servoss
QbD Case Studies
Papers 534 - 537
8:30-11:30 AM
Crystal I
Area Coordinators: S. Singh, C. Torigoe
Presiding: S. Abraham, C. Agarbi
Upstream Processes
Control of Protein Quality Attributes
Papers 538 - 545
8:30-11:30 AM
Diamond II
Presiding: V. Janakiraman, T. Whitehead
Biotechnology & Bioengineering Awards
Presentation and Gaden Award Lecture
(Sponsored by: Wiley & Sons)
Upstream Processes
Engineering Natural Products Biosynthesis
Presiding: S. Ahuja, W. Zhang
Non-Chromatographic Separations & Process
Integration I
Presiding: H. Ahmadian, A. Lenhoff
BIOT 503 - 8:30
Biosynthetic engineering of new anti-infectives and anticancer
agents.
T. Mahmud
BIOT 511 - 8:30
Investigations of alternative clarification methods to obviate
protein A on-column precipitation.
K. Galipeau, J. Caron, C. Gillespie
BIOT 504 - 8:50
Engineered biosynthesis of terminal alkyne-tagged natural
products.
W. Zhang
BIOT 512 - 8:50
Development of an affinity precipitation process using protein
nanocages as a non-chromatographic alternative to platform
therapeutic antibody purification.
A. Swartz, Q. Sun, W. Chen
BIOT 505 - 9:10
PqqD homolog is a standalone leader peptide binding protein in
lasso peptide biosynthesis.
W.L. Cheung, M. Chen, M. Maksimov, A. Link
BIOT 506 - 9:30
New technologies to understand and engineer carrier protein
mediated pathways.
M.D. Burkart
9:50 Intermission
BIOT 513 - 9:10
Novel approach for non-chromatographic purification of
monoclonal antibodies from cell culture supernatant.
R.B. Wollacott, R. Sharpe, D. Seeman, S.S. Ozturk, P.L. Dubin
BIOT 514 - 9:30
Development of robust impurity clearance methods using
chromatographic and non-chromatographic techniques.
S. Chollangi, N. Singh, M. Peck, K. Sing, Y. Li
9:50 Intermission
BIOT 507 - 10:10
Directing biosynthesis with diversity-generating metabolism.
E.W. Schmidt
BIOT 508 - 10:30
Heterologous microbial biosynthesis of natural
analogues using dynamic metabolic control.
Z. Ye, M. Lynch
Thursday Morning
A
B
Thursday Morning
product
BIOT 509 - 10:50
Comprehensive re-engineering and synthesis of a complex
biosynthetic gene cluster.
J. Kim, K. Louie, T. Northen, S. Deutsch, C. Hoover
BIOT 510 - 11:10
Strategies toward discovering new natural products from fungal
species.
Y. Tang
BIOT 515 - 10:10
Benzonase® endonuclease for improved primary recovery in an
E. coli-based process for Fab production.
A. Stein, A. Heinen-Kreuzig, A. Kiesewetter, N. Lutz
BIOT 516 - 10:30
Purification process for heparin using the pH responsive
behavior of chitosan.
U. Bhaskar, L. Fu, J.S. Dordick, R.J. Linhardt
BIOT 517 - 10:50
Assessment of beta glucan leachables originating from
cellulose based depth filtration media.
A. Gupta, D. Kinzlmaier, K.M. Pizzelli, E.M. Goodrich
BIOT 518 - 11:10
Progress in development of size exclusion filter paper material
for virus removal applications.
A. Mihranyan
11:30 AM-12:30 PM
Emerald Ballroom
68
General Topics in Biomol. & Biophys. Processes
Presiding: A. Laijimi, H. Sikes
Thursday Morning
BIOT 519 - 8:30
Withdrawn
BIOT 520 - 9:10
Effect of histatin-5 mutations on proteolytic degradation by
Candida albicans secreted aspartic proteases.
S.P. Ikonomova, A.J. Karlsson
BIOT 521 - 9:30
Membrane cholesterol and the adenosine A 2a receptor.
C. McGraw, K. Cooke, A.S. Robinson
9:50 Intermission
BIOT 522 - 10:10
Identification of functional group characteristics and
physicochemical properties of Pseudomonas sp. strain ADP
biofilm.
V. Henry, J.L. Jessop, T.L. Peeples
BIOT 523 - 10:30
Characterization of an engineered, ligand-induced, viral
membrane fusion protein.
M. Valverde, M. Bell, E.T. Boder
Disease & Biomedical Applications I
8:30 - 11:30AM, Opal
Presiding: C. Chen, L. Servoss
Control of Protein Quality Attributes
Presiding: V. Janakiraman, T. Whitehead
Sponsored by: BioMarin Pharmaceutical Inc.
BIOT 534 - 8:30
Raw material specification lifecycle management.
L. Miller, A. Fries
BIOT 527 - 8:50
Stem cell-based blood-brain barrier model for drug transport
studies.
J. Mantle, K.H. Lee
BIOT 535 - 8:50
Maximizing the value of commercial scale data: A case study.
G. Naugle
BIOT 528 - 9:10
Controlling differentiation of induced pluripotent stem cells into
neurons in well-defined, hydrogel-based microenvironments.
E. Ovadia, D.W. Colby, A.M. Kloxin
BIOT 529 - 9:30
Discovery and deconstruction of oscillations in the nitric oxide
stress network of Escherichia coli.
M. Brynildsen, J. Robinson
9:50 Intermission
BIOT 530 - 10:10
Elucidating core mechanisms of dormancy regulation in breast
cancer cells.
A. Das, A. Kumar, J. Preciado, A. Aksan, S. Azarin
BIOT 524 - 10:50
Analyzing the effects of glycosylation on the physicochemical
properties and biological activity of antibody Fc regions using
a model system.
T.J. Tolbert
BIOT 531 - 10:30
Impact of high extracellular lactate on breast cancer metabolism.
D. Odenwelder, A. Brodsky, S.W. Harcum
BIOT 525 - 11:10
Cyclic peptide mimics of Aβ binding domain on transthyretin.
X. Lu, R.M. Murphy
BIOT 532 - 10:50
Novel aminoglycoside-hydrogel based in-vitro models of tumor
dormancy, relapse and metastases: Phenotype specific drug
screening, discovery and delivery.
T. Grandhi, T. Potta, K. Rege
Upstream Processes
Presiding: S. Abraham, C. Agarabi
BIOT 526 - 8:30
N-terminal hypothesis for Alzheimer’s disease.
B. Murray, J. Lippens, D. Fabris, S. Li, G. Belfort
BIOT 533 - 11:10
Reprogramming the local lymph node environment to promote
myelin-specific tolerance.
L.H. Tostanoski, C. Jewell
69
Quality by Design
BIOT 538 - 8:30
Elucidating raw material variability in mammalian cell cultures
using high throughput small-scale models.
H. Barkhordarian, J. Huang, C. Goudar
BIOT 536 - 9:10
Analytical method evolution during biological product life cycle.
P. Lei, Q. Qin, R. Zhang, M. Washabaugh
BIOY 537 - 9:30
Regulatory and technical considerations
management.
S. Lute, C. Agarabi, K.A. Brorson
9:50 Intermission
for
life
cycle
BIOT 539 - 8:50
Effects of process parameters and medium components on
charge variant distribution of a human monoclonal antibody.
T. Falkman, A. Vitina, T. Bjorkman, L. Kärf
BIOT 540 - 9:10
Impact of media components and seed conditions on cell
culture productivity and quality attributes of a fusion protein.
J. Xu, Y. Wang, X. Xu, J. Tian, C. Huang, L. You, N. Qian, Z. Li
Thursday Morning
BIOT 541 - 9:30
Achieving cell growth control and increased specific productivity
in CHO cell cultures without impacting product quality.
T. Tharmalingam, B.D. Follstad, T. Munro, C. Goudar
9:50 Intermission
BIOT 542 - 10:10
Understanding and controlling monoclonal antibody charge
heterogeneity in cell culture.
Y. Huang, W.C. Yang, C. Kwiatkowski
BIOT 543 - 10:30
Mechanistic elucidation of Fc glycan high mannose modulation
and its application for product attribute control.
J. Huang, P. Slade, S. Gupta, I. Liu, C. Zupke, G. Nyberg
BIOT 544 - 10:50
Effects of induction strategies on heterologous protein
expression and virus-like particle assembly in Pichia pastoris.
B. Blaha, T. Mukhopadhyay
BIOT 545 - 11:10
End-to-end approach to monitoring and reducing LMW
Formation during mAb process development.
A. Cura, R. Maurer, A. Shupe, S. Chollangi, K. Sing, K.
McWade, J. Yee, J. Ray, M. Peck, M. Lu, R. Martin, Y. Li, Z. Li
70
A
B
Upstream Processes
General Topics in Upstream Processes
Papers 546 - 553
2:00 - 5:00 PM
Crystal II
Presiding: C. Beisel, T. Tharmalingam
Non-Chromatographic Separations and Process
Integration II
Papers 554 - 561
2:00 - 5:00 PM
Emerald Ballroom
Presiding: H. Ahmandian, A. Lenhoff
C
Processes
Papers 562 - 569
2:00 - 5:00 PM
Diamond I
Prediction and Characterization of Biophysical
Properties
D
71
Disease and Biomedical Applications II
Presiding: N. Gupta, C. Heldt
General Topics in Upstream Processes
Presiding: C. Beisel, T. Tharmalingam
Papers 570 - 577
2:00 - 5:00 PM
Opal
Non-Chromatographic Separations & Process
Integration II
Presiding: H. Ahmandian, A. Lenhoff
BIOT 546 - 2:00
Design, analysis and assessment of novel enzymatic reactions
and biopathways.
N. Hadadi, M. Ataman, J. Hafner, V. Hatzimanikatis
BIOT 554 - 2:00
Salt tolerant tangential flow ultrafiltration membranes for
protein fractionation.
A. Arunkumar, M.R. Etzel
BIOT 547 - 2:20
Transcriptomics-guided design of regulatory elements: A
workflow to create synthetic promoters for mammalian hosts.
J.K. Cheng, H.S. Alper
BIOT 555 - 2:20
Production of stable high concentration monoclonal antibody
solutions with low viscosity by tangential flow ultrafiltration.
J. Hung, A. Borwankar, B. Dear, T. Truskett, K.P. Johnston
BIOT 548 - 2:40
Guiding rational glycan assay development using observability
analysis.
D. Radhakrishnan, A.S. Robinson, B. Ogunnaike
BIOT 556 - 2:40
Continuous processing for diafiltration operations in
biomanufacturing.
E. Peterson, H. Lutz
BIOT 549 - 3:00
Mechanistic understanding of chromosomal rearrangements
in CHO cells.
J. Baik, K.H. Lee
BIOT 557 - 3:00
Development of chiral membranes for purifying chiral feed
streams.
J.M. Imbrogno, V.L. Schultz, R.J. Linhardt, G. Belfort
3:20 Intermission
3:20 Intermission
BIOT 550 - 3:40
Evaluation of oxidative stress on cell metabolism and product
quality.
M. Handlogten, M. Zhu, S. Ahuja
BIOT 558 - 3:40
Enhancement of viral filtration performance by
characterization of the foulant particulates and controlling
factors contributing to sub-visible particle formation.
J. Woo, R. Emery, C. Garcia, N. Sanaie
BIOT 551 - 4:00
Evaluation of advanced media / feed characterization methods
for an improved understanding of media / feed variability.
S. Tummala, T. Webster, R. Beri
Presiding: B. Hackel, F. He
Upstream Processes
BIOT 552 - 4:20
Poloxamer: Steps towards cell culture media consistency.
J. von Hagen, S. Charaniya
BIOT 553 - 4:40
Semicontinuous bioreactor production of a recombinant
therapeutic protein in metabolically regulated transgenic rice
cell cultures.
J. Corbin, B.I. Hashimoto, K. Karuppanan, Z.R. Kyser, R.L.
Rodriguez, S. Nandi, B.A. Roberts, A.R. Noe, K. McDonald
Thursday Afternoon
Thursday Afternoon
BIOT 559 - 4:00
Pre-filtration and process improvements: Enhancing virus filter
performance by pre-filtration with surface modified membrane.
B. Cacace, S. Giglia, P.M. Goddard, H. Bak, C. Cowan, C.
Passno
BIOT 560 - 4:20
Effects of filtration condition on virus clearance.
R. Te, S. Eswaranandam, R. Wickramasinghe, X. Qian
BIOT 561 - 4:40
Understanding the impacts of viral clearance artifacts in
nanofiltration.
A.H. Schwartz
72
Prediction & Characterization of Biophysical
Properties
Presiding: B. Hackel, F. He
BIOT 562 - 2:00
Withdrawn
BIOT 563 - 2:20
Unfolding thermodynamics and β-sheet formation of helical
peptides.
C. Calero-Rubio, B.A. Paik, X. Jia, K.L. Kiick, C.J. Roberts
Thursday Afternoon
BIOT 564 - 2:40
Protein conformational array as a process development tool
for antibody higher order structure analysis.
D. Yu, Y. Song, E. Schutsky, M. Mayani, A.T. Lewandowski, Z.
Li
BIOT 565 - 3:00
Measurements of antibody self-association using affinitycapture self-interaction nanoparticle spectroscopy are
correlated with complex biophysical properties.
S.B. Geng, M. Wittekind, A. Vigil, P.M. Tessier
3:20 Intermission
BIOT 566 - 3:40
Microrheology and intrinsic viscosities of antibodies.
L. Josephson, D.L. Leiske, W.J. Galush, E.M. Furst
BIOT 567 - 4:00
Understanding of structural factors for enzyme activity and
stability and application to simultaneous enhancement of
enzyme activity and organic solvent stability.
Y.J. Yoo, C.F. Yagonia, H. Lee, S. Kang
BIOT 568 - 4:20
What effect does cleaning and sanitization in place have on
protein affinity ligands?
M. Wetterhall, E. Monie, A. Mattsson, A. Gronberg, G.
Rodrigo, T. Bjorkman
BIOT 569 - 4:40
Structural flexibility, hydrophobicity and charge regulation
in histidine-rich antimicrobial piscidins underpin membrane
disruption examined in real time.
M. Sorci, J. Seckute, N. Smajic, S.B. Perrin, L.K. Nicholson, J.
Blazyk, R. Pastor, M. Cotten, G. Belfort
73
Disease & Biomedical Applications II
2:00 - 5:00PM, Opal
Presiding: N. Gupta, C. Heldt
The ACS Biochemical Technology Division
gratefully acknowledges the 2016 financial
support provided by these biotechnology
industry leaders.
BIOT 570 - 2:00
Fourier transform infrared spectroscopic spectral feature
subset selection for optimal diagnosis of oral lesion.
S. Banerjee, J. Chakraborty, M. Pal, R. Paul, J. Chatterjee
BIOT 571 - 2:20
Highly sensitive smartphone-based point-of-care diagnostics
enabled by persistent luminescence nanoparticles.
A. Paterson, B. Raja, G. Garvey, E. Finley, J. Brgoch, R.C.
Willson
BIOT 572 - 2:40
Paper microfluidics for the detection of infectious diseases.
J. Trabuco, D.M. Prazeres, R.C. Willson
BIOT 573 - 3:00
New method of decreasing fouling and increasing gas transfer
of polysulfone hollow-fiber membranes using peptoids.
N. Mahmoudi, M. Asgharpour, L. Reed, J. Hestekin, S.L.
Servoss
3:20 Intermission
BIOT 574 - 3:40
Therapeutic probiotics engineered to target colorectal cancer.
H.C. Loong, M. Chang
BIOT 575 - 4:00
Rapid uptake of fluorescent peptides into intact mammalian
cells using a β-Hairpin sequence motif.
A.T. Melvin
BIOT 576 - 4:20
Connectosomes for direct intracellular drug delivery.
A. Gadok, D. Busch, J. Stachowiak
BIOT 577 - 4:40
Importin-4 regulates gene delivery by enhancing nuclear
retention and chromatin deposition by polyplexes.
M.O. Sullivan, E. Munsell, N.L. Ross
74
SE M
M AR
IN C
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0– H
2 E
P.
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Alexion proudly supports the BIOT Division of the ACS
Alexion is a biopharmaceutical company focused on serving patients with severe and rare disorders through the
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in complement inhibition and has developed and markets Soliris® (eculizumab) as a treatment for patients with PNH
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GE, GE monogram, and ÄKTA are trademarks of General Electric Company.
© 2015–2016 General Electric Company. First published Oct. 2015.
GE Healthcare Bio-Sciences AB, Björkgatan 30, 751 84 Uppsala, Sweden
For local office contact information, visit www.gelifesciences.com/contact
29167615 AA 01/2016
alexionpharma.com
78
79
80
Index
A
Abadie, K....................................173
Abnouf, S.....................................93
Abu-Absi, N.R...........................292
Ackerman, M..............................417
Ackerman, S.E..........................438
Adams, M.W...............................161
Adeniran, A..................................24
Adhikari, M.................................346
Agarabi, C.........................294, 537
Agastin, S.....................................41
Aguirre, J....................................434
Ahmadian, H.................. ..345, 396
Ahmed, Z...................................248
Ahnfelt, M...................................158
Ahuja, S.....................................550
Akhigbe, F.U..............................383
Akkas, T......................................381
Aksan, A.....................................530
Al Rowaihi, I.S...........................403
Alexander, S.C...........................183
Alford, R.....................................353
Allen, C.D...................................136
Allen, M.A..................................318
Alonso Guitierrez, J....................29
Alper, H.S..........................204, 547
Alter, G........................................417
Alters, S........................................89
Altshuler, R................................457
Alving, C.....................................295
Amrhein, N.................................124
Ander, M.....................................406
Anderko, A.M.............................395
Andersen, G..............................345
Anderson, J.......................238, 239
Anderson, R...............................401
Andersson, C.............................157
Andes, D....................................362
Andreozzi, S.......................107, 196
Angelo, J.M................................429
Anthony, L..................................144
Antoline, J..................................295
Antoniewicz, M.R.......................101
Ardejani, M.S.............................138
Arha, M.......................................316
Arkin, A.P.....................................60
Armando, J.....................................7
Armiger, W.B..............................471
Aron, K........................80, 248, 476
Arunkumar, A...............42, 81, 342,
............................................387, 554
81
Arvay, E......................................266
Asensio, M..................................113
Asgharpour, M...........................573
Asher, D........................................38
Ashton, R....................................151
Ataman, M.................................546
Atsumi, S....................................366
Attuah, G....................................376
Aucamp, J..................................450
Aucoin, H.R................................274
Auxenfans, T..............................143
Avalos, J....................................464
Averick, S...................................352
Averick, S...................................436
Avila, A.......................................319
Aysola, M......................................15
Ayturk, E..........................6, 49, 400
Ayyadurai, N..............................296
Azarin, S....................................530
B
Badur, M.......................................73
Baganz, F.....................................43
Baik, J.........................................549
Bak, H.........................................559
Baker, J.C...................................191
Baker, P......................................360
Bakhshayeshi, M.......................169
Banaszak Holl M.M...................317
Bandi, S......................................462
Banerjee, S...................71,457, 570
Banta, S....................142, 486, 487
Baragan, L.................................389
Barata, T.S.................................224
Barker, G....................................430
Barkhordarian, H.......................538
Barringer, G..................................78
Barua, S.....................................273
Bassalo, M.................................210
Basser, P.J.................................314
Bauer, K.C.................................356
Bauren, G..................................406
Beard, S.....................................324
Becerra-Arteaga, A............87, 390
Bechinger, B..............................303
Becker, S....................................124
Beckham, G...............................290
Begum, Z...................................453
Beisel, C............................209, 494
Belfort, G............94, 526, 557, 569
Bell, C.........................................238
Bhosale, P..................................124
Bianchi, D.......................................7
Biddy, M.....................................290
Bill, J...................................277, 499
Binabaji, E..................................426
Bisschops, M.............................394
Bisschops, M...............................47
Bjorkman, T..............172, 347, 406,
...........................................539, 568
Black, B......................................290
Blackburn, E...............................124
Blackstock, D.............................493
Blaha, B.....................................544
Blanck, E...................................249
Blando, K....................................194
Blazyk, J.....................................569
Blenner, M.A.......96,164, 266, 360
Blocker, K.M.................................64
Blomqvist, L.................................76
Bobay, B.G.................................370
Boder, E.T............................56, 523
Bodner, K....................................121
Boesch, A...................................417
Boeth, D......................................192
Bogorad, I.W..............................465
Bolisetty, P.................................293
Bolton, G.....................................169
Bonk, B.......................................468
Boppidi, K...................................199
Borrajo, J....................................277
Boruah, N...................................425
Borwankar, A...........226, 476, 555
Borys, M.C.........80, 248, 292, 476
Botero Besada-Lombana, P....289
Bothof, C.....................................130
Boutom, S...................................317
Boyle, N.R..........................152, 274
Bozekowski, J............................312
Bracewell, D.G....43, 86, 322, 359
Bradfield, M...............................290
Bramson, J.....................................3
Brantley, M.................................280
Brantley, T..................................391
Brass, L......................................388
Brazel, C.S................................252
Breil, M.P....................................427
Brekkan, E..................................158
Brestrich, N................................133
Brgoch, J....................................571
Briggs, J.....................................252
Brink, C......................................364
Brinkmann, A....................251, 477
Brisendine, J..............................486
Britton, J.....................................469
Broadbelt, L.J.............................106
Broberg Hansen, E...................427
Brocchini, S...............................224
Brodean, E................................264
Brodsky, A..................................531
Brogan, A...................................458
Brorson, K.A..............37, 294, 329,
............................................337, 537
Brower, K...................................495
Brower, M.....................................82
Brown, A....................................485
Brown, D.....................................124
Brown, M...........................329, 337
Bruce, M...................................9, 11
Brunk, E.C..........................112, 419
Brunson, J..................................124
Brynildsen, M............................529
Buckley, J.J..................................16
Bulpin, M.......................................15
Bulutoglu, B.......................142, 487
Bunck, D............................185, 358
Bunnell, B...................................228
Burg, J.........................................126
Burgard, A.P...............................196
Burkart, M.D..............................506
Busch, D.....................................576
Busson, K..........................343, 372
Butler, M......................................173
Bystroff, C..................................457
Byun, S.......................................284
C
Cabrera, J..................................253
Cacace, B..................................559
Calero-Rubio, C.......221, 223, 563
Calzada, J..................................430
Cangelosi, D..............................447
Cao, B........................................448
Cao, J.................................202, 316
Cao, M........................................165
Carbonell, R.G..................170, 370
Cardenas, J...............................200
Caron, J............................9, 11, 511
Carredano, E.............................372
Carta, G.....................................430
Casada, S...................................124
Case, B.........................................17
Castan, A.............................76,242
Cea, M........................................378
Cecchini, D........................169, 300
Cha, H.J............55, 229, 256, 296,
.....................................308,313 368
Chabbert, B................................143
Chai, Q........................................180
Chakrabarti, A...................196, 402
Chakraborty, J...........................570
Chan, D......................................457
Chandarana, N..........................252
Chandra, D................311, 334, 392
Chandran, S...............................207
Chang, M............................190, 574
Charlesworth, A..........................95
Chase, C.....................................171
Chase, M....................................124
Chatterjee, J..............................570
Chau, D......................................233
Chavez, B..................................294
Chelius, C...................................199
Chen, C.............181, 421, 423, 465
Chen, H.....................247, 309, 310
Chen, J.......................................317
Chen, L.........................................97
Chen, M.....................................505
Chen, Q.......................................173
Chen, R.P...................................493
Chen, W..................27, 31, 97, 114,
............................................493, 512
Chen, Y........................................113
Chen, Y........................................181
Chen, Z.........................................51
Cheng, J.K.................................547
Cheruzel, L.E............................389
Cheung, W.L..............................505
Chi, W..........................................181
Chiang, Y......................................43
Cho, Y..........................................141
Choi, A........................................457
Choi, B........................................229
Choi, B..........................................55
Choi, J........................................254
Choi, Y........................................275
Choi, Y........................................296
Choi, Y........................................308
Chollangi, S................39, 514, 545
Christensen, M....................34, 273
Chu, T..........................................417
Chumsae, C.................................36
Chun, G.............................283, 284
Chung, W...................................384
Chuong, T...................................441
Cilliers, C....................................481
Clark, N.......................................124
Cochran, J...........................91, 438
Cochran, K.................................137
Coffman, J.L.................5, 350, 414
Cohen, Y....................................448
Colak Atan, S...............................83
Colby, D.W.................................528
Coleman, G...............................425
Collins, C.H.........................58, 125
Collins, M........................................6
Collins, S.M.................................62
Columna, K...............................354
Conley, L...........................300, 391
Constantine, B...........................182
Cook, J.........................................95
Cook, S.........................................14
Cooke, K.....................................521
Coolbaugh, M.J.........................137
Cooper, A.J................................106
Cooper, C....................................111
Copeland, M...............................211
Coppock, M................................447
Corbin, J.....................................553
Corckran, J.G.............................197
Cotten, M...................................569
Coufal, M...................................452
Counts, J.....................................118
Cowan, C...................................559
Cox, M........................................306
Cox, N.........................................438
Cox, V.........................................270
Crabbe, E...................................285
Cramer, S.....................................89
Cramer, S.M......71, 311, 333, 334,
.................336, 345, 348, 392, 396
Creasy, A...................................430
Cress, B.....................................384
Croissant, J.G...........................279
Croughan, W.D..........................292
Culler, S.J..................................206
Culver, J.N.................................485
Cummings, C.............................437
Cundy, J...............................14, 497
Cunningham, K........................9, 11
Cura, A.......................................545
Currier, N.V................................438
Cutler, C.......................................82
D
Da Silva, N.A....................200, 289
Dalal, N.G..................................285
Dalby, P......................................244
Dandekar, A.M..................144, 215
Daniels, W..................................128
Dantas, G....................................98
Daris, K........................................77
Das, A........................................530
82
Das, S.......................358, 447, 492
Daugherty, P.S..................312, 415
Davis, R.W..................................100
de los Reyes, G...........................85
Dear, B...............................226, 555
DeChiara, J................................321
Deily, C......................................306
DeLisa, M.P.......................109, 139
DeLorenzo, D.............................166
Deng, L.......................................279
Deorkar, N..................................306
DePorter, S.M............................438
Derda, R.....................................440
Desai, J.......................................118
Desai, S.....................................366
Deutsch, S.................................509
Devaraj, N.K...............................183
Dey, M........................................454
Deyle, K......................................185
Dhamane, S.P...........................346
Dhanasekharan, K...........194, 324
Dhingra, K..................................187
Diamond, S................................388
Dickinson, C.D...........................180
Diep, C.......................................319
Dietrich, K..................................403
DiGiovanni, J...............................89
Dimitiadis, E.K...........................314
Dimov, N.......................................43
Ding, D.......................................380
Ding, W.......................................301
Discenza, J................................285
Divakaruni, A...............................73
Doane, A......................................40
Dodds, D.R.................................471
Doninger, K................................156
Donnelly, J.................................355
Dooley, K....................................142
Dordick, J.S...............457, 471, 516
Dowe, N.....................................290
Du, C..........................................476
Dubin, P.L...................................513
Duhra-Gill, S..............................215
Dukleska, S...............................340
Dumetz, A..................................374
Dumetz, A.C..............................330
Dupont, A...................................245
Duran, N.....................................319
Durie, D......................................335
E
Easson, J...................................288
Eaton, S.....................................273
83
Edman Orlefors, A....................364
Edwards, A................................210
Egan, S.......................................476
Ehamparanathan, V..................476
Eklind, I.........................................33
Elich, T........................................128
Elmer, J......................................273
Elouafiq, S..................................251
Elsner, M.....................................475
Emery, R....................................558
Emmerson, G..............................78
Endalur Gopinarayanan, V.........30
Eppinger, J..........................117, 403
Eppink, M...................................386
Ercek, D.T....................................113
Erickson, J..................................451
Eriksson, K.O....................331, 343
Eslamimanesh, A......................395
Eswaranandam, S....................560
Etzel, M.R..................................554
Evans, W....................................402
F
Fabris, D..............................94, 526
Fahrner, R....................................46
Falatach, R................................352
Fäldt, E.........................................76
Falk, B.W.....................................144
Falkman, T...................76, 347, 539
Fan, C..........................................181
Fan, Y..........................................137
Fang, Z.......................................301
Farinas, C...................................148
Farrow, B....................................185
Farutin, V....................................239
Feist, A.......................................424
Feizi, A........................................413
Fenard, D...................................303
Feng, L.........................................23
Feng, Y........................................374
Fengos, G...................................418
Ferreira, G.........................323, 502
Fiadeiro, M...................................46
Fieger, M....................................288
Fiehn, O......................................106
Field, N...............................154, 327
Filsinger Interrante, M...............201
Finley, E......................................571
Fisher, S.............................173, 499
Fisk, J.........................................456
Follstad, B.D..............................541
Forss, A........................................76
Foster, K.....................................153
Foston, M.......................................98
Foston, M.B................................149
Fox, S...........................................39
Francesko, A.............................484
Frank, A.......................................117
Franklin, J...................................168
Fraser, K.....................................457
Frech, C......................................217
Frederiksen, S.S.......................427
Frelin, O......................................106
Fries, A.......................................534
Frymier, P.....................................56
Fu, L............................................516
Fujita, Y......................................395
Furst, E.M.........................223, 566
G
Gadok, A....................................576
Galanie, S..................................201
Galipeau, K.................................511
Galm, U.......................................124
Galush, W.J...............................566
Galy, A........................................303
Gambill, L.....................................96
Gantier, R...................6, 47, 49,394
Gao, M........................................165
Garcia, C....................................558
Garcia, K....................................487
Garde, S.......................................71
Gardner, J...................................152
Garrison, R.................................124
Garst, A.D..................................210
Garvey, G...................................571
Garvin, C...................................500
Gastfriend, B.D..........................197
Gates, A......................................151
Gaucher, E.................................270
Gawlitzek, M..............................307
Ge, X...........................................175
Geng, S.B...................................180
Geng, S.B..................................565
George, K...................................419
Georgiou, G.................................89
Gerberich, C.....................330, 374
Gercke, J.C..................................39
Gerepka, S.................................277
Gerontas, S................................431
Gervais, J...................................216
Ghose, S....................................300
Ghosh, D....................................405
Ghosh, R....................................221
Ghosh, R..................................3, 48
Gibson, E.R...............................263
Giglia, S............................335, 559
Gill, R.T.......................................210
Gillespie, C........9, 11, 38, 131, 511
Gillespie, R.................................167
Gilmore, S....................................59
Gilmore, S.P........................32, 159
Gjoka, X...............................47, 394
Glajch, J.....................................239
Glasgow, J..................................113
Glattard, E..................................303
Gliddon, H....................................21
Göbel, M....................................356
Goddard, P.M............................559
Gokhale, M................................301
Goklen, K.E...............243, 374, 474
Goldfeld, M................................244
Gonzalez, J..........................31, 101
Gonzalez, M.E...........................378
Gonzalez-Malerva, L................273
Good, M.R.................................244
Goodrich, E.M...........................517
Goodwine, C.....................311, 392
Goodwine, C.A.........................334
Gopalakrishnan, S........................2
Gorrell, J.............................168, 173
Gospodarek, A............................82
Goudar, C.........421, 423, 538, 541
Gowtham, Y..................................75
Goyal, G.......................................29
Graalfs, H....................................131
Graham, H.................................285
Grandhi, T..................................532
Grant, G.A.................................438
Gray, M......................................460
Greene, D.G...............................131
Griesbach, J......................156, 432
Griesbraber, G....................83, 130
Grigoriev, I..................................102
Grisewood, M..............................92
Groetzinger, S....................117, 403
Groll, M........................................117
Gronberg, A...............172, 364,568
Gronke, R.S................................193
Grosskopf, V..............................307
Grundy, L...................................464
Gruver, R....................................265
Guan, Z......................................278
Gude, V..............................145, 408
Guo, J.........................................184
Guo, S.........................................271
Gupta, A...............................87, 517
Gupta, R.K.................................405
Gupta, S.....................................543
Gutierrez Bugarin, J.M..............413
Guzman, B..................................171
G
Habel, J......................................344
Haberman, P..................................7
Hackel, B..............................17, 177
Hadadi, N...................................546
Hadidi, M.......................................16
Haeringer, K...............................158
Hafner, J.....................................546
Hagstrom, A.....................346, 502
Hahn, J.........................................62
Hahn, T.......................................432
Haley, E.M.................................232
Hall, M........................................343
Hallett, J.P..................................458
Hamaker, K..................................39
Hamilton, R.................................121
Han, I..........................................340
Han, T..................................45, 304
Han, X........................................379
Hanamura, M.............................363
Handlogten, M...........................550
Hanke, A....................................386
Hanson, A..................................106
Hanson, C.D..............................255
Hansson, J.U.............................398
Harcum, S.W.......................75, 531
Harlen, O....................................382
Harris, H.W...................................63
Harris, S.............................199, 382
Hasegawa, S..............................155
Hashimoto, B.I...........................553
Hashimoto, Y..............................170
Hassanpour, N...........................110
Hassoun, S..........................65, 110
Hatch, P........................................38
Hatzimanikatis, V.....107, 196, 208,
............................................418, 546
Hauk, P.......................................422
Hawkins, A.................................161
Haynes, K..................................273
He, D...........................................286
He, N..........................................367
Heath, J......................................358
Heath, J.R........185, 358, 447, 492
Heinen-Kreuzig, A.....................515
Heldin, E.....................................172
Heldt, C..................................12, 22
Heller, M.....................................254
Henry, C.....................................106
Henry, V......................................522
Henske, J..............32, 59, 102, 159
Henson, W.R......................98, 445
Heo, H........................................313
Hepbildikler, S...................158, 432
Hernandez, F.E.........................355
Hernandez-Lozada, N................92
Hershewe, J...............................123
Hershey, N.................................476
Herzer, S...........................293, 430
Hestekin, J.................................573
Hester, J.F....................................81
Higami, M..................................363
Hill, K...........................................124
Hiller, K.........................................73
Hillson, N......................................29
Hitzler, J.....................................450
Ho, H...........................................281
Hoang, H....................................213
Hodge, B.....................................152
Hodgman, E.................................41
Hoener, B..................................404
Hoggard, A................................404
Holec, P.V...................................177
Hollenbach, M................................1
Holmqvist, A......................134, 157
Honda, H.........241, 260, 262, 276,
..........................281, 282, 298, 299
Hong, S......................................267
Hood, T.........................................15
Hoover, C...................................509
Horava, S...................................222
Horkay, F....................................314
Horkayne Szakaly, I..................314
Horvath, N..................................109
Hoshan, L..........................247, 250
Hossler, P.....................................36
Howes, P.D...................................21
Hoynes-O’Connor, A........166, 445
Hsu, C...........................................37
Hu, R.........................309, 310, 369
Hu, W...................................40, 233
Hua, X...........................................51
Huang, C..................361, 455, 540
Huang, J...................296, 538, 543
Huang, Y............................476, 542
Huang, Z.......................................79
Hubbuch, J..............133, 220, 356,
...........................................432, 562
Huffman, B..........................14, 497
Hung, J......................................226
Hung, J.......................................555
Hunt, S.M...................................291
Hunter, A....................................498
Hutcheson, S.W.........................197
Hutchins, S..................................80
84
Hutchinson, M............................168
Huuk, T.......................................432
Huxford, T..................................377
Hwang, B.........229, 256, 296, 308
Hwang, C........................................1
Hwang, D.....................................55
Hwang, I......................................190
Hwang, M...................................144
I
Ibrahim, B.B..............................399
Ibwe, E........................................124
Ijeomah, B..................................118
Ikonomova, S.P.........................520
Imbrogno, J.M...........................557
Immethun, C...............................166
Insaidoo, F.K................................70
Isakari, Y.....................................155
Iskra, T........................................128
Islam, T........................................170
Ismail, Z.Z..................................365
Itoh, D..........................................155
Iwo, G.........................................376
J
Jacob, J......................................328
Jacobson, A..............................295
Jacquemart, R..........................214
Jaeger, C......................................73
Jain, A.........................................178
Jain, H..........................................37
Jain, S................................174, 338
Jakobson, C...............................113
Jalah, R......................................295
Jan, C.D.......................................39
Jaquez, O...................................193
Jaycey, H....................................153
Jeffryes, J............................66, 106
Jellum, G.M..................................74
Jeon, E......................................229
Jessop, J.L................................522
Jeugt, B......................................326
Jewell, C.....................................533
Jewett, M..............4, 123, 439, 442
Jewett, M.C................................109
Jia, H.............................................51
Jia, X..........................................563
Jiang, B.............................310, 369
Jiang, J.......................................231
Jiao, Y.........................63, 108, 395
Jin, Y............................................147
Johansson, H.J.........................385
85
Johansson, K.............................427
Johnson, C.................................445
Johnson, D.E...............................89
Johnson, S.................................337
Johnson, T.................................359
Johnston, K.P...................226, 555
Jones Prather, K.L....................468
Jones, H......................................124
Jones, J.......................................124
Jones, J.A...................62, 287, 384
Jones, K.....................................491
Jones, V.....................................457
Josephson, L............................566
Joyce, J......................................412
Joyce, J.G..................................328
Julian, M.............................140, 297
Jung, D..............................308, 368
Jung, G........................99, 229, 272
Jung, S.......................................393
Jurlewicz, K.......................154, 351
K
Kaar, J..........................................52
Kaforou, M...................................21
Kalbarczyk, K.Z..........................58
Kalbfuss-Zimmermann, B........375
Kaligotla, H................................349
Kallick, E....................................436
Kaltenbrunner, O......................452
Kamat, S....................................195
Kanani, D.M...............................214
Kane, R......................................316
Kane, R.S...................................471
Kang, S......................................567
Kantardjieff, A...........................353
Kao, C.........................................231
Kappel, H....................................417
Karadeema, R.J.......................257
Karande, P..........53, 311,334, 336,
............................................392, 490
Kärf, L...............172, 347, 364, 539
Karkov, H..........................345, 396
Karlsson, A.J....................489, 520
Karuppanan, K..................215, 553
Kastantin, M.................................52
Kato, M.......................................389
Kaur, S........................................319
Kawka, K....................................478
Kay, J..........................................442
Kazan, G....................................335
Kazemi, A.S...............................478
Ke, N...........................................139
Keasling, J.D.......................29, 419
Kehoe, D.......................................15
Keller, M.W..................................161
Kelley, W.....................................187
Kelly, E.E....................................125
Kelly, R.L....................................483
Kelly, R.M...........................118, 161
Kelly, W.J......................................79
Kett, W..........................................44
Ketterer, P...................................124
Khan, A........................................68
Khashab, N.M............................279
Khoo, S......................................496
Kiesewetter, A....................129, 515
Kiick, K.L....................................563
Kikuchi, N......................................18
Kim, C................................256, 313
Kim, D........................................384
Kim, E..........................21, 113, 284
Kim, J................................259, 509
Kim, M........................................259
Kim, S..................................98, 269
Kim, S........................................284
Kim, S........................................284
Kim, Y........................148, 232, 284
Kind, T.........................................106
King, J..........................................22
King, J............................................7
Kini, A.........................................320
Kintzing, J.R..............................438
Kinzlmaier, D..............................517
Kish, W.S...................................370
Kisley, L.....................................404
Klesmith, J...................................28
Kloxin, A.M.......................234, 528
Kluters, S...................................217
Ko, W..........................................268
Koder, R.L.................................486
Koffas, M..............58, 62, 384, 471
Kolpashchikov, D..........................18
Kong, S.......................................431
Konietzko, J......................328, 412
Konkolewicz, D.................352, 480
Konstantinidis, S.......154, 327, 351
Koo, B.........................................444
Koppejan, V................................323
Kourentzi, K...............................346
Kraft, J........................................146
Krajcovic, M........................80, 476
Kral, P.........................................410
Kratzer, A...................................280
Krishnan, R...................................14
Kristopeit, A................................412
Krogh, B.O.................................396
Kruh, J.........................................118
Krühne, U.....................................43
Kruziki, M......................................17
Ku, S............................................153
Kublbleck, J.A..............................46
Kuhn, B.......................................452
Kulkarni, S..................................271
Kumar, A....................................530
Kumar, S.......................................72
Kumar, V.....................................132
Kwak, J.........................................53
Kwiatkowski, C...........................542
Kyser, Z.R..................................553
L
LaBaer, J....................................273
Labanca, M................................300
Lachance, D.M............................62
Lacki, K.......................................158
Ladisch, M.R.....................148, 153
Ladiwala, A.................................172
Lagos, J......................................248
Laha, A.......................................225
Lahiry, A......................................122
Laird, M.......................................121
Lakkyreddy, J..............................35
Lamberson, C...........................457
Landes, C.F.......................130, 404
Landes, C.F...............................404
Lane, T........................................100
Langford, T.F................................19
Larsen, T...........................433, 452
Larsson, O.................................242
Laslop, A.....................................411
Latulippe, D.R............................478
Lawson, T.....................................15
Le, D...........................................483
Le, H....................................421,423
Le, K..............................................77
Le, R.............................................56
Lease, R.....................................122
Lebrilla, C...................................215
Ledra, N.....................................253
Lee, A...........................................33
Lee, A.G.....................................292
Lee, B.........................................284
Lee, C................................140, 297
Lee, D........................................284
Lee, H.......................268, 269, 567
Lee, K.H.............................527, 549
Lee, M........................................362
Lee, S..........................................120
Lee, S.........................................284
Lee, T..........................29, 198, 419
Lee, Y.........................................445
Lee, Y.F.......................................217
Leenay, R..................................209
Lehrman, J.................................273
Lei, P...........................................536
Leidel, C..........................................7
Leiske, D.L.................................566
Leitz, Q......................................384
Lenhoff, A.M...............69, 131, 429
Lerma-Ortiz, C...........................106
Lester, P......................................173
Levin, M........................................21
Levy, N.E....................................243
Lewandowski, A.T............212, 342,
............................................476, 564
Lewer, P.......................................124
Lewis, A.....................................292
Lewis, N.E.................203, 413, 420
Li, D..............................................141
Li, F.............................................295
Li, H............................................340
Li, H...............................................70
Li, J.....................................301, 439
Li, L.............................................416
Li, M............................................150
Li, N.............................................146
Li, Q.............................................184
Li, S............................199, 436, 526
Li, T................................................67
Li, X.............................................153
Li, X...............................................79
Li, Y................................................13
Li, Y.............................................286
Li, Y................................................51
Li, Y..............................................514
Li, Y.............................................545
Li, Z.............................................212
Li, Z.............................................248
Li, Z.............................................292
Li, Z.............................................342
Li, Z.............................................361
Li, Z.............................................455
Li, Z.............................................467
Li, Z.............................................476
Li, Z.............................................540
Li, Z............................................545
Li, Z............................................564
Li, Z...............................................80
Lian, I............................................20
Liang, J.......................................358
Liao, J.........................................187
Liao, J.C.....................................465
Lim, C...........................................55
Lim, H.........................................272
Lim, H...........................................99
Lin, C...........................................181
Lin, J............................................116
Lin, X...........................................168
Lind, O........................................331
Linden, T.O................................329
Ling, W.......................................244
Linhardt, R.J.............384, 516, 557
Link, A................................136, 505
Linke, T.......................................498
Lintern, K......................................86
Linville, T....................................252
Liou, J........................................222
Lippens, J............................94, 526
Lipscomb, G.L............................161
Liu, I............................................543
Liu, S..........................................335
Liu, X..........................................153
Liu, Z.............................................84
Ljunglöf, A..................172, 331, 347
Lo, Y...................20, 241, 260, 262,
...................................276, 282, 298
Loder, A.J...................................161
Loebs, A.............................164, 466
Loong, H.C.................................574
Looze, B.....................................500
Lopez, C.....................................165
Lopez, T.J...................................175
Louie, K......................................509
Lu, H............................................181
Lu, M...........................................476
Lu, M..........................................545
Lu, Q..........................................296
Lu, T...........................................202
Lu, X...........................................525
Lucini, C.......................................41
Lucius, M....................................480
Lucks, J.B..................................446
Lundgren, M..............................249
Lundin, V....................................463
Luo, M........................................494
Lute, S.......................329, 337, 537
Luther, M....................................353
Luther, S.......................................15
Lutocka, A..................................124
Lutz, H.......................................556
Lutz, N.......................................515
Lynch, M....................126, 482, 508
Lynch, S.....................................210
Lynn, D.M..................................362
Lyubarskaya, Y...........................193
M
86
Ma, G..........................................374
Ma, H............................................20
Ma, H............................................57
Ma, J.........................310, 369, 426
Ma, L..........................................286
Madadkar, P.................................48
Maddipati, P................................124
Madhavan, L..............................330
Madsen, S..................................228
Magarian, N................................173
Mageeney, C.............................280
Magill, A.....................................501
Magill, G.....................................173
Magilton, W...............................280
Magnusson, A...........................249
Magnusson, F.............................134
Mahan, A....................................417
Mahmoudi, N.............................573
Mahmud, T.................................503
Majewska, N..................................4
Majumdar, S..............................225
Maksimchuk, K..........................209
Maksimov, M.............................505
Malik, W.....................................246
Mallela, K...................................462
Maloisel, J..................................331
Mammo, T.A..............................182
Manalis, S...................................519
Mangrolia, P...............................305
Mansell, T.....................................61
Mantle, J.....................................527
Mao, C........................................488
Maranas, C..................................67
Maranas, C..................................92
Marchillo, K...............................362
Marcun, L....................................124
Marcus, J......................................65
Markley, A.L...............................160
Marlow, M...................................315
Marquardt, L..............................248
Marques, B.F.............................474
Marques, M..................................43
Marruecos, D...............................52
Marsh, E.....................................317
Martel, R.....................................476
Marten, M...................................199
Martin, C....................................245
Martin, H....................................372
Martin, J........................................14
Martin, R........................................4
Martin, R....................................545
Martinez-Guerra, E..........145, 408
Marty, J.......................................124
Massicotte, R.C........................339
87
Matos, T.....................................345
Matsuda, T.................................363
Matthiesen, J.............................470
Mattsson, A.......................406, 568
Matuck, J......................................36
Matyas, G..................................295
Maurer, R...................................545
Mayani, M.................212, 476, 564
Mayfield, S.................................271
Maynard, J..................................179
McCaffrey, T..............................253
McCarthy, A..............................358
McCutcheon, D..........................491
McDermott, S..............................36
McDonald, K.............144, 215, 553
McDowell, T...............................377
McGlothlen, M.............................38
McGraw, C.................................521
McInnis, P..................................245
Mckay, R....................................422
McNaughton, B.........................460
McNulty, A.K..............................130
McWade, K................................545
Mehta, A.....................................247
Mehta, A............................250, 373
Mehta, K.............................171, 473
Mehta, S.....................................454
Meier, A......................................307
Meier, S......................................307
Meier, S........................................35
Meier, S.J...................................265
Melvin, A.T.........................443, 575
Menacho Melgar, R..................482
Menegatti, S...............88, 170, 370
Meneghini, L..............................461
Menstell, P.................................129
Mercaldi, M........................174, 338
Merzel, R.L................................317
Metallo, C.....................................73
Meuse, C.W...............................459
Meyer, C.R.................................319
Meyer, J......................................149
Migani, D....................................322
Mihranyan, A.............................518
Mikola, M.R................................124
Miller, B.......................................152
Miller, E.......................................124
Miller, J........................................414
Miller, L.......................................534
Minteer, S.D...............................487
Mire-Sluis, T..............................452
Miro-Quesada, G....449, 498, 502
Mishra, A....................................358
Miskovic, L.................107, 196, 418
Misra, A......................................425
Moatsou, D................................439
Moghaddam-Taaheri, P............489
Mohagheghi, A.........................290
Mohammed, A..........................365
Mohror, L....................................255
Molek, J.R.........................243, 330
Molino, N....................................479
Mollet, M....................................449
Monbouquette, H.......23, 258, 444
Monie, E............................406, 568
Monk, J.......................................112
Moon, T......................98, 166, 445
Moore, K........................................4
Morrison, C.J..............................128
Morrison, C.S.............................471
Moscariello, J.............................151
Moses, S....................................288
Moskovits, M..............................441
Mostafa, S.S..................................2
Mouncey, N................................124
Moy, K........................................222
Mrksich, M..................................123
Muchena, J.K............................215
Muench, S.P..............................382
Mukherjee, A.....................118, 316
Mukherjee, S.............................261
Mukhopadhyay, T......................544
Mulato, L....................................319
Mullerpatan, A...........................336
Mullin, L..............................9, 11, 38
Mun, M.......................................496
Munro, T.....................................541
Munro, T.......................................77
Munsell, E..................................577
Murphy, A.....................................73
Murphy, R.M.....................305, 525
Murray, B.............................94, 526
Murrell, J.......................................15
Myers, J....................238, 239, 472
N
Nag, A..............................358, 492
Nagaraju, S...............................162
Nagpal, K...................................373
Naik, A................................170, 370
Naim, A......................................277
Nair, N.U..............................65, 110
Najafi, H.......................................54
Nakamura, S.............................363
Nandi, S.............................215, 553
Narambuena, C........................429
Naresh, A..........................326, 363
Naugle, G...................................535
Navia, R......................................378
Navid, A...............................63, 108
Neek, M......................................479
Nelson, E.L................................479
Newton, J.M...............................357
Ng, A..........................................445
Ng, B...........................................476
Ng, K..................................166, 445
Nguyen, E..................................354
Nicholson, L.K...........................569
Nicole, W...................................290
Niehaus, T..................................106
Nikolov, Z.L................................271
Nilsson Välimaa, K....................364
Nilsson, B...................134, 157, 427
Niu, W.........................................184
Noe, A.R....................................553
Noh, M.................................99, 272
NorÃ©n, .................................B.331
Norling, L....................................173
North, H......................................152
Northen, T..................................509
Ntai, I..........................................439
Nwaichi, E.O..............................376
Nyberg, G..................................543
O
OConnor, K................................228
Odenwelder, D...........................531
Ogunnaike, B....................321, 548
Oh, T...........................................254
Okano, Y.....................................363
O’Keeffe, R................................475
Oldenhuis, N.J...........................278
Olsen, W....................................338
O’Malley, M.A.......32, 59, 159, 415
Omar, H.W.................................279
Omokehinde, T..........................318
O’Neill, K...........................247, 250
Opdam, S...................................420
O’Reilly, R.K..............................439
Ortiz, K.......................................233
Oruklu, S....................................500
Osei-Owusu, N...........................171
Ostermeier, M....................135, 422
Ottens, M..........................323, 386
Ovadia, E...................................528
Ozbakir, H.F...............................486
Ozturk, S.S.................................513
P
Paes, G......................................143
Page, R.C.........................352, 480
Paghdal, N.................................214
Paik, B.A....................................563
Pal, M.........................................570
Palecek, S.................................362
Paley, M.A..................................491
Pallaoro, A..................................441
Pallipurath Radhakrishnan, A.N ...
.......................................................43
Palmer, M....................................101
Palmgren, R...............................406
Palsson, B...................................112
Palsson, B..................................419
Palsson, B.O..............................104
Pampel, L.W.....................375, 450
Pan, X.........................................146
Pantazes, R...............................312
Papoutsakis, E.T................101, 231
Parimal, S.............................71, 391
Parish, E.J................241, 260, 281,
...................................282, 298, 299
Park, D.M..................................395
Park, S...............................272, 284
Parker, S.......................................73
Parks, L........................................56
Passno, C..................................559
Pastor, R....................................569
Pate, R.......................................100
Patel, R.......................................449
Patel, S............................9, 11, 340
Patel, S.R...................................248
Paterson, A................................571
Pathak, M....................................86
Patil, R.A....................................495
Patil, U........................................346
Paul, L.......................293, 301, 430
Paul, R........................................570
Paul, S.......................................405
Payne, G.F.................................485
Pease, M......................................15
Peck, M.........81, 42, 476, 514, 545
Peeck, L......................................129
Peeples, T.L...............................522
Peppas, N..................................222
Peralta Yahya, P..........................25
Perez-Bakovic, G.R..................186
Perez-Pinera, P.........................202
Perrin, S.B.................................569
Pertusi, D.....................................66
Peter, L......................................359
Peterson, E................................556
Pett-ridge, J................................108
Pfleger, B.....................92, 160, 211
Pham, V.....................................267
Phillips, M.W......................131, 245
Phu, M................................144, 215
Pieracci, J.P...........................7, 169
Pike, A........................................474
Pines, G.....................................210
Pirie, C.......................................416
Pirrung, S...................................386
Pitto-Barry, A.............................439
Pizzelli, K.M...............................517
Pla, I..............................................41
Podgornik, A..............................155
Politz, M.C..................................211
Pollack, N....................................124
Poosarla, V...................................67
Porterfield, W.B.........................491
Potta, T.......................................532
Prajapati, S.................................193
Prazeres, D.M...........................572
Preciado, J................................530
Prescher, J.A.............................491
Price, J.L...................................435
Price, J.V......................................97
Priluck, A....................................187
Przybycien, T.M........................428
Pu, Y...........................................150
Punreddy, S.................................15
Pushkarev, E..............................319
Q
Qian, N......................................540
Qian, X........................84, 371, 560
Qiao, J........................................238
Qin, Q.........................................536
Qu, L..........................................449
Quinn, A......................................197
Quinn, C.F..................................237
R
Rabia, L.............................140, 297
Racicot, C....................................36
Radhakrishnan, D............321, 548
Radoniqi, F................................350
Raeeszadeh Sarmazdeh, M.....31,
......................................................56
Ragauskas, A............................150
Raja, B........................................571
Rajan, R.....................................227
Rajasekaran, R..........................300
Raman, B.
Raman, N...................................362
Rameez, S.....................................2
88
Ramesh, B...................................93
Ramon, E...................................484
Ramseier, T................................124
Ramsubramaniam, N................199
Ranjan, S...................................348
Ranji, A......................................439
Rank, D......................................245
Rapp, A.......................................131
Rasala, B...................................271
Rasmussen, J.K.................83, 130
Raston, C.L...............................469
Rathbun, C.M............................491
Rathore, A.S.......................86, 132
Ravi, A........................................271
Ray, J.........................................476
Ray, J.........................................545
Rayfield, W..................................82
Razinkov, V................................227
Read, D......................................382
Reed, B.......................................126
Reed, D.W.................................395
Reed, J.......................................105
Reed, L.......................................573
Rege, K.......................34, 273, 532
Rehmann, M..............................234
Reifel, P.......................................124
Remsing, R.C............................409
Ren, B.......................309, 310, 369
Ren, C........................................227
Reyes, D....................................378
Ribeiro, L....................................199
Rice, J..........................................90
Rice, K.......................................295
Richey, C.W.......................194, 324
Rieble, S....................................430
Rieth, M.D..................................159
Rigby, S........................................15
Rios, S.......................................340
Rite, M..........................................32
Roach, M....................................124
Robbins, D.........................182, 348
Roberts, B.A..............................553
Roberts, C.J.............221, 223, 563
Roberts, R..................................124
Roberts, T..................................324
Robinson, A.S...........64, 178, 321,
...........................................521, 548
Robinson, J..............291, 396, 529
Robinson, M...............................139
Rodrigo, G.........................406, 568
Rodriguez, G.M..................96, 266
Rodriguez, R.L..........................553
Rodríguez López, A..................362
Rogers, W..................................377
89
Rogler, K..............................47, 394
Rohani, M....................................171
Romero-Hermoso, L................378
Rook, M........................................15
Rosenberg, E.............................158
Rosenman, D.J............................69
Ross, N.L...................................577
Rothstein, S..................................61
Rouse, J.C....................................72
Roush, D.J............70, 82, 240, 329
Roy, V..............................................4
Rozembersky, J.............................7
Rüdt, M.......................................133
Rueger, S...................................375
Ruppel, S...................................333
Russell, A.J................................437
Russell, K...................................228
S
Sachi, H......................................370
Sadavarte, R................................48
Sagar, A.....................................397
Saha, A........................................89
Saini, D.......................................501
Sajib, M........................................57
Saksule, A....................................12
Salm, J.........................................46
Saluja, A.....................................221
Salvachua, D.............................290
Sanaie, N...................................558
Sanchez, A................................499
Sanktjohanser, M.......................274
Saski, C........................................75
Sastry, A.....................................112
Savage, D....................................26
Savary, L......................................15
Savoy, I......................................375
Schaefer, E.J...............................79
Schaffer, D.V..............................119
Schatzer, T.................................124
Schenkelberg, C.......................457
Schermeyer, M.................220, 356
Schindler, T.................................121
Schmelzer, A..................................4
Schmidt, E.W............................507
Schmidt, M.T..............................496
Schnitzler, A.................................15
Schoborg, J................................123
Schofield, D...............................357
Schofield, M.........................47, 394
Schuetz, J..................................562
Schultz, J.S................................180
Schultz, V.L................................557
Schut, G.J..................................161
Schutsky, E..............212, 342, 387,
............................................476, 564
Schwab, M.................................356
Schwartz, A.H...................325, 561
Schwartz, C.M...........................164
Schwartz, D.K.............................52
Schwendeman, A.....................235
Seamans, T.......................247, 250
Seckute, J.................................569
Seeman, D.................................513
Sejergaard, L.............................345
Sellberg, A.........................134, 157
Sen, S.........................................410
Seo, J........................256, 308, 313
Seo, S........................................284
Seppala, S..................................159
Seppala, S....................................32
Servoss, S.L...............54, 186, 573
Sexton, J.A..................................59
Sgolastra, F.................................83
Shabbir-Hussain, M...96, 164, 360
Shah, D.A..................................495
Shah, N...........................................1
Shah, R......................................252
Shamlou, P................................350
Shamlou, P.................................414
Shang, A....................................214
Shao, Z...............................165, 470
Sharma, C..................................225
Sharnez, R.................................473
Sharpe, R...................................513
Shastry, D..................................490
Shearer, M..................................341
Shearer, R..................................173
Shearing, P.R............................359
Shen, H..............................130, 404
Shifrin, M...................................338
Shiho, H.....................................326
Shiminsky, J.................................41
Shin, H.......................................256
Shin, W......................................284
Shiratori, M................................307
Shirke, A....................................315
Shopera, T.................................445
Shukla, A.A....................................2
Shultz, J.....................................450
Shupe, A...................................545
Shusta, E...........................230, 407
Shyu, H......................241, 276, 299
Sikes, H........................................19
Simmons-Rawls, B...................164
Simons, M..................................425
Sinclair, W..................................152
Sing, K..............................514, 545
Singer, S.....................................103
Singh, N.....................................342
Singh, N.....................................387
Singh, N........................................42
Singh, N......................................476
Singh, N......................................514
Singh, N........................................81
Singh, S.....................................462
Singh, S.K....................................72
Sinkoe, A.L..................................62
Siu, K............................................27
Siwak, M.....................85, 326, 363
Skidmore, K................................173
Skoglar, H..................................343
Slade, P......................................543
Slininger, M.................................113
Sloane, S...................................352
Slocum, A...................................218
Slowinska, K..............................354
Smajic, N...................................569
Smales, M..................................322
Smirnov, D.V.................................74
Smith, H.....................................290
Smith, K.A.................................382
Smith, M.....................................438
Smith, P.R..................................474
Smolke, C.D...............................201
Snyder, M.A.................................10
Söderman, T..............................331
Soderquist, R.G........................473
Soh, K.........................................196
Soh, T..........................................441
Solernou, A................................382
Solomon, K...........32, 59, 102, 159
Somasundaram, S....................267
Song, W.....................................275
Song, Y..............................212, 564
Sorci, M.....................................569
Spatafore, D................................39
Speakman, P.............................124
Sridharan, R..............................238
Srinivasan, K................................71
Sriram, G............................197, 425
Srivastava, R..............................199
Staby, A......................................134
Stachowiak, J............................576
Stainbrook, S...............................24
Steele, A....................................332
Stein, A...............................129, 515
Steinberg, B...............................135
Steinhardt, R.C..........................491
Stepanova, N...............................74
Stephens, E.................................72
Sterl, K.......................................218
Stevens, M...................................21
Stewart, B....................................63
Stewart, J.D..............................434
Stimple, S.D......................122, 137
Stone, E.......................................89
Stone, S.....................................189
Stone, T......................................324
Stout, J.G...................................214
Stowers, C..................................124
Stratis-Cullum, D.......................447
Straub, C.T..................................161
Strillinger, E.................................117
Stroehlein, G..............................326
Strych, U....................................346
Stucky, G.D................................441
Stueckler, F................................156
Suarez-Gonzalez, B.N.............303
Suastegui, M..............................470
Subramanian, S...........................79
Sudrik, C....................................316
Sule, S.V.....................................180
Sullivan, M.O.............................577
Sumerlin, B.S............................434
Sun, J............................................51
Sun, Q.......................114, 493, 512
Sun, X.........................................371
Sunasara, K........................14, 497
Swanson, R..............................212
Swanson, R.K...........................342
Swartz, A...................................512
Sylvia, R....................................335
Szita, N........................................43
Szkodny, A.C.............................243
Szleifer, I....................................429
T
Ta, A...........................................460
Tadi, S..........................................89
Tan, Z.........................................476
Tang, L.......................................389
Tang, Y.......................................510
Tang, Y.J......................................98
Tao, L..........................................476
Tao, R.........................................460
Tao, Y..........................................127
Taralp, A.....................................381
Tarasova, Y................................468
Tashiro, Y...................................366
Tauzin, L.J.........................130, 404
Te, R...........................................560
Techner, J...................................123
Terfloth, G.J......................330, 374
Tervo, C.....................................105
TerWee, J..........................195, 236
Tessier, P.M......140, 180, 297, 565
Tessonnier, J..............................470
Thamm, A...................................106
Thangaraj, B...............................167
Tharmalingam, T........................541
Theisen, M.................................465
Theodorou, M.K..................59, 102
Thiyagarajan, B.........................306
Thodey, K...................................201
Thomas, K..................................153
Thompson, C.............................498
Thornton, C................................457
Thurber, G.M......................187, 481
Tian, J.........................................540
Tidor, B.......................................468
Tiller, K.E............................140, 297
Tilson, S.G.................................232
Timmick, S.......311, 333, 334, 392
Tingen, S....................................251
Tirrell, D.A..................................189
Titchener-Hooker, N.J..............431
Tiwari, A........................................72
Tiziani, S.......................................89
Todd, R.........................................85
Todd, R.J....................................291
Tolbert, T.J.................................524
Tomar, D.......................................72
Torres, F.....................................328
Torres, O.B................................295
Tostanoski, L.H.........................533
Trabuco, J..................................572
Tran, M.......................................271
Tran-Gyamfi, M.........................100
Traub, E......................................124
Traylor, S............................212, 361
Tremml, G..................................293
Trenchard, I.J.............................201
Triplett, K......................................89
Troccoli, N.M..............................495
Truskett, T.........................226, 555
Tsao, D.......................................238
Tucker, A....................................228
Tucker, B...................................434
Tucker, J.....................................479
Tugcu, N........................70, 84, 340
Tummala, S................................551
Tuntithavornwat, S......................22
Tyagi, D.......................................124
Tyo, K.E........................24, 66, 106
Tzanov, T....................................484
90
U
U. Nair, N......................................30
Ueda, H......................................422
Ullah, E........................................110
Umeda, A..................................358
Undey, C...........................452, 500
Urig, S........................................450
V
Vail, A...........................................83
Vaish, A........................................69
Valente, J...................................301
Valentic, A..................................562
Valverde, M................................523
van de Sandt, E................323, 386
van der Wielen, L.A..................386
Van Deventer, J.A.....................483
Vanam, R...................................315
Vandien, S.................................196
Varadarajan, N............................93
Varma, S.B................................265
Varner, J.....................................109
Vasic, J.......................................406
Vecchiarello, N.........311, 334, 392
Veeravalli, K...............................121
Velayudhan, A.K.......154, 327, 351
Verhaert, P.D.............................386
Verma, A.......................................15
Vermeer, L.................................303
Vernacchio, V.R..........................62
Vetter, T.A..................................495
Victor, H.......................................77
Vigil, A.......................................565
Vijayasankaran, N....................265
Vinci, V........................................194
Virgile, C.....................................422
Vitina, A.............................347, 539
Vlahopoulou, J...........................357
Voloshin, A...................................81
von Hagen, J.............................552
Vu, D...........................................502
W
Wagner, E..................................179
Wallby, E............................331, 343
Walther, J...............................1, 495
Wang, A.....................................185
Wang, H........................................51
Wang, J...........................................1
Wang, J......................................188
Wang, J........................................37
91
Wang, K.....................................485
Wang, M.....................................301
Wang, N.....................................184
Wang, Q.........................................8
Wang, S.....................................350
Wang, S......................................412
Wang, S......................................479
Wang, W.....................................147
Wang, W....................................404
Wang, X.....................................498
Wang, Y......................................147
Wang, Y.....................................379
Wang, Y.....................................380
Wang, Y.....................................540
Ward, B......................................124
Ward, E........................................40
Ware, V.......................................280
Warikoo, V..................................495
Warshel, A.................................261
Washabaugh, M.......................536
Watts, S........................................78
Wayman, J.................................109
Weaver, J...........................353, 371
Webster, T..................................551
Weeks, J.D................................409
Wei, T............................................20
Wei, T..........................................241
Wei, T.........................................260
Wei, T.........................................262
Wei, T.........................................276
Wei, T..........................................281
Wei, T..........................................282
Wei, T..........................................298
Wei, T..........................................299
Wei, T............................................57
Weinberg, J................................428
Weiss, G.A........................461, 469
Weiss, J......................................501
Weldon, C.L...............................180
Wellborn, W..................................14
Welsh, J.....................................388
Wen, E.......................................328
Wenger, M.................................412
Wensing, S................................124
Westerberg, K...........................433
Westoby, M.......................251, 477
Wetterhall, M....................343, 568
Weuster-Botz, D.........................117
Wheaton, G................................118
Wheeldon, I.R...........116, 164, 466
Whitaker, W.B.............................101
White, J......................................302
Whitehead, T..............................176
Whitehead, T...............................28
Whynot, A..................................252
Wickramasinghe, R...84, 371, 560
Wiley, T.H...................................243
Wilhelm, E.S..............................501
Williams, A................................294
Williams, A.J.............................219
Williams, C................................373
Williamson, S..............................56
Willson, R.C.............346, 571, 572
Wilson, N...................................266
Winzeler, G................................194
Wishner, J.................................354
Wittekind, M..............................565
Wittkopp, F.................................217
Woldeyes, M.A.........................223
Woldring, D.R............................177
Wollacott, R.B...........................513
Wong, B....................................307
Woo, J................................395, 558
Wood, D.W.........45, 122, 137, 304
Wood, T.K....................................67
Wöll, A.K...................................220
Woppmann, B............................193
Work, K......................................124
Wright, R...................................218
Wu, B..........................................100
Wu, F....................................51, 487
Wu, J...................................180, 281
Wu, S..........................................467
Wu, T..........................................465
Wu, W.........................................100
Wu, X..........................................316
Wu, Y...........................................165
Wu, Y...........................................181
Wu, Y..........................................367
Wuu, J..................................35, 307
X
Ximenes, E........................148, 153
Xu, J...........................................540
Xu, S..................................247, 250
Xu, X.........................361, 455, 540
Y
Yadav, S......................................69
Yagonia, C.F..............................567
Yaguchi, A.................................266
Yamada, M.................................121
Yamamoto, S..............................155
Yan, W..........................................89
Yang, B................................55, 296
Yang, F...............................309, 310
Yang, J........................................373
Yang, M......................................369
Yang, T........................................196
Yang, W........................................40
Yang, W.C..................................542
Yang, Y.......................................229
Yang, Y.......................................308
Yang, Y.......................................368
Yao, G........................................248
Ye, Z...........................................508
Yee, J.................................248, 545
Yeh, P..........................................227
Yi, H............................................393
Yigzaw, Y....................................373
Yik, E...........................................319
Yoo, C.G.....................................150
Yoo, J.........................................415
Yoo, S............................................3
Yoo, Y.J.....................................567
Yordy, L.....................................252
Yorita, A............................258, 444
Yoshimoto, N.............................155
You, C.........................................115
You, L.........................................540
Youchak, B................................475
Young, J............................140, 297
Yousaf, M.N.................................50
Yousofshahi, M...........................110
Yu, D.................212, 342, 476, 564
Yuan, Z.......................................184
Yun, H........................................296
Yung, M......................................395
Zheng, J....................................309
Zheng, J.....................................310
Zheng, J.....................................369
Zhou, Y.......................................357
Zhu, J..........................................116
Zhu, M........................................182
Zhu, M........................................348
Zhu, M........................................550
Zhu, Q.Q.....................................163
Zhu, S.........................................388
Zloh, M.......................................224
Zudock, E.....................................17
Zupke, C.....................................543
Zydney, A.L...................13, 16, 426
Z
Zakharyuta, A...........................381
Zarraga, I.....................................69
Zeldes, B.M................................161
Zeng, H......................................296
Zhang, H.............................73, 350
Zhang, K...............................2, 457
Zhang, M.................309, 310, 369
Zhang, N...........................361, 476
Zhang, R...................................536
Zhang, T......................................20
Zhang, W..................................504
Zhang, X...................................105
Zhang, Y....................................115
Zhang, Y....................................301
Zhang, Y...................................448
Zhang, Y......................................89
Zhao, H......................................205
Zhao, J.......................................483
Zhao, X.......................................341
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Convention Center
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Upstream Processes

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