nfast funded research projects 2012-2015
Transcription
nfast funded research projects 2012-2015
NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS JUNE 2015 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 1 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS JUNE 2015 Editors: B. S. J. Msangi, PhD Ms. Hilda Lyatuu Dr. Joseph Maziku Ms. Esther Rugakingira Ms. Angela Kabuhaya NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS i PREFACE................................................................................................................xiii Acknowledgements.................................................................................................xiv ABSTRACTS.............................................................................................................1 SOKOINE UNIVERSITY OF AGRICULTURE, MOROGORO, TANZANIA (SUA)..............................................................................2 Mwinama, G. (2013) Development of power tiller operated rice combine harvester for smallholder farmers in Tanzania............................................2 Massawe, P. I. MSc. (2012) Reponse of rice (oryza sativa l) to different phosphorus fertilizers in Lekitatu village, Meru District, Tanzania..............................................................................................2 Mirondo, R. A. MSc. (2012) Assessment of quality of mango juice and banana wine produced by small and medium scale enterprises in Dar es Salaam...........................................................3 Rukambile, E. J. MSc. (2012) Molecular characterization of theileria parva from indigenous cattle in eastern zone of Tanzania................................................................................................................3 Loina, R. F. MSc. (2013) Evaluation of the Cooperative programme for small scale dairy farmers in Muheza District.....................................4 Bivugile, D. H. MSc. (2013) Study of heirloom rice varieties and farmers perception of performance and improvement in Morogoro, Region......................................................................................................5 Aloyce, A. (2013) Detection of pathological isolates of stem rust from selected wheat fields and varieties’ reactions in Tanzania................................5 ii NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Joseph, L. MSc. (2013) Comparative of evaluation of green shanked indigenous chicken for production and egg quality traits............................6 Masao, D. F. MSc. (2013) Phenotypic characterization of the Taturu Cattle..............................................................................................................6 Robin, L. MSc. (2013) Molecular diverity of theileria parva in Kilosa District, Tanzania............................................................................................7 Chiligane, E. A. MSc. (2013) Nitrogen release from decomposed residues of herbaceous legumes and their effect on maize growth and yield in low nitrogen soil......................................................................................8 Fabian, D. MSc. (2013) Aggregate acreage response of cashew nut and sesame to commodity price and non price factors in Southeastern Tanzania..............................................................................................8 Ruboya, A. A. MSc. (2013) Effects of rotational woodlots on households’ livelihood in Meatu District, Tanzania...............................................9 Mlay, J. A. MSc. (2013) Screening buffelgrass (cenchrus ciliaris) from selected pasture seed farms in Tanzania for seed- borne microorganisms: pathogenicity and effect on germination......................................10 Musa, H. A. MSc. (2013) Evaluation of Sweet Potato genotypes for drought tolerance in the Central Zone of Tanzania............................................10 Bombo, F.B. MSc. (2013) Transaction costs in production and marketing of sugarcane under out growers’ schemes in Morogoro region of Tanzania................................................................................... 11 Sanga, D. L. MSc. (2013) Evaluation of oil fertility status and optimization of its management in s esame (sesamum indicum l.) growing areas of Dodoma District........................................................................... 11 Kalaye J. K. MSc. (2014) Yield and quality of sweet potato as affected by number of nodes buried and potash fertilizers......................................12 Wilson, C. MSc. (2014) Molecular characterization of the major histocompatibility complex DRB3.2 locus in indigenous zebu cattle in selected areas of Tanzania................................................................13 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS iii Kwigizile, O.H. MSc. (2014) Adaptability of selected snap genotypes and RESISTANCE to bean rust (Uromyces appendiculatus (Per.: Pers.) Unger var. appendiculatus) in Southern highlands zone of Tanzania..................................................................13 Kaswahili, C. R. MSc. (2014) Development of single cross maize hybrids on earliness and yield for lowland areas of Eastern Tanzania....................14 Suvi, W. T. MSc. (2014) Grain yield and quality traits of rice yellow mottle virus resistant rice (oryza sativa l.) genotypes in Morogoro, Tanzania.................................................................................................15 Nkalange, R. H. MSc. (2014) Potential and limitations of contract farming for tea out growers of East Usambara: The case of Amani division, Muheza District...........................................................15 Hiza, S. J. MSc. (2014) Livelihood implications of agricultural land loss through urban expansion and population growth in Kibaigwa area in Dodoma, Tanzania.......................................................16 Maganga, R. M. MSc. (2014) Population structure of pyricularia grisea and reaction of rice cultivars in the Southern highlands of Tanzania..............................................................................16 Rwehumbiza, J. M. MSc. (2014) Evaluation of raw cow milk quality Tanzania production in Bagamoyo and Kisarawe districtris, Tanzania..................................................................................................17 Chasama, G. L. MSc. (2013) phenotypic characterisation of Ukerewe and Bunda Tanganyika shorthorn zebu cattle in the Lake Victoria basin..................................................................................................18 Mohamed, A. MSc. (2013) Developmental biology of fruit fly (diptera: tephritidae) species attacking cucurbits....................................................18 Kalloka, M. S. MSc. (2013) Phenotypic characterization of indigenous chicken ecotypes in southern Tanzania............................................19 Kadege, E.L. MSc. (2013) Prevalence and control of seedborne iv NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS fungal pathogens of wheat in farmers saved seeds of selected locations in northern Tanzania.................................................................................20 Vicent, E. MSc. (2013) Evaluation of genotype x environment interaction on yield components of tomato (solanum lycopersicum l.) breeding lines and participatory selection......................20 Furahisha, E. H. MSc. (2013) Farmers adoption of selected recommended rice production practices: A case of Kilombero District of Morogoro Region, Tanzania....................................................................21 Kindimba, G. V. MSc. (2013) Effect of benzlyaminopurine on in vivo multiplication and genetic stability of plantain (musa spp. AAB) cv. ‘itoke sege’.............................................................................22 Makuru. H. D. MSc. (2013) Heritability, stability parameters and paths of influence on yield components of sesame (sesamum indicum l.) Breeding lines......................................................................22 Hashim, I. MSc. (2013) Banana xanthomonas wilt: incidence, transmission, pathogen characterization and management options in Kagera, Mwanza and Mara Regions.......................................................23 Ismail, M. MSc. (2013) Morphological and molecular characterization of finger millet genotypes collection in Tanzania...........................23 Joachim, J. S. MSc. (2013) Genotype x environment interaction in cowpea (Vigna unguiculata-l. Walp.) for yield performance in the Lake Zone of Tanzania............................................................................................24 Kalimba, H. F. MSc. (2013) Effect of herbicides and soil moisture on weed control, yield and quality of sugar cane (Saccharum officinarumL) at Kilombero, Tanzania............................................................................................25 Kapeleka, J. A. MSc. (2013) Effectiveness of guidelines on pesticide labels as a communication tool for smallholder farmers on pesticides handling in Northern Tanzania...............................................................................................26 Kilambo, D. L. PhD (2013) Responses of compact coffee NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS v (Coffea arabica) clones to coffee berry disease and leaf rust under varied environmental conditions in Tanzania......................................................................26 Kissimbo, M. M. MSc. (2013) An assessment of public-private-partinerships in provision of agricultural extension services in Mtwara.........................................28 Ndomba, M. D. MSc. (2013) Evaluation of soil fertility status and response of maize to different nutrients in selected soils of Tabora District......................................................................................................................28 Mbegu, S. J. MSc. (2014) Developing top cross maize (Zea mays L.) hybrids tolerant against maize streak virus (msv) disease for the Eastern Zone Ecologies........................................................................................................29 Shemahonge, M. I. MSc. (2013) Improving upland rice (Oryza sativa L.) PERFOMANCE through enhanced soil fertility and water conservation methods at Ukiriguru Mwanza, Tanzania.................................................................29 Ndee, A. K. MSc. (2013) Introgression of bean anthracnose resistance genes in common bean (PHASEOLUS VULGARIS L.) lines with ALS, CBB and BCMV/BCMNV diseases resistances......................................................30 Mohammed, R. A. MSc. (2013); Agroforestry adoption and its socio-economic contribution to livelihoods in Magu District, Mwanza, Tanzania..................................................................................................................31 Titi, U. F. MSc. (2013) Analysis of demand for private fodder delivery services for enhancing feed resource availability in Meru and Moshi rural Districts............................................................................................................31 Raphael, T. M. MSc. (2014) Effects of spacing regimes on growth, yield and wood properties of tectona grandis at Longuza Forest Plantation, Tanzania................................................................................................32 Omar, M. M. MSc. (2013) Developing rainfall erosivity equations for use in Tanzania with limited rainfall intensity data..............................................32 Mrema, E.J. MSc. (2012) Evaluation of the growth and yield performance of Watermelon varieties during the dry and wet seasons in Morogoro...............................................................................................33 vi NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Joseph, M.C. MSc. (2013) Influence of climate variability on wetland cultivation: A case of Lake Victoria shoreline wetlands in Misungwi District......................................................................................................34 Mlay, J. D. M. MSc. (2013) The potential of immunohistochemistry in diagnosis of contagious bovine pleuropneumonia in apparently health animals....................................................................................................................34 Mohammed, S. O. MSc. (2013) Estimates of stability parameters and interrelationship among yield components of orange-fleshed sweetpotato (ipomoea batatas) clones in Zanzibar.....................................................................35 Mbapila, S. J. MSc. (2013) Assessment of pigeon pea export oriented market in Babati and Karatu districts.......................................................................36 Shaali, M. S. MSc (2013) Genetic variability, heritability and interrelationships among yield components of rice (Oryza sativa L.) exotic genotypes in Zanzibar...................................................................................36 Mzula, A. MSc. (2013) Molecular epidemiology of Fowlpox virus in Tanzania..................................................................................................................37 Kihwele, L. M. MSc. (2013) The feasibility of scaling up rural institutions for sustainable land management: A case of Pemba Island..................37 Lukendakenda, P. P. MSc. (2013) Factors influencing sustainability of diary farmer groups in the Muheza District of Tanga Region, Tanzania..............38 Nzilano, B. L. MSc. (2013) Contribution of Agroforestry homegardens to household food security and income generation among communities in Mbeya Rural District, Tanzania............................................................................39 Saidia, P. S. MSc. (2013) Response of upland rice to activated Effective Microorganisms (EMA), farmyard manure and nitrogen at Ukiriguru Mwanza, Tanzania...................................................................................39 Kachiwile, A. Z. MSC. (2014) Drought tolerance assessment of cassava genotypes in a semi-arid environment in Central Tanzania.......................40 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS vii Tewele, C. P. MSc. (2012) Susceptibility to Sitophilus zeamains and nutrient content of traditionally stored maize: a case study of Mikese (Morogoro Rural District).........................................................................................40 Ngalla, R. J. MSc. (2013) Factors contributing to food insecurity during crop growing season in Handeni District, Tanga Region..............................41 Mpore, F.J.MSc. (2013) Nutrient content, Microbiological quality and sensory acceptability of sun and solar dried raisins from Dodoma Region......42 Salehe, F. S. PhD. (2014) Irrigated Agriculture and Schistosomiasis in Tanzania: A case of Morogoro and Kilimanjaro Regions.....................................42 Jeckoniah, J.N. PhD. (2013) Value chain development and women empowerment in Tanzania: a case of onion value chain in Northern Tanzania..................................................................................................................43 MUHIMBILI UNIVERSITY OF HEALTH AND ALLIED SCIENCE (MUHAS)................................................................................................44 Rweyemamu, I. K. MSc. (2013) Knowledge, Sexual behaviors, Pregnancy experiences and HIV prevention among adolescents living with HIV from early childhood in Dar es Salaam, Tanzania............................44 Lusekelo, M. MSc. (2013) Assessment of cardiovascular risk factors and level of malnutrition among the elderly of rural and urban areas in Morogoro, Tanzania...................................................................................45 Philiph, J. MSc. (2012) Bullying and associated psychosocial factors among secondary school adolescents in Dodoma municipality..................46 Kenyunko, K. M. MSc. (2013) Profile of trochanteric fractures at MOI: treatment and short-term outcome 2012........................................................46 Lucas, H. MSc. (2013) Prevalence and pattern of cardiac disorders among pregnant women attending antenatal clinic at their first clinic visit at Muhimbili National Hospital.................................................................47 viii NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS George, U. MSc. (2013) Components of systemic inflammatory response as predictors of mortality in children presenting with fever at the emergency medical department, Muhimbili National Hospital.......................48 Charles, L. L.MSc. (2013) Factors influencing sustained transmission of lymphatic filariasis in school children after eight rounds of mass drug administration: A case of mafuriko village in north-eastern Tanzania..............................................49 Longopa, G. MSc. (2013) Pattern of headache among patients attending at Muhimbili National Hospital neurology clinic-2012..............................................50 Hillary, E. MSc. (2013) Assessment of knowledge and skills of cardiopulmonary resuscitation among nurses working in critical care units at Muhimbili National Hospital, Dar es Salaam Tanzania............................................................51 Msolla, R. MSc. (2013) Factors associated with late reporting for health care among patients having oral maxillofacial tumours or tumour-line lesions attending Muhimbili National Hospital......................................52 Abdala, R. M. MSc. (2013) The experiences of informal care givers in caring for patients with cancer at Ocean Road Cancer Institute, Dar es Salaam, Tanzania........................................................................................53 Mageta, D. J. MSc. (2013) Assessment of knowledge, attitude and practice of healthy timing and spacing of pregnancy among women attending antenatal clinic at Muhimbili National Hospital, Dar es Salaam...............54 Chipeta, P. MSc. (2013) Prevalence of cardiovascular autonomic neuropathy in diabetic patients attending Muhimbili National Hospital DIABETIC CLINIC...................................................................................................55 Kalinga, L. M. MSc. (2012) Distribution of anti malaria medicines in public health facilities in Iringa Region....................................................................56 ARDHI UNIVERSITY (ARU)...................................................................................57 Siwa E. N. ( 2013); Assessing impacts of climate changes for improved beef cattle production using remote sensing and GIS technologies: A case of Ilemela and Magu Districts in Mwanza Region – Lake Victoria Basin, Tanzania..........................................................................................57 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS ix Hatibu, H. M. MSc. (2012) Investigation on production of sandcrete blocks in Dar es Salaam..........................................................................................58 Twimanye, J. M. MSc. (2013) Assessment of the contribution of housing co-operatives in the provision of affordable housing: The case of Muungano Housing Co operative Society- Chamazi...............................................58 UNIVERSITY OF DAR ES SALAAM.......................................................................59 Saria, Anasia MSc. (2013) Impact of biofuels investment on livelihoods of the rural community in Kisarawe District.............................................................59 Rwegoshora, S. A. MSc. (2012) Applicability of performance measurement during product development in manufacturing industries in Tanzania..................................................................................................................60 Tunga, M. MSc. (2014) Improvement of information flow in service delivery to teaching personnel: A case of Teachers Service Department in Tanzania..............................................................................................................60 Mbwilo, B. M. MSc. (2013) Development of online sustainable energy experiments using emona helex on ilab shared architercture with switching capability..................................................................................................61 Ngajilo, F. MSc. (2013) Rural-Urban migrants’ contribution to the livelihoods of rural communities in Makete District, Tanzania.................................61 Jabir, J. A. MSc. (2013) Cooperation between ministrial information offices and the mass media in disseminating information on public goods.............62 Kira, E. S. PhD. (2014) Integrating of meteorology and environmental education in the certificate of secondary education geography syllabus in Tanzania..............................................................................................................63 Mahongo, S. B. PhD (2014) Analysis of met-ocean processes in relation to changes in large scale climatic systems in Tanzania and the Western Indian Ocean.............................................................................................64 Shoko, A. P. PhD. (2013) Aquaculture of Nile Tilapia (oreochromis niloticus linnaeus, 1758) with an assessment of its economic benefits in the Lake Victoria basin, Tanzania..........................................................64 x NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS THE NELSON MANDELA AFRICAN INSTITUTION OF SCIENCE AND TECHNOLOGY (NMAIST)......................................................................................65 Ramadhani, A. MSc. (2013) Mobile SMS based framework toward enhancing veterinary extension service in rural areas of Tanzania.........................65 Nyamtiga, B. W. MSc. (2013) Enhanced security controls for mobile banking systems in Tanzania...................................................................................66 Julius, B. MSc. (2013) Mobile phone based information system for maternal health care in Tanzania.............................................................................66 Chambo, F. F. MSc. (2013) A mobile learning model for the enhancement of learning science subjects in secondary school education: Case study of Kilimanjaro Region...........................................................................67 Mariki, M. MSc. (2013) Towards enhancement of smallholder poultry enterprise’s performance through mobile based information management system; Case of.................................................................................67 Madila, S. MSc. (2013) Developing a prototype of e-commerce system which uses mobile-money payment system for small and medium enterprises...............................................................................................................68 Massawe, E. A. MSc. (2013) Development of a sensor system to detect adulteration of petroleum products...............................................................68 Mazima, J. MSc. (2013) A controlled biomedical signal transmission through wireless network.........................................................................................69 Kilavo, H. MSc. (2013) A system for monitoring and reporting excessive passengers in public buses.....................................................................................70 Matumo, M. B. MSc. (2013) Wireless sensor network for electric distribution system power quality monitoring...........................................................70 Kalolo, S. MSc. (2013) A price based spectrum sharing scheme in wireless cellular networks......................................................................................................71 Kazema, T. MSc. (2013) Spatial and shadow fading model for rf NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS xi optimization in W-LAN.............................................................................................71 Ngeleja, R. C. MSc. (2013) Multi-objective optimization model for energy security with harmony to economic growth and environmental protection..............72 Mgina, H. MSc. (2013) Mathematical and computational modeling of predicting intrinsic growth rates of population in Tanzania......................................72 Ngoye, A. O. MSc. (2013) Prevalence of hypertension among maasai ethnic group: The role of rural to urban migration...................................................73 Ndabigaye, A. MSc. (2013) Assessing the efficacy of a vaccine against malignant catarrhal fever under field conditions......................................................74 Msairi, C. J. MSc. (2013) Allelopathic effect of parthenium hysterophorus on seed germination, seedling growth and chlorophyll content of two nativespecies (Chloris Gayana and Alysicurpus Glumaceae).........74 Nyoki, D. MSc. (2013); Effects of Rhizobium (Bradyrhizobium japonicum) Inoculation and Phosphorus Supplementation on Productivity of Cowpea [Vigna unguiculata (l.) Walp]......................................................................75 Ngatunga, D. P. MSc. (2013) Diversity of plasmodium malaria and molecular markers of sulphadoxine resistance in Muheza, Nachingwea and Muleba Districts................................................................................................76 Mwamilawa, E. MSc. (2013) Reduction of post harvest losses of tomatoes by developing tomato powder product.....................................................76 Tairo, E. V. MSc. (2013) Effects of bradyrhizobium japonicum inoculation and phosphorus supplementation on growth and nutrient uptake by soybean (glycine max (l.) merril).............................................................77 Mubito, E. P. MSc. (2013) Assessment of antimicrobial usage and sulfonamide residues in chicken eggs in Dar es Salaam........................................77 Elisante, F. MSc. (2013) Allelopathic effect of datura stramonium on the survival of grass and legume species in the conservation areas..................78 xii NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Namkeleja, H. S. MSc. (2013) Allelopathic effects of invasive plant species; argemone mexicana to growth of selected native plant species...............79 Laizer, H. MSc. (2013) Demography and ecology of lion population in Tarangire National Park, Tanzania...........................................................................79 Raymond, J. MSc. (2013) Development of high quality sesame energy bar (Simbar).................................................................................................80 Njisya, J. MSc. (2013) Isolation of antibiotic resistant bacteria from the environment...............................................................................................80 Lukindo, T. E. MSc. (2013) Cellular IMMUNE responses elicited after VACCINATION with the HYBRID-1+IC31® tuberculosis vaccine in HIV infected Tanzanian adults.................................................................................81 Nyarobi, M. J. MSc. (2013) Prevalence of rift valley fever virus in the serengeti ecosystem, Tanzania: Evidence for inter-epidemic infections..................82 Daudi, M. MSc. (2013) Physiological stress; the impact of vaccine and malignant catarrhal fever challenge in cattle...........................................................82 Ally, M. S. MSc. (2013) Mycotoxins and heavy metal contamination in complementary foods locally processed in Tanzania........................................................................83 Mrutu, B. A. MSc. (2013) Molecular characterization of maize (ZEA MAYS L) inbred lines and hybrids in southern highlands of Tanzania..............................................................................................................84 Ngomuo, M. MSc. (2013) In vitro propagation methods of banana by using shoot tip cultures............................................................................................84 Mlalila, N. MSc. (2013) Synthesis and characterization of methyltestosteroneloaded lipid nanoparticles for production of all-male tilapia cultures........................85 Rubaka, C. MSc. (2013) Analysis of phytochemical and antibacterial activity of carissa spinarum and carica papaya crude extracts...............................86 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS xiii Kavishe, S. MSc. (2013) Quality, microbial and chemical safety of banana alcoholic beverages case study: Arusha Tanzania.....................................86 Masaki, S. S. MSc. (2013) Prevalence of type 2 diabates amongst selected Maasai ethnic group: the role of rural to urban migration..........................87 Xavery, D. K. MSc. (2013) Developing a tissue culture protocol for regenerating the threatened African sandalwood (osyris lanceolata)......................88 Ismail, S. MSc. (2013) Students’ conceptual understanding of computational round-off arithmetic in Tanzanian schools: A case study of high school students in Hai and Moshi Urban Districts..................88 Kahuru, S. MSc. (2013) Mathematical model for water resource planning in irrigation: The case of wine grape farming in Dodoma Region.......................................................................................................89 Ndakidemi, C. MSc. (2013) Natural regeneration trend of the threatened brachylaena huillensis in Bombo West Forest Reserve and development of sanitation protocol for in vitro propagation..............................90 Mzena, T. P. MSc. (2013) Evaluation of nutritional values and medicinal pyhtochemicals of wild edible mushroom species growing in Mufindi District, Tanzania.......................................................................90 MZUMBE UNIVERSITY, MOROGORO, TANZANIA..............................................91 Justine, P. K. A. MSc. Financial sector’s reform in Tanzania: what are the outcomes of financial reform to economic growth..............................91 SCINTIFIC ARTICLES PUBLISHED FROM COSTECH FUNDED RESEARCH ROJECTS..........................................................................................92 R. R. Minja1, J. Ngailo2, N. Mwalukasa2 and S. Kyando2 (;2014); Effects of Phosphate Rock on Phosphorus Availability for Vegetable xiv NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Crops in Cropping Sequence..................................................................................92 Zacharia Katambara1, Frederick C. Kahimba2, Winfred B. Mbungu2, Paul Reuben2, Muyenjwa. Maugo3, Fikiri D. Mhenga2, and Henry F. Mahoo2 . Optimizing System of Rice Intensification Parameters Using Aquacrop Model for Increasing Water Productivity and Water Use Efficiency on Rice Production in Tanzania.............................................93 Zacharia Katambara1*, Frederick C. Kahimba2, Henry F. Mahoo2, Winfred B. Mbungu2, Fikiri Mhenga2, Paul Reuben2, Muyenjwa Maugo3, Anthony Nyarubamba3 . Adopting the system of rice intensification (SRI) in Tanzania:.............................................................................93 Kahimba, F.C.1, Kombe, E.E.2, and Mahoo, H.F.1. The Potential of System of Rice Intensification (SRI) to Increase Rice Productivity: a Case of Mkindo Irrigation Scheme in Morogoro Region, Tanzania......................94 P Y Kavana and V A M Kakengi* Availability of pasture for domestic and wild herbivores in grazing land of Mpanda Tanzania........................................95 1Christopher L. Materu, 2Seguni Z. Kombo and 3Linus Masumbuko. Ant species diversity and composition at Mlingano mango orchard in Tanzania.................................................................................................95 Christopher L. Materu1*; Zuberi Seguni2; Evarista, E. Shao, Beatrice M. Mruma, Andrew J. Ngereza2. Assessing the Impact of Integrated Pest Management Programme for Management of Fruit Flies (Diptera: Tephritidae) On Mango Orchards in Tanzania..................................96 Samwel Mchele Limbu ;Amon Paul Shoko ; Hieromin Amon Lamtane; Evantuce Dominick Shirima ; Mary Alphonce Kishe-Machumu; Huruma Fredrick Mgana; Yunus Daud Mgaya. Effect of initial stocking size of the predatory African sharptooth catfish (Clarias gariepinus) on recruits, growth performance, survival and yield of mixed-sex Nile tilapia (Oreochromis niloticus) in concrete tank culture system................................................................97 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS xv PREFACE The Tanzania Commission for Science and Technology (COSTECH) was established in 1986 by Act of Parliamentary No.7 as a successor to the Tanzania National Scientific Research Council (UTAFITI) with the responsibility of coordinating and promoting research and technology development activities in the country. The Commission is an apex organ with the responsibility of advising the Government on the use of Science, Technology and Innovation for socio economic development. Part V of the Act that established the Commission provides for the establishment of the National Fund for the Advancement of Science and Technology (NFAST) which is managed and administered by the Commission. The Fund is basically intended to support research with special emphasis in the national priority areas; support development and transfer of appropriate technologies; support capacity building in R&D and S&T activities in terms of human resources and research facilities; support organization of/ attendance to scientific fora and information dissemination through publications; promote innovativeness and inventiveness through the provision of awards; commission individuals, groups of individuals, institutions or groups of institutions to undertake research or studies in special areas identified by the Commission to be of a national interest and support any other activities whose objectives would be the promotion of S&T for national development. COSTECH on behalf of the Government has been providing research funds for among other things, supporting research grants and fellowship for Msc and PhD programmes in various R & D Institutions and Universities from year 2010. Since then, COSTECH through NFAST has supported 76 research grants and sponsored 517 researchers for postgraduate studies in the local Universities at the level of MSc (343) and PhD (174). This came out as one of the strategies to address the issue of limited research human resources base in R & D institutions including Universities. xvi NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS This Book of Abstracts provides a total of 144 abstracts of research findings from 8 research grants as well as 136 dissertations of NFAST funded MSc and PhD programmes. The entire community of the Commission hopes that you will find the contents of the abstracts useful and a dependable reference material for your future endeavors. The organization of this book starts with abstracts sourced from dissertations published by NFAST funded fellows then followed by publications of NFAST funded research grants results sourced from various journals as indicated in the subtitles of each abstract. COSTECH is grateful to the Chairman of the Board of Commissioners and the Government of The United Republic of Tanzania to allow it to demonstrate its potential in supporting research that in turn will provide opportunities to Tanzanians to exploit National resources more effectively and sustainably. Dr. Hassan Mshinda Director General NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS xvii Acknowledgements First and foremost, The Unit that Coordinates the National Fund for Advancement of Science and Technology (NFAST) would like to thank the contribution of the authors of the abstracts of this book for their entire commitments that has been quite encouraging. NFAST Unit on behalf of the Commission also acknowledge all Universitie’s Managements and members of staff particularly Directors of Research and Postgraduate Studies at Sokoine University of Agriculture (SUA), Muhimbili University of Health and Allied Sciences (MUHAS), University of Dar es Salaam (UDSM), Nelson Mandela African Institute of Science and Technology (NMAIST) and Ardhi University (ARU) for submission of dissertations to the Commission as agreed in the fellowship contracts. COSTECH is in addition wish to express her sincere appreciation to R and D Institutions for cooperation and supervision of the funded projects without which this publication would not be possible. I wish to express my confidence to the entire production team in the preparation of this book of Abstracts. This work would not have been effectively accomplished without the financial and material support from the Board of Commissioners in supporting the program and publishing costs of this book. Finally, the Unit is grateful to the Board of Commissioners and Government of The United Republic of Tanzania for allowing it to demonstrate its potential in supporting research that in turn will provide opportunities to Tanzanians to exploit National resources more effectively and sustainably. B. S. J. Msangi (PhD) NFAST Coordinator FUNDED RESEARCH PROJECTS 2012-2015 xviii NFAST A BOOK OF ABSTRACTS SOKOINE UNIVERSITY OF AGRICULTURE, MOROGORO, TANZANIA (SUA) Mwinama, G. (2013) Development of power tiller operated rice combine harvester for smallholder farmers in Tanzania Abstract This study was conducted to develop a Power Tiller Operated Rice Combine Harvester (PORCH) for small holder farmers in Tanzania. PORCH can be attached to a power tiller during harvesting operation and detached to give the power tiller a room for other operations. The study aimed at designing, construction and testing performance of PORCH prototype. Design specifications and drawings were developed and prototype was constructed. The performance of PORCH prototype was tested in Lower Moshi and compared to manual harvesting. Comparisons made included manpower required (man-h/acre), harvesting capacity (acre/h), grain loss (%), presence of material other than grain (MOG %) in the harvested grain, and harvesting cost. It was found that in PORCH harvesting average values of man-h, harvesting capacity, cost, grain loss and MOG were 4.76 h/acre, 0.42 acre/h, Tsh. 30151/acre, 28.86% and 3.19% respectively. While the average values of man-h, harvesting capacity, cost, grain loss and MOG for manual harvesting were 255.9 h/acre, 0.0039 acre/h, 181250/acre, 14.48% and 8.3% respectively. Hence PORCH harvesting was better for all performance measures made except grain loss reduction. More grain loss in PORCH harvesting was due to leakages in some of PORCH functional elements. Grain loss can be reduced by avoiding leakages in PORCH functional elements. It was recommended in the future that modifications should be done so as to alleviate shortcomings in PORCH functional elements. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 1 Massawe, P. I. MSc. (2012) Reponse of rice (oryza sativa l) to different phosphorus fertilizers in Lekitatu village, Meru District, Tanzania Abstract A study was conducted to evaluate the response of rice to P and P use efficiency from MPR, Minjingu mazao and TSP fertilizers under irrigated rice (Oryza sativa L.) production in Lekitatu village. The fertility status of the soils and their suitability for rice production at two experimental sites were evaluated based on technical indicators of soil fertility. The major soil fertility limitations included low soil organic matter, low total nitrogen and medium available phosphorus hence the rice soils in Lekitatu village were categorized as of low fertility status and moderately suitable for rice production. Phosphorus was applied at rates of 0, 20, 40 and 60 kg P ha-1 as MPR, Minjingu mazao and TSP. Nitrogen was applied uniformly at a rate of 60 kg N ha-1 as urea to the MPR, Minjingu mazao and TSP treatments plots taking into account the 10% N contained in the Minjingu mazao fertilizer. The P fertilizers were broadcasted and incorporated into the soils before transplanting the rice seedlings and N was applied at two equal splits, namely at tillering and panicle initiation stages. The ranges in yield components between the control (0 kg P ha-1) and the highest levels of P (60 kg P ha-1) were 12-22, 12-19 and 12-22 number of tillers per plant, 7.67-25.97, 7.67-26.83 and 7.67-30.20 tha-1 dry matter yields and 1.5-8.63, 1.59.23 and 1.5-10.43 tha -1 grain yields for MPR, TSP and Minjingu mazao, respectively. The P fertilizers applications increased rice yield components, N and P contents in the rice plants, residual soil P as well as plant PUE as the levels of P increased from 0 to 60 kg P ha-1 for all P sources. The yield components increased significantly (P<0.05) with increasing rates of P application. The increased were due to increased availability and uptake of plant nutrients particularly P. Based on the generated yields data, it was thus recommended that: Minjingu mazao at the rates of (40 t0 60 kg P ha-1), MPR and TSP at a rate of 60 kg P ha-1, respectively could be adopted for increased and sustainable rice production in Lekitatu village. 2 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mirondo, R. A. MSc. (2012) Assessment of quality of mango juice and banana wine produced by small and medium scale enterprises in Dar es Salaam Abstract This study was performed to evaluate the nutritional and microbiological quality of industrially processed and street vended mango (Mangifersa indica) juices and banbana (Musa Sapientum) wines from thirteen different processors in Dar es Salaam. Street vended juices reported high level of Ash content, pH and reducing sugars whereas industrially processed juice had higher levels of vitamins C. These parameters were significantly different at P<0.05. The titratable acidity (TA) of street vended juice samples ranged from 0.45-0.64% and for industrially processed ranged from 0.38-0.62%. For wine , the pH ranged from 4.70-5.14 with titratable acidity ranging between 0.95 and 2.23%whereas total soluble solids (TSS) ranged between 4.30% and 10.79%. Both ethanol and methanol content determined were within the range set by Tanzania Bureau of Standards. In microbiological analysis the street vender juice reported presence of faecal coliforms which ranged between 7.3 x 10 to 2.4 x 104 CFU/ml, yeast (1.21 x 104 -5.95 x 102 CFU/ml, mould (7.73 x 102 – 2.68 x 102 CFU/ml and Total Planet Count ( TPC) ( 1.317 x 103 – 1.76 x 102 CFU/ml). The fungi detected were Candida, Saccharomyces, Cladosporium, Penicillium and Aspergillus spp. industrially processed juices and wines were devoid of coliforms and moulds but Saccharomyces spp. Were detected in some samples. Based on the results obtained, street vended juices were highly contaminated posing health risks to consumers. It was therefore suggested that training should be offered to the street vended juice processors on Good Hygienic Practices (GHP) and other methods that would help to reduce the levels of microbial contamination. Some of the challenges encountered by the processors were; lack of trained personnel and market information, insufficient funds and technology, difficulties in obtaining packaging materials, poor power supply and no links with research institutions that would assist them in dealing with technical problems. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 3 Rukambile, E. J. MSc. (2012) Molecular characterization of theileria parva from indigenous cattle in eastern zone of Tanzania Abstract Theileria parva is a haemoprotozoan parasite that causes fatal lymphoproliferative disease of cattle, East Coast fever (ECF), transimitted by Rhipicephalus appendiculatus tick. ECF severely constraints the livelihoods of poor livestock-keepers in East Africa. There are many strains/stocks of T. parva differing in many characteristics. Immunity elicited by immunization is strain/stock specific and therefore, it is appropriate to characterize T.parva to find most relevant stocks for inclusion in a vaccine. Crossimmunity trials and monoclonal antibodies have been used for characterization of T.parva, but difficulties in determining the change in the characteristics of the parasite after tick- cattle passage and cross reactivity are among the drawbacks in the use of these methods. The only accurate methods for characterization of T. parva stocks thereby selecting the candidate for vaccine are molecular techniques. This study used conventional polymerase chain reaction (PCR) and semi-nested PCR to amplify antigenic genes coding for sporozoite surface (p67) and piloplasm proteins (p104) respectively using DNA extracted from cattle blood samples naturally infected with T.parva. Restriction fragments length polymorphism (RFLP) profiles of p104 were generated by Arthrobacter luteus 1 (Alu 1 ) restriction enzyme. Two types of p67 bands were identified, 750 bp and 950 bp. Profiles for p104 PCR-RFLP revealed two types of stocks;one stock with 340 bp and 500 bp bands, and another with 220 bp and 280 bp bands. These bands were identical to those of T.parva Katete and T.parva Muguga respectively. Three DNA samples showed mixed infection of either T.parva or another species of Theileria. They have 3-5 bands of sized ranging from below 100 bp to 340 bp. Therefore in this study, two types of T.parva stocks were observed. T. parva Katete, T.parva Muguga and Theileria stocks with multiple bands. With respect to p67 gene amplification, 750 and 950 bp alleles were identified. This study has found unequivocally that there are T.parva stocks in Tanzania which are not in the imported ECF trivalent vaccine (“Muguga cocktail”) currently in use. The study recommends characterization of T.parva local stocks for inclusion in the vaccine against ECF. 4 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Loina, R. F. MSc. (2013) Evaluation of the Cooperative programme for small scale dairy farmers in Muheza District Abstract This study was conducted to evaluate the cooperative breeding programme for small scale dairy farmers in Muheza District. A total of 90 (64 cooperative members, 26 non- cooperative members) farmers and 4 extension officers were interviewed using questionnaires and Focused Group Discussion (FGD) to gather primary data. The secondary data were collected from the cooperative office and farmers’ records. The district was studied covering four zones namely; Muheza East, Muheza West, Amani East and Amani West. This study revealed that the cows kept by cooperative members performed better in terms of milk production and reproduction performance as compared to farmers who are not members of the cooperative. The cooperative provides market stability of milk, heifers on credit and training services but the price of milk set by the cooperative was considered by the farmers to be low. There was poor linkage among the members, cooperative managers and other actors. Eighty two percent of the interviewed farmers reported not to have technical support from Tanga Fresh Ltd. (the sole bulk buyer) while 86.8% of the farmers had no access to knowledge sharing among members. Sixty eight percent of the farmers opted to use natural service due to non-availability, reliability or cost of Artificial Insemination services. Most 94% of the breeding bulls were of Friesian breed and the remaining 6% were Ayrshire. The dairy herd comprised of a cross between local and Friesian or Ayrshire and local. On average farmers owned 1 to 5 dairy cows producing average of 8.17= 1.27 litres of milk per cow/per day. Many farmers are keeping records but they lack enough knowledge in utilizing them. It is therefore concluded that the major problems facing farmers in Muheza District include low price of milk, lack of quality animals, unreliable bull/semen services, lack of adequate veterinary and extension services and lack of adequate dairy inputs NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 5 Bivugile, D. H. MSc. (2013) Study of heirloom rice varieties and farmers perception of performance and improvement in Morogoro, Region Abstract A study of heirloom rice varieties and analysis of farmers’ perception on their performance and improvement was conducted in 2011/2012, using survey and field experimentation. About 120 farmers from rice growing villages in Morogoro Region were interviewed using a questionnaire, during which seed sample of the heirloom varieties were collected. Field characterization trial of the collected varieties was planted accompanied with particular evaluation trials for selection of the collected varieties involving two groups of farmers, from Mlali and Turiani. Data collected were analysed using SPSS for survey data, pair wise and matrix ranking for PVS and Genstat for morphological characterization with differences of means using Tukey’s mean separation test. The survey results showed that there is a great number of farmers using local varieties despire the release of some improved varieties. Twenty one rice varieties were collected in total during the survey and in these only one was an improved variety. Outstanding characteristics of these varieties by farmers’ perception included those against disease and pest, low yield and drought. Using matrix ranking, SARO 5 was rated overall best for Turiani farmers with the best performance in yield; whereas Kihogo was the best in palatability and aroma for Mlali farmers. This shows that farmers from different locations have different perceptions. Varieties Mbawambili, Supa Mbeya and Kihogo appeared in all the ranking tools as the best overall. This also shows that there are shared important characteristics in these varieties that can be explored for general breeding purposes. Morphological characterization of the varieties showed that there was significant difference among the varieties in all parameters (P≤ 0.05). This study indicates the importance of farmer’s participation in variety selection including incorporating their perception in breeding and variety development program as one of the ways to increase adoption rate of improved varieties. 6 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Aloyce, A. (2013) Detection of pathological isolates of stem rust from selected wheat fields and varieties’ reactions in Tanzania Abstract This study was conducted to determine Status of wheat stem rust disease in Tanzania and how the available wheat varieties react to the prevailing isolates of this disease. The study aimed at identifying isolates groups and resistant varieties, and draw implications for farmers, research and extension. Wheat stem rust disease survey was conducted in twenty fields from Manyara, Mbeya, Iringa Kilimanjaro and Arusha regions. Of all the fields surveyed 60% were infected by stem rust disease with severity between 26 – 50%. Fifteen percent of fields were affected at 11 – 25% disease severity and 10% had 51 – 75%. The lowest disease severity of 5% was scored in Iringa region whilst in northern highlands none of the field was infected at a severity level of less than 15%. Seventeen isolates of Pucinia graminis f. sp. Tritici collected from the selected regions were all virulent to Viri wheat variety. Isolates from Manyara and Arusha were more virulent followed by those from Kilimanjaro, Mbeya and Iringa. The most destructive isolates encountered in different regions caused various severity on Viri wheat variety with average severity of 37.5%. Most varieties (Mamba, Mbayuwayu, Selian, Juhudi, Azimio 87, Chiriku and Riziki) under production were susceptible to wheatstem rust disease. Sifa variety response to wheat stem rust isolates both in the field and under controlled environment indicated an outstanding resistant to the most aggressive isolates and is thus recommended for disease management. Technology transfer is significantly important for formers to get rid of using susceptible varieties e.g. Viri. Plant protection strategies such as crop rotation and weeding are not implemented accordingly in some areas; such cases need to be addressed as well to improve wheat production in Tanzania. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 7 Joseph, L. MSc. (2013) Comparative of evaluation of green shanked indigenous chicken for production and egg quality traits Abstract A study was taken at Sokoine University of Agriculture to investigate the influence of shank colour on egg quality traits and production performance. The study involved green shanked Indigenous (GSI) chickens, yellow shanked Indigenous (YSI) chickens and Rhode Island Red (RIR) genetic groups. The genetic groups were reared under the same environment and management. A total of 240 blood plasma samples were used in assessment of plasma cholesterol content while 120 egg samples were used to assess egg yolk cholesterol and other egg quality traits. RIR differed significantly (P≤ .05) from GSI and YSI genetic groups in terms of egg number, egg weight, egg mass, laying intensity, yolk ratio and yolk albumin ratio. However, there was no significant difference between the indigenous genetic groups for these parameters as well as fertility, hatchability, growth rate and survival rate. The mean weights were 40.1 ≠ 0.8, 42.3 ≠ 0.9 and 52.0 ≠ 0.7 gm while the laying intensities were 46.7 ≠ 1.76, 46.8 ≠ 1.7 and 76.6 ≠ 1.7% for GSI, YSI and RIR genetic groups, respectively. There was no significant difference in egg yolk cholesterol, whilst plasma cholesterol differed significantly between RIR and indigenous types. The mean egg yolk cholesterol were 196.2 ≠ 7.2, 210.1 ≠ 7.2 and 201.8 ≠ 7.2 mg/ dl for GSI, YSI and RIR respectively, while the blood plasma cholesterol were 210.4 ≠ 7.5, 196.5 ≠ 7.5 and 152.1 ≠ 7.5 mg/dl for GSI, YSI and RIR respectively. Furthermore, the study revealed that, egg yolk cholesterol increased gradually with age while plasma cholesterol decreased with age in all genetic groups of this study. 8 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Masao, D. F. MSc. (2013) Phenotypic characterization of the Taturu Cattle Abstract A study on phenotypic characterization of the Taturu cattle was undertaken in Manyoni and Iramba districts of Singida region. Furthermore, the study aimed at carrying out household characteristics of Taturu pastoralist and husbandry practice of their cattle. A total of six villages were surveyed in each district and ten household per village were sampled for in- depth analysis. Body measurements of Taturu cattle such as body weight length, heart girth, height and withers, horn length, ear length and width were taken from a total of 116 live animals. Data on household socio-economic characteristics and cattle adaptive traits were collected through individual interviews of 120 farmers using a structured questionnaire. Adaptive attributes observed on Taturu cattle include tolerance to drought (90%), disease (98%), heat (74%), and better draft power (93%). The average mature weight of bulls and cows were 295.5 kg and 217.3 kg, respectively. Mean weights of bulls and cows in Manyoni were higher (p<0.05) than in Iramba. Taturu cattle in Manyoni had higher body hight (p<0.05) than in Iramba. Sex affect was significant (p<0.05) for all linear body measurements. Mean values for heart girth (151.2cm), withers height (111.3cm) and body length (119.7cm) for bulls were higher (p<0.05) than in cows. The coat colour varied from grey (16.3%), brown (14,3%), black (13.7%), red (10.7%), white(9.3%) or multicolour (35.67%), where by one or more colours were found in one animal. The udders were moderately well developed with rather small teats. Selection criteria for cows were for milk yield, growth rate and fertility while for bulls were dams’ milk production potential, ability to withstand hot condition, low food and water during the dry seasons. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 9 Robin, L. MSc. (2013) Molecular diverity of theileria parva in Kilosa District, Tanzania Abstract Theileria parva is an apicomplexan protozoan parasite causing East Coast Fever in Eastern and Central Africa. A study conducted between February and May 2012 in six villages in kilosa district in Tanzania to determine the diversity of the T.parva parasite circulating in the district. Also data of the disease cases reported to Veterinary investigation centres (VIC’s) and compiled by the Ministry of Livestock Development and Fisheries, were analyzed to gain insight into the current status of ECF in Tanzania. To determine the diversity, convectional Polymerase Chain Reaction (PCR) was used to amplify antigenic genes coding for sporozoite surface protein (p67) using DNA extracted from cattle blood samples naturally infected with T. parva and T.parva isolated from ticks. A total of 100 cattle blood and 95 tick samples were collected. PCR products (banding Pattern) were analyzed to characterize T.parva parasite that occur in cattle and ticks. Analysis of the PCR products suggested the existence of two groups of T.parva parasites with band sizes 800 and 900 by respectively. This study has found that there is T.parva allele in Kilosa that is not in the imported ECF trivalent vaccine (“Muguga cocktail”) currently in use. Three year records (2008-2010) of the disease indicate lower prevalence of ECF compared to prevalence reported by other previous studies. The low prevalence observed in this study suggests that reports from Vic’s cannot give good estimates of ECF prevalence due to under reporting, poor diagnosis, poor records keeping and otjher in the field. It was recommended that more research has to be conducted so as to establish the complete list of parasite circulating in Kilosa and the region. 10 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Chiligane, E. A. MSc. (2013) Nitrogen release from decomposed residues of herbaceous legumes and their effect on maize growth and yield in low nitrogen soil Abstract This study was conducted at Sokoine University of Agriculture (SUA) located at 60 51’5”S and 37 039’26”E at 525 masl at the site with sandy clay soil pH 5.16 with the overall objective of improving maize productivity through the use of leguminous residues as source of nitrogen (N). Specifically the study aimed at determining the mineralization pattern and total amount of released from decomposition of legume residues; to assess the effects of legume residues on maize growth and yield and to evaluate the effects of legumes biomass applied on maize yield. The treatments used were control (no legume residue and fertilizer application); application of N (urea); residues in form of velvets bean (mucuna prurient L.), dolichos (lablab purpureus L.) and sunhemp (crotalaria ochroleuca G.) Maize variety ‘’staha’’ was used as a test crop. Experiments conducted were laboratory mineralization experiment, screen house and field experiments. These experiments were conducted between November 2011 and June 2012. Results showed that sunhemp had the highest N content of 2.77% followed by velvet been and dolichos with 2 .49 and 2.42%, respectively. Total amount of release over 16 incubation weeks differed significantly (P< 0.05) with the range of 151.22 ug kg -1. Leguminous biomass production was 15.13, 12.67 and 11.75 t ha -1 for velvet bean, dolichos and sunhemp, respectively. Maize grain yield range from 1.02 for control to 3.96 t ha -1 with Urea application. Sunhemp velvet bean and dolichos resulted in 3.69, 3.6 and 3.49 t ha -1, respectively. As the incorporation of leguminous residues resulted into improvement of soil physical characteristics such as texture, bulk density and moisture content, It is recommended for use in mineral low N soils. This application will enhance resources farmers to improve maize productivity. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 11 Fabian, D. MSc. (2013) Aggregate acreage response of cashew nut and sesame to commodity price and non price factors in Southeastern Tanzania Abstract This study aimed at investigating the impact of price and non-price factors on cashew and sesame acreage in Nachingwea and Mtwara rural Districts. Growth rates analyses were also conducted through linearlized exponential growth model to trace the trends for area, yield (productivity) and production of the two crops for the period 1995-2010. The general trend showed positive growth rates in area, yield and production, but with few exceptions. Meanwhile, the logarithmic functional form of the linear Nerlovian adjustment model was employed on time series data from 1995-2010 to estimate acreage response to price and non-price factors. Results revealed that there was a positive and significant relationship of sesame acreage with price, and non-significant relationship with rainfall (non-price factors) in both districts. Similarly, cashew acreage was observed to have positive and significant relationship with price and positive but insignificant relationship with rainfall in Nachingwea District. In Mtwara rural, positive relationship existed between cashew acreage and price, while with rainfall the relationship was negative. The study further established that short and long run price elasticities of sesame acreage were 0.264 and 0.515, relationship in Nachingwea, whereas short and long run price elasticities in Mtwara rural were 0.478 and 1.65, respectively, which implies that farmers are more responsive to price changes in the long run than they are in the short run. Similarly, non-price short and long run elasticities for sesame were 0.035 and 0.06, respectively in Nachingwea; and 0.032 and 0.11, respectively for Mtwara rural District. For cashew acreage, the short and long run price elasticities were 0.326 and 1.364, respectively for Nachingwea while in Mtwara rural short and long run price elasticities were 0.37 and 0.885, respectively. Meanwhile the study found that short and long run non-price elasiticities for Nachingwea were 0.033 and 0.049, respectively. However, the elasiticities though positive, they generally fell in the inelastic range suggesting that price incentive in itself is essential but not sufficient. 12 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Ruboya, A. A. MSc. (2013) Effects of rotational woodlots on households’ livelihood in Meatu District, Tanzania Abstract Meatu district in Simiyu region is one of the most degraded areas in Tanzania where the effects of deforestation were vivid resulting from increased population of both humans and livestock. The problem extends to the effect of soil degradation, poor crop yield, shortage of fuel wood, building materials and fodder. Rotational Woodlot (RWL) was the most promising tested technology to solve the problem. The objective of this study was to assess the effects of rotational woodlots on households’ livelihood. The study was carried out in two wards, two villages per ward and thirty households from each study village. Both primary and secondary data were collected to address the objectives. Methods used for primary data collection included household survey, key informant interviews using a checklist and direct observation. Literature survey was done to obtain secondary data. Qualitative data was analyzed using content analysis while qualitative data was analyzed through a Statistical Package for Social Sciences (SPSS) computer soft ware version 12. Result indicated that 44.4% of total household income and 62.0% of the total household food produced was obtained from rotational woodlots. Socio-economic was: age of household head, land ownership, food produced from rotational woodlots while constrained factors were: sex and education level of the head of the household, land under RWL and household size. Socio-economic factors which enhanced food security were: sex and occupation of the head of the household, size of the household, land under RWL and income from RWL. The constraining factors were: age, education level of the head of household, the rights to land. The study concludes that, RWL has positive effect of household’s income and food security. Based on the socio-economic findings it is recommended that RWL be up scaled through establishing tree nurseries in the villages, as well as recruiting more extension staff and conducting frequent monitoring and evaluation in the villages practicing rotational woodlots. Moreover, there is need of taking into consideration socioeconomic factors that enhance and constrain RWL based on income and food security. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 13 Mlay, J. A. MSc. (2013) Screening buffelgrass (cenchrus ciliaris) from selected pasture seed farms in Tanzania for seed- borne microorganisms: pathogenicity and effect on germination Abstract Buffelgrass (Cenchrus ciliaris) is one of the important perennial grasses in the pasture industry in Tanzania. It is drought tolerant, nutritious and has rapid growth characteristics. Three hundred sixty grams of seed samples of C. ciliaris collected from six selected pasture seed farms were screened for seedborne microorganisms at the African Seed Health Centre, Sokoine University of Agriculture, Morogoro, Tanzania. The lotter method, the direct plating and the Top of Paper methods were used for fungal, bacteria and seed germination tests, respectively.Fungal and bacteria pathogens of economic importance that were detected included; Phoma spp. (28.5%). Curvularia lunata (17.34%), Alternaria alternate (14.09%) Bipolaris spp. (12.2%), Acidovorax, Xanthomonas and Pseudomonas spp. Characterization using morphological and biochemical tests including molecular techniques and pathogenicity of fungal and bacteria strains were done on buffelgrass seedlings. Results indicated that seed germination decreased (<50%) with an increase in fungal infection. Seed samples from LITI Tengeru had the lowest seed germination (8%) and high fungal (37.8%) incidence on their caryopses. Pathogenicity of Bipolaris spp. , Phoma ssp., Pyricularia grisea, Fusarium pallidoroseum, Exserohilum rostratum, Nigrospora oryzae and Acidovorax, Pseudomonus and Xanthomonas bacteria strains were confirmed on C. ciliaris seedlings. Some species of the detected fungi were found in both spikelets and caryopses. Further research is needed on losses caused by fungal infection in buffelgrass seeds. 14 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Musa, H. A. MSc. (2013) Evaluation of Sweet Potato genotypes for drought tolerance in the Central Zone of Tanzania Abstract Drought in the most significant factor restricting crop growth and productivity in most agricultural fields of the world. The purpose of this study was to identify sweet potato genotypes tolerant for drought that could be used in breeding programs for improving sweet potato production in Tanzania. A field experiment involving 17 sweet potato genotypes was conducted at the Agriculture Research Institute (ARI) Hombolo, Dodoma between April and September 2012 (dry season). The experiment was laid out in a randomized complete block design (RCBD) with three replications. The genotypes were evaluated under well watered (WWW) and water stress (WS) conditions where moisture level was maintained at field capacity until 60 days after planting (DAP). In the WW plots, supplementary irrigation was applied up to maturity at two days interval to maintain moisture level at field capacity while for WS irrigation was stopped at 60 DAP. In the WW plots, supplementary irrigation was applied up to maturity at two days interval to maintain moisture level at field capacity while for WS irrigation was stopped at 60 DAP. Data was collected on yield, morphological parameters and level of antioxidant activity (catalase and peroxidase) and analyzed by GENSTAT, 13th Edition. Under WS conditions, the highest yield observed was 2.8 t ha -1 compared to 15 t ha -1 for WW. The study revealed that WS SPKBH06/216, Simama, D12-02, SPKBH01/264 and Polista. On morphological parameters, the study revealed a reduction in plant size under WS. The level of antioxidant enzyme activities catalase and peroxidase showed to be higher under WS. Implying accumulation of reactive oxygen species. Thus such higher levels could be used as an indicator for drought tolerant genotypes in sweet potato. From farmer’s participatory evaluation for culinary characteristics it was revealed that genotypes Polista, Simama, Pipi and SPKBH06/216 were the most preferred in terms of root taste, color and dry matter. The above genotypes could be used as breeding materials. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 15 Bombo, F.B. MSc. (2013) Transaction costs in production and marketing of sugarcane under out growers’ schemes in Morogoro region of Tanzania Abstract The purpose of this study was to analyse the production and marketing transaction costs of sugarcane out growers in Mvomero and Kilosa disrtricts of Morogoro Region in Tanzania. The specific objectives were to determine the level of transaction costs in different categories of out growers, to examine the influence of institutional arrangement on sugarcane transaction costs, and to examine the impact of transaction cost on the quality of sugarcane in terms of sucrose content (Rendement). Data were collected through a cross sectional survey by using structured questionnaire, complemented by focus group discussions, personal interviews with key informants, and field observations. A total of 160 sugarcane out growers (80 from Turiani and 80 from Ruembe) were randomly selected and interviewed. Checklists were used to gather information from sugarcane participating institutions. Descriptive statistics and regression analyses were used to analyze the data. The result showed that distance from the field to the factory is the only significant factor affecting the transaction cost positively in both study area. Regression analysis was also used to investigate the effects of transaction cost on sugarcane quality. The study revealed an inverse relation between transaction costs and rendement level with insignificant variable under study and weak explanatory power of the model. The study also revealed that there are several productions and marketing problems that hinder prosperity of out growers’ involvement is sugarcane production. The existing institutional arrangement has resulted in inefficient services delivery, losses to out growers and increase transaction costs; the study recommends reorganization of the institutional set up of sugar industry; settings up policy that would create a good working environment between sugarcane out growers and the factories. 16 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Sanga, D. L. MSc. (2013) Evaluation of oil fertility status and optimization of its management in s esame (sesamum indicum l.) growing areas of Dodoma District Abstract A study was conducted in Dodoma district, Dodoma region Tanzania to assess the soil fertility status for sesame production. The study was initiated due to the continuous decline of sesame yield to alarmingly low levels. The objectives of the study were to determine the levels of N, P, K, S, Mg, Ca, Na, Zn, Cu, Fe and Mn in soils and evaluate the response of sesame crop to the application of N, P, K, S and Zn. This was done by analyzing soils from twenty sesame growing areas of Dodoma district. The analysis included determination of total N, OC, P, exchangeable bases and micronutrients, and then field experiments were conducted at two sites located at Makutupora and Hombolo villages. The plant samples were analys`ed for N, P, K, S, Ca, and Zn. The results of soil analysis indicated that all the soils analysed were deficient in N, about 70% had low available P, 90% had low extracted S and 60% had low Ca levels. Also, most of these soils had low Zn levels and only one site had adequate Zn levels. All the soils had adequate levels of Mg, K, Cu, Fe and Mn. The field experiment showed that application of N, P, K, S and Zn increased nutrient contents in sesame at both sites. However, the deficiency was observed in the absolute control treatments. It was further revealed that combined application of sulphur along with Zn and N, P and K significantly increased seed yield and straw dry yield. Following the results from the field experiments, it was concluded that most of the soils under sesame production in Dodoma district that were analysed through this study were deficient in N, P, S and Zn. However, in order to optimize production and increase sesame yield, these nutrients (N, P, S and Zn) must be applied at rates of 45kg/ha, 20kg/ ha, 45kg/ha and 25kg/ha, respectively. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 17 Kalaye J. K. MSc. (2014) Yield and quality of sweet potato as affected by number of nodes buried and potash fertilizers Abstract A study was conducted from January 2013 to June 2013 at Hombolo Agricultural Research Institute in Dodoma, Central Tanzania. The aim was to evaluate response of three sweet potato varieties in productivity and quality to four levels of nodes buried and three potash fertilizer sources. A Split-split plot designs was employed and treatments were applied. Main plot were three sweet varieties, Kiegeya, Mataya and Ukerewe. Sub plot were, nodes burring levels: four buried nodes, (two above ground), five buried nodes (three above ground), seven buried nodes (three above ground) and eight buried nodes (four above ground). The sub-subplot treatments were potash fertilizer sources: Control no fertilizer used, Potassium chloride (KCI), Potassium nitrogen phosphate (NPK) and Farm yard manure (FYM). Results indicated total yield were not affected by varieties planted. There were significant differences among number of nodes on the total yields, the lowest total yield (11.68 t ha-1) was from four buried nodes whereas the highest yield of (15.91 t ha-1) was from eight buried nodes. The application of fertilizers at the rate 120 kg ha-1 significantly influenced the yield components (P <0.05). The lowest number of tuber roots (2.81) was from control treatment while the highest number of tuber roots (6.03) was from KCI treatment. The indicators of tuberous root quality such as diameter (4.81 cm), length (19.03 cm) and weight (0.42 kg plant-1) were from KCI. The total tuber roots yield (18.84 t ha-1) from KCI, (17.51 t ha-1) from NPK, (11.33 t ha-1) from FYM and (8.82 t ha-1) with control treatment. Seven and eight buried nodes with KCI and NPK fertilizers appeared appropriate for optimum sweet potato growth, yield and tuberous root quality in the study area and are therefore recommended. 18 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Wilson, C. MSc. (2014) Molecular characterization of the major histocompatibility complex DRB3.2 locus in indigenous zebu cattle in selected areas of Tanzania Abstract The major histocompatibility complex (MHC) plays important roles in eliciting immune responses and combating infectious diseases. The major histocompatibility complec of cattle is reffered to as Bovine Lymphocyte Antigen (BoLA). The BoLA- DRB3.2 is the most polymorphic bovine MHC class 11 gene which encodes the peptide-binding groove. This gene has been extensively evaluated as a candidate marker for associations with various bovine diseases and immunological traits. In this study, the genetic diversity of the BoLA- DRB3.2 locus was investigated by sequence analysis and comparing the sequences with sequences available in the Genbank. The genetic variability at BoLA- DRB3.2 locus was assessed in 120 cattle belonging to three Tanzanian Shorthorn Zebu strains (i.e. Iringa red (n = 40), Maasai (n =40) and Gogo n = 40) and Fresian breed (n =30). Blood samples were collected from all cows and genomic DNA was extracted from whole blood samples. A two step PCR was carried out in order to amplify a 284 bp fragment of the BoLA- DRB3.2 gene. The nested PCR products were sequenced so as to determine the BoLADRB3.2*polymorphisms. The phylogenetic analysis revealed high percentage variation of BoLA-DRB3.2 locus in Tanzania Shorthorn strains. The phylogenetic tree show that 84% of the BVoLA-DRB3.2 sequences clustered in clade B which include Friesian, Iringa red zebu and Gogo zebu and 16% of the alleles clustered in clade A wjhich include the Maasai zebu only. Based on the genetic distances of the BoLA-DRB3.3 sequences, Gogo and Iringa red zebus were found to be closer to each other (0.0696) while the Maasai and Gogo zebus were the most distantly related breeds (1.1188). This study has revealed that among the four breeds of cattle examined, each breed had a unique allele at BoLA-DRB3.2 locus. Further molecular study is highly recommended to confirm if the different alleles are associated with different adaptive traits. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 19 Kwigizile, O.H. MSc. (2014) Adaptability of selected snap genotypes and RESISTANCE to bean rust (Uromyces appendiculatus (Per.: Pers.) Unger var. appendiculatus) in Southern highlands zone of Tanzania Abstract Snap bean ( Phaseolus vulgaris L. ), also widely known as French bean or Green bean, is of growing importance in the socio-economic systems of East and Central Africa. It is a crop with great potential for addressing food insecurity, improving incomes and alleviating poverty in East and Central Africa. Most of the commercial varieties grown locally are highly susceptible to bean leaf rust caused by Uromyce appendiculatus fungus. Development of disease resistant varieties with acceptable pod quality is a feasible preference to alleviate the constraint. The present work centered on evaluating the adaptability and performance of snap bean genotypes and resistance to bean rust in the Southern highlands zone of Tanzania. Ten advanced bushy snap bean genotypes (HAB 404, HAB 425, HAB 449, HAB 423, HAB 240, HAB 427, HAB 442, HAB 403, HAB 414 and HAB 419), and the check variety Teresa were evaluated for agronomical characteristics, reaction to bean leaf rust, yield potential and pod quality using the Randomized Complete Block Design with three replications, at each location. The plot size was 1.5 m x 1.0 m, with two rows of plants spaced 50 cm x 10 cm. data was collected on growth habit, yield and yield components, and bean leaf rust score beginning 3, 5, and 7 weeks. Pod characteristics (color, shape, length (cm), width (mm) and snap-ability) were also assessed. The entries were scored for the bean rust at vegetative growth, flowering and at pod filling stages. Data was subjected to ANOVA using GenStart 14 Edition (2012) and means were separated using the Fisher’s Protected LSD at P = 0.05. Genotype HAB 427, HAB 425, HAB 423, and HAB 240 were high yielder and showed resistance to bean 20 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS leaf rust. Significant and positive correlations were also observed among genotypes on the yield and yield components. There were highly significant differences at (P≤0.001) across sites on number of pods per plant, total pod yield and pod weight (g), 50% days of flowering and maturity. Interaction between genotypes and location showed also significant differences at (P≤0.01) for the number of pods per plant and total pod yield (kg) per 10 plants harvested. Therefore; genotypes HAB 427, HAB 425 and HAB 240 promised can be used as donor parental materials. Kaswahili, C. R. MSc. (2014) Development of single cross maize hybrids on earliness and yield for lowland areas of Eastern Tanzania Abstract A study was conducted to develop single cross maize hybrids for earliness and yield suitable for the lowlands of eastern Tanzania. The objective of this research was to study the nature of inheritance of earliness in maize genotypes for increased maize yield in the Eastern zone of Tanzania. Seven inbred lines from Chollima Research centre were used in a 7x7 diallel cross to obtain 42 F 1 hybrids. Evaluation of the hybrids was done at three sites of Dakawa, Ilonga and SUA. At Ilonga, the hybrids indicated significant differences for ASI and MC. At SUA only GY of the hybrids showed significance between entries and at Dakawa the hybrids were significantly different on ASI, GW and SW. Across sites the location x entry interaction had significant effects on SW and GY. The entries that gave high yielding across sites were 17, 18, 8, 33, 35 and 40. So these hybrids were recommended for use in the lowlands of Eastern Tanzania to increase grain yield. Inbred lines D and E had good GCA for GY. Lines B and E had good GCA for lateness in AD and crosses with high SCA for lateness in AD such as 19, 7,24, 11, 13, 15, 6 and 38 can be recommended. Inbred lines C and A had good GCA for earliness in SD. Inbred line A had good GCA for shorter ASI. Lines G, C and F had good GCA for R/C while inbred lines A, C,E and F had good GCA for SW. R/C and AD were significantly and positively correlated with yield and their direct effects were high and positive. ASI revealed high negative correlated and direct effect on yield. Research is required to include more diverse accessions and confirm on the performance of the obtained hybrids in future works. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 21 Suvi, W. T. MSc. (2014) Grain yield and quality traits of rice yellow mottle virus resistant rice (oryza sativa l.) genotypes in Morogoro, Tanzania Abstract The study on genetic variability in grain yield and quality of Rice yellow mottle virus (RYMV) resistant rice genotypes was conducted at Sokoine University of Agriculture and Dakawa, Morogoro during 2012 and 2013. Thirty four rice genotypes were laid in a splitpilot arranged in completely randomized design with three replications under screen house condition. These genotypes were screened for resistance to RYMV strains S4 and S5. The results indicated that six rice genotypes including the resistant check, Mwangaza showed resistance to the two strains of RYMV. Field experiments were conducted with six resistant genotypes indentified from screen house experiment plus the resistant and susceptible checks in order to evaluate their agronomic and grain quality characteristics. The experiments were laid out in randomized complete block design with three replications. The analysis of variances indicated significant differences for the some morphological and grain quality traits. The genotypic and phenotypic coefficients of variation were higher for grain yield, amylose content and chalkiness. High broad sense heritability coupled with high expected genetic advance was observed for grain yield, percent filled grains, days to 50% flowering and chalkiness indicating that these characters were controlled by additive gene effects. Correlation coefficients from combined analysis revealed that grain yield was significantly positively correlated with plant height, panicle length, number of grains per panicle and percentage filled grains suggesting their potential contribution to grain yield. Path analysis indicated that, the highest direct effects were observed for percent filled grains and number of grains per panicle on grain yield associated by their respective significant positive correlations suggests importance of their inclusion in selection for the grain yield improvement. 22 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Nkalange, R. H. MSc. (2014) Potential and limitations of contract farming for tea out growers of East Usambara: The case of Amani division, Muheza District Abstract A study was conducted at Amani division in Muheza District, Tanga from 2011 to 2012 to find out the potentials and limitations of contract farming for tea out growers of East Usambara. The survey was conducted in three wards of Amani division which were Mbomole, Amani and Zirai where villages included in this study from these wards were Kwemwewe, Sakale, Mlesa, IBC-Msasa, Makanya, Antakae and Zirai. Therefore a sample of 120 respondents was used to accomplish this study. The respondents were interviewed by using well-structured questionnaire so as to obtain primary data. However, a checklist was used to gather additional information from key informants and Focus Group Discussion (FGD) where observations of farmers circumstances was done by the researcher and a research assistant. Secondary data were obtained from official reports such DALDO’s office - Muheza, Malikitanda Tea Research Institute (TRIT-Maliktanda), Sokoine National Agricultural Library (SNAL), Department of Agricultural Education and Extension (DAEE), Ministry of Agriculture, Food Security and Cooperatives (MAFC). The analysis of data was done by using Statistical Package for Social Sciences (SPSS). escriptive statistics was done to analyze data so as to find the sample means and percentages for studied variables and it was also used for drawing frequency distribution graphs and tables. The findings from the study revealed that there were potentials which exist to Amani tea out growers producing under contract farming despite of the limitations under the contractual arrangement. However, the findings have revealed that farmers have engaged in tea out growing due to availability of market for their produce and fringe benefits they acquire when producing under contract. Therefore contract farming can provide a win situation though wins most is not always predictable. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 23 Hiza, S. J. MSc. (2014) Livelihood implications of agricultural lan d loss through urban expansion and population growth in Kibaigwa area in Dodoma, Tanzania Abstract The study conducted in Kibaigwa a rapidly expanding Township in Kongwa District, Dodoma, Tanzania. Objective of the study was to examine livelihood implications of agricultural land loss through urban expansion and population growth. A number of 120 households randomly selected interviewed using structure questionnaire. One session of informal group discussion conducted among community elders to investigate the changing of land use that is taking place. Results indicated that production system of Kibaigwa inhabitants was slowly changing to non-farm production and some of the observed examples were maize and sunflower seeds processed to flour and oil respectively. The analysis also showed decrease of agriculture land from 82.2% in year 2000 to 64.8% in year 2010 and thus leaving 38% of population depending in agriculture for livelihood with agricultural land of 1 to 5.5 acres per household. Selling of agricultural lands observed to contribute loss of agricultural land and regression analysis indicated that location of agricultural land from the main road and education level of the farmer contributed to rapid selling of agricultural lands. Agricultural lands converted to settlements and other urban infrastructure were directly related to increase in population and using GIS, results indicated that about 27% of agricultural land converted to settlements and other urban infrastructure between 2000 and 2010. Urban land use coefficients estimated to provide a measure of the amount of land converted to urban uses per person added to the population. The coefficients indicated that between 2000 and 2010, for every one-person increase in population about 0.18 hectares of agricultural lands converted to settlements and other urban infrastructure. Both agriculture and urban development are inevitable, the findings can be useful looking for ways to balance between urban expansion and preservation of agricultural land to improve livelihood of the people. 24 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Maganga, R. M. MSc. (2014) Population structure of pyricularia grisea and reaction of rice cultivars in the Southern highlands of Tanzania Abstract The study was designed to investigate the diversity of Pyricularia grisea (Coke) Sacc, population structure and reaction of rice cultivars in Southern highlands of Tanzania. Screen house experiments and a field trapping nursery were used to determine prevalent pathogenic races, effective host resistant genes and reaction of rice cultivars against identified pathogenic races. A total of six pathogenic races U11-i2-k141-z13-ta 103, U51i4-k100-z13-ta103, U11-i4-k041-z11-ta103, U11-i5-k000-z030ta000, U11 –i0-k101-z13ta100 and U11-i1-k120-z11-ta102 were identified on the basis of the infection type of LIJIANGXINTUAN HEIGU (LTH) monogenic lines. Pathogenic race U11-i2-k141-z13-ta103 was the most virulent strain infecting 44.4% of the tested varieties, followed by U51-i4k100-z13-ta103; U11-i4-k041-z11-ta103; U11-i1k120-z11-ta102;U11-I0-K101-Z13-ta100 and U 11-i5-k000-z03-ta000 infecting 40.7, 37, 37, 33.3 and 14.8%of the tested varieties, respectively. Host resistance genes Pi12, Piz-t, Pi11, Pik-m, Pi12, Pia and pik-p were found effective against the available pathogen races. All local varieties tested were susceptible to all races identified differing only on the levels of susceptibility. Uyole inbred lines (UR 319 and UR 400) were susceptible to all races except race U11-i5-k000-z03-ta000 and race U11-i4-k041-z11-ta103. The races identified provide an insight of blast population diversity prevalent in Southern highlands that should be considered when breeding for blast resistance. Effective resistant genes identified can be pyramided to Uyole inbred lines and commonly grown local susceptible rice varieties to improve blast resistant against prevalent races in Southern highlands. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 25 Rwehumbiza, J. M. MSc. (2014) Evaluation of raw cow milk quality Tanzania production in Bagamoyo and Kisarawe districtris, Tanzania Abstract A cross sectional study was conducted to evaluate milk quality supplied to collection centres between January and June 2012 in Bagamoyo and Kisarawe districts, Pwani region. A total of 186 samples were collected and tested for organoleptic properties, alcohol, clot on boiling (COB), milk density (lactometer) and butterfat (BF); total bacteria count (TBC) and Coliform counts. The presence of antibiotic residues was also assessed. Questionnaires were administered to 93 farmers to identify management factors which may affect milk quality. Milk collection centers (MCC) were visted twice; at the start and end of rainy season. All samples had normal organoleptic properties and negative to alcohol and COB tests. Results on milk density indicated similar (P>0.05) distribution of milk grades between districts and seasons with lactometer reading means of oL26.55≠1.34≠1.46 for Bagamoyo and Kisarawe districts, respectively. Lactometer reading means for season were oL26.71≠1.17 and oL26.50≠1.59 at the start and end of rainy season, respectively. BF distribution was similar (P>0.01) between districts but significantly different (P<0.01) between seasons. Overall percentage means were 3.97≠0.84 and 4.14≠0.88 for Bagamoyo and Kisarawe districts respectively; and 4.31≠0.85 and 3.77≠0.78 at the start and end of rainy season respectively. LogTBC and Log Coliform counts were significantly different between districts and seasons (P<0.01). Analysis of variance (ANOVA) indicated that source of water, means of transport and animal feeding methods contributed significantly to milk bacteria load whereas t-test indicated that housing system also had significant effect on TBC and Coliforms (P<0.01). Positive samples to antibiotic residues were 21% although there was no significant difference between locations and seasons. It is concluded that milk produced in Bagamoyo and Kisarawe districts was of poor quality which is of public health significance. 26 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Chasama, G. L. MSc. (2013) phenotypic characterisation of Ukerewe and Bunda Tanganyika shorthorn zebu cattle in the Lake Victoria basin Abstract A study was carried out to characterise phenotypically the Ukerewe TSZ strain in order to know if it differs from the Bunda TSZ strain. Respondents from 46 households were interviewed on sources of income and food, number and species of livestock kept, ranking of livestock, usage of cattle, age at first calving, calving interval and lifetime number of calving. A total of 169 adult TSZ cattle were examined for coat colour pattern, and colours of coat, skin, muzzle, eyelid and hoof. Other traits that were examined are horn shape and sizes of udder, dewlap, testicle, navel-flap and prepuce. Physical body measurements namely body weight, heart girth, body length, withers height, horn length, ear length, muzzle circumference, hock circumference and tail length were taken for each cattle. The SPSS and SAS computer packages were used to analyse qualitative and quantitative data, respectively. Ukerewe TSZ cattle were observed to be significantly (p<0.05) heavier and calving earlier than Bunda TSZ cattle, whereas calving interval and lifetime number of calving revealed insignificant (p>0.05) differences between the two strains. Black colour occurred at significantly (p<0.05) higher frequency for the Ukerewe TSZ strain than for the Bunda TSZ strain. The Ukerewe TSZ strain was also found to have significantly (p<0.05) bigger heart girth and shorter horn length than the Bunda TSZ strain. The rest of the physical measurements and all external body appendages showed insignificant (p>0.05) differences between the two strains. With the exception of ear length and muzzle circumference, all measurements were positively and highly significantly (P<0.01) correlated with the body weight estimated from heart girth. Thus, the strains under the study differed remarkably in phenotype. Advanced characterisation of the strains using microsatellite DNA markers is recommended in order to establish if they could have a common origin. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 27 Mohamed, A. MSc. (2013) Developmental biology of fruit fly (diptera: tephritidae) species attacking cucurbits Abstract Fruit flies are among the major pests of cucurbits in many parts of Tanzania. Studies on fruit flies (Diptera: Tephritidae) attacking cucurbit in Morogoro were conducted at Sokoine University of Agriculture (SUA). Objectives of the studies were: to compare incidences and infestation rates of selected fruit fly species in different stages of cucumber fruit, to establish duration and determine survival of immature stages of Bactrocera cucurbitae in selected cucurbitaceous hosts. Cucurbit fruit fly species incidences and infestation rates were determined from fruit flies emerging from fruits harvested in crop museum using standard procedures. Duration and survival of B. cucurbitae immature stages were established and determined respectively by observing B. cucurbitae immature stages development in watermelon, cucumber and pumpkin at 20, 25 and 30oC using environmental chamber. High incidence (0.75) was observed in immature cucumber fruits with B. cucurbitae while low (0.16) was in mature cucumber with Dacus frontalis. High infestation rate (120 flies/kg fruits) was observed in immature fruits by B. cucurbitae and low (5 flies/kg fruits) was in mature fruits by D. frontalis. Long duration (16.23 days) of B. cucurbitae was recorded in pupal stage at 20oC while short duration (0.99 days) was in egg stage at 30oC. High (97.72%) and low (76.03%) survivals of B. cucurbitae was recorded in egg stage at 30oC and 20oC respectively. Among targeted fruit flies B. cucurbitae had high incidences and infestation rates in cucumber fruit stages. Duration and survival of B. cucurbitae immature stages decreased and increased respectively with increase in temperature. Further studies on incidence and infestation of fruit flies in different cucurbitaceous hosts, agro ecological zones and seasons should be conducted. Relationships on biology of D. bivittatus and D. frontalis with temperature need to be studied. The studies will add information on rearing, forecasting and ecological management of fruit flies. 28 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Kalloka, M. S. MSc. (2013) Phenotypic characterization of indigenous chicken ecotypes in southern Tanzania Abstract Indigenous chickens are of great economic and social importance in Tanzania, however little information is available on their phenotypes, productivity and genetic potentials. In this study, 125 adults indigenous chickens were obtained from three agro-ecological zones of Southern Tanzania, namely coastal, lowland and Makonde Plateau represented by Kilwa district, Nachingwea district and Tandahimba district respectively. Egg physical characteristics like (egg weight, egg color) were collected from 100 indigenous chickens at Livestock Research Centre-Naliendele. Quantitative traits like adult body size, qualitative traits like plumage color and other physical characters were used to divide the chickens into five convenient ecotypes namely, Kuza, Kishingo (bared neck), Njachama (frizzled), Sukuma, and Vibete (dwarf). Differences in adult body weight, wing span, body length, shank length, leg length, plumage colour, comb shape, shank feather, shank colour, earlobe colour and skin colour were assessed. Njachama and Sukuma cocks were significantly heavier compared to other ecotypes (p<0.05) while the Sukuma and Kuza hens were heavier than other hens (p<0.05). On average the Njachama and Sukuma cocks weighed 2039.16g and 2004.16g while the Sukuma and Kuza hens weighed 1661.53g and 1556.92g respectively. Eggs from Sukuma and Kishingo had average weight of 44g each and, were significantly heavier (p<0.05) than those from the other ecotypes which weighed on average less than 40g in weight. Comb type varied from single to rose, while earlobe colour varied from red white to pink. Variations in shank colour were from grey, yellow to black and skin colour was either yellow or white. This study has demonstrated that the indigenous chickens in Southern Tanzania have variations in phenotypic and production characteristics (e.g growth rates) that can be used as tools for selective breeding programmes aimed at boosting productivity of indigenous chickens in Tanzania. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 29 Kadege, E.L. MSc. (2013) Prevalence and control of seedborne fungal pathogens of wheat in farmers saved seeds of selected locations in northern Tanzania Abstract Seed health testing to detect seed-borne pathogens is an important step in the management of crop diseases. Laboratory and screen house experiments were carried out at Selian Agriculture Research Institute Arusha, to investigate the extent of seedborne fungal pathogen problems from farmers saved seeds in Northern wheat growing Zone. A total of 45 untreated seed samples of wheat were collected from farmers saved seeds in Karatu, Hanang and Siha Districts. Each sample was physically inspected and pure seeds were separated from abnormal seeds and inert matter. Seed samples collected at Siha were of high quality compared to seed samples collected at Hanang and Karatu. Pure seeds ranged from 98.2–99.2%, abnormal seeds 0.49– 1.4% and inert mater 0.15–0.80%. Alternaria alternata, Bipolaris sorokiniana, Dresclera tritici, Fusarium graminearum, Fusarium moniliforme, Aspergillus flavus, Cladosporium sphaerormum, Epicoccum purpurascers, Pyricularia oryzae and Penicilium Corylophilum were isolated and identified using blotter, potato dextrose agar (PDA) and agar plate methods. The most predominant seed-borne fungi were Cladosporium sphaerormum (9.8%), Alternaria alternata (9.2%) and Aspergillus flavus (8.7%). The lowest percent seed infection (10.5%) was recorded in samples collected at Siha District, (17.5%) at Hanang District and (20.4%) at Karatu District. The percentage fungal seed infection was higher in seed samples collected at Karatu with infection rate of 32.8% and lowest for seeds sample collected at Siha with infection of 25.4%. The overall seed infection in the region was 29.1% causing yield losses of 1.2 t/ha on average. Seed treatment with Metalaxy plus, Mancozeb and Baytan was found to increase percent seed germination by 17.2, 14.3, and 12% respectively and yield by 27.8, 20.4 and 17.8% respectively. Farmers saved seeds in Hanang, Karatu and Siha districts are heavily infected with fungi and it is recommended that they should be treated with appropriate fungicides prior planting. 30 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Vicent, E. MSc. (2013) Evaluation of genotype x environment interaction on yield components of tomato (solanum lycopersicum l.) breeding lines and participatory selection Abstract Genotypes x environment interaction (G x E) studies were conducted on fruit yield and yield components while considering farmers preferences in identification of superior genotypes. Site used were Madiira, Kilema pofu and Msitu wa tembo. Thirteen (13) tomato genotypes were assigned in a randomized complete block design with three replications under split plot design. Study results reveals that, genotypes TeTo 11 14-1, TeTo 11 13-1 and TeTo 11 03-1 had high mean yields of 15.21, 15.30 and 13.85 tone/ha respectively across all locations. They showed best performance on number of fruits per plot and fruit weight per plot in combined analysis. In participatory analysis, disease resistance, drought tolerance and number of pickings were the best selection criteria at Kilema pofu. Genotypes TeTo 11 14-1, TeTo 11 02-1 and TeTo 11 03-1 were selected as the preferred genotypes at this location. The best selection criteria for farmers at Madiira were drought tolerant, fruit size and disease resistance, the most preferred genotypes at this location was TeTo 11 13-1 and TeTo 11 06-1 At Msitu wa tembo, the best farmer’s selection criteria were drought tolerant, Skin hardness and fruit shape while preferred genotypes were TeTo 11 14-1and TeTo 11 13-1. Disease resistance and drought tolerant were the best selection criteria across all three locations. Genotype and genotype x environment interaction (GGE) biplot stability analysis revealed that genotype TeTo 11 03-1 and TeTo 11 14-1 were more stable than others. Path coefficient analysis showed that, plant height and number of fruits per plant were the yield components that can be used to improve tomato yield both at genotypic and phenotypic levels. Fruit number and fruit size interacted negatively in the relationship between these variables and yield. The analysis revealed the importance of compensation effects of yield components in a system of variable interactions. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 31 Furahisha, E. H. MSc. (2013) Farmers adoption of selected recommended rice production practices: A case of Kilombero District of Morogoro Region, Tanzania Abstract None or low adoption of recommended rice production practices like recommended fertilizer and recommended rice varieties have influenced to conduct this study. The study investigated the adoption level and factors that influence the adoption of recommended fertilizer package and recommended rice varieties in Kilombero District. Primary data were collected by the use of pretested interview questionnaire from 120 respondents selected at random to represent farmers of Kilombero District. Data were analyzed by using statistical package for social science (SPSS 16.0) computer program, where frequency and percentage were used to determine distribution of the study variables. Correlation was used to determine relationship between independent and dependent variables while Chi – square tested the significance difference between variables. Findings reveal that the level of adoption of recommended fertilizer package and recommended rice varieties in the study area is low. Respondents who applied fertilizers (68.4%) applied at different levels, that is (21.7%) fall under low adoption level, (20.0%) fall under medium adoption and (6.7%) of the respondents fall under high adoption level. Only (43.3%) of the respondents planted recommended rice variety that is TXD 306, the rest of respondents planted other rice varieties which are local and improved but not recommended. Several factors influenced the adoption and these are the independent factors like area under rice and the intervening factors namely the Efficiency Misperception (EM), Need tension (NT), Awareness and Prominence. Other factors that hinder adoption of recommended fertilizers includes, destroys soil, high cost and affect flowering and for recommended rice variety includes, susceptibility to diseases, needs great care, poor market and much water is needed. In general, the adoption of recommended fertilizer package and recommended rice variety in the study area is strongly influenced by the intervening variables. Therefore the intervening variables which need to be focused in enhancing the adoption of recommended fertilizer package and recommended rice variety in the study area are need, perception and knowledge. 32 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Kindimba, G. V. MSc. (2013) Effect of benzlyaminopurine on in vivo multiplication and genetic stability of plantain (musa spp. AAB) cv. ‘itoke sege’ Abstract In vivo macropropagation either alone or in combination with benzlyamimopurine (BAP) is an alternative simple technique for banana multiplication but has not been applied to recalcitrant plantain such as cv. “Itoke Sege”. This study was conducted to determine the effect of BAP concentration on in vivo multiplication and genetic stability among in vivo derived regenerants of plantain cv. ‘Itoke sege’. An experiment was laid out in RCBD with four treatments each replicated three times. The treatments consisted of four BAP concentrations (1.5, 3.0, 6.0 mg/l and untreated control). Data were collected on number of days to first shoot emergence, number of shoots per corm, shoot size and morphological and genetic stability of in vivo derived shoots. Morphological stability was assessed using banana morphotaxonomic descriptors while genetic instability was assessed based on analysis of 2C nuclear DNA content of in vivo derived suckers. Results showed that BAP concentration at 1.5 mg/l significantly (P ≤ 0.05) enhanced first the shoot emergence at 15.78 days followed by BAP at 3.0, 6.0 mg/l and untreated control with 25.18, 28.39 and 36.43 days, respectively. Moreover, BAP concentration at 1.5 mg/l significantly (P < 0.05) produced the largest number of suckers of 17.11 suckers per corm followed by untreated control and BAP concentration at 3.0 and 6.0 mg/l and with 15.23, 13.08 and 12.96 suckers per corm, respectively. Similarly, BAP at 1.5 mg /l and untreated control significantly (P ≤ 0.05) showed the lowest frequencies of off-types with 10.89 and 10.23 % compared to BAP at 3.0 and 6.0 mg/l with 12.08 and 12.86 % of off-types, respectively. However, ploidy analysis revealed that the off-type and normal banana suckers had significantly (P ≤ 0.05) equal 2C nuclear content and ploidy level. The findings of this research provide evidence for the use of in vivo macropropagation coupled with BAP at 1.5 mg L-1 as an alternative technology for rapid production of planting materials of recalcitrant plantain varieties. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 33 Makuru. H. D. MSc. (2013) Heritability, stability parameters and paths of influence on yield components of sesame (sesamum indicum l.) Breeding lines Abstract Field trials were conducted during the rainy season of 2012 to study the heritability, stability and contributions of some yield attributes to the seed yield of 9 sesame genotypes grown in two spacings at Naliendele, Nachingwea and Tunduru. The experiments were arranged in a split-split plot design in three replications at three sites. Observations were recorded on days to 50% flowering, total plant height, height to first branch, height to first capsule, number of primary branches, capsule on the main stem, number of capsules per plant, leaf spot score, date of harvesting, seed yield and 1000 seed weight. The genotypes varied considerably in the two spacings and GEI was highly significant. The traits, number of first branch, number of capsules per plant and seed yield showed high PCV and GCV estimates. There is scope for selection based on these characters, and the diverse genotypes can provide materials for a sound breeding programme. Most of the characters had low heritability along with low genetic advance; such situation may arise due to nonadditive gene action indicating greater influence of environment in the expression of these characters. Seed yield per plant showed positive correlation with days to 50% flowering, total plant height, height to first branch, height to first capsules and capsule per plant. 1000 seed weight showed negative association with seed yield per plant. Genotype Nal. 06. Sc 31 was high yielding and found to suit in more favourable environmental conditions while Mtwara 09 showed to have good adaptability under more unfavourable conditions. According to this experiment, it was concluded that specific genotypes for specific environments are needed in order to maximize grain yield in sesame. 34 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Hashim, I. MSc. (2013) Banana xanthomonas wilt: incidence, transmission, pathogen characterization and management options in Kagera, Mwanza and Mara Regions Abstract Field surveys were conducted from December, 2011 to January, 2012 in Kagera, Mwanza and Mara regions to assess the current status of banana Xanthomonas wilt (BXW) disease. Twenty eighty banana samples with BXW disease symptoms were randomly collected from a total of 147 surveyed fields. Farmers’ knowledge of BXW disease was assessed using a checklist, discussions and direct field observations. Isolation of bacteria was done on Yeast Peptone Glucose Agar and identification was done based on morphological, biochemical, polymerase chain reaction (PCR) and pathogenicity tests. Results indicated that the incidence of BXW disease was highest (56.7 %) at Ihangiro and the lowest (10 %) at Ruhija and Mulela, in Muleba District; Nakamwa and Busagami, in Ukerewe District. Such results implied that, BXW disease is a constraint to banana production in the surveyed districts. The results also indicated that BXW pathogen transmission was through infected farming tools (65.4 %) in Muleba District and infected planting materials in Tarime (50.5 %) and Ukerewe (45.8 %) Districts, implying that these were the major means by which BXW disease was spread in the study area. About 58.33 % and 41.67 % of farmers at Ibare and Kishanda villages, respectively, in Muleba District farmers associated pied crow (Corvus albus L.) with the transmission of BXW causing pathogen. Results based on morphological, biochemical and PCR test indicated that, four out of 16 bacteria isolated from infected banana samples were most likely Xanthomonas campestris pv. musacearum (Xcm). This study recommends farmers training through seminars on BXW disease identification and proper management. Further studies on the relationship between the pied crow and Xcm transmission in Kagera, Mara and Mwanza regions, are also recommended. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 35 Ismail, M. MSc. (2013) Morphological and molecular characterization of finger millet genotypes collection in Tanzania Abstract The availability of diverse genetic resources is very essential for genetic improvement of any crop including finger millet (Eleusine coracana L). This study was carried out to assess the morphological and genetic diversity of Tanzanian finger millet germplasm. A balanced lattice field experiment accommodating 196 accessions was laid in three replications at Makutupora Agricultural Research Institute, Dodoma Tanzania. Morphoagronomic traits were evaluated and scored based on International Board of Plant genetic Resources (IBPR) descriptors. Data obtained on morphological traits were used to calculate for principal component analysis, similarity matrix and cluster analysis using Genstat 14 edition computer software. In morphological characterization total number of tillers, number of productive tillers and number of days to heading showed high discriminatory ability, these traits contributed highly (24%) of the total variation among individuals. While molecular characterization was carried at ICRISAT Molecular laboratory, Nairobi – Kenya. A total of 20 SSR markers were used in determination of genetic diversity of these 196 finger millet accessions where 19 SSR markers were polymorphic and could amplify corresponding polymorphic alleles among genotypes with the average number of alleles amplified by each marker being 22 within accessions. These 19 SSR markers were highly polymorphic with their polymorphic information content (PIC) ranging from 0.55 to 0.93 with an average of 0.82. Both dendrogram and cluster analysis generated from these allelic and morphological data clearly discriminated these finger millet genotypes into five groups. Data generated from these studies suggests that Tanzanian finger millet genotypes are rich in both morphological and genetic diversity. 36 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Joachim, J. S. MSc. (2013) Genotype x environment interaction in cowpea (Vigna unguiculata-l. Walp.) for yield performance in the Lake Zone of Tanzania Abstract Cowpea (Vigna unguiculata L.Walp.) (2n=2x=22) is a member of the Phaseoleae tribe of the Leguminosae family. Cowpea is an important grain legume as well as fodder crop, especially in the dry regions of Africa. The production of this crop has been below average because of low genetic variation and cultivation of poor-yielding varieties which have not been improved. Genotype by environment interaction studies were conducted in Misungwi, Bariadi and Maswa locations on ten cowpea genotypes in a randomized complete block design under split plot experiment for grain yield and other growth characters. Significant mean values under combined analysis were observed with their mean range; germination percentage (83.61 to 99.44%), initial plant stand (34 to 40), days to 50% flowering (43 to 62 days) and final plant stand (34 to 40) in which the outstanding performance for the preceding characters was observed on IT99K-1122. Mean number of pods per plant ranged from 6 to 10 pods, whereby, VULI-1, VULI-2, TUMAINI, FAHARI, and IT99K-573-1 had better performance for this character, for the mean number of seeds per pod which ranged from 10 to 16 seeds, the outstanding performance was recorded on VULI-2, Mean weight of 100 seeds ranged from 10.68 to 20.22 g, IT99K-7-21-2-2 had the highest performance for this character. Moreover, the mean grain yield ranged from 1648 to 2379 kg/ha, TUMAINI revealed outstanding performance for this character and the LOCAL VARIETY was the lower performing genotype. Participatory variety selection was done at each location during flowering and at harvest. Farmers identified IT99K-1122 as the best genotype during flowering at Misungwi and Bariadi, while at Maswa IT99K-7-21-2-2 was recorded as the best. PVS at harvest recorded IT99K-1122, (LOCAL VARIETY and IT00K-1263), and LOCAL VARIETY for Misungwi, Bariadi, and Maswa respectively. The partial (farm) budget revealed the net income of Tsh 2 537 200/= per hectare of land grown cowpea as the profit made. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 37 Kalimba, H. F. MSc. (2013) Effect of herbicides and soil moisture on weed control, yield and quality of sugar cane (Saccharum officinarumL) at Kilombero, Tanzania Abstract Field experiments were conducted at Kilombero Sugar Company estate one under rainfed and another under rainfed plus supplemental irrigation condition to evaluate the effects of herbicides on weeds and sugarcane (Saccharum officinarum L) yield and quality. Experiments were laid out in a randomized complete block design with four replications. Plot sizes of 5.8 m x 4 m were used. Treatments were Volmazone (2-(2-chlorobenzyl)4,4-dimethyl-1,2-oxazolidin-3-one) at 2.9 and 3.8 kg a.i ha-1; Acetochlor 2-chloroN-ethoxymethyl-6-ethylaceto –o-toluidide at 4.5 and 6.0 kg a.i ha-1 and Metribuzin 4-amino-6(1,1-dimethyl)-3-Methilthio-1,2,4 triazin-5(4H) 6.0 and 7.5 kg a.i.ha-1. Which were applied as early post emergence. These were compared with weed free check which was hand weeded every two weeks and untreated check. Results revealed that there was significant difference between herbicides treated plots as compared to unweeded check plots (p < 0.05). There was no significant difference between herbicide treated plots and weed free check. All herbicides were effective in controlling grasses and broadleaf weeds to an acceptable level above (90%) up to 8 WAT for both irrigated and rainfed crop, a period which is quite satisfactory for sugarcane. There were variable herbicide treatment effects on individual grass weed species. Itchgrass was best controlled by Volmazone, while Leptochloa and Echnochloa spp were best controlled by acetochlor and metribuzin. Almost all herbicide treatments were ineffective incontrolling nut sedges the control was bellow an acceptable level 90%, although were significant (p < 0.05) as compared to unweeded check. There was a significant difference in sugarcane yield tons ha-1 between herbicides treated plots as compared to unweeded check plots (p < 0.05) for both irrigated and rainfed experiments,but no significant difference in sugarcane yield was observed between herbicide treated plots and weed free check (p < 0.05). 38 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS For the irrigated experiment, the treatment with acetochlor 4.5 kg a.i ha-1increased yield by 74.7 % which was higher than all other treatments. Other treatments, volmazone 3.8 kg a.i ha-1; metribuzin 6.0 kg a.i ha-1;volmazone2.9 kg a.i ha-1; metribuzin 7.5 kg a.i ha-1; weed free check and acetochlor 6.0 kg a.i ha-1 increased yield between 37.8 and 57.3 % compared to unweeded check. For the rainfed experiment, treatment with acetochlor 4.5 kg a.i ha-1increased yield by 50.0 % and other treatments that is acetochlor 6.0 kg a.i ha-1; metribuzin 6.0 kg a.i ha-1; weed free check; metribuzin 7.5 kg a.i ha-1; volmazone 3.8 kg a.i ha-1 and volmazone 2.9 kg a.i ha-1 increased the yield of sugarcane between 40.3 and 48.8 % over unweeded check. Both irrigated and rainfed experiments sugarcane yields tons ha-1 positively correlated with number of millable stalks population (r = 0.834 and 0.942) respectively. Different treatments of herbicides had no effect on sugarcane quality parameters (P < 0.05). Economic analysis showed that the highest net benefit and marginal rate of return of 1124.2% was achieved by treatment with volmazone at 2.9 kg ai ha-1for irrigated experiment and for the rainfed experiment the highest net benefit and marginal rate of return of 412.3 % was achieved by treatment with acetochlor at a rate of 4.5 kg a.i ha-1. Kapeleka, J. A. MSc. (2013) Effectiveness of guidelines on pesticide labels as a communication tool for smallholder farmers on pesticides handling in Northern Tanzania Abstract Intensified agricultural production means increased pest problems necessitating pesticides use, hence the need for effective use of pesticide labels in communicating pesticides use information. This study assessed the effectiveness of pesticides label use in communicating useful information to horticultural farmers from Mang’ola and Ngarenanyuki, Karatu and Arumeru districts respectively. A total of 200 respondents were obtained through simple random selection. Data were collected through personal and face-to-face interview using structured interview schedule, observation and focus group discussion. Simple descriptive statistics and cross tabulation were used in the analysis. The results show that pesticides labels do not communicate fully the intended information on pesticide use. Majority (79.6%) of farmers interviewed had not learned anything from the labels. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 39 The main source of information on pesticides use was pesticides retailers. Even when farmers read instructions, most farmers do not understand the instructions provided on pesticides labels as most of the guidelines and instructions are given in highly technical nature, foreign languages, incomplete statements, and/or uncommon colour codes, symbols and pictograms. Factors influencing the use of information on pesticide labels were found to be farmer’s belief that following instructions leads to low pest mortality, lacking emphasise and sensitization on label use and reading habit. It is recommended that training on pesticides safety and pesticides label use be implemented to farmers. Rules and regulations governing pesticides registration and business should be enforced. Pesticide labels need to be simplified and capacity building on symbols, colour codes, and pictograms safety precautions, pesticides costs, health and environmental impacts of pesticides use is highly required to pesticide users. Kilambo, D. L. PhD (2013) Responses of compact coffee (Coffea arabica) clones to coffee berry disease and leaf rust under varied environmental conditions in Tanzania Abstract INTRODUCTION: Several introductions of Arabica coffee into Tanzania have been made since 1880’s, but it was until 1935 when serious selection of improved cultivars started, that is, shortly after the establishment of the Coffee Research Station at Lyamungu. This eventually resulted in several cultivars, of which N39 (Bourbon) and KP423 (Kent) became the most widely planted. These cultivars have not only been well adapted across coffee growing regions in Tanzania but they also produce fine quality coffees, which are in high demand by buyers and roasters. However, they are highly susceptible to coffee berry disease (CBD), caused by Colletotrichum kahawae Waller and Bridge Sp. Nov., and coffee leaf rust disease (CLRD) caused by Hemileia vastatrix Berk et Br. Coffee berry disease can destroy 30 to 90% of the crop, while CLRD infection can cause severe leaf defoliation leading to die-back of primary branches and lowering of yields followed by death of the coffee tree. 40 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS The two diseases have also a profound effect on the coffee quality. When CBD attacks berries at ripening stage it causes brown lesions which stain the parchment and the green beans. Defoliation by CLRD infection could negatively affect berry development resulting into small and poorly filled beans, which produce an inferior beverage. Breeding of coffee varieties with resistance to CBD and CLRD is one of the strategies for effective management of the diseases. In recent years, TaCRI has developed a series of compact coffee breeding lines in which some of them were tested under varied environmental conditions. The objective of this study was to evaluate the performance of compact coffee hybrid clones of Arabica coffees for resistance to CBD and CLRD, yield and quality in different ecosystems in Tanzania, with the aim of recommending compact coffee clones which are suitable in specific or across ecosystems. INNOVATIVE FINDINGS: Compact coffee genotypes tested in areas prone to CBD and CLRD under varied environmental and controlled conditions showed resistance to the two diseases. Outstanding performance was shown by compact coffee genotype CVT14 which showed CBD and CLRD resistance under natural and controlled condition. In this study, compacts were shown to have yield potentials of as high as 7000 Kg/ha. Almost all the 16 compact coffee genotypes tested for beverage quality, qualified for export value, with CVT6 scoring class 4 described as of specialty coffee. In this study, seven (7) new races of Hemileia vastatrix a causative agent for CLRD were identified. Also aggressive strain of Colletotrichum kahawae was confirmed; strain 2006/14. USEFULNESS OF THE FINDINGS: i. Compact coffee genotype CVT14 has shown the highest resistance degree to CBD and CLRD, yields of more than 2000 Kg/ha of green beans, and beverage characteristics for export value. This can be recommended for commercial use by the coffee growers. ii. Compact coffee genotype CVT6 has shown to inherit fine flavour quality, but lacks some disease resistance ability. It is recommended to backcross with coffee genotypes of attributes to disease resistance to add its strength NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 41 IMMEDIATE APPLICATION OF THE FINDINGS: i. Recommendation of compact coffee genotype CVT14 for its official release, multiplication and distribution of the variety to coffee growers. ii. Continue to update information on existing races of H. vastatrix and strains of C. kahawae in coffee growing areas. iii. Use of C. kahawae strains 2006/14 in screening for CBD resistance because of its aggressiveness. Kissimbo, M. M. MSc. (2013) An assessment of public-private-partinerships in provision of agricultural extension services in Mtwara Abstract Over the years the provision of extension services in Tanzania was dominated by the public sector. However, effectiveness of the public sector extension has declined greatly during the past decade. In recent years several private extension providers have emerged to fill the gap created by reduced presence of public sector extension. Thus, this study was conducted to assess Public-Private-Partnerships (PPPs) in provision of agricultural extension services in Mtwara District, Tanzania. Specific objectives were to: identify types of parties involved in PPPs in the study area; determine different roles the identified parties play; and examine forms of PPPs partnerships. Data were collected from 154 respondents, including 120 household heads, four extension agents, 30 key informants and various documentary sources using questionnaires, researcher’s diary and checklist. Quantitative data were analysed by using Statistical Package for Social Sciences (SPSS) computer programme and qualitative data were analysed using “content analysis” technique. The study identified types of PPPs involved in the provision of agricultural extension services, different roles played by identified PPPs parties and forms of PPPs partnerships. It was concluded that the role of government in PPPs is to build an environment in which plurality of extension providers can thrive. Special attention should be given to those service providers who have vested interest in long-term success of services they are providing. 42 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS It was therefore recommended that a loose network of formal association of private service providers is deemed necessary for any interaction with the government. The study also suggested undertaking studies on forms of PPPs in provision of extension services in other parts of the country in order to enable generalisation of observations. Ndomba, M. D. MSc. (2013) Evaluation of soil fertility status and response of maize to different nutrients in selected soils of Tabora District Abstract A study was undertaken to assess the soil nutrients status on selected maize growing areas and determine combinations of nutrients to optimize yield. Soil samples were collected from10 villages where maize is the main food and cash crop. These areas were; Kipera, Lolanguru, Itema, Nyangahe, Farmnyamwezi, Malolo, Mpenge, Mawiti, Lyaudusi and Msange. Two composite samples were made from each village resulting to 20 samples. Field experiment was conducted at two villages (Kipera and Nyangahe) to determine combinations of nutrients for optimal maize yield. Factor (a) – main plot was three maize varieties (Staha, TMV1 and Situka M1) and factor (b) – subplots comprised six nutrients treatments namely; an absolute control (T1), nitrogen alone at 100 kg N ha-1 (T2), nitrogen at 100 kg N ha-1 and phosphorus at 40 kg P ha-1 (T3), nitrogen at 100 kg N ha-1, phosphorus at 40 kg P ha-1 and 100 kg K ha-1 (T4), nitrogen at 100 kg N ha-1, phosphorus at 40 kg P ha-1, 100 kg K ha-1 and 20 kg S ha-1 (T5) and nitrogen at 100 kg N ha-1, phosphorus at 40 kg P ha-1, 100 kg K ha-1 and 20 kg S ha-1 and 10 kg Zn ha-1(T6), were tested in split plot design in randomized complete block arrangement, replicated three times. Results obtained from Kipera and Nyangahe villages, showed that fertilizer types applied and their combination (T6) produced significantly (P<0.05) higher maize grain yield over T1, T2 and T3. Also Staha and TMVI showed better results than Situka M1 at both sites. Overall the study results indicated that the fertilizer combination in (T6) at 100 kg N ha1, at 40 kg P ha-1, 100 kg K ha-1 and 20 kg S ha-1 and 10 kg Zn ha-1 could be the best strategy in improving maize grain yield. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 43 Mbegu, S. J. MSc. (2014) Developing top cross maize (Zea mays L.) hybrids tolerant against maize streak virus (msv) disease for the Eastern Zone Ecologies Abstract Maize production in smallholder farming community has remained low due to a number of constraints which include biotic and abiotic. Among biotic constraints, Maize streak virus (MSV) disease is one of the major causes of low yield if not controlled. Genetic resistance appears to be the best economical option to control MSV and it is readily adopted as method for smallholder farmers, because of less cost and environmentally friendly. The main objective of this study was to produce high yielding top cross maize hybrids which are tolerant to MSV disease for growing in the Eastern ecologies of Tanzania. Staha (female) and fourteen inbred lines (male) were used to produce top cross hybrid genotypes. Evaluation for MSV resistance was done under natural infection. RCBD replicated three times was used in three selected MSV hot spots of Eastern areas of Tanzania. Fourteen top cross hybrid genotypes and two checks were used. Highly significant variation (P 0.001) was found among the genotypes for MSV and grain yield. Dak0127, Dak0122, Dak0125 and Dak0124 genotypes were identified as the best materials for yield and tolerant to MSV disease while Dak0123, Dak01211 and Dak01210 genotypes were not good for locations having high MSV disease pressure. Significant and positive correlations between yield and plant height, ear length, hundred seed weight and ears/plant were observed. Path coefficient analysis indicated importance of the same variables through their direct contribution on grain yield. High heritability was observed for grain yield, ear length, number of rows/ear, grains/ear and grains/row, MSV disease incidence and severity, ear height, ears/plant, ear circumference, plant height. Based on correlation analysis earlier silking should be used for increased yield at these locations. High yielding MSV disease tolerant top cross genotypes which were identified are recommended for more seasons’ evaluation for identifying genotypes to replace existing inferior cultivars. 44 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Shemahonge, M. I. MSc. (2013) Improving upland rice (Oryza sativa L.) PERFOMANCE through enhanced soil fertility and water conservation methods at Ukiriguru Mwanza, Tanzania Abstract The experiment was conducted at Agricultural Research Institute (ARI)–Ukiriguru Mwanza, Tanzania from November 2011 to May 2012, aiming at improving upland rice performance through enhanced soil fertility and water conservation methods. A split– split plot experiment in randomized complete block design with three replications and three factors that were (a) upland rice cultivars (WAB 450, NERICA1 and NERICA4) (b) fertilizer types, (urea 80kg N/ha, farm yard manure 5 t/ha and control) (c) three water conservation methods (flat cultivation, open ridge and tie ridge were applied). Rice was sown in seven rows at 30cm inter–and intra–row spacing. Data on soil, weather, crop growth, yield components and grain yield were collected. Rainfall was 651.2mm during the cropping season which was poorly distributed. Average temperature was 24oC with mean relative humidity of 75%. Soil analysis results showed that total nitrogen was 0.08%, phosphorus 2.09 mgP/kg and organic carbon 0.58%. The soil calcium was 3.38cmolc/kg; potassium was 0.36cmolc/ kg, while zinc was 0.39 mg/kg. Cultivars had significant effect on yields which were 2 856, 2 507 and 2 140kg/ha for WAB 450, NERICA4 and NERICA1 respectively. Fertilizer types also significantly affected grain yield in the order of urea (3 368kg/ha) > FYM (2 723kg/ha) and > control (1 421kg/ha). Further, moisture conservation methods significantly affected yield in the order of tie ridges (2 710kg/ha), open ridge (2 398kg/ ha) and flat cultivation (2 394kg/ha). Overall the study results indicated low soil fertility, although it was found to be suitable for upland rice production with further improvement. It is concluded that rice cultivar, WAB 450 had the highest yield potential, while application of urea at 80kg N/ha gave high grain yield, and tie ridges were the best in soil moisture conservation. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 45 Ndee, A. K. MSc. (2013) Introgression of bean anthracnose resistance genes in common bean (PHASEOLUS VULGARIS L.) lines with ALS, CBB and BCMV/ BCMNV diseases resistances Abstract The common bean (Phaseolus vulgaris L) is the most important food legume consumed worldwide. Sustainable production of the common bean in Tanzania is hampered by number of constrains including angular leaf spot (ALS), common bacterial blight (CBB), bean common mosaic virus/bean common mosaic necrosis virus (BCMV/BCMNV) and anthracnose (ANTH) diseases. The objective of this study was to develop common bean lines which will have resistances to ALS, CBB, BCMV/BCMNV and ANTH diseases by incorporating Co42 and Co-6 resistance gene for anthracnose in bean line that has angular leaf spot, common bacterial blight, bean common mosaic virus/bean common mosaic necrotic virus and anthracnose disease resistance. Two donor parents of common bean cultivar C4-1308B-3E-8-B and AB 136 were used as a source of anthracnose resistance and one recipient parent line Vax3 x Mex54 x Mshindi. Crosses for creating generation of segregating population and evaluation for anthracnose disease was done under screen house and field conditions. Significant genetic variation (≤ 0.05) for anthracnose disease was noted. Moreover F1 and F2 plants in all crosses showed significant (≤ 0.05) level of resistance to anthracnose. Thus research work was successful in incorporating anthracnose resistance genes in adapted bean lines and recommended that hybridization by pyramiding genes for disease resistances may therefore be used to improve bean genotype by incorporating the disease traits from donors to a single genotype comprising many diseases in it. It is recommended that the F2 populations created in this study be advanced for further evaluation to ascertain anthracnose, CBB, ALS, BCMV/BCMNV disease resistance, and agronomic data. 46 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mohammed, R. A. MSc. (2013); Agroforestry adoption and its socio-economic contribution to livelihoods in Magu District, Mwanza, Tanzania Abstract Environmental degradation is an issue of concern in Mwanza Region, Tanzania. Deforestation and improper soil management have led to soil erosion results into worsening poverty, food insecurity and low income. Agroforestry practices, soil conservation measure could reduce environmental degradation while increasing farm productivity and income. Promotion and sustainable uses of this option require understanding of the extent of adoption of various agroforestry technologies. This research investigated the socio economic factors that influence the adoption of agroforestry practices and its socio-economic contribution to livelihood in Magu District Mwanza Region. The research design deployed purposive sampling focusing on divisions and wards that have been involved in V-i agroforestry project. Random sampling was used to select 30 household making a total of 117 respondents. Data was analyzed using cross tabulation, Logistics regression model and structural functional analysis techniques. Based on the results 83% of the respondents were engaged in agroforestry practices. Age, gender, marital status, education level and farm size were not found statistically significant to influence adoption. Awareness and land ownership were found positively and statistically significant observed factors influencing the adoption. It was also learnt that agroforestry being the main contributor (31.13%) to household food supply and income compared to business, employment, agriculture and livestock keeping. Adoption of agroforestry practices resulted in better livelihoods for farmers; it is therefore recommended that farmers should continue practicing and adopting agroforestry technologies for better livelihoods, through creation of more awareness among farmers and other stakeholders on environmental degradation problems and strengthening extension services. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 47 Titi, U. F. MSc. (2013) Analysis of demand for private fodder delivery services for enhancing feed resource availability in Meru and Moshi rural Districts Abstract The study on Analysisof demand for private fodder delivery services was carried out in Moshi Rural and Meru Districts in the Northern zone of Tanzania. The purpose of the study was to assess farmers’ demand for private fodder delivery services for enhancing feed resource availability in the study area. Data from 140 households were collected in four villages of Tsuduny and Rau in Moshi rural district and Amburenimwaivaro and Sing’si in Meru district. Purposive and simple random sampling was used to select villages and livestock keepers respectively. Both primary and secondary data were collected. Primary data for this study was collected through informal and formal survey to get in–depth understanding of issue related to fodder availability. Both descriptive and quantitative analytical tools were used to analyze the data. Logit regression model was used to determine the factors influencing the demand for private fodder delivery services. The findings from the model showed that, the demand for private fodder delivery services were influenced by fodder price, farm size and number of cattle kept. It was also found that, 94% of respondents needed the private fodder delivery services. Fodder scarcity was the major challenge faced by farmers in Moshi rural and Meru districts. Fifty four percent of the respondents claimed to face fodder shortage particularly during dry season. This study concludes that the existing actors for fodder delivery do not meet the market demand for the fodder. Thus the study recommends growing different drought tolerant fodder varieties with high nutritive value. However, formulated policies on livestock sector development should be in line with fodder improvement including reserving land for commercial fodder planting. 48 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Raphael, T. M. MSc. (2014) Effects of spacing regimes on growth, yield and wood properties of tectona grandis at Longuza Forest Plantation, Tanzania Abstract This study examined the effects of planting spacing on the growth, yield and wood properties of teak planted at square spacings of 2 m, 3 m and 4 m at Longuza Forest Plantation (LFP), Tanzania. The variables determined were diameter at breast height (Dbh), height (Ht), basal area (BA), survival/stocking, mean annual increment (MAI), total volume production at 1, 2 and 14 years of age. Basic density (BD), heartwood proportion, modulus of rupture (MOR), modulus of elasticity (MOE), compression strength tangential to grain (CS), shear tangential to the grain, and cleavage tangential and radial to the grain were studied in trees aged 14 years only. Growth, yield and wood properties data were collected through tree measurements and destructive sampling. After measuring Ht and Dbh for all trees in each spacing, three trees for each spacing were felled and one-metre long central board was cut at Dbh upward on each tree sample for determination of wood properties. In addition, four disks measuring 5 cm thick were cut at Dbh, 30%, 60% and 90% of total tree Ht for BD and heartwood proportion determination. Dbh and Ht increased while stocking decreased significantly with increasing spacing at older age. MAI increased with spacing although not significantly. At 14 years, spacing did not have significant effect on total volume production and BA while at younger age both decreased with increasing spacing. All studied wood property variables except cleavage tangential to grain were not significantly affected by increasing spacing. It is recommended that if thinning can be conducted earlier before onset of competition at 5 years, the current spacing of 2.5 x 2.5 m be continued as it provides better opportunity of obtaining superior final stand and higher stand volume than other spacings, but if done after five years, then spacing of 3 x 3 m have to be used. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 49 Omar, M. M. MSc. (2013) Developing rainfall erosivity equations for use in Tanzania with limited rainfall intensity data Abstract Soil erosion by water is a growing problem in Tanzania particularly in semi-arid areas and on the mountain steep slopes. The potential for soil erosion is based on many factors. One of the factors limiting erosion control in the country is the availability of rainfall erosivity information. The study focused on developing rainfall erosivity equations for use in Tanzania using limited available rainfall intensity data. The rainfall intensity charts from 13 selected rain gauge stations were used to calculate the daily, monthly and annual rainfall erosivity (El30) values. The calculated annual El30 ranged from 570 (MJ-mm/ha-h-y) to 10 568 (MJ-mm/ha-h-y) which are typical rainfall erosivity values in the tropivcs. The linear, logarithmic and power equations relating the calculated annual rainfall erosivity to annual rainfall volume and Fournier index were developed for each of the 13 selected rain gauge stations. An equation relating rainfall erosivity to annual rainfall volume for all the 13 rainfall gauge stations showed to be the most accurate based on coefficient of determination (R2 ) and the standard error of estimate. The linear equation which had an R2 value of 0.95 and standard error of estimate equal to 505.85, resulting from combined data sets for all stations showed to be the best and was thus recommended to be used for calculating rainfall erosivity in Tanzania. The recommended equationwas used to calculate R-values of 278 rain gauge stations and for developing rainfall erosivity map for Tanzania. The ARC VIEW GIS using Inverse Distance Weighted (IDW) interpolation method was used to develop the Iso- erodent map of Tanzania. The predicted average long term annual rainfall erosivity ranged from about 1 140 MJ-mm/ha-hy to 12 118 MJ-mm/ha-h-y. The highest rainfall erosivity values were for areas receiving highest amount of rainfall volumes including some parts of the Lake Victoria. Uluguru Mountain, part of Northern and Southern Highlands and Zanzibar Islands. The Central semi-arid areas and part of the northern highlands of the country having low annual rainfall volumes have low rainfall erosivity. 50 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mrema, E.J. MSc. (2012) Evaluation of the growth and yield performance of Watermelon varieties during the dry and wet seasons in Morogoro Abstract High yield is a major ambition to watermelon (Citrullus lanatus) plant bleeders and farmers. The purpose of this study was to determine the influence of environmental conditions in Morogoro on the growth and yield of locally available watermelon varieties. A field trial involving six varieties namely Sugarbaby South Africa, Sugarbaby East Africa, crimson Sweet, Patanegra F1, Zuri F1 and Sukari F1, were conducted at the Department of Crop Science and production Crop Museum, Sokoine University of Agriculture between October 2011- February 2012 (dry season) and March 2012 – May 2012 (wet season). The experiment was raid out in a Randomized Complete Block Design with three replications. Data were collected on yield, vegetative and reproductive performance, diseases and pest incidences. They were then subjected to analysis of variance using the GENSTAT13th Edition at a different declared significance of 5% level. Results show significant differences between varieties in yielding components and incidences of pests. Sukari F1 was observed to be the best performing cultivar with good vegetative growth traits which led to high yields. Patanegra F1 was observed to be better in reproductive performance while being low in most of important vegetative growth characters. It was the most infected by the melon fly. Other varieties portrayed average performance in all aspects. However they had various desirable agronomic traits which can be selected in breeding programmes. These include the vegetative and reproductive growth traits and resistance to pest infestation which were found to be highly correlated to yield. It was also observed that cool environmental condition promoted female than male flowers’ formation and thus larger number of fruits were harvested during the wet season. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 51 Joseph, M.C. MSc. (2013) Influence of climate variability on wetland cultivation: A case of Lake Victoria shoreline wetlands in Misungwi District Abstract This study assessed the influence of climate variability on wetland cultivation using a case of Lake Victoria shoreline wetlands in Misungwi District. Specifically the study assessed farmer’s awareness of climate variability taking place in the study area, rainfall and temperature variability and their influence on wetland farming, and lastly assessed wetland cover changes in relation to increased agricultural activities and climate variability. The study used information and data collected from four villages selected from three wards bordering the lake. Primary data were collected by using a semi-structured questionnaire, key informant interviews checklist and observations. Also secondary data were collected from different sources. A total of 147 farmers cultivating in wetlands along Lake Victoria shoreline were interviewed. Furthermore, remotely sensed imageries were used for land use and land cover mapping within the study area. Quantitative socio-economic information wasa analyzed using statistical Package for Social Scientists (SPSS) while satellite images were analyzed using ERDAS Imagine and Arc View GIS software. Mann-Kendal test was used to analyse the trends in rainfall and temperature for different years. Results indicate that rainfall was declining with varying distribution over time. The intermediate rainfall mainly January and February increased slightly while long rains decreased over the whole periods. Maximum and minmum Tem-perature reveal.ed mixed treads (increasing and decreasing) over the different periods under considerations. The land cover/use change analysis revealed that for the period 1975-2011, the cultivated land increased by 15% and open water bodies by 2% while grassland decreased by 11% and closed forest by 9%. The changes were attributable to overgrazing, agricultural expansion, availability of market for horticultural crops and settlements due to population increase as revealed from interviews. Result revealed that 95.9% of respondents interviewed were aware on climate variability. The study concludes that climate variability have had influence on wetland cultivation. 52 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mlay, J. D. M. MSc. (2013) The potential of immunohistochemistry in diagnosis of contagious bovine pleuropneumonia in apparently health animals Abstract Diagnosis of contagious bovine pleuropneumonia (CBPP) in asymptomatic animals is a big challenge towards control of the disease. This is because most, if not all, of the available diagnostic methods are incapable of detecting the disease in such animals. In this study, an immunohistochemistry protocol was developed and employed to detect Mycoplasma mycoides subspcieces mycoides small Colony (MmmSc) type, the casual agent of CBPP< in apparently healthy animals slaughtered at Morogoro urban abbatoir. Lungs (n=13) with CBPP-like lesions were collected from the abbatoir, kept in cool boxes, and transported to the veterinary pathology laboratory for investigation. After thorough gross examination, the lung samples were prepared fo bacteriology, histology and immunohistochemistry using monoclonal antibodies 3H12 and 6E3. Grossly, the the lung were non-collapsing, marbed with widened interlobular septa, oedematous and fibrinous. They were also consoridated and had ares of sequestration. Cultures from two samples showed growth of small colonies with “fried egg” appearance on Mycoplasma Experiencemedium. Histological examination revealed expanded alveoli containing fibrin, oedema and inflammatory cells such as lymphocytes, plasma cells, macrophages and alveolar epithelial cells in all samples. In more severe cases the inflammatory cellsoccupied the lost architecture of the alveoli. There was also fibrinous vasculitis and bronchiolitis with peripheral leukocytic infiltration. In immunohistochemistry, MmmSc antigens were detected from the sections prepared from all collected lung samples in the alveolar lumen and wall, bronchial and bronchiolar lumina, epithelium as well as bronchial glands and cartilage. It is concluded that immunohistochemistry is efficient in detecting MmmSC antigens in apparently healthy slaughtered animals. Its employment could be a sound option in diagnosis of CBPP in carrier asymptomatic animals and could thus aid in a long run, the control of the disease. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 53 Mohammed, S. O. MSc. (2013) Estimates of stability parameters and interrelationship among yield components of orange-fleshed sweetpotato (ipomoea batatas) clones in Zanzibar Abstract Seven sweetpotato genotypes were evaluated in different sweetpotato ecologies of Zanzibar during rainy season ‘Masika’ of 2010. The aim of the experiment was to determine genotypic adaptability, stability and paths of influence exisiting among yield components and the effect of plant spacing of orange-fleshed sweetpotato (OFSP) clones. A randomized complete block design (RCBD) laid in a split-plot design with three replications at three locations was used. The ANOVA revealed that there were insignificant differences between spacing in sweetpotato root yield observed in all three spacings. However significant differences were observed between spacings on vine weight and number of roots where as 20 x 100 cm plant spacing had more number of roots (59) compared to plant spacing of 40 x 100 cm (50). Furthermore there was wide variation across three sites in all traits. With regard to tuber yield, Mayai, SPZ07/188 and SPZ07/951 obtained higher yield at Bambi and Donge sites respectively, whereas SPZ07/188 obtained highest yield across all three sites (13.34 t/ha). In additional to that, average root yield was greater at Bambi (11.8 t ha-1) than at Donge (11.3 t ha-1) or Kizimbani (9.3 t h-1). High heritabilities were recoded for SPW incidence and severity, SPW weighted mean and vine fresh weight viz. 84.4%, 74.8%, 67.8% and 53.5% respectively. Path coefficient analysis showed that vine fresh weight, number of roots per plant and harvest index interacted positively wellin their contribution to the total correlation r = 0.3481**, r = 0347888 and r = 07303** this due to direct influences (0.8037), (0.2043) and (0.6880) respectively on sweetpotato yield while dry matter content showed negative direct effect on the sweetpotato root yield (-0. 4096). Stability analysis revealed that SPZ07/188 was stable on tuber yield performance and therefor recommended for production across all sites. 54 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mbapila, S. J. MSc. (2013) Assessment of pigeon pea export oriented market in Babati and Karatu districts Abstract The object of this study was to carry out an assessment of pigeon pea export market. Specifically to: (i) identify profitability or gross margins of investing inpigeon pea production for export (ii) evaluating the price structure, costs and margins along the pigeon pea value chain (iii) identify constraints facing different actors within the value chain and (iv) identify the key actors in the pigeon pea export value chain. The average gross margin for pigeon pea was 210 860 Tshs for Babati and 484 630 Tshs for Karatu. Costs, prices and margins received by actors in pigeon pea value chain increased downstream. Traders were incurring high cost as moving downstream. This cost resulted into high pricesreceived by consumers and increased in marketing margins downstream. Collectors had the marketing margin of 1.94% for Babati and 4.04% for Karatu. Retailers had the marketing margin of 12.14% for Babati and 28.74% for Karatu. Wholesalers had the marketing marginof 11.21% for Babati and 29.04% for Karatu. Exporters had the marketing margin of 16.70% for Babati and 41.36% for Karatu. Some costs were not trevealed by exporters like Tax/Tanzania Revenue Authority for fear of disclosing their business information. Collectors faced a lot of marketing constraints like price fluctuation, competition with bid companies, low price, and poor quality of pigeon pea, lack of market and lack of marketing information, followed by retailers wholesalers and least marketing constraints tp exporters. Key actors identified in pigeon pea export oriented value chain includes, input suppliers, farmers brokers, collectors, wholesalers, retailers, consumers, exporters and processing industries. Input suppliers for pigeon pea in Babati and Karatu were research institution for experimental purposes, Exporting companies who interred contracts with farmers and stockist. These stockists were also exporting companies like Dodoma transport an exporting company fouind in Arusha and Babati. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 55 Shaali, M. S. MSc (2013) Genetic variability, heritability and interrelationships among yield components of rice (Oryza sativa L.) exotic genotypes in Zanzibar. Abstract The experiments were conducted using 13 rice genotypes from international Rice Research Institute (IRRI) and commonly grown variety (SARO 5) as a check during April-June 2012 growing season at three rice irrigation schemes of Zanzibar. The aim was to estimate genetic variability, heritability and interrelationships among yield components of genotypes so as to identify potential lines for improvement and production. RCBD with three replications at each site was used. ANOVA showed significant differences for all studied characters indicating that genotype constitutes a lot of genetic variability. GCV were lower than PCV in all studied characters indicating considerable influence of environment on expression of the traits. Broad sense heritability was higher for panicle length, number of tillers per plant, plant height, 1000 grains weight, panicle weight and yield per hectare indicating heritable portion of variation; this suggesting that selection can be carried out on the basis of their genotype expression. The correlations that were consistently significant at all locations and in addition to the combined analysis were days to 50% flowering with days to maturity, number of primary branches per panicle with days to 50% flowering, and grain yield per plant with yield / ha suggesting that grain yield per plant should be considered during selection for yield. Path analysis revealed that number of tillers per plant showed positive direct effect on grain yield also had indirect effect via 1000 graoins weight and panicle length . Number of grains per panicle had positive direct effect on grain yield also had indirect effect on yield via number of primary branches per panicle. Panicle length showed positive direct effect on grain yield also had indirect effect through number of primary branches. With respect to yield genotype IRO7M101 was stable and could select for production and further improvement. 56 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mzula, A. MSc. (2013) Molecular epidemiology of Fowlpox virus in Tanzania Abstract The aim of this study was to generate molecular epidemiological information of the Fowlpox virus (FPV) for possible information that may contribute to the development of local vaccine against Fowlpox (FP) in Tanzania. FPV isolation, virulence study, identification, and assessement of REV intergration into FPV genome were conducted using embrynated chicken eggs and Polymerase Chain Reaction (PCR), respectively. A total of 85 fresh scabs from clinically FP infected chickens were collected fro 7 regions in the Country.Fifty percent of the collected samples were identified to be FPV by PCR. Phylogenetic studies showed a close relationshipof the Tanzanian field isolates. Moreever all isolates were calso shown to be clustetred in Clade A and not in Clade B. A minor variation existed in the Tanzanian isolates, where isolates from Dodoma formed independent subclades (A2 an A3) which were attributed to a single nucleotide substitution observed on the aligned sequences. The study revealed high percentage of virulence shown by FPV field isolatescollected from selected regions of the country which may be due to REV integration in the FPV genome. For the first time in Tanzania, detection of REV in FPV genome revealed near full-REV integration in most of field isolates although partial integration was also observed. It is noteworthy that REV free FPV were also identified in the present study. These findings provide a sound basis for further molecular epidemiological and evolutionary studies on FPV in the country. The use of the identification REV free FPV isolates from this study to develop and obtain the best vaccine candidates virus is highly recommended. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 57 Kihwele, L. M. MSc. (2013) The feasibility of scaling up rural institutions for sustainable land management: A case of Pemba Island Abstract This study was conducted in order to find the feasibility of scaling up rural local institutions responsible for sustainable land management in Pemba Island. Specifically the study aimed to identify and characterize the selected local land management organizations, assesss their potentials and constraints in livelihood improvements and land management goals, assess their impacts on sustainable land management and livelihoods, and identify approaches suoitable for up-scaling them. Both primary and secondary data were collected. In collection of primary data, structured questionnaires were administered to 80 farmers drawn randomly from 40 project imp-lementation groups. Also to supplement the responses from structured questionnaire, checklists were used to 40 groups and 40 key informants. Data collected were analyzed quantitatively as well as qualitatively. The results on the characteristics of the local institutions were based on the institutional set up, their strength, type of enterprise, and on the social-economic characteristicsof group members. Farmers’ potentials include knowledge they received during their training, strong institutional capacity, awareness on land degradation problems which occur in their areas and high level of technology adoption (about 83%). Results showed that factors which influenced technology adoption were educational level, type of enterprise, income level of respondents and proximity from village centre. These variables were significant at p<0.01, p<0.05, and p<0.001 respectively. Furthermore, farmers also reported that they are faced with some constraints such as lack of capital, poor market infrastructure and inadequate extension services. The impact of these institutions in sustainable land management and livelihood include; increase in household income, increase in soil fertility and household food security as a result of effective utilization of agricultural technologies and practices. The study concludes that, rural local institutionsfound in Pemba have potential in sustainable land management andneed to be scaled up. The study recommends the scaling up approaches as building capacity on different types of enterprises, financial support, improvement in extension services and networking. 58 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Lukendakenda, P. P. MSc. (2013) Factors influencing sustainability of diary farmer groups in the Muheza District of Tanga Region, Tanzania Abstract The need to archive sustainable dairy groups I an ideal in the process of raising farmers’ income in the diary industry. Based on this ground, the study was conducted in two divisions of Muheza West and Amani East in Muheza District to assess factors influencing susytainability of diary farmer groups. The study objective were (i) to identifify the nature of available dairy farmer groups, (ii) to assess perceptionof group and ex- group members towards dairy farmr groups, and (iii) to identify factors influencing the sustainability of dairy farmer groups. A total of 120 respondents of 60 group and 60 ex-group members were randomly selected and interviewed. Descriptive statistics such as frequency and percentages were calculated to determine distribution of the study variables, while the Chi- square tested the significance different between the independent and dependent variables. The results showed that the un-sustainability of the dairy farmer group is caused by various factors including poor group leadership; low frequency of group meetings, poor record keeping; lack of work plan; low milk price; poor internal resource mobilization and high maintemance cost. This implies that dairy farmer groups will continue to be un- sustainable unless these factors are well addressed. Furthermore the study reveal that both group and ex-group members had negative perceptions toward dairy farmers groups due to inadequate fundamental components of sustainable group such as group stability, group success, group performance, and members‘ benefits, and this contribute to their withdrawal. From the findings of this study, recommendations are made to the change agents and group leaders to facilitate and provide supportive supervision of the groups so as to ensure stability in groups, group success, good performance, svailability of stable governing policies and accruing benefits in sustainable manner through strong, active, and committed leaders who are capable of leading the groups to meet the set objectives. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 59 Nzilano, B. L. MSc. (2013) Contribution of Agroforestry homegardens to household food security and income generation among communities in Mbeya Rural District, Tanzania Abstract The percent study was conducted in Mbeya Rural District during December 2011 to April 2012 with the main objective of assessing the contribution of Agroforestry homegardens to household food security and income generation among communities. The study involvedfour randomly selected wards, one village per selected ward and thirty households from each village, all selected randomly. Data collection was done through field surveys using relevant social survey methods. The findings indicated that 9% of the communities in Mbeya Rural District practice Agroforestry Homegardes as one of their main sources of household food security and income generation. Six sources of household food security and income generation were identified in the study area with Agroforestry homegardens and agriculture being the main source of life sustenance. Of the Agroforestry Homegardens contributions, seventeen percent (17%) is towards household food security and twenty five percent (25%) towards income generation. Accorging to the communities studied, food security and income generation over the year from 78% of the motivating factors to adopt the Agroforestry homegardens. Crop competition, low capital and unreliable markets, were together the main factors (by 77%) which negatively affected the contribution of Agroforestry homegardens to household food security and income generation in the area. Sixty seven percent (67%) of the communities indicated that credit facilities, knowledge on components arrangements and market availability for homegardens products, were the main interventions required in improving the contribution of Agroforestry homegardensadoption. Based on these findings, Agroforestry homegardens is a major source of food and income generation in Mbeya Rurakl District. Therefore, there is a need for enhancing and reviving further the Agroforestry homegardens technologies for the benefits of the wider communities beyond the District. This should go hand in hand with the provision of sufficient credit facilities, extension services together with marketing arrangements. 60 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Saidia, P. S. MSc. (2013) Response of upland rice to activated Effective Microorganisms (EMA), farmyard manure and nitrogen at Ukiriguru Mwanza, Tanzania Abstract A field experiment was conducted too investigate the response of upland rice to nitrogen as urea, farmyard manure and activated effective microorganisms (EMA). Low yields of rice due to low soil fertility status and soil moisture stress are prevalent in all rice growing area of Tanzania thus causing food insecurity and low income generation. The overall objective was to increase rice yields through the adoption of integrated soily fertility management. Characterization of soils for fertility status, farmyard manure and effective microorganisms was undertaken. A three factor factorial experiment in a randomized complete block design was used in the field, treatments were urea split applied at 0, 50 and 1000 kg N ha-1 at emergency, tillering and panicle formation stages, farmyard manure at rates 0, 5 and 10 10 t ha-1 applied two weeks before sowing by broadcasting method and incorporated into soil and EMA at rates 0, 20 and 40 L ha-1 at dilution rate 1:500 in water applied once per week throughout the vegetative growth stage. Application of N as urea increased rice yields by as much as 2.48 t ha-1; farmyard manure 1.97 tha-1 and EMA 1.34 tha-1. High yields were obtained for the urea–farmyard manure-EMA treatment combinations ranging between 4 and 5 t ha-1. It was concluded that the soil fertility status at the study area was low and the major soil fertility limitations included low moisture and nutrient retention capacity, low nitrogen, low exchangeable cations, and low plant available copper and zinc. Organic materials used were of medium quality and EMA solution contains a variety of the essential plant nutrients. It is recommended to adopt integrated approaches in soil fertility and nutrient management for sustainable rice production. Also more research should be done on EM solutions as source of plant nutrients. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 61 Kachiwile, A. Z. MSC. (2014) Drought tolerance assessment of cassava genotypes in a semi-arid environment in Central Tanzania Abstract Dorought tolerance is an increasingly important trait in cassava due to globally dwindling water resources, a shift in production area and increasing input costs. This study was conducted to evaluate eighteen cassava genotypes for drought tolerance to counteract frequenct shortage of rainfall. An experiment was set in Dodoma, Tanzania which is a drought –stricken environment where cassava genotypes were grown under watered and water stressed conditions. Identification of drought toleranct genotypes that also had good yield potential was facilitated by the stress treatments used on farm. Morphological (leaf length, leaf width, plant height), physiological (leaf retention, chlorophyll content), biochemical (Catalase and Peroxidase enzymes activities) and yield (number of roots per plant, yield in tons per hectare, above ground biomass, percentage dry matter content and harvest index) attributes were used to assesss tolerance of the tested genotypes to drought stress. During harvesting, on farm farmers participatory evaluation and organoleptic test were also conducted to enable farmers select best genotypes based on consumers criteria. It was found during the study that water stress had profound effect on growth, physiochemical and yield performanceof cassava genotypes. Allattributes measured were significantly influenced by drought except leaf length at 180 DAP, plant height at 120 and 150 DAP, number of roots per plant, weight of above ground biomass and harvest index (P≤0.05). Generally water stress suppressed both growth and yield of the crop. Through the study, superior drought promising cassava genotypes identified were 92B/00073, KBH 2006/363, KBH 2006/12 and KBH 2006/18. Tthese genotypes performed well under stress and well watered conditions and thus may serve as parents for drought stress improvement and genetic analysis. 62 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Tewele, C. P. MSc. (2012) Susceptibility to Sitophilus zeamains and nutrient content of traditionally stored maize: a case study of Mikese (Morogoro Rural District) Abstract Maize (Zea may) is the most important cereal crop grown and consumed in Tanzania. It is a staple food in many countries in Africa. However, it is susceptible to pests while in storage. The study aimed at evaluating susceptiblility to storage pests and nutrient content of common maize varieties stored using traditional methods. Household survey on cultivated maize varieties and traditional, methods used to store maize was conducted in Mikese. Laboratory experiment was set up for six months to determine extent of damage by insects and proximate composition of tested maize varieties. Comparison of the parameters in extent of damage and proximate composition were done using ANOVA. Control samples were observed to have significantly (p<0.05) higher number of damaged grain than other treatments. The highest (19.5%) grain damage occurred in control and the lowest in wood ash (6.4%). Grain damage was dignificantly (p<0.05) higher in TAN250 followed by Staha and stuka M1 nbeing the least. After the second and fourth months of storage, the composition of the grains differed significantly (p<0.05) in ash, CP, EE, CF and CHO except for the grains that were protected by actellic super dust,. Stuka M1 was more resistant than Staha and TAN 250 for all treatments and wood observed to be more effectivethan rice husksand unminnowed storage. Stuka M1 maize variety could be utilized as an environmentally friendly way to reduce damage by S. zeamais under traditional storage conditions. It is recommended that wood ash, rice husks and unminnowed storage are disseminated to areas with pest restant maize varieties. This will assist small scale farmers whose majority cannot afford synthetic pesticides to store their produce thereby improving food security and ensuring food safety. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 63 Ngalla, R. J. MSc. (2013) Factors contributing to food insecurity during crop growing season in Handeni District, Tanga Region Abstract Food insecurity is widespread and chronic that there is always a certain degree of household food deficit at a given part in a year. This study therefore aimed to assess factors contributing to food insecurityduring crop growing season in Handeni district, Tanga Region. Specific objective were to determine food insecure households, to ascertain the socioeconomic aspects perpetuating food insecurity, to describe the exisiting institutional arramngement on alleviating food insecurity and to identify the coping strategies employed at household level to lessen food insecurity. Data was collected from four villages of Muungano B, Kwaluala, Tuliani and Kwankonje. Sample of 132 respondents was used, that include head of households, village Agricultural Extension Officers and village leaders. Frequencies, percentages, Chisquare and multiple linear regressions were employed to analyse data. The result indicates that majority of the respondents in the study area were food insecure. Very few were food secure. Results revealed further that socio-economic factors influencing household food security were numbers of people working, farm size, land used for farming activities, and annual income.Major coping strategies adopted by respondents in the study area to lessen food insecurity were skipping meals, selling labour, and get support from the government and NGOs. It was found that world vision and the government was major institutions participating on alleviating food insecurity in Handeni District. In conclusion despite the food insecurity coping strategies, a serious food insecurity problem still persist. The study recommends that the government should strive to protect local market to increase household annual income, farmers should organize themselves to form groups or SACCOS which will enable them to access credit from lending institutions and also groups may arganize training programmes on entrepreneurship skills and invite trainers or facilitators to train the members on entrepreneurship to raise their income. 64 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mpore, F.J.MSc. (2013) Nutrient content, Microbiological quality and sensory acceptability of sun and solar dried raisins from Dodoma Region Abstract Grape (Vitis venifera) is a horticultural crop which covers more than16% of the fruit production in the world. However, are highly perishable. The study aimed at assessing nutritional quality, microbiological quality and sensory acceptability of raisins produced using open sun drying, cabinet solar drying and tunnel solar drying from four varieties of grapes ruby seedless, beauty seedless, chenin blanc and Makutupora white. Nutritional quality which included proximate analysis, vitamin C and mineral content was determined using AOAC methods. Sensort attributes such as colour, taste, aroma, texture, moth feel, consistency and overall acceptability of raisins was assessed Microbial numbers were determine by planting using nutrient agar for total aerobic mesophilic bacteria count, most probable number (MPN) using lawry sulphate tryptone broth for coliforms and Rose Bengal chloromophonical agar for yeasts and moulds. Comparisons of parameters in nutrient composition and microbial count were done using ANOVA. Vitamin C was higher in Makutupora white raisins variety dried using tunnel solar drier (8.89mg/100g) than those dried in open sun drier (1.8mg/100g) and cabinet solar drier (6.05mg/100g). Proximate composition showed a significant differences (p<0.05) between varieties and within drying methods. The three drying methods showed no effect in raisins mineral content. Mineral content was significantly different (p<0.05) among the four raisins varieties. Iron, calcium and Sodium were in highest concentration in beauty seedless variety. Microbial analysis revealed no growth of aerobic mesophilic bacteria, coliforms and fecal coliforms in solar dried raisins. The open sun dried sample showed yeasts and moulds growth. Sensory evaluation showed significant difference (p<0.05 in preference among the four raisin varieties. The study recommended tunnel solar drier for raisin production as is the fastest and maintains most of the nutrients. This will assist small scale farmers to add valu and increase shelf life of their grapes. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 65 Salehe, F. S. PhD. (2014) Irrigated Agriculture and Schistosomiasis in Tanzania: A case of Morogoro and Kilimanjaro Regions Abstract Although there is significant information on schistosomiasis in Tanzania, most of studies considered a single irrigation scheme in a particular geographical location and also available information on schostosomiasis prevalence and knowledge is based towards school children. Therefore this research was done to determine the linkage between irrigated agriculture and schistosomiasis in modern, improved traditional and traditional schemes in Morogoro and Kilimanjaro Regions, Tanzania. Specifically the research itended to: determine socio-economic effects of schistosomiasis among paddy irrigators in the two Regions; assess farmers’ knowledge on schistosomiasis cause, effects, symptoms, transmission and control; and assess schistosomiasis occurance and identify risk factors existing in the stiuy areas. About 858 farmers were chosen for the study and data were collected using a structured questionnaire guided with closed and open-ended questions, focus group discussions, key informants interviews and field observations. T-test was used to determine variation in rice production, amount of money and time spent on schistosomiasis treatment and care and net working hours per month for infected farmers only between schemes, and between infected and non-infected farmers between schemes with the similar infrastructure between the two Regions. One way ANOVA was used to test between sheme defferences for infected farmers and chi-square test was also used to compare proportions of schistosomiasis knowledge of irrigators between shemes with similar infrastructures between the two Regions. Chabi and lower Moshi schemes had larger number of infections compared to other schemes. Moreover, there were no significant differences on income between infected versus non infected formers within schemes in the Region. Schistosomiasis occurrence was high in MKindo while Njoro had the least. High schistosomiasis coccurence in Mkindo may be associated with poor management of drainage systems. 66 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Farmers’ knowledge on schistosomiasis was higher in Kilimanjaro schemes than in Morogoro schemes. The study supported resilience theory since income balance between schistosomiasis infected farmers versus those not infected remained stable within schemes in spite of schistosomiasis burden. Improving farmers’ knowledge on schistosomiasis in all schemes through awareness creation would reduce the risk of infection. A combination of schistosomiasis control measures should be used in minimizing schistosomiasis transmission. Jeckoniah, J.N. PhD. (2013) Value chain development and women empowerment in Tanzania: a case of onion value chain in Northern Tanzania Abstract Women play critical roles in agricultural value chains in developing countries. However, their participation in value chain development activities does not always result into their empowerment. The main objective of this study was to examine the impacts of women participation in onion value chain development activities on their empowerment. The study was conducted in Simanjiro District and adopted a cross-sectional research design. Qualitative and quantitative data were collected using focus group discussions, key informant interviews and household surveys whereby 402 women of whom 207 (51.2%) were participants in the onion value chain development activities and 195 (48.5%) were non-participants. Moreover, eight focus group discussions were conducted. Qualitative data were analyzed using ethnographic content analysis technique with constant comparison; quantitative data were analyzed using the Statistical Package for Social Sciences. Descriptive statistics were computed to establish the profiles of research participants; gross margin were calculated to establish profit margins of different chain actors; Cumulative Empowerment Index was constructed to gauge the overall women empowerment. Principle Component Analysis was used to compute wealth quintiles as proxy for household socio-economic status. F-test was used to compare the levels of empowerment by socio-economic status. Ordinal logistic regression model was used to establish the determinants of women empowerment in onion value chain. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 67 Changes in gender roles, especially in production, were found. Women’s perception on their empowerment was linked to different achievements in their lives in their social and economic contexts and was categorized into medium level of empowerment (CEI = 0.6033). Women participation in decision making was higher and farmer groups were an important source of knowledge for change. The relationship between socio-economic status and women empowerment was statistically significant (P < 0.05). Ordinal logistic regression analysis revealed the predictors of women empowerment in the chain to be: marital status, education level, age at first marriage, land ownership, access to credit and participation in onion value chain (P < 0.05). It is recommended to the government and non-governmental organisations to promote men’s involvement in women empowerment efforts, to improve women access to resources and strengthen farmer’s organisations to competitively participate and get benefits from onion value chain. 68 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS MUHIMBILI UNIVERSITY OF HEALTH AND ALLIED SCIENCE (MUHAS) Rweyemamu, I. K. MSc. (2013) Knowledge, Sexual behaviors, Pregnancy experiences and HIV prevention among adolescents living with HIV from early childhood in Dar es Salaam, Tanzania Abstract Due to the widespread use of antiretroviral therapy, most children who have been living with HIV/AIDS from a young age are healthy and reach their adolescence and early adulthood. While most of these adolescents have started exploring their sexuality, their preventive practices are reported to be inadequate thus increasing the risk for onwards transmission of the infection. This study aimed at assessing knowledge, sexual behaviours, pregnancy experiences and HIV preventive practices of adolescents who have been living with HIV from early childhood. The results that have been obtained will be channeled to programmes providing care to these adolescents in order to institute appropriate interventions. A cross sectional study was carried out at five Care and Treatment Centers (CTC) led by the Management and Development for Health (MDH) programme for a period of 9 months. The study population included adolescents of 10-19 years of age who have been living with HIV from early childhood. The study used both quantitative and qualitative methods. Quantitative data was collected using structured questionnaires while qualitative data was gathered through in-depth interviews. Analysis of quantitative data was done using NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 69 SPSS while that for qualitative data was done through content analysis. The study enrolled 300 adolescents. Over 80% of them could correctly identify the means through which HIV can be transmitted and prevented. Thirty nine percent of these adolescents had penetrative sex and 22% reported to have had unprotected sex at least once. The only significant risk factor for unprotected sex was having a lost mother. About 10% of the female adolescents reported to have ever been pregnant with about a third of these having living children. The main findings from the qualitative data were that the poor preventive practices were a consequence of fear of disclosure of HIV status to sexual partners, fear of rejection by the partners and sense of powerless shown by the women in negotiating safer sex. It is concluded that adolescents who have been living with HIV from early childhood have good knowledge on HIV transmission and prevention. Penetrative sex among them is common however their preventive practices are poor thus posing a risk for secondary transmission of HIV. There is therefore, a need for programmes dealing with HIV infected adolescents to concentrate their care on the issues regarding sexual and reproductive health especially on promotion of safer sex, disclosure of HIV status to sexual partners and women empowerment in order to prevent secondary transmission of HIV/AIDS. 70 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Lusekelo, M. MSc. (2013) Assessment of cardiovascular risk factors and level of malnutrition among the elderly of rural and urban areas in Morogoro, Tanzania Abstract Background: The rise in elderly population globally, is apparently accompanied by the increase in prevalence of Cardiovascular Risk Factors (CRF) and nutritional challenges. Methodology: A descriptive cross-sectional study was conducted to determine the levels of commonCRF and nutritional status among the elderly of urban and rural areas in Morogoro region. A sample of 300 elderly participants was studied and the Body Mass Index (BMI) (kg/ m2), lipids profile (mg/dL), Fasting Blood Glucose (FBG) (Mmo1/L), Blood pressure (BP) (mmHg) and mini nutritio score points were determined. Results: Of the 300 participants, (73.4%) had dyslipidemia and prevalence was higher in female sex (P<0.001).The prevalence of hypertension was 46.3% and was higher in female sex (P<0.05). Dyslipidemia and hypertension were the most prevalent and were more revealed among the urban residents. Obesity, more diagnosed in females sex was revealed in (27.3%) of 300 participants and cases were highly found among the urban occupants. Hyperglycemia marginally higher in female’s sex was found in 19% of 300 participants and the number was marginally higher in the urban compared to rural dwellers. Association of hypertension with obesity (P<0.01, RR=1.5), hyperglycemia (P<0.05, RR=1.5) and dyslipidemia (P<0.05, RR =1.7) was revealed statistically and may elderly with obesity were also diagnosed with dyslipidemia (P<0.05, RR=2) and hyperglycaemia (P<0.05, RR= 1.8) respectively. Association of smoking with lowered High Density Lipoprotein (HDL) was also revealed in the stiudy (P0.05, RR =1.6). In this study were also revealed some cases of nutritional problems as it was observed that 24.6%, (n=300) of the studied elderly, were either malnourished or at risk of malnutrition was more revealed among the rural residents when compared to the urban living elderly people. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 71 Conclusion: The results revealed a burden of CRF and nutrition challenges among the ≥60 aged elderly people of Morogoro in Tanzania. Creating awareness on healthy life styles may help to ameliorate the modifiable CRF and nutrition challenges hence perpetuate the healthy elderly population. Keywords: Prevalence, cardiovascular risk factors, Malnutrition, Elderly Philiph, J. MSc. (2012) Bullying and associated psychosocial factors among secondary school adolescents in Dodoma municipality Abstact Background: Bullying is a global problem and it is an area that is widely researched today, primarily because of its potential impact in a variety of areas like, ongoing antisocial, criminal behaviours, substance abuse, and poor academic achievement.. Bullying negatively impact the health and development of both victims and bullies. This study aimed to begin to address this problem. Objective: To determine the prevalence of bullying behaviors and associated psychosocial and demographic factors among secondary school adolescent in Dodoma Municipality. Design: The study was descriptive cross-sectional study design; community based used quantitative data collection method. Setting: Nine secondary schools in Dodoma municipality where adolescents in form one to form three had participated in the study. The school type was day coeducation, boarding coeducation, boys and girls only boarding secondary school. This study took place between October 2011 and April 2012. Participants: A total of 350 participants responded in this study (men age, 16.5 years). Methods: A self administered PRQ Tool modified by a researcher was distributed to 350 eligible adolescents in nine secondary schools. Data analysis was done by SPSS version 16 using bi-variate and multivariate statistical analysis with significant level set at p<0.005. 72 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Results: This study found a prevalence rate of 36%. Male adolescents were more likely than girls to be bullies and victims of bullying OR 0.23, p-value 0.001. Schools types were also more likely to influence bullying OR 1.5, p-value 0.04. Psychosocial factors and being a bully or victim was significantly statistically associated with bullying OR 3.21, p-value o.003. Conclusions: Gender, school type and parenting style seem to be associated with bullying behaviours among adolescents in secondary schools. Therefore, as adolescents with bullying behaviours are increasing, schools should be encouraged to undertake preventive measures to avoid bullying behaviours. Parenting programs should be initiated in order to create awareness and preventive strategies. Kenyunko, K. M. MSc. (2013) Profile of trochanteric fractures at MOI: treatment and short-term outcome 2012 Abstract Tronchanteric fractures are one of the hip fractures, common in old age. However, they are not uncommon in younger age group. They commonly occurs in elderly following low energy trauma. In young people, these fractures result from high energy trauma. Despite different treatment modalities of these fractures, complications do occur. Study objective: This study aimed to determine the cause of injury, treatment modalities used and short-term outcome of trochanteric fractures at Muhimbili Orthopaedic Institutes (MOI) from January 2012 – December 2012. Methods: A cohort study was done on adult patients with tronchanteric fractures admitted and treated at Muhimbili Orthopaedic Institutes (MOI). Ninety one patients met the study inclusion criteria, four were lost follow-up, and hence 87 patients were reviewed and completed the 16 weeks of follow ups. The structured questionnaires were used to interview the participants. These patients were treated either surgically or non surgical. Those who were surgically treated, immediate post-operative radiographs were taken, length of the lower limb and coxa vara deformity were assessed. Patients who were treated none surgically were put on skin traction for four weeks, then control radiographs were taken, lower limb length and coxa vara deformity were again assessed. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 73 There after each patient was followed up for a period of 16 weeks post treatment. In the follow-up visits, state of the wound, limb length discrepancy, coxa vara deformity and function of the hip joint were assessed. The obtained data were then analyzed by using SPSS version 16.0 Results: In this study 49% of cases occurred after simple fall, 43.7% of cases occurred after Road Traffic Crash (TRC). Average age of occurrence was 62.26 ± 19.19 years with male to female ratio of 2.1. Most cases were Evan’s type II classification. 60 patients were surgically treated and 27 patients were treated conservatively. Of the participants, 37.9% had coxa vara, 40.2% had limb shortening 85.1% had score of above 20 by Salvati – Wilson hip score Conclusion: Trochanteric fractures occur due to simple fall and road traffic crash. Most of these fractures have better outcome when surgically treated. Lucas, H. MSc. (2013) Prevalence and pattern of cardiac disorders among pregnant women attending antenatal clinic at their first clinic visit at Muhimbili National Hospital Abstract Background: Increased cardiac demands during the course of pregnancy potentially increased morbidity and mortality in women with underlying heart diseases. In our setting, most women in child bearing age become pregnant without appraising their health particularly cardiac status and no effort are made at antenatal clinic to screen for cardiac diseases. Improved quality of health care at delivery facilities in some countries (including Tanzania ) has lead to improved survival rates for those with congenital and valvular heart disease leading to an increasing number of heart disease patients who are reaching child bearing age, problems in most young women remain un detected especially in those with minor cardiac disorders. It is consequently essential to thoroughly evaluate expectant women for underlying cardiovascular disease in order to provide optimal care during pregnancy. Objective: To determine the prevalence and pattern of cardiac disorders among pregnant women attending Antenatal clinic at Muhimbili National Hospital (MNH) – Dar es Salaam. 74 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Methods: a hospital based descriptive cross-sectional study done from june to December 2012. A total of 310 pregnant women attending their first antenatal clinic at Muhimbili National Hospital (MNH) were recruited using a systematic sampling technique. A standard structured questionnaire was used to collect demographic data and other cardiac related symptoms. Each participant also had a thorough cardiovascular examination; Electrocardiogram and Echocardiogram were performed to identify types of cardiac disorders. Results: The overall prevalence of cardiac disorders among pregnant women attending antenantal clinic was found to be 29.7%. The prevalence of cardiac disorder (detected by ECHO) was found to be 20.6%. Of these pregnant women 30 (9.7%) had LVH likely due to chronic hypertension, followed by Mitral valve prolapsed in 25(8.1%) pregnant women. The most frequent presenting symptoms and signs were easy fatigability in 221 (71.3%) followed by edema of the lower limbs in 190(63.1%) and palpitations 169 (54.9%). Majority of clinical findings were not significantly associated with the presence of ECHO abnormality. Conclusion: The prevalence of cardiac disorders among pregnant women attending their first ANC at Muhimbili National Hospital was to found to be high at 29.7%. hypertension was found to be the most prevalent cardiac related disorder followed Mitral valve prolapsed however the prevalence of congenital heart disease was found to be low and majority of pregnant women have not been screened before regarding their cardiac status. Recommendations: Screening for cardiac disease as part of ANC should be done and preferably women should be screened for cardiac disorders before pregnancy. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 75 George, U. MSc. (2013) Components of systemic inflammatory response as predictors of mortality in children presenting with fever at the emergency medical department, Muhimbili National Hospital Abstract Introduction: Sepsis is defined as a known or suspected infection in a patient with elements of the systematic inflammatory response syndrome (SIRS). Septic patients present with a variety of clinical manifestations, but temperature dysregulation, tachycardia, tachypnea, and abnormal white blood cell (WBC) count are considered cardinal components of SIRS. We investigated the predictive value of SIRS criteria for in-hospital mortality among febrile children under 5 years old presenting to the Emergency Department (ED) at Muhimbili National Hospital in Dar es Salaam, Tanzania. Methods: This was a descriptive cohort study of febrile children under 5 years, presenting to our ED. Providers prospectively completed a standardized data sheet. Outcome data was obtained from hospital records and telephone follow-up. Study data were entered into Excel (Microsoft, Redmond, WA, USA) and analyzed in SAS 9.3 (Cary, North Carolina, USA0. Results: We enrolled 105 patients between August and November 2012. The median age was 14 months, with 80% over 6 months old, and 63.8% were male. 57 (54.3%) children were referred from outside facilities. The overall mortality rate was 19%, and 90% of children who died had ≥2 SIRS criteria. Mortality in children with ≥2 SIRS criteria (in addition to fever) was significantly higher (27.7% versus 5% than in those with 0-1 SIRS criteria, and children with fever and > 2 SIRS criteria were seven times more likely to die (OR 7.05, p=0.01). 85 children were discharged from the hospital, and of the 64 (75.3%) children we were able to reach after discharge, all were alive 14 day telephone follow-up. 19/85 children who survived to hospital discharge were lost to follow up. Conclusion: SIRS criteria may be helpful to predict febrile children at high risk of mortality. Further studies are needed to validate these findings in larger cohorts. 76 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Charles, L. L.MSc. (2013) Factors influencing sustained transmission of lymphatic filariasis in school children after eight rounds of mass drug administration: A case of mafuriko village in north-eastern Tanzania Abstract Background: The program to eliminate lymphatic filariasis in Tanzania in Tanzania is based on Mass Drug Administration (MDA) with ivermectin and albendazole once per year for 4-6 years in order to interrupt transmission. One of the indicators used to measure the impact of MDA is the prevalence of circulating filarial antigen in children born within the intervention period. It is thought that at a good coverage of ≥ 80% and more than 4 rounds of MDA these children are not expected to have infection (WHO, 2005). High prevalence of circulating filarial antigen of 6.4% has been reported in Tanga despite 8 rounds of MDA in the region (Simonsen et al 2011). This suggests that there is an ongoing transmission of lymphatic filariasis in Tanga. Objective: This study was designed to determine factors influencing sustained transmission of lymphatic filariasis in Tanga based on the findings of high prevalence of circulating filarial antigen in children born within the elimination program. Methodology: a descriptive cross-sectional study involving individuals aged 6 years and above was conducted at Mafuriko village along the coast of Tanga. The study had three parts, an entomological component that include collection and determination of infection and infectivity rates in vectors, parasitological component that involved night blood examination for microfilaria detection and quantitative studies to assess community knowledge and awareness of the LF elimination program, to determine the proportion of individuals who took drugs during the last round of MDA and to assess the rate of insecticide treated bed net utilization. The study recruited 317 study participants. Results: The microfilaria prevalence among the study participants was 6%. The prevalence was high among males (4.7%) than females (1.3%). The most affected age group was the youth below 26 years of age with the prevalence of 19.2% p=0.09. History of ever participated in MDA was found to be protective [AOR=4.6, 95%CI (1.6-13.9)], only two mosquitoes out of 555 were found to harbour LI and L2 and all collected vectors were Culex quinquefasciatus. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 77 Ninety one percent (91%) of study participants were aware of lymphatic filariasis (LF) and 79% were aware of the LF elimination program, 99.3% had good knowledge about LF elimination program and 54% swallowed the drug in the last cycle of treatment. 76.7% participants reported to possess an ITN, among these 93.4% used the ITN the night before the interview. Conclusion: Presence of infected individuals, infected mosquitoes, poor compliance to treatment, low levels of knowledge on LF elimination program, transmission and prevention are the main factors that may influence high prevalence of CFA in school children at Mafuriko village. Longopa, G. MSc. (2013) Pattern of headache among patients attending at Muhimbili National Hospital neurology clinic-2012 Abstract Introduction: Headache or cephalalgia is pain anywhere in the region of the head or neck. It can be a symptom of a number of different conditions of the head or neck. Headache disorders are among the most common disorders of the nervous system which is associated with personal and societal burdens of pain, disability, damaged quality of life and financial cost. It is one of the most common types of recurrent pain as well as one of the most frequent symptoms in neurology. Similar study was done in the same clinic more than twenty years ago, however a previous study described the pattern of headache using HIS criteria and there is no new study that is known to use the new diagnostic criteria since then. Also with recent advancements in technology together with the development of disease like HIV/AIDS lead to the development of new etiology of headache and epidemiological transition of diseases. Objective: To establish the pattern of headache among patients in Neurology clinic at Muhimbili National Hospital (MNH). This was a Hospital based descriptive cross sectional study among outpatients at neurology clinic at MHN. A total of 278 patients with headache were consecutively recruited from May to December 2012. A structured questionnaire was used to collect data on demographics and symptoms of headache. International classification for 78 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS headache disorder volume 11 (ICHD)-11) was used to categorize different types of headaches. Data was entered and analyzed by using SPSS version 10. Chi-square test was used to determine the association between the different predisposing factors and different types of headache. Student test was used to compare means of two variables. P-value of <0.05 or less was taken as significant. Results: During the 8 months period, a total of 1440 patients attended the MNH Neurology clinic. Of these 278/1440 (19.31%) had recurrent Headache. Females were 179/278 (64.38%). Primary headache was seen in 151/278 (54%) patients, of these 103/151 (37%) suffered migraine. No significant difference in gender among migraine, Cluster headache (CH) and Tensional type headache (TTH) patients. Patient aged 60 years and above who were attended suffered from Migraine were 7/11 (63.3%). Anxiety 40 (37.7%, Smoking cigarette 9 (37.5%) and Depression 25 (30.1%) were the major precipitants of Migraine type headache, Tensional type headache was associated with cigarette smoking (33.3%) and Oral contraceptive use 25.0%. Thirty nine patients came at neurology clinic without the referral diagnosis of headache of which 24 (61.5%) of the patient were classified as having migraine headache. Conclusions and Recommendations The pattern headache was dominated by primary headache, similar to the findings of the study done 20 years ago at the same clinic. Primary headache especially migraine was common in patients 60 years of age or older. Primary headache types were highly associated with anxiety and depression. The referral bias observed in most of the results findings necessitates the need for further studies to evaluate similar pattern in a non-specialized hospital. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 79 Hillary, E. MSc. (2013) Assessment of knowledge and skills of cardiopulmonary resuscitation among nurses working in critical care units at Muhimbili National Hospital, Dar es Salaam Tanzania Abstract Background: Nurses who work in critical care units are expected to have high knowledge of current CPR guidelines in order to provide effective Caldiopulmonary Resuscitation (CPR) to Cardiac arrest victims. With documented deterioration of knowledge and skills post CPR training among health care providers and the updating of CPR International guidelines every five years, it is recommended to conduct frequent assessment and when needed refresher training should be done in order to maintain CPR knowledge and skills among nurses and ensure provision of effective CPR. Objective: The objective of this study was to assess knowledge and skills regarding current 2010 Basic Life Support American Heart Association (AHA) CPR guidelines among nurses working in critical care units at Muhimbili National Hospital, Dar es Salaam, Tanzania. Materials and Methods: The researcher utilized quantitative, cross-sectional and observational designs for this study. Seventy three nurses were conveniently sampled out of 104 who met the inclusion criteria. Data was collected using structured questionnaires for CPR theoretical knowledge. For practical skills assessment, the participants were requested to demonstrate their single rescuer CPR Basic Life Support (BLS) skills on a non electronic manikin while a checklist developed by the researcher was used to assess the practical skills. The knowledge and skills of nurses was assessed according to the current 2010 AHA CPR guidelines. ata was analyzed using SPSS Version 17.0. Results were summarized using frequencies, mean and percentages. Knowledge and skills levels were established using percentage scores. Results: More than half (59%) of participants had received CPR training, of which 76% received Basic Life Support CPR training. Majority of the participants 86% scored less than 50% on theoretical knowledge questions with the overall mean score of 32%. On practical skills 84% of participants scored more than 50% with an overall mean score of 62%. There was no association between CPR training and level of CPR theoretical knowledge among participants (p value=0.51). However the high level of CPR practical skills knowledge among participants was associated with CPR training (p value < 0.0001). 80 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Approximately 60% of nurses scored less than 50% on knowledge on COPR sequence of events, rate of chest compression, shockable rhythm recognition and defibrillation components of CPR in both theoretical and practical skills CPR knowledge. More than 40% of nurses in critical care units at Muhimbili National Hospital have not received CPR training. The level of current 2010 AHA CPR guideline theoretical knowledge was below average in majority of the participants. However they demonstrated above average CPR practical skills. CPR training could not have an effect on level of CPR theoretical knowledge but it had a positive effect on CPR practical skills. Majority of participants demonstrated extreme knowledge deficiencies on key components of CPR in both theoretical and practical skills knowledge. Recommendations: Efforts should be made to establish resuscitation councils in Tanzania. More studies need to be conducted to examine the methodology and contents used in theoretical CPR trainings at MNH and to assess the effectiveness of high CPR skills observed in this study. More formal CPR training regarding the new guidelines should be conducted at MNH and future trainings should address the specific knowledge deficiencies observed in this study. Msolla, R. MSc. (2013) Factors associated with late reporting for health care among patients having oral maxillofacial tumours or tumour-line lesions attending Muhimbili National Hospital Abstract Introduction: Tumours of the maxillofacial region with an orginthat is closely related to tooth formation are called odontogenic tumours. Both odontogenic and non odontogenic tumours can have malignant variants. In Tanzania, oral malignant tumours constitute 50.53% of neoplastic oral facial cases. At the inception most of the tumours occurring in the maxillofacial region are painless. Aim of the study: To determine the factors associated with late reporting for health care among patients having oral maxillofacial tumours or tumour-like lesions attending Muhimbili National Hospital. Material and Methods: A descriptive cross sectional study was conducted, which involved 151 patients with either tumour like lesions or tumours of the oral and maxillofacial region. The study was conducted at the oral maxillofacial firm of Muhimbili National Hospital and oral maxillofacial department of the Muhimbili University of Health NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 81 and Allied Sciences. Information was gathered using structured questionnaire translated in Swahili language. Data was analyzed using SPSS version 20. Ethical clearance was obtained from the Ethical committee of Muhimbili University. Results: Study finding revealed that 55.6% of the patients lived near to health facilities whoich had no oral health services. Ameloblastma was the tumourthat was seen with the highest (21.9%) frequency followed by squamous cell carcinoma (14.6%) and ossifying fibroma (6.6%). Patients with ameloblastpoma showed more delay in reporting to a health facility as compared to patients with squamous cell carcinoma. After referral from the primary health facility, (23) patients reported to the referral centre after one month from the date of reffera;l. Among these 8 (34.9%) reported after one year or more after referral. Lack of perception of the lesion as being a problem, costs involved for transportation and treatment, lack of pain, attending to traditional healers and the use of herbal medicines were found to be the reasons for the delay in reporting for health care among patients with oral maxillofacial tumours. Vast majority (98%) of the patients seen have never had any dental check-ups. Conclusion: The patients with oral and maxillofacial tumours showed a delayed reporting to primary health facilities and consequently to the referral centre. The average time for reporting to the referral centre was rather long (69 weeks). The main reasons cited for the delay in reporting for healthcare by this group of patients is the low level of awareness (did not perceive the lesion being a problem). Cost involved for transportation, treatment and purchase of drugs and absence of pain from the lesion. Recommendations: There is need for education to the communities on the importance of early reporting whenever they suspect a lesion in the oral and maxillofacial region. Continuing education should be provided to the practicing oral and general health personnel who are working in the regions. There is a need for a more extensive study involving more regions and referral centres in the country. 82 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Abdala, R. M. MSc. (2013) The experiences of informal care givers in caring for patients with cancer at Ocean Road Cancer Institute, Dar es Salaam, Tanzania Abstract Background: Cancer is a major chronic problem which affects many people directly or indirectly. It causes much suffering among patients and families. Cancer patients require long-term treatment and a continuing need for care. Consequently, informal caregivers in various settings are now taking on the responsibility of caring for patients with cancer. Such informal caring may involve physical, psychological and economic stresses. In Tanzania, informal caregivers have become so essential that they provide large part of the care needed in hospitals and at home. Aim: The aim of this dissertation is to understand the experiences of informal care givers in caring for cancer patients at Ocean Road Cancer Institute in Dar es Salaam, Tanzania. Method: Study design was explorative qualitative study. The sampling technique was purposeful sampling. Data was collected through face to face interview and focus group discussions. A sample of seven (7) and ten (10) informal care givers participated in face to face interview and two focus group discussions respectively. Content analysis approach was used to analyze the perspectives of the informal caregivers. The study took place between May and June 2013. Findings: Caregivers of patients with cancer experienced many problems including psychological such s stress, and worries. They were disappointed with long waiting time for radiotherapy services at the hospital. They also experienced social problems such as inadequate social support during hospitalization such as food and lodging for caregiver from up country. The time spent in the hospital resulted into less time devoted for child care back home. Most participants stated that the task of taking care of the patients interfered with their daily activities which increased the economic problem they already had. In order to cope with the caring responsibilities, the participants desired to have professional, social, and financial support from the Government and Non-Government Organizations. Conclusions: Caregivers of patients with cancer experience many programme such as psychological, emotion, economical and social problems. These problems are influenced interpersonally, interpersonally and communally. Professional assistance, public awareness social and financial support by the government and NGO’s are important to enhance coping strategies. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 83 Recommendations: The government should put more effort on buying another radiotherapy device to reduce the length of hospitalization waiting for therapy and hospital management should support caregivers by proving food and lodging those caregivers who are coming from far regions and they lack relatives to support them in the city. Keywords: Care giving, Informal caregivers, Experiences, Cancer Mageta, D. J. MSc. (2013) Assessment of knowledge, attitude and practice of healthy timing and spacing of pregnancy among women attending antenatal clinic at Muhimbili National Hospital, Dar es Salaam Abstract Background: Healthy timing and spacing of pregnancy (HTSP) is a concept which was introduced by WHO in 2006 after a review of literature concerning pregnancy spacing and outcomes. HTSP sets the optimal timing of conception and spacing of child birth after a live birth, miscarriages or induced abortion. But is unclear to what extent women in reproductive health clinics in Dar es Salaam perceive and practice this evidence based concept. Objective: Assessment of knowledge, altitude and practice of healthy timing and spacing of pregnancy among women attending ANC at MNH. Study Design: Descriptive cross section study. Study area: The study was conducted at Muhimbili national Hospital in Dar es Salaam. Study duration: Three months from August 1st to 31st October 2012. Study population: Pregnant women attending ANC clinics at MNH during study period. Data collection: Structured questionnaire were used to collect data on knowledge, attitude and practice on HTSP. Data analysis: All responses were coded, entered into SPSS version 16 computer program for data analysis in accordance to specific objectives. Data was analyzed and interpreted in terms of frequency/ proportions determination and cross-tabulations. Comparison of continuous data was done using student’s t-test and Chi test was used for proportions. In all statistics a P value of <0.05 was taken as significant statistically. Results: Majority of women were of age group of 19 to 29 (n=210 49.2%) and the mean age was 29 years. Proportion of women who got their first pregnancy at age above 18 years was 90.4%, and who spaced their index pregnancy according to HTSP 84 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS recommendations were 53.4%. Women who had history of using FP methods in their life time had high knowledge (83.3%) and positive attitude (62.3%) on HTSP. Conclusion and Recommendations: Practice on timing was good; women had high knowledge on timing and spacing of pregnancy after live birth. There were positive attitude on healthy timing and spacing 2 to 5 years while more than half had negative attitude on the risks which may occur when women space more than 5 years. History of using modern FP methods was poor; history of FP use had an impact on knowledge and attitude on HTSP. Therefore messages on HTSP should be disseminated at health care clinics, mass Medias and on outreach programmes. Chipeta, P. MSc. (2013) Prevalence of cardiovascular autonomic neuropathy in diabetic patients attending Muhimbili National Hospital DIABETIC CLINIC Abstract Introduction: Cardiovascular Autonomic Neuropathy (CAN) is a common chronic complication of Type 1 and Type 2 diabetes mellitus. It is however, one of the most overlooked of all serious complications of diabetes that is characterized by deterioration of autonomic fibers innervating the heart; blood vessels and different organs. The consequence of this enervation is postural hypotension, left ventricular dysfunction, painless myocardial infarction, ischemia and sudden death. For this reason CAN results in significant morbidity and mortality in diabetic patients with its prevalence varies between 1% to 90%. The early detection of CAN is essentially important in-order to appropriately manage symptoms and prevent disease progression. Its diagnosis can be quickly and noninvasively made by measuring the standard autonomic function tests as described by Ewing’s criteria. Objective: This study was conducted to determine the prevalence of CAN in diabetic patients attending the Muhimbili National Hospital (MNH) diabetic out-patient clinic. Methodology: It was a descriptive cross sectional hospital based study among diabetic patients attending the MNH diabetic out-patient clinic. CAN was assessed by using five autonomic function tests by Ewing’s criteria. These were, resting heart rate, beat-to-beat heart rate variation, heart rate response to standing, systolic blood pressure response to standing and diastolic blood pressure in response to isometric exercise. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 85 These autonomic function tests were then scored and assigned to three categories; early CAN if there was only one autonomic dysfunction, definite CAN if there were two abnormal tests and severe CAN if three or more tests were abnormal. CAN was described to be present when there was at least one abnormal result detected. Information was recorded using a structured questionnaire and data analyzed using SPSS version 20. Results: The prevalence of CAN was found to be 100% (N=270) within the study group (based on at least one abnormal function test) using Ewing’s criteria in a population comprising of 20.4%Type 1dibetic patients and 79.6% Type 2 diabetic patients. The most predominant type of CAN was Definite CAN seen in 44 (80.0%) Type 1 diabetic patients and 164 (76.3%) in Type 2 diabetic patients. The severe type of CAN was detected in 2 (3.6%) of Type 1 diabetic patients and 11 (5.1%) of Type 2 diabetic patients. The patients include in the study had an age between 18-85 years with the mean (SD) of 53 (12.73) years. There was a positive association between increasing age and CAN (p = 0.045) with the majority of patients affected 156 (57.8%) being in the 36-60 years age group. Poor glycaemic control assessed by high fasting blood glucose showed a positive association (p = 0.013, N = 105) with CAN. There was no significant association of CAN with other risk factors such as duration of diabetes, hypertension, smoking and a high Body Mass Index. Conclusion and Recommendation: All diabetic patients studied at the Muhimbili National Hospital were found to have at least one form of CAN. This can be attributed to the study population being much holder (mean age 52.5 ≠12.7 years) and the high proportion of poor glycaemic control which was evident from the high fasting blood glucose levels. Because of this high prevalence and the known high morbidity and mortality associated with CAN, it is recommended that screening for autonomic dysfunction be tested in all Type 2 patients at regular intervals say 6 months in-order to detect, risk stratify and treat the dysfunction early, therefore preventing disease progression. Kalinga, L. M. MSc. (2012) Distribution of anti malaria medicines in public health facilities in Iringa Region Abstract Background: Malaria is a major threat to public health in Africa that puts approximately 3.3 billion people at risk in 109 countries around the world. Tanzania has the third largest population at risk of malaria, with the disease causing 60 – 80,000 deaths per year. 86 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Inadequate supply of anti malarias at the health facilities especially in rural areas is a major barrier to effective management of the disease. This can lead to high morbidity and Mortality rate in the country. Main objective: To assess the distribution of ant malarial medicine in the public health facilities in Iringa region. Methodology: A cross sectional descriptive survey was conducted involving 56 health workers from the selected 4 hospitals, 11 health centers and 12 dispensaries in Iringa region. Data were collected using questionnaires and by observation. SPSS version 16 was used for data entry, cleaning and subsequent analysis. Results: Thirty two (32%) of all health facilities visited did not have all types of anti malarial medicines on the day of visit. The days of anti malarial medicines stock out at each facility for the last six months (October 2011 to March 2012) was 62 out of 180 days. The main causes of stock-out were unavailability of Alu at MSD from October 2011 to March 2012, non conformity of quantities delivered to orders from MSD, delays in delivery and errors in forecasting at the health facilities. Most of health facilities had poor record keeping with the overall acceptable inventory management of 67.82%. Generally, the average percentage score of storage conditions of all health facilities visited was 57.2%. There is no association of quality storage condition with the level of the health facility. Expiries of different anti malarial medicines were very few, found only in one hospital and one health center. Only 6.8% pharmaceutical inventory managers interviewed were pharmaceutical personnel by profession, and the rest are from other health professionals. Conclusion: Basing on the elements of effective distribution, it can be concluded that: There was poor distribution process for anti malarial medicines at the health facilities visited which lead to un availability of these medicines. These was due to:- Frequency unavailability of the anti malarial medicines at MSD, lack of pharmaceutical inventory management knowledge of the health providers and inadequate supervision of health facilities by council Health management Team (CHMT) lead to poor recording and unacceptable storage condition. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 87 ARDHI UNIVERSITY (ARU) Siwa E. N. ( 2013); Assessing impacts of climate changes for improved beef cattle production using remote sensing and GIS technologies: A case of Ilemela and Magu Districts in Mwanza Region – Lake Victoria Basin, Tanzania Abstract This study focused 0n contributing to the unknown spatial explicit knowledge on the impacts of climate change to beef cattle production, by assessing impacts of climate change on rangelands of the Lake Victoria Basin (LVB) using Remote Sensing and Geographic Information System so as to establish a strategy for adaptation to climate change for improved beef cattle production in Lake Victoria Basin area. The study used supervised classification of satellite imageries in ERDAS Imagine software to analyze land cover changes in the year 1980, 2000 and 2010. It was revealed that settlements and cultivated areas increased, while riverine vegetations and woodlands declined. Participatory mapping and Focus Group Discussions were used to assess spatial changes in stock routes in relation to water availability and pasture. It was further revealed that there were many changes in stock routes such that some have became roads, some were lost and other narrowed. These changes were due to urbanization, and more specifically a general decline of forage land. Furthermore, the study assessed trend of Beef Cattle Production against rainfall and pasture. It was observed that in Ilemela District there were an increase in pasture, and decline in rainfall and beef production over the study period. While in Magu District, there were an increase in beef cattle production and decline in rainfall and pasture over the same period. This implies that climatic changes impacts are more severe in Ilemela than in Magu District. It is recommended that there should be intensification of beef cattle production, Land Use Planning, education on the effective cattle farming and intervention by Rainwater Harvesting as a strategy for adaptation of climate change effects for improved beef cattle production in Lake Victoria Basin Area. 88 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Hatibu, H. M. MSc. (2012) Investigation on production of sandcrete blocks in Dar es Salaam Abstract This research study investigates the production of sandcrete blocks in Dar es Salaam. The study appraised its objectives by using sampling, field study work and laboratory experimentation. Twenty one (21) commercial sandcrete blocks factories were randomly selected, six (6) from Ilala, seven (7) from Kinondoni and eight (8) from Temeke Municipality. Five samples of sandcrete blocks were randomly taken from each selected factory. Site observations and interviews conducted to owners, artisans and supervisors, were the methods used to collect data for his study. Other methods used for data collection results from laboratory experimental tests (compressive strength and water absorption tests) which were conducted on randomly selected blocks. Other laboratory test results used, were sieve analysis test, which was conducted on the soil samples used for sandctrete blocks production. The result from this study revealed that when the fine aggregate used for block production, the water absorption attained by blocks were between 5 and 16.6 with more than 85% blocks falls below 15% which is maximum limit recommended by the standard. The compressive strength of the sandcrete blocks were between 0.17 N/mm2 and 2.4 N/mm2 with all blocks falls below standard recommended by British Standard (BS) and Tanzania Bureau of Standards. Proper curing, suitable mixing ratio and simple tests of materials on site were suggested to improve the quality of sandcrete blocks. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 89 Twimanye, J. M. MSc. (2013) Assessment of the contribution of housing cooperatives in the provision of affordable housing: The case of Muungano Housing Co operative Society- Chamazi. Abstract House delivery through Housing Co-operatives has been practices in several countries of the world in solving housing problems. It has so far become a dependable house delivery strategy due to the fact that house construction is highly capital intensive and not many people can afford can afford to build from their own individual financial sources. Housing co-operatives in Tanzania came into operation in the late 1960s and early 1970s and have been very instrumental in various parts of the country where people from low and middle income brackets have been housed. Mwenge, Sigara, and Mapinduzi Housing Co-operative Societies are among the urban neighborhoods popularly referred to as urban villages. House delivery through housing co-operatives exhibits affordability through bulky procurement of construction materials and technical expertise, involvement of members in the do-it-yourself, and improved efficiency through repetitive works in house construction. Despite various obstacles that the co-operatives are facing, this study has found, through the Muungano Housing Co-operative Society, underlying potentials in delivery of affordable houses. The co-operative society, in its three years of existence, has already housed fourteen households in its target of housing 250 households. Inner motivation of members and effective member participation has been among its success factors. From this study, it has been evident that the objective of achieving affordable housing is possible. However, the Muungano Housing Co-operative Society’s experience indicates that for housing co-operatives to effectively succeed in their endeavors there is a need for the government to intervene and assist in easing the threshold on housing finance and land availability. 90 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS UNIVERSITY OF DAR ES SALAAM Saria, Anasia MSc. (2013) Impact of biofuels investment on livelihoods of the rural community in Kisarawe District. Abstract This study assessed the impacts of biofuels investment on livelihoods of the rural community in Kisarawe District where two villages Muhaga and Mtamba were studied. Data were collected through the use of combination of the methods: households’ survey questionnaires, key informant interviews, focus group discussions, direct field observation, and reviews of relevant literatures. Landsat MSS satellite images of 2000 and 2010 were used to assess land cover changes around the biofuel investment area. Data were also analysed through theme –content technique and with the help of the Statistical Package for Social Sciences (SPSS) and findings presented through percentages, tables, graphs and charts. The findings show that about 8,211 ha (2.3 percent of the total district land area) was initially granted to Sun Biofuel (SBF) investor for biofuel plantation which was later acquired by another company, Thirty Degree East (TDE). The study revealed that during the land acquisition, the investor made several promises to the villagers. However, most of the promises were not fulfilled. It was also found that, in compensation for affected individuals, the land valuation process was not clear and it lacked transparency. Satellite images analysis revealed that the biofuel investment has caused environmental destruction in Ruvu Forest Reserve as well the new land has been opened up for cultivation around the plantation area. This study concluded that, apart from employment, biofuel investment in Kisarawe District has yet to yield the expected impacts in improving the livelihoods of local communities. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 91 As such, the study recommends that the government should ensure that land acquisition for biofuel development would be acquired through open and transparent ways after thorough sensitization and consent of host communities. Rwegoshora, S. A. MSc. (2012) Applicability of performance measurement during product development in manufacturing industries in Tanzania Abstract This dissertation presents a study on the applicability of performance measurement during product development in manufacturing industries in Tanzania. The use of performance measurement has been increasing and has made revolution in manufacturing industries worldwide. However, in developing countries like Tanzania, application of performance measurement is still primitive, compared to what is done elsewhere. This result in production of inferior products that are usually out - competed by products from other countries. This study utilized both qualitative and quantitative research approaches. Data were collected through questionnaire and interview with total of 30 respondents, and then was analyzed by using statistical package for social sciences (SPSS). The results show that the level of application of performance measurement of the employees in manufacturing industries in Tanzania ranges from low to moderate. This implies that no emphasis is placed on performance measurement. The type of performance measurement that is done is based on cost and time only. The main barriers in the implementation and application of performance measurement are lack of resources, lack of support for their use, lack of company accountability, little knowledge by the management on how to performance measurement and lack of a defined system that in place for performance measurement in manufacturing industries. Moreover, performance measurement guidelines was developed that can be used in manufacturing industries for better performance and in the improvement of the quality of products that are manufactured. 92 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Tunga, M. MSc. (2014) Improvement of information flow in service delivery to teaching personnel: A case of Teachers Service Department in Tanzania Abstract This study was conducted to address challenges in information flow, storage and retrival within teachers’ management offices which are Teachers Service department (TSD) and related stakeholders especially the Local Government Authority (LGA). These are sectors where a lot of complaints have been fired against the bureaucratic delays in services delivered to the teaching personnel, inconsistency of data found at different levels, anomalies in updating the information and repeated turnover. The study also involved designing and developing of an extended system that provides central access to teaching personnel information in order to address information flow challenges. A qualitative research technique was employed through triangulation of interviews, participants’ observation, document analysis and software prototyping in order to capture challenges in the information flow in providing services to teaching personnel. Object oriented analysis and design approaches were also used to add TSD component in the current Local Government Human Resource Information System (LGHIS). During this study extended LGHIS was deployed at Ilala District, Dar es Salaam. The main contribution of this research is the streamlining of teachers’ information flow implemented through extension of the LGHIS system by addition of TSD component. The extended system provides central access to teachers’ information thus eliminates anomalies of data found at different echelons of TSD and LDA by enhancing data integrity, consistence and provides complete view of data. The extended system also generates TANGE (Seniority List) reports, streamlines storage, updates, records movements between TSD and LGA and synchronization of the teachers’ information. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 93 Mbwilo, B. M. MSc. (2013) Development of online sustainable energy experiments using emona helex on ilab shared architercture with switching capability Abstract Laboratory is important in Engineering and science as students get chances to practice what they have learned in classes. However there is no laboratory component for students undertaking courses in sustainable energy at University of Dar es Salaam. Introduction of these labs is expensive as new apparatus should be bought; building for lab should as well be available and staff to manage the labs. Another problem is number of experimental setups that can be available online at a time. Sustainable energy experiments were developed on EMONA HELEx kit, switching mechanism was incorporated on the kit since it is designed to do one experiment at a time. The DIO lines on the Universal base board of the EMONA HELEx kit together with the digital writer on the ELVIS II +, allowed more than one experiment to be wired at a time. The user interface was designed in such a way that experimenter could select an experiment to perform with the input and output tabs as well. Results for solar cell experiments showed that maximum power of the cell was at the knee of the I-V curve. Electrolyzer experiment showed that current increased exponentially with increase in voltage. Hydrogen fuel cell experiment, three regions on the V-I curve were observed, the activation region, the Ohmic region and mass transport region. To conclude the online sustainable energy labs are necessary especially with the current situation where energy is limited. Recommendation for this research is, other sets of experiments should be integrated on iLabs in order to be available online. 94 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Ngajilo, F. MSc. (2013). Rural-Urban migrants’ contribution to the livelihoods of rural communities in Makete District, Tanzania Abstract The study discusses the influence of rural-urban migrants to the socio-economic development of rural communities in Makete district, Tanzania. The objectives of the study were as follows: to determine the extent of rural urban migration in Makete district, to examine the contribution of individual rural –urban migrants to the wellbeing of the people in their rural homes, to assess the contribution of rural-urban migrant’ home associations to the development of their rural communities; and to identify the limitations that rural-urban migrants encounter in making contributions to their home areas. Data were collected by the use of interview, direct observation and documentary review. Both rural and urban respondents provided information. Questionnaires, interviews and observation check lists guided data collection process. The collected information were analysed by the use of Microsoft excel and Microsoft Statistical Package for the Social Sciences (SPSS). Data analysed were presented in statements, frequency distribution tables, charts and pictures. Finding from the study revealed that males dominate the migration process. The study observed that individual migrants send consumer goods like clothes and food stuffs as well as money to their rural households. Migrants’ groups contribute in improving buildings and other infrastructures like schools, churches, offices, health centres, bridges and roads. On the other hand the study indicated that poor infrastructure, individualism and lack of trust constrain migrants’ process of making contribution to their rural home areas. However, the study recommended that the central government should improve rural infrastructures such as roads. Also, local government leaders should be devoted to the development of rural communities. Again, rural people, individual migrants and groups of migrants should be patriotic to the development of their rural homes. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 95 Jabir, J. A. MSc. (2013) Cooperation between ministrial information offices and the mass media in disseminating information on public goods Abstract This study examines the cooperation between ministerial information offices and the mass media in dissemination of public goods information. The study was conducted in Dar es Salaam region where most of the mass media and Ministerial information offices exist. It employed qualitative (key information interviews and observations) as well qualitative (questionnaires) research design to analyze the cooperation between the selected ministerial information offices and the mass media in the dissemination of information on public goods. Purposive and random sampling techniques were used to select a sample of the respondents. Key findings revealed that there is no formal cooperation between ministries and the mass media in the dissemination of information on public goods. The Ministries and the mass media do not have research activities on public goods issues and that is why there is little coverage on such endeavors. The bureaucracy and culture of secrecy in the Tanzania government system contribute to making it difficult for ministerial information officers to be accepted as team members in their system. Some senior officers see them as a threat due to their relationship with the mass media. This has to change so as to bring the mass media and government together in the provision of information and knowledge to the public. Sources of information including experts’ involvement have to be increased and emphasized. The study recommends that since information and knowledge sharing on public goods is crucial for national development and socioeconomic status of the public, more training and awareness programs for the ministries senior officers is needed for them to understand and value the existence and needs of information officers; expert use and consultation of specific sources of information is overemphasized. It is also high time for both the government and the mass media to formulate a policy and monitoring body to oversee the dissemination of information on public goods as required unlike what is happening currently whereby very little is covered and no one is directly responsible. 96 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Kira, E. S. PhD. (2014) Integrating of meteorology and environmental education in the certificate of secondary education geography syllabus in Tanzania Abstract The purpose of this study was to explore the certificate of secondary education geography syllabus in order to address the opportunities and challenges regarding the integrated concepts of meteorology and environmental education. The study employed mixed methods approach where by both qualitative and quantitative data were collected. The respondents were 480 students and 24 teachers from 12 schools in Morogoro region including also an expert of geography curriculum development. The findings revealed that: The current geography syllabus for secondary schools introduces the basic concepts of meteorology and environmental education including the causes and effects of climate change. But issues of climate change are treated in isolation without linking them with the concepts of human activities. Problems observed during implementation of the geography syllabus were: teachers’ insufficient content and pedagogical knowledge, lack of textual and non-textual resources that address the relevant concepts comprehensively, and presence of some misconceptions in the most utilized school textbooks. Students could relate some aspects of environmental degradation with climate change by they had some misconceptions on the causes of climate change and in relating the same concepts with conservation techniques and power consumption. It was further observed that students from the urban schools were more knowledgeable on the surveyed concepts of meteorology and environmental education than rural students due to limited sources of information in rural areas. Also, boys were more knowledgeable than girls on the same concepts. This could be associated with girls’ negative attitudes towards science subjects. Based on these findings the Ministry of Education and Vocational Training should ensure that, concepts of meteorology and environmental education in both the geography syllabus and school textbooks focus on raising the students’ awareness on the issues of environmental management, vulnerability of the country to the effects of climate change, necessity of utilizing weather and climate information and the relationship between forest destruction and depletion of water resources under the influence of climate change. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 97 Also, the ministry should ensure that both in-service and pre-service teachers should be equipped with the necessary knowledge and skills so that they can effectively teach the concepts of meteorology and environmental education. However, Tanzania institute of Education may combine other concepts of environmental management with relevant social-scientific concepts so that they can be more appropriately dealt with in a separate subject. Mahongo, S. B. PhD (2014) Analysis of met-ocean processes in relation to changes in large scale climatic systems in Tanzania and the Western Indian Ocean Abstract The recent changes in world climate have highlighted the need for a proper understanding of the factors that influence changes in met-ocean processes in Tanzania and the western Indian Ocean. The region is unique in that these changes have generally not been fluctuating in consistence with the global change processes, thus posing a notable scientific challenge. This thesis addresses how these processes have been changing overtime in the region, and how they are associated with the large scale climatic phenomena. The thesis also discerns the oscillatory patterns of the met-ocean parameters, and investigates their potential for prediction. The study is implemented through wavelet and spectral analyses, multivariate linear regressions and other statistical tools, while prediction is carried out through artificial intelligence and linear time series modeling. Key findings of this study include the revelation of significant warming trends of surface air temperature, general strengthening of winds, decline in rainfall, and the presence of a quasi-22 year cycle on the low frequency timescales of winds and sea level, which mimic the Hale solar cycle. The influence of the 22-year rhythm is larger in amplitude than those due to large scale climatic systems. At the Island of Zanzibar for instance, the short sea level record of 1984-2004 largely resonated with the 22-year rhythm thus leading to a spurious negative trend. However, the relationship between solar activity and met-ocean processes is complex and varies with time and space such that this pattern cannot be generalized for other locations in the region. This thesis has also revealed that neural networks and state-of-the-art time series models are capable of making excellent predictions of sea surface temperature, thus providing potential for prediction of other met-ocean parameters. Determination of low frequency oscillations in met-ocean parameters is dependent on the length of the record, with about 1090 years of data being nweden for any conclusive evidence on trends in 98 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS climate. Although ocean monitoring is currently possible through satellites, instrumental monitoring is still needed for calibration of the satellite sensors. Continued in- situ and satellite monitoring of the met-ocean parameters is therefore essential for a better understanding of the processes, permitting sound prediction and enabling proper planning, adaptation and resilience. Shoko, A. P. PhD. (2013) Aquaculture of Nile Tilapia (oreochromis niloticus linnaeus, 1758) with an assessment of its economic benefits in the Lake Victoria basin, Tanzania Abstract Fish stocks in Lake Victoria have drastically declined while the demand for fish has exponentially increased, leading into inadequacy of fish. Aquaculture has the potential of bridging the widening gap created by the increasing demand. Despite the enormous potential for aquaculture development in the Basin, this sub-sector is still underdeveloped. This thesis examines the aquaculture of Nile tilapia (Oreochromis niloticus) and assessed its economic benefits in the Basin. Assessment on fish pond performance suggests that, despite low fish yield due to poor pond management, aquaculture offers an opportunity for increased productivity. Investigations on diurnal dynamics of water quality parameters showed that dissolved oxygen and un- ionized ammonia were beyond the acceptable limits for fish growth suggesting a need for frequent water quality monitoring in ponds. The effects of integrating fish farming with vegetables on fish growth, yields and economic benefits revealed higher performance from integrating than from non-integrated system, pointing to the importance of integrating fish farming with other on-farm activities. The effects of stocking density on production and economic benefits in polyculture and monoculture systems were assessed. It is suggested that the choice of an optimum stocking density is an important aspect in attaining higher performance in any culture system. Investigation on the reproductive biology of Nile tilapia showed that its early breeding behavior in captivity is not affected by culture system and thus, its production can be improved through proper pond management. The practical application of the results is discussed in detail in each chapter and a synthesis is presented at the end of the thesis. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 99 THE NELSON MANDELA AFRICAN INSTITUTION OF SCIENCE AND TECHNOLOGY (NMAIST) Ramadhani, A. MSc. (2013) Mobile SMS based framework toward enhancing veterinary extension service in rural areas of Tanzania Abstract Veterinary Extension service is the part of Agricultural Extension service which deals with provision of education related to livestock keeping from veterinary officers to livestock keepers. The education provided includes advices, skills and techniques on how to increase livestock resources and how to control and prevent livestock diseases. The existing system for providing veterinary extension service in Tanzania is linear i.e. information flows from Ministry of livestock and Fisheries Development to veterinary officers and finally to livestock keepers. Linearity of the existing system, inaccessibility of rural areas and shortage of veterinary officers contributes to inefficient provision of extension services in rural areas of Tanzania. The research investigated how Mobile SMS based Framework can be used to increase accessibility to veterinary extension services in rural area of Tanzania. Review on the existing system and views on the proposed system were accomplished through questionnaires, interviews and by reviewing different published and unpublished materials. Mobile SMS based framework was designed based on livestock keepers’ requirements. Prototype was developed by using Mysql, Ozeki SMS gateway, GSM Modem and SQL yog to test the proposed framework. The designed system is of great help to livestock keepers and Ministry since; it save time in providing veterinary services, it minimize cost since few livestock officers can be employed and livestock officers are able to get extension service on time. Also publication of this research enables other researcher to work on the same technology. 100 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Nyamtiga, B. W. MSc. (2013) Enhanced security controls for mobile banking systems in Tanzania Abstract Mobile banking schemes dealing with provision of banking and financial services using mobile devices face a number of security threats. Most technologies used in conducting mobile transactions utilize GSM services, and financial institutions tend to rely on the security provided by the communication networks for data protection. The transactions become vulnerable to attacks because of the security flaws that exist in these networks. Among the most significant vulnerabilities include the authentication and encryption algorithms used. These algorithms have been breached and if data is transmitted without adequate protection it becomes susceptible to cryptanalysis attacks. Thus, service providers should not rely only on communication systems for data security. Some additional measures are needed to cater for enhanced security when transmitting sensitive financial details. This study explored the current technological and security issues in mobile banking systems and ways with which these systems can be made more secure. Ultimately, an SMS based model is designed with improved security features incorporated. Using JAVA programming and MySQL database; a mobile client and its corresponding server application were developed with enhanced security controls. Messages exchanged between the two components were then sniffed using wireshark network analyzer. The implemented prototype demonstrated an increased level of data confidentiality, improved message integrity and user authentication. Consequently; user details are securely captured on the phone, messages exchanged between client and server are encrypted and their integrity checked, and PIN usage is controlled for effective authentication. This ensures safety of customers’ transactions and improves customer satisfaction. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 101 Julius, B. MSc. (2013) Mobile phone based information system for maternal health care in Tanzania Abstract Accurate and timely information is one of the important components of the public health in order to improve the quality of their services. The rapidly emergent use of mobile technologies has increased pressure on the demand for mobile phone based information system solutions to bridge information gaps in the health sector of the developing countries such as Tanzania. This study analyzed the existing manual or paper based system for Maternal Health Care, and found that the kind of information and education given follow the national guidelines. In this context the greatest challenges were to communicate simply and clearly this information to clients and to manage the clients information. Therefore this master thesis presents the mobile phone based information system for maternal healthcare that will be used to remind clients on their important visits and provide them with basic information that will help them to take care of themselves; also the system will help the community health workers at hospitals to manage the patient information. It will make use of the Short Message Service (SMS) to provide reminders and educational information. Waterfall Model was used to implement the system while data were collected through in-depth interview, observations and document review. Additionally the system was modeled using the UML. Mobile phone based information system for maternal healthcare can position itself as a major breakthrough approach to educate women on pregnancy, monitor their own and child’s progress, follow up with medical checkups and critical updates through mobile phone. 102 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Matumo, M. B. MSc. (2013) Wireless sensor network for electric distribution system power quality monitoring Abstract Power-quality events are of increasing concern for the economy because today’s equipment, particularly computers and automated manufacturing devices, are susceptible to these imperceptible voltage changes. A small variation in voltage can cause an equipment to shut down for long periods, resulting in significant business losses. This study describes the design of the complete electrical sensing system, comprising of an Input-Output (IOIO) Microcontroller Unit which connects to an Android Smartphone and the GSM network that can be adopted for power quality monitoring at the electric distribution networks (Tanzania Electric Supply Company, TANESCO, a study case) in order that the primary power faults parameters can be communicated to respective control centers and electronically stored for further analysis. This system is designed to improve the accessibility of power-quality information and to increase understanding of the growing importance of electricity reliability and power quality to the economy. Issues that should be taken into consideration in developing a larger, potentially nationwide, network of power-quality sensors were identified. The study is centered on how to detect an event and or a disturbance and communicate an alert of an event that cause fault in the electric distribution line to a control center and how to electronically store such information for further analysis. Currently, TANESCO’s distribution network has no fault parameters being communicated (relayed) to control center. Control centers rely on the information from customers. Unless customer informs customer’s service desk, the fault go unreported. Communication of faults at distribution networks is imperative because its absence leads to disruption and damage to electrical appliances and equipment coupled to the power system, and hence, to economic losses and even danger to life and health of service staff. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 103 Ngatunga, D. P. MSc. (2013) Diversity of plasmodium malaria and molecular markers of sulphadoxine resistance in Muheza, Nachingwea and Muleba Districts Abstract This was a cross sectional study (CSS) on the diversity of Plasmodium malaria parasites and molecular markers of Sulphadoxine resistance in selected sentinel sights with different transmission settings. It included Mkuzi site in Muheza district (routinely used site by National Malaria Control Programme to monitor the efficacy of antimalarials) and hyper- endemic and holoendemic sites in Nachingwea and Muleba districts respectively (based on Tanzania HIV and Malaria information system data). The aim was to show distribution of the burden and mapping of the risks of malaria in different epidemiological patterns. Patients were screened using MRDTs to initially detect infections and positive cases confirmed by microscopic method. Molecular markers of Sulphadoxine resistance at K540E and A581G codons of parasites’ gene were detected by genotyping method preceded by SSOP-ELISA. P. falciparum prevalence was the most predominant at all sites while P.ovale was only detected at Muleba and Nachingwea. Gametocytes of P.falciparum were only detected at other sites except in Muheza while gametocytes of other species were detected in all sites. Sulphadoxine resistance was significantly higher in Muheza compared to the other sites, which is predicted by high SP usage. High mutation level involving codon 581G at Muheza suggest possible future spread to other sites, exacerbate SP resistance and further limit use of IPTp. Muheza and Muleba sites have high malaria prevalence contrary to previous reports in earlier studies. More studies covering diverse sites are needed to provide a reliable picture of malaria burden in the country. 104 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mwamilawa, E. MSc. (2013) Reduction of post harvest losses of tomatoes by developing tomato powder product Abstract Tomato (Lycopersicon esculentum) is an important vegetable crop because of its usefulness in everyday life in dishes preparation and its protective and curative function in human body. It is a highly perishable crop that requires immediate processing after harvest, and because of that, the losses are high (30 – 50 %) especially in developing countries including Tanzania. There have been several interventions attempting to reduce such huge postharvest losses, but to the moment their impacts have not been realized in Tanzania. This study aimed at developing a powder product of acceptable quality, by using solar tunnel and electric cabinet drier; after pre-treatment with Table salt (NaCl) or Slightly Acidic Electrolysed Water (SAEW). The effects of pre-treatments on Lycopene, Vitamin C and microbiological quality were assessed. Firstly, the two drying methods were studied and their performance evaluated. The electric cabinet drier was the best for tomato drying in and retaining vitamin C, lycopene and reducing microbial load. Secondly, 2 % salt was the best in retaining Vitamin C (26.4 %), lycopene (71.95 % increase) as well as in reducing the number of microbes (57.35 %); followed by 1 % salt and SAEW the least. Hence, 2 % salt was concluded as the best pre-treatment for tomatoes. Thirdly, the electric cabinet drier was determined as the best for retention of lycopene (61.32% increases), vitamin C (25.3 %) and reduction of microbial load (39.7 %). Part four of this study was development of tomato powder using 2 % salt and the two driers. Tomato powder pretreated with 2% salt from electric cabinet drier showed highest scores regarding colour, taste, texture, flavor and general acceptability; followed by tomato powder pretreated with 2 % salt from solar tunnel drier; none treated samples from electric cabinet and solar tunnel drier. Therefore, it was concluded that electric cabinet drier is more effective than solar tunnel for tomato drying, hence contribute to significant reduction of postharvest losses. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 105 Tairo, E. V. MSc. (2013) Effects of bradyrhizobium japonicum inoculation and phosphorus supplementation on growth and nutrient uptake by soybean (glycine max (l.) merril) Abstract A glasshouse and field experiments were conducted at Selian Agricultural Research Institute (SARI) and Tanzania Coffee Research Institute (TaCRI)-Lyamungu, respectively. The aim of the study was to investigate the effect of Bradyrhizobium japonicum inoculants and phosphorus supplementation on growth and chlorophyll accumulation, nutrients uptake as well as yield components of soybean together with profitability of applying inoculants and phosphorus in soybean. The treatments consisted of selected strain of B.japonicum(USDA 110) at two levels (with & without), and 4 phosphorus levels 0, 20, 40 and 80kg P ha-1. Both treatments were replicated four times in a split plot design. Results showed that inoculation with B.japonicum gave a significant increase in leaf chlorophyll content, plant height, number of leaves per plant, number of podsplant-1, number of branches plant-1, stem girth, Leaf Area and Leaf Area Index, number of days to 50% flowering and number of days to 50% pod formation, hundred seed weight and final seed yield as compared with the control. Rhizobial inoculation also resulted into significant elevated uptake of some mineral nutrients: N, P, K, Ca, Mg, Na Fe, Cu, Zn and Mn in plant tissues. P supply significantly improved the number of nodule plant-1, stem girth, Leaf area, Leaf Area Index and number of days to 50% pod formation, final seed yield, hundred seed weight, number of podsplant-1 and number of branches plant-1in both the glasshouse and in the field compared to the control treatment. Profitability of soybean was recorded on treatments involving inoculants and phosphorus at moderate levels (20-40 kg P ha-1).P supply significantly increased the uptake of some nutrients such as N, P, K, Ca, Mg, Cu, Fe and Mn in both glasshouse and field experiment. However, the uptake of Zn was significantly reduced by higher P level of 80 kg P ha-1. 106 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Mubito, E. P. MSc. (2013) Assessment of antimicrobial usage and sulfonamide residues in chicken eggs in Dar es Salaam Abstract The objective of this study was to survey chicken farming practices and residues of sulfadiazine and sulfamethazine in commercial chicken eggs from Dar es Salaam. A structured questionnaire was administered to commercial chicken farmers and pharmaceutical outlets to obtain information on antibiotic usage, awareness of withdrawal periods and public health concerns. The survey found that, most frequently used antibiotics belong to the group of tetracycline and sulfonamides. Furazolidone was also found in use though it has been prohibited due to carcinogenic and mutagenic effect. The survey found that, all famers do not observe drugs withdrawal periods, for fears of capital loss. Possibly also because 90% of them were not aware of health effect associated with antibiotic residues. To determine antibiotic residues, 96 eggs were randomly collected from layers production farms and analyzed for sulfadiazine and sulfamethazine residues. Extraction of residues was executed using liquid-liquid extraction method in acetonitrile. Detection of residues was by High performance Liquid Chromatography (HPLC), with UV detector and C18 column. The mobile phase was composed by phosphate buffer and methanol (75:25 v/v). The study found that all eggs contained sulfadiazine at level ranging from 22 to 230 ng/g and 59.4% of samples contained sulfamethazine residues ranging from 0.0 to 94 ng/g. It was concluded that, eggs consumers are at a high risk of exposure to sulfonamide residues due to farmer’s failure to observe antibiotic withdrawal periods and poor enforcement of regulations. Consulted efforts are needed to bring awareness on public health implications associated with drug residues in poultry products. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 107 Elisante, F. MSc. (2013) Allelopathic effect of datura stramonium on the survival of grass and legume species in the conservation areas Abstract The main rationale of this study was to investigate the allelopathic effect of D. stramonium on the survival of grass and legume species; Cenchrus ciliaris and Neonotonia wightii respectively. Different concentrations; 0% (control= distilled water), 25%, 50%, 75%, 100% (aqueous seed and leaf extracts of D. stramonium) were used to determine their effects on seed germination, leaf chlorophyll content, root and shoot length, fresh and dry weight on the tested plant species. The inhibitory effects were much greater in higher concentrations (75% and 100%) compared with control (0%). The results revealed the allelopathic effects of D. stramonium on leaf chlorophyll content, root and shoot elongation, fresh and dry weight of all tested species. The total chlorophyll content of N. wightii was significantly reduced (p<0.001) in all plants treated with both aqueous seed and leaf extracts of D. stramonium. In C. ciliaris, the total chlorophyll content was significantly reduced (p<0.001) for those plants treated with aqueous seed extract and (p<0.05) for those treated with aqueous leaf extract from D. stramonium. However, this study observed that the allelopathic effects were concentration dependent because the inhibitory effects on tested species increased as the concentrations. The results from field survey observed that D. stramonium is highly distributed in Embaruai, Lerai, Lemara rangers post, Empakai, Ndian, Olbalbal, Oldupai and Malanja. The results obtained demonstrated that in Ngorongoro Conservation Area the species was more distributed in Lerai 205,244 m2 (69.57%) and Olbalbal 53161 m2 (18.02). The least infested area was Empakai camp site 1781 m2 (0.60%). Generally, this study had confirmed that D. stramonium affects the growth and survival of grass and legume species that has ecological impacts in an ecosystem. 108 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Namkeleja, H. S. MSc. (2013) Allelopathic effects of invasive plant species; argemone mexicana to growth of selected native plant species Abstract The study investigated allelopathic effect of Argemone mexicana L on germination, growth and chlorophyll contents of Brachiaria dictyoneura L and Clitoria ternatea L. The study also determined the spatial distribution of A. mexicana in Ngorongoro Conservation Area (NCA). The experiments were set in the NM-AIST laboratory and field environments within NM-AIST campus. Seeds of B. dictyoneura and C. ternatea were separately sown in petri dishes and pots, irrigated with different concentrations (0 g.L-1, 25 g.L-1, 50 g.L-1, 75 g.L-1 and 100 g.L-1) of leaf and seed extracts of A. mexicana. The experimental design was a randomized complete block with four replications for each treatment. Germination percentages root and shoot length; fresh and dry weight of each seedling was recorded. Chlorophyll contents of B. dictyoneura and C. ternatea were extracted by using dimethylsulphoxide (DMSO). UV/Visible Spectrophotometer was used to determine absorbance values at 645 and 663 nm that used to compute chlorophyll concentrations. Moreover, mapping for spatial distribution of A. mexicana in NCA was conducted by using Global Positioning System unit. Results showed that seed germination percentage, root and shoot length, chlorophyll content, fresh and dry weight, of B. dictyoneura and C. ternatea seedlings were significantly reduced with increase in leaf and seed extracts of A. mexicana compared with control treatments. Roots were more affected than shoots; and leaf extracts was more suppressive than seed extracts. Furthermore, in NCA, A. mexicana were recorded along the roads, rivers and gravel mining sites. Hence, human activities, water, livestock and wild animals disperse the seeds of A. mexicana. In conclusion, leaf and seed extracts have some allelochemicals with inhibitory effect on germination and growth of the tested plant species. Furthermore, control measures should be strengthened to avoid further spread of this noxious weed within the park and other protected areas. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 109 Laizer, H. MSc. (2013) Demography and ecology of lion population in Tarangire National Park, Tanzania Abstract The study assessed the demography and ecological characteristics of lion population in Tarangire National Park from March to September 2013. Using the long-term data from Tarangire Lion Project and data collected during this study population characteristic including population size, age structure, sex ratio, pride composition and cub survival were determined. Radio telemetry and GPS collar were used to obtain data on home ranges and movement patterns of different prides within the park, these data were compared between wet and dry seasons. Observation was done on daily basis and data were analyzed using STATISTICA. The results showed that the population was estimated to be around 155 individuals as in June 2013 with a density of 7.5 lions per 100 square kilometers. The sex ratio was more in favor of females (1: 1.2). On the other hand cubs had a sex ratio 1:1. The age composition was dominated by pre-reproductive age class (cubs and subadults), which constitutes 63.9% of the whole population. There was a total of 9 prides residing the park, the number of lions in the pride ranges from 2-38 individuals with the mean of 17.2 individuals. Cub survival was high with the average of 70.8% of all cubs born survived to year one. Home ranges were large ranging from 52 Km2 to 616 Km2 with an average of 209 Km2 and extensively overlap. Home ranges and movements were large in wet season and the small in the dry season. Despite these the overall population trend shows a declining curve, which suggest more conservation efforts are needed to make the population stable. Raymond, J. MSc. (2013) Development of high quality sesame energy bar (Simbar) Abstract Sesame is an important neglected crop with potential to increase nutritional security among the individuals in developing countries. It is a remarkable source of essential micronutrients (both iron and zinc) and bioactive compounds (Sesamin and Sesamolin), which are absent in most staple foods. 110 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS However, its consumption as food is very low in East Africa and the quality of the available sesame products is not well known. This study was conducted to develop a high quality sesame energy bar (SimBar) with the aim of increasing options for sesame consumption. For a single bar (SimBar-25 g), a devised composition of 21 g sesame seeds pre-roasted at 200 °C for 5 min, 1.25 g of gelatinized cassava starch, 0.25 g of baobab powder, 1.25 g honey and 1.25 g sugar were used in the development of SimBar. The mixture of all ingredients was baked at 200 °C for 15 minutes. The sensory evaluation of SimBar was conducted for general acceptability of SimBar compared to existing sesame snacks. Additionally, two micronutrients (iron and zinc) contents of SimBar and existing sesame products were analysed. Sensory evaluation results showed that, the developed SimBar was generally accepted and ranked higher than t h e existing sesame snacks. It was also found that, t h e SimBar had the highest content of iron (8.88 mg/100 g) and zinc (5.75 mg/100 g) than the existing sesame snacks, which had an average of 3.76 mg/100 g and 4.03 mg/ 100 g for iron and zinc, respectively. Therefore, the value addition of sesame seeds through development of SimBar can be a promising method for further utilization of sesame crop, improving lives of smallholder sesame farmers as well as improving food and nutritional security. Njisya, J. MSc. (2013) Isolation of antibiotic resistant bacteria from the environment Abstract This study investigated the occurrence of antibiotic resistant bacteria in different environments. The study was conducted in Arusha whereby sampling sites were Murieti (dumping site) and Momera (clean area) whereby 110 soil and water samples were collected. Biochemical test and Disc diffusion were used to identify and test antibiotic susceptibility respectively. Bacillus spp, Staphylococcus spp and Klebsiella spp were the bacteria isolated from the clean sites while Klebsiella spp, Bacillus spp, Corynbacterium spp, Staphylococcus spp, Proteus vulgaris, Shigella dysenteriae, E. coli, Enterobacteria intermedius and Pseudomonas auriginosa were isolated from the dumpsites. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 111 Twelve antibiotics were used to test for resistance, Staphylococcus spp and Bacillus spp isolated from clean site were resistant to Erythromycin, Penicillin-G, Cephalexin, Amoxycillin and Ciprofloxacin, whereas Klebsiella spp, were resistant to Cefotaxime, Ceftazidime, Gentamicin, Cefixime, Amikacin and Ciprofloxacin. All bacterial species isolated from the clean site were susceptible to Ofloxacin. Bacillus spp, Corynbacterium spp and Staphylococcus spp isolated from the dumpsites were resistant to Tetracycline, Erythromycin, Penicillin-G, Cephalexin, and Amoxycillin. Bacillus spp and Staphylococcus spp were resistant to Ciprofloxacin while Corynbacterium spp and Staphylococcus spp were sensitive to Ofloxacin. Gram negative isolates from the dumpsites were resistant to Cefotaxime, Ceftazidime, Cefixime, Amikacin, Ciprofloxacin and Ofloxacin except for Enterobacteria intermedius, Protus vulgaris, Shigella dysentriae and Pseudomonas auriginosa which showed varying levels of sensitivity to Ofloxacin and Gentamicin. Activity of the antibiotics to the bacterial isolates was statistically different (P<0.05). This study has shown that bacteria from dump sites and clean site are resistant to multiple drugs, commonly used by humans. Lukindo, T. E. MSc. (2013) Cellular IMMUNE responses elicited after VACCINATION with the HYBRID-1+IC31® tuberculosis vaccine in HIV infected Tanzanian adults Abstract A vaccination trial for safety and immunogenicity of a new vaccine against Tuberculosis, Hybrid -1/IC31®, was conducted in HIV infected adults in Bagamoyo, Tanzania. A comparison of pre- and post- vaccination responses indicated that higher frequencies of CD4+ T cells releasing IFNγ were detected on respective days as follows: day 14 (p=0.0215), day 70 (p=0.0342) and day 182 (p=0.0149) following vaccination. CD4+ T cells releasing IL-2 peaked on day 70 (p=0.0079) and day 182 (p=0.0038) while CD4+ cells releasing TNFα peaked on day 182 (p=0.0107) post vaccination. In comparison with placebo, the vaccinated subjects showed an increased frequencies of CD4+T cells releasing IFNγ+ with highest values on day 70 (p=0.0333) and IL-2+ on day 182 (p=0.0323). The frequencies of TNFα producing CD4+ T cells in vaccinated subjects were not significantly different from the placebo. However, frequencies of cytokine producing CD8+ T cells were detected to be relatively low in comparison to both controls. 112 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS T cells simultaneously expressing multiple cytokines (polyfunctional) were also generated in post vaccination. CD4+ T cells releasing three cytokines peaked on day 182 (p=0.0448). CD4+T cells expressing two cytokines, IFNγ+ TNFα+ peaked on day 70 (p=0.0413) while IL-2+ TNFα+ peaked on day 70 and day 182 (p=0.0061 and day p=0.0049 respectively). CD4+ T cells producing IFNγ+IL-2+ did not differ significantly from baseline in terms of their frequency. Detection of higher frequencies up to day 182 indicated a significant generation of long lived immunological memory. Although the study subjects were BCG immunised, the immune responses to BCG were not boosted by H1/IC31® vaccination. Generally it was found that, the H1/IC31®vaccine elicits significant cellular immune responses in HIV infected adults. Nyarobi, M. J. MSc. (2013) Prevalence of rift valley fever virus in the serengeti ecosystem, Tanzania: Evidence for inter-epidemic infections Abstract Rift Valley fever (RVF) is a peracute or acute febrile mosquito-borne, zoonotic disease caused by an RNA virus of the family Bunyaviridae, genus Phlebovirus. It usually presents in epizootic over large areas of a country following heavy rains and flooding and is characterized by high rates of abortion and neonatal mortality, primarily in sheep, goats and cattle; and fatal haemorrhagic syndrome in humans. In southern and eastern Africa, t h e epidemics of RVF have been observed at irregular intervals of about 5–12 years. Since the previous outbreak of 2006/2007 in Tanzania, no RVF cases have been reported in the Serengeti ecosystem. An interesting question to epidemiologists is “where is the RVF virus maintained during inter- epidemic periods”? To address the question, this study investigated the prevalence of RVF in wild and domestic ruminants, rodents and arthropod vectors in the Serengeti ecosystem to obtain information on the virus and the disease after the epidemic. A cross-sectional serological survey was conducted to detect the specific IgG antibodies in serum from livestock, wild ruminants and rodents in the Serengeti ecosystem. Furthermore, a quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the RVF virus in pools of mosquitoes collected in the same area. T h e results revealed that 31.4% of buffaloes (n=102), 23.1% of Grant’s gazelle (n=13), 14.5% of Thomson’s gazelle (n=69), 19% of impala (n=21), 20% of hartebeest (n=20), 6.5% of topi (n=31), 2.3% of wildebeest (n=88), 11.6% of cattle (n=593), 4.5% of goats (n=110) and 3.8% of sheep (n=53) were sero-positive for RVF. It was noted NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 113 that a proportion of sero-positive animals including 21.8% of buffaloes and 29% of cattle were born after the end of the last epidemic (year 2007) indicating a certain degree of new infections post epidemic. Of the 224 pools of mosquitoes, six pools (2.7%) including one from Aedes and five from Culex species tested positive for RVFV genome, indicating the presence of RVF virus post-epidemic in the Serengeti ecosystem. The detection of RVFV nucleic acids in male mosquitoes which normally do not obtain blood meals affirms trans-ovarial or vertical transmission. Put together, t h e results from the study suggest that the RVF virus is maintained either independently within the mosquito populations or in both mosquitoes and the susceptible livestock and wildlife. None of the 347 rodents tested positive for RVF antibodies suggests a minimal role of these species in the maintenance of the RVF virus. Daudi, M. MSc. (2013) Physiological stress; the impact of vaccine and malignant catarrhal fever challenge in cattle Abstract There is a lot of information in literature on animals succumbing to disease(s) after exposure to stressors or on the elevated corticosterone hormone levels preceding the succumbing to disease(s). But little information is available on what happens to corticosterone hormones when animals are exposed to a pathogen as a stressor. In this study, the effect of Malignant catarrhal fever viral challenge on the kinetics of corticosterone hormone in vaccinated and un-vaccinated cattle was investigated. Animals were randomly allocated into five (5) treatment groups each containing eight (8) animals. The animals were vaccinated against Malignant catarrhal fever using the attenuated Alcelaphine herpes virus AHV1.0 vaccine in combination with either flagellin or emulsigen adjuvants as follows; Group 1 (vaccine + emulsigen), Group 2 (vaccine + flagellin), Group 3 (vaccine + emulsigen + flagellin), Group 4 (emulsigen only) and Group 5 (flagellin only). All animals were challenged with Malignant catarrhal fever virus (virulent C-500 strain AHV-1 virus) tcid50 on day 77 after the primary vaccination. Stress response was determined by measuring fecal corticosterone levels using ELISA. 114 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Survival from viral challenge was 75% (Group 1), 50% (Group 2), 37.5% (Group 3), 50% (Group 4) and 12.5% (Group 5). The differences between survival curves was significant (p=0.0182). The 2-way ANOVA was used to determine whether the different treatment regime (vaccine, adjuvant, viral challenge combinations) resulted in different corticosterone responses. Results showed that while there was significant time effect (p <0.001), the group and group×time interaction did not have significant effect. Initially in all treatment groups, the mean corticosterone concentrations decreased progressively from baseline to lowest levels on day 56 and then increased sharply to peak levels on day 77 just before viral challenge. However, the mean peak levels were significantly higher than baseline levels only in Group 2 (vaccine + flagellin) and Group5 (flagellin alone). After challenge with live virus, the mean corticosterone levels decreased progressively in all groups from peak levels on day 77 to lowest levels on day133 and then stabilized. The decrease in corticosterone levels after challenge was, however, significant in Group 5 only. Compared to peak levels, corticosterone concentrations in group 5 were significantly lower on Day 133 (p<0.05), Day 147 (p<0.001) and Day 161 (p<0.001). To determine whether there was difference in corticisterone levels between animals that died from the disease and those that survived, the unpaired t-test was used to determine difference. Mean terminal corticosterone hormone metabolites in dead animals (43.36 ± 3.80 ng/g wet feces) were significantly higher (p< 0.05) than in survivors (29.32 ± 4.55 ng/g wet feces) indicating higher stress response in animals with severe clinical signs. Results from this study demonstrate that malignant catarrhal fever virus challenge in cattle decreases corticosterone levels but in general corticosterone levels are higher in animals with severe clinical signs of the disease. Ally, M. S. MSc. (2013) Mycotoxins and heavy metal contamination in complementary foods locally processed in Tanzania Abstract Complementary foods (CFs) are an important source of nutrients in the diet of infants and are among the first solid food stuffs consumed by them; however there is high possibility that these foods can be contaminated by mycotoxins (fumonisins and aflatoxins) and heavy metals (lead and cadmium). The aim of this study was to assess NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 115 levels of mycotoxins and heavy metals in CFs locally processed in Tanzania. The level of fumonisins in CFs ranged from undetectable to 830.57ng/g. This indicates there is high risk of contamination of CFs locally processed in Tanzania. The fumonisins contamination observed in 14 samples (40%) were above acceptable level of 200ng/g, 19 samples (54.3%) showed occurrence of fumonisins below acceptable level and only 2 samples (5.7%) were free from fumonisins contamination. On the other hand, aflatoxins content in CFs ranged from 0.56 to 27.30ng/g. This shows that there is high rate of aflatoxins contamination in CFs locally processed in Tanzania. The concentrations of aflatoxins observed in all samples were above acceptable level of 0.10 ng/g which is the maximum levels of AFB1 for processed cerealbased foods for infants and young children. Out of 35 samples, only three samples of locally processed CFs contained lead and one sample contained cadmium. However, the study found that there is no risk of contamination with lead and cadmium in CFs locally processed in Tanzania. Mrutu, B. A. MSc. (2013) Molecular characterization of maize (ZEA MAYS L) inbred lines and hybrids in southern highlands of Tanzania Abstract Maize is the most important food crop in Tanzania. It provides 60% of the dietary calories and more than 50% of utilizable protein. All the maize varieties in Tanzania have been characterized morphologically prior to their release and registration and there is very limited information regarding their molecular characterization. Therefore, the objective of this study is to characterize the hybrids of maize by using RAPD markers and use the information to assess the genetic diversity and genetic purity. The maize genotypes were collected from Southern highlands of Tanzania along the seed supply chain from the breeder, seed producer and seed distributors. A total of 12 genotypes were used in investigating the genetic diversity while 47 genotypes were used for evaluating the seed genetic purity. DNA was extracted from young leaves and PCR was conducted using fourteen and seventeen primers for evaluating the genetic diversity and genetic purity respectively. A total of 123 bands were produced from 14 primers of which 98 (80%) were polymorphic with 17 primers and 134 bands were produced which were 100% polymorphic. The similarity coefficient 116 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS based on Jaccard ranged from 0.32 to 0.95 and 0.27 to 0.99 for genetic diversity and genetic purity studies respectively. With genetic diversity, the greater diversity was between the inbred lines than the hybrids. With genetic purity, the genotypes which clustered with the parents were regarded as the true hybrids resulting from the cross between those parents. These results indicate that there is high genetic diversity among the maize genotypes in Tanzania and the varieties which were considered to be genetically identical were found to be different. Key words: Genetic diversity, genetic purity, maize, RAPD markers. Ngomuo, M. MSc. (2013) In vitro propagation methods of banana by using shoot tip cultures Abstract The study on in vitro propagation methods of banana by using shoot tip cultures was conducted at Mikocheni Agricultural research laboratory. The study was conducted to investigate; i) The control of lethal browning in local banana variety (cv. Mzuzu) by using ascorbic acid. ii) The effect of different bud splitting techniques on number of shoots and fresh weight produced in vitro culture of (Musa sp.) var. ‘Yangambi’. iii) The effects of auxins and cytokinin on growth and development of (Musa sp.) var. ‘Yangambi’ explants in tissue culture. One experiment consisted MS media supplemented with different concentrations of ascorbic acid, which was also used during explant preparation. Fifteen treatments were used. In another experiment, MS media supplemented with different concentrations of BAP with and without IAA was used in efforts to stimulate in vitro buds proliferation. MS media supplemented with IBA with and without BAP was used for in vitro rooting. Buds splitting technique was also applied fourteen days after intiation, MS media with 5mg/l BAP was used to assess the effects of different buds splitting techniques on in vitro buds proliferation. Ten replicates were used for each experiment. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 117 The results indicated that the use of 1.2g/l of AA during explants preparation was good in controlling the incidence of lethal browning of cv Mzuzu compared with the rest of all other treatments. Also both 100mg/l and 200mg/l of AA applied directly to the media before autoclaving was effient in controlling lethal browning. On the other hand, the number and fresh weight of (Musa sp) var. ‘Yangambi’ was found to increase according to different buds spliting techniques, in which highest number of shoots and fresh weight was observed on quota splitted buds. BAP at concentration of 6mg/l was found to be the best treatment in terms of number of buds produced per explant. The interaction of BAP and IAA at the concentration of 6mg/l was also found to have significant effect on the fresh weight of shoots produced per explant. In rooting experiment, IBA at concentration of 2mg/l promoted highest number of roots produced per explant as well as the average shoot length compared with the rest of treatments. This study provides the insights for controling lethal browning and optimal concentration of auxins and cytokinins for micro propagation of var. ‘Yangambi’. It also directs the usefulness of bud spliting technique in increasing buds proliferation. Mlalila, N. MSc. (2013) Synthesis and characterization of methyltestosteroneloaded lipid nanoparticles for production of all-male tilapia cultures Abstract In recent years, all-male cultures of Nile tilapia are the most preferred mode of production, avoiding their early maturation and frequent spawning in mixed sex systems. All-male individuals achieve higher somatic growth rate and larger final size compared to females. Their economic advantages have led to the development of hormonal sex reversing strategies using methyltestosterone (MT). MT is a lipophilic drug administered to fish through impregnated feed in open water systems, a method that is complicated by poor uptake and leakages leading to environmental hazards to ecosystems and health effects to non-targeted animals. To control the challenges, novel delivery systems “nanotestes” were designed for oral administration hence expecting direct uptake from gastrointestinal tract (GIT) and long circulating effects in biological systems. The water-in-oil-in water nanotestes were formulated by double emulsion technique using 0.075% w/w stearic acid, 3% w/v PVA, 5% w/v lactose and 0.2% w/v chitosan. 118 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS The nanotestes were immediately spray dried into a top-bench spray dryer. The final nanotestes had particle size range of 120 and 170 nm and average encapsulation efficiency (EE) of 90%. Scanning electron microscopy (SEM) analysis showed that, the nanotestes were spherical in shape with smooth surfaces, results that were also confirmed by differential scanning calorimetry (DSC) to be in amorphous state. These nanotestes provided a promising carrier candidate for delivering MT. However, further studies are needed in order to establish stability and the cargo release profile under common conditions of use. The ultimate nanotestes should require modification of the prototype that allows single administration, slow and targeted delivery of MT to target tissues. Rubaka, C. MSc. (2013) Analysis of phytochemical and antibacterial activity of carissa spinarum and carica papaya crude extracts Abstract Carissa spinarum and Carica papaya are tropical medicinal plants containing bioactive substance essential for treatment of infectious diseases. The crude extracts of different parts of Carissa spinarum (C. spinarum) and Carica papaya (C. papaya) were screened for the presence of phytochemicals. The antibacterial activities of extracts alone and in combination were conducted to investigate the susceptibility of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) pathogens to plant extracts and improvement of efficacy upon their interaction. Susceptibility of pathogens to plant extracts was determined by disc diffusion sensitivity testing. Broth dilution method was used to determine minimum inhibition concentration (MIC) of individual and combined extracts and values of MICs were used to determine fraction inhibitory concentration and hence the type of interaction. The zone of inhibition for the pathogens tested ranged from 0.0 mm to 13.3 ± 1.5 mm. Carissa spinarum root methanolic extracts demonstrated the highest antibacterial activity compared to C. papaya leaves petroleum either extract (CPLP), C. papaya leaves ethanolic extract (CPLE), C. papaya root ethanolic extract (CPRE), C. papaya leaves methanolic extract (CPLM), C. spinarum leaves petroleum ether extract (CSLP), C. spinarum root ethanolic extract (CSRE), C. spinarum bark ethanolic extract(CSBE), C. papaya seed ethanolic extract(CPSE) and C. spinarum leaves methanolic extract(CSLM). NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 119 The MICs of extracts ranged from312 μg/ml to 5000 μg/ml. The MICs values of CPLP, CSLP, CPLE, CSRE, CPSE, CSBE, CPRE, CSLM, CPLM and CSRM against S.aureus were 625, 312, 312, 1250, 2500, 5000, 1250, 1250, 1250, 312μg/ml respectively while the values of MICs against E.coli were 1250, 2500, 1250, 312, 1250,5000,1250,2500,2500 and 312 μg/ml. Carissa spinarum bark extracts didn’t demonstrate any antibacterial activity however; it exhibited a synergistic effect with C. spinarum root ethanolic extracts against S.aureus. Another combination exhibiting synergy was C. papaya leaves methanolic extracts and C. spinarum leaves methanolic extracts. No synergy was exhibited by combined plant extracts against E. coli. Phytochemical screening revealed the presence of saponins, alkaloids, flavonoids, tannins and sterols. These studies indicate that antibacterial activity of different extracts of C. spinarum and C. papaya exhibited antibacterial activity against pathogens and the efficacy was improved through interaction. Carissa spinarum and C. papaya could be good sources of chemotherapeutic agents for the treatment of infectious diseases. Kavishe, S. MSc. (2013) Quality, microbial and chemical safety of banana alcoholic beverages case study: Arusha Tanzania Abstract Banana alcoholic beverages (BAB’s) processing is a rapid growing sector dominated by small and medium scale enterprises. It has played a major role in generating jobs, income and to some extent revenues to the government. The safety and quality of these beverages towards the public health are the main concern due to contamination of microorganisms and other contaminants. The purpose of this study was to analyze microbial quality and chemical safety of BAB’s produced in Arusha region. The data were gathered through survey which involved interviewing 12 production attendants and laboratory analysis. The results from the study showed that 11 (91.7%) production attendants don’t have any knowledge on good hygienic practices and only 4 (33.3%) producers treat water before processing. Filling of BAB’s is done manually by 9 (75%) processors and 10 production attendants (83.3%) use normal soap instead of disinfectant. From laboratory analysis, the results of total bacteria count were between 1.07 × 104 - 2.53 × 104 cfu/mL and 0.92 × 104 - 2.54 × 104 cfu/mL for pre-bottled and bottled BAB’s respectively, indicating poor hygienic practices during processing of BAB’s. Coliforms ranged from 8.0 × 101 to 3.80 120 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS × 103 MPN/mL for pre-bottled BAB’s and 6.0 × 101 to 2.0 × 1 03 MPN/mL for bottled BAB’s respectively. Tanzania Bureau of Standards requires no coliforms in BAB’s. Also, presence of Escherichia coli was confirmed in 5 out of 12 samples indicating the possibility of using water contaminated with fecal matter during processing. Furthermore, no Salmonella contamination was detected in the all samples which comply with the standards for the non-cereal based alcoholic beverages stipulated by Tanzania Bureau of Standards (TBS). The levels of methanol were within the acceptable limits signifying that no poor manufacturing practices or adulteration in BAB’s. Insignificant variation in ethanol level was observed in pre-bottled and bottled BAB’s respectively which were within acceptable level (10% v/v) for non- cereal based alcoholic beverages. Understanding microbial contamination and physicochemical changes which occur during processing of BAB’s is essential in improving the microbial and chemical, quality and safety of BAB’s. Masaki, S. S. MSc. (2013) Prevalence of type 2 diabates amongst selected Maasai ethnic group: the role of rural to urban migration Abstract The purpose of this study was to investigate whether change in lifestyle from rural to urban environment will change the prevalence Type 2 diabetes (T2D) amongst Maasai. Method: A cross-sectional investigation of 724 Tanzanian Maasai including 374 rural (male 142, female 232) and 350 urban (male 193, female 157) participants aged 18-75years was conducted. Anthropometric measures (i.e., body mass index (BMI); waist to hip ratio (WHR), blood glucose, serum lipids, self-reported dietary practice, lifestyle (i.e., alcohol/tobacco use) and physical activity patterns were assessed. Results: T2D is defined as occurring when not enough insulin is produced or the insulin that is made by the body does not exert the usual effects on body cells (WHO, 1999). In this study prevalence of T2D was 22.9% (n=80) in urban and 9.9% (n=37) in rural areas. The urban T2D was significantly (p<0.05) positively correlated with obesity markers as denoted by BMI, weight, waist-circumference, hip circumference and WHR. Urban hypertension was also significantly (p<0.05) correlated with other well established T2D risk factors such as systolic blood pressure and age. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 121 Age-dependent change in the prevalence of T2D was typical of what is reported in many urban centres. Prevalence increased with age and was highest at 60years and above. Gender differences were only apparent between 40-59 years where the prevalence in males was significantly higher than females. A significantly higher (p<0.05) proportion of urban Maasai were overweight, consumed more meals per day, took alcohol, and participated in less physical activities. For rural Maasai, the fasting blood sugar (FBS) was significantly (p<0.05) positively correlated with the obesity markers (BMI, weight, hip circumference and waist circumference). In contrast to urban FBS, age was significantly negatively correlated with FBS. Conclusion: This study shows that the rural to urban migration amongst the Maasai ethic tribe exposes them to higher risk for T2D. Obesity, age, and systolic blood pressure were found to be the major risk factors in urban Maasai contributing to a shift towards higher T2D. Xavery, D. K. MSc. (2013) Developing a tissue culture protocol for regenerating the threatened African sandalwood (osyris lanceolata) Abstract African sandalwood is indigenous to eastern and southern African regions and its heartwood oil is highly sought leading to over exploitation of the tree, and a threat to the survival of the species. The plant has poor seed germination and its vegetative propagation using root suckers is slow and takes time for replacement. I n o r d e r to overcome challenge of overexploitation there has been introduced a propagation method namely in vitro. The method is a potential viable means for regeneration of the plant. This study was set out to explore the capacity of the plant explants to propagate on artificial basal medium. Concentrations of sodium hypochlorite at a concentration of 20%, 30% and 40% were tested and explants were either treated with ascorbic acid. This study has established a simple sanitation protocol which uses 20% and 40% of sodium hypochloride bleach solution for leaf and nodal segments explants respectively. The study has demonstrated that for the first time the highest number of African sandalwood callus from leaf and nodal explants was developed when Murashige and Skoog basal medium supplemented with 5micromoles of Benzylaminopurine. The study recommends the protocol developed as a benchmark for obtaining viable and clean explants material for other woody plants species for use in tissue culture. 122 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Furthermore, the study recommends the use of both nodal segments and leaf explants materials of the African sandalwood for establishment of tissue culture. Ismail, S. MSc. (2013) Students’ conceptual understanding of computational round-off arithmetic in Tanzanian schools: A case study of high school students in Hai and Moshi Urban Districts Abstract Integration of the calculator in classroom mathematical instructions has brought about challenges such as students’ misconceptions of mathematical ideas owing to its computing limitations. This problem has been an opportunity for researchers to assess students’ awareness of its computing nature. Moreover, many research studies have been conducted to explore students’ understanding of these calculating machines but such studies have not been comprehensively done in Tanzania. The main objective of this dissertation was to examine students’ conceptual understanding of computational round-off arithmetic in Tanzanian schools. In particular, the study was aimed at exploring mathematics students’ awareness of calculator computing limitations when they use it to solve mathematical problems. The research study used a survey study questionnaire to collect data from 147 students of 4 high schools selected in the Hai and Moshi Urban Districts in the Kilimanjaro Region. The data was analyzed using Computer Statistical Package (Excel 2007) and Normal Distribution Calculator (Casio fx � 991M S ) . The study reveals that the majority of mathematics students do know the computing limitations of calculators. It also shows that there is no association between study year and mathematics students’ awareness of calculator round-off arithmetic and its impact in computations. The study discloses that male and female mathematics students do not differ significantly in the manner they view the impact of calculator arithmetic on computations when solving mathematical problems. Thus, the study findings disclose the importance of knowing the calculator computing nature when solving mathematical problems to get rid of unnecessary students’ misconceptions during mathematics learning. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 123 Kahuru, S. MSc. (2013) Mathematical model for water resource planning in irrigation: The case of wine grape farming in Dodoma Region Abstract Optimal cropping pattern in vineyard irrigated agriculture is one of the essential tasks for obtaining the optimal irrigation water resources of the command. This can be achieved by developing a Mathematical optimization model which plays a vital role in planning and management of irrigation water resource. An application of linear optimization methodology was used as a solution to a problem in the study area of Chinangali irrigation farmland with the cultivated command area of 120 hectares located in the Dodoma region of Tanzania. The data on crops weather, soils, bore holes supply, and cost of cultivation pertaining to the study area were collected from various Government offices, institutions and personal contact from the grapevine farmers of the study area. In this study the mathematical optimization model was formulated for optimal utilization of water and land resources and was solved using MATLAB. Greater emphasis was being placed on the need to improve the efficiency in use of the minimum available water resources for grapevine production. The estimation of the crop water requirement was done with the aid of the FAO Penman-Monteith Equation for the optimal planning of cropping pattern, to minimize irrigation water resource for the study area. The results from the Penman’s Monteith equation FAO paper 56 show that the annual crop water requirements for grapevine varieties: Cabernet sauvignon, Chardonnay, Chenin blanc and Riesling were; 520.7 mm, 746.6 mm, 1066.4 mm and 1235.8 mm respectively. The optimized results from the MATLAB analysis show that when the decision of planting 27.97 hectares of Cabernet sauvignon, 14.18 hectares of Chardonnay, 21.39 hectares of Chenin blanc and 56.14 hectares of Riesling 16 470.40 m3 per annum of water can be saved. For that reason it was suggested that 1 173 359.60 m3 per annum should be released to the vineyards of the study area for optimum irrigation planning against the 1 189 830 m3 per annum supplied presently. 124 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS SCINTIFIC ARTICLES PUBLISHED FROM COSTECH FUNDED RESEARCH ROJECTS R. R. Minja1, J. Ngailo2, N. Mwalukasa2 and S. Kyando2 (;2014); Effects of Phosphate Rock on Phosphorus Availability for Vegetable Crops in Cropping Sequence International Journal of Plant & Soil Science 3(5): 434-447, Article no. IJPSS.2014.001SCIENCEDOMAIN international www.sciencedomain.or 1Mikocheni Agricultural Research Institute, P. O. Box 6226, Dar es Salaam, Tanzania. 2Uyole Agricultural Research Institute, P. O. Box Mbeya, Tanzania. Abstract This study was conducted to investigate the effects of cropping sequence and Minjingu Phosphate Rock (MPR) on soil phosphorus (P) availability and yield of selected vegetable crops. The study involved a researcher managed on farm field experiment conducted in Mbeya, Tanzania using a split plot design with three replications. Crop sequence (i) Cabbage - tomato - maize (ii) Maize - tomato -cabbage (iii) Cabbage - cabbage - maize (iv) Maize - tomato – maize, constituted the main plots. The P sources (i) control (no fertilizer), (ii) MPR, (iii) compost + MPR, (iv) crotalaria green manure + MPR (v) NPK (standard), were sub plots. The experiment was repeated three times on the same plots. In the fourth season, bean was planted in all plots without fertilizer to capture residual nutrients. Results indicated more percent increase in soil available P in plots where cabbage was included in the sequence (207.9%) as compared to when it was ommited (85.39%). Compost + MPR treatment significantly increased soil pH from 5.8 to 6.33 while in NPK treated plots it decreased to 5.49. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 125 After three seasons there was higher residual P in MPR treated plots (33.33 mg/kg) as compared with NPK (27.65mg/kg). Plots treated with NPK produced the highest maize yield while plots treated with MPR alone or combined with compost or crotalaria produced significantly (P ≤ .01) higher cabbage and tomato yield especially during the first and second seasons. Bean pod yield was higher in MPR treated plots. Therefore, the use of PR and including cabbage in crop rotations should be encouraged in organic vegetable farming systems to restore P on phosphorus deficient soils. Zacharia Katambara1, Frederick C. Kahimba2, Winfred B. Mbungu2, Paul Reuben2, Muyenjwa. Maugo3, Fikiri D. Mhenga2, and Henry F. Mahoo2 . Optimizing System of Rice Intensification Parameters Using Aquacrop Model for Increasing Water Productivity and Water Use Efficiency on Rice Production in Tanzania. Journal of Agriculture and Sustainability Volume 4, Number 2, 2013, 235-244 1. Department of Civil Engineering, Mbeya University of Science and Technology, P.O. Box 131, Mbeya, Tanzania 2. Department of Agricultural Engineering and Land Planning, Sokoine University of Agriculture, P.O. Box 3003 Morogoro, Tanzania 3. Ministry of Agriculture, Food Security and Cooperatives, P.O. Box 9192, Dar es Salaam, Tanzania Abstract Producing more rice while using less water is among the calls in water scarce regions so as to feed the growing population and cope with the changing climate. Among the suitable techniques towards this achievement is the use of system of rice intensification (SRI), which has been reported as an approach that uses less water and has high water productivity and water use efficiency. 126 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Despite its promising results, the use of SRI practice in Tanzania is limited due to less knowledge with regard to transplanting age, plant spacing, and minimum soil moisture to be allowed for irrigation, and alternate wetting and drying interval for various geographical locations. The AquaCrop crop water productivity model, which is capable of simulating crop water requirements and yield for a given parameter set, was used to identify suitable SRI parameters for Mkindo area in Morogoro Region, Tanzania. Using no stress condition on soil fertility, plant spacings ranging from 5 cm to 50 cm were evaluated. Results suggest that the yield and biomass produced per ha increase with decreasing spacing from 50 cm to 20 cm. Preliminary field results suggest that the optimum spacing is round 25 cm. However, the model structure does not take into consideration number of tillers produced. As such, the study calls for incorporation of the tillering processes into Aqua Crop model. Zacharia Katambara1*, Frederick C. Kahimba2, Henry F. Mahoo2, Winfred B. Mbungu2, Fikiri Mhenga2, Paul Reuben2, Muyenjwa Maugo3, Anthony Nyarubamba3 . Adopting the system of rice intensification (SRI) in Tanzania: A review Agricultural Sciences 4 (2013) 369-375 1. Department of Civil Engineering, Mbeya University of Science and Technology, Mbeya, Tanzania; *Corresponding Author: [email protected], [email protected] 2. Department of Agricultural Engineering and Land Planning, Sokoine University of Agriculture, Morogoro, Tanzania 3. Directorate of Irrigation, Ministry of Agriculture, Food Security, and Cooperatives, Morogoro, Tanzania Abstract The demand of water for irrigation purposes in Tanzania outstrips the amount of water available for irrigation and other demands. On the other hand, the demand for more food to feed the growing population is increasing, calling for the need to have technologies and farming practices that ensure more food production while minimizing water uses. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 127 Rice is among cereal crops grown in Tanzania, and it can assist in meeting the food demand for the nation. Majority of rice producers in Tanzania and Sub-Saharan Africa (SSA) are subsistence farmers and they practice continuous flooding, a technique that requires much water. In addition to using large amounts of water, the conventional practices of growing paddy using local varieties transplanting process are implemented when seedlings are more than 21 days old, and 3 - 4 seedlings are transplanted in one hole. This practice results in low yields, and low water productivity and water use efficiency. The system of rice in- tensification (SRI) on the other hand, is a promising new practice of growing paddy rice that has proven to be very effective in saving water and increasing rice yields in many parts of the world. SRI practice is spreading fast and it has been adopted in many countries. The SRI practice has been introduced in Tanzania during the last 3 years as such it is not widely practiced. This paper reviews SRI practice at global, regional and country (Tanzania) level, and evaluates the challenges, opportunities and implications for its adoption in Tanzania. Knowledge gaps at each level have been identified and discussed as well as suggestions for researchable areas. Kahimba, F.C.1, Kombe, E.E.2, and Mahoo, H.F.1. The Potential of System of Rice Intensification (SRI) to Increase Rice Productivity: a Case of Mkindo Irrigation Scheme in Morogoro Region, Tanzania. Tanzania Journal of Agricultural Sciences (2014) Vol. 12 No. 2, 10-19 1. Department of Agricultural Engineering and Land Planning, Sokoine University of Agriculture, P. O. Box 3003, Morogoro, TANZANIA 2. Mwanza Zonal Irrigation and Technical Services Unit, Ministry of Agriculture, Food Security and Cooperatives (MAFC), Mwanza, TANZANIA Abstract Rice plays a critical role in ensuring food security in developing countries. For majority of the world’s small-scale farmers who live in Asia and sub-Saharan Africa, rice is a major source of calories and the single largest source of income. However, increases in rice production are now lagging behind population growth, compounded by effects of climate change and variability. 128 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS The system of rice intensification (SRI) developed in Madagascar, is a system approach to increase rice productivity through proper management of fewer inputs such as irrigation water and seeds. This study was therefore designed to evaluate the performance of SRI in Mvomero district in Morogoro region, Tanzania by implementing farmer field school (FFS) pilot trials of SRI operated by farmers alongside on-station scientific experiments in Mkindo Irrigation scheme. The experiments were conducted for two consecutive years during the wet season (March- July 2011) and dry season (September 2011- January 2012). One rice variety TXD 306 (SARO) was planted on plots in a randomized complete block design (RCBD) with five treatments based on two water application regimes of flooding and alternate wetting and drying (AWD), while the effects of transplanting age of seedlings and plant spacing (in cm) of 20x20 for T1 and T2, 25x25 for T3, 30x30 for T4, and 40x40 for T5 were evaluated. The plant height, root depth, tillerig, biomass and grain yields, irrigation water use, and wetting and drying intervals were evaluated and results were statistically analyzed using GENSTAT software. Highest grain yield was achieved in 25x25 (T3) and 30x30 (T4) SRI spacing. Under the SRI practice, 62.51%, 63.64%, 64.67%, and 64.07% water savings were noticed for T2, T3, T4 and T5, respectively, compared to the control (T1). SRI practice for planting space of 25x25 to 30x30 cm, wetting and drying interval of three days, and younger seedling of 8-12 days are recommended as good combinations for SRI practice in Mkindo area, Morogoro region. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 129 P Y Kavana and V A M Kakengi* Availability of pasture for domestic and wild herbivores in grazing land of Mpanda Tanzania. Livestock Research for Rural Development 26 (2) 2014 Mahale-Gombe Wildlife Research Centre, P.O. Box 1053, Kigoma, Tanzania [email protected] * Tanzania Wildlife Research Institute, P.O. Box 661, Arusha, Tanzania [email protected] Abstract A study was conducted to assess feed resource base for domestic and wild herbivores in grazing lands of Mpanda district. Forage samples were taken from various grazing lands in wet and dry seasons. Results indicated that feed resources for domestic and wild herbivores in Mpanda district is based on miombo woodlands vegetations. Various plant species were identified that indicate plants diversity in grazing lands. However, plant species edible to domestic and wild herbivores were 42.9% of the available plant species population in grazing lands. Furthermore, the edible plant species were confined to 9 plant families out of 39 plant families documented in the study area. This implies that feed resource base for domestic and wild herbivores in Mpanda district is narrow. Therefore effort through establishment of proper land use plans in villages is required to ensure sustainability of domestic and wild herbivores as well as feed resources in Mpanda district. 130 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 1Christopher L. Materu, 2Seguni Z. Kombo and 3Linus Masumbuko. Ant species diversity and composition at Mlingano mango orchard in Tanzania. Research Journal of Agricultural and Environmental Sciences Volume-1, Issue-2, Nov-Dec-2014, Res.J.Agr.Env.Sci. www.rjaes.com. 1,2,3 P O Box 6226, Mikocheni Agricultural Research Institute Tanzania *[email protected]; [email protected], [email protected] Abstract Ants are major decomposers and they are sensitive to any human influence in our environment. Presence or absence of ants in cropland habitat is resulted from alteration of forest habitat into cropland. Ant’s species diversity in mango orchard was considered as an important biodiversity indicator due to alteration of the primary forest into cropland habitat. Ants were sampled from mango trees searching species of ants from tree bases to 2M high. Dental rolls with 10% sugar as attractant bait were also used to attract ground preference of ant species. Attractant bait was placed on a tree base for 30 minutes and thereafter inspected for data collection. This study has revealed that ant species composition in mango orchard varies according to cropping system. Dominance of species was Crematogaster followed by Pheidole megacephala and the least was Oecopyhlla longinoda ants. Comparison of species composition revealed that there were significant differences. Species with low representative individuals were considered as extinct or rare species. The intended study was carried out at Mlingano mango orchard with the aim to determine the ant’s species diversity and composition. The results from this work will help in developing sustainable biodiversity conservation programmes as well as for future research. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 131 Christopher L. Materu1*; Zuberi Seguni2; Evarista, E. Shao, Beatrice M. Mruma, Andrew J. Ngereza2. Assessing the Impact of Integrated Pest Management Programme for Management of Fruit Flies (Diptera: Tephritidae) On Mango Orchards in Tanzania. Research Journal of Agricultural and Environmental Sciences Volume-1, Issue-2, Nov-Dec-2014, Res.J.Agr.Env.Sci. www.rjaes.com 1. Christopher L. Materu P. O. Box 6226, Mikocheni Agricultural Research Institute Tanzania 2. Zuberi K. Seguni P, O Box 6226, Mikocheni Agricultural Research Institute Tanzania 3. Andrew J. Ngereza P. O. Box 6226, Mikocheni Agricultural Research Institute Tanzania 4. Evarista E. Shao P. O. Box 6226, Mikocheni Agricultural Research Institute Tanzania 5. Beatrice M. Mruma P. O. Box 6226, Mikocheni Agricultural Research Institute Tanzania Abstract Fruit flies (Diptera: Tephritidae) are a major threat to the vegetables and mango industry in the world. The female flies prick and lay eggs under the skin of the fruits. The eggs hatch and release the larvae which present three immature stages before reaching adult stages. The first two larval stages occur in plant tissues and cause the premature fall of the fruits. The invasive fruit fly Bactrocera invadens was first detected in Tanzania in 2004. Direct damage due to B. invadens attack in African countries varies between 3080%. Therefore, the objective of this study was to assess the impact different Integrated Pest Management (IPM) techniques on suppression of mango fruit flies in Muheza district. Improvised twenty traps baited with methyl eugenol hanged 2m above the soil surface was used to trap the flies. Traps were inspected every after 7days and were recharged every after 60 days. 132 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS Traps were left on the same position throughout the entire period of this study. Furthermore, application of Mazoferm bait spray every after two weeks followed by regular fruit collection on weekly basis were considered as full IPM African Weaver Ant (AWA) enhancement was considered as biological control agent. Fruits from different treatment were collected during development stage, green stage and at fully ripening stage. Fruits samples were randomly collected within mango orchards except from weaver ant where by fruits collected were those observed foraged with weaver ants. A total of 2,848 fruit flies belonging to the genera Bactrocera were captured during the study period. B. invadens was the dominant species recorded in all traps during the main fruiting periods of September to December 2013. The effect of Different IPM showed significant differences between sampling dates. Similarly comparison of fruits damage from different treatments showed significant differences (P=0.05). These findings demonstrate that fruits from weaver ants showed AWA significantly reduces B.invadens and needs to be conserved. Fruit fly IPM in the study area should be targeted at different stages of fruit development using singly or combined IPM technologies. Samwel Mchele Limbu; Amon Paul Shoko; Hieromin Amon Lamtane; Evantuce Dominick Shirima; Mary Alphonce Kishe-Machumu; Huruma Fredrick Mgana; Yunus Daud Mgaya. Effect of initial stocking size of the predatory African sharptooth catfish (Clarias gariepinus) on recruits, growth performance, survival and yield of mixed-sex Nile tilapia (Oreochromis niloticus) in concrete tank culture system. Int Aquat Res DOI 10.1007/s40071-014-0093-3, published online: 21st January 2015 Abstract Prolific breeding and production of high percentage of recruits are main problems in mixed-sex Nile tilapia (Oreochromis niloticus) culture in earthen ponds. The current study assessed the efficiency of different sizes of African sharptooth catfish (Clarias gariepinus) in controlling recruitment in mixed-sex Nile tilapia cultured in tanks and its effect on growth performance, percentage survival and yield. NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS 133 Three treatments consisting of triplicate ponds stocked with mixed-sex Nile tilapia in monoculture and in polyculture with large (62.50 ± 3.26 g) and small (40.00 ± 2.68 g) African sharptooth catfish were fed on a 297.50 g kg-1 crude protein diet for 126 days. Results showed that, the number of recruits was significantly lower in larger African Sharptooth catfish predator than smaller ones and monoculture of Nile tilapia. Polyculture with larger African sharptooth catfish resulted in significantly higher growth performance of Nile tilapia. Large African sharptooth catfish in polyculture reduced the amount of small, low-value recruits, while the yield of large and high value Nile tilapia was increased. This study revealed that fish farmers can reduce prolific breeding, obtain higher growth performance and produce larger size of marketable Nile tilapia by predominantly stocking ponds with large African sharptooth catfish predator of at least 60g. 134 NFAST FUNDED RESEARCH PROJECTS 2012-2015 A BOOK OF ABSTRACTS