CONCLUSION about the primary toxicological assessment of liquid organic fertilizer

CONCLUSION
about the primary toxicological assessment of liquid organic fertilizer
(sample 1) produced by “Bio Organic Georgia” Ltd.
The designation of the presented sample of the liquid-organic fertilizer (sample 1) is root and
rootless nutrition of the plant at any stage of its development.
According to the presented materials (laboratory analyses), the content of the study fertilizer is as
follows: 3,5% of ammonia acid in total product, 11,55% of humic acid in “Organica”, 5,0% of total
nitrogen, 0,35% of phosphor, 8,5% of potassium, 0,8% of iron, 10,2% of calcium, 4,5% of
magnesium and other organic and mineral substances, macro and micro elements.
The aggregate state of the study material is as follows: dark brown transparent liquid with specific
odour, with the number of hydrogen ions of 7,9. The sample fertilizer is packed in a 0,5-litre
polyethylene vessel.
The toxicological study of the liquid-organic fertilizer was accomplished by the following
experiments to identify the following:
(a) Toxicity of the matter in case of getting in the gastrointestinal tract;
(b) Toxicity of the matter if applied to the human skin or eye mucosa.
The experimental studies were undertaken by using standard methods.
The model of biological impact of the study fertilizer on the body was created by one-time oral
administration (with intervals, during the day) (to rats, with doses > 10 000 mg/kg), skin application
(rabbits, rats) and mucus membrane (rabbits) in terms of native impact (application). The general
toxicity was evaluated based on integral indicators, such as animal behavior, its general state, time,
severity, course and outcome of intoxication, eating and drinking, dynamics of body mass increase,
visual state of skin and fur cover, macroscopic photos of the internal organs of fallen animals, etc.
The accomplished studies revealed the following:
1. A single administration of high doses (>10 000 mg/kg) of the study liquid fertilizer (sample
1) to the animal’s gastrointestinal tract does not cause severe intoxication or falling of the
animal. Accordingly, no main toxicological parameter, the average lethal dose LD50 could be
calculated. Following the administered maximum dose, the given parameter LD50>35000
mg/kg. Based on these data, the study fertilizer may be attributed to the group of low-toxic
substances, with the danger class U. This indicates that in terms of normal application of the
study fertilizer, no danger of severe intoxication for those in contact with the fertilizer is
expected (WHO, Classification 2004).
2. A single native application of the study liquid fertilizer (sample 1) to skin (rabbits, rats) and
mucus membrane (rabbits) does not cause local irritation or skin-resorption effect.
So, the liquid fertilizer (sample 1), as per the accomplished studies, can be used according to its
intended purpose.
In addition, during the production and usage of the fertilizer, the personal protection equipment
(PPI) should be used for the labor safety purposes.
Javakhadze R., Academic Doctor, Director General of N. Makhviladze State Scientific-Research
Institute of Labor Medicine and Ecology (signed)
Gvineria I., Academic Doctor, Expert, Head of the Department of Preventive Toxicology of the
Institute (signed)
12.08.2010