Legend News BioLegend Acquires Covance Antibodies October 2014
Transcription
Legend News BioLegend Acquires Covance Antibodies October 2014
Legend News October 2014 BioLegend Acquires Covance Antibodies BioLegend has acquired Covance’s antibody products business unit, located in Dedham, Massachusetts. This unit includes R&D and manufacturing of antibodies and research reagents for Neuroscience, Immunopathology, Cell Biology, Epitope Tags, and Immunohistochemistry (IHC) Detection. These products, including In Vitro Diagnostic (IVD) antibodies for IHC, complement and enhance the current selection of BioLegend research reagents. Former Covance antibody customers can expect the same superior customer support and exceptional value with these products as existing BioLegend customers. As neuroinflammation and neurodegeneration become increasingly important topics, BioLegend is committed to the advancement of discovery in neuroscience. With the addition of a world-class R&D team and over 600 antibody products for emerging and important targets in this field, BioLegend is positioned to become a leader in neuroscience and immunology research. Staining of pyroglutamyl E3 Amyloid Beta antibody on formalin-fixed paraffin-embedded human Alzheimer’s diseased brain tissue. Learn more about our acquisition of Covance Antibodies at: biolegend.com/covance_antibody_acquisition View our new neuroscience reagents at: antibody.biolegend.com BioLegend Blog: Insight, Tips, and Humor Come and Meet Us! October 7-10 Ocular Immunity, Autoimmunity, & Inflammation Ft. Lauderdale, FL October 10-14 ICCS 2014 Conference Seattle, WA Western Blot showing reactivity of APP751 (full-length amyloid precursor protein) using 4G8 antibody. Lane 1: Molecular weight marker Lane 2: 50 ng APP751 protein. October 23-25 Society for Leukocyte Biology 2014 Salt Lake City, UT Our blog covers a diverse set of topics ranging from graduate school advice to scientific history to technical flow cytometry advice. Expand your horizons at: biolegend.com/blog. Recent posts: • The Placebo Effect • Pricing Pharmaceuticals, Part 2 • Lab Tips 1: Cutting Lab Costs Through Re-engineering 9727 Pacific Heights Blvd.|San Diego, CA 92121 1.877.246.5343 (877-BIOLEGEND) International: 1.858.768.5800 biolegend.com 02-0001-31 BioLegend | Star Products PE/Dazzle™ 594 Conjugates PE/Dazzle™ 594 is BioLegend’s newest fluor and offers outstanding brightness, pricing, and multicolor flow cytometry flexibility. This fluor is equivalent to PE/Texas Red®, PE-CF594, PE-eFluor® 610, and ECD, and is optimally stimulated by the 561 nm yellow/green laser. We will continue to support this fluor with a continuous release of conjugates, and you can utilize the promotion on page (3) to save even more. PE/Dazzle™ 594 anti-human IL-17A Antibody PE/Dazzle™ 594 anti-mouse/human KLRG1 (MAFA) Antibody C57BL/6 mouse splenocytes were stained with NK1.1 FITC and KLRG1 (clone 2F1/KLRG1) PE/Dazzle™ 594. PMA + Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 FITC, fixed, permeabilized, and then stained with IL-17A (clone BL168) PE/Dazzle™ 594 (left) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (right). Learn more about PE/Dazzle™ 594 at: biolegend.com/PE-Dazzle594 Description Size Cat. No. PE/Dazzle™ 594 anti-human IL-17A Antibody 25 tests | 100 tests 512335 | 512336 PE/Dazzle™ 594 anti-mouse/human KLRG1 (MAFA) Antibody 25 μg | 100 μg 138423 | 138424 Microscopy Fluors We are proud to support a variety of reagents for microscopy imaging of cells and tissue samples. Our reagents include antibody conjugates to bright, photostable fluors like Brilliant Violet 421™, Brilliant Violet 510™, and the Alexa Fluor® dyes. We also provide cell tracking dyes and nuclear counterstains. We will continue to release new microscopy products as we discover innovative technologies. Alexa Fluor® 594 anti-human Granzyme A Human PBMCs were fixed, permeabilized, and blocked. Next, cells were stained with Granzyme A (clone CB9) Alexa Fluor® 594 (red), washed, then co-stained with CD8 (clone HIT8a) Alexa Fluor® 488 (yellow) and CD56 (clone HCD56) Alexa Fluor® 647 (green). Nuclei were counterstained with DAPI (blue). The image was captured by a 60X objective. Download the Microscopy flyer from our literature page: biolegend.com/literature Brilliant Violet 510™ anti-human CD11c Human peripheral blood mononuclear cell derived dendritic cells were fixed and then stained with CD11c (clone 3.9) Brilliant Violet 510™ (yellow). Nuclei were counterstained with DAPI (blue). The image was captured by a 40X objective. Description Size Cat. No. Alexa Fluor® 594 anti-human Granzyme A Antibody 100 μg 507218 Brilliant Violet 510™ anti-human CD11c Antibody 25 tests | 100 tests 301633 | 301634 BioLegend | Purple Perks DAZZLE Promotion ELISA Promotion Get a 30% discount on all PE/Dazzle™ 594 conjugates. Use code: DAZZLE-14. For US and Canada end-users only. Not available for resellers or distributors. It’s time for ELISAs! Get a 40% discount on all LEGEND MAX™ kits and ELISA MAX™ sets until Nov. 30, 2014. Use code: ELISA40-14 Expires December 31, 2014. Valid for US and Canada customers only. Learn more at biolegend.com/promotions Learn more at biolegend.com/promotions Microgram (μg) Sizing We are always looking to provide product flexibility and cost savings so you’re not breaking the bank for our reagents. As such, we are proud to announce new 100 µg sizing for several popular and well-established anti-human clones conjugated to common fluors like PE, FITC, Alexa Fluor® 488, Alexa Fluor® 647, and APC. Purchasing antibody conjugates in µg sizing allows you to: • Know exactly how much antibody you are receiving without having to contact technical support. • Get lot-to-lot concentration consistency. • Save dramatically on costs and reagent when you titrate the antibody. See the full product listing at: biolegend.com/microgram_sizing Innate Lymphoid Cells Webpage Innate lymphoid cells (ILCs) are new, intriguing cell populations involved with tissue development, remodeling, and inflammation. However, classifying these cells has been difficult, due to a variety of naming conventions. Now you can use our new webpage to learn about their phenotyping, classification, and development. Check out our new ILC webpage at: biolegend.com/ilc BioLegend | New Products Released this Month Human MaxPar® Ready Antibodies Human Recombinant Proteins Description Size Cat. No. Description Size Cat. No. Purified anti-human CD206 (MMR) (MaxPar® Ready) 100 μg 321127 Recombinant Human CCL4 (MIP-1β) (carrier-free) 10 μg | 25 μg | 100 μg 554702 | 554704 | 554706 Recombinant Human FAS (TNFRSF6)-Fc Chimera (carrier-free) 10 μg | 25 μg 100 μg | 500 μg 555002 | 555004 555006 | 555008 Recombinant Human Granzyme B (carrier-free) 10 μg | 25 μg 554902 | 554904 Recombinant Human IGF-BP7 (carrier-free) 10 μg | 25 μg 100 μg | 500 μg 555102 | 555104 555106 | 555108 Recombinant Human LDLR (carrier-free) 25 μg | 100 μg 555304 | 555306 Description Size Cat. No. Recombinant Mouse B7.1 (CD80)-Fc Chimera (carrier-free) 25 μg | 100 μg 555404 | 555406 Recombinant Mouse CCL4 (MIP-1β) (carrier-free) 10 μg | 25 μg | 100 μg 554602 | 554604 | 554606 Recombinant Mouse Granzyme B (carrier-free) 10 μg | 25 μg 554802 | 554804 Recombinant Mouse PCSK9 (carrier-free) 10 μg | 25 μg | 100 μg 555502 | 555504 | 555506 Mouse MaxPar® Ready Antibodies Description Size Cat. No. Purified anti-mouse CD45.1 (MaxPar® Ready) 100 μg 110745 Purified anti-mouse TER-119/Erythroid Cells (MaxPar® Ready) 100 μg 116241 Purified anti-mouse CD41 (MaxPar® Ready) 100 μg 133919 Size Cat. No. ELISA Description Biotin anti-human IL-2 50 μg 517605 Microscopy Description Size Cat. No. Alexa Fluor® 594 anti-human Granzyme A 100 μg 507218 Brilliant Violet 510™ anti-human CD11c* 100 tests 301634 *Also useful for flow cytometry Mouse Recombinant Proteins Legend News October 2014 BioLegend | New Products Released this Month (cont.) Mouse Flow Cytometry Human Flow Cytometry Description Size Cat. No. Description Size Cat. No. Description Size Cat. No. Alexa Fluor® 488 anti-human CD56 (NCAM) 25 tests 100 tests 362517 362518 PE anti-human CD3 100 μg 300456 APC anti-mouse TCR Vβ6 25 μg 100 μg 140005 140006 Alexa Fluor® 647 anti-human CD45 100 μg 304056 APC anti-mouse/rat XCR1 25 μg 100 μg 148205 148206 Alexa Fluor® 647 anti-human CD56 (NCAM) 25 tests 100 tests 362513 362514 Alexa Fluor® 700 anti-human CD56 (NCAM) PE anti-human CD4 100 μg 300550 PE anti-human CD8a 100 μg 301064 PE anti-human CD16 100 μg 302056 Brilliant Violet 510™ anti-mouse CD41 50 μg 133923 PE anti-human CD25 100 μg 356134 PE anti-mouse CD282 (TLR2) 25 μg 148603 PE anti-human CD27 100 μg 302842 PE/Cy7 anti-mouse CD133 25 μg 100 μg 141209 141210 PE/Dazzle™ 594 anti-mouse CD45.2 25 μg 100 μg 109845 109846 PE/Dazzle™ 594 anti-mouse CD80 25 μg 100 μg 104737 104738 PE/Dazzle™ 594 anti-mouse F4/80 25 μg 100 μg 123145 123146 25 tests 100 tests 362521 362522 Alexa Fluor® 700 anti-human Ki-67 25 tests 100 tests 350529 350530 PE anti-human CD45 100 μg 304058 APC anti-human CD3 100 μg 300458 PE anti-human CD45RO 100 μg 304244 APC anti-human CD4 100 μg 300552 PE anti-human CD62L 100 μg 304840 100 μg 305438 APC anti-human CD11b 100 μg 301350 PE anti-human CD86 APC anti-human CD127 (IL-7Rα) 100 μg 351342 PE anti-human CD127 (IL-7Rα) 100 μg 351340 APC anti-human CD195 (CCR5) 25 tests 100 tests 359121 359122 PE/Cy5 anti-human CD56 (NCAM) 25 tests 100 tests 362515 362516 PE/Dazzle™ 594 anti-mouse/human KLRG1 (MAFA) 25 μg 100 μg 138423 138424 Brilliant Violet 421™ anti-human CD31 100 tests 303124 PE/Cy7 anti-human CD26 25 tests 100 tests 302713 302714 PE/Dazzle™ 594 anti-mouse Ly-6A/E (Sca-1) 25 μg 100 μg 108137 108138 Brilliant Violet 421™ anti-human CD49d 25 tests 100 tests 304321 304322 PE/Cy7 anti-human CD85k (ILT3) 25 tests 100 tests 333011 333012 Purified anti-mouse/rat CD42d 50 μg 500 μg 148501 148502 Brilliant Violet 421™ anti-human CD360 (IL-21R) 25 tests 100 tests 359509 359510 PE/Dazzle™ 594 anti-human CD15 (SSEA-1) 25 tests 100 tests 323037 323038 Purified anti-mouse P2X7R 100 μg 148702 Brilliant Violet 510™ anti-human CD10 100 tests 312220 Purified anti-mouse/human SEMA4A 100 μg 148402 Brilliant Violet 510™ anti-human CD38 100 tests 356612 Purified anti-mouse/rat XCR1 100 μg 148202 Brilliant Violet 510™ anti-human CD40 25 tests 100 tests 334329 334330 Brilliant Violet 510™ anti-human CD197 (CCR7) 100 tests 353232 Description Size Cat. No. Brilliant Violet 570™ anti-human IFN-γ 100 tests 502534 Alexa Fluor® 594 Mouse IgG1, κ isotype ctrl 100 μg 400174 Brilliant Violet 605™ anti-human CD45RA 100 tests 304134 Brilliant Violet 510™ Mouse IgG2a, κ Isotype Ctrl 100 tests 400268 Brilliant Violet 605™ anti-human IFN-γ 100 tests 502536 Brilliant Violet 711™ Mouse IgG1, κ Isotype Ctrl 100 tests 400168 Brilliant Violet 605™ anti-human IL-4 100 tests 500828 Brilliant Violet 785™ Mouse IgG1, κ Isotype Ctrl 100 tests 400170 Brilliant Violet 650™ anti-human CD45 100 tests 304044 Brilliant Violet 650™ anti-human CD196 (CCR6) 100 tests 353426 PE/Dazzle™ 594 Armenian Hamster IgG Isotype Ctrl 25 μg 100 μg 400951 400952 Brilliant Violet 650™ anti-human IL-2 100 tests 500334 PE/Dazzle™ 594 Rat IgG1, κ Isotype Ctrl 25 μg 100 μg 400445 400446 Brilliant Violet 711™ anti-human CD197 (CCR7) 100 tests 353228 PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl Brilliant Violet 785™ anti-human CD45RA 100 tests 304140 25 μg 100 μg 400659 400660 FITC anti-human CD19 100 μg 302256 FITC anti-human CD45RA 100 μg 304148 FITC anti-human CD62L 100 μg 304838 FITC anti-human TCR Vβ13.1 25 tests 100 tests 362403 362404 Pacific Blue™ anti-human CD56 (NCAM) 25 tests 100 tests 362519 362520 Pacific Blue™ anti-human CD195 (CCR5) 25 tests 100 tests 359123 359124 PE/Dazzle™ 594 anti-human CD38 25 tests 100 tests 303537 303538 PE/Dazzle™ 594 anti-human CD45RA 25 tests 100 tests 304145 304146 PE/Dazzle™ 594 anti-human CD184 (CXCR4) 25 tests 100 tests 306525 306526 PE/Dazzle™ 594 anti-mouse/human KLRG1 (MAFA) 25 μg 100 μg 138423 138424 PE/Dazzle™ 594 anti-human IL-2 25 tests 100 tests 500343 500344 PE/Dazzle™ 594 anti-human IL-4 25 tests 100 tests 500831 500832 PE/Dazzle™ 594 anti-human IL-17A 25 tests 100 tests 512335 512336 PE/Dazzle™ 594 anti-human TNF-α 25 tests 100 tests 502945 502946 Purified anti-human CD19 25 μg 100 μg 363001 363002 Purified anti-human CD36L1 (SCARB1, SR-BI) 100 μg 363201 Isotype Controls Secondary Reagents Purified anti-human CD228 (MFI2, MTF1) 100 μg 363101 Description Size Cat. No. Purified anti-mouse/human SEMA4A 100 μg 148402 Brilliant Violet 650™ Streptavidin 100 μg 405232 PE/Dazzle™ 594 Streptavidin 25 μg 100 μg 405247 405248 Alexa Fluor® and Texas Red® are registered trademarks and Pacific Blue™ is a trademark of Life Technologies Corporation. Brilliant Violet™ is a trademark of Sirigen Group Ltd. eFluor® is a registered trademark of eBioscience. MaxPar® is a registered trademark of DVS Sciences. BioLegend products are manufactured in an ISO 9001:2008 and ISO 13485:2003 certified facility following GMP compliant procedures, ensuring the highest quality and stability. 9727 Pacific Heights Blvd.|San Diego, CA 92121 1.877.246.5343 (877-BIOLEGEND) International: 1.858.768.5800 biolegend.com 02-0001-31