BIOS 221 GENETICS LABORATORY

BIOS 221
GENETICS LABORATORY
Course Coordinator :
Email:
Office Hours :
Dr Shalu Shiv Mendiratta
[email protected]
By Appointment
Teaching Assistant : Specific for each section. Please ask at first session
TA contact Information : Specific for each section. Please ask at first session
Office Hours : Most of the labs do not require the entire lab period. You are
encouraged to discuss any questions or concerns with your TA before you leave
the lab for the day. If you need to meet with your TA outside of lab, contact
him/her to make an appointment.
COURSE OBJECTIVES
Genetics Laboratory course focuses on both Classical Genetics and molecular
genetics.
Genetics Lab course will
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Acquaint the students with the mechanics of experimentation and the
methods and techniques of experimental genetics
Familiarize the students with the genetic principles in a first hand manner
as they see these principles operate in a controlled experimental setting
Introduce students to learn plant and animal chromosomes and their
behavior in somatic cells and in sex cells.
Introduce students to learn about eukaryotic genetics using yeast as a
model.
Introduce students to classical genetics as they perform experiments with
the model organism Drosophila.
Introduce students to basic molecular genetics laboratory skills like
bacterial transformation, viral transduction, bacterial conjugation,
restriction endonuclease digestion of DNA, gel electrophoresis and
amplification of DNA by Polymerase Chain Reaction.
Train the students to learn and perform experiments, collect data, analyze
the data learn to interpret the data and draw conclusion from it.
Provide students with opportunities to develop science writing skills.
It is required that you are concurrently enrolled in Bios 220 or have taken this
course before Bios 221. Consequently, it is presumed that you will have access
to a general genetics text that will serve to provide the background and theory
about most of the experiments in the lab course. In addition during this course
BIOS 221
GENETICS LABORATORY
you will be provided with handouts that will further enhance your understanding
of the concepts and methodologies of the experiments that you will be performing
in the laboratory.
Carefully study the syllabus and pay special attention to deadlines, exam dates
etc. Immediately notify your TA if you have a conflict. Also notify me by email.
Always mention the day and time of your section when you send
me an email so I can help you better.
GRADING
The points for this course are derived from quizzes, lab reports, projects and
exams.
Possible Number of Points = 500
Written Exams (2 exams, 100 points each)
Quizzes (Top 10 at 10 points each)
Lab Report
Lab Presentations
Final Practical Exam
Participation
=
=
=
=
=
=
200
100
80
20
100
Required
- Exams and Lab reports are graded by the TA. The TA will discuss the exam in
class on the day that is indicated in the syllabus. You MUST return the exam
back to the TA before you leave the class. If you have any concerns with grading,
you must address them the day that the exam/report is returned. If there is not
enough time, return your exam to the TA and ask them to bring it to the next
class. You can send me a mail and discuss the exam with me, but this MUST be
done within the next session after returning the exam.
- NO REQUESTS FOR REGRADING PREVIOUS
ENTERTAINED AT THE END OF SEMESTER
EXAMS
WILL
BE
LABS
Do not enter the lab until your instructor asks you even if the door is open.
Be prepared to show your University ID when asked by your instructor at all time
in class and during exam. Failure to do so could result in loss of partial or total
points.
BIOS 221
GENETICS LABORATORY
The Teaching assistant in charge of your section is your instructor for this course.
You are expected to have read the handouts BEFORE the relevant class. Your
TA will explain to you the theory behind the experiment, provide a background for
the experiment, explain the methods and procedure to be followed in performing
the experiment and guide you in the analysis of the data that you collect.
If you feel that your TA is not explaining the background for the labs or not
answering your questions properly, let me know in the first few weeks so I
can talk to the TA. If you ask for points later on in the semester because
you feel your TA did not explain the topics well, I will not be able to do
anything.
You are expected to and are responsible for reading the background material
posted on Blackboard so that you are prepared for the lab. If you have any
questions or concerns about understanding of the concepts or the experiment,
you should address them during the lab period or in the subsequent labs. In
addition, there are ‘review’ sessions scheduled before the exam when you should
bring up any questions. You can also always email me any questions that you
may have or email to make appointment to meet with me.
QUIZZES
Quizzes are distributed at the beginning of your lab section and are collected
after ten minutes. If you come late to the lab without a valid reason and miss the
quiz, there is no way for you to make up that quiz. Incase of an emergency, or if
you have a valid reason, check with me. Your TWO lowest quiz scores will be
dropped in the calculation of your final point total. There are no make-up labs
unless your absence is due to a valid reason. Inform your TA AND me
immediately about any possible conflict with lab. Do not assume that it is ok for
you to miss lab due to travel plans. If you miss a lab without a valid excuse, you
could be penalized by 10 points per lab.
LATE REPORT
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You are given plenty of time to complete your lab report. Lab Reports are
considered late if submitted any later than 15 minutes after the scheduled
beginning of your lab section in which they are due.
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You will be penalized five points for each day that your lab report is late
beginning with the day that the report is due.
BIOS 221
GENETICS LABORATORY
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No requests such as not finding the printer or printer not working
will be entertained. If you anticipate any problems, print your report a
day or two in advance.
-
DO NOT email your report to your TAs. You must submit a printed
copy. If you have completed the report but have an emergency that
prevents you from getting to the campus, follow the instructions on late
report. When you submit the assignment, include documents that show
the nature of your emergency.
-
If there is any emergency because of which you can not come in to class
to turn in the report, notify your TA and me in advance so we can instruct
you appropriately.
MISSED EXAMS
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The exam dates are provided in the syllabus. If there is a valid conflict,
inform me immediately so that alternative arrangements can be made.
Students who miss an Exam without notifying me will get a zero on the
exam.
-
Make sure that you leave enough time to reach the lab on the exam day
so you do not miss the exam because of missed train or broken car.
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If you miss the exam without a valid reason you will be given a zero.
Please do not assume that your reason would be considered valid, inform
the TA AND me as soon as you can so that necessary steps can be taken
in time. DO NOT wait till the last minute to send an email as you may
receive a zero for the exam in such a situation.
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This policy is NOT valid for final exam. All students are required to give
the Final Exam.
ACADEMIC INTEGRITY
Cheating will not be tolerated. During the tests we will assume that you are
cheating if your eyes wander from your test paper to another’s. You will not be
allowed to carry cell phones, calculators etc to the exam. Any act of academic
dishonesty will be punished severely. Cheating includes, but is not limited to
BIOS 221
GENETICS LABORATORY
falsification of data, plagiarism of published material, copying another student’s
work and accepting or giving help during quizzes or exams.
The UIC Guidelines regarding academic Integrity may be found at
http://www.uic.edu/ucat/catalog/GR.shtml
BOOKS
1. REQUIRED: Symbiosis Custom Lab Manual
BIOS 221-Genetics Laboratory
ISBN 0536897999
2. REQUIRED: Schaum’s Outlines Genetics Fourth or Fifth Edition by Susan
Elrod and William Stansfield.
ISBN 0071362061
3. (Optional) Flagg, R.O.; Carolina Drosophila Manual, Burlington, NC:
Carolina Biological Supply;
ISBN 0892780274
BIOS 221 Genetics laboratory
Lab Schedule
SOG (4) = SCHAUM’S OUTLINES GENETICS 4th edition
SOG (5) = SCHAUM’S OUTLINES GENETICS 5th edition
LM = SYMBIOSIS LAB MANUAL
***The syllabus is subject to changes due to availability of reagents***
Date
Activities
Aug 26/27
Aug 28/29
Introduction
Quiz 1 covers Human Chromosome
Human Karyotype
Page
Syllabus
- Handout
- LM 41 -51
- SOG(4) p. 217-254
- SOG (5) p.182-211
BIOS 221
GENETICS LABORATORY
Sep 2/3
Sep 4/5
Labor Day Holiday. No classes
Quiz 2 covers Mitosis and Meiosis
Mitosis and Meiosis
a. Making slides for different stages of mitosis from
onion root tip
b. Observing slide sets
1. Plant Mitosis
2. Animal Mitosis
3. Meiosis
Sep 9/10
Quiz 3 covers Probability
a. Probability and Probability practice questions
b. Pedigree Analysis Handout
Sep 11/12
- Handout
- SOG(4) p. 6-27
- SOG(5) p. 2-22
Quiz 4 covers corn genetics
a. Corn Genetics
Ignore ‘duplicate genes with cumulative effect’
b. Chi - Square Analysis
- Handout
- SOG(4) p.43-49
SOG(5) p.31-34, solved
problems 2.8-2.12 on
p.46-47
- LM p. 19-26
- Handout
- SOG(4) p. 64-65, and
73
SOG(5) p.42, solved
problem 2.22 p.52, 2.822.83 p.59
- LM p. 119-121
- Handout
- SOG(4)p.28-38, p.4955 and p.117-124
-SOG(5)p.23-29, p.35-38
and p.97-99, solved
problems 2.1-2.4 p.4344, 2.13-2.15 p.47-49
and 4.1-4.2 and 4.4-4.6
p.101-105
- LM 8-10, 11, 14-15
- Handout
- LM 27-39
- SOG(4)p.58-64
- SOG(5)p.40-42, solved
problem 2.18-2.21 p.5052
Sep 16/17
Quiz 5 covers Micro-Measuring and Bacterial
Conjugation
a. Micro-Measuring
b. Bacterial Conjugation Part I
Sep 18/19
- Handout
BIOS 221
GENETICS LABORATORY
a. Bacterial Conjugation Part II
Sep 23/24
Sep 25/26
Sep 30 /
Oct 1
Oct 2/3
Oct 7/8
Bacterial Conjugation Part III
Quiz 6 covers Viral Transduction
- Handout
- Handout
a. Viral Transduction
- Handout- SOG(4)p.367-376
- SOG(5)p.302-308,
solved problem 11.1
p.320
b. Viral Transduction II
- Handout
Quiz 7 covers Bacterial Transformation
a. Bacterial Transformation, Part I
- Handout
-SOG(4)p.336-338
-SOG(5)p.275-276
b. Bacterial Transformation, Part II
- Handout
Review
Oct 9/10
Oct 14/15
EXAM I
Covers material from
week 1–week 7
Quiz 8 covers Drosophila Genetics
a. Stages of life cycle, identify male & female,
identification of mutant eyes
b.Sex determining Mechanism
c. Sex-linked inheritance
- Carolina Drosophila
Manual p. 4-18 ( upto
Experimental Crosses)
- SOG p. 140-144
- SOG p. 146-149
- LM p. 1-8, 11-14
- Handout
d.Set up Monohybrid and Dihybrid Drosophila
Crosses
Oct 16/17
Quiz 9 covers Yeast Complementation
- Handout
a. Complementation in Yeast-Part I
Exam 1 returned
Oct 21/22
- Handout
- LM p. 69-74
a. Complementation in Yeast-Part II
b. Sordaria Genetics
Oct 23/24
Oct 28/29
a. Complementation in Yeast-Part III
Quiz 10 covers Restriction Endonuclease
Digestion of DNA
a. Restriction Endonuclease Digestion of DNA, Part I
b. Complementation in Yeast-Part IV
c Drosophila Crosses (continued)
- Handout
- Handout
- LM p75-78
- SOG(4) p. 410-419
-SOG(5) p.334-339,
solved problem 12.2
p.359
BIOS 221
GENETICS LABORATORY
Oct 30/31
Nov 4/5
a. Restriction Endonuclease Digestion of DNA, Part II
Room 3085
Quiz 11 covers PCR Amplification of DNA
a. PCR-I
- Handout
- LM p85-87
- Handout
- SOG(4) p. 419- 421
- SOG(5)p.339-341
Room 3099
Presentations
Nov 6/7
Room 3085
a. PCR- II- Analysis of PCR product
- Handout
Room 3099
Presentations
Nov 11/12
Room 3085
Presentations
Room 3099
Quiz 11 covers PCR Amplification of DNA
- PCR-I
Nov 13/14
Room 3085
Presentations
- Handout
- SOG(4) p. 419- 421
- SOG(5)p.339-341
- Handout
Room 3099
a. PCR- II Analysis of PCR product
Nov 18/19
Quiz 12 covers Blood Groups
a. Blood Groups
- Handout
b. Polygenic Inheritance
Finger print ridge count
- Handout
- LM p. 97-105
- LM p. 112-114
Drosophila Report Due for 3085 and 3099
Nov 20/21
Population Genetics
The Hardy-Weinberg Principle -PTC tasting
Review
- Handout
-SOG(4) p. 299-306
- SOG(5) p.248-251,
solved problems 9.1-9.6
p.255-257
- LM p. 107-112 (upto
“Part III”)
Nov 25/26
EXAM 2
BIOS 221
GENETICS LABORATORY
Nov 27/28
Dec 2/3
Thanksgiving holiday. No classes.
EXAM 2 returned
Drosophila report returned
Final Review Session
Dec 4/5
FINAL PRACTICAL EXAM
Covers all material