Addendum to Alba Meeting in pdf format

Oxygen Club
of California
University
of Turin
OXIDANTS AND ANTIOXIDANTS IN BIOLOGY
BOOK OF ABSTRACTS: ADDENDUM
7-10 SEPTEMBER 2005
ALBA, ITALY
OXIDANTS AND ANTIOXIDANTS IN BIOLOGY
A MEETING IN HONOR OF
ANGELO AZZI
7-10 SEPTEMBER 2005
ALBA, ITALY
____________
A JOINT MEETING OF
OXYGEN CLUB OF CALIFORNIA
UNIVERSITY OF TURIN
_____________
ORGANIZERS
Giuseppe Poli
DEPARTMENT OF CLINICAL AND BIOLOGICAL SCIENCES, UNIVERSITY OF TURIN,
SAN LUIGI HOSPITAL, ORBASSANO, TURIN, ITALY
Helmut Sies
INSTITUT FÜR BIOCHEMIE UND MOLEKULARBIOLOGIE I,
HEINRICH-HEINE-UNIVERSITÄT DÜSSELDORF, DÜSSELDORF, GERMANY
Enrique Cadenas
DEPARTMENT OF MOLECULAR PHARMACOLOGY & TOXICOLOGY, SCHOOL OF PHARMACY,
UNIVERSITY OF SOUTHERN CALIFORNIA, LOS ANGELES, CA, USA
CONTENTS
ADDITIONAL POSTER ABSTRACTS ........235
LIST OF PARTICIPANTS................240
LIST OF SPONSORS....................264
Resveratrol protects against 4-Hydroxynonenalinduced apoptosis: a role for JNK, AP-1 and caspases.
Ozgur Kutuk1, Giuseppe Poli2, Huveyda Basaga1
1
Biological Sciences and Bioengineering Program, Sabanci Univ,
34956 Tuzla, Istanbul,Turkey, e-mail: [email protected]
2
Department of Clinical and Biological Sciences University of Turin at
St. Luigi Gonzaga Hospital,10043-Orbassano, Turin, Italy.
In the present study we have studied the effect of resveratrol
in signal transduction mechanisms leading to apoptosis in
3T3 fibroblasts when exposed to one of the major end
product of oxidized fatty acid metabolism, 4hydroxynonenal (HNE). Mitogen-activated protein kinases
(MAP kinases) and various caspases have been proposed to
mediate stress-induced apoptosis in many cell types. HNE
induced early activation of JNK and p38 proteins but
downregulated the basal activity of ERK 1/2. We were also
able to demonstrate the release of cytochrome c from
mitochondria, caspase-9 and caspase-3 activation as shown
by active caspase fragments and fluorometric caspase
assays. Treatment of fibroblasts with specific inhibitors of
MAPKs and caspases provided further mechanistic
evidence for JNK, caspase-9 and caspase-3 involvement but
neither p38 nor ERK 1/2 in mediating HNE-induced
apoptosis. Studies undertaken by MAPK and caspase
inhibitors as well as resveratrol indicated that resveratrol
was as effective as JNK inhibitor in preventing JNK and
caspase activation hence apoptosis. Increase in c-Jun and
phospho-c-Jun and decrease in c-Fos protein levels took
place within 1 h of HNE treatment which was accompanied
by an increase in the DNA binding of AP-1. This effect
could be overcome by pretreatment of cells with resveratrol
as shown by immunoblots and gel shift assays.
Overexpression of dominant negative c-Jun and JNK1
235
prevented HNE-induced apoptosis, which indicates a role
for JNK-c-Jun/AP-1 pathway in HNE-induced apoptosis.
In light of the JNK-dependent induction of c-Jun
transcription and AP-1 upregulation induced by HNE and
the protective role of resveratrol, these data may show a
critical potential role for JNK in the cellular response
against toxic products of lipid peroxidation. In this respect
resveratrol acting through MAP kinase pathways and
specifically on JNK could have a role other than acting as
an antioxidant.
236
Oncolyn inhibits telomerase activity, induce apoptosis of
cancer cells, cause regression and clinical remission of
solid tumors
Arthur H.K. DJang, M.D., Ph D.1
Lai Zho-Sheng, M.S.2, Zhou Dian-Yuan, M.D. 2, M.Bud Nelson, Ph.D.,
J.D.1, Mikhael Adams, N.D.3, Jiang Bai, M.D., Ph.D. 2, Zhang Zhenshu,
M.D., Ph.D. 2, Zhang Yali, M.D., Ph.D. 2, Zhang Yanshu, M.B.,4 ,Dimitrios
Galaris, Ph.D.5
Santé International, Inc., New York, USA, 1, Institute of Digestive Medicine, First
Military Medical University, China, 2, Renascent Integral Health Centre, Toronto,
Canada, 3, North China Mining Medical College, China,4 , University of Ioannina
Greece, 5
In general, telomerase expression is a hallmark of cancer.
Telomerase activity indicates the capacity for malignant cells’
unlimited proliferation and immortality. Telomerase inhibitors
are attractive agents against this phenomenon.There are intense
worldwide efforts, 1, 2, for this endeavor; i.e, to discover agents
that selectively destroy cancer cells without harming normal
tissue. Oncolyn® has been demonstrated previously to possess
anti-oxidative, anti-inflammatory, anti-angiogensis, antimetastasis, immune augmentation, dismutagenic and
cytoprotective activities, as well as, promote cancer cell
apoptosis.
Oncolyn was demonstrated to protect DNA against H2O2, silica
and asbestos particles by single cell gel electrophoresis and
histopathology.
Effective
management
of
malignant
mesothelioma verified by CT will be included.
The present data further demonstrates the positive correlation of
telomerase inhibition by Oncolyn with cancer cell apoptosis, in
providing a molecular basis for Oncolyn’s anti-cancer function,
prevention and therapy.
Oncolyn is composed of edible plant extracts with main
ingredients including flavonoids, polyphenols, phenolic acids
and saponins. It protects skin from UV damage, respiratory
237
system from silica and asbestos, endothelium from ox LDL. It
has shown anti-cancer function in nude mouse against various
cancer cells implants both for prevention and therapy.
Inhibition of telomerase results in erosion of telomeres, and
eventful cessation of cell proliferation and apoptosis.
Tables of telomerase inhibition with correlation of cancer cells’
apoptosis and summary of clinical application of Oncolyn
against various malignancies will be included.
Literature cited
1 Enzyme stopper combats cancer, Science News, May 28, 2005, 167-349
2 Efficacy of telomerase inhibitor drug discussion, American Association for Cancer Research
2005, Annual Meeting, May, Anaheim, California.
238
Ascorbate and nitrite react under physiologically
relevant conditions to give a novel product and not NO
Milos Filipovic1, Dragana Stanic1 and Vesna Niketic2
1
ICTM-Center for Chemistry, University of Belgrade
Department of Chemistry, University of Belgrade
2
Nitric oxide, an important regulator of a variety of
biological functions, is synthesized by complex enzymes
known as nitric oxide synthases. Recently, a novel pathway
for NO production in humans that involves either direct
disproportion or reduction of nitrite to NO under the acidic
and/or highly reductional conditions was discovered. The
latter includes ascorbic acid, which reduces nitrous acid
rapidly into NO under acidic conditions. In the present work
we provide direct evidence that ascorbate and nitrite react at
physiological pH in 2:1 stoichiometry to yield a previously
unidentified product: 3, 3’-[(hydroxyimino)bis(oxy)]bis(ascorbic acid). The product was purified and its structure
was confirmed by 13C, 1H-NMR, IR spectra and MS-ESI.
Product spontaneously decomposes releasing NO, which
was detected electrochemically, and its bioactivity
confirmed with organ chamber method. Kinetics of NO
release was determined by oxyhemoglobin assay. Transition
metal ions (iron, copper) catalyze the release of nitric oxide.
These findings are highly relevant for explaining the
phenomena related to the nonenzymatic production of NO
in human macro-phages and the nitrite-rich Dietary
Approaches to Stop Hypertension-diet induced hypotensive
effects. Furthermore, the product of ascorbate and nitrite
may serve as a potential pharmacological donor of NO,
which could decrease the vascular tone and increase the
tissue blood flow.
239
LIST OF PARTICIPANTS
Abbott Mariana Pedrinha
[email protected]
Brazil
Ahl Ing-Marie
[email protected]
Sweden
Albano Emanuele
[email protected]
Italy
Aldini Giancarlo
[email protected]
Italy
Ames Bruce
[email protected]
USA
Antunes Fernando Jose Nunes
[email protected]
Portugal
Aoki Yutaka
[email protected]
France
Aquilano Katia
[email protected]
Italy
Aragno Manuela
[email protected]
Italy
Arduini Alessandro
[email protected]
Spain
Arkan Melek Canan
[email protected]
Germany
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Arnér Elias
[email protected]
Sweden
Ashur Idan
[email protected]
Israel
Augustin Wolfgang
Germany
[email protected]
Azzi Angelo
[email protected]
USA
Baldovino Norman
norm82@libero-it
Italy
Banning Antje
[email protected]
Germany
Basaga Huveyda
[email protected]
Turkey
Battino Maurizio
[email protected]
Italy
Beretta Giangiacomo
[email protected]
Italy
Bermejo Bescós Palma
[email protected]
Spain
Bertoli Enrico
[email protected]
Italy
Biasi Fiorella
[email protected]
Italy
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Boccini Francesca
[email protected]
Switzerland
Boguszewska Anna
[email protected]
Poland
Borges Fernanda
[email protected]
Portugal
Boveris Alberto
[email protected]
Argentina
Brigelius-Flohé Regina
[email protected]
Germany
Brito Paula
[email protected]
Portugal
Cadenas Enrique
[email protected]
USA
Calabrese Vittorio
[email protected]
Italy
Calheiros Cruz Rita
[email protected]
Portugal
Canal Tiziana
[email protected]
Italy
Canali Raffaella
[email protected]
Italy
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Carini Marina
[email protected]
Italy
Castello Laura
[email protected]
Italy
Cavalca Viviana
[email protected]
Italy
Cavalieri Barbara
[email protected]
Italy
Ceriello Antonio
[email protected]
Italy
Chamulitrat Walee
Germany
[email protected]
Chessex Philippe
[email protected]
Canada
Chiarpotto Elena
[email protected]
Italy
Christen Stephan
[email protected]
Switzerland
Cillard Josiane
[email protected]
France
Cillard Pierre
[email protected]
France
Cimino Francesco
[email protected]
Italy
243
Ciriolo Maria R.
[email protected]
Italy
Comporti Mario
[email protected]
Italy
Cook Sarah L.
[email protected]
UK
Corona Giulia
[email protected]
Italy
Corti Alessandro
[email protected]
Italy
Corvi Mora Paolo
[email protected]
Italy
Coutinho Olga Pereira
[email protected]
Portugal
Cross Carrol E.
[email protected]
USA
Cutrin Juan Carlos
[email protected]
Italy
da Costa Ferreira Ana Maria
[email protected]
Brazil
Dalle Donne Isabella
[email protected]
Italy
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Davison Gareth
[email protected]
Ireland
Day Regina
[email protected]
USA
Degan Paolo
[email protected]
Italy
Del Val Greg
[email protected]
[email protected]
Switzerland
De Martino Angelo
[email protected]
Italy
Dessì Maria Assunta
[email protected]
Italy
Dever Gary
[email protected]
UK
Dianzani Mario Umberto
[email protected]
Italy
Dickinson Dale A.
[email protected],
[email protected]
USA
Djang Arthur H.K.
[email protected]
USA
Di Leva Agnese
[email protected]
Italy
245
Domenech de Antonio Elena
[email protected]
Spain
Domenicotti Cinzia
[email protected]
Italy
Dunker Manfred S.
[email protected]
Germany
Eichhorn Judith
[email protected]
UK
Erridge Clett
[email protected]
UK
Escobar Javier J.
[email protected]
Spain
Esposito Franca
[email protected]
Italy
Favier Alain
[email protected]
France
Fehlmann Max
[email protected]
Canada
Feijoo Montserrat
[email protected]
Spain
Felici Emanuela
[email protected]
Spain
Filipovic Milos R.
[email protected]
Serbia and Montenegro
246
Filomeni Giuseppe
[email protected]
Italy
Finckh Barbara
[email protected]
Germany
Flohé Leopold
[email protected]
Germany
Frade Joao
[email protected]
Portugal
Fraga César
[email protected]
USA
Franzini Maria
[email protected]
Italy
Froio Teresa
[email protected]
Italy
Galaris Dimitrios
[email protected]
Greece
Galletti Sara
[email protected]
Italy
Gamba Paola
[email protected]
Italy
Gambini Juan
[email protected]
Spain
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Gargiulo Simona
[email protected]
Italy
Gellerman Werner
[email protected]
USA
Giusti Anna Maria
[email protected]
Italy
Gómez Gómez Carmen
[email protected]
Spain
González Rául
[email protected]
Spain
Gruber Florian
[email protected]
Austria
Guarnieri Serena
[email protected]
Italy
Gwozdzinski Krzysztof
[email protected]
Poland
Han Derick
[email protected]
USA
Handa Osamu
[email protected]
[email protected]
Japan
Hari Siva P.
[email protected]
USA
248
Hatao Masato
[email protected]
Japan
Heller Regina
[email protected]
Germany
Hernandez Eva
[email protected]
UK
Hiller Karl-O.
[email protected]
Germany
Hofstetter Dustin
[email protected]
Switzerland
Holmgren Arne
[email protected]
Sweden
Ichijo Hidenori
[email protected]
Japan
Iles Karen E.
[email protected]
USA
Izzotti Alberto
[email protected]
Italy
Jansson Patric J.
[email protected]
Finland
Jessup Wendi
[email protected]
Australia
Jones Dean
[email protected]
USA
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Junqueira Virginia
[email protected]
Brazil
Kadl Alexandra
[email protected]
USA
Kartal-Özer Nesrin
[email protected]
Turkey
Kambe Tetsuya
[email protected]
Japan
Kawanishi Shouske
[email protected]
[email protected]
Japan
Klotz Lars Oliver
[email protected]
Germany
Kokura Satoshi
[email protected]
Japan
Kondo Norihiko
[email protected]
Japan
Krutmann Jean
[email protected]
Germany
Laggner Hilde Gard
[email protected]
Austria
Laranjinha Joao
[email protected]
Portugal
250
Ledo Ana Margarida
[email protected]
Portugal
Leighton Federico
[email protected]
Chile
Leitinger Norbert
[email protected]
USA
Leonarduzzi Gabriella
[email protected]
Italy
Leoncini Emanuela
[email protected]
Italy
Lillig Christopher Horst
[email protected]
Sweden
Lisovskaya Irina L.
[email protected]
Russia
Livrea Maria A.
[email protected]
Italy
Lloret Ana
[email protected]
Spain
Lupino Stefania
[email protected]
Italy
Maffei Facino Roberto
[email protected]
Italy
Maggiora Marina
[email protected]
Italy
251
Maguire John
[email protected]
[email protected]
USA
Maina Marco
[email protected]
Italy
Maiorino Matilde
[email protected]
Italy
Makarov Valery G.
[email protected]
Russia
Makarova Marina N.
[email protected]
Russia
Mann Giovanni
[email protected]
UK
Mantello Pierre
[email protected]
France
Maraldi Tullia
[email protected]
Italy
Marcocci Lucia
[email protected]
Italy
Marinari Umberto M.
[email protected]
Italy
Markovic Jelena
[email protected]
Spain
252
Martina Valentino
[email protected]
Italy
Martins Maria João
[email protected]
Portugal
Marzani Barbara
[email protected]
Italy
Mascia Cinzia
[email protected]
Italy
Massarenti Paola
[email protected]
Italy
Mastrocola Raffaella
[email protected]
Italy
Masutani Hiroshi
[email protected]
Japan
Matsui Hirofumi
[email protected]
Japan
Meydani Mohsen
[email protected]
USA
Meydani Simin
[email protected]
USA
Milzani Aldo
[email protected]
Italy
Miranda-Vizuete Antonio
[email protected]
Spain
253
Mirault Marc-Edouard
[email protected]
Canada
Mitjavila Maria Tersa
[email protected]
Spain
Mora Nancy J
[email protected]
Spain
Moulas Anargyros N
[email protected]
Greece
Muntané Jordi
[email protected]
Spain
Muzio Giuliana
[email protected]
Italy
Nakamura Masao
[email protected]
Japan
Natella Fausta
[email protected]
Italy
Navarro Ana
[email protected]
Spain
Negre-Salvayre Anne
[email protected]
France
Nesaretnam Kala
[email protected]
Malaysia
254
Neufang Gitta
[email protected]
Germany
Nordstrom Tommy
[email protected]
Finland
Nunes Carla
[email protected]
Portugal
Ogru Esra
[email protected]
Australia
Oteiza Patricia
[email protected]
USA
Paker Lester
[email protected]
USA
Park Ah-Mee
[email protected]
USA
Pasternak Kazimierz
Poland
Patel Mulchand
[email protected]
USA
Pena Deudero Juan Josè
[email protected]
Spain
Perrelli Maria Giulia
[email protected]
Italy
Picklo Matthew J.
[email protected]
USA
255
Pina Enrique
[email protected]
Mexico
Poli Giuseppe
[email protected]
Italy
Polidori Cristina
[email protected]
Germany
Pompella Alfonso
[email protected]
Italy
Popova Natalia A.
[email protected]
Russia
Portes Pascal
[email protected]
France
Pozzi Roberto
[email protected]
Italy
Prata Cecilia
[email protected]
Italy
Pritsos Chris A.
[email protected]
USA
Pritsos Karen L.
[email protected]
USA
Pronzato Maria Adelaide
[email protected]
Italy
Radi Rafael
[email protected]
Uruguay
256
Rallis Michail
[email protected]
Greece
Ramadori Pierluigi
[email protected]
Italy
Reimann Juergen
[email protected]
Germany
Reiter Elker
[email protected]
Switzerland
Rimbach Gerald
[email protected]
Germany
Riso Patrizia
[email protected]
Italy
Robbesyn Fanny
[email protected]
Italy
Rogovin Jarrow L.
[email protected]
USA
Romano Antonino
[email protected]
Italy
Romero Gomez Francisco J.
[email protected]
Spain
Rossi Luisa
[email protected]
Italy
257
Royo Dolores
[email protected]
Spain
Sakhi Amrit Kaur
[email protected]
Norway
Scalbert Augustin
[email protected]
France
Schaur Rudolf J.
[email protected]
Austria
Schwarzer Evelin
[email protected]
Italy
Sena Cristina
[email protected]
Portugal
Serviddio Gaetano
[email protected]
Italy
Shamsaei Elham
[email protected]
UK
Shcherbachenko Irina M
[email protected]
Russia
Sies Helmut
[email protected]
Germany
Silva Joao Pedro
[email protected]
Portugal
Simon-Giavarotti Karin
[email protected]
Brazil
258
Sitia Roberto
[email protected]
Italy
Smith Mark
[email protected]
USA
Soodaeva Svetlana K.
[email protected]
Russia
Sottero Barbara
[email protected]
Italy
Sowden Rebecca
[email protected]
UK
Spickett Corinne
[email protected]
UK
Squillerio Isabella
[email protected]
Italy
Stahl Wilhelm
[email protected]
Germany
Stanic Dragana
[email protected]
Serbia and Montenegro
Steiling Heike
[email protected]
Switzerland
Sukiasyan Astghik
[email protected]
Armenia
259
Sury Matthias
[email protected]
Switzerland
Suzuki Hisanori
[email protected]
Italy
Suzuki Yuichiro
[email protected]
USA
Sztanke Malgorzata
Poland
Takagi Tomohisa
[email protected]
Japan
Tamborra Rosanna
[email protected]
Italy
Tarozzi Andrea
[email protected]
Italy
Tedesco Doriana
[email protected]
Italy
Terao Junji
[email protected]
Japan
Tessitore Luciana
[email protected]
Italy
Tikhonov Vladimir P.
[email protected]
Russia
Trappoliere Marco
[email protected]
Italy
260
Traverso Nicola
[email protected]
Italy
Tsuji Tomoko
[email protected]
Japan
Uchida Koji
[email protected]
Japan
Unterhorst Goetz
[email protected]
Italy
Ursini Fulvio
[email protected]
Italy
Valacchi Giuseppe
[email protected]
USA
Valdez Laura Beatriz
[email protected]
Argentina
Valles Soraya L.
[email protected]
Spain
Veglia Fabrizio
[email protected]
Italy
Vernole Patrizia
[email protected]
Italy
Vidali Matteo
[email protected]
Italy
Vigliocco Giorgia
[email protected]
Italy
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Vina José
[email protected]
Spain
Vinokur Vladimir
[email protected]
Israel
Violi Francesco
[email protected]
Italy
Vita Joseph
[email protected]
USA
Vizio Barbara
[email protected]
Italy
Voß Peter
[email protected]
Germany
Williamson Gary
[email protected]
Switzerland
Wiswedel Ingrid
Germany
[email protected]
Wright James S.
[email protected]
[email protected]
Canada
Yodoi Junji
[email protected]
Japan
Zambonin Laura
[email protected]
[email protected]
Italy
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Zaobornyj Tamara
[email protected]
[email protected]
Argentina
Zarkovic Neven
[email protected]
Croatia
Zarkovic Kamelia
[email protected]
Croatia
Zentella Martha
[email protected]
Mexico
Zingaro Lorenzo
[email protected]
Italy
Zingg Jaun Mike
[email protected]
Switzerland
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SPONSORS
Fondazione Ferrero
Cavalieri Ottolenghi Foundation
BASF
Abkit, Inc.
Actimex
Alexis Biochemicals
Beiersdorf AG
Cassella-med GmbH
Cognis, Germany
Danone Vitapole
Dompè Pharmaceutical
DSEA, Dietary supplement Education Alliance
DSM Nutritional Products, Inc.
Fratelli Ceretto
Jarrow Formulas
Jarrow Industries
Johnson & Johnson
Leiner Health Products
LI Consulting
Nestlé
Novartis
Orthomol GmbH
Osato Research Institute
Pharmanex Research Institute
Phosphagenics
School of Pharmacy, University of Southern California
The Colgate Palmolive Company
Town of Alba
Turin Committee for 2006 Winter Olympics
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