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Microbial Methane Oxidation at Cover Soil Layer of Municipal Solid Waste
Landfill in Japan
Tomonori Ishigakt", Masato Yamada', Kaoru Ikeda2, Takuji Higashikawa2
1:National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
2: Ryukoku University, 1-5, Seta-Oe, Otsu, Shiga, Shiga 520-2194, Japan
*corresponding author: [email protected]
ABSTRACT
Greenhouse gas emission from waste landfill had been gained public concern. Waste landfill is one of the
large emission sources and
.
Reminder to authors:
Authors should prepare this Extended Abstract within 4 page by following this format. It is allowed to
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.......... These results revealed that the methane-oxidizing bacteria in the landfill cover will consume the
oxygen that penetrated from surface and net efficiency of methane reduction must be reduced. (less than 300
words)
Keywords:
methane oxidizing bacteria, greenhouse gas, mitigation, landfill cover soil
(3-5 keywords should be appeared)
INTRODUCTION
It
2000)
has
been well
·······················
recognized
that
a
waste
landfill
is major
emission
source
(IPCC,
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....
.
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...
activity has been investigated. The relationship between these parameters was analyzed for
understanding the mechanisms.
MATERIALS
AND METHODS
Subjected landfill site
A waste landfill site where municipal solid waste had been disposed of for 25 years was selected for
investigation. The details of the landfill was shown in Table I.
.
Table 1. Details oflandfill subjected in this study
Wastetype
duration
Capacity
Others
Landfill AM
MSW
1980-1995
750,000m3
Mountain
Landfill AN
MSW
1988-2000
900,000m3
plain
---------Microbial DNA extraction
Fifty grams of soil sample was suspended in 450 ml of distilled water for 30 minutes. Supernatant was
applied to gene extractionkit
.
RESULTS AND DISCUSSION
Distribution
of methane emission and oxidation in the landfill surface
Figure 1 shows the relationship between the methane emission from the surface of landfills and the
methane oxidation
.
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.
,'it
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25
•landfill AM
20
,-...,
~
'-'
i::
.....0
~ 10
"d
.....
><:
0
Q)
§
~.•....
8
• landfill AN
•
15
.t. landfill SS
•
X landfill FS
5
0
0
0.2
0.4
0.6
0.8
1
methane emission (rnl/m2/min)
Figure 1. Relationship of methane emission and oxidation
Population of methane-oxidizing bacteria in the landfill cover soil
Population of methane-oxidizing bacteria in the landfill surface is shown in Table 2. Relationship between
the population and methane emission was also shown in
.
CONCLUSION
This study aimed to propose a practical methodology for estimating the methane oxidation behavior of
waste landfills. Precise prediction of methane emission must be achieved by investigating the historical
trends of disposal of the organic
.
In this sense, we should continue to establish area-, region-, or at least country-specific activity and
kinetic parameters on methane oxidation in landfills beyond the case study.
ACKNOWLEDGEMENT
This research was supported by Global Environment Research Fund (B-071) by Ministry of the
Environment,Japan
REFERENCES
Intergovernmental Panel on Climate Change, Good practice guidance and uncertainty management m
Nationalgreenhouse gas inventories, 2000.
Ishigaki,T., Yamada, M., Nagamori, M., Ono, Y., and Inoue, Y.,Estimation of methane emissionfrom whole
waste landfill site using correlation between flux and ground temperature, Environmental Geology, 48, 7,
845-853,2005.
United Nations Framework Convention on Climate Change, Kyoto Protocol to the United Nations
FrameworkConvention on Climate Change, FCCC/CP/1997/L.7/Add.l, 1997.
References should be included in ·the text using Authors' surnames followed by the year of publication
(Pivatoand Ferguson, 2008). If there are more than two authors, the surname of the first two authors could
be mentioned,followed by "et al" (ex: Cossu R., Raga R. et al., 2008). Please use Normal style (not Italic).