www.twistdx.co.uk TwistFlow® Red Snapper Quick Guide

TwistFlow® Red Snapper Quick Guide
Part Number: TFRSNAP01Guide | Revision A
Lateral Flow Protocol
- Twirla™
Components
9 x Red Snapper reactions
9 x Lateral flow cassettes
9 x 250μl Buffer
9 x Disposable swabs
9 x 100μl Disposable pipettes
1 x Twirla™
Swab Protocol
1) Remove swab from sleeve.
2) Swab fish flesh thoroughly taking
care not to touch any other fish to
be tested with the swab.
3) Add swab to a 250μl Buffer tube and
mix well to create eluate for assay.
4) Dispose of swab.
Assay Protocol
1) Ensure batteries are in the Twirla™
device. The device is pre-programmed
for a 10 minute run at 40°C.
2) Start Twirla™ device by pressing PRE-HEAT
button.
3) Ensure Twirla™ device is up to temperature
- “At Temperature” will be lit (approx 3 mins).
4) Label reaction tubes and open lids of tubes.
5) Add 100μl of the eluate to the correctly
labelled tube using the disposable pipette.
Add 100μl of buffer to a seperate tube as a
negative control using a new pipette (optional).
6) Mix well by shaking, pooling the solution
at the bottom of the tube with the last flick.
7) Place in device and press ‘GO’.
8) At 10 minutes the device will beep.
Remove tubes from device.
9) Remove detection cassette and holder
from foil package during above incubation.
10) Place the sample tube in the holder
(tube base pointing out) and fold close.
11) Insert the holder into the detection cassette
and push the handle closed SLOWLY.
12) The lower line is the test line for Red Snapper
and the upper line is the flow control line.
WARNING: IF TUBES ARE OPENED
AFTER AMPLIFICATION THERE IS A
GREAT RISK OF CONTAMINATION OF
WORK SURFACES WITH AMPLICON!
ENSURE
THAT
APPROPRIATE
AVOIDANCE MEASURES ARE TAKEN!
www.twistdx.co.uk
TwistFlow® Red Snapper Quick Guide
Part Number: TFRSNAP02Guide | Revision A
Lateral Flow Protocol
- Without Twirla™ kit
Components
9 x Red Snapper reactions
9 x Lateral flow cassettes
9 x 250μl Buffer
9 x Disposable swabs
9 x 100μl Disposable pipettes
Swab Protocol
1) Remove swab from sleeve.
2) Swab fish flesh thoroughly taking
care not to touch any other fish to
be tested with the swab.
3) Add swab to a 250μl Buffer tube and
mix well to create eluate for assay.
4) Dispose of swab.
Assay Protocol
1) Preheat a heating block/water bath/
incubator to 40°C.
2) Label reaction tubes and open lids of tubes.
3) Add 100μl of the eluate to the correctly
labelled tube using the disposable pipette.
Add 100μl of buffer to a seperate tube as a
negative control using a new pipette (optional).
4) Mix well by shaking, pooling the solution at
the bottom of the tube with the last shake.
5) Place tube at 40°C for 4 minutes. At
4 minutes mix the tubes by shaking and
flicking the tube and return to 40°C for a
further 6 minutes.
6) Remove detection cassette and holder from
foil package during the above incubation.
7) Remove sample tube from 40°C and
place in the holder (tube base pointing
out) and fold close.
8) Insert the holder into the detection cassette
and push the handle closed SLOWLY.
9) The lower line is the test line for Red
Snapper and the upper line is the flow
control line.
WARNING: IF TUBES ARE OPENED
AFTER AMPLIFICATION THERE IS A
GREAT RISK OF CONTAMINATION OF
WORK SURFACES WITH AMPLICON!
ENSURE
THAT
APPROPRIATE
AVOIDANCE MEASURES ARE TAKEN!
www.twistdx.co.uk
TwistFlow® Red Snapper Quick Guide
TwistAmp® nfo Probe
Before amplification
Captured TwistAmp® nfo probe
Successful amplification
Capture of two co-joined
antigenic labels in an amplicon
To find out how to design your own TwistAmp® assays, please visit www.twistdx.co.uk
www.twistdx.co.uk