Sample Collection Instructions

258/A, 3rd Floor, Narayana Nethralaya Building, Narayana Health City,
Hosur Road, Bommasandra, Bangalore, Karnataka, India – 560 099
Sample Collection Instructions
Use the 20 ml syringe and 21G needle (provided) to draw maternal peripheral
blood by standard techniques.
Remove needle from the syringe (in sterile environment) and transfer the
contents into the Streck tubes immediately.
10 mL of blood in each of 2
Remove the stopper of the Streck tube by either gently rocking the stopper
from side to side or by grasping with a simultaneous twisting and pulling
action. A “thumb roll” procedure for stopper removal is NOT recommended
as tube breakage and injury may result.
cell-free DNA Streck Tubes
20-21 guage straight needles
No butterflies
Fill both tubes completely.
Vein collapse may require second venipuncture
Complete
label
10 mL of blood
Streck Tubes
+
Biohazard
Bag
+
pe
Cheek
Swab
(if obtained)
Gently invert each tube at least 10 times immediately after draw.
Gel Pack
Filled Envelope
Including the father's cheek swab is optional but recommended
when easily available.
A father's cheek swab may increase the chance of result on the first
blood draw, especially when fetal fractions are generally lower, as in
mothers with elevated weight or early in pregnancy
Cardboard Mailer
+
Requisition
Form
There is a preservative in the tube that protects the cells during
transport. It is critical that this preservative is gently mixed with the
entire blood volume by completely inverting the tube ten times. If the
sample is not completely and throughly, mixed a fraction of the cells
may lyse and release additional maternal DNA into the plasma
requiring a second sample
Do not eat 30 minutes prior to collection.
Rinse mouth with clean water.
Cardboard
Mailer
Do not freeze or
refrigerate
10 mL of 37°
blood
C
Streck Tubes
Remove cheek brush from tube and rub against inner lining of father’s
cheek for 60 seconds.
Rinse Mouth
30 minutes
prior prior
30 minutes
to collection
to collection
Place the swab in the same tube and then re-attach cap securely to tube.
High or low temperatures may cause the cells to lyse and release
additional maternal DNA into the plasma, thus diluting the fetal
component and requiring a second sample
Complete information on tube labels.
Complete
label
+
Replace both tubes and the cheek swab in slot provided in the box.
Ensure that the Test requisition form and consent form is complete and
placed in the plastic bag provided.
Cheek
Swab
(if obtained)
6° C
NIPT Box
Complete the check list.
Do not freeze or
refrigerate
+
+
Cardboard Mailer
Leave the filled tubes in the box at room temperature till collection for
transport. (Temperature range 60C - 360C)
+
6° C
Requisition
Consent
Cheek
Metallic Envelope
Form Requisition Form
Form
Contact the local Medgenome representative to arrange for shipping to
the laboratory.
37° C
Swab
(if obtained)
Toll Free No: 1800 102 8401
NIPT Box
Do not store kits in a refrigerator or freezer.
Cardboard
Mailer
www.medgenome.com
[email protected]
As per “Pre-Natal Diagnostic techniques(Regulation and prevention of Misuse) Rules, 1996” the lab strictly does not determine the sex of the fetus
MG NIPT SAMPLE COLL. INST. 19-05-2015 V1
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