Link

ISBN: 978-602-9042-86-3
PROCEEDINGS
The 4'h lnternational Seminar of lndonesia Society
and IUMS-ISM Outreach Program on Food
I]IlllllITSIA]I ilIIGRllBIA1 BTSllUBGES:
IIIUTRSITY A]III GlllBAl IilIPAGI
Organised by:
lndonesian Microbiology society (IMIS)
and lnternational Union of [,{icrobiologica] (lUt/S)
Supported by
:
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PROCEEDINGS
PROCEEDINGS
The 4'h International Seminar of Indonesia Society for
Microbiology and IUMS-ISM Outreach Program
on Food Safety
..INDONESIAN MICROBIAL RESOURCES
DIVERSITY AND GLOBAL IMPACT"
22"d-24hJsne 2011
Udayana University Denpasar-Bali, Indonesia
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UDAYANA T'NIVERSITY PRE S S
2012
:
PROCEEDINGS
The 4s International Seminai oflndonesia Society for Microbiology
and IUMS-ISM Oufreach Program on Food Safety
"INDONESIAN MICROBIAL RESOURCES
DIVERSITY AI.ID GLOBAL IMPACT"
22d -246 J urc 201
l,
Udayana Uniyersity Denpasar-Bali, Indonesia
Editors:
Dr. Yan Ramona
Dr. Yenni Ciawi
Dr. dr. Dewa Made Sukrama, Sp.MK.
Dr. I Nengah SujaYa
Dr. I Nengah Kencana Putra, MS.
Prof. Ir I Nyoman SemadiAntara, MP., Ph.D.
Prof.
Ir
Nuki B Nugroho, MSi.
I Wayan Redi Aryant4 M.AppSc., Ph.D.
Prof. Dr. dr. I Ketut Sukardik4 Sp.MK.
Dr. I Putu Suparthana, SP, M.Agr.
ir Ida Bagus Wayan Gunam,
'
:
MP., Ph.D.
Dr. Ir. Koesnandar , MEng.
Diana Nurani, MSi.
Prof. Dr. Endang S Rahayu
Published by:
Udayana University Press
Kampus Universitas Udayana Denpasar
2012,xxiii + 319 pages,2l x29,7 cm
ISBN: 978-602-9042-86-3
fhe
4h
Interhational Seminor ollndokesia SocieyJor Microbiolog'and IIJMSJSM Outreach progran on Fod Salety
''lndonesian Microbial Resourc?s: Dtversity and Abbal Inwct
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PREFACE FROM THE CHAIRMAN
OF THE ORGANIZING COMMITTEE
On behalfofthe organizing committee, I would like to extend my warmest greeting to all ofyou. I
would also like extend my appreciation to all ofyou, especially to those who have traveled far away to Bali
from their origin to attend this conference. This conference has been designed to gather scientists, engineers,
practitioners, and industries in microbiological related disciplines, so that they can discuss and share their
expertise in the fields oftricrobiology related issues. Fronr this intense discussion, it is expected that some
brilliant ideas to be used to improve the quality of hurr.ran life can be formulated, so that it is in line with
theaheme ofthe corrference: "lndonesian Microbial Resources: Diversity and Gobal Impacf',
h this oppotlunity I u'ould like to report briefly about this conference to President ofthe Indonesian
Society for Microbiology. The conlerence will consist of 12 plenary presentations delivered by Intemational
invited speakers from Italy, Netherlands, Japan, Thailand, Singapore, and Indonesia, covering general
aspects of Microbiology. Besides this plenary session, we will also have three satellite symposia, covering
areas of Health and Pharmacy, food and agriculture, and Industry and enyironment. Totally, 175
contribution papers (oral and poster presentation) will be presented in this conference and they are
distributed according to the areas previously mentioned. The effor1s of the presenters to prepare their
contribution papers for this conference are highly appreciated.
This Conference is financially supponed by PT Mcrck Indonesia, Olympus, Biorneureux, pT Dipa
Puspa Lab Sain, PT Yakult Indonesia, PT New Moduls Intemational, pT Agar.indo Biological Co, and
parlicipants. Therefore, in this occasion, on behalfofthe committee, I would like to acknowledge their
financial supports.
My thanks should also go to all people who have been involved in the committee ofthe conference.
Without their hard working and effofts, I am afraid rve will not be able to make this event to happen.
Last but not least, I hope you all can enjoy your time in Bali, not only at the venue ofthe conference,
but also enjoy the beauty of Bali and the friendliness of the people, so that you all bring home some
unforgettable memories about the island ofBali.
Thank youl
Chairman of the Organizing Committee
Dr. dr. Dewa Made Sukrama, M.Si., Sp.MK.
Bali - lndonesial22'd -
24'h
Jtne 20111v
The
4th
lnternalional Sehinar of lndonesia Society Jot Mictobiotop and IUMS-ISM Outreach Progan on Food Salea
''lndonesiah Micrcbial Resources: Diversity and Gtobat
5g
lnpaa W
WELCOMING SPEECH BY RECTOR
OF UDAYANA UNIVERSITY
Dear Distinguished guests, Invited speakers, and all other participants. The main aim of this 4th
International Seminar of Indonesian Society for Microbiology and IUMSJSM outreach Program in Food
Safety is to gather scientists from all over the world in a venue to share their expertise in microbiologyreJated discipline and build scientific network, so that they can develop microbiological-based ntethods for
improving thc quality ofhuman Iife in the future.
In this opportunity, on behalf of the University, I welcome you a! to Bali. Bali is well known as a
favorite tourist destination in the world. Recently, it is also a favorite site for holding International events,
such as International Conference. When people hear Bali as a site of an International conference, a lot
olthem will be interested to attend the event. By attending such an event in Bali, they can do two things
at once. Tlley can present scientific papers and share their expedise with other scientists known to have
International reputation, and at the same time they can also enjoy the beauty ofthe Bali Island and the
culture ofBali which is considered to be unique by foreign tourists. Here, I would also like to acknowledge
the National and Intemational invited speakers lor their willingness to come miles away to Bali and present
their high standard papers. I understand that you all spend much time for this conference, and therefore
I must giYe high appreciation on all of those effort and dedication. I hope this International Conlerence
becoines an ideal forura for communication and sharing ideas as well as experience in microbiologicalrelated disciplines in the future. I also hope that this forum can serve as a forum for prolnoting advanced
microbiology with regard to economic growth and social welfare.
Finally, I wish you most successful conference and hope that it may provide new ideas arrd strategics
for the application ofmicrobiology in the industries.
Rector ofthe Udayana University,
Prof. Dr.
dr I Made Bakta, Sp.PD (KHOM).
Bali - Indonesiq | 22' - 21"' Jurc )01
I I vii
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OPENING REMARK OF THE PRESIDENT
OF THE INDONESIAN SOCIETY
FOR MICROBIOLOGY
The Indonesian Society for Microbiology isproud to presentthe4'hlntemational Seminaroflndonesian
Society for Microbiology, in June 22-23, 201I with the theme "lndonesian Microbial Resources: Diversity
and Glqbal Inrpact". I am also very proud to inforrr you that the Intemalional Union of Microbiological
Societies (IUMS) in collaboration with the lndonesian Society of Microbiology(lSM), the International
Commission on Food Mycology (tCFM) and the International Committee on Food Microbiology and
H1'giene (ICFHM) is also organizing an IUMS-Outreach Plogram in Food Safety on 22-24 June 20ll.l
have the great pleasure in welcoming all ofyou to these events.
Over the past decades, there has been impressive progress in the area ofmicrobiology. Microbiology
and biotechnology have been playing important roles in the area ofmedical and veterinary, food and industry,
agriculture and environrrent. This seminar will be an excelient event for exchange and sharing information,
progress and experiences artong the pafiicipants and more irnportanlly to encourage collaboration and
business interaction anong parlicipants. The workshop on food safety is also very impoftant especially for
countries like Asia because transfer ofknowledge I believe will be very useful
The Indonesian Society for Microbiology, a member of the Intemational Union of Microbiological
Societies (IUMS), is a scientific organization in rnicrobiology, with 1067 members spreads out in 20 regions
in Indonesia. ISM has also published an English peer-reviewed journal namely Microbiologt Indonesia,
t|at has been given the accreditation at level "A" by Department ofNational Education, since 2001. The
journal was published in Bahasq Indonesia from 1999-2006 but later on become English journal. I do
expect that the journal rvill become a real international journal. In this regards, I would like to invite all of
you to subrnit your manuscript and give contribution as editor or reviewet of the Microbiolog) Indonesia
stating from 2012.
Tlre society also conducts Annual Meeting rvhich comprises ofscientific meeting both national alld
international, and take place in the society b|anch all over Indonesia. Papers presented in the annual meeting
are usually selected and published in rhe journal Microbiology Indonesia. In this regards, the society would
like to thank the Department ofHigher Education for granting a financial support in collecting appropriate
and acceptable papers to be publish in the journal.
still have to intensifo and extend on the netwolking with international communities. lndonesia
a large dive$ity in microbes, we therefore invite our colleague from abroad to explore
ofthe Indonesian nriclobes based on benefit sha ng.
We
being privilege with
On behalfofthe Indonesian Society for Microbiology,I would liketo express oursincere appreciation
and gratitude to the International Union of Microbiological Societies (IUMS), the International Commission
on Food Mycology (ICFM) and the International Committee on Food Microbiology and Hygiene 0CFMH).
We would also like to extend our appreciation to Udayana Universiry research institution, private sector
and all supporting parties for the success ofthe seminar and workshop on food safety.
Bali - Indonesia | 22'd - 24'h June 2011
lix
kfmlPnq!S?linar ollndonesia
Tef
"Indorresian Microbial Resoxrces:
Societylor Mictobiolog and IUMS_ISM Outeach progam
DiversiO2 ahd Global
lnqdcl"
on Food
SajeE
I would like to extend our appreciation to invited speaker and guest from
foreign country. I believed
your great contribution will be very fruitfur and provide significant
iole in deveroping
its application in these region.
special thanks are addressed to the organizing committee chaired
excellent effort to conduct this successful seminar.
'ricriiorogy
and
by prof Dewa Sukrama his
I do hope that this international seminar and workshop will strengthen our collaboration
in exploring
the role and application ofmicrobiology.
With warm regards,
Dr. Koesnandar, M.Eng
President ofthe Indonesian Society for Microbiology
x
I
Bali - Indonesia | 22d - 24th June
20
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OPENING REMARK OF THE SECRETARY GENERAL
OF THE IUMS
The INTERNATIONAL UNION OF MICROBIOLOGICAL SOCIETIES (IUMS) is one of the 26
Scientific Unions ofthe International Council ofScience (ICSU). It was founded in 1927 as the Intemational
Society of Microbiology, and became the Intemational Association of Microbiological Societies affiliated
to the International Union ofBiological Sciences (IUBS) as a Division in 1967. It acquired independence
in 1980 a"nd became a Union Member of ICSU in 1982. IUMS has 109 member societies and 16 associate
members, altogether witi more than 60.000 microbiologists.
The Divisions are responsible for the organization of their International Congresses (lntematicnal
Congress ofBacteriology andApplied Microbiology,International Congress ofMycology, and International
Congress ofVirology) and the comrnittees, courmissions and federations organize their own meetings. The
next IUMS congresses will be in Sapporo Japan frorn 6 to 16 September 2011 (http:/ yww.congre.cojp/
iums20l I sapporo/index.html )
.
The scientific activities of tlre Union are conducted by the three Divisions natnely Bacteriology &
Applied Microbiology (BAM), Mycology and Virclogy, by six specialist intemational comrnittees, eight
international commissions and two international federations (COMCOFs). Their major activities include
the classification ard notrenclature ol bacteria, fungi and viruses, food microbiology, rnedical nticrobiology
and diagnostics, culture collections, education, and biological standardization.
In support of its mission to enhanse the scieniific background and professional elfectiveness of
basic and applied microbiologists, the IUMS is embarkirg on a progmm of educational outreach to
developing counries and their microbiologists. The Union envisions an IUMS selies of courses that will
be offered to groups of microbiologists that may include graduate students, postdoctoral fellows, and
practicing professionals from developing countries within a given geographic region. These will be offered
periodically in various regions and on diflerent topics ofinterest and importance.
The first IUMS Regional Course was offered in Singapore during June l5- 17, 2010 on Antimicrobial
Resistance in Bacteria, Fungi and Mruses and was great success. The IUMS is very happy that the second
IUMS outreach programme could be organized together with the Indonesian Society of Microbiology
on the topic of Food safety. The International Commission on Food Mycology (Mycology Division) and
International Committee on Food Microbiology and Hygiene (Bacterioiogy and Applied Microbiology
Division) kindly sponsored the workshop by providing the experts on food miuobiology.
On behalfofthe IUMS Executive Board I like to thank the ISM and the local organizers to make this
meeting possible and wish you all a very successlul and productive workshop.
Robert A Samson - IUMS Secretary General.
Bali - Indonesia | 22d - 24'h June
20ll I xi
The 4b lklernational
Outeach Progrom on Food salely
seflinat ol lndonesia societyfor
' " Mitobiolog and IIJMLISM
"lndonesii Microbial
P'esources: DitYrsity ond Global
Inpact"
TABLE OF CONTENT
Preface ftom the chairman ofthe organizing committee
Welcoming speech by Rector ofUdayana University
Opening remark ofthe President ofthe Indonesian Society for Microbiology
vii
ix
xi
Opening remark ofthe Secretary General ofthe IUMS
Table olContent...
xiii
PLENARY SYMPOSIU]U ES)
I.
2.
RECENT DEVELOPMENTS IN THE TAXONOMY OF IMPO:RTANT FUNGI
IN APPLIED MYCOLOGY
R.obert A Samson .
PGPR: NEW POTENTIAL IN HELPING PLANTS IN STRESS CONDITIONS
Panlada Tittabutr, Nantakorn Boonkerd and Neung Teaumroong
3.
4.
5.
""""""'"""
-
"
BOTULISM; MECHANISM, DIAGNOSIS, AND TREATMENT
Keiii Oguma, Yurniko Yamamoto, Tornonori Suzuki, Shaobo M4 Kai Zhang'
Ni Nengah Dwi Fatrnawati, Toshihiro Watanabe, and Tohru Ohyama "" ""'
TECHNOLOCY ]NNOVATION FOR MICROBIAL BASED INDUSTRY
Bustanul Arifin.....
NONCULIURABLE MICROBES: PRESENT STATUS, TECHNIQUE
AND POSSIBLE INDUSTRIAL APPLICATIONS
Fusao Tomita, Ph.D
6.
DIET AND INTESTINAL MICROBIOTA PROFILE
Yuan--Kun LEE and Yong Loo Lin
7.
8,
THELINUSUALENTEROAGGREGATIVEVEROCYTOTOXIN-PRODUCING
ESCHENCHIA COI1O104:H4 AND THE 2011 OUTBREAK IN GERMANY
Kalliopi Rantsiou and Luca Cocolin...
ETHANOL PRODUCTION FROM CELLULOSIC BIOMASS
9.
.
.. .
.
.
Minoru Genta.......
' '
OF INDUSTRIAL ENZYMES IN INDONESIA
APPLICAIION
RESEARCH AND
. . . . .
i :
. . .
. . .
.
.
l0
.
l1
Witono Basuki .....
10.
RAPID MICROBIOLOGICAL METHODS FOCUSING IN ENUMERATION
(QUANTITATIVE) TESTS
Hui Chng Khoo....
t2
SYMPOSIUM PRESENTATION: FOOD AND AGRICULTURE (SFA)
I. ENHANCING NITROGEN AND PHOSPHATE FIXATION BY USING
POTENTIAL MICROBES TO WORK FOR SMALLHOLDER FARMER
IN INDONESIA.
2.
Harmastini Sukiman, Sylvia Lekatompessy, Rumella Simarmata, Tiwit Widorvati'
Liseu Nurjanah and Nuriyanah '.........
AGRICUIURAL ECOSYSTEM MANIPULAIION TO BELOW GROUND
l5
MlCROBIAL DIVERSITY
16
Irfan D. Prijambada .."..........'.. .........
BqIi - Intlonesia | 22'd -
24'h
June 2011
lxiii
me
4h
Inlemational sehiur of Indondia societyJot Mi*obiolog and TIJMS-IsM ouueach progran on Food safeE
Microbial Resources: Dhersity and Globat Inpacl "
" Indanesian
3.
BIOINDUCTIONTECHNOLOCYINCREASEDGAHARU.FORMING
II] AZUIL,INA AND GTRINOPS BY FUSANUM SPP.
4.
Maman Turjaman, Erdy Santoso, Ragil SB lrianto, Irnayuli R. Sitepu................................. 17
POTENTIAL OFRESOURCE OF STARCH AND LOCAL ISOLATES
MICROORGANISMS FOR PRODUCING DRUCS-EXCIPIENT TO
5.
STRENGTHEN OF INDONESIAN PHARMACEUTICAL INDUSTRY
Mulyono, Hade Sastra Wiyana, and Dian Herasari .
UTILIZATION OF CENOMIC INFORMATION ERAAND INDONESIA
MICROBIAL DIVERSITY TO IMPROVE THE LOCAL INDUSTRIAL
..
l8
BACTERIAL STRAIN
Is
6.
He1ianti.............
lg
BIOCONTROL POTENTIAL OF MICROBIAL ANTAGONISTS AGAINST
PLANT FUNGAL PATHOCENS
Dewa Ngurah
Suprapta......................
SYMPOSIUM PRESENTATION: HEALTH AND PHARMAcY (SHP)
1. RABIES IN BALI : CLINICALASPECT AND LABORATORY
Sudewi AAR, Susilawathi NM, Darwiaata AE, Dwija P, Budayanti NS,
Mahardika GNK
2. CURRENT PROCRESS IN T}IE LABORATORY DIAGNOSIS
OF JEMBRANA DISEASE IN BALI CATTLE
20
23
Nyoman Mantik Astawa
PATHOGENESIS OF DIARRHEA CAUSE BY ROTAVIRIJS INFECTION
3.
A.T.Aman.-----.
SYI}IPOSIUM PRESENTATION: ENVIRONMENTAND INDUSTRY (SEI)
1. THE ROLE OF LACTIC ACID BACTERIA IN TEMP OYAK FERMENTATION,
AN INDONESIAN INDIGENOUS FERMENTED FOOD
Rindit Pambayun
2. THE ROLE OF LACTIC ACID BACTERIA ON FERMENTATION OF URU?7N,
BALINESE FERMENTED SAUSAGE
3.
4.
29
Nvoman Semadi Antara
30
PRODUCTIONANDCHARACTERIZATIONOFNANO.SIZEI)
EXOPOLYSACCHARIDE OF HUMAN FECAL LACTOBACIZ'US SP. FUOsI I
Acturad Dinoto and Sugiyono Saputra
BIOPRESERVATIVES OF LACTIC ACID BACTERIAL ORICIN :
UNCOVERING GASSERICIN A OPERON, BACTERIOCIN GENES FROM
3l
LAC TO BACILLUS GASS ERI L A39.
5.
xw
l
Joni Kusnadi
32
DEVELOPMENT OF MICROBIAL RESOURCE CENTER IN INDONESIA
Puspita Lisdiyanti, Atit Kanti, I Made Sudiana, Hiroko Kawasaki, Achmad Dinoto,
Shigeto Otsuka, Moriya Ohkuma, Ken-ichiro Suzuki, and Heddy Julistiono
33
Bali - Indonesia | 22'd - 24,h June
20ll
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ORAL PRESENTATION: FOOD AND AGRICULTURE (OFA)
1. DETECTION OF SAL]UIONELLA TYPHIMURIUM IN PASTEURIZED MILK
AND FRIED RICE USING REAL TIME POLYMERASE CHAIN REACTION
SuciYuliangsih,KhusnulKhotimah,NoviPusparini,RiolinaldaLamtiurPanggabean
and Winiaii Pudji Rahayu....................
2. ANTIMICROBIALACTIVITY FROM MELNJO SEEDAND PEEL EXTRACT
(GNETUM GNEMON L.) AGAINST PATHOGENIC BACTERIA
3.
37
44
Adolf JN. ParhusiP
MOLECULARANALYSIS OF SUCCESSION OF BACTERIALAND T4'
BACTERIOPHAGE COMMUNITIES DURINC THE COMPOSTN'IG
OF RICE STRAW
-
Vita Ratri
4.
5.
6.
7.
Cahyani
54
PRESERVATION OF RIBBON FISH (TNCHIURUS LEPTURUS) USING LACTIC
ACID BACTERIA CULIURED ISOLATED FROM WILD HORSE MILK
I Made Sugitha, Deprilia Eka Dewat4 Ni Nyornan Puspawati." """" """
DEVELOPMENT OF IN PLANTA TRANSFORMATION OF RICE
6l
Suparthana I Putu, Masahiro Nogawa and Mineo
62
"""
Kojirna
INDUCED APOPTOSIS IN T47D CELL LINE BYPUREACTIVE COMPOUND
FR]M EMENCELLA N(DULANS MARINE FUNCUS
Muhammad Nursid, Ekowati Chasanah, Murwantoko, and Subagus Wahyuono "" "
ISOLATION AND SELECTION OF AUXIN-PRODUCING BACTERIA
FROM RHIZOSPHERE OF SELECTED PLANTS
8.
Bambang Sukmadi, Yenni Bakhtiaq Farida P.osana Mira and Bedah Rupaedah ""
SCREENING OF INDIGENOUS BACTERIAAS THE SUPPRESSING ACENT
9.
OF RICE FUNGAL PATHOGEN NHIZOCTONIA SOLANI
" """
Yacli Suryadi, Kartika Eka Putri, Nisa Rachmania Mubarik
POPULATION
TO
STIMULATE
COMPOSTS
EFFECTIVITY OF VARIOUS
10.
11.
I3.
63
""" " "
64
""
"""""
65
OF NITROGEN- FIXING BACTERIA, PHOSPHATE-SOLUB]LIZING
BACTERIAAND INDOL ACETIC ACID PRODUCTION
Yulia Nuraini, Abdul LatiefAbadi, Soemarno and Tri Ardyati """"" """"'
ASSESSMENT OF ANTIBIOTIC RESISTANCE FROM LACTIC ACID BACTERIA
IN INDONESIAN FERMENTED FOODS FOR SELECTABLE MARKER
TO DEVELOP FOOD-GRADE VECTOR
A. Zaenal Mustopa, Urnemi, Mariclha Nomawati and Ikrimah Muzdalifah """"""""
EFFECT OF FERMENTATION OF PALM KERNEL CAKE MIXTURE - RICE
BRAN WITH TNCHODERMA VINDE ON SUBSTANCES FOOD INGREDIENTS
Sukaryana Y., U. Atmomarsono, V.D. yunianto, E.
12.
'
""
Supdyatna"""""
66
"
'
"'
67
'
68
USAGE OF CARAGENAN ('UCflE UMA SPINOSUM AS LACTOBACILLUS
AC IDOP HILUS ENCAPSULATED MATERIAL TO!!/ARD VIABILITYAND
MICROCAPSULES STRUCTURE TINDER IN VITRO GI TRACT PH SIMULATION
69
Dwi Setijawati
INHIBITION OF METHANOL EXTRACT OF JACKFRUIT TI-R''OCARPUS
INTEGM MERR) WOOD ON THE GROWTH OF SPOILAGE MICROBES
IN THE PALM SAP DURING STORAGE
'70
...........
14.
I Nengah Kencana Pu1ra.........
STUDY OF PROBTOTIC PORRIDGE FLOUR FOR EMERGENCY FOOD:
TYPES OF FLOUR AND LAB CONCENTRATION
"" ""
Dian Widya Ningtyas, Simon B Wi janarko.
.........
"""
"""
""
Bali - lndonesia | 22d - 24'h June 201I
7l
lxv
The 4,r hternational Seltinar ollndonesia SocieirJor
"l
Micrcbiolog ond IUMSJSM Oureach Progran on Food Safety
donesian Microbial Resaurces: Di,elsily and Global
lnpact"
I5.
ISOLATION AND CHARACTERIZATIOI'I OF PLANTARICIN GENES
16.
LACTOBACILLUS P LANTARUII[ FROM INDICENOUS INDONESIAN
TRADITIONAL FOOD FOR BIOPRESERVI\TIVES APPLICATION
Linda Sukrnarini, A. Zaenal Mustopa, Muhamad Ridwan, and Reni Nurhaeni
STABILITY OF I-ACTIC ACID BACTERIA DURING PROCESSINC
AND STORACE
17,
18.
OI-'
72
GOAI MILK SOFT CHEESE
Widya Eka Prayitno, Feri Kusuandar, and Winiati Pudji Rahayu .. ....
ENDOPHYTIC FUNCI FROM IG'I IA HAfuUISIANA AND ISOLATION
OF BIOACTIVE COMPOLIND
Vithria Nida, RizkiAprilianti, Elrade Rofaani and Chaidir....................
PROCESS DEVELOPMENT OF FERMENTED CASSAVA FLOUR:
T}IE EFFECT OF STREPTOMYCES CELLULOLYTIC BACTERIA ADDITION
Nurwina Eka Putri, Titi Candra Sunarti,lnja
"
13
74
Meryandini-
75
ORAL PRESENTATION: FIEALTH AND PHARMACY (OHP)
]
.
,I-2. '
I
PROBIOTIC ENTEROCCI: CURRENT STATUS
Agus Wijaya.........
,
.;
J.
cuenacrERIZArIoN oF SAMqNELLA ryPEllsoLATEs
PROFILES
Amarantini
6.
7.
)
:8J
i
Charis
CON1PARISON DETWLEN AC IIVI TY AND MECI-IANISM OF
OF ESSENTIAL OIL BETEL LEAF (PIPER BETLE. LINN) WITH EUGENOL
AGAINST SOME OF BACTERIAL PATHOGENS
Muhammad Yanis Musdja, Amir Syaril, Emie Hernawati Poerrvaningsih
II'IHIDITION
"'/
Agusta
90
DOES PIPER BEZZE LIN}.I LEAF CAN INHIBIT THE GROWTH
OF SALMONELLA T''PI11AND YIBNO CHOLERAE?
Oksfriani Jufri
5.
BY UsING
NUUTNICAL SYSTEMATIC ANALYSIS OF THEIR SDS-PAGE PROTEIN
and Andria
4.
'79
Sumamportw................
100
BACTERIOLOGYAND IN VITRO ANTIBIOTIC ACTIVITY OF DIABETIC
FOOT INFECTIONS IN UL]N GENERAL I{OPITAL BANJARI.4ASIN ,
BANJARBARU CENERAL HOSPITAL, AND RATU ZALECHA GERAL
HOSPITAL MARTAPURA
Ra1uriati...............
104
CLONING AND EXPRESSION OF SMALL ACCESSORY T,N 7 GENE
OF JEN4BRANA DISEASE VIRUS
Endang Tri Margawati, Indriawati and Muhamad Ridwan ................... ..
105
VIABILITYAND PENICILLIN-G REPRODUCIBILITY OF PENICILLIUM
CHRYSOGENUM PRESERVED ON VACUUM DRIED-QUARTZ POWDER AFTER
A PERIOD OF 16 AND 17 YEARS STORAGE
Uli Julia Nasution, Dian Japany Puspitasari, Diana Dewi, Gany Heriyanto,
Hardaning Pranamuda, Bambang Marwoto
8.
9.
....................
GENOTYPE ANALYSIS OFDENGUE VIRUSES IN JAKARTA
T. Mirawati Sudiro, Beti E Dewi, Rony Chandra, Rayhana, L Nainggolan, M Karyanti,
Angky Budianty, Dwi Hilda, Dimas Seto, Wellyzar, Mustaqim, Novia, I. Kurane........
PREVALENCE OF HEPATITIS E VIRUS AMONG HUMANS AND PIGS IN BALI
Takako Utsumi, Yoshitake Hayashi, Maria Inge Lusida, Mochamad Amin, Soetjipto,
Made Agus Hendrayana, Soediningsih, Yoshihiko Yano, Hak Hotta........... .
xvi I Bali- lndonesiq
22'd - 24'h June
20ll
106
'
107
i 08
The 4h lnternational Seminar oJlndonesia Socielyfor Mictobiolog) ald IUMSJSM Outreach Program on Food SaJeE
"lndo esian Microbial Resources: Diversily afid Global Inpact"
I
O.
EFFECT OF TEMPERA'TUR VARIAIION AND PH TO THE PROTEASE
ACTIVITY OF THERMOPHILIC BACTERIUM SPECIES FROM LEJJA HOT
SPRINGS, SOUTH CELEBES
MohamadAmin, Hafsah, ard
I
I.
12.
1
3.
I
4.
YusufAbdurrajak
109
DETECTION OF ANTIBODIES OF AVIAN INFLUENZA VIRUS TO H5N1
SUBTYPE IN PIGEONS IN BANDAACEH
Muhammad Daud Aiyub Kadir, Erin4 and Mahdi Abrar....
MIXED CULTURE OF ENTEROCOCCUS FAECALIS ID6O17 AND
C HRYSEOBACTENUM INDOLOGENES ID6O16: DETERMINATION OF BEST
DECOLORIZATION OF ORANCE II BY MIXED CULTURE OF C.
INDOLOGENES AND E. FAECALIS ON ORANGE II CONTAINING MEDIUM
Vincentia lrene Meitiniarti, Kris Herawan Tiraotius, Endang Sutariningsih Soetarto,
I
METHICILLIN RES I STANT S1U PIfl'Z O C O C C US A U KEII,S(MRSA)
IN SANGLAH HOSPITAL: DISTRIBUTION AND ANTIMICROBIAL
SUSCEPTIBILITY
Sukrama D.M, Tarini M.A, Darwinata A.E .......................
ANTIBACTERIAL POTENTIAL OF EXTRACELLULAR METABOLITES
PRODUCED BY.BI CLLLUS SP. SYMBIONTS OF THE SEA URCHIN
ll2
Hidayati......................
I5.
16.
17.
I8.
113
Bambang Fajar Suryadi and Ernin
MOLECULAR ANALYSIS OF HUMAN METAPNEUMOVIRUS 42,
INFLUENZA A VIRUS H3N2 AND INFLUENZA B VIRUS ISOLATED
IN SURAKARTA INDONESIA
Afioflo Agung Prasetyo, Jinn]y Tanamas, Maftinus Nuhenvan Desyardi, Luqma Prilata,
...
Maryani, Suradi, Harsini, Seiji Kageyarna, Hiroki Chikumi................
PRODUCTION OF POLYCLONALANTIBODY FROM THE DENGUE VIRUS
TYPE 3 NON.STRUCTURAL I PROTEIN
Vanny Narita, Aris Rudiyanto, Asma Ornaq Mira T. Sudiro ..........'........
IDENTIFICATiON OF CTXA GENE BY PCR METHOD FP(OM YIBNO
CHOLEM BACTERIA ON ICE FROM FISH TRADERAND FISHERMAN
IN SOUTH BADUNC REGENCY AREAS AND DENPASAR CITY
Made Agus Hendrayana, Ni Made Adi Tarini, Agus Eka Darwinata...............'
PRODUCTION AND SCREENING OF HYBRIDOMA CLONES PRODUCING
MONOCLONALANTIBODIES SPECIFIC TO PROTEIN NON.STRUCTURAL
1 (NS1) DENGUE VIRUS SEROTIPE 3
Vanny Narita, Aris Rudiyanto, Mira T Sudiro, Beti Ernawati Dewi,
Mustaqim
ORAL PRESENTATION: INDUSTRY AND ENVIRONMENT (OIE)
DETECTION OF CELLULOLYTIC ACTIVITY AMONG CELLULOLYTIC
I.
BACTERIA ISOLATED FROM RICE FIELD AND COW DLING
Tri Ardyati, Triani Agustin, Eva Rinayanti W. and Suharjono
2.
l0
I
l5
l16
1l'1
...
.............
114
l2l
STUDYABOUTTHEHEXAVALENTCHROMIUMREDUCTIONCAPABILITY
BY RHIZOBACTERIA ISOLATED FROM SOIL CONTAMINATED CHROMIUM
M.M.A. Retno Rosariastuti Ali Pramono, Angry Rosa Nusa Putri, Ngadiman
and lrfan Dwidya
3.
Prijambada..............
126
IN YITRO ADHESION OF LACTOBACILLUS SPP ON MOUSE ENTEROCYTES
I Nengah Sujaya, Yan Ramona, A. Eka Suryadarma, NP Desy Aryantini,
K.A.
Nocianitri....
123
Bali - Indonesia | 22'd -
24th June
2011 xvii
The 4k Internatiohal Seminar oflhdohesia Socieryfor
Micrcbiologand IUMSJSM Outeoch Prugram
Inpacl"
on Food SaJety
"Indohesian Mictobial Resources: Di.Etsity ahd Global
4.
IMMOBILIZED SHINGOMONAS SUBARCTICA T7B IN POLYVINYL
ALCOHOL AND SODIUM ALGINATE FOR DIBENZOTHIOPHENE
BIODESULFURIZAIION
5.
6.
Asano............................
Ida Bagus \Yayan Gunam, Teruo Sone & Kozo
FERTILIZER DOSE EFFECT ON DENSITYAND POTENTIAL OF MICROALGAE
(NAANNOC HLOROP SIS OCUI-ATA) AS BIOFUEL
Cenny Putnarubun, Marwa, Wawang Suratno, Poniah
EFFECT OF COMPOSTADDITION ON BIOREMEDIATION OF OIL-
Adyaningsih...............
137
143
CONTAMINATED SOIL FROM JAMBI BY COMMERCIAL BACTERIA
7.
Suhardi.....
Rohana Udur Hutabarat, Gladys Setyamukti, Sri Harjati
CHLORINE DISINFECTANT ACTIVITIES TO ESCHENCU COU,
P S EUD O MONAS AEfi UGINOSI, AND SALMONELLA TYP}/1 ISOLATES
FROM MARTAPURA HOSPITAL'S WASTE WATER
Lia Yulia Budiarti,
8.
9.
I
O.
Rahrniati...............
152
153
SCREENING AND APPLICATION OF SOIL BACTERIA ISOLATED FROM
PEATLAND ON GROWTH OF ACACIA CMSSICARPA
Nisa Rachmania Mubarik, Ade Wiratarna, Melisda Manalu, Lailan
154
BIOPROSPEK LOVASTATIN OF ASPERGILLUS SPP FROM UNI\'ERSITY
OF INDONESIA CULTURE COLLECTION (UICC)
Wibol,o Mangunryadoyo, Yanti Rafliyalti and Dadang Kusmana...................................... 155
POTENTIAL USE OF WOOD FIBER WASTE AND FISH WASTE COMPOST
AS SUBSTRATES FOR THE I,OW.COST CULTIVATION OF BIOLOGICAL
Syauflna.
CONTROL ACENTS
Yan Ramona and Martin A.
1
I.
12.
I3.
14.
METHANE OXIDATION AND AMMONIUM ACCUMULATION ACTIVITY,
AND CHARACTERIZATION OF,Ir'lFD AND N1F'I] GENES FROM
METHANOTROPHIC BACTERIA
Ari Fina Bintarti, Iman Rusmana.......
MICROBIAL TECHNIQUE IN REDUCI}.IG CREENHOUSE GAS EMISSIONS
FROM WETLAND SOILS
Abdul Hadi, Zuraida Titin Maryana, and Aprianti
SCREENING AND GENETIC DIVERSITY OF SPONCES ASSOCIATED
BACTERIA PRODUCING WIDE-SPECTRUM ANTIMICROBIAL COMPOUNDS
Aris Tri Wahyudi, Rika lndriAstuti, Yonathan Banoet, Sri Budiarti, Pauliasi Tokasaya,
Hermawaty Abubakar, Munti Yuhana , Masafurri Yohda.
PROPERTIES OF THERMOSTABLE EXTRACELLULAR AMYLOLYTIC
ENZYME OF BACILZT,/,S SP SW-2 ISOLATED FROM SERPONC COMPOSTING
UNIT, WEST JAVA
Budiasih-Wahyuntari,
I5.
I6.
Line.........
Trismilah
156
l5'1
158
159
160
PROPERTIES OF EXTRACELLULAR PROTEASE OF BACILLUS MEGATEKIUM
DSM 3I9 AS UNHAIRING AID OF HIDES
Budiasih Wahyuntari, Hendrawati, Aldi Sadana, Rarah RatihAdjie, M Yamin.................... 161
THE UTILIZAIION OF THE RESPONSE SURFACE METHODOLOGY
FOR THE OPTIMIZAIION OF CULTIVAT]ON MEDIUM OF S''REPTOMYCES
sPAl I ON CYCLO(TYROSYL-PROLYL) PRODUCTTON
Rofiq Sunaryanto, Bambang Marwoto, Tun Teja Irawadi. Zainal Alirn Mas'ud,
Liesbetini Hartoto
.................
xvni I Bali - Indonesia | 2?'d - 21t1' June
2011
162
me4
17
I
-
.
8.
Internatiorutseminarortntto
esiasocietyr,y;";;l,l;7#::f,yt;::y:::,,";:l::;",:;;ii:ii:;{?
ROLE OF ENDOPHYTIC ACTINOMYCETES INDOLE ACETIC ACID IN
PROMOTING THE GROWTII OF RICE PLANT
Yulin Lestari, Tera Tri Yusepi, I{arnim
D]VERSITY AND ACTIVITY OF AMMONIA OXIDISERS IN INDONESIAN
PINE FOREST SOILS
Rully Acli Nugroho and Wilfred F.M' Rdling ....................'
POSTER PRESENTATION: FOOD AND AGRICULTURE (PFA)
I. ANALYSIS ON THE SPORULATION CAPACITY OF BACILLUS CEREUS
STRAINS
Zahirotul Hikmah tlassan, Menno Van Der Voort, Tjakko Abee .'.............. .......
2. THE EFFECT OF LEVAMISOL IN INCREASING INGESTION ACTIVITY OF
MACROPHAGE AGAINST,S. ZOO EP ID EMIC US.
I Nengah Kerta 8esung......................
3. MORPHOLOGICAL AND PHYSIOLOGICAL FEATURES OF
PSEUDOMONAS FL UOR',SCE/r',' P6O
Loekas Soesanto, Endang Mugiastuti, and Ruth Feti
ISOLATION AND SCREENING OF ENDOPHYTIC MICROBES FROII NEEM
AS BIOLOGICAL CONTROL OF PLANT PATHOGENIC FLI'iGAL
Rallayuniati...........
4.
Sukiman......'.........
5.
Rumella Simarmata and Harmastini
ANTIFUNCAL POTENTIAL OF LEAF EXTRACT OF TEAK
(TECTONA G]L4ND1,S L.F) AC AINST ACREMONIUM BUTYNC
Ni Putu Adriani Astiti and Dewa Ngurah
SEROPREVALENCE Q FEVER IN BALI CAITLE (BOS SONDAICUS)
Suprapta.........
6.
@
163
164
167
1"13
l'16
182
189
AND GOATS AT BALI PROVINCE BY INDIRECT IMMUNOFLOURESCENT
ANTIBODY ASSAY METHOD
Hapsari Mahatrni and Agus Setiyono .............
7.
8.
..
195
CASE STUDY: IDENTIFICATION OF SPOILACE MICROORGANISMS ON GEZ1U1(
MO D ERN W IL ING, SEMARANG.INDONESIA
Lindayani, Hendra Wibowo, Budi Widianarko, Laksmi Hartayanie
INDUCTION OF TR]CHOSANTHES CUCUMERINAL.YARANGUINA (L,)
HAINES HAIRY ROOTS USING IGROBACTENUM KHIZOGENES
FOR PRODUCING OF BIOACTIVE PROTEIN
Priyosoeryanto.............
9.
Churiyah, CustafAdolf Wattimena, Bambang Pontjo
'INFECTION LEVEL OF PATHOGEN BACTERIA IN PORK MEAT
IN TRADITIONAL MARKET OF DENPASAR"
Ni Nyoman Puspawati, Komang Ayu Nocianitri, Nyoman Semadi
MICROBIAL FLORA IN THE REPRODUCTIVE TRACT OF LONG.TAILED
Antara........
IO.
MACAQUES (MACACA
1
.
I.
12.
201
206
207
FASC IC ULA PJS)
Aida LT Rompis, I Gede Soma.........
208
COMMUNITIES VARIATION OF PHOSPHATE SOLUBILIZING BACTERIA
IN PLANT RHIZOSPHERE CROWING ON ACID SOILS
Agustian, C. Plassard, D. Maryanti and L. Maira................
209
COMPARISON OF MICROMONOSPORA SPECIES FROM SOILAND
MARINE SOURCES
Yudhie Istianto, Siti Handasah, Fuji Nurul Anami, Satoshi Sugimoto, Yoshio Watanabe,
Hardaning Pranamuda, Bambang Marwoto
.....................
Bali - Indonesial22 - 24't'June 2011
210
lxix
The
4th
Interhatiorul seminar ollndonesid socieyJor Miclobiotog)and |\MS-ISM ot)treach pngram on Food safety
"lndo esia Miuobial
13.
14,
I5.
I6.
17.
I8.
19,
20.
21.
P,esoutces:
Diwrsity and Global Impact"
DETECTION OF PORK IN BEEF CORNED FOR HALAI, VERIFICAIION VIA
POLYMERASE CHAIN REACTION (PCR) TECHNIQUES
Zilhadia, Wahyu Purbowasito, Yophi Mulyan4 Hamid Nasuhil,Nurrreilis,
Ayu Hapsari
...
Zll
ANTIMICROBIAL ACTIVITY OF Ir'.HEXANE EXTRACT OF AVOCADO SEEDS
(PERSEA AMENCAIr'I MILL) AGAINST FOOD PATHOGENIC AND
SPOILAGE BACTERIA
Eka Putri ..............
THE ABLTNDANCE OF ARBUSCULAR MYCORRHIZAL FUNGI IN
RHIZOSPHERES OF CASHEW PLANTS
2l?
Meitini W
Zl3
Proborini............................-
SELECTION OF BACTERIAL CONSORTIA TO CONTROL BLAST
DISEASEIN RICE PLANTS
Eko Riana, Yadi Suryadi, Nisa Rachmania Mubarik.....
EVALUATION OF FOOD SAFETY PRACTICES OF STREET VENDED CHICKEN PORRTDGEAT SIMPANG LIMA SEMARANG
Inneke Hantoro, Ita Sulistyawati, Melissa Hapsari and Nikita F1ourencia..........................
ANTI FUSANUM O.XYSPORUM ACTIVITY OF SECONDARY METABOLITE
FROM MARINE FUNGI (PR 28.I)
Susi Kusumaningrum, Elrade Rofaani,Agus Supriyono.
INFECTION LEVEL OF PATHOGEN BACTERIA IN PORK MEAT
IN TRADITIONAL MARKET OF DENPASAR
Ni Nyoman Puspawati, Komang A1,u Nocianitri, Nyoman Semadi Antara. ........
PSEUDOMONAS FLUORE.'C'NS AND PSEUDOMONAS PUTIDA
FOR PROMOTING GROWTH OF JATROPHA CUXCI.T SEEDLING ROOT
Sri Sumarsih and Darban Haryrnto....
E}.IHANCING NITROGEN AND PHOSPHATE FIXATION BY USINC
POTENTIAL MICROBES TO WORK FOR SMALLHOLDER FARMER
214
..
?15
216
Zl7
2lg
IN INDONESIA
Harmastini Sukiman, Sylvia I-ekatompessy Tiwit Widowati, Liseu Nurjanah
and
22.
23.
24.
25.
Nuriyanah......
Zlg
ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGE INFECTING
PATHOGENIC BACTERIA: A STRATEGY FOR IMPROVING FOOD SAFETY
Agustin Krisna Wardani, Aji Sutrisno And Zein Nurizkiawan ..........
COW MILK PROTE]NS AS INDONESIAN HERBAL FOOD SYSTEM
Muhamad Sahlan and Tony Supardi...
THE INFLUENCE OF YAKULT CONCENTRAIION AS STARTER ON PROFILE
YOGHURT OF SKIM MILK POWDER
Wisaniyasa, Ni Wayan and Suantari, Ni Luh Wayan.........
OVER-FERMENTED TEMPE (TEMPE BOSOTQ: ITS SENSORYAND
CHEMISTRY PROPERTIES AS FOOD SEASONING
Martina Andriani, Kawiji, Edhi Nurharradi.........................
26.
ANTIFLTNGALACTIV]TY OF GARLIC AGAINST BREAD MOLDS
27.
Jaruwan Yousuk, Tanthip Saetiw,Chonlada Naweewong and paramee Noonirn
INHIBITION OF EXOPROTEASE PRODUCTION IN AEROMONAS
..................
220
Z2l
Z2Z
223
224
HYDROPHIL4'S QUORUM SENSING SYSTEM BY METHANOL EXTRACTS
OF FRESH AND DRIED RHIZOME OF GALANGAL (,4 LPINIA GALANGAL.)
Artini Pangastuti, Rita Rakhmawati, Yashinta Novitasari....
xx
I
Bali - Indonesia | 22d -
24th June 2011
225
The
28.
29.
30.
3
!.
32,
33.
34.
35.
4! lnternational Setninar
oJ
Indonesia Societylor Mi.robiolog) ahd IUMSJSM Ouneach Progran on Food Salety
-lndonesian
Microbial Resources: Diwrsih)dnd Global Impact'
CONCENTRATED JACK FRUIT (IRTOCARPUS INTEGRI, MERR) JUICE AS
FLAVOR COMPONENT IN COCONUT MILK YOGHURT
Maya M Ludong, M F Sumual, G S Suhartati Djarkasi, and Lana E LaIujan....................... 226
INCREASED PRODUCTION OF POLYPHENOL OXIDASE BY
TNCHOTHECIUM.9P, ENDOPHYTIC FUNGUS FROM COCOA PODHUSK,
IN SUBMERGED LIQUID CULTURE
............................... 227
Sarlini, Abd. Rauf Patong,ljodi Harlim, Pirman.....
EFFECTIVENESS BACTERIAL CONSORIIA FOR CONTROLLING LEAF
BLIGHT DISEASE IN RICE PLANTS
Mubarik.....
228
Fadhila Achmad Syachroni, Yadi Suryadi, Nisa Rachmania
LACTOBACILLUS
ISOLATION AND CHARACTERIZATION OF
SPP. FOR
DEVELOPMENT OF SOY MILK BASED PROBIOTIC
K. A. Nocianitri, IDG. Mayun Permana, I N.
.........-.............. 229
ANTI}'UNGAL ACTIVITIES OF STREPTOMYCES SP AGAINST
NUM SP.
THE CAUSE OF LEAF ROT DISEASE ON ALOE BARBADENSIS '{/SI
MILLER
230
Retno Kawuri and Dewa Ngurah Suprapta
QUALITY CHARACTERISTICS C}IANGES OF KUNYITASAM (CUP.CUM4
Sujaya....
..........................
DOMESTICAYAL_ TAAL4NNDUS INDICI L ) DURINC STORACE
... 231
Sri Mulyani, I GA Lani Triani and Bagus Putra Santosa .....................
ASPERGILLUS DIVERSITY IN COFFEE POWDER FROM TRADITIONAL MARKET
IN SEMARANG
Wiwik Andriani, Isrvoro Rukrni & Susiana Purwantisari ....................... ............................... 232
PRODUCTION OF AMYLOGLUCOSIDASE FROM "ONGGOK"
BY ASPERGILLUS NIGER
I WayanArnata, Dwi Setyaningsih, Nur
Richana..........
POSTER PRESENTATION: HEALTH AND PHARMACY (PHP)
I. ADHERENCE AND PHAGOCYTOSIS CAPACITY OF STREPTOCOCCUS
ISOLATED FROM SUBCLINICAL MASTITIS OF DAIRY COW MILK
Agnesia Endang Tri Hastuti
?33
SP.
Wahyuni.
237
2.
POTENCY TEST O}. ANTI-IDIOTIPIC ANTIBODYAS A SAFE RABIES VACCINE
3.
IDENTIFICAIION OF ANTIBACTERIAL COMPOUND OF ZIIr'GIB ER GMMINEUI|,T
BLUME, A BALINESE MEDICINAL HERBS
Sayu Putu Yuni Paryati
4.
5.
6.
lF,\
W9
........................
244
Martiningsih, N.W., Swantara, M.D., Ciawi, Y.................
CONCENTRATION OF 20 % IN THE EXTRACT OF ARECA CATECHUL. MAY
REDUCE THE NUMBER OF COLONIES OF CANDIDA I'BICIN.' ON THE HEAT
CURED ACRYLIC RESIN
249
Ni Kadek Sugianitri...........................
THE BACTERIAL PATTERN IN URINARY TRACT 1NFECTION
AT THE KIDNEY STONE PATIENTS IN DT. SARDJITO HOSPITAL
755
Eka Yudha Rahman, Aries Alpendri, R HR Danarto
CROSS PATHOLOGICAL OBSERVATION OF ACUTE NEWCASTLE DISEASE
260
..........
IN DOMESTIC CHICKEN
GA Yuniati Kencan4 I Made
Kardena
261
Bali - Indonesia | 22d -
24th
June 2011
|
xxi
The
4!^
Iktematiohal Semikat oJlhdonesia Societyfor Mictobiologr' and IUMSISMO treach Progrdm on Food Salety
Microbial Resources: Dirersity and G lobal lnpact"
" Indohesian
7.
DETECTION OF SHIGA-LIKE TOXIN II [SUI-II] OR VEROTOXIN 2 [VT2]
GENE FROM BACTEzuA ESCHERICHIA COLI SEROTYPE OI57 ON PORK IN
DENPASAR CITY
8.
9.
Amy Yelly Kusmawati, Made Agus Hendrayana, Komarg Januartha Putra Pinatih .........
EXOPOLYSACCHARIDE-PRODUCINCBACTERIAISOLATEDFROMFECES
OF HUMAN CONSUMING A LARGE AMOUNT OF SAGO STARCH IN DIET
Sugiyono Saputra, Rita Dwi Rahayu, And Achmad Dinoto................
BLACKBERRY PLANT EXTRACT DECREASES NECROTIC AND APOPTOTIC
CELLS AGAINST OXIDATIVE STRESS
Badrut
IO.
I1.
12.
13.
...
262
263
Tamam......
264
MICROBIAL FLORA IN THE REPRODUCTIVE TRACT OF LONG.TAILED
MACAQUES (MACAC A FA S C TC U LA N S)
Rompis All, I Gede Soma .........
METHICILLIN RESISTANT S77PI1TZ OCOCCUS AUREUS(MRSA) IN SANGLAH
HOSPITAL: DISTRIBUTION AND ANTIMICROBIAL SUSCEPTIBILITY
Sukama D"M, Tarini M.A, Darwinata A.E.....................
SENSITIVITY AND SPESIVISITY PILI ANTIGEN PSEUDOMONAS AERUGINOSA
FOR DIAGNOSIS OF PNEUMONIA BY USING DOTBIOT METHOD
Febri Kurniawati , Maryani, Yusup Subagio Sutanto dan Priyarnbodo.......
EVALUATION OF PARTICLE SIZE AND ZETA POTENTIAL OF DNA
CONDENSATION WITH LIPOPEPTIDE - BASED TRANSFECTION REAGENTS
FOR EFFICIENT CENE DELIVERY
Tarwadi, Jalal A. Jazayeri, Richard J. Prankerd, and Colin W. Pouton...
..
265
266
267
268
POSTER PRESENTATION: INDUSTRY AND ENVIRENMENT (PIE)
I. PRODUCTION OF NATURAL COLORINGS FROM MONASCUS PURPUREUS
AND P ENICILLIUM PURP UROGENUM: DETERMINATION OF CONDITIONS
FOR OPTIMUM YIELD AND STABILITY OF THE COLORINGS
Anastasia Prirna Kristijarti, Ariestya Arlene, Astri Puspaningrurn Nugroho,
Cathelya
2.
Kahar
3.
4.
Anglelyn
Muzakhar..
Dannayasa................
283
288
OP'I]MIZATION OF PH, TEMPERATURE AND AGIT,{TION RATE OF
BIODEGRADATION OF LIPIDS AND DETERGENTS IN LIQUID FOOD WASTE
BY BACILLUS CEREUS N.09.
Nur Hidayat, Sri Kumalaningsih, Noorhamdani and Susinggih
6.
277
BIODIVERSITY OF ENDOMYCORRHIZAE FROM HATTA GRAND PARK
AND ANAI VALLEY ARBORETUM GARDEN PADANG, WEST SUMAIERA
INDONESIA
Sylvia Lekatompessy, Harmastini Sukiman and Kartini K-ramadibrata ....
ISOLATION AND IDENTIFICATION OF SOLUBLE PHOSPHATE BACTERIA
IN SEDIMENT OF' TUKAD BADUNC ESTUARY DAM AND BTDC NUSA DUA
BALI V/ASTE PROCESSING LAGOON
Ida Bagus Gede
5.
271
VINASSE BASED MEDIUM FOR EXOXYLANASE PRODUCTION
Wijana..
PURIFICATIONANDBIOLOGICALACTIVTTIESOFANTIBACTERIAL
ANTIBIOTIC PRODUCED BY STREPTOMYCES SP BIOMCC AE-OO1I5
Sasmito Wulyoadi, Wahono Sumaryono and Wahyudi P Suwarso.................
xxii ) Bali - htdonesiq | 22"d - 24th June 201l
291
296
The
7.
41h
lnternalional Seminor oflndonesia SocietyJor Microbiolog) and IUMSJSM Outreach Progran on Food SqIery
"Indonesian Microbial Resoutces: DiversiD and GtobalInpaa '
lF.}
W
SYNTHETIC DYE DECOLORIZATION USING IMMOBILIZED WHITE-ROT
FLINGI TR4,UET',S I/ERSICOLOR TN CORN COB AND SUCARCANE BAGASSE
AS SUBSTRATE
8.
9.
-
10.
1I.
Suhardi.................
Suci Ramdania, Cella Rahrna Cariss4 Sri Harjati
EFFECT OF TEMPERATURE ON FUNGI ISOLATION
Kristiningrum, Mia Rusmiyati, Denih, Yoshio Watanabe, Hardaning Pranamuda,
Bambang
I3.
301
Marwoto
302
MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AN
ALKALIPHILC BACTERIA ISOLAIED FROM LOCAI, HOT SPRING
CIMANGGU, WEST JAVA
Maria Ulfah, Is Helianti, Niknik Nurhayati......................
COMPARISON OF EXPRESSION OF CELLULASE CENE FROM BICIZIUS
LICHENTFO\?]UI]S FII CLONED IN ESCHENCHU COUIAND
BACILLUS MEGATENUM
Is Helianti, Lina Mulyawati, Maria Ulfah, Niknik Nurhayati ..........................
POTENCY OF THE BACTERIAL CONSORTIA FORMULAAS STARTER FOR
THE TREATMENT SYSTEM OF RIVER THAT CONTAMINATED LAPINDO
MUDFLOW TO SUPPORT POND WATER IN SIDOARJO
Endang
12.
...
303
304
Suarsini....
305
DETERGENT DEGRADER BACTERIA IMI\4OBILIZATION IN TRICKLE BED
REAKTOR EMPLOYED FOR LINEAR ALKYLBENZENE SULFONATE REMOVAL
Ayu Mutiara Hapsari, Sri Harjati Suhardi..................
AMPLIFICATION,CHARACTERIZATION,AND
306
STRUCTUREPREDICTION
OF PCPEY.IlP GENE OF PENICIZ LIUM CHRYSOGENUM FROM BIOTECH
CENTER BPPT COLLECTION
14.
I5.
Dudi Hardianto, Anna Salhrrida, Hardaning Pranamuda, Bambang Marwoto,
and Sasmito Wulyoadi ..............
PRODUCTION OF BIOETHANOL FROM OLD TRUNK OF PALM OIL USING
HOT COMPRESSED WATER
17
.
18,
307
Suyantc, Kotetsu Matsunaga, Hiroyuki Inoue, Kinya Sakanishi......................
IDENTIFICATION AND ISOLATION MICROALGAE FROM MALANG,
EAST JAVA, AS EARLY STEP TO SEARCH THE RENEWABLE B]OLOGICAL
RESOURCES
Prabaningtyas,
16.
...
308
Sitolesrni
309
ISOLATION AND IDENTIFICATION OF INULINASE PRODUCING BACTERIA
FROM HOT SPRINCS IN SOLOK WEST SUMATRA
MindaAzhar, Sumaryati Syukur, Dessy Natalia, Jamsari, Vovien........................................ 310
ISOLATION OF RARE ACTINOMYCETES WITH HIGH TEMPERATURE DRY
HEATING METHOD FROM INDONESIAN SOIL
Fuji NurulAnami, Yudhie Istianto, Siti Handasah, Yoshio Watanabe, Hardaning Pranamuda
and Bambang Marwo1o......................
3l I
SCREENING OF CELULOLYTIC MICROBE FROM INDONESIA'S MARINE
ENVIRONMENT
-
19.
Ifah Munifah, Yusro Nuri Fawzya and Ekowati Chasanah
CITOTOXICITY ASSAY OF ENDOPHYTIC FUNGI ISOLAIED FROM
MEDICINAL PLANT IN EAST LOMBOK BY BRINE SHRIMP LETHALITY TEST
Erwahyuni Endang Prabandari, Tun Tedja Irawadil, Wahono Sumaryono,
Khaswar
312
Syamsu
313
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ISOLATION AND IDENTIFICAIION OF LACTIC ACID BACTERIA ISOLATED
FROM BALI CATTI,E
Ni Putu Desy Aryantini, Ni Wayan Nursini, Komang Ayu Nocianitri,yan Ramona,
21.
22.
23.
24.
25.
Yuji Oda, Tadasu Urashima, Redi Aryanta, I Nengah Sujaya
EFFECT OF AERATION TO THE GROWTH OF DEFINED FUNGI.CONSORTIA
AT LOW PH
314
Farah, Jamhuri, Ahrnad Fauzi, Diana l.{urani and Koesnandar
315
ISOLATION AND IDENTIFICATION OF CHITINOLYTIC BACTERIA
FROM SEA WATER IN WEST SUMATERAFOR FEED PROCESSING
Maria E. Mahata, tresnawati Purwadaria. and Marlina
IMMOBILIZED LACTOBACILLUS ACIDOPHILUS FNCCII' AND BACILLUS
LICHENIFOLUTS FI I.4 FOR DEMINERALIZATION AND DEPROTEINATION
OF CHITIN FROM SHRIMP WASTE
Ofa Suzanti Betha, Siswa Setyahadi, Herman Suyadi
RESPONSE OF OIL PALM (ELAEIS GUIINENSIS JACQ) SEEDLINGS
INOCULATED WITH ARBUSCULAR MYCORRFIIZAL FLINGI AND
MYCORRHIZAL ENDOSYMBIOTIC BACTERIA BICIZ LUS SUBTILIS 2T06
TOWARDS BIOTIC STRESS OF FUNGAL PATHO3EN GANODERT1A
BOI,trINENSE PAT
Yenni Bakhtiar, Sudirman Yahya, Wahono Sumaryono, Meity Suradji Sinaga
3t7
and Sri Wilarso Budi
318
ESTIMATION OF EXPIRY DATE OF BIOPESTICIDE WITH ARTIFICIAL
NEURAL NETWORK MODELING
Diana Dewi, Erwahyuni E. Prabandari, Suyanto, dan Dyah Noor Hidayati
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CHARACTERIZATION OF SAMONELLA TYPHI ISOLAIE,S BY USING NUMERICAL
SYSTEMATIC ANALYSIS OF THEIR SDS.PAGE PROTEIN PROFILES
Charis Amarrntini
Biologl Depa nent, Dlia Wacana Chrislian Uniretsiy
Jalan dr llahidin Sudirohusodo 5-19. yog)oka d.55224.lndonesia.
Phone: +62-274-563929, Fax: + 62-274-5 I 3235, Enail: [email protected]
ABSTRACT
A total of six isolates of Salmonella typhi ficnl the l)pho;d fever patient were characterized by *hole-cell protein profiles using
sodiurl dodecyl sulf'ate-pol1,i,crylamide gel eleclrophoresis (SDS-PAGE). The proliles ofthe protein fingerprintiDg based on the
visualization ofprotein prolile using SDS-P,A.GE wer! convcrted into a binary data thatproduced a phenetic coffelation dendogram.
The binary data were prepared in Progmmmer's File Editor (PFE) software and the data were analyzed using the MVSP PlusVersion 2.0 using the Ss- co€lllcicnt. Clusteriig \!as achicved using the UPGMA algoridrm- A nurnerical classification offie
protcin profilcs showed i\io distinct clusters at 89.7% sinrilarit) level. The firrt cluster comprised iour strairs lvit'h thc similarity
level of96.67,. $ hile the second cluster consisted of t\\,o strains with the similarity value of94.97. As the results of the phcnetic
numericai analysis, delrdogranr has a discrinrinative resolution until strain level and can be used to classify.l 4Pri strains. All
o[the isolatcs formed apparent center ofdiversity rvith the reference strain S. tp], NCTC 786. This finding proved that the six
isolates belonged to rhe species ofS.O,p/ri suBgesting by their relatedness with the refererce strain ofS. o,p,ii NCTC 786
Keyvords: S.;rroliel/a
ipri,
numerical systematic, SDS-PAGE.
INTRODUCTION
Clraractcrization plays an impoftant role as a part olthe miclobial classification. In aitempt to obtain
the knowledge in terms of taxorromic relationship, it is necessary to use a numerical systematic analysis in
the process ol'classifi cation.
A nurnber ofciifferent methods have been used for typing microbial. According to Priest and Austin
( I 993), the component ofchrornosomal DNA, ribosomal RNA, proteins, cell wal1, membrane and Inetabolite
products of the cell can be used for identification and characterization of nricrobial. The protein profile
obtained after electrophoresis has allowed in the identification, classification, and also reclassification of
numerous strains, species and genera ofbacteria (H'fling et al. 1999).
The numerical taxonomic method gave an equal value to every character, thus the closeness oftwo
organisms (called Operational Ta.xonomical Units: OTUs) indicated a resemblance proportion function of
the strains, which were phenetic and not phylogenetic (Logan 1994). The main principle ofthis numerical
taxonomy rvas the use ofa large number ofbiological characten ofan organism (OTU). This large quantity
helped produce a lot of infoimation of the tested OTU in order to get reproducible and full infonnation
classifications (Vatdarnme et al. 1996). This numerical systematic apploach was expectedly able to
ilcrease the ciassification systern significantly and be more effective than what conventional-traditional
classification system could do that treated each stmin unequally or which subjectively chooses a single
character (Logan 1994; Sembiring 2004).
Sqhnonello enterica subsp. enterico serotype Typhi (.!a/zonella enterica serovat Typh|, Salnlonella
typhi) is the agent ofthis typhoid fever. This fever is pathogenic only to human beings and it lemains the
biggest epidenriologic problern in tropical areas, including Indonesia (Parry et a|.2002; Moehario 2009).
Its clinical symptorns are extremely diverse. This condition makes it difncult to estimate the incidence rale
of this fever. There are estiinated l7 to 22 million cases oftyphoid fever worldwide per year and it causes
216,000 to 600,000 deaths annually (Crump e/ a/. 2003; Olsen 2004; Steele 2008).
Sohnonella typhi is belong to Enterobacteriqceae farnily (Holt et al. 1994). As a member of the
family of Enterobacteriaceae, it is important to use the usefulness ofthe technique for screening S. ryP&i
that can differetiated this bacteria from the other member of Enterobacleriaceae fan;Lily (Amaruntini et al.
2009a). Previous lesearch showedthat characterization the S. 4,pri isolates derived fromtheblood culture of
typhoid patients in Southwest Sumba District EastNusa Tenggara based on phenotypic characteristic using
API 20E and API50CHE diagnostics were extremely diverse (Amarantini e/ a/. 2009b). In microbiological
analysis associated with the epidemiological outbreaks, it is crucial to have amore delailed characterization
Bali - Indonesia | 22d - 24'h June 2011 183
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me
I
and an accurate identification (Aqik e, a/. 2005). Msualization ofprotein bands on SDS-PAGE can be used
tbr building a data bank for identification (Logan 1994). Diversity and the amount ofprotein produced in
sorne bacteria are estlrrrated to reach 2000 and it u,as believed to provide sufficient data for developing
identification and typing based on comparative analysis of protein and polypeptide profiles (Towner and
Cockayne 1995).
Based on the given rationale ofthe available literature, it was indispensable to have accurate data
and a strong discriminative ability to diflerentiate the strains ofthe pathogenic bacteria in epidemiological
study. Therelore this study was done in order to characterize the isolates belonging to the,S.,7pri species
derived from the blood culture oftyphoid patients in Souihwest Sumba District East Nusa Tenggara using
combination of SDS-PAGE ofwhole-cell protein with nuinerical systematic analysis.
MATERIALS AND METTIOD
Bacterial strains and grorvth conditions
Six isolates of.9, ryp,lrj used in this research isolated from the blood culture ofthe typhoid patients from
the Karitas Hospital, a private clinic Elopada in East Wewewa Subdistrict in Southwest Sumba District, and
LenCe Moripa Flospital in Waikabubak, West Surnba District. Specimen collections and microbiological
identification metlrods u ele described in thejournal article published previously (Amaraltiri et al.20094
2009b). AII cultures rvere grown at 30"C for 2.1 h on Brain Heart Infusion (BHI) agar before used.
Preparation of soluble rvhole-cell proteins
For each culture, a loopful of ovemiglrt groun on BHI agar was cultivated 100 ml BHI broth and
incubated on a shaking incubator (150 rpm) for l8 h. Cells rvere harvested by centrifugation (3000 g/20
rnin) and washed thtee tirnes with 0.1 M cold Phosphate Buffer Saline (PBS) solution pH 7,4. The pellets
were dissolved in 1.5 rnl PBS and then it was disrupted with sonicator for 30 seconds,6 cycles, and
keeping the amplitude on 0,7. These preparation were transferred to microfuge tubes and centrifuged at
13.000 rpm, 20 rnin at 4 'C. The supernatants rvere collected and incutrated at -20'C befote used.
SDS-PAGE
Profile of soluble whole-cell protein was visualized by using SDS-PAGE (Bollag.and Edelstein
l99l ). The SDS-PAGE was canied out using l0% (w/v) linear slab gelf,Acry-brsacry (30:0,8) 3,333 ml; Tris
HCI lM (pH 8,8) 3,75 mi; 1096 SDS 100 pl; TEMED 10 Al; d(H2O) steril2,707 ml) and 5olo stacking gel
lAcry-bisacty (30:0,8) 0,83i ml; Tris I{Cl I N't (pH 6,6) 0,625 ml; l0% SDS 5 pl; d(H2O) steril 3,437 mll.
The protein concentmtion was determined by Bradford's methods and adjusted to 30 gg/ml. Equal vclume
ofsupematant and loading buffer (5 mM Tris, 2.5olo 2-mercaptoethanol, 1.5% SDS,0.025% bromophenol
blue) were combined and heated in a boiling water bath lor 2 minutes. Subsequently, the samples were
electrophoresed for 3 h at 80 mV using small electrophoresis chaurber (Bio-Rad, USA). In each run a wide
range molecular weight marker (Fermentas SM066l) and the reference strain.S. 4pii NCTC 786 were
included. The gels rvere stained in 0.1% Coomassie Brilliant Blue R250 (Sigma) in methanol:acetic acid:
distilled water (6:2:12) lor overnight with gentle shaking. After that, the gels were destained in methanol:
acetic acid:distilled water (5: t :4) with gentle shaking until the bands became visible.
Numerical systematic analysis ofthe protein profiles
Each protein band visualized on the gel were was analyzed quantitatively by using clustering method.
The matches and mismatches among the bands originated from presence and absence of such bands were
converted into a negative or positive value and it was presented in form of an n x t. The binary data were
prepared in Programmer's File Editor (PFE) software. The data were analyzed using the Multi-Variate
Statistical Package (MVSP) Plus-Version 2.0 (Kovach 1990) using the Sr" (Srz?le Matching Coeficient)
coefficient. Clustering rvas achieved using the UPGMA algorithm (Sneath and Sokal 1973) and tlle result
was presented as dendogram (Sembiring2002).
84 | Bali - Indonesia
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RESULTS
Figure I sholved the soluble whole-cell protein profiles of ,S. 6/p,lri isolates obtained by SDS-PACE.
These isolates were RSK 5.I-MC, RSK 32.1-CCA, RSL 2.1-CCA, BPE 88.1-CCA, BPE 120.1-MC,
RSL 3.1-SSA. To facilitate quantiflcatior of visualization of whole-cell protein bands on SDS-PAGE,
representative diagram illustrating the pattem ofsoluble whole-cell protein bands was made (Fig. 2). Result
ofgrouping rvhole-cell protein profiles was analyzed using S* coefficieut and the UPGMA algorithm. It
was presented in dendogram at Figure 3.
Figure l. Visualization ofwhole-cell protein bands on SDS-PAGE. (A). RSK 5.1-MC, (B). RSK
32.1-CCA, (C). RSL 2.1-CCA, (J). BPE 120.1-MC, (G). BPE 88.1-CCA, (Q). RSL 3.1-SSA and (R).
Reference strain: J. iyphiNCTC 786.
kD,
20ll
70 kD. 6{ koa 50l(oa r
120
IOO
85
kDa
koa
koa
30
kl,a
25
koa
20
-
k|r -
l:-
::-
::-
=
======
-
=
=
ABC
R
Figure 2. The representative diagram illustrating the pattem ofwhole-cell protein bands (A). RSK 5.1MC, (B). RSK 32.1-CCA, (C). RSL 2.1-CCA, (J). BPE 120.1-MC, (C). BPE 88.1-CCA, (Q). RSL 3.1SSA and (R). Reference strain: S. typhiNCTC786.
Bqli - Indonesia | 22'd -
24'h June
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Similarity (%)
Figure 3. Dendogram ofthe six isolates ofS. rypli from Southwest Sumba District, East Nusa Tenggara
and the reference strain (,S. fpfii NCTC 786) based on the analysis cf protein fingerprints using Sr"
coefficient and UPGMA algoritlm.
RSK
5,: SSA
RSK 32.1 CCA
RSL 2,1 CCA
BPE
1t0.1 r"tc
BPEEg.I CCA
RSL 3.1 $SA
S.
tjrriI{CTC 780
Similarity (%)
Figure 4. The dendogram that indicated phenetic relationships among ofthe six isolates of S. typhi from
Southwest Sumba District, East Nusa Tenggara and the reference strain (S. Drpii NCTC 786) based on
phenotypic characterization using API 20E and API 50CHE diagnostics (Arnarantini 2010).
The electrophoretic whole-cell protein pattern of S. typhi isolates (Fig. 1) were quite sirnilar with
some variability, particularly within a range molecular weight between 50,000Da and 120,000Da (Fig. 2).
Two isolates (BPE 88.1 CCA and RSL 3.1 SSA) showed a different pattern with four other isolates (RSK
5.1-MC; RSK 32.|-CCA; RSL 2.1-CCA; BPE 120.1-MC). However, the reference strain (S.4?ri NCTC
786) appeared to have the most distinct protein prcfiles compared with the six isolates tested.
The application of UPGMA ctustering method showed two distinct clusters with 0.82t'% 5 S.", 5
I .0roo%. The first cluster divided into two subclustet s. It contained tbur isolates (RSK 5.1-MC; RSK 32.1 '
CCA; RSL 2.1-CCA; BPE 120.1-MC) in the first subcluster and two isolates (BPE 88.1 CCA and RSL 3.1
SSA) in the second subcluster. The reference strain (S typhi NCTC
86 I Bali - Indonesia | 22d - 24'h June 20ll
7
86) alone fonned the second cluster.
Ptogam
The 4k tnternarional Seminar of Indonesia societr lor Microbiotog) dhd I(IMSJSM outreach
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'lndonesian llrcrobial Resour.es: Dircrsib) and trlobat
and the referel"::11n
The first cluster were well separated from the second cluster. The S.4,Pri isolates
group
with S typhi NCTO
formed a clearly distinct group in S. ryrr,hl strains. They were merged into one
786 on the similarity value of 82. t% (Fig. 3).
DISCUSSION
systematic
In the present study six isolates S. typhi werc analyzed using SDS-PAGE and numedcal
isolates-of'S'
the
six
that
(Fig'
3)
showed
analysis. The dendogram based on protein ingerprinting analysis
l!.'oo*'
typii belonged to the group of S. 4,p&, NCTCIS6 in their similatity index value of 0.8232% 5 S* S
These
of
82.1olo.
value
i-h"r" isoluies merged into on" g-rp with S flpfti NCTC ?86 on the si,rilarity
Johnson
and
Sreath
(0 8) ploposed by
values rvere in agrei,rent t ith the r',ini,r,,r, vaiue ofclassiftcation
that classify these isolates ilrto the 'l
research
previous
with
in
line
was
this
study
of
(1972). The resu-lt
s1'stems (Amarantini et al' 2009a' 2009b; Amarantini
4,7rh species based on API 20E and API 50CFIE
phylogenetic analysis that proved these isolates
fionithe
obtained
2010). The similar fi'ding was also
with the type strain S D'P'i ATCC 19430r
their
relatedness
by
belong to the species ofs. ,)pri suggesting
u'ere identified as a ntember of S. tvphi
the
isolates
all
of
(a.oiuntini ei al. 20,,1). ftiut, it lioveO 1tlat
species.
previous researches sho\ved that SDS-PACE combined with a systematic numerical analysis was
comparative analysis of
able to provide sufficieut data to develop a typing and identificatior based on
al'
2005; Aksakal2010)'
protein profiles (Hd fling et al.l999; Berber2004; Rodrigues et a'l 2004: Agik et
i[
electrophoresis can
These results shou,ed that taxononic resolution obtained using protein fingerprinting
ploved that plotein ptofile
be used for subtl..pir,g u,ithin a single species ofbacteria. Sone resear-cher also
of Proteus
using SDS-pACi nethod can alio used to diflerentiate strains of.Bacil/as species, strains
1987)'
Yousten
and
Cokrnus
1993;
e/
41.
costas
(Berber
2004;
sp
haericus
,pe"i-"r, and strains of.Drrciltas
a discrimination resolulion
Tlre given fact showed that protein proflles obtained fi-om SDS-PAGE provide
strains'
typhi
the
untillhe strain level and therefore can be used lor classifying
'S
(Fig' 3l
The dendogram rvhich ate constructed based on protein fingerprinting analysis
Bd'
using
API 20E and
characterization
typological resemilrnce with the dendogram derived from phenotypic
ei,f sfoHg diagnostics, even though th-ere were differences in the value of similarity (Amarantini 2010)'
two clusten'-The
As it can be observed in Figure 4, t-he phenetic nurnerical analysis clearly also showed
120'l MC)which
first cluster consisted ofsix isolates (RSf S.t SSa; nSf:2.1 CCA; RSL 2.1 CCA; BPE
protein fingerprinting anall'sis. The
was also a member ofthe first cluster in the dendogmm obtained from
All of the isolates form a new
second cluster consisted of only s o,pri NCTC 786 as a referencc strain.
and it had a similar typology
center ofdiversity within the S. 47:iispecies on the similarity value of94.5%
cluster composition based
fiISt
to
the
With
regard
p.ofile.
rvitl.r dendogram which is formJ using protein
"fi,gerprinti,g
RSL 2.1 CCA) had the
CCA
a.d
(RSK
32-l
which
analysis, twi lsolates of
o,., frot.in
legion that was frort
same
the
f;om
sarne similarity value that was egual to 100%. Both isolates were
thal the analysis
indicated
data
Kodi Sub-distiict in Southwest Sumba District East Nusa Tenggara. This
an effective
provide
analysis
of electrophoretic profiles of protein associated with numeiical systetnatic
approach to investigate oftaxonomic relatinships within S typhi strains'
for the studies
This researclihas produced scientific i^foitnation which enriches the basic knowledge
numerical systematic
biosystematics of s. typhi. with the finding oftltis lesearch, it u,as coucluded that
,rpri strains. This
ofS.
assesment
for
taxonomic
analysis of SDS-pACi ofwhole cell proteins is useful
the
strains. It also
distinguish
to
ability
methods produced an accurate data and a strong discriminative
study.
in
epidemiological
needed
could improve a lot of inforrration about the taxonomic lelationship
ACKNOWLEDGMENT
I would like to thank my supervisors Prof. Widya Asmara,
Ph
D', dr' Haripumomo Kuhadiwijaya'
MPH.,Dr.PH,andDrs.LangkahSembiring,MSc.,Ph.D.atGajahMadaUnivelsityJogiakarta.Aspecial
Moripa Hospital
gratitude goes to Karitas Ho;pital in weetJula in Southwest sumba Regency and Lende
the samples'
in waikabubak in west sumba Regency East Nusa Tenggara for their assistance in collecting
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I should also thank Rev. Sr. Sili BoukaADM-the Director of Kadtas Hospital, dr. Loeta Lapoe Moekoethe Director of Lende Moripa Hospital and all doctors of Karitas Hospital for their assisstance for this
research. This research was funded by United Board for Christian Higher Education in Asia.
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ffi
W
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