Aurogene Blue X-Ray Films Biotium – GelRed - Thermo

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Aurogene Cell Colture Media
Biotium – GelRed
Alternativa non tossica all’Etidio Bromuro
Aurogene Agarose Low EEO
Aurogene Blue X-Ray Films
Cod.
ServaGel
20-188
2900
2145
3922203
3922701
3910201
3975903
3956301
4252601
3979502
1537502
06.1938
Descrizione
Preparazione Sample
RIPA Lysis Buffer, 10X 100ml Millipore
Nuclear Extraction Kit 100 assays Millipore
Compartment Protein Extraction Kit Millipore
Bradford reagent, 5x Quant.500ML
BCA Assay Kit Quant.1KIT (480 tests)
Protease Inhibitor Mix M Quant.1VIAL 100X (for 100ml extract)
Dithiothreitol Quant.25G
Mercaptoethanol Quant.50ML
Laemmli Sample Buffer (2x), for SDS PAGE Quant.20ML
Triton(R) X-100 Quant.250ML
Bromophenol Blue-Na-salt Quant.25G
CUVETTE MONOUSO CONF. 100 PZ. cc. 1,5
1068701
1068801
1068702
1068802
1337505
2076703
3593002
3718605
2339003
Preparazione SDS GEL
Acrylamide/Bis Solution, 29:1 Quant.500ML
Acrylamide/Bis Solution, 37.5:1 Quant.500ML
Acrylamide/Bis Solution, 29:1 Quant.4x500ML (30%)
Acrylamide/Bis Solution, 37.5:1 Quant.4x500ML (30%)
Ammonium persulfate Quant.250G
SDS Solution, 20 % Quant.1L
N,N,N',N'-Tetramethyl-ethylenediamine Quant.25ML
Tris(hydroxymethyl)aminomethane Quant.2,5KG
Glycine Quant.5KG
4252901
4253001
BIO-33066
2983403
2983603
3978102
11327----41BL
FT-2-519-580600N
Corsa Elettroforetica/Protein Transfer
SERVA Tris-Glycine/SDS electrophoresis buffer (10x) Quant.1L
SERVA Tris-Glycine native electrophoresis buffer (10x) Quant.1L
HyperPAGE Prestained Protein Marker 500µl
Morpholinoethane sulfonic acid Quant.1KG (MES)
Morpholinopropane sulfonic acid Quant.500G (MOPS)
Sodium chloride Quant.1KG
Rotolo Nitrocellulosa Por. 0.2um 300mmx3m
3MM Blotting Paper 100fogli 580x600mm
1752501
4253101
3507601
3507701
1231001
3342701
3511001
1109303
Gel/Membrane Staining
Coomassie(R) Brilliant Blue R 250 Quant.25G
SERVA Blue R staining (2x) Kit Quant.1KIT
SERVA Silver Staining Kit SDS PAGE Quant.1KIT
SERVA Silver Staining Kit Native PAGE Quant.1KIT
Amido Black 10 B Quant.25G
Ponceau S solution for electrophoresis (0.2 %) Quant.500ML
Silver nitrate Quant.25G
Ethanol undenaturated absolute Quant.2,5L
SC-2325
75892 1 LT
75892 5 LT
77500 1 LT
77500 5 LT
3979601
Immunoblotting
Blotto, non-fat dry milk 500g (SantaCruz)
TBS, 20X SOLUTION
TBS, 20X SOLUTION
TBS WITH TWEEN -20 (20X)
TBS WITH TWEEN -20 (20X)
Tween(R) 20 Quant.100ML
AU1101
AU1102
XLS10,0500
P7042-5GA
P7167-5GA
ECL - Lastre - Sviluppo
Blue X Ray film 13 X 18 cm - 100 lastre
Blue X Ray film 18 X 24 cm - 100 lastre
WESTAR® Nova 2011 HRP Detection Substrate 500ml Kit
Kodak® autoradiography films GBX developer/replenisher 5 Gallons
Kodak® autoradiography films GBX fixer/replenisher 5 Gallons
Acrylamide Solution
HyperPAGE Marker
Novità Aurogene:
Higher sensitivity – use less antibody, load less
sample, carry out shorter exposures
Long-lasting signal – never rush to image your
blot again
Convenience – just point, spray, and expose to
film
WesternBright MCF fluorescent Western blotting kit allows the
assay of two proteins at once, increasing the quality and quantity of
information that can be gained from a single blot. Assay a loading
control alongside a protein of interest. Assay phosphorylated and
non-phosphorylated isoforms of a protein simultaneously. The
WesternBright protocol saves time and money since there is no
need to strip and re-probe a blot, no use of disposable film, and the
blot can be imaged immediately, without drying.
The fluorescent dyes provided with WesternBright MCF outperform
Cy dyes and allow detection of low pg amounts of protein.
WesternBright ECL detects equal amounts of protein
with up to ten times less antibody. Duplicate slot blots
containing serial dilutions of transferrin were probed
with the antibody dilutions shown, and detected with
either WesternBright ECL or Pierce ECL (Thermo
Scientific) according to the manufacturers’ instructions.
The blots were imaged simultaneously on the same piece
of film. WesternBright ECL produced the same signal
with ten times less primary and five times less secondary
antibody.
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Calsystem Srl
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Phone: +39 0984 461203
Organigramma Societario:
Contatti:
Dr. Antonio Petrone, PhD – Direttore Generale
Informazioni: [email protected]
Amministrazione: [email protected]
Assistenza Tecnica: [email protected]
Logistica: [email protected]
Commerciale: [email protected]
Mail: [email protected]
Mobile: +39 328 7195178
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Mail: [email protected]
Mobile: +39 328 7195178
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Phone +39 06 98185510
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Fax : +39 06 89280412
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