accessories handbook
Transcription
accessories handbook
Unlimited possibilities Accessories Handbook for NanoWizard, ForceRobot and CellHesion systems ® ® ® valid from 2015 Unmatched flexibility & modularity More than 100 accessories More than 30 operating modes the NanoWizard , ForceRobot ® ® and CellHesion family ® CellHesion®® 200 Optional heads NanoWizard®® ULTRA Speed A for fast-scanning and super-resolution AFM ForceRobot®® 300 NanoWizard ® ® NanoScience and BioScience versions Heads Vortis™ A Combi controller Control electronics options Software Vortis A SPMControl Can also drive the JPK NanoTracker™ Experiment Control™ Experiment Planner™ System options QI™ Advanced 3-axes Manipulation & lithography Advanced Force Spectroscopy Advanced AC modes CellHesion® Module TAO™ Module BioMAT™ Motorized precision stage SPM Control & DATA Processing Direct Overlay ™ PetriDishHeater™ for BioMAT™ Stages Vortis™ A Advanced SPMControl ™ ™ TopView Optics™ 2-axes Life sciences stage HCS™ Standard stage HybridStage™ Heating cooling stage ECCell™ – Electrochemisty cell Temperature control Fluid cells & cantilever holders Electrical modes & application modules Extras & OEM PetriDishHeater™ SmallCell™ ECCell™ BioCell™ Small volume SmallCell™ Electrochemistry cell Standard Cantilever Holder HTHS™ HCM™ Heating cooling module Supercut Cantilever Holder Side-view Cantilever Holder High temperature heating stage Cantilever Holder with electrical tip connection PetriDish Holder High Voltage Sample Bias Amplifier Conductive AFM module Kelvin Probe module HyperDrive™ fluid imaging package CoverslipHolder with electrical sample connection Fast scanning package 8x FluidicsModule™ Acoustic enclosure Vibration isolation Micromanipulator Fiber coupled detection module Cameras Setups and accessories for BioScience applications highlighted with LIGHT BLUE BOXES Setups and accessories for NanoScience applications highlighted with GRAY BOXES Typical BioScience applications and research fields: Typical NanoScience applications and research fields: High resolution imaging in air and liquid of single molecules such as proteins, DNA/ RNA, carbohydrates, lipids, Live Cell Imaging, bacteria and virus characterization, cellular adhesion and cytomechanics, molecular interactions, receptor-ligand, antibody-antigen, molecular unfolding, molecular recognition, biomaterials, compound and implant characterization, material properties such as adhesion, elasticity, hardness; drug formulation, encapsulation and aging studies and many more High resolution imaging in air and liquid of single molecules, thin films, nanoparticles or nanowires, surface properties characterization such as adhesion, stiffness, friction, conductivity, charges, hardness; polymer science, single molecule force measurements, smart organic materials, surface properties of conjugated polymers, biosensors, degradable materials, electrochemistry, colloidal probe, surface forces and interface phenomena; and many more For more detailed information about all options see the brochures and product notes or visit www.jpk.com. 2 CoverslipHolder accessories handbook Contents 1 2 3 4 Control electronics options Vortis™ A SPMControl Station Vortis™ A Advanced SPMControl Station Vortis™ A Combi controller 4 7 System options 5 NanoWizard® 4a for high resolution and fast scanning NanoWizard® ULTRA Speed A for fast-scanning and super-resolution AFM ForceRobot® 300 CellHesion® 200 TAO™ Tip Assisted Optics module BioMaterials Workstation BioMAT™ CellHesion® module Software modules ExperimentControll™ ExperimentPlanner™ software module QI™ Advanced software module Advanced AC modes module DirectOverlay™ module NanoLithography/NanoManipulation module MicroRheology module Advanced Force Spectroscopy module 7 8 9 Applications modules and modes 8 Fast scanning package HyperDrive™ fluid imaging package OEM micropipettes Electrical sample connection module CoverslipHolder with electrical sample connection Kelvin Probe (KPM) and Scanning Capacitance (SCM) Microscopy module Conductive AFM module Conductive AFM module – Enclosed Volume Tunneling Current Conductive AFM (TC-CAFM) module High Voltage Sample Bias Amplifier JPK 8x FluidicsModule™ ForceWheel™ 5 Sample stages HybridStage™ Motorized precision stage Manual precision stage Sample holder for large samples Standard stage 11 6 Cantilever holder options Fixed-spring cantilever holder Standard cantilever holder Supercut version cantilever holder Cantilever holder with electrical tip connection Side-view cantilever holder 12 Environmental control options – fluid cells and temperature controls BioCell™ CoverslipHolder PetriDishHeater™ PetriDishHeater™ for BioMAT™ PetriDishHolder™ OEM Humidifier from LIS SmallCell™ for harsh environments 3-port small volume SmallCell™ High Temperature Heating Stage (HTHS™) Heating Cooling Module (HCM™) Heating Cooling Stage (HCS™) Cryostage Electrochemistry solutions Temperature controlled ECCell™ Heating Cooling Stage (HCS™) with electrochemistry cell OEM Potentiostats from IPS Vibration and acoustic isolation Active vibration isolation from Accurion Active vibration isolation from Table Stable Active air damping tables from TMC JPK acoustic enclosure JPK base for acoustic enclosure 14 17 18 10 Optics accessories 19 TopViewOptics™ TopViewOptics™ with boom stand TopViewOptics™ for use with inverted optical microscopes or the JPK BioMAT™ Raman reflector kit Upright Fluorescence Microscope (UFM) Kit Fiber coupled detection module Inverted optical microscope configurations 11 OEM solutions and small parts Stand for AFM head JPK sample holder for small samples JPK cable anchorage pillow JPK cantilever changing tool JPK biocompatible glue JPK spare parts OEM syringe pumps CCD, EMCCD and sCMOS cameras OEM indentation solutions OEM materials testing modules for mechanical properties OEM incubators for Live Cell experiments under controlled environments OEM glove box systems for NanoWizard® AFMs 20 3 1 Control electronics options Vortis™ A SPMControl Station State-of-the-art digital controller with lowest noise levels. Key features High speed 16bit AD conversion with 60 MHz for the photodetector signals 24 bit ultra precise ADC with 2.5 MHz High speed lock-in amplifier technology for precise amplitude and phase detection High speed data capture with optional burst mode Modular hybrid analog/digital design with latest PPC technology (PowerPC @ 660 MHz) Gigabit Ethernet interface for fast data link Number of data points that can be captured continuously: restricted only by HDD Thermal noise acquisition up to 3.25 MHz Vortis™ A Advanced SPMControl Station The most advanced SPM controller. (see product note) Key features State-of-the-art digital controller with lowest noise levels and highest number of signal channels 4 high speed 16 bit ADC channels with 60 MHz 12 ADCs with 18 bit with 800 kHz 24 bit ultra precise ADC with 2.5 MHz 3 channels 14 bit DAC with 120 MHz 8 channels 24 bit DAC with 400 kHz 2 high-speed and 1 mid-speed lock-in amplifiers for precise amplitude and phase detection High speed data capture Modular hybrid analog/digital design with latest PPC technology (PowerPC @ 660 MHz) Gigabit Ethernet interface for fast data link Number of data points that can be captured continuously: restricted only by HDD Thermal noise acquisition up to 3.25 MHz 2 gated photon counters Vortis™ A Combi controller The combined high-end solution for JPK Scanning Probe Microscopes and Optical Tweezers systems. (see product note) Key features One system for all nano-force applications Simple conversion from SPM to OT control 4 high speed 16 bit ADC channels with 60 MHz 12 ADCs with 18 bit and 800 kHz 3 ADCs with 18 bit and 100 kHz (for scanning) 24 bit ultra precise ADC with 2.5 MHz 3 high speed channels 14 bit DAC and 120 MHz 8 channels 24 bit DAC with 400 kHz 2 channels 16 bit DAC and 1.6 MHz 1 × 14 bit DDS with 240 MHz +/- 1 V @ 50 Ω 3 × 16 bit reference output up to 15 MHz 2 high speed lock-in amplifiers running with 60 MHz, frequency range from 1kHz to 10MHz CellHesion® 4 1 mid speed lock-in amplifiers with 800 kHz, frequency range up to 300 kHz Multiple feedback loops are implemented with fully configurable transfer function Phase-locked-loop (PLL) integrated for Frequency Modulation Thermal noise acquisition up to 3.25 MHz Software controlled digital filters High speed data capture 3 channels closed-loop control via capacitive sensors 3 channels -20 to +120 V scanner drive (4. channel optional) NanoWizard® ForceRobot® NanoTracker™ 2 System options NanoWizard® 4a for high resolution and fast scanning The new NanoWizard® 4a AFM head combines atomic resolution and fast-scanning possibility with line rates up to 70Hz in a system with a large scan range of 100 µm. (see NanoWizard® 4a brochure) Key features Tip-scanner technology for safe and easy operation in air, gases, and fluids Full transmission optical capabilities with standard condenser; e.g. bright field, phase contrast, Hoffman modulation or DIC simultaneous to AFM imaging with inverted research microscopes Advanced optical techniques simultaneous to AFM like FRET, TIRF, FLIM, FCS, single molecule detection, epi-fluorescence or confocal laser scanning (CLSM) with optional optical microscopes Simultaneous operation with optical super resolution techniques like STED, STORM/PALM and SIM JPK´s DirectOverlay™ feature for perfect combination of AFM and optical microscopy Open hard and software architecture Optimum performance by active vibration isolation table Comprehensive set of AFM modes like JPKs easy-to-use QI™ mode, contact mode with lateral force, advanced AC modes like non-contact, phase detection, electrical and magnetic imaging modes NanoWizard® ULTRA Speed A for fast-scanning and super-resolution AFM The NanoWizard® ULTRA Speed A AFM head combines true atomic resolution and fast scanning with line rates up to 300Hz. (see NanoWizard® ULTRA Speed A brochure) Key features Fast-scanning up to 300Hz line rate for tracking dynamic processes Comes with the easy-to-use QI™ mode for quantitative material property mapping Atomic resolution in closed-loop mode by lowest scanner, position-sensor and detection-system noise level Unique integration with optical microscopy by tip-scanning design and DirectOverlay™ feature for most precise correlative microscopy Fully flexible design with the widest range of modes and accessories ForceRobot® 300 The innovative force spectroscope with fully automated work flow. Take more than 80,000 force curves within 24 h while varying parameters such as temperature or loading rate. (see ForceRobot® 300 brochure) Key features Fully automated force spectroscope with highest flexibility Cutting edge force spectroscopy and force mapping in combination with single molecule fluorescence Automated laser and detector alignment CellHesion® NanoWizard® ForceRobot® Automated cantilever drift compensation Advanced and dedicated software with ExperimentPlanner™, RampDesigner™ and built-in data batch processing Fits to the Vortis™ A controller NanoTracker™ 5 CellHesion® 200 The single cell force testing solution for cell adhesion and elasticity studies. Specialized platform for cell adhesion and cell mechanics measurements from single molecules to entire cells. (see CellHesion® 200 brochure) Key features Cantilever sensor lifting system with >110 µm travel range with closed-loop control through high-speed capacitive sensor feedback Integrates with advanced optical imaging (DIC, CLSM, TIRF, FRET...) Fits to the Vortis™ A controller TAO™ Tip Assisted Optics module Specialized sample stage for advanced experiments combining AFM and optical spectroscopy based on proven NanoWizard® technology. 2 axis and 3 axis version available. (see TAO™ module product note) Key features Ultimate platform for TERS, scatter type SNOM, single molecule imaging/spectroscopy experiments NanoOptical studies such as quenching, field enhancement or bleaching Integrated sample scanning confocal imaging Integrates with conventional and advanced optical imaging (DIC, Phase contrast, CLSM, TIRF, FRET...) Flexible software system solution for experimental freedom and remote operation Ultimate flexibility through simultaneous control of up to 6 scan axes with closed loop precision (linearized with capacitive sensors): AFM tip scanner: 100 × 100 × 15 µm3 Sample scanner: 100 × 100 × 10 µm3 100 × 100 µm2 30 × 30 × 10 µm3 Fits to the Vortis™ A Advanced controller BioMaterials Workstation BioMAT™ Completely integrated system comprising upright optical microscope and AFM. The specialist for investigations of opaque samples in life and material sciences. (see BioMAT™ brochure) Key features Studies on non-transparent substrates or samples with AFM and optics Outstanding reproducibility of the focussed position (ROI) with both systems Unique capability to investigate the ROI precisely with optics and AFM Also perfect during operation in liquids Wide range of applications: Biochips, cell chips or patterned substrates for cell adhesion, plant cell applications, microbiology, tissue engineering, ... Flexible concept with shuttle stage Both techniques can be operated even in different laboratory rooms CellHesion® module As an add-on to the NanoWizard®, the CellHesion® module combines the capabilities of the BioAFM with precise adhesion force measurements and all optical microscopy features simultaneously. (see CellHesion® module product note) Key features Simultaneous focus tracking optional Integrates with advanced optical imaging 100 µm additional z-travel Perfect hardware linearization through (DIC, Confocal microscopy, FRET...) capacitive sensors CellHesion® 6 NanoWizard® ForceRobot® NanoTracker™ 3 Software modules ExperimentControl™ Remote control and monitoring of complex and long-term experiments Key features Remote control and monitoring of complex and long-term experiments via the internet by a PC, tablet or smartphone Simplifies setting up the instrument especially inside an acoustic hood Continuous status update of the instrument Tablet with holder is included ExperimentPlanner™ software module For comprehensive planning of an experiment with all of the external parameters. Key features Scanners, motors, light sources, detectors, environmental control options etc can be master controlled and timed Delivers full control over all system options Design of advanced experiments QI™ Advanced software module For NanoWizard® systems; delivers quantitative mechanical properties. (see QI™ brochure) Key features Depending on application, it can also deliver electrical conductivity or molecular recognition in a single scan Contact Point Imaging (CPI) mode for extremely soft and inhomogeneous surfaces QI™ Advanced imaging mode for conductive measu rements in combination with the JPK CAFM module Any kind of sample can be imaged: samples with steep edges, loosely attached samples, soft, sticky and brittle samples Works under ambient conditions and in fluid Quick to learn and easy to operate Provides additionally adhesion, stiffness and dissipation data while scanning Advanced AC modes module Software module for NanoWizard® systems Key features Comprehensive range of advanced dynamic feedback modes Phase Modulation (PM), Frequency Modulation (FM) or self-excitation FM CellHesion® NanoWizard® ForceRobot® Modes can be combined with Amplitude Gain Control (AGC) Higher harmonics with extra lock-in amplifier Requires cantilever holder with DirectDrive™ capability NanoTracker™ 7 DirectOverlay™ module Perfect integration of optical and AFM data – JPK‘s proprietary and patented solution for perfect overlay of optical and AFM information. Key features Perfect overlay of optical and AFM data with sub-diffraction limit precision Direct “in optical image” selection of AFM measurements (imaging and force curves) Dramatic reduction of overview image scanning in AFM, giving faster results & lower tip contamination Optical image navigation to specific regions of interest, even without AFM scanning. This protects functionalized tips for molecular recognition, avoiding tip passivation from image scanning before the force measurements. NanoLithography/NanoManipulation module Software module for NanoWizard® systems. Key features 350nm Free-hand or vector-based patterns are possible Moving objects or scratching the substrate with controlled force are possible Pattern creation by electrical current such as anodic oxidation MicroRheology module For visco-elastic properties of living cells and other samples such as gels or foams. Key features Extension to RampDesigner™ offering sine modulation segments Perform oscillating measurements with settings for amplitude and frequency Force history can be defined by the user through choice of preceding and following segments in RampDesigner™ Sine modulation analysis in the Data Processing software fits amplitude and phase of response Advanced Force Spectroscopy module For advanced force measurement experiments from single protein unfolding, DNA stretching to probing of cells and tissue. Key features Tuneable frequency sweep from 0.1 to 10 kHz Setting of user-defined patterns for rheology applications Integrated RampDesigner™ for user defined segmentation of force curves Advanced force clamp 4 Applications modules and modes Fast scanning package Fast scanning option for NanoWizard® 4a Key features > 70 Hz line rate for scan ranges up to 2 µm Fast z-scanner and mechanical adaptation Ultrastable JPK DirectDrive™ cantilever excitation for AC modes CellHesion® 8 >70 kHz z-scanner resonance frequency Software upgrade for fast scanning Supports FM-AFM and self-excitation FM-AFM Fast data acquisition NanoWizard® ForceRobot® NanoTracker™ HyperDrive™ fluid imaging package For highest resolution imaging of small structured samples in liquid. (see jpk website for more information) Key features Optional for Nanowizard® Nanoscience and Bioscience version Minimized tip-sample interaction by using smallest amplitudes Consists of a special cantilever holder with direct drive functionality, and a control software module OEM micropipettes JPK offers a micropipette system, e.g. CellTram® models from Eppendorf, together with NanoWizard® or CellHesion® 200 systems. Electrical sample connection module Key features Accommodates electrical conductive samples such as AFM metal stubs by magnetic fixation or transparent samples such as ITO coated glass coverslips Insert for Life Science stages CoverslipHolder with electrical sample connection For electrical measurements such as Conductive AFM on a coverslip in combination with high NA optics. Key features Accommodates electrical conductive samples and transparent samples such as ITO coated glass coverslips Insert for Life Science stages Kelvin Probe (KPM) and Scanning Capacitance (SCM) Microscopy module Option for nanoscale mapping of surface potential distribution. Key features External bias electronics module (+/-10 V) Software interface Enclosed volume version also available Surface Potential Mapping Electrical tip connection CellHesion® NanoWizard® ForceRobot® NanoTracker™ 9 Conductive AFM module For conductivity and I/V measurements on the nanoscale. Key features High-performance conductive AFM experiments Recording I/V curves Bias voltage range ± 10 V Noise limit 0.55 pA RMS Sample holders for opaque samples Sample holder for transparent samples works with all optical microscopes Conductive AFM module – Enclosed Volume For experiments in inert atmosphere; for conductivity and I/V measurements on the nanoscale under controlled environmental conditions. Key features High-performance conductive AFM experiments under user defined gas atmosphere Recording I/V curves Bias voltage range ± 10 V Noise limit 0.55 pA RMS Sample holders for opaque samples Sample holder for transparent samples works with all optical microscopes Tunneling Current Conductive AFM (TC-CAFM) module For low-conducting samples. Key features Highest-performance, lowest current conductive AFM experiments Records local I/V curves Bias voltage range ± 10 V Current range with a gain 1*109 up to ± 10 nA with a noise limit 100fA RMS in imaging bandwidth Other current ranges on request (see JPK Conductive AFM module product note) Optional version with closed sample volume for experiments in defined atmosphere with a volume of 140 µL In combination with QI™ Advanced mode and NanoWizard® mechanical and electrical data can be correlated on the nanoscale in a single scan With QI™ Advanced high resolution and low noise, current mapping on fragile, brittle and loosely attached samples with virtually no tip wear due to eliminated lateral forces High Voltage Sample Bias Amplifier Can be used for biasing a sample, e.g., in electro-optical experiments or in Piezo-Response Force Microscopy (PFM). Key features +/- 100 V can be applied between sample and tip CellHesion® 10 Software controlled NanoWizard® ForceRobot® NanoTracker™ JPK 8x FluidicsModule™ Key features JPK FluidicsModule™ with up to 8 different liquids such as buffer solutions Software-controlled flow rate Automated incubation or adding of chemicals Comprehensive safety package ForceWheel™ JPK‘s ForceWheel™ handheld accessory for most sensitive experiment control, e.g., for force spectroscopy. Key features Continuous setting of gains and setpoints Easy attachment of cells or beads with cantilever tip Connected via USB Manual force curve acquisition 5 Sample stages HybridStage™ Modular piezo based sample scanner stage combined with motorized coarse XY sample movement. With the HybridStage™, accurate and highly reproducible Z-stacks, important for image reconstruction, can be performed while the AFM stays in place. Key features Combined piezo and motorized stages for automated mapping or scanning of large areas Maps large objects such as cell scaffolds, printed 3D structures, micro-spheres or capsules and micro/ nano structured surfaces over millimeter distances Cell/cell or cell/substrate adhesion in 3D with a large pulling range up to 200 x 200 x 200 µm³ Mechanical properties of plant cells, cell layers, cartilage, parasites, scaffolds, bones or tissue typical with rough surfaces over a mm range Optional XY or XYZ piezo flexure scanners with a large travel range (depending on the application) can be implemented Automated and streamlined workflow with the motorized HybridStage™ Motorized precision stage Offers automatic motion control for precise positioning of the sample relative to optical axis and AFM probe. Key features Transmission illumination capability for inverted optical microscopes like Olympus IX line Zeiss Axio Observer/Axiovert 100/135/200 Nikon TE 2000 or Ti series Leica DMI/DMi lines Please specify model upon order Travel range of the sample 20 × 20 mm² Step size (Resolution): ≤ 1 µm Repeatability (unidirectional): ≤ 2 µm CellHesion® NanoWizard® ForceRobot® Maximum velocity: 1 mm/s With joystick or software control For automated tiling or mapping applications Manual precision positioning of the AFM tip Stand alone use or for inverted optical microscopes Compatible with all JPK add-ons, glass slides, Petri dishes etc. Compatible with NanoWizard®, ForceRobot® 300 and CellHesion® 200 heads NanoTracker™ 11 Manual precision stage Offers sub-micron resolution & fine motion control for precise positioning of the AFM tip relative to optical axis and the sample. Key features Transmission illumination capability for inverted optical microscopes like Olympus IX line Zeiss Axio Observer/Axiovert 100/135/200 Nikon TE 2000 or Ti series Leica DMI/DMi lines Please specify model upon order Travel range of the sample 20 × 20 mm² Precise positioning of the sample holder with thermally decoupled, magnetic fixation Flexible design for fluid cells, temperature control options, and custom sample holders Detachable from optical microscope for stand alone operation Drift compensated design Compatible with NanoWizard®, ForceRobot® 300 and CellHesion® 200 heads Sample holder for large samples The holder module fits directly to the manual or motorized precision stage. Key features The holder can accommodate larger samples such as microchips or wafers and is equipped with spring clips which can be varied Standard stage Offers fine motion control for precise positioning of the AFM tip relative to the sample. Key features Flexible design for fluid cells and different sample holders Rigid, chemical resistive surface Maximum flexibility for customized sample mounting Travel range of the AFM head (tip positioning) 10 × 10 mm² Stand alone use in combination with JPK TopViewOptics™ Magnetic sample holder 6 Cantilever holder options Fixed-spring cantilever holder The standard holder for all-round applications. Key features For air or aqueous solutions Compatible with JPK BioCell™, CoverslipHolder™ or PetriDishHeater ™ for easier navigation Robust design using glass and medical steel CellHesion® 12 Measurements in fluid droplets or in fluid baths like Petri dishes or home made fluid cells Immersible to 8 mm fluid depth Easy to clean Easy handling even with gloves NanoWizard® ForceRobot® NanoTracker™ Standard cantilever holder For all-round applications with a large optical field of view. Key features Chemically inert cantilever holder Optimized for the use with top-view optics Robust design using glass and medical steel or gold spring only Immersible to 6 mm fluid depth Easy to clean by detergents, ultrasonic bath or autoclaving Easy handling, even with gloves Measurements in droplets or in fluid baths like Petri dishes or home made fluid cells Works in air and liquid, including aggressive media Supercut version cantilever holder The holder is designed for use in air or liquids with removable cantilever spring for maximum cleaning results. Key features Chemically inert cantilever holder For use with JPK BioCell™, CoverslipHolder™ or PetriDishHeater ™ for easier navigation Robust design using glass and medical steel or gold spring only Immersible to 6 mm fluid depth Easy to clean by detergents, ultrasonic bath or autoclaving Easy handling, even with gloves Measurements in droplets or in fluid baths like Petri dishes or home made fluid cells Works in air and liquid, including aggressive media Cantilever holder with electrical tip connection The holder is designed for use in air or liquids with a fixed cantilever spring and an electrical tip connection. Key features For air or aqueous solutions Compatible with JPK BioCell™, CoverslipHolder™ or PetriDishHeater ™ for easier navigation Measurements in fluid droplets or in fluid baths like Petri dishes or home made fluid cells Robust design using glass and medical steel Immersible to 8 mm fluid depth Easy to clean Electrical tip connection Side-view cantilever holder This holder is designed for use in combination with inverted optical microscopes to observe the cantilever region from the side. Key features For use with JPK BioCell™, CoverslipHolder™ or PetriDishHeater ™ for easier navigation Measurements in fluid droplets or in fluid baths like Petri dishes or home made fluid cells CellHesion® NanoWizard® ForceRobot® Immersible to 8 mm fluid depth Easy to clean Easy handling, even with gloves NanoTracker™ 13 7 Environmental control options – fluid cells and temperature controls BioCell™ The BioCell™ allows for the temperature-dependent study of living cells or single molecules with the NanoWizard® AFM, CellHesion® 200 and ForceRobot® 300 in their native environment. It is therefore particularly suited for applications in cell biology and biomedical research. As an add-on for inverted optical microscopes the NanoWizard® AFM in combination with the BioCell™ offers the unique possibility to simultaneously study temperature-dependent processes like cell motility and metabolism with the AFM and simultaneous advanced optical imaging. During AFM measurement all the optical microscope‘s operation modes, in particular contrast techniques, remain constantly available. Even high-resolution fluorescence confocal microscopy and single-molecule fluorescence techniques are possible since objective lenses with highest numerical aperture can be used. Key features Connections for liquid perfusion and gas flow (e.g., CO2 mix) Fully compatible with JPK‘s range of NanoWizard® AFM, CellHesion® and ForceRobot® systems Drift-minimized in all direction Insert for Life Science stage Temperature range: 15 to 60°C Resolution: 0.1°C Quick-exchange cover slip bottom CoverslipHolder With the same design as the BioCell™ but without temperature control, the CoverslipHolder is the ultimate high resolution liquid cell with coverslip bottom. Key features Insert for Life Science stage Closed liquid cell with standard coverslip bottom High-N.A. immersion optics possible by exchange able cover slip bottom (0.17 mm thickness) Ports for perfusion & gas flow, e.g., CO2 or nitrogen Superior design for combining AFM and confocal laser scanning Made of glass, steel or gold, and silicon only Easy to clean, also in ultrasonic bath Autoclavable inlet PetriDishHeater™ The heater system is fully compatible with JPK‘s range of NanoWizard® AFM and CellHesion® systems. This means all the standard imaging and measurement techniques may be applied at temperatures up to 60 ˚C with 0.1 ˚C precision. These include fluorescence, brightfield, phase contrast and DIC, AFM imaging and cell mechanics study. The system also offers perfusion control employing standard syringe pumps and gas flow of carbon dioxide possible for long term cell studies. Glass bottomed dishes are important for high numerical aperture fluorescence experiments such as confocal laser scanning used in combination with AFM. Key features Insert for Life Science stage Temperature range: room temperature up to 60 °C Compatible with 35 × 10 mm Petri dishes from WPI and TPP - plastic or plastic with glass bottom Adaptors for other suppliers such as Matek, Willco, IWAKI, Ibidi, and BD upon request CellHesion® 14 Ports for perfusion tubes and gas supply (only available for certain types of Petri dishes) Software control Temperature stability 0.1 K Fully compatible with JPK‘s range of NanoWizard® AFM and CellHesion® systems Fits to all inverted microscope brands in combination with a JPK sample stage NanoWizard® ForceRobot® NanoTracker™ PetriDishHeater™ for BioMAT™ Shuttle stage with integrated Petri dish heater for JPK BioMaterials workstation. Key features Temperature range: room temperature up to 60°C Compatible with 35 × 10 mm Petri dishes from WPI and TPP – plastic or plastic with glass bottom Dipping lenses need to be compatible with type of Petri dish Temperature stability 0.1 K Software control PetriDishHolder™ Designed like the PetriDishHeater™, but without temperature control, the PetriDishHolder™ is the ultimate liquid cell for high resolution experiments in a Petri dish. Key features Insert for Life Science stage Compatible with 35x10mm Petri dishes from WPI and TPP (plastic or plastic with glass bottom) Adaptors for other suppliers such as Matek, Willco, IWAKI, Ibidi, and BC upon request Ports for perfusion tubes and gas supply (only available for certain types of Petri dishes) Openings for immersion medium application Silicone anti-evaporation seal OEM Humidifier from LIS Together with the BioCell™ and the PetriDishHeater™, JPK offers an external humidifier from LIFE IMAGING SERVICE (LIS), consisting of: Key features Digital gas flow sensor (< 3 bar in; < 1 bar out) Tubing (4 × 1 mm, 6 m) Humidity sensor Humidity sensor capsule (from JPK, with 2 screws M 2.5×6 mm) Humidity controller Set of tubings Insulating tubing (OD/ID - 4×2 mm) Transfer tubing to fluid chamber © LIFE IMAGING SERVICES SmallCell™ for harsh environments The perfect solution for smallest volume experiments in a hermetically sealed environment, the SmallCell™ is perfect for fluid or gas exchange. The SmallCell™ is compatible with the JPK temperature control systems like the HTHS™, HCM™ and the HCS™. Key features Solvent, acid, chloroform and acetone resistant Multi-purpose closed fluid cell for small volumes (< 150 µl) Inlet and outlet ports for perfusion experiments CellHesion® NanoWizard® ForceRobot® Glass, steel or gold, and resistive seal only Easy to clean, also in ultrasonic bath Autoclavable Temperature range up to 100 °C NanoTracker™ 15 3-port small volume SmallCell™ The perfect solution for smallest volume experiments in a hermetically sealed environment, the small volume SmallCell™ is perfect for aqueous solutions exchange. The SmallCell™ is compatible with the JPK temperature control systems like the HTHS™, HCM™ and the HCS™. Key features Closed fluid cell for minimized volumes (< 60 µl) Optimized for aqueous solutions Inlet and outlet ports for perfusion experiments with Luer connectors Temperature range up to 80 °C Extra port for small amounts of liquids such as drugs, proteins etc. Polycarbonate, steel or gold, and seal only Easy to clean, also in ultrasonic bath Autoclavable High Temperature Heating Stage (HTHS™) This high performance heating stage is designed for demanding polymer applications as well as for stable long time temperature studies of single molecules or nanoparticles. Key features Temperature range from ambient to 300 °C in air and ambient to 80 °C in fluid when used together with the JPK SmallCell™ Temperature resolution 0.1 °C Drift minimized in all directions for full tempera ture ramps simultaneously with AFM imaging No transmission illumination possible Heating Cooling Module (HCM™) Designed for heating and cooling experiments in gas or liquids with minimized drift in all dimensions. Key features Temperature range from -30 °C to 120 °C ~0-80 °C temperature range for experiments in aqueous solutions Temperature resolution 0.1 °C Compatible with JPK Manual precision stage and Motorized precision stage No transmission illumination possible Heating Cooling Stage (HCS™) Designed for AFM experiments in air or liquids from 0 °C up to 100 °C with minimized drift in all dimensions – HCS™ follows the same design criteria as HTHS™. Key features Temperature range from 0 °C to 100 °C Temperature resolution 0.1 °C Twin stage thermoelectric element Drift minimized in all directions for full temperature ramps simultaneous to AFM imaging CellHesion® 16 Heatpipe design – no water cooling or fan required Can be used together with closed fluid cells such as SmallCell™ NanoWizard® ForceRobot® NanoTracker™ Cryostage JPK offers specialized cryostages together with NanoWizard® systems for cooling applications up to -120°C. Please ask the JPK applications team for more information. 8 Electrochemistry solutions Temperature controlled ECCell™ Electrochemistry with temperature control and transmission optics such as phase contrast, DIC or fluorescence together with AFM. The unique combination of temperature controlled electrochemical measurements with high-end optics makes the ECCell™ the ideal tool for combined studies of temperature and potential sensitive biomolecules. Key features Works with transparent or non-transparent substrates Accommodates standard coverslips, metal or silicon chips or other substrates Temperature range from ambient to 60 °C max. 1350 µl liquid volume with perfusion capability Sealed design for inert gas filling Wire electrodes and miniature reference electrode Suitable for conductive films or substrates (e.g., ITO coated glass) Compatible with common potentiostats Allows the use of high numerical aperture lenses for optimum fluorescence performance Unrestricted high resolution AFM imaging Heating Cooling Stage (HCS™) with electrochemistry cell Designed for electrochemistry AFM experiments with controlled heating and cooling of the sample. Key features For opaque samples Temperature range from 0°C to 100°C Temperature resolution 0.1°C Twin stage thermoelectric element Drift minimized in all directions for full temperature ramps simultaneous to AFM imaging Heatpipe design – no water cooling or fan required Top-view optical access for easy navigation max. 1350 µl liquid volume with perfusion capability Sealed design for inert gas filling Wire electrodes and miniature reference electrode OEM Potentiostats from IPS JPK offers third party potentiostats for electrochemistry applications together with NanoWizard systems. © IPS GmbH & Co. KG CellHesion® NanoWizard® ForceRobot® NanoTracker™ 17 9 Vibration and acoustic isolation Active vibration isolation from Accurion Key features Active Workstation 780 and 900 – active vibration isolation workstations i4 Series – compact table-top vibration isolation unit © Accurion GmbH Active vibration isolation from Table Stable Key features TS 150 Small size table-top vibration isolation system for loads up to 150kg TS 140 medium size table-top system for loads up to 140kg © Table Stable Ltd. Active air damping tables from TMC Key features For large setups such as AFM/Raman combinations or confocal microscopy For less-critical vibration level environments © Technical Manufacturing Corporation JPK acoustic enclosure Approved for high performance applications: acoustic hood from JPK for utmost stability and isolation. Key features Bio-acoustic enclosure for S1 level applications, S2 lab compatibility possible on demand Divisible in two parts for easy transport and setup Special wall design made by sandwich construction and acoustic foam for best damping results 2 front doors with windows Dimensions: 1100 mm × 1100 mm × 1250 mm Demands table with 1100 mm × 1100 mm area JPK base for acoustic enclosure Approved for high performance applications: base frame with top plate from JPK for utmost stability. Key features Dimensions: footprint 1100 mm × 1100 mm Height adjustable Welded steel base frame 100 mm MDF sandwich table top CellHesion® 18 NanoWizard® ForceRobot® NanoTracker™ 10 Optics accessories TopViewOptics™ The optical system for non transparent samples or substrates such as HOPG, mica, ceramics, metall etc. Key features For opaque samples Long working distance zoom lens from Navitar™ 2 mm - 400 µm field of view Köhler illumination, fiber light illumination Color CCD camera Easy positioning and focus adjustment TopViewOptics™ with boom stand The optical system for non transparent samples or substrates such as HOPG, mica, ceramics, implants etc. Can be used in combination with inverted microscopes. Key features For opaque samples Long working distance zoom lens from Navitar™ 2 mm - 400 µm field of view Köhler illumination, fiber light illumination Color CCD camera Easy positioning and focus adjustment TopViewOptics™ for use with inverted optical microscopes or the JPK BioMAT™ The optical system for non transparent samples or substrates such as HOPG, mica, ceramics, implants etc. Can be used in combination with inverted microscopes. Key features For opaque samples Long working distance zoom lens from Navitar™ 2 mm - 400 μm field of view Köhler illumination, fiber light illumination Color CCD camera Easy positioning and focus adjustment Raman reflector kit The optical system for Tip-Enhanced Raman Scattering (upright-TERS) on opaque samples. Key features For opaque samples Compatible with common inverted microscope Raman spectrometer setups CellHesion® NanoWizard® ForceRobot® Fits to the JPK Raman reflector kit sample holder and JPK TAO™ modules NanoTracker™ 19 Upright Fluorescence Microscope (UFM) Kit Enables the combined use of AFM and upright fluorescence zoom microscopy for co-localization experiments on opaque substrates. Fiber coupled detection module Allows the precise capture of light emitted by the SPM tip region into a fiber coupled detector. Suitable for home-build SNOM or other light emitting tip experiments. Key features Fiber coupler for 50 µm core multi-mode optical fiber (FC connector) Fits to c-mount equipped inverted optical microscopes Precise manual xy positioning of the fiber end Built-in CCD-camera system for the alignment of emission spot to fiber center Three way optical switch for viewing sample, fiber end, and semi-transparent overlay Inverted optical microscope configurations Research lines of inverted optical microscopes from Zeiss, Olympus, Nikon, and Leica. Ask the JPK applications team for more information. 11 OEM solutions and small parts Stand for AFM head Parking stand for all NanoWizard®, CellHesion® or ForceRobot® 300 heads. JPK sample holder for small samples Adaptor for standard sample holder. Holds magnetically fixed AFM metal stubs. CellHesion® 20 NanoWizard® ForceRobot® NanoTracker™ JPK cable anchorage pillow Heavy weight pillow for fixation of cables to reduce noise coupling. JPK cantilever changing tool Probe loading station for convenient cantilever exchange. JPK biocompatible glue For cantilever or sample fixation, easy removable and biocompatible. JPK spare parts Available on request. Springs for different cantilever holders Fluid cell seals Adapters Coverslips Tubing for fluid cells OEM syringe pumps JPK offers syringe pumps from World Precision Instruments (WPI) Aladdin AL-1000 Accepts syringe sizes: 1-60mL Programmable, economical, versatile Single pump Up to 100 pumps can be daisy-chained together via RS232 network © World Precision Instruments Ltd. Aladdin AL-4000 Accepts two different syringe sizes: smallest-60mL Programmable, economical, versatile Infuses and withdraws Pumping rates: 1.459mL/hr-127.2mL/min © World Precision Instruments Ltd. CellHesion® NanoWizard® ForceRobot® NanoTracker™ 21 CCD, EMCCD and CMOS cameras The DirectOverlay™ optical integration can be used with any camera. The following CCD cameras are preferred and can be offered directly by JPK. Andor® Technology iXon+ EMCCD Luca EMCCD Clara Interline CCD Zyla sCMOS © Andor Technology plc. Jenoptik ProgRes® cameras series, such as MFcool CFcool © Jenoptik AG The Imaging Source FireWire CCD color camera © Imaging Source Europe GmbH IDS Imaging Development Systems Compact USB CCD and CMOS cameras of type µEye® © IDS Imaging Development Systems GmbH CellHesion® 22 NanoWizard® ForceRobot® NanoTracker™ Photometrics Evolve™ EMCCD © Photometrics Hamamatsu Photonics K.K. ORCA CMOS © Hamamatsu Photonics K.K. OEM indentation solutions JPK offers different third party solutions for indentation. OEM materials testing modules for mechanical properties JPK offers different third party modules for in-situ AFM experiments under tensile, compression, bending or torsion load. OEM incubators for Live Cell experiments under controlled environments JPK offers different incubator models for LIVE CELL applications together with NanoWizard® or CellHesion® systems. OEM glove box systems for NanoWizard® AFMs The AFM system can be used in a glove box under controlled atmosphere. © GS Glovebox Systemtechnik GmbH © M. Braun Inertgas-Systeme GmbH Please see dedicated brochures or product notes for more detailed information on our website at www.jpk.com. CellHesion® NanoWizard® ForceRobot® NanoTracker™ 23 be more than a customer – be our partner Providing the best service and support worldwide is an essential part of JPK‘s philosophy. To accelerate this, JPK founded global offices in Singapore, Tokyo, Shanghai, Cambridge (UK), Paris (France) and Carpinteria (USA). JPK has the largest applications and service team specialized in life science and nano science applications to support your research. Our representatives in different countries are hand-selected to offer a service which meets the JPK quality standards. We achieve this through long-term partnership and by continuous training of applications scientists and service engineers. For more detailed questions our JPK applications team is just an email or phone call away from you. JPK WORLDWIDE JPK offers regular user trainings and courses for beginners‚ or experts in our imaging facilities worldwide. Here users can learn to operate new instruments or get tips and tricks ranging from experimental procedures to sample preparation. BERLIN HEADQUARTERS UK FRANCE USA SHANGHAI JAPAN SINGAPORE To find a representative in your region visit our website or call us. The handbook reflects a large variety of options and will grow further with latest developments. If you cannot find an accessory or operating mode in this handbook ask us directly. JPK is able to deliver customized solutions even for advanced applications. NanoWizard, CellHesion, TAO, BioMAT, NanoTracker, ForceRobot, Vortis, DirectOverlay, QI, HyperDrive, RampDesigner, ExperimentPlanner, ExperimentControl, ForceWatch, ForceWheel, FluidicsModule, HybridStage, BioCell, SmallCell, ECCell, HTHS, HCS, HCM, TopViewOptics, PetriDishHeater are trademarks or registered trademarks of JPK Instruments USA, Inc. All information and specifications are subject to change without notice. All rights reserved. © 2015, JPK Instruments USA, Inc. JPK Instruments USA, Inc. 4189 Carpinteria Avenue Suite 1 Carpinteria, CA 93013 tel.: +1 408 807 8878 usa.jpk.com This material shall not be used for an offer in: USA China Japan Europe & other regions Visit the JPK web site for more information and sign up for our eNewsletter. Web address: www.jpk.com Follow us on Facebook, Youtube and Linkedin.
Similar documents
NanoManipulation with NanoWizard® Technology
© JPK Instruments AG - all rights reserved – www.jpk.com This material shall not be used for an offer in: USA China Japan Europe & other regions
More information