BIOPOD series - FOGALE nanotech

BIOPOD series
The Protein Machine on the Bench
What makes the difference ?
Access Incredible Benefits with the BIOPOD
The protein machine on the bench
Introduction
The challenge for Process Development, Media optimization, Process optimization or clone screening lies in the limited
performance of available equipment using traditional scale–up or scale-down methods and equipments at bench scale. Culture
model as shake flask, well plates or disposable multi reactors do not provide so far the automated biological culture models or
recipes of culture to get faster results. The bioPOD is at bench scale, representative of a true scale up method and recipes able to
be transferred at a manufacturing stage with biological control on the process.
The Advantages
Compare to traditional scale-up or scale-down operation in fermentation, the biopod platform is offering significant
advantages:
- The fermentation is driven by a remote biological model
Reduce and Optimize R&D and Process Development Efforts
The bioPOD is a multi fermentation platform (POD) which enable multi-factorial fermentation runs based on
built-in biological recipes of culture for aerobic or anaerobic fermentation. The existing built-in recipes are
- Insight and Improvement of the robustness of a process ( for PAT and Quality by Design initiatives)
- Independent fermentor to study at same time different biological strategies and enhance the production of the
targeted protein
capable of performing fully automated batch, fed-batch or chemostat processes with pre-defined standard
- New design of recipes is validated before use in routine conditions by the end user
culture recipes but also able to be customized due to the modularity of the software platform.
- Culture are started any time without an operator when fermentor are used in an remote mode with a built in recipe
Compact Modular Design
Available in single, twin, triple and up to 64 units, the bioPOD design is based on the use of an ergonomic,
Unique Benefits
modular construction housing the local controller as well as additional gassing, pump and various modules.
This result in considerable savings in valuable benchtop space.
 Modular and Scalable from 100 ml up to 10 L Working Volume
The powerful local controller built in a tower arrangement or separate as modules (shelf stackable) offers a
Biological Recipes Monitoring the Fermentation Processes
comprehensive range of control and display facilities, like as up to 9 automated biological recipes and for
Optical and capacitance sensors Coupled for enhanced fermentation control
classic remote control cascadable PID control loops, x-stage cascade control for pO2, alarm monitoring, recipe
Fully Remote Process control customizable to Specific Fermentation Needs
control and password protection for critical system parameters
Ready to Use Induction models built in the Software Package
In addition there are software modules like specific biological
recipes customized and customizable to the targeted protein to
be expessed:
In combination with its software capabilities, the bioPOD is a
concept fully designed to change the way fermentor
performance must be achieved with less running time and less
Easy Connection to Sensors
complexity.
Simple Modular Design on the Bench or Wall Mounted
F200 unit with 2 x 100 ml bioreactors
Advanced Software
Proven Scalable Results
The software platform is designed to support biological models based on physical parameters
Scalability to larger conventional pilot fermentor has been proved with similar
to control automatically the growth of cells.
sensors, parameter control ability and performance.
Basically designed for protein production with E. coli the bioPOD can be also used with any
The bioPOD multi fermentor platform can be used to scale-up or scale down
bacteria strength and yeast such as Pichia Pastoris or Saccharomyces.
existing fermentation models (process optimization) with biological recipes built
This innovative and unique method of supervision lead to a concept where cells are managing
in the system, customised or other define biological model implemented into the
the process instead of using only the physical parameters as the way to manage and control a
software platform.
fermentation run.
Data storage of graphs and recipes with retrieval functions for an easy access to existing data.
Dual Peltier module with Built-In OD Sensors
Expandable with No Compromise
Do it all Faster and Better
BIOPOD
The protein machine on the bench
Specifications
Vessel
Biopod serie
Min Working Volume (liter)
Max Working Volume (liter)
Material
F200
F1
F3
0,03
0,2
0,4
0,1
1
2,9
Borosilicated Glass, 316 L S/S
Control unit
Control Panel
Calibration
Communication Port
Program Storage
Log Data Storage
Cabinet material
Dimension (inch)
Color Touch Panel
Local Easy Interface
RS 485
Full Embedded Software
Remote Controlling Software.
ABS
14.6'' x 28.8" x 9.1" (W x H x D)
Aeration
Inlet Gas Flowmeter
Sparger
TMFC for Air/O2 up to 2.5 VVM
Sintered
Temperature
Control System
Range
Resolution
Probe
Control mode
Chiller, Heating Belt
4 or 14 C up to 65 C
0,1 C
Platinum RTD Probe (PT100)
Manual, Auto (PID), Remote
Agitation
Drive
Speed Range
Resolution
Impeller
Control mode
F200 system
Removable Top Brushless Motor
30 – 1200 RPM
1 RPM
1,2 or 3 Rushton Impellers
Manual, Auto
Gas lift
pH
Range
Resolution
Electrode
Control Mode
2 – 12 pH
0,01 Unit
Gel Filled, Autoclavable
On/Off, Adjustable PID 1Way, 2 Ways, Manual, Auto, Remote
DO
Range
Resolution
Probe
Control Mode
0 – 470 % (100% for air)
0,1 %
Polarographic or optical, Autoclavable
Adjustable PID, Cascade, Manual, Auto, Remote
OD
Range
Resolution
Probe
Control Mode
F200 system
0 – 4 AU
Range
Resolution
Probe
Control Mode
1g/L – 200g/L DCW for Yeast, Bacteria and Fungi
5g/L min with Highly Aerated Media
0 - 700pF/m Max Permittivity; 0.5 – 100mS/cm Conductance
0.02g/L DCW for Yeast
Capacitive, Autoclavable, USP Class VI Materials.
Adjustable via Recipe Control
Foam
Probe
Control Mode
316 L S/S Height Adjustable
ON/OFF Control
Peristaltic pumps
Pump number
6 Built-in Pumps, 2 External pumps
Motor Type
Pulse Driven Motor for
Flow Rate
minimum 0.15μL each 46h up to nominal flow rate.
Speed
47 RPM, 100RPM (other on request)
Nominal Flow rate
3.2ml/min (built in), 75ml/min (external). (other on request)
Resolution
0.15μL
Control mode
On/Off, Manual, Auto, Remote
Pump(s) can be Assigned for Acid, Base, Inoculum, Inducer, Anti-Foam and Substrates
Off Gas
Device type
Glass or 316 L S/S Condenser
Utility requirement
Power Supply
Water
Gas
110V or 230V; 50/60 Hz with Safety Cut off Switch
No Water supply Connection with chiller
1.2 bar Max. Medical Grade, dry, oil free and filtered
USA / Asia
FOGALE biotech, Inc.
Cambridge, MA 02140
Phone: (818) 206 8525
[email protected]
F10
3,5
11
Optical, Autoclavable, USP Class VI Materials.
Adjustable via Recipe Control
Integrated external sensor
Europe
FOGALE nanotech
Nîmes, France
Phone: +33 (0)4 66 62 05 55
[email protected]
© Edition 2011/05-TUS - FOGALE biotech. All right reserved
Capacitance
F5
1
6