The Phenion® Full-Thickness Skin Model
Transcription
The Phenion® Full-Thickness Skin Model
The Phenion® Full-Thickness Skin Model The Phenion® Full-Thickness (FT) Skin Model resembles native human skin in its histological architecture as well as in displaying a wide spectrum of corresponding physiological parameters. Bioartificial human skin for R&D and safety assessment commercially available since 2006 Constructed on the basis of a proprietary bovine collagen scaffold, the Phenion® FT Skin Model features morphological equivalence with human skin and allows convenient handling in research applications. The skin model production lab is located at the Biological and Clinical Research (BCR) Department within Henkel Beauty Care Technologies, Düsseldorf. To cover the needs of genotoxicity assessment of chemicals we are currently working on the validation of the Phenion® FT Skin Model for the Comet Assay. www.henkel.com Phenion® FT Skin Models The reliable tool for toxicological and efficacy studies Features of the skin equivalent: Consists of human primary fibroblasts and keratinocytes from single donor origin Cell batches are carefully selected for optimal 3D-tissue composition Cornified surface Multi-layered epidermis Both skin compartments exhibit physiologically relevant parameters: Keratinocyte differentiation into a multi-layered stratified epithelium is associated with the expression of specific markers like cytokeratin 10, filaggrin, transglutaminase and involucrin. Epidermal and dermal differentiation At the dermal-epidermal junction zone crucial basement membrane proteins, like collagen IV and VII and laminin-5 support epithelial adhesion. Generation of the elastic system During maturation pivotal connective tissue components including collagenous and elastic networks consisting of proteins like elastin and fibrillin-1 - are established in the dermis. Penetration The matured dermis displays excellent elastic fiber network Circular tissue with a diameter of 1.3 cm Long-term cultivation for at least 10 days allows repeated treatments Suitable for topical and systemical substance application The Phenion® FT Skin Model allows investigation of the following exemplary endpoints: Safety and Efficacy testing of substances / products Cytotoxicity Genotoxicity Skin Metabolism Wound healing Your contact for requests and ordering: Lars Vierkotten Skin Model Production E-mail [email protected] Phone (+49) 221 797-9744 The Phenion® Full-Thickness Skin Model Bioartificial human skin for R&D and safety assessment – commercially available since 2006 References Reisinger K, Bartel M, Blatz V, Brinkmann J, Downs TR, Engels U, Fischer A, Henkler F, Hoffmann S, Jeschke S, Kühnl J, Krul C, Liebsch M, Luch A, Pirow R, Reus A, Schulz M, Pfuhler S 3D Skin Comet assay: Status quo of the ongoing validation 9th World Congress on Alternatives and Animal Use in Life Sciences 2014 Wiegand C, Hewitt N, Merk HF, Reisinger K. Dermal xenobiotic metabolism: A comparison between human native skin, four in vitro skin test systems and a liver system Skin Pharmacol Physiol 2014. 27:263–275. Pfuhler S, Bartel M, Blatz V, Brinkmann J, Downs TR, Engels U, Fischer A, Henkler F, Jeschke S, Krul C, Liebsch M, Luch A, Pirow R, Reus A, Schulz M, Reisinger K. 3D Skin Comet assay validation using full thickness tissues: Update on the ongoing validation ICEM 2013 Bätz FM, Klipper W, Korting HC, Henkler F, Landsiedel R, Luch A, von Fritschen U, Weindl G, Schäfer-Korting M. Esterase activity in excised and reconstructed human skin - biotransformation of rednicarbate and the model dye fluorescein diacetate. Eur J Pharm Biopharm. 2013. 84:374-85. Jäckh C, Blatz V, Fabian E, Guth K, van Ravenzwaay B, Reisinger K, Landsiedel R. Characterization of enzyme activities of Cytochrome P450 enzymes, Flavin-dependent monooxygenases, N-acetyltransferases and UDP-glucuronyltransferases in human reconstructed epidermis and full-thickness skin models. Toxicol In Vitro. 2011. 25:1209-14. Ackermann K, Borgia SL, Korting HC, Mewes KR, Schäfer-Korting M. The Phenion full-thickness skin model for percutaneous absorption testing. Skin Pharmacol Physiol. 2010. 23:105-12. Meloni M, Farina A, de Servi B. Molecular modifications of dermal and epidermal biomarkers following UVA exposures on reconstructed full-thickness human skin. Photochem Photobiol Sci. 2010. 9:439-47. Schoepe S, Schäcke H, Bernd A, Zöller N, Asadullah K. Identification of novel in vitro test systems for the determination of glucocorticoid receptor ligand-induced skin atrophy. Skin Pharmacol Physiol. 2010. 23:139-51. Zöller NN, Kippenberger S, Thaçi D, Mewes K, Spiegel M, Sättler A, Schultz M, Bereiter-Hahn J, Kaufmann R, Bernd A. Evaluation of beneficial and adverse effects of glucocorticoids on a newly developed fullthickness skin model. Toxicol In Vitro. 2008. 22:747-59. Mewes KR, Raus M, Bernd A, Zöller NN, Sättler A, Graf R. Elastin expression in a newly developed full-thickness skin equivalent. Skin Pharmacol Physiol. 2007. 20:85-95. Described in Groeber F, Holeiter M, Hampel M, Hinderer S, Schenke-Layland K. Skin tissue Engineering - in vivo and in vitro applications. Adv Drug Deliv Rev. 2011. 63:352-66. De Wever B, Petersohn D, Mewes KR Overview of human three-dimensional (3D) skin models used for dermal toxicity assessment, Part 1. Household and Personal Care Today. 2013. 8:18-22 Contact To request information regarding the Phenion® FT Skin Model or to place orders you may contact Lars Vierkotten Skin Model Production e-mail [email protected] phone +49 (0)211/797-9744
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