The Phenion® Full-Thickness Skin Model

The Phenion®
Full-Thickness
Skin Model
The Phenion® Full-Thickness
(FT) Skin Model resembles
native human skin in its
histological architecture as
well as in displaying a wide
spectrum of corresponding
physiological parameters.
Bioartificial human
skin for R&D and
safety assessment commercially
available since 2006
Constructed on the basis of a proprietary
bovine collagen scaffold, the Phenion® FT
Skin Model features morphological
equivalence with human skin and allows
convenient
handling
in
research
applications.
The skin model production lab is located
at the Biological and Clinical Research
(BCR) Department within Henkel Beauty
Care Technologies, Düsseldorf.
To cover the needs of genotoxicity
assessment of chemicals we are currently
working on the validation of the Phenion®
FT Skin Model for the Comet Assay.
www.henkel.com
Phenion® FT
Skin Models
The reliable tool
for toxicological and
efficacy studies
Features of the skin equivalent:
Consists of human primary fibroblasts and
keratinocytes from single donor origin
Cell batches are carefully selected for
optimal 3D-tissue composition
Cornified surface
Multi-layered epidermis
Both skin compartments
exhibit physiologically
relevant parameters:
Keratinocyte differentiation
into
a
multi-layered
stratified
epithelium
is
associated
with
the
expression
of
specific
markers like cytokeratin 10,
filaggrin, transglutaminase
and involucrin.
Epidermal and dermal differentiation
At the dermal-epidermal
junction zone crucial basement membrane proteins,
like collagen IV and VII and
laminin-5 support epithelial
adhesion.
Generation of the elastic system
During maturation pivotal
connective tissue components including collagenous
and elastic networks consisting of proteins like
elastin and fibrillin-1 - are
established in the dermis.
Penetration
The matured dermis displays excellent
elastic fiber network
Circular tissue with a diameter of 1.3 cm
Long-term cultivation for at least 10 days
allows repeated treatments
Suitable for topical and systemical
substance application
The Phenion® FT Skin Model allows
investigation of the following exemplary
endpoints:
Safety and Efficacy testing of substances /
products
Cytotoxicity
Genotoxicity
Skin Metabolism
Wound healing
Your contact for requests and ordering:
Lars Vierkotten
Skin Model Production
E-mail [email protected]
Phone (+49) 221 797-9744
The Phenion® Full-Thickness
Skin Model
Bioartificial human skin for R&D and safety
assessment – commercially available since 2006
References
Reisinger K, Bartel M, Blatz V, Brinkmann J, Downs TR, Engels U, Fischer A, Henkler F, Hoffmann S,
Jeschke S, Kühnl J, Krul C, Liebsch M, Luch A, Pirow R, Reus A, Schulz M, Pfuhler S
3D Skin Comet assay: Status quo of the ongoing validation
9th World Congress on Alternatives and Animal Use in Life Sciences 2014
Wiegand C, Hewitt N, Merk HF, Reisinger K.
Dermal xenobiotic metabolism: A comparison between human native skin, four in vitro skin
test systems and a liver system
Skin Pharmacol Physiol 2014. 27:263–275.
Pfuhler S, Bartel M, Blatz V, Brinkmann J, Downs TR, Engels U, Fischer A, Henkler F, Jeschke S, Krul
C, Liebsch M, Luch A, Pirow R, Reus A, Schulz M, Reisinger K.
3D Skin Comet assay validation using full thickness tissues: Update on the ongoing validation
ICEM 2013
Bätz FM, Klipper W, Korting HC, Henkler F, Landsiedel R, Luch A, von Fritschen U, Weindl G,
Schäfer-Korting M.
Esterase activity in excised and reconstructed human skin - biotransformation of rednicarbate
and the model dye fluorescein diacetate.
Eur J Pharm Biopharm. 2013. 84:374-85.
Jäckh C, Blatz V, Fabian E, Guth K, van Ravenzwaay B, Reisinger K, Landsiedel R.
Characterization of enzyme activities of Cytochrome P450 enzymes,
Flavin-dependent monooxygenases, N-acetyltransferases and
UDP-glucuronyltransferases in human reconstructed epidermis and full-thickness
skin models.
Toxicol In Vitro. 2011. 25:1209-14.
Ackermann K, Borgia SL, Korting HC, Mewes KR, Schäfer-Korting M.
The Phenion full-thickness skin model for percutaneous absorption testing.
Skin Pharmacol Physiol. 2010. 23:105-12.
Meloni M, Farina A, de Servi B.
Molecular modifications of dermal and epidermal biomarkers following UVA exposures on
reconstructed full-thickness human skin.
Photochem Photobiol Sci. 2010. 9:439-47.
Schoepe S, Schäcke H, Bernd A, Zöller N, Asadullah K.
Identification of novel in vitro test systems for the determination of glucocorticoid receptor
ligand-induced skin atrophy.
Skin Pharmacol Physiol. 2010. 23:139-51.
Zöller NN, Kippenberger S, Thaçi D, Mewes K, Spiegel M, Sättler A, Schultz M,
Bereiter-Hahn J, Kaufmann R, Bernd A.
Evaluation of beneficial and adverse effects of glucocorticoids on a newly developed fullthickness skin model.
Toxicol In Vitro. 2008. 22:747-59.
Mewes KR, Raus M, Bernd A, Zöller NN, Sättler A, Graf R.
Elastin expression in a newly developed full-thickness skin equivalent.
Skin Pharmacol Physiol. 2007. 20:85-95.
Described in
Groeber F, Holeiter M, Hampel M, Hinderer S, Schenke-Layland K.
Skin tissue Engineering - in vivo and in vitro applications.
Adv Drug Deliv Rev. 2011. 63:352-66.
De Wever B, Petersohn D, Mewes KR
Overview of human three-dimensional (3D) skin models used for dermal toxicity assessment,
Part 1.
Household and Personal Care Today. 2013. 8:18-22
Contact
To request information regarding the Phenion® FT Skin Model or to place orders you may contact
Lars Vierkotten
Skin Model Production
e-mail [email protected]
phone +49 (0)211/797-9744